Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0006710

Physical Location & Seq

Genomic Coordinates Forward (+)

24350373 .. 24364420

Gene Structure 14048 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g31180.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3138 bp

ATGCCGACTTACGATATCAGAGGCATCGATGTCGATTTCCCATTCGAGGCCTACGATTGCCAGCTCGTTTACATGGAGAAAGTCATCCAGTCTCTCCAGCAAAGATCTAATGCACTGCTGGAGAGTCCGACTGGGACTGGGAAAACCCTTTGTCTTCTCTGTGCCACATTGGCTTGGAGGAAGAGCTTGGGGGTTTTCTCAACTGGTCCTAATGTGAAGACGAGTCTGATCAATGGTGATAAATCAGGTGGTTCGTTGTCCCAGTCCCAGTCCCAGACCAATGATCATCCTACCATAATATATGCGTCCCGCACTCACAGCCAGATCCGGCAAGTAATTCAGGAGTTGAAACGAAGCTCTTATAGGCCAAAAATGGTAGTATTGGGGTCTCGAGAGCAACTATGCATTCATGAAGACGTGAGTTTACTCCGTGGGAAAGCACAGACGAACGCCTGCCATTTCCTCTGCAGAAAGCGTACAAAGCGTCACTGCAAACATTATTCTCGTACTAATGATTACTTGAAGAATAATTCTCATCTTGGAGATGAACCTATTGACATCGAGGATTTGGTCAATATTGGAATAAAATCTGGGCCGTGCCCTTATTACTTATCCCGGGATCTTCATAAGATTGTCGATATATTTTTTGCACCCTACAACTATCTTATTGATCGTGGGTTTAGAAAATCACTACAAATTAATTGGAAAAACAGCATTCTCATATTTGATGAAGCTCACAACCTGGAAAGCATATGTGCAGATGCAGCTTCTTTTGACTTGCCATCTTGGCTTCTTACAGCATGCATTTCTGAAGCGAAAAATTGTATTGACCTTTCCGTACAAAGAAGGGATGAATCAACCGATAAATCAAAGAATCCAGATAACTTTGCAATTCTTAGAGCACTTCTTTTGAAGCTTGAGAAGCGGATTTCTGAAGTGCCTATTCAATCGAAGGAATTGGGATACACTAAGCCTGGAGCGTATATCTATGAACTACTTGCCGATTTAAACATCACATCAGATACTGTCCCTATGATCATTAATATAATTGAGGAAGCTACTCTACTTATTGAGGAAGATAACCAGCAGAATTCAAAACGTAATGTCTGCCGGCTAGAGATCATTAGTGACATCTTGAATACAGTTTTCAGAGATATAAGCAATGCGAAATCGTATCGTGTTCACGTACAGGAAGTTCAAGGAAGTGCAACAGATGGTCTGAAGGGTAAGCCATCTAGGACACTCAGCTGGTGGTGCTTTCATCCTGGAATTGCAATGGAGGAATTCTCCAAGATGGATGTGGGCTCTATTATATTGACATCTGGCACACTATCTCCTTTAGAATCATTTGCACAGGAGCTAAAACTAGAATTTCCTGTGCGGCTGGAAAATCCTCATGTTATCACCCCGAATCAGATATGGGCTGGAGTTGTTCCAGCTGGCCCTTCCGGTTGCTCTTTCAACTCCTCTTATAGAACACGTGACTCAATTGAGTATAAGCAGGATCTTGGAAATGCTATTGTCAATTTTGCTCGAATTGTACCTGATGGATTGCTTGTATTCTTTCCTTCTTACTACCTTTTGGATCAATGCATTGCGTGCTGGAAGAATATGAGCCAGGCCAATTCAACTACAATATGGGAAAGAATATGCAAACACAAGAAACCTGTTGTAGAACCTAGACAATCTTCATTATTTCTTTCATCAATTGAGGACTATATGACTAAGCTAAAGGACACCTCAGCTTCTGGTGCAATATTTTTTGCCGTTTGCCGTGGTAAGGTGAGTGAAGGATTAGATTTTGCCGATCACGCTGGAAGAGCTGTAATTGTTACTGGTATGCCGTTTGCCACATGGAATGATCCTAAGGTTCGTCTGAAACGTGAGTACTTAGATGAACAGGCATGTTCTCAACAGAAAGTATGTAAGGTGCAAGTTCTGACTGGAGAAGACTGGTACAACCAACAAGCATCACGAGCTGTGAATCAGGCTGTTGGACGTGTAATCCGTCATCGTCATGATTATGGAGCAATTATTTTTCTTGATGAAAGGTTTGCACAGCCAAATCGTCAATCTCAAGTATCTCTTTGGATAAGGCCTCATATCAAGTGCTACTCCAAATTTGGGGAAGTGGTTTCTTCGTTGACCCGTTTCTTCCGGGATGAAAAAGCTCGGGGTCCTACAAAACTGAATCTATTAACAACTGAAAAAGAAGGAAAGATATGTGAGTCGCAAATTCAGCAACCTTTAGACAAATCTTATCTGGATAAATGTTTCCCTGAGAATACACAAGTGGGCCAAGACTGTCCTGTAAAGTTATCTCCTCTTCCTGAAGCTAGAAGGAAAAAGGTGTCAAGCCTGTTGGGTGAAATCGTCCCTGCTAATCGCTCATCTGTTACTTGCTGCAAGGATCAGTCTGTGTCATGGAAGTGCTCAAGTACCCTATTAGGAAATGAGAAAAAGCCTCTTACCCTTAAGAGAGAGAGTATGCAGTATCAAGATAGAAACATAATTGATCTTACCCTTAAGAGGGACAGTATGCAGTATCAAGATAGAAACATAATTGATCTTACTGGTGGATCAATGTTAAAAGAGAAGTGGAGTGAAGAAATGATAACACTGTGTTCTGCAAAGAAGTGTAAAGTAAGTGCAGAGCATGATGCAGCGCAACATATTAACAATTCTCCTGAGCATTCATCTCATACAATGGATTTCGAATCACATGGCCTTAATTCAATCAGAAGTGAGAGCTCACATAGATCTAAGTCATCTGATAGAGAAAGTACACAAAATGCTCAAGGTGGTTCAAAACTGTTGCATAAAGATAATGATCCTGTGCCTTCTGGTGATGAGGAAACGAGAGGATCTCAATTTCTTATTCAGGTTAAAGAGAAACTTAGTGCTTTGGAGTATAAAGAATTTGTAAATTTGATGAAGGCACTGAAGTCCAAAGCAATGAACATAAGTGAAGTTTTGCAATCAATTGCTAGATTGTTTTGTGGACCAGAGAGGCTTCCCCTTCTTAAAAGATTCAAGTACTTCATTCCTGCAAAGTATCATTCCATGTATGATCACTATTTTCAAACCAATGGATCTTCTCAGAGTGAGATGCATGATAACAAGGCCGGTTTTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000018 GO:0000723 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009555 GO:0009892 GO:0009987 GO:0010569 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031324 GO:0032200 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0036297 GO:0042592 GO:0043007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048229 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070716 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0099402 GO:1901360 GO:1901576 GO:2000779 GO:2001020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1046

Amino Acids

118.33

Weight (kDa)

8.57

Isoelectric Point (pI)

49.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	101 - 261	4.5e-53	DEAD_2
ARCH_RTEL1	PF23109	267 - 422	2.9e-40	RTEL1 helicase, ARCH domain
Helicase_C_2	PF13307	507 - 667	4.5e-43	Helicase C-terminal domain
HHD_RTEL1	PF23116	880 - 994	7.9e-13	RTEL1, HHD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950
