Rw2G031750

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009628
Physical Location & Seq
Forward (+)
52003261 .. 52007105
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031750.1

Sequence Viewer

Length: 3201 bp
ATGAAGATTGCATCTTTGGTTATTGTTTGGATTGTGTATGGGTTGTTGGGTTTGGGGTTCACATATTCATACCCTACTTCAGCATTTGATGATCTCAGAAATGAAAGAAGTGAAACCACCGTCACTTATAGCTATGATAGAATGGATGAAGTGAAGAAAGCATGTGGGTTTGTTCTATCTTCTGCTTCAGAACTGAAAGCTGAAGATGAGAGAGTATATACTATGAAGAAGGAGCTCTTTTTTGTGAATGGGGATTGGAGGCAGGAGGTGGGTAAGGATCCAATCATGCCATTTGATGATAGAGAAGTTCAGAGTGAGTTTTTGGGTAATAGAACTCCGTCGAACTTGGCTTCGTTTTGGCTTATGGATATTGATCGTGCCCATCGATCCAAGAAGTCACTAAGTGTTAGTGGATTCATGGTTATGGGTATTACTATAGATGGTAGCTTTATGGATTATGGGTATCAAGGGAATCCCGAGTTCCAAATGTGGCCTAGCCATTCTCAAATTACAATTTCCTTCCAAGGGATTTACACTGAAACCAAGAAGAATGGTGGCGAAAGAGTGATGTGCTTGTTGGGGAGTACAATGTTGCCGTCTCGGGAGCCTGATTCTGCTAATCCATGGGAGTGGCTGAAGGCTTCTGATTCAAGTAATCAACCTCCTCTTTCTCAAGATGATCAGATTCTACTTGTTCTTCACTTTCCGATGACTTTCAATTTGACAAATAGGGCTATCCGAGGGGAAATGAGAAGTTTAAATCCAAAGTCAAACTCAAAGTACTTTGATGAGGTTCACATTTTATCACAGTTGGGGAAGTCTGCAGTGTATAAATTTGGATCAGAAAAGATTGTTTCTAGAGCATGTGATCCATACCCTTCTAATGATAGCTTGGTATATGGTGGCATTAGCAACTACAAAGGTTATGCTATTTGCGAAATCCTTGATGAAGTTGCACAGGACCAAGCTTTCACTGTTGTGCCGAACTGGAGATGCAATGGCACCGATGAGTTTTGCAGTAAGTTAGGTCCATTTGTGACAGATAAAGAGATTAAGGCGTCAGACGGAAGCTTTAAAGGTGTTAAGCTTTACATGCAGGAAATCAAGTGTGAACAAAAAGCTGCCAGGGGAAATGCCAGTTCTGCAAGGGTTTCAGCTGTATTTAGAGCTGTTTCTCCGATGGAGAATCTATATACTGCAGCAAAGAGATCCGGGCTTAACAATATGACTGTTGCTGCTGAGGGAATTTGGAAATCTACCAGTGGACAGCTTTGCATGGTTGGTTGCCTTGGACTTGTTGATGTTGTAGGGAGTCAATGCAATACTCGGATATGTATGTATGTACCTACCTCTTTCTCCATAAAGCAACGAAGCATTCTTTATGGAAGTTTCTCTAGCATCAATGATAGTGGTACATCATATTTTCCTCTCTCCTTTGAAAAGTTAGTGCAGCCTTCGGAACTGTGGAATTATTTCAGAGTCTCCAGTCCAAACTACAAGTACACAAAAATCTATTCAGCTGCTGTTGTCTTAGAAAAAAACGAGCCCTTCAGTGTTGGAACTGTGATCAAGAAATCTTTTCTGAGTTTTCCCAAACTGGAAGACGCAGAAGCTTTTGAACTCAGTCTTTCTGTTCTCTCCGAAGATCTAACCCTTCATGTGTCTGCATTTCCCGATCCAATTCCGAAGCTTCAGCCTCCAAGAGTAGACCTTCAGATGGAGATTCTCTCAGTGGGTCCCTTGTTTGGACGCTATTGGTCTCCACAAAATGGATCCATTGCACAAGAGGAGACTCCTTACCACACAAAGTCTGAATACACTGAAAAGCAGCTCCTTCTAAATGTATCAGCACAGCTCAACATCGCCGGAAACGCCTATAGCAATCTTTCTGTGCTATATTTGGAAGGCCTTTATGATCCACATGTTGGAAAGATGTATCTAGTTGGATGCAGGGATGTTCGAGCCTCGTGGAAGATCTTGTATGATAGCATGGATCTTGAAGCCGGGCTAGATTGTTTGGTTGAGGTGGTTGTGGCTTATCCTCCCACTACATCTCGATGGTTAGTCAATCCAGCAGCCTATATTTCTGTAGCCAGCCAAAGGACTGAAGATGATCCTCTTTACTTCAGTACAGTCAAGCTCCAAACTCTTCCAATTATGTACAGGAAGCAGCGGGAAGACATTCTCTCTCGTCGGGGGATTGAGGGAATCCTCCGAGTTTTGACTCTTTCACTAGCAATATGTGGAATTTTAAGCCAACTGTTTTACATCAGGTATAATGTGGATTCTGTTCCTTACATGTCTCTAGTCATGCTCAGTATTCAAGCCATTGGGTATAGCATTCCTCTGGTCACAGGCGCTGAAGCACTCTTCAAGAAGTTAGCTTCTGAATCCTATGAAACATCAACATATGACCTTGATAACAGTCGGTGGATTCATATACTTGATTATACGGTTAAGCTTCTTCTGATGGCCTCATTGTTACTGACTTTAAGACTTTGTCAGAAAGTGTGGAAATCTCGCATCAGGTTACTTGCACAAACTCCTCTTGAACCACATCGTGTACCTAATGATAAGCGGGTGCTTATAACCACCTCAGCAATACATTTGATTGGCTATGTAATGGTTCTAGTCATTCATAGTATGAGGACCGGCCAAAGATCTATCCGGACGAAAAGCTATAAAATTGCCAGGGAAGAATCTCGTGGACTGGGGGAATGGGAAACTGAACTTGAGGAGTATGTGGGACTTGTCCAAGATTTTTTCCTACTCCCCCAGATGATTGGTAACCTGGTGTGGCAGATTGATTGTAAACCACTCAGAAAATTTTATTTCGTTGGGATCACACTGGTCAGACTCTTTCCCCACATTTATGATTATGTTAGAGCTCCGTCTCTCAACCCTTATTTTGCCGAGGAGTATGAGTTTGTCAACCCAAGTTCGGACTTTTACTCCAAGTTTGGGGATATCGCCATACCTATTACAGCAATTCTCCTTGCAGTTGTAGTCTATGTTCAGCAGAGGTGGAATTATGAGAGGCTGAGCAAGATGCTTACTTTCGGGCAATGTAGGCTTCTTCCTTTGGGTTCCAGAATGTATGAGAGGTTGCCTTCCAGTTCCAAGGCATTCGAAGCTGAGCTTGTTTCTGGTGTCAATGGTAATGCTAGACAAGAGAACAAAGAGAGTGATGATGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1066

Amino Acids

120.59

Weight (kDa)

6.19

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 51 - 266 1e-34 DUF2921 lipocalin-like domain
DUF2921_N PF25333 285 - 475 2.2e-42 DUF2921 lipocalin-like domain
DUF2921_N PF25333 515 - 712 1.7e-34 DUF2921 lipocalin-like domain
DUF2921 PF11145 724 - 1011 2.8e-59 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2588
AccB1I GGYRCC 1 cut(s) 1001
AccB7I CCANNNNNTGG 2 cut(s) 1769, 1925
AccI GTMKAC 1 cut(s) 1707
AccIII TCCGGA 1 cut(s) 2667
AciI CCGC 2 cut(s) 2173, 2578
AcoI YGGCCR 1 cut(s) 2653
AcsI RAATTY 4 cut(s) 833, 1245, 2247, 2825
AcyI GRCGYC 1 cut(s) 1058
AdeI CACNNNGTG 2 cut(s) 404, 2561
AfaI GTAC 8 cut(s) 586, 782, 1344, 1414, 1502, 2131, 2162, 2565
AfiI CCNNNNNNNGG 8 cut(s) 525, 1717, 1745, 1769, 1925, 2100, 2941, 2961
AflIII ACRYGT 2 cut(s) 1921, 2298
AgsI TTSAA 8 cut(s) 651, 718, 1439, 1619, 2000, 2324, 2374, 2552
AjnI CCWGG 3 cut(s) 1124, 2690, 2790
