MD17G1201100.v1.1

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009628
Physical Location & Seq
Reverse (-)
24297862 .. 24301083
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1201100.v1.1.491

Sequence Viewer

Length: 3222 bp
ATGATGAAGTTTGCATGTTTGGTTGTTGTGGTTTGGAGCGTTTATGGGTTGCTGTTTAGTTTGGGGCTCACTTATTCAGAACAATCAGAACCAGGATTCGAATTTACAAATGGAAGAAGTGGAAGCCCTGTTACTTACAGCTATGATCGGATCGATGAAGTGAAGAAAGAATGTCGTTTTGTTCTTTCTTCTGCTTCTGAACTGAAAGCTGAAGATAATCGAATTTATAGCATAAAAACTGAGCTCTTTTTTGTCAATGGGGACTGGAGGCAGGAGGTGGGTGATGCTCCAATTATCCCATTTGATGATAGAGAATTTGAAATGAGCTTGGTGGAGGATATTAATCGTACGTCATCGAACTTGGTTTCATTTTGGGTTATGGATGTTGATCGTGCCCATCGATCCAAGAAGTCAGTGAGTGTTAGTGGGGTCATGGTTTTGGGCATTACTAAAGGCGGTAGCTTTGCGGATTATAGGTACGAAGGAAATCCAAAATTTCAAATATGGCCCGGCCATTCTCAGCTCACAGTTTCCTTCCAAGGGATTTACACTGAATCCAAGAAAAATGGTGGGGAAAGAGTAATGTGTTTGTTGGGGAGTACAATGTTGCCGTCCCGGGAGACTGATTCTGCTAATCCCTGGGAGTGGTTGAAGGCTTCTGATGAAAATTACGACCAACCTCCTCTTTCCGAAGATGATCAGATTCTACTAATTCTTCATTATCCAGCGACTTTCTCATTGACAAATAGGGTTATCCAAGGAGAATTAAGAAGCTTAAATTCAAAGTCAAACTCAAAGTACTTTGATACGGTTCACATTTCATCACAGTTGGGAAAGTCCGCAACGTATGAGTTTGGAGCTGAAAAGATCGTGTCAAGAGCATGTGATCCGTACCCTTCTAATGATAACTTGATATATGGTGGCATTAGCATTTACAAAGGTCCTTCGTTTTGTGAAATCCTCCAAGAAGTTACACGGGAACAAGCTTTCACTGTCTTGCCAAACTGGAGATGTAATTTCCCGGGTGATTTTTGCAGCAAGCTGGGTCCATTTGTGGCAGATAAAGAGATTAGGGCATCAAATGGAAGCTTTAAAGGTGTTAAAATTTTTATGCAGGATATCAAGTGTGAACAAAAAAATGCCGCAGGAAATGCCAGTTCAGCAACGGTTTCAGCTGTGTTTAGAGCTGTTTCCCCGCTAGAGAATGAGTATACTGCGGCAAAGAGATCTGGGCTTAACAACATGACTGTTGCAGCGGAAGGAATATGGAAGTCTACCAGTGGGCAGCTTTGCATGGCTGGTTGCCTTGGACTTGCTGATGTTCAAGGAGGTCAATGCAATAGTCGAATCTGCCTGTACATACCAGTCTCTTTTTCCATAAAGCAACGAAGCATTATTTATGGAAGTTTATCCAGCATCAATAACAGTGGTGCATTGTATTTTCCTCTCTCCTTTGAAAAGCTAGTGCAACCTACAGAGCTGTGGAATTACTTCAGAACCTCCAGTCCAAACTACAGGTACACAAAACTTGATTCAGCTGCTATTATCTTGGAAAAAAATGAGGCCTTTAGTGTTGGAACTGTCATAAAAAAATCACTGCTGAATTTTCCCAAACTGGAAGATACAGAATCTTTTCAAGTCAGTCTTTCTCTTCTCTCGGAAGACCTAACCCTTCACGAGTCTGCATTTCTCGATCCAATCCGAGATCTTCACTCTCCAAGAATAGATATTCAGATGGAGATTCTGTCAGTCGGTCCCTTGTTTGGGCGCTTTTGGTCTCCACAAAACAGTTCAACTGCAGAAGAGGGGACTCCTTACCACACGAAGGCTGAATACACTGAAAAGCAGCTCCTTATGAATATATCTGCACAACTCACCATCACTGGGAAAGGTTTCAGCAATTTTTCAGTTCTCTTTCTGGAGGGTCTTTATGACCCACATGTGGGAAAGATGTATCTAGTTGGCTGCAGGGATGTTCGAGCCTCGTGGAAGATCTTGTATGAGAGCATGGATCTTGAGGCTGGGCTTGATTGTTTAATTGAGGTGGTTGTGTCGTATCCTCCCACTACATCTCTATGGTTAGGCAATCCAACAGCAAGTATTTCCGTAGCCAGTCAAAGGAATGAAGATGACCCCCTTTTCTTTAGTACAGTCAAGCTCAGAACTCTTCCAATTATGTACAGAAAACAGCGGGAAAGCATCCTCTCTCGTCGAGGCATTGAGGGAATCCTCCGGATTTTGACTCTTTCCCTGGCAATTTCTGGCATTTTAAGCCAACTGTTTTACATCAGGCATAATGTGGATACTGTTCCTTACATGTCTCTGGTCATGCTAGGTATCCAAGCCATTGGGTACAGCATTCCTCTGGTCACTGATGCTGAAGCGCTCTTCAAGAGGATATCTTCCGACTCCAATGCAACATCTTCGTATGACCTTGAGAACAATCAGTGGTTCCATATACTTGATTACACGGTAAAGTTTCTGGTGATGGTTTCACTTTTACTCACTTTAAGGCTTTGTCAGAAGGTGTGGAAATCTCGAATCAGATTACTTACGCAAACTCCTCTTGAGCCACATCGTGTACCTAGTGATAAACGGGTGCTTCTGACTACCTTTGCAATACATTTTATTGGCTATATCATTGTTTTAATCATTCATAGTATGACAACTAGCAGAAGATATATTCGGACAAAAAGCTATAGAATAGCTAGAGCAAATTCTCATGCACTGTGGGAGTGGGAAACTGAGCTAGAGGAGTATGTCGGTCTTGTTCAAGATTTTTTCCTTCTCCCACAAATCATTGGCAACCTTGTGTGGCAGATGGATTGCAAACCGCTCAGAAAGTTCTATTTCTTTGCAATCACATTGGTCAGAATCTTCCCTCACATTTATGATTATATTAGAGCTCCTGCTCTCAATCCTTACTTTGCAGAGGACTACGAGCTTGTCAACCCGACTATGGATTTTTACTCCAAGTTTGGGGATATTGCCATACCGGTTACAGCAATCATACTTGCAGGTATAGTCTATGCTCAGCAGAGGTGGAGTTACGAGAGGATCAGCCAGACTCTTACCGTGGGGCAATATAGGCTTCTTCCTTTGGGTTCGAGAATGTATGAGAGGTTGCCTTCAAGTTCCATGGCATTTGAAGCTGAGCTTGTTTCGAGTGTCAATGGTAATGCTAGACACGAGAAAGAGAAAGAGAATGATGATGGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1074

Amino Acids

121.33

Weight (kDa)

6.01

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 54 - 272 5.8e-38 DUF2921 lipocalin-like domain
DUF2921_N PF25333 291 - 482 5.9e-43 DUF2921 lipocalin-like domain
DUF2921_N PF25333 523 - 718 2.6e-32 DUF2921 lipocalin-like domain
DUF2921 PF11145 730 - 1017 5.4e-59 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3008
AccBSI CCGCTC 1 cut(s) 2836
AccI GTMKAC 2 cut(s) 1211, 1274
AccIII TCCGGA 1 cut(s) 2232
AciI CCGC 9 cut(s) 456, 467, 840, 1143, 1196, 1217, 1256, 2191, 2834
AclWI GGATC 6 cut(s) 158, 396, 881, 1688, 2019, 3065
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 8 cut(s) 101, 222, 314, 494, 778, 1104, 1603, 2716
AcuI CTGAAG 3 cut(s) 231, 1477, 2400