fragaria_vesca	FvH4_6g31180 FvH4_6g31180 FvH4_6g31180 FvH4_6g31180
malus_domestica	MD17G1195000.v1.1
prunus_persica	Prupe.3G071000_v2.0.a1 Prupe.3G071000_v2.0.a1 Prupe.3G071000_v2.0.a1
pyrus_communis	pycom17g20090
rosa_chinensis	RchiOBHm_Chr2g0137871
rosa_laevigata	RLG00000019686
rosa_multiflora	Rmu_sc0002170.1_g000014
rosa_roxburghii	Rroxscaffold_2G00106700
rosa_rugosa	Rorug02G0347600
rosa_samantha	Rh2AG393900 Rh2BG401300 Rh2CG380600 Rh2DG416100
rosa_wichuraiana	Rw2G032240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	310, 925, 1381
AcsI	RAATTY	7 cut(s)	1090, 1283, 1370, 2120, 2235, 2918, 2926
AcuI	CTGAAG	5 cut(s)	831, 954, 1241, 2352, 2963
AcvI	CACGTG	1 cut(s)	1481
AfiI	CCNNNNNNNGG	5 cut(s)	46, 1780, 2124, 2529, 2530
AflII	CTTAAG	2 cut(s)	2474, 2525
AflIII	ACRYGT	2 cut(s)	1478, 1999
AjiI	CACGTC	2 cut(s)	418, 2000
AjnI	CCWGG	4 cut(s)	741, 973, 1264, 1617
Alw21I	GWGCWC	3 cut(s)	904, 2435, 2753
Alw26I	GTCTC	2 cut(s)	96, 393
AlwNI	CAGNNNCTG	2 cut(s)	1025, 1748
Ama87I	CYCGRG	3 cut(s)	390, 615, 2172
AoxI	GGCC	9 cut(s)	48, 365, 593, 1441, 1620, 2096, 2296, 2725, 3123
ApeKI	GCWGC	3 cut(s)	764, 2403, 2663
ApoI	RAATTY	7 cut(s)	1090, 1283, 1370, 2120, 2235, 2918, 2926
AseI	ATTAAT	2 cut(s)	699, 1041
AspLEI	GCGC	1 cut(s)	2668
AspS9I	GGNCC	6 cut(s)	206, 593, 1442, 2177, 2296, 3002
AsuC2I	CCSGG	3 cut(s)	616, 617, 2159
AsuHPI	GGTGA	5 cut(s)	248, 1396, 1795, 2378, 2858
AsuII	TTCGAA	1 cut(s)	2715
AvaI	CYCGRG	3 cut(s)	390, 615, 2172
AvaII	GGWCC	3 cut(s)	206, 2177, 3002
AxyI	CCTNAGG	1 cut(s)	1866
BaeGI	GKGCMC	1 cut(s)	602
BanII	GRGCYC	2 cut(s)	1307, 2753
BarI	GAAGNNNNNNTAC	2 cut(s)	1047, 1079
BauI	CACGAG	1 cut(s)	1974
BbrPI	CACGTG	1 cut(s)	1481
BbsI	GAAGAC	4 cut(s)	146, 224, 420, 1956
Bbv12I	GWGCWC	3 cut(s)	904, 2435, 2753
BbvCI	CCTCAGC	1 cut(s)	1741
BbvI	GCAGC	3 cut(s)	776, 2390, 2675
BccI	CCATC	5 cut(s)	790, 1208, 1240, 1288, 1541
BceAI	ACGGC	4 cut(s)	580, 1751, 1758, 1828
BcgI	CGANNNNNNTGC	2 cut(s)	13, 47
BciT130I	CCWGG	4 cut(s)	743, 975, 1266, 1619
BciVI	GTATCC	1 cut(s)	956
BclI	TGATCA	4 cut(s)	228, 283, 1035, 3070
BcnI	CCSGG	3 cut(s)	616, 617, 2159
BcoDI	GTCTC	2 cut(s)	96, 393
BfaI	CTAG	6 cut(s)	1115, 1236, 1367, 1680, 2337, 2988
BfmI	CTRYAG	1 cut(s)	466
BfrI	CTTAAG	2 cut(s)	2474, 2525
BfuI	GTATCC	1 cut(s)	956