AleI CACNNNNGTG 1 cut(s) 977
Alw21I GWGCWC 2 cut(s) 237, 2890
Alw26I GTCTC 6 cut(s) 603, 1486, 1764, 1784, 2307, 2898
AlwNI CAGNNNCTG 2 cut(s) 1523, 2360
Ama87I CYCGRG 2 cut(s) 476, 600
Aor13HI TCCGGA 1 cut(s) 2667
AoxI GGCC 4 cut(s) 491, 1906, 2472, 2653
ApeKI GCWGC 8 cut(s) 1121, 1199, 1235, 1450, 1520, 1828, 2075, 2170
ApoI RAATTY 4 cut(s) 833, 1245, 2247, 2825
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
Asp700I GAANNNNTTC 2 cut(s) 1472, 2181
AspLEI GCGC 1 cut(s) 2360
AspS9I GGNCC 4 cut(s) 961, 1028, 1736, 2649
AsuC2I CCSGG 2 cut(s) 1213, 2005
AsuII TTCGAA 1 cut(s) 3129
AvaI CYCGRG 2 cut(s) 476, 600
AvaII GGWCC 4 cut(s) 961, 1028, 1736, 2649
BaeGI GKGCMC 1 cut(s) 382
BamHI GGATCC 2 cut(s) 277, 1772
BanI GGYRCC 1 cut(s) 1001
BanII GRGCYC 3 cut(s) 237, 1548, 2890
BauI CACGAG 2 cut(s) 1966, 2703
BbsI GAAGAC 2 cut(s) 1608, 2184
Bbv12I GWGCWC 2 cut(s) 237, 2890
BbvCI CCTCAGC 2 cut(s) 1239, 2596
BbvI GCAGC 8 cut(s) 1108, 1211, 1222, 1462, 1507, 1840, 2087, 2182
BccI CCATC 6 cut(s) 390, 434, 1174, 1711, 2052, 2464
BceAI ACGGC 1 cut(s) 580
BciT130I CCWGG 3 cut(s) 1126, 2692, 2792
BclI TGATCA 2 cut(s) 679, 1566
BcnI CCSGG 2 cut(s) 1213, 2005
BcoDI GTCTC 6 cut(s) 603, 1486, 1764, 1784, 2307, 2898
BfaI CTAG 9 cut(s) 495, 858, 1395, 1940, 2009, 2234, 2306, 2630, 3165
BfmI CTRYAG 5 cut(s) 435, 822, 1197, 1876, 2088
BfoI RGCGCY 1 cut(s) 2361
BglII AGATCT 3 cut(s) 1645, 1974, 2660
BisI GCNGC 8 cut(s) 1122, 1200, 1236, 1451, 1521, 1829, 2076, 2171
BlpI GCTNAGC 2 cut(s) 3041, 3135
BlsI GCNGC 8 cut(s) 1123, 1201, 1237, 1452, 1522, 1830, 2077, 2172
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 5 cut(s) 1126, 1213, 2005, 2692, 2792
Bme18I GGWCC 4 cut(s) 961, 1028, 1736, 2649
BmeT110I CYCGRG 2 cut(s) 476, 600
BmgT120I GGNCC 4 cut(s) 961, 1028, 1736, 2649
BmiI GGNNCC 7 cut(s) 279, 606, 1003, 1737, 1738, 1774, 3088
BmrFI CCNGG 5 cut(s) 1126, 1213, 2005, 2692, 2792
BmrI ACTGGG 1 cut(s) 2720
BmsI GCATC 6 cut(s) 20, 983, 1407, 1937, 2532, 3039
BmuI ACTGGG 1 cut(s) 2720
BpiI GAAGAC 2 cut(s) 1608, 2184
BplI GAGNNNNNCTC 2 cut(s) 1712, 1744
BpmI CTGGAG 2 cut(s) 1009, 1468
Bpu10I CCTNAGC 2 cut(s) 1239, 2596
Bpu1102I GCTNAGC 2 cut(s) 3041, 3135