AdeI CACNNNGTG 1 cut(s) 2579
AfeI AGCGCT 1 cut(s) 2385
AflIII ACRYGT 2 cut(s) 1939, 2316
AgeI ACCGGT 1 cut(s) 2995
AjnI CCWGG 3 cut(s) 91, 638, 2250
AloI GAACNNNNNNTCC 2 cut(s) 1489, 1521
Alw21I GWGCWC 2 cut(s) 246, 2908
Alw26I GTCTC 4 cut(s) 614, 1372, 1782, 2325
AlwI GGATC 6 cut(s) 158, 396, 881, 1688, 2019, 3065
Ama87I CYCGRG 2 cut(s) 615, 1021
Aor13HI TCCGGA 1 cut(s) 2232
Aor51HI AGCGCT 1 cut(s) 2385
AoxI GGCC 3 cut(s) 506, 511, 1563
ApeKI GCWGC 6 cut(s) 1035, 1253, 1285, 1538, 1846, 1965
ApoI RAATTY 8 cut(s) 101, 222, 314, 494, 778, 1104, 1603, 2716
AseI ATTAAT 1 cut(s) 342
AsiGI ACCGGT 1 cut(s) 2995
Asp700I GAANNNNTTC 3 cut(s) 1490, 1632, 1892
AspLEI GCGC 2 cut(s) 1770, 2386
AspS9I GGNCC 4 cut(s) 507, 941, 1046, 1754
AsuC2I CCSGG 5 cut(s) 510, 616, 617, 1022, 1023
AsuHPI GGTGA 4 cut(s) 293, 1037, 1867, 2497
AsuII TTCGAA 1 cut(s) 99
AvaI CYCGRG 2 cut(s) 615, 1021
AvaII GGWCC 3 cut(s) 941, 1046, 1754
BaeGI GKGCMC 1 cut(s) 397
BanII GRGCYC 3 cut(s) 69, 246, 2908
BarI GAAGNNNNNNTAC 4 cut(s) 115, 147, 1817, 1849
BauI CACGAG 3 cut(s) 1676, 1984, 3188
BbsI GAAGAC 1 cut(s) 1668
Bbv12I GWGCWC 2 cut(s) 246, 2908
BbvI GCAGC 6 cut(s) 1047, 1265, 1297, 1525, 1858, 1952
BccI CCATC 6 cut(s) 405, 1729, 1886, 2482, 2815, 3206
BceAI ACGGC 1 cut(s) 595
BcgI CGANNNNNNTGC 4 cut(s) 2396, 2406, 2430, 2440
BciT130I CCWGG 3 cut(s) 93, 640, 2252
BciVI GTATCC 3 cut(s) 2067, 2296, 2348
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 5 cut(s) 510, 616, 617, 1022, 1023
BcoDI GTCTC 4 cut(s) 614, 1372, 1782, 2325
BfaI CTAG 9 cut(s) 1199, 1463, 1958, 2333, 2586, 2670, 2709, 2750, 3183
BfmI CTRYAG 5 cut(s) 1473, 1513, 1797, 1966, 2698
BfoI RGCGCY 2 cut(s) 1771, 2387
BfuAI ACCTGC 1 cut(s) 3008
BfuI GTATCC 3 cut(s) 2067, 2296, 2348
BglII AGATCT 3 cut(s) 1226, 1705, 1992
BisI GCNGC 8 cut(s) 1036, 1143, 1218, 1254, 1286, 1539, 1847, 1966
BlpI GCTNAGC 2 cut(s) 3033, 3153
BlsI GCNGC 8 cut(s) 1037, 1144, 1219, 1255, 1287, 1540, 1848, 1967
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 8 cut(s) 93, 510, 616, 617, 640, 1022, 1023, 2252
Bme18I GGWCC 3 cut(s) 941, 1046, 1754
BmeT110I CYCGRG 2 cut(s) 615, 1021