BglI	GCCNNNNNGGC	2 cut(s)	170, 787
BglII	AGATCT	2 cut(s)	104, 2759
BisI	GCNGC	4 cut(s)	765, 1382, 2404, 2664
BlsI	GCNGC	4 cut(s)	766, 1383, 2405, 2665
BmcAI	AGTACT	2 cut(s)	1889, 3038
Bme1390I	CCNGG	7 cut(s)	616, 617, 743, 975, 1266, 1619, 2159
Bme18I	GGWCC	3 cut(s)	206, 2177, 3002
BmeT110I	CYCGRG	3 cut(s)	390, 615, 2172
BmgBI	CACGTC	2 cut(s)	418, 2000
BmgT120I	GGNCC	6 cut(s)	206, 593, 1442, 2177, 2296, 3002
BmiI	GGNNCC	1 cut(s)	2178
BmrFI	CCNGG	7 cut(s)	616, 617, 743, 975, 1266, 1619, 2159
BmrI	ACTGGG	4 cut(s)	141, 147, 256, 262
BmsI	GCATC	5 cut(s)	33, 751, 1979, 2650, 3099
BmuI	ACTGGG	4 cut(s)	141, 147, 256, 262
BpiI	GAAGAC	4 cut(s)	146, 224, 420, 1956
BplI	GAGNNNNNCTC	4 cut(s)	1271, 1303, 2851, 2883
BpmI	CTGGAG	5 cut(s)	80, 140, 996, 1446, 1965
Bpu10I	CCTNAGC	2 cut(s)	1741, 2688
Bpu14I	TTCGAA	1 cut(s)	2715
BpuEI	CTTGAG	4 cut(s)	938, 2061, 2419, 2781
BpuMI	CCSGG	3 cut(s)	616, 617, 2159
Bsa29I	ATCGAT	1 cut(s)	27
BsaAI	YACGTR	2 cut(s)	1186, 1481
BsaBI	GATNNNNATC	1 cut(s)	2829
BsaI	GGTCTC	1 cut(s)	393
BsaJI	CCNNGG	3 cut(s)	430, 615, 1774
BsaWI	WCCGGW	1 cut(s)	1448
BsaXI	ACNNNNNCTCC	2 cut(s)	1318, 1348
Bsc4I	CCNNNNNNNGG	5 cut(s)	46, 1780, 2124, 2529, 2530
Bse118I	RCCGGY	2 cut(s)	1110, 3125
Bse21I	CCTNAGG	1 cut(s)	1866
Bse3DI	GCAATG	4 cut(s)	1168, 1281, 1593, 2961
Bse8I	GATNNNNATC	1 cut(s)	2829
BseBI	CCWGG	4 cut(s)	743, 975, 1266, 1619
BseCI	ATCGAT	1 cut(s)	27
BseDI	CCNNGG	3 cut(s)	430, 615, 1774
BseGI	GGATG	6 cut(s)	84, 286, 856, 1261, 1303, 2167
BseJI	GATNNNNATC	1 cut(s)	2829
BseLI	CCNNNNNNNGG	5 cut(s)	46, 1780, 2124, 2529, 2530
BseMI	GCAATG	4 cut(s)	1168, 1281, 1593, 2961
BseMII	CTCAG	5 cut(s)	1258, 1755, 2271, 2679, 3113
BseRI	GAGGAG	2 cut(s)	1456, 2313
BseSI	GKGCMC	1 cut(s)	602
BseXI	GCAGC	3 cut(s)	776, 2390, 2675
BsgI	GTGCAG	2 cut(s)	777, 2670
BshFI	GGCC	9 cut(s)	50, 367, 595, 1443, 1622, 2098, 2298, 2727, 3125
BshVI	ATCGAT	1 cut(s)	27
BsiHKAI	GWGCWC	3 cut(s)	904, 2435, 2753
BsiHKCI	CYCGRG	3 cut(s)	390, 615, 2172
BsiSI	CCGG	6 cut(s)	328, 616, 1111, 1449, 2158, 3126
BslFI	GGGAC	8 cut(s)	148, 244, 250, 256, 292, 1013, 2360, 2546
BslI	CCNNNNNNNGG	5 cut(s)	46, 1780, 2124, 2529, 2530
BsmAI	GTCTC	2 cut(s)	96, 393
BsmFI	GGGAC	8 cut(s)	148, 244, 250, 256, 292, 1013, 2360, 2546