Bpu14I TTCGAA 1 cut(s) 3129
BpuEI CTTGAG 2 cut(s) 657, 2753
BpuMI CCSGG 2 cut(s) 1213, 2005
Bsa29I ATCGAT 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 1058
BsaI GGTCTC 1 cut(s) 1764
BsaJI CCNNGG 8 cut(s) 523, 623, 739, 1125, 1288, 2691, 2913, 3120
BsaWI WCCGGW 1 cut(s) 2667
BsaXI ACNNNNNCTCC 4 cut(s) 2909, 2936, 2939, 2966
Bsc4I CCNNNNNNNGG 8 cut(s) 525, 1717, 1745, 1769, 1925, 2100, 2941, 2961
Bse118I RCCGGY 1 cut(s) 2651
Bse1I ACTGG 8 cut(s) 992, 1137, 1260, 1485, 1602, 2715, 2853, 3114
Bse3DI GCAATG 3 cut(s) 1003, 1776, 3071
Bse8I GATNNNNATC 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 2667
BseBI CCWGG 3 cut(s) 1126, 2692, 2792
BseCI ATCGAT 1 cut(s) 385
BseDI CCNNGG 8 cut(s) 523, 623, 739, 1125, 1288, 2691, 2913, 3120
BseGI GGATG 3 cut(s) 151, 1952, 1960
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 8 cut(s) 525, 1717, 1745, 1769, 1925, 2100, 2941, 2961
BseMI GCAATG 3 cut(s) 1003, 1776, 3071
BseNI ACTGG 8 cut(s) 992, 1137, 1260, 1485, 1602, 2715, 2853, 3114
BseRI GAGGAG 5 cut(s) 654, 1802, 2535, 2750, 2930
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 8 cut(s) 1108, 1211, 1222, 1462, 1507, 1840, 2087, 2182
BsgI GTGCAG 1 cut(s) 1469
BshFI GGCC 4 cut(s) 493, 1908, 2474, 2655
BshNI GGYRCC 1 cut(s) 1001
BshVI ATCGAT 1 cut(s) 385
BsiHKAI GWGCWC 2 cut(s) 237, 2890
BsiHKCI CYCGRG 2 cut(s) 476, 600
BsiSI CCGG 5 cut(s) 1212, 1866, 2004, 2652, 2668
BslFI GGGAC 2 cut(s) 1722, 2760
BslI CCNNNNNNNGG 8 cut(s) 525, 1717, 1745, 1769, 1925, 2100, 2941, 2961
BsmAI GTCTC 6 cut(s) 603, 1486, 1764, 1784, 2307, 2898
BsmBI CGTCTC 2 cut(s) 603, 2898
BsmFI GGGAC 2 cut(s) 1722, 2760
BsmI GAATGC 3 cut(s) 1374, 2340, 3125
BsnI GGCC 4 cut(s) 493, 1908, 2474, 2655
Bso31I GGTCTC 1 cut(s) 1764
BsoBI CYCGRG 2 cut(s) 476, 600
Bsp119I TTCGAA 1 cut(s) 3129
Bsp1286I GDGCHC 4 cut(s) 237, 382, 1548, 2890
Bsp13I TCCGGA 1 cut(s) 2667
Bsp1407I TGTACA 1 cut(s) 2160
Bsp1720I GCTNAGC 2 cut(s) 3041, 3135
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 2 cut(s) 2173, 2578
BspANI GGCC 4 cut(s) 493, 1908, 2474, 2655
BspDI ATCGAT 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 2667
BspLI GGNNCC 7 cut(s) 279, 606, 1003, 1737, 1738, 1774, 3088
