Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006710

Physical Location & Seq

Genomic Coordinates Reverse (-)

23362140 .. 23378270

Gene Structure 16131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1195000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGCCGACGTACAACATCAGAGGCATCGATGTCGATTTCCCATTTGAGGCGTACGATTGCCAGCTCGTTTACATGGAGAAAGTCATTCAATCTCTCCAAGAAAAAAGTAATGCACTGCTGGAGAGTCCCACTGGAACTGGGAAGACCCTTTGTCTTCTGTGCGCCACATTGGCCTGGAGGAAGAGTTTGGGCCCTTTCTCAACTGGTCCTAATGTCAAGATGAGTGTGATCACAGGAGATGCATCAGGTGGTCCCTCGTCACAGTCTGTGACAAAAGGTTTTCCTACCATAGTATATACGTCCCGCACTCACAGCCAGATCCGGCAAGTAATTCAAGAGTTGAAAAGAAGCGCTTACAGGCCAAAAATGGTAGTGTTGGGATCTCGAGAGCAATTATGCATTGATGAGGAAGTGAGTTTACTTCGCGGAAGAACACAGACAAAGGCTTGCCATGTCCTCTGCAGAAAGCGCACAAAGGATACAAAGCGTCGTTGTTCTCATTACTCTCGTGGTTCTGGTTTCTTGAAGAGTAATCCTCATCTTGGAGATGAACCTGTTGATATCGAGGATTTGGTCAATACTGGAAAAAAATTGGGGCCGTGTCCTTATTATTTATCGAGGGAGCTCCATAAGGTTGTTGATATATTATTTGCACCTTATAACTATCTCATTGATCCTGCATTTAGAAAATCGCTACAGGTTGATTGGAAAAACAGTATTCTGATATTTGACGAAGCTCACAACCTGGAAAGCATATGTGCAGATGCTGCTTCCTTTGACTTGCCCTCTTGGCTTCTTACAACTTGCATTTCTGAAGCAAAAAATTGTATTGACCTTTCTTCAAAAAGAAGGGAAGACTCAACTGATAAATCACAGAATCCAGATGACTTTGCTATCCTCAGAGCGCTACTATTGAAGCTTGAGAAGCGTATTTCTGAAGTACCTATCGAATCCAAGGAATTGGGATACACAAAGCCTGGGCCTTATATCTATGAACTCCTGGCGGATTTAAACATCTCGCATGATACTGTCCCTAAGCTCATTGCAATACTTGAGGACGCTACTGTACTTATTGAGGAAGATAACCAGGACAAGGCGAATCGAAATGCCTGCCGGCTGGAAAGCATTAGTGACATGCTTAATATAATTTTCAGAGATAAAGACAATAATCATGCGAAATCCTATCGTGTTCATGTACAAGAAGTTGAAGCCAGTGCAATAGATGGTTTAAAGGGGAAGGCTTCTAGGACACTTAGCTGGTGGTGTTTTCATCCTGGAATTGCAATGGAAGAATTTGCCAGGATAGGTGTGGGCTCTATTATACTGACATCTGGCACATTATCTCCCTTGGATTCGTTTGCACAGGAACTGAAACTAGAGTTTCCTGTGCGGCTGGAAAATCCTCATGTTATTACCCCGAATCAGATATGGGCTGGAGTTGTACCAGCTGGCCCTTCTGGATTCTCTTTCAACTCCTCTTACAGAAATCGTGATTCTACGGAGTACAAGCAGGACCTTGGAAATGCTATTGTCAATTTTGCTCGAATTGTTCCCAATGGATTGCTAGTATTCTTTCCGTCGTACTACATTCTGGACCAATGCATTGCCTGCTGGAAGAAAATGAGCCATGCCAATTCAACTACAACATGGGAAAGAATCTCTAAACATAAGAAACCTGTTGTGGAACCTAGACAATCTTCGTTGTTTCTTTTGTCAATTGAGGATTATATGGCAAAGCTGAAGGACACCTCAGCTTCTGGTGCAGTATTTTTTGCTGTTTGCCGTGGTAAAGTAAGTGAAGGACTAGATTTTGCTGATCATGCTGGAAGAGCTGTAGTCATTACTGGTCTACCATTTGCCACAAGGAATGATCCTAAGGTTCGACTGAAACGTGAATACTTGGATCAGCAGGCATATTCCCAAAGGCAAGCATGTAAGGTTCTAACTGGAGAAGACTGGTACAATCAACAAGCATTACGAGCTGTGAATCAGGCTGTTGGGCGCGTAATCCGTCATCGTCATGATTATGGAGCAATTATTTTTTGTGATGAAAGGTTTGCACATTCAAACCGTCAATCTCAAATATCGCTCTGGATACAACCTCATATCAAGTGTTACTCTAACTTTGGGGACGTAGTTTTTTCGTTGACCCGATTCTTTCGGGATGGAGATCGGGGTCCTACTAAGCTGCAACAAACTGAAAAATGGGATGCAATTTCTACTTCAAAATCAGTACTCCATGAGCAGCCCGATCATAAACCATCTTCTCTAGGTTCATCAACTTCAGATCATGGAACCCCAGGGAAAATATATGAGTTGAAAACTGAGGAACCACCAGAAATATTTTACTTGGACCAGTTTCTACCTAAGACAACTGCAGTTGGTCAAGACCCCGCTGTAAAATCGCCTTCTTTCCTACTTAAAGCTAGAAGGGACAGAATATCAAGCCTGTTAGGCGAAGTCCTTCCTGCCAACCGCTCATCTCTTACTTGCTGTAAGGATCAGTCTGTGTCATTAAAGTATTCAAATGATCTATTTCAAAGTGAGAAAAAGATGCGTATGTGTGATAGGGGAACTATGCAATGTCAAGAAAGTAAGGTTCTTGGGTTTCCTGGGAAAAATGAGAAACGAAGTGAACAACAACTGATAGCACCTTGTTCCACAAAGAAGTATAAATTAGAGCATGGTGCAGAACAACATGACAGTTCTAAGCATTCATCTCTTGCAAAAGATGCTCACTCATATGGTCTTTACTCCACTAATAATTCAATGAGAAGTGAGAGTTCACATAGATCTGACAGTGGAAGTGCACAAAATGCTCAAGTTGGTTCAGCTCTGCTGTGCAAAGATACGAGGATCATACAACAGGGAGATGCTGAGTTTCTGAGCAAGAAAAAAACGAGTGCAATATCCAGCCCTGTGCCTTCTGGTGATGGGGAAACCAGAGGATCTGAGTTTCTAGTTCAGGTTGAGGAAAAACTTAGTGCTTTGGAATATAAAGAATTTGTAAATTTGATGAAGGCACTTAAGTCCAAAGCTATGAAGATAAATGAGGTTTTGCAATCCATTGCGGGCTTGTTTTCCGGAAGAGAGAGGCTTCCTCTTCTTAAAAGATTTAAGGACTTTATCCCTGCAAAGTATCACTCTATGTATGAGAACTATTTTCAAACAAATGACGGATCTCTTGATATCCAAGGAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000018 GO:0000723 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009555 GO:0009892 GO:0009987 GO:0010569 GO:0010605 GO:0016043 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031324 GO:0032200 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0036297 GO:0042592 GO:0043007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048229 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070716 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0099402 GO:1901360 GO:1901576 GO:2000779 GO:2001020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD_2 Helicase_C_2