BsmI	GAATGC	3 cut(s)	405, 714, 2692
BsnI	GGCC	9 cut(s)	50, 367, 595, 1443, 1622, 2098, 2298, 2727, 3125
Bso31I	GGTCTC	1 cut(s)	393
BsoBI	CYCGRG	3 cut(s)	390, 615, 2172
Bsp119I	TTCGAA	1 cut(s)	2715
Bsp1286I	GDGCHC	5 cut(s)	602, 904, 1307, 2435, 2753
BspACI	CCGC	3 cut(s)	310, 925, 1381
BspANI	GGCC	9 cut(s)	50, 367, 595, 1443, 1622, 2098, 2298, 2727, 3125
BspCNI	CTCAG	5 cut(s)	1257, 1754, 2272, 2680, 3112
BspDI	ATCGAT	1 cut(s)	27
BspHI	TCATGA	2 cut(s)	409, 2017
BspLI	GGNNCC	1 cut(s)	2178
BspMAI	CTGCAG	1 cut(s)	470
BspQI	GCTCTTC	2 cut(s)	176, 1813
BspT104I	TTCGAA	1 cut(s)	2715
BspTI	CTTAAG	2 cut(s)	2474, 2525
BspTNI	GGTCTC	1 cut(s)	393
BsrDI	GCAATG	4 cut(s)	1168, 1281, 1593, 2961
BsrFI	RCCGGY	2 cut(s)	1110, 3125
BssAI	RCCGGY	2 cut(s)	1110, 3125
BssECI	CCNNGG	3 cut(s)	430, 615, 1774
BssSI	CACGAG	1 cut(s)	1974
Bst2BI	CACGAG	1 cut(s)	1974
Bst2UI	CCWGG	4 cut(s)	743, 975, 1266, 1619
Bst4CI	ACNGT	6 cut(s)	1027, 1144, 2306, 2537, 2622, 2814
Bst6I	CTCTTC	3 cut(s)	176, 1813, 2331
BstAFI	CTTAAG	2 cut(s)	2474, 2525
BstAPI	GCANNNNNTGC	1 cut(s)	1599
BstBAI	YACGTR	2 cut(s)	1186, 1481
BstBI	TTCGAA	1 cut(s)	2715
BstC8I	GCNNGC	6 cut(s)	62, 454, 802, 1112, 1441, 1600
BstDSI	CCRYGG	2 cut(s)	430, 1774
BstF5I	GGATG	6 cut(s)	84, 286, 856, 1261, 1303, 2167
BstHHI	GCGC	1 cut(s)	2668
BstMAI	GTCTC	2 cut(s)	96, 393
BstNI	CCWGG	4 cut(s)	743, 975, 1266, 1619
BstNSI	RCATGY	2 cut(s)	804, 1908
BstSCI	CCNGG	7 cut(s)	614, 615, 741, 973, 1264, 1617, 2157
BstSFI	CTRYAG	1 cut(s)	466
BstSLI	GKGCMC	1 cut(s)	602
BstV1I	GCAGC	3 cut(s)	776, 2390, 2675
BstV2I	GAAGAC	4 cut(s)	146, 224, 420, 1956
BstX2I	RGATCY	7 cut(s)	104, 324, 619, 1504, 2759, 2864, 3092
BstYI	RGATCY	7 cut(s)	104, 324, 619, 1504, 2759, 2864, 3092
Bsu15I	ATCGAT	1 cut(s)	27
Bsu36I	CCTNAGG	1 cut(s)	1866
BsuI	GTATCC	1 cut(s)	956
BsuRI	GGCC	9 cut(s)	50, 367, 595, 1443, 1622, 2098, 2298, 2727, 3125
BsuTUI	ATCGAT	1 cut(s)	27
BtgI	CCRYGG	2 cut(s)	430, 1774
BtrI	CACGTC	2 cut(s)	418, 2000
BtsCI	GGATG	6 cut(s)	84, 286, 856, 1261, 1303, 2167
BtsI	GCAGTG	2 cut(s)	113, 487
BtsIMutI	CAGTG	4 cut(s)	113, 487, 2618, 2939
Cac8I	GCNNGC	6 cut(s)	62, 454, 802, 1112, 1441, 1600
CaiI	CAGNNNCTG	2 cut(s)	1025, 1748
CciI	TCATGA	2 cut(s)	409, 2017
CfoI	GCGC	1 cut(s)	2668