BspMAI CTGCAG 2 cut(s) 826, 1201
BspT104I TTCGAA 1 cut(s) 3129
BspT107I GGYRCC 1 cut(s) 1001
BspTNI GGTCTC 1 cut(s) 1764
BsrDI GCAATG 3 cut(s) 1003, 1776, 3071
BsrFI RCCGGY 1 cut(s) 2651
BsrGI TGTACA 1 cut(s) 2160
BsrI ACTGG 8 cut(s) 992, 1137, 1260, 1485, 1602, 2715, 2853, 3114
BssAI RCCGGY 1 cut(s) 2651
BssECI CCNNGG 8 cut(s) 523, 623, 739, 1125, 1288, 2691, 2913, 3120
BssNI GRCGYC 1 cut(s) 1058
BssSI CACGAG 2 cut(s) 1966, 2703
BssT1I CCWWGG 4 cut(s) 523, 623, 1288, 3120
Bst2BI CACGAG 2 cut(s) 1966, 2703
Bst2UI CCWGG 3 cut(s) 1126, 2692, 2792
Bst6I CTCTTC 2 cut(s) 2154, 2375
BstACI GRCGYC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 2160
BstBI TTCGAA 1 cut(s) 3129
BstC8I GCNNGC 1 cut(s) 2095
BstDSI CCRYGG 1 cut(s) 623
BstEII GGTNACC 1 cut(s) 2786
BstF5I GGATG 3 cut(s) 151, 1952, 1960
BstH2I RGCGCY 1 cut(s) 2361
BstHHI GCGC 1 cut(s) 2360
BstMAI GTCTC 6 cut(s) 603, 1486, 1764, 1784, 2307, 2898
BstMWI GCNNNNNNNGC 5 cut(s) 1093, 1142, 1871, 3070, 3131
BstNI CCWGG 3 cut(s) 1126, 2692, 2792
BstNSI RCATGY 5 cut(s) 165, 867, 1096, 1925, 2302
BstPI GGTNACC 1 cut(s) 2786
BstSCI CCNGG 5 cut(s) 1124, 1211, 2003, 2690, 2790
BstSFI CTRYAG 5 cut(s) 435, 822, 1197, 1876, 2088
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 8 cut(s) 1108, 1211, 1222, 1462, 1507, 1840, 2087, 2182
BstV2I GAAGAC 2 cut(s) 1608, 2184
BstX2I RGATCY 7 cut(s) 277, 1208, 1645, 1772, 1974, 1993, 2660
BstXI CCANNNNNNTGG 2 cut(s) 630, 2783
BstYI RGATCY 7 cut(s) 277, 1208, 1645, 1772, 1974, 1993, 2660
Bsu15I ATCGAT 1 cut(s) 385
BsuRI GGCC 4 cut(s) 493, 1908, 2474, 2655
BsuTUI ATCGAT 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 623
BtgZI GCGATG 1 cut(s) 1846
BtsCI GGATG 3 cut(s) 151, 1952, 1960
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 8 cut(s) 534, 831, 972, 1267, 1558, 1737, 1818, 2846
Cac8I GCNNGC 1 cut(s) 2095
CaiI CAGNNNCTG 2 cut(s) 1523, 2360
CfoI GCGC 1 cut(s) 2360
Cfr10I RCCGGY 1 cut(s) 2651
Cfr13I GGNCC 4 cut(s) 961, 1028, 1736, 2649
ClaI ATCGAT 1 cut(s) 385
CseI GACGC 3 cut(s) 1047, 1613, 1758
CsiI ACCWGGT 1 cut(s) 2790
Csp6I GTAC 8 cut(s) 585, 781, 1343, 1413, 1501, 2130, 2161, 2564
CspCI CAANNNNNGTGG 2 cut(s) 1390, 1425
CviQI GTAC 8 cut(s) 585, 781, 1343, 1413, 1501, 2130, 2161, 2564