BmgT120I GGNCC 4 cut(s) 507, 941, 1046, 1754
BmiI GGNNCC 3 cut(s) 1047, 1756, 2453
BmrFI CCNGG 8 cut(s) 93, 510, 616, 617, 640, 1022, 1023, 2252
BmrI ACTGGG 1 cut(s) 1893
BmsI GCATC 5 cut(s) 274, 1085, 1425, 2208, 2365
BmuI ACTGGG 1 cut(s) 1893
BpiI GAAGAC 1 cut(s) 1668
BpmI CTGGAG 4 cut(s) 286, 1027, 1486, 1940
Bpu1102I GCTNAGC 2 cut(s) 3033, 3153
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 2036, 2456, 2588
BpuMI CCSGG 5 cut(s) 510, 616, 617, 1022, 1023
Bsa29I ATCGAT 2 cut(s) 153, 400
BsaBI GATNNNNATC 2 cut(s) 342, 387
BsaI GGTCTC 1 cut(s) 1782
BsaWI WCCGGW 2 cut(s) 2232, 2995
BsaXI ACNNNNNCTCC 4 cut(s) 1730, 1760, 2545, 2575
Bse118I RCCGGY 1 cut(s) 2995
Bse1I ACTGG 9 cut(s) 269, 1010, 1155, 1278, 1364, 1503, 1620, 1888, 2112
Bse8I GATNNNNATC 2 cut(s) 342, 387
BseAI TCCGGA 1 cut(s) 2232
BseBI CCWGG 3 cut(s) 93, 640, 2252
BseCI ATCGAT 2 cut(s) 153, 400
BseGI GGATG 3 cut(s) 388, 1978, 2199
BseJI GATNNNNATC 2 cut(s) 342, 387
BseMII CTCAG 7 cut(s) 231, 533, 2173, 2736, 2851, 3047, 3144
BseNI ACTGG 9 cut(s) 269, 1010, 1155, 1278, 1364, 1503, 1620, 1888, 2112
BseRI GAGGAG 3 cut(s) 672, 2553, 2768
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 6 cut(s) 1047, 1265, 1297, 1525, 1858, 1952
BseYI CCCAGC 2 cut(s) 1042, 2021
BsgI GTGCAG 1 cut(s) 1851
BshFI GGCC 3 cut(s) 508, 513, 1565
BshTI ACCGGT 1 cut(s) 2995
BshVI ATCGAT 2 cut(s) 153, 400
BsiHKAI GWGCWC 2 cut(s) 246, 2908
BsiHKCI CYCGRG 2 cut(s) 615, 1021
BsiSI CCGG 5 cut(s) 510, 616, 1022, 2233, 2996
BsiWI CGTACG 1 cut(s) 347
BslFI GGGAC 4 cut(s) 275, 598, 1740, 1822
BsmAI GTCTC 4 cut(s) 614, 1372, 1782, 2325
BsmFI GGGAC 4 cut(s) 275, 598, 1740, 1822
BsmI GAATGC 1 cut(s) 2358
BsnI GGCC 3 cut(s) 508, 513, 1565
Bso31I GGTCTC 1 cut(s) 1782
BsoBI CYCGRG 2 cut(s) 615, 1021
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 4 cut(s) 69, 246, 397, 2908
Bsp13I TCCGGA 1 cut(s) 2232
Bsp1407I TGTACA 2 cut(s) 1356, 2178
Bsp1720I GCTNAGC 2 cut(s) 3033, 3153
Bsp19I CCATGG 1 cut(s) 3138
BspACI CCGC 9 cut(s) 456, 467, 840, 1143, 1196, 1217, 1256, 2191, 2834
BspANI GGCC 3 cut(s) 508, 513, 1565
BspCNI CTCAG 7 cut(s) 232, 532, 2172, 2737, 2850, 3046, 3145