Cfr10I	RCCGGY	2 cut(s)	1110, 3125
Cfr13I	GGNCC	6 cut(s)	206, 593, 1442, 2177, 2296, 3002
Cfr9I	CCCGGG	1 cut(s)	615
ClaI	ATCGAT	1 cut(s)	27
CseI	GACGC	2 cut(s)	294, 473
DraI	TTTAAA	1 cut(s)	1008
Eam1104I	CTCTTC	3 cut(s)	176, 1813, 2331
EarI	CTCTTC	3 cut(s)	176, 1813, 2331
Ecl136II	GAGCTC	1 cut(s)	2751
Eco147I	AGGCCT	2 cut(s)	50, 2098
Eco24I	GRGCYC	2 cut(s)	1307, 2753
Eco31I	GGTCTC	1 cut(s)	393
Eco32I	GATATC	1 cut(s)	16
Eco47I	GGWCC	3 cut(s)	206, 2177, 3002
Eco53kI	GAGCTC	1 cut(s)	2751
Eco57I	CTGAAG	5 cut(s)	831, 954, 1241, 2352, 2963
Eco72I	CACGTG	1 cut(s)	1481
Eco81I	CCTNAGG	1 cut(s)	1866
Eco88I	CYCGRG	3 cut(s)	390, 615, 2172
EcoICRI	GAGCTC	1 cut(s)	2751
EcoO109I	RGGNCCY	1 cut(s)	2177
EcoRI	GAATTC	2 cut(s)	1090, 1283
EcoRII	CCWGG	4 cut(s)	741, 973, 1264, 1617
EcoRV	GATATC	1 cut(s)	16
EcoT22I	ATGCAT	4 cut(s)	407, 806, 1595, 3114
EcoT38I	GRGCYC	2 cut(s)	1307, 2753
FalI	AAGNNNNNCTT	4 cut(s)	2070, 2102, 2880, 2912
FaqI	GGGAC	8 cut(s)	148, 244, 250, 256, 292, 1013, 2360, 2546
FauI	CCCGC	1 cut(s)	317
FauNDI	CATATG	1 cut(s)	752
FbaI	TGATCA	4 cut(s)	228, 283, 1035, 3070
Fnu4HI	GCNGC	4 cut(s)	765, 1382, 2404, 2664
FokI	GGATG	6 cut(s)	71, 273, 863, 1248, 1310, 2174
FriOI	GRGCYC	2 cut(s)	1307, 2753
Fsp4HI	GCNGC	4 cut(s)	765, 1382, 2404, 2664
FspBI	CTAG	6 cut(s)	1115, 1236, 1367, 1680, 2337, 2988
GlaI	GCGC	1 cut(s)	2667
GluI	GCNGC	4 cut(s)	765, 1382, 2404, 2664
GsuI	CTGGAG	5 cut(s)	80, 140, 996, 1446, 1965
HaeIII	GGCC	9 cut(s)	50, 367, 595, 1443, 1622, 2098, 2298, 2727, 3125
HapII	CCGG	6 cut(s)	328, 616, 1111, 1449, 2158, 3126
HgaI	GACGC	2 cut(s)	294, 473
HhaI	GCGC	1 cut(s)	2668
Hin6I	GCGC	1 cut(s)	2666
HinP1I	GCGC	1 cut(s)	2666
HincII	GTYRAC	1 cut(s)	2145
HindII	GTYRAC	1 cut(s)	2145
HindIII	AAGCTT	1 cut(s)	914
HpaII	CCGG	6 cut(s)	328, 616, 1111, 1449, 2158, 3126
HphI	GGTGA	5 cut(s)	248, 1396, 1795, 2378, 2858
Hpy166II	GTNNAC	6 cut(s)	70, 425, 1183, 2145, 2786, 3002
Hpy8I	GTNNAC	6 cut(s)	70, 425, 1183, 2145, 2786, 3002
HpyCH4III	ACNGT	6 cut(s)	1027, 1144, 2306, 2537, 2622, 2814
HpyCH4IV	ACGT	6 cut(s)	417, 1099, 1185, 1480, 1882, 1999
HpySE526I	ACGT	6 cut(s)	417, 1099, 1185, 1480, 1882, 1999
HspAI	GCGC	1 cut(s)	2666
KroI	GCCGGC	1 cut(s)	1110
KroNI	GCCGGC	1 cut(s)	1112