DraI TTTAAA 2 cut(s) 759, 1075
DraIII CACNNNGTG 2 cut(s) 404, 2561
EaeI YGGCCR 1 cut(s) 2653
Eam1104I CTCTTC 2 cut(s) 2154, 2375
EarI CTCTTC 2 cut(s) 2154, 2375
Ecl136II GAGCTC 2 cut(s) 235, 2888
Eco130I CCWWGG 4 cut(s) 523, 623, 1288, 3120
Eco147I AGGCCT 1 cut(s) 1908
Eco24I GRGCYC 3 cut(s) 237, 1548, 2890
Eco31I GGTCTC 1 cut(s) 1764
Eco32I GATATC 1 cut(s) 2968
Eco47I GGWCC 4 cut(s) 961, 1028, 1736, 2649
Eco53kI GAGCTC 2 cut(s) 235, 2888
Eco88I CYCGRG 2 cut(s) 476, 600
Eco91I GGTNACC 1 cut(s) 2786
EcoICRI GAGCTC 2 cut(s) 235, 2888
EcoO109I RGGNCCY 1 cut(s) 1736
EcoO65I GGTNACC 1 cut(s) 2786
EcoRII CCWGG 3 cut(s) 1124, 2690, 2790
EcoRV GATATC 1 cut(s) 2968
EcoT14I CCWWGG 4 cut(s) 523, 623, 1288, 3120
EcoT38I GRGCYC 3 cut(s) 237, 1548, 2890
ErhI CCWWGG 4 cut(s) 523, 623, 1288, 3120
Esp3I CGTCTC 2 cut(s) 603, 2898
FalI AAGNNNNNCTT 4 cut(s) 221, 253, 3123, 3155
FaqI GGGAC 2 cut(s) 1722, 2760
FauI CCCGC 2 cut(s) 2166, 2571
FauNDI CATATG 1 cut(s) 2410
FbaI TGATCA 2 cut(s) 679, 1566
FblI GTMKAC 1 cut(s) 1707
Fnu4HI GCNGC 8 cut(s) 1122, 1200, 1236, 1451, 1521, 1829, 2076, 2171
FokI GGATG 3 cut(s) 158, 1959, 1967
FriOI GRGCYC 3 cut(s) 237, 1548, 2890
Fsp4HI GCNGC 8 cut(s) 1122, 1200, 1236, 1451, 1521, 1829, 2076, 2171
FspBI CTAG 9 cut(s) 495, 858, 1395, 1940, 2009, 2234, 2306, 2630, 3165
GlaI GCGC 1 cut(s) 2359
GluI GCNGC 8 cut(s) 1122, 1200, 1236, 1451, 1521, 1829, 2076, 2171
GsuI CTGGAG 2 cut(s) 1009, 1468
HaeII RGCGCY 1 cut(s) 2361
HaeIII GGCC 4 cut(s) 493, 1908, 2474, 2655
HapII CCGG 5 cut(s) 1212, 1866, 2004, 2652, 2668
HgaI GACGC 3 cut(s) 1047, 1613, 1758
HhaI GCGC 1 cut(s) 2360
Hin1I GRCGYC 1 cut(s) 1058
Hin6I GCGC 1 cut(s) 2358
HinP1I GCGC 1 cut(s) 2358
HincII GTYRAC 1 cut(s) 2932
HindII GTYRAC 1 cut(s) 2932
HindIII AAGCTT 6 cut(s) 966, 1069, 1085, 1611, 1688, 2459
HpaII CCGG 5 cut(s) 1212, 1866, 2004, 2652, 2668
Hpy99I CGWCG 2 cut(s) 343, 2196
HpyF10VI GCNNNNNNNGC 5 cut(s) 1093, 1142, 1871, 3070, 3131
Hsp92I GRCGYC 1 cut(s) 1058
HspAI GCGC 1 cut(s) 2358
KflI GGGWCCC 1 cut(s) 1736
Kpn2I TCCGGA 1 cut(s) 2667
Ksp22I TGATCA 2 cut(s) 679, 1566
LmnI GCTCC 5 cut(s) 232, 604, 1836, 2145, 2893