BspDI ATCGAT 2 cut(s) 153, 400
BspEI TCCGGA 1 cut(s) 2232
BspLI GGNNCC 3 cut(s) 1047, 1756, 2453
BspMAI CTGCAG 2 cut(s) 1801, 1970
BspMI ACCTGC 1 cut(s) 3008
BspPI GGATC 6 cut(s) 158, 396, 881, 1688, 2019, 3065
BspQI GCTCTTC 1 cut(s) 2393
BspT104I TTCGAA 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 1782
BsrBI CCGCTC 1 cut(s) 2836
BsrFI RCCGGY 1 cut(s) 2995
BsrGI TGTACA 2 cut(s) 1356, 2178
BsrI ACTGG 9 cut(s) 269, 1010, 1155, 1278, 1364, 1503, 1620, 1888, 2112
BssAI RCCGGY 1 cut(s) 2995
BssNAI GTATAC 1 cut(s) 1212
BssSI CACGAG 3 cut(s) 1676, 1984, 3188
BssT1I CCWWGG 4 cut(s) 538, 757, 1306, 3138
Bst1107I GTATAC 1 cut(s) 1212
Bst2BI CACGAG 3 cut(s) 1676, 1984, 3188
Bst2UI CCWGG 3 cut(s) 93, 640, 2252
Bst6I CTCTTC 4 cut(s) 1656, 1797, 2172, 2393
BstAPI GCANNNNNTGC 1 cut(s) 1151
BstAUI TGTACA 2 cut(s) 1356, 2178
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 1040
BstDEI CTNAG 7 cut(s) 240, 519, 2159, 2745, 2837, 3033, 3153
BstDSI CCRYGG 2 cut(s) 3075, 3138
BstF5I GGATG 3 cut(s) 388, 1978, 2199
BstH2I RGCGCY 2 cut(s) 1771, 2387
BstHHI GCGC 2 cut(s) 1770, 2386
BstMAI GTCTC 4 cut(s) 614, 1372, 1782, 2325
BstMWI GCNNNNNNNGC 5 cut(s) 1151, 1160, 2271, 3088, 3149
BstNI CCWGG 3 cut(s) 93, 640, 2252
BstNSI RCATGY 4 cut(s) 18, 885, 1943, 2320
BstSCI CCNGG 8 cut(s) 91, 508, 614, 615, 638, 1020, 1021, 2250
BstSFI CTRYAG 5 cut(s) 1473, 1513, 1797, 1966, 2698
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 6 cut(s) 1047, 1265, 1297, 1525, 1858, 1952
BstV2I GAAGAC 1 cut(s) 1668
BstX2I RGATCY 4 cut(s) 1226, 1705, 1992, 2011
BstXI CCANNNNNNTGG 1 cut(s) 2348
BstYI RGATCY 4 cut(s) 1226, 1705, 1992, 2011
BstZ17I GTATAC 1 cut(s) 1212
Bsu15I ATCGAT 2 cut(s) 153, 400
BsuI GTATCC 3 cut(s) 2067, 2296, 2348
BsuRI GGCC 3 cut(s) 508, 513, 1565
BsuTUI ATCGAT 2 cut(s) 153, 400
BtgI CCRYGG 2 cut(s) 3075, 3138
BtsCI GGATG 3 cut(s) 388, 1978, 2199
BtsI GCAGTG 1 cut(s) 1595
BveI ACCTGC 1 cut(s) 3008
Cac8I GCNNGC 1 cut(s) 1040
CfoI GCGC 2 cut(s) 1770, 2386
Cfr10I RCCGGY 1 cut(s) 2995
Cfr13I GGNCC 4 cut(s) 507, 941, 1046, 1754
Cfr9I CCCGGG 2 cut(s) 615, 1021
ClaI ATCGAT 2 cut(s) 153, 400