Ksp22I	TGATCA	4 cut(s)	228, 283, 1035, 3070
LguI	GCTCTTC	2 cut(s)	176, 1813
LmnI	GCTCC	3 cut(s)	977, 1357, 2027
Lsp1109I	GCAGC	3 cut(s)	776, 2390, 2675
LweI	GCATC	5 cut(s)	33, 751, 1979, 2650, 3099
MaeI	CTAG	6 cut(s)	1115, 1236, 1367, 1680, 2337, 2988
MaeII	ACGT	6 cut(s)	417, 1099, 1185, 1480, 1882, 1999
MaeIII	GTNAC	5 cut(s)	485, 1127, 1481, 1831, 2395
MfeI	CAATTG	3 cut(s)	1488, 1707, 2982
MflI	RGATCY	7 cut(s)	104, 324, 619, 1504, 2759, 2864, 3092
MhlI	GDGCHC	5 cut(s)	602, 904, 1307, 2435, 2753
MlyI	GAGTC	4 cut(s)	133, 232, 1478, 2237
MmeI	TCCRAC	2 cut(s)	152, 1975
Mph1103I	ATGCAT	4 cut(s)	407, 806, 1595, 3114
MroNI	GCCGGC	1 cut(s)	1110
MslI	CAYNNNNRTG	3 cut(s)	2016, 2022, 2428
MspA1I	CMGCKG	2 cut(s)	1248, 1439
MspCI	CTTAAG	2 cut(s)	2474, 2525
MspI	CCGG	6 cut(s)	328, 616, 1111, 1449, 2158, 3126
MspR9I	CCNGG	7 cut(s)	616, 617, 743, 975, 1266, 1619, 2159
MunI	CAATTG	3 cut(s)	1488, 1707, 2982
Mva1269I	GAATGC	3 cut(s)	405, 714, 2692
MvaI	CCWGG	4 cut(s)	743, 975, 1266, 1619
NaeI	GCCGGC	1 cut(s)	1112
NciI	CCSGG	3 cut(s)	616, 617, 2159
NdeI	CATATG	1 cut(s)	752
NgoMIV	GCCGGC	1 cut(s)	1110
NlaIV	GGNNCC	1 cut(s)	2178
NmuCI	GTSAC	3 cut(s)	485, 1127, 1481
NsiI	ATGCAT	4 cut(s)	407, 806, 1595, 3114
NspI	RCATGY	2 cut(s)	804, 1908
NspV	TTCGAA	1 cut(s)	2715
PaeI	GCATGC	1 cut(s)	804
PaeR7I	CTCGAG	1 cut(s)	390
PagI	TCATGA	2 cut(s)	409, 2017
PceI	AGGCCT	2 cut(s)	50, 2098
PciSI	GCTCTTC	2 cut(s)	176, 1813
PcsI	WCGNNNNNNNCGW	3 cut(s)	51, 1879, 2005
PctI	GAATGC	3 cut(s)	405, 714, 2692
PdiI	GCCGGC	1 cut(s)	1112
PfeI	GAWTC	8 cut(s)	854, 874, 1343, 1411, 1984, 2191, 2717, 3030
PfoI	TCCNGGA	2 cut(s)	1264, 2157
PkrI	GCNGC	4 cut(s)	766, 1383, 2405, 2665
PleI	GAGTC	4 cut(s)	132, 231, 1478, 2236
PmaCI	CACGTG	1 cut(s)	1481
PmlI	CACGTG	1 cut(s)	1481
PpsI	GAGTC	4 cut(s)	132, 231, 1478, 2236
Ppu21I	YACGTR	2 cut(s)	1186, 1481
PpuMI	RGGWCCY	1 cut(s)	2177
PshBI	ATTAAT	2 cut(s)	699, 1041
Psp124BI	GAGCTC	1 cut(s)	2753
Psp5II	RGGWCCY	1 cut(s)	2177
Psp6I	CCWGG	4 cut(s)	741, 973, 1264, 1617
PspCI	CACGTG	1 cut(s)	1481
PspGI	CCWGG	4 cut(s)	741, 973, 1264, 1617
PspN4I	GGNNCC	1 cut(s)	2178
PspPI	GGNCC	6 cut(s)	206, 593, 1442, 2177, 2296, 3002
PspPPI	RGGWCCY	1 cut(s)	2177
PsrI	GAACNNNNNNTAC	2 cut(s)	1179, 1211
PstI	CTGCAG	1 cut(s)	470