Lsp1109I GCAGC 8 cut(s) 1108, 1211, 1222, 1462, 1507, 1840, 2087, 2182
LweI GCATC 6 cut(s) 20, 983, 1407, 1937, 2532, 3039
MabI ACCWGGT 1 cut(s) 2790
MaeI CTAG 9 cut(s) 495, 858, 1395, 1940, 2009, 2234, 2306, 2630, 3165
MaeIII GTNAC 7 cut(s) 121, 396, 1036, 2350, 2481, 2529, 2786
MflI RGATCY 7 cut(s) 277, 1208, 1645, 1772, 1974, 1993, 2660
MhlI GDGCHC 4 cut(s) 237, 382, 1548, 2890
MlyI GAGTC 5 cut(s) 1321, 1488, 1786, 2218, 2850
MmeI TCCRAC 3 cut(s) 1537, 1906, 1924
MroI TCCGGA 1 cut(s) 2667
MroXI GAANNNNTTC 2 cut(s) 1472, 2181
MseI TTAA 8 cut(s) 758, 1053, 1074, 1083, 1218, 2252, 2457, 2492
MslI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
MspA1I CMGCKG 3 cut(s) 1157, 1520, 2173
MspI CCGG 5 cut(s) 1212, 1866, 2004, 2652, 2668
MspR9I CCNGG 5 cut(s) 1126, 1213, 2005, 2692, 2792
Mva1269I GAATGC 3 cut(s) 1374, 2340, 3125
MvaI CCWGG 3 cut(s) 1126, 2692, 2792
MwoI GCNNNNNNNGC 5 cut(s) 1093, 1142, 1871, 3070, 3131
NciI CCSGG 2 cut(s) 1213, 2005
NcoI CCATGG 1 cut(s) 623
NdeI CATATG 1 cut(s) 2410
NlaIV GGNNCC 7 cut(s) 279, 606, 1003, 1737, 1738, 1774, 3088
NmeAIII GCCGAG 1 cut(s) 2938
NmuCI GTSAC 4 cut(s) 121, 396, 1036, 2350
NspI RCATGY 5 cut(s) 165, 867, 1096, 1925, 2302
NspV TTCGAA 1 cut(s) 3129
OliI CACNNNNGTG 1 cut(s) 977
PceI AGGCCT 1 cut(s) 1908
PciI ACATGT 2 cut(s) 1921, 2298
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 3 cut(s) 1374, 2340, 3125
PdmI GAANNNNTTC 2 cut(s) 1472, 2181
PflFI GACNNNGTC 1 cut(s) 2499
PflMI CCANNNNNTGG 2 cut(s) 1769, 1925
PkrI GCNGC 8 cut(s) 1123, 1201, 1237, 1452, 1522, 1830, 2077, 2172
PleI GAGTC 5 cut(s) 1320, 1487, 1786, 2218, 2850
PpsI GAGTC 5 cut(s) 1320, 1487, 1786, 2218, 2850
PpuMI RGGWCCY 1 cut(s) 1736
PscI ACATGT 2 cut(s) 1921, 2298
PsiI TTATAA 1 cut(s) 2588
Psp124BI GAGCTC 2 cut(s) 237, 2890
Psp5II RGGWCCY 1 cut(s) 1736
Psp6I CCWGG 3 cut(s) 1124, 2690, 2790
PspEI GGTNACC 1 cut(s) 2786
PspGI CCWGG 3 cut(s) 1124, 2690, 2790
PspN4I GGNNCC 7 cut(s) 279, 606, 1003, 1737, 1738, 1774, 3088
PspPI GGNCC 4 cut(s) 961, 1028, 1736, 2649
PspPPI RGGWCCY 1 cut(s) 1736
PsrI GAACNNNNNNTAC 2 cut(s) 2997, 3029
PstI CTGCAG 2 cut(s) 826, 1201
PstNI CAGNNNCTG 2 cut(s) 1523, 2360
PsuI RGATCY 7 cut(s) 277, 1208, 1645, 1772, 1974, 1993, 2660