Protein Analysis

1068

Amino Acids

119.76

Weight (kDa)

8.71

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD_2	PF06733	99 - 262	1e-51	DEAD_2
ARCH_RTEL1	PF23109	268 - 425	4.9e-43	RTEL1 helicase, ARCH domain
Helicase_C_2	PF13307	510 - 701	3.2e-48	Helicase C-terminal domain
HHD_RTEL1	PF23116	891 - 1057	5.6e-11	RTEL1, HHD domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950 AT1G79950
fragaria_vesca	FvH4_6g31180 FvH4_6g31180 FvH4_6g31180 FvH4_6g31180
malus_domestica	MD17G1195000.v1.1
prunus_persica	Prupe.3G071000_v2.0.a1 Prupe.3G071000_v2.0.a1 Prupe.3G071000_v2.0.a1
pyrus_communis	pycom17g20090
rosa_chinensis	RchiOBHm_Chr2g0137871
rosa_laevigata	RLG00000019686
rosa_multiflora	Rmu_sc0002170.1_g000014
rosa_roxburghii	Rroxscaffold_2G00106700
rosa_rugosa	Rorug02G0347600
rosa_samantha	Rh2AG393900 Rh2BG401300 Rh2CG380600 Rh2DG416100
rosa_wichuraiana	Rw2G032240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	660
AccBSI	CCGCTC	1 cut(s)	2478
AccI	GTMKAC	1 cut(s)	1849
AccII	CGCG	2 cut(s)	426, 2004
AccIII	TCCGGA	1 cut(s)	3083
AciI	CCGC	7 cut(s)	304, 426, 1004, 1390, 2394, 2476, 3071
AclWI	GGATC	9 cut(s)	313, 388, 668, 1865, 1911, 2508, 2864, 2956, 3187
AcsI	RAATTY	3 cut(s)	1292, 3002, 3010
AcuI	CTGAAG	4 cut(s)	834, 957, 1760, 2268
AfeI	AGCGCT	2 cut(s)	352, 906
AfiI	CCNNNNNNNGG	3 cut(s)	46, 542, 1093
AflII	CTTAAG	1 cut(s)	3026
AhdI	GACNNNNNGTC	1 cut(s)	150
AjnI	CCWGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
Alw21I	GWGCWC	2 cut(s)	627, 2812
Alw44I	GTGCAC	1 cut(s)	2808
AlwI	GGATC	9 cut(s)	313, 388, 668, 1865, 1911, 2508, 2864, 2956, 3187
AlwNI	CAGNNNCTG	3 cut(s)	767, 1369, 1757
Ama87I	CYCGRG	1 cut(s)	384
Aor13HI	TCCGGA	1 cut(s)	3083
Aor51HI	AGCGCT	2 cut(s)	352, 906
AoxI	GGCC	6 cut(s)	171, 190, 359, 596, 980, 1450
ApaI	GGGCCC	1 cut(s)	194
ApaLI	GTGCAC	1 cut(s)	2808
ApeKI	GCWGC	3 cut(s)	767, 2188, 2245
ApoI	RAATTY	3 cut(s)	1292, 3002, 3010
Asp700I	GAANNNNTTC	1 cut(s)	2463
AspLEI	GCGC	5 cut(s)	164, 353, 471, 907, 2004
AsuHPI	GGTGA	1 cut(s)	2942
AvaI	CYCGRG	1 cut(s)	384
AvaII	GGWCC	6 cut(s)	206, 251, 1513, 1594, 2177, 2353
AxyI	CCTNAGG	1 cut(s)	1875
BaeGI