PstNI	CAGNNNCTG	2 cut(s)	1025, 1748
PsuI	RGATCY	7 cut(s)	104, 324, 619, 1504, 2759, 2864, 3092
PvuII	CAGCTG	2 cut(s)	1248, 1439
RseI	CAYNNNNRTG	3 cut(s)	2016, 2022, 2428
SacI	GAGCTC	1 cut(s)	2753
SapI	GCTCTTC	2 cut(s)	176, 1813
SatI	GCNGC	4 cut(s)	765, 1382, 2404, 2664
Sau96I	GGNCC	6 cut(s)	206, 593, 1442, 2177, 2296, 3002
ScaI	AGTACT	2 cut(s)	1889, 3038
SchI	GAGTC	4 cut(s)	133, 232, 1478, 2237
ScrFI	CCNGG	7 cut(s)	616, 617, 743, 975, 1266, 1619, 2159
SduI	GDGCHC	5 cut(s)	602, 904, 1307, 2435, 2753
SfaNI	GCATC	5 cut(s)	33, 751, 1979, 2650, 3099
SfcI	CTRYAG	1 cut(s)	466
Sfr274I	CTCGAG	1 cut(s)	390
SfuI	TTCGAA	1 cut(s)	2715
SinI	GGWCC	3 cut(s)	206, 2177, 3002
SlaI	CTCGAG	1 cut(s)	390
SmaI	CCCGGG	1 cut(s)	617
SmiMI	CAYNNNNRTG	3 cut(s)	2016, 2022, 2428
SmlI	CTYRAG	7 cut(s)	390, 917, 2076, 2434, 2474, 2525, 2796
SmoI	CTYRAG	7 cut(s)	390, 917, 2076, 2434, 2474, 2525, 2796
SphI	GCATGC	1 cut(s)	804
SseBI	AGGCCT	2 cut(s)	50, 2098
SsiI	CCGC	3 cut(s)	310, 925, 1381
SspI	AATATT	2 cut(s)	577, 1758
SspMI	CTAG	6 cut(s)	1115, 1236, 1367, 1680, 2337, 2988
SstI	GAGCTC	1 cut(s)	2753
StuI	AGGCCT	2 cut(s)	50, 2098
StyD4I	CCNGG	7 cut(s)	614, 615, 741, 973, 1264, 1617, 2157
TaaI	ACNGT	6 cut(s)	1027, 1144, 2306, 2537, 2622, 2814
TaiI	ACGT	6 cut(s)	420, 1102, 1188, 1483, 1885, 2002
TaqI	TCGA	9 cut(s)	27, 33, 45, 391, 561, 636, 950, 1534, 2715
TatI	WGTACW	3 cut(s)	1887, 2783, 3036
TauI	GCSGC	1 cut(s)	1384
TfiI	GAWTC	8 cut(s)	854, 874, 1343, 1411, 1984, 2191, 2717, 3030
TscAI	CASTG	4 cut(s)	120, 494, 2625, 2946
TseFI	GTSAC	3 cut(s)	485, 1127, 1481
TseI	GCWGC	3 cut(s)	764, 2403, 2663
Tsp45I	GTSAC	3 cut(s)	485, 1127, 1481
TspGWI	ACGGA	3 cut(s)	419, 826, 1997
TspMI	CCCGGG	1 cut(s)	615
TspRI	CASTG	4 cut(s)	120, 494, 2625, 2946
Vha464I	CTTAAG	2 cut(s)	2474, 2525
VpaK11BI	GGWCC	3 cut(s)	206, 2177, 3002
VspI	ATTAAT	2 cut(s)	699, 1041
XapI	RAATTY	7 cut(s)	1090, 1283, 1370, 2120, 2235, 2918, 2926
XceI	RCATGY	2 cut(s)	804, 1908
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1297
XhoI	CTCGAG	1 cut(s)	390
XmaI	CCCGGG	1 cut(s)	615
XspI	CTAG	6 cut(s)	1115, 1236, 1367, 1680, 2337, 2988
ZrmI	AGTACT	2 cut(s)	1889, 3038
Zsp2I	ATGCAT	4 cut(s)	407, 806, 1595, 3114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.