PsyI GACNNNGTC 1 cut(s) 2499
PvuII CAGCTG 2 cut(s) 1157, 1520
RsaI GTAC 8 cut(s) 586, 782, 1344, 1414, 1502, 2131, 2162, 2565
RsaNI GTAC 8 cut(s) 585, 781, 1343, 1413, 1501, 2130, 2161, 2564
RseI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
SacI GAGCTC 2 cut(s) 237, 2890
SaqAI TTAA 8 cut(s) 758, 1053, 1074, 1083, 1218, 2252, 2457, 2492
SatI GCNGC 8 cut(s) 1122, 1200, 1236, 1451, 1521, 1829, 2076, 2171
Sau96I GGNCC 4 cut(s) 961, 1028, 1736, 2649
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 5 cut(s) 1321, 1488, 1786, 2218, 2850
ScrFI CCNGG 5 cut(s) 1126, 1213, 2005, 2692, 2792
SduI GDGCHC 4 cut(s) 237, 382, 1548, 2890
SexAI ACCWGGT 1 cut(s) 2790
SfaNI GCATC 6 cut(s) 20, 983, 1407, 1937, 2532, 3039
SfcI CTRYAG 5 cut(s) 435, 822, 1197, 1876, 2088
SfuI TTCGAA 1 cut(s) 3129
SinI GGWCC 4 cut(s) 961, 1028, 1736, 2649
SmiMI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
SmlI CTYRAG 2 cut(s) 672, 2732
SmoI CTYRAG 2 cut(s) 672, 2732
SseBI AGGCCT 1 cut(s) 1908
SsiI CCGC 2 cut(s) 2173, 2578
SspMI CTAG 9 cut(s) 495, 858, 1395, 1940, 2009, 2234, 2306, 2630, 3165
SstI GAGCTC 2 cut(s) 237, 2890
StuI AGGCCT 1 cut(s) 1908
StyD4I CCNGG 5 cut(s) 1124, 1211, 2003, 2690, 2790
StyI CCWWGG 4 cut(s) 523, 623, 1288, 3120
TaqI TCGA 5 cut(s) 341, 385, 1960, 2056, 3129
TatI WGTACW 5 cut(s) 584, 780, 1500, 2129, 2160
Tru1I TTAA 8 cut(s) 758, 1053, 1074, 1083, 1218, 2252, 2457, 2492
Tru9I TTAA 8 cut(s) 758, 1053, 1074, 1083, 1218, 2252, 2457, 2492
TscAI CASTG 8 cut(s) 541, 831, 979, 1267, 1558, 1737, 1825, 2853
TseFI GTSAC 4 cut(s) 121, 396, 1036, 2350
TseI GCWGC 8 cut(s) 1121, 1199, 1235, 1450, 1520, 1828, 2075, 2170
Tsp45I GTSAC 4 cut(s) 121, 396, 1036, 2350
TspGWI ACGGA 3 cut(s) 327, 1080, 2880
TspRI CASTG 8 cut(s) 541, 831, 979, 1267, 1558, 1737, 1825, 2853
Tth111I GACNNNGTC 1 cut(s) 2499
Van91I CCANNNNNTGG 2 cut(s) 1769, 1925
VpaK11BI GGWCC 4 cut(s) 961, 1028, 1736, 2649
XapI RAATTY 4 cut(s) 833, 1245, 2247, 2825
XbaI TCTAGA 1 cut(s) 857
XceI RCATGY 5 cut(s) 165, 867, 1096, 1925, 2302
XmiI GTMKAC 1 cut(s) 1707
XmnI GAANNNNTTC 2 cut(s) 1472, 2181
XspI CTAG 9 cut(s) 495, 858, 1395, 1940, 2009, 2234, 2306, 2630, 3165
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.