CspAI ACCGGT 1 cut(s) 2995
CspCI CAANNNNNGTGG 2 cut(s) 1408, 1443
DdeI CTNAG 7 cut(s) 240, 519, 2159, 2745, 2837, 3033, 3153
DraI TTTAAA 1 cut(s) 1093
DraIII CACNNNGTG 1 cut(s) 2579
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 4 cut(s) 1656, 1797, 2172, 2393
EarI CTCTTC 4 cut(s) 1656, 1797, 2172, 2393
Ecl136II GAGCTC 2 cut(s) 244, 2906
Eco130I CCWWGG 4 cut(s) 538, 757, 1306, 3138
Eco147I AGGCCT 1 cut(s) 1565
Eco24I GRGCYC 3 cut(s) 69, 246, 2908
Eco31I GGTCTC 1 cut(s) 1782
Eco32I GATATC 2 cut(s) 1120, 2400
Eco47I GGWCC 3 cut(s) 941, 1046, 1754
Eco47III AGCGCT 1 cut(s) 2385
Eco53kI GAGCTC 2 cut(s) 244, 2906
Eco57I CTGAAG 3 cut(s) 231, 1477, 2400
Eco88I CYCGRG 2 cut(s) 615, 1021
EcoICRI GAGCTC 2 cut(s) 244, 2906
EcoO109I RGGNCCY 1 cut(s) 941
EcoRII CCWGG 3 cut(s) 91, 638, 2250
EcoRV GATATC 2 cut(s) 1120, 2400
EcoT14I CCWWGG 4 cut(s) 538, 757, 1306, 3138
EcoT38I GRGCYC 3 cut(s) 69, 246, 2908
ErhI CCWWGG 4 cut(s) 538, 757, 1306, 3138
FalI AAGNNNNNCTT 4 cut(s) 1629, 1661, 3141, 3173
FaqI GGGAC 4 cut(s) 275, 598, 1740, 1822
FauI CCCGC 2 cut(s) 1203, 2184
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 2 cut(s) 1211, 1274
Fnu4HI GCNGC 8 cut(s) 1036, 1143, 1218, 1254, 1286, 1539, 1847, 1966
FokI GGATG 3 cut(s) 395, 1985, 2186
FriOI GRGCYC 3 cut(s) 69, 246, 2908
Fsp4HI GCNGC 8 cut(s) 1036, 1143, 1218, 1254, 1286, 1539, 1847, 1966
FspBI CTAG 9 cut(s) 1199, 1463, 1958, 2333, 2586, 2670, 2709, 2750, 3183
GlaI GCGC 2 cut(s) 1769, 2385
GluI GCNGC 8 cut(s) 1036, 1143, 1218, 1254, 1286, 1539, 1847, 1966
GsaI CCCAGC 2 cut(s) 1046, 2025
GsuI CTGGAG 4 cut(s) 286, 1027, 1486, 1940
HaeII RGCGCY 2 cut(s) 1771, 2387
HaeIII GGCC 3 cut(s) 508, 513, 1565
HapII CCGG 5 cut(s) 510, 616, 1022, 2233, 2996
HhaI GCGC 2 cut(s) 1770, 2386
Hin6I GCGC 2 cut(s) 1768, 2384
HinP1I GCGC 2 cut(s) 1768, 2384
HincII GTYRAC 1 cut(s) 2950
HindII GTYRAC 1 cut(s) 2950
HindIII AAGCTT 3 cut(s) 772, 984, 1087
HpaII CCGG 5 cut(s) 510, 616, 1022, 2233, 2996
HphI GGTGA 4 cut(s) 293, 1037, 1867, 2497
Hpy166II GTNNAC 7 cut(s) 814, 1130, 1212, 1275, 1521, 2582, 2950
Hpy8I GTNNAC 7 cut(s) 814, 1130, 1212, 1275, 1521, 2582, 2950
Hpy99I CGWCG 1 cut(s) 2214
HpyCH4IV ACGT 2 cut(s) 350, 845
HpyF10VI GCNNNNNNNGC 5 cut(s) 1151, 1160, 2271, 3088, 3149
HpyF3I CTNAG 7 cut(s) 240, 519, 2159, 2745, 2837, 3033, 3153
HpySE526I ACGT 2 cut(s) 350, 845
HspAI GCGC 2 cut(s) 1768, 2384
Kpn2I TCCGGA 1 cut(s) 2232
Ksp22I TGATCA 1 cut(s) 697
LguI GCTCTTC 1 cut(s) 2393
LmnI GCTCC 5 cut(s) 36, 292, 857, 1854, 2911
Lsp1109I GCAGC 6 cut(s) 1047, 1265, 1297, 1525, 1858, 1952
LweI GCATC 5 cut(s) 274, 1085, 1425, 2208, 2365
MaeI CTAG 9 cut(s) 1199, 1463, 1958, 2333, 2586, 2670, 2709, 2750, 3183
MaeII ACGT 2 cut(s) 350, 845
MaeIII GTNAC 5 cut(s) 130, 970, 2368, 2998, 3047
MbiI CCGCTC 1 cut(s) 2836
MflI RGATCY 4 cut(s) 1226, 1705, 1992, 2011
MhlI GDGCHC 4 cut(s) 69, 246, 397, 2908
MlyI GAGTC 5 cut(s) 1688, 1804, 2236, 2402, 3061
MmeI TCCRAC 3 cut(s) 1555, 2114, 2430
MroI TCCGGA 1 cut(s) 2232
MroXI GAANNNNTTC 3 cut(s) 1490, 1632, 1892
MslI CAYNNNNRTG 2 cut(s) 2074, 2889
MspA1I CMGCKG 4 cut(s) 1175, 1256, 1538, 2191
MspI CCGG 5 cut(s) 510, 616, 1022, 2233, 2996
MspR9I CCNGG 8 cut(s) 93, 510, 616, 617, 640, 1022, 1023, 2252
Mva1269I GAATGC 1 cut(s) 2358
MvaI CCWGG 3 cut(s) 93, 640, 2252
MwoI GCNNNNNNNGC 5 cut(s) 1151, 1160, 2271, 3088, 3149
NciI CCSGG 5 cut(s) 510, 616, 617, 1022, 1023
NcoI CCATGG 1 cut(s) 3138
NlaIV GGNNCC 3 cut(s) 1047, 1756, 2453
NmuCI GTSAC 1 cut(s) 2368
NspI RCATGY 4 cut(s) 18, 885, 1943, 2320
NspV TTCGAA 1 cut(s) 99
PasI CCCWGGG 1 cut(s) 639
PceI AGGCCT 1 cut(s) 1565
PciI ACATGT 2 cut(s) 1939, 2316
PciSI GCTCTTC 1 cut(s) 2393
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PctI GAATGC 1 cut(s) 2358
PdmI GAANNNNTTC 3 cut(s) 1490, 1632, 1892
Pfl23II CGTACG 1 cut(s) 347
PinAI ACCGGT 1 cut(s) 2995
PkrI GCNGC 8 cut(s) 1037, 1144, 1219, 1255, 1287, 1540, 1848, 1967
PleI GAGTC 5 cut(s) 1687, 1804, 2236, 2402, 3061
PpsI GAGTC 5 cut(s) 1687, 1804, 2236, 2402, 3061
PpuMI RGGWCCY 1 cut(s) 941
PscI ACATGT 2 cut(s) 1939, 2316
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 2 cut(s) 246, 2908
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 3 cut(s) 91, 638, 2250
PspFI CCCAGC 2 cut(s) 1042, 2021
PspGI CCWGG 3 cut(s) 91, 638, 2250
PspLI CGTACG 1 cut(s) 347
PspN4I GGNNCC 3 cut(s) 1047, 1756, 2453
PspPI GGNCC 4 cut(s) 507, 941, 1046, 1754
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 2 cut(s) 1801, 1970
PsuI RGATCY 4 cut(s) 1226, 1705, 1992, 2011
PvuII CAGCTG 2 cut(s) 1175, 1538
RseI CAYNNNNRTG 2 cut(s) 2074, 2889
SacI GAGCTC 2 cut(s) 246, 2908
SapI GCTCTTC 1 cut(s) 2393
SatI GCNGC 8 cut(s) 1036, 1143, 1218, 1254, 1286, 1539, 1847, 1966
Sau96I GGNCC 4 cut(s) 507, 941, 1046, 1754
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 5 cut(s) 1688, 1804, 2236, 2402, 3061
ScrFI CCNGG 8 cut(s) 93, 510, 616, 617, 640, 1022, 1023, 2252
SduI GDGCHC 4 cut(s) 69, 246, 397, 2908
SfaNI GCATC 5 cut(s) 274, 1085, 1425, 2208, 2365
SfcI CTRYAG 5 cut(s) 1473, 1513, 1797, 1966, 2698
SfuI TTCGAA 1 cut(s) 99
SinI GGWCC 3 cut(s) 941, 1046, 1754
SmaI CCCGGG 2 cut(s) 617, 1023
SmiMI CAYNNNNRTG 2 cut(s) 2074, 2889
SmlI CTYRAG 3 cut(s) 2015, 2435, 2567
SmoI CTYRAG 3 cut(s) 2015, 2435, 2567
SseBI AGGCCT 1 cut(s) 1565
SsiI CCGC 9 cut(s) 456, 467, 840, 1143, 1196, 1217, 1256, 2191, 2834
SspMI CTAG 9 cut(s) 1199, 1463, 1958, 2333, 2586, 2670, 2709, 2750, 3183
SstI GAGCTC 2 cut(s) 246, 2908
StuI AGGCCT 1 cut(s) 1565
StyD4I CCNGG 8 cut(s) 91, 508, 614, 615, 638, 1020, 1021, 2250
StyI CCWWGG 4 cut(s) 538, 757, 1306, 3138
TaiI ACGT 2 cut(s) 353, 848
TaqII GACCGA 2 cut(s) 1742, 2753
TatI WGTACW 5 cut(s) 599, 798, 1356, 2147, 2178
TauI GCSGC 2 cut(s) 1145, 1220
TseFI GTSAC 1 cut(s) 2368
TseI GCWGC 6 cut(s) 1035, 1253, 1285, 1538, 1846, 1965
Tsp45I GTSAC 1 cut(s) 2368
TspDTI ATGAA 9 cut(s) 20, 171, 357, 678, 707, 810, 1871, 2139, 2645
TspGWI ACGGA 2 cut(s) 879, 2095
TspMI CCCGGG 2 cut(s) 615, 1021
VpaK11BI GGWCC 3 cut(s) 941, 1046, 1754
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 8 cut(s) 101, 222, 314, 494, 778, 1104, 1603, 2716
XceI RCATGY 4 cut(s) 18, 885, 1943, 2320
XmaI CCCGGG 2 cut(s) 615, 1021
XmiI GTMKAC 2 cut(s) 1211, 1274
XmnI GAANNNNTTC 3 cut(s) 1490, 1632, 1892
XspI CTAG 9 cut(s) 1199, 1463, 1958, 2333, 2586, 2670, 2709, 2750, 3183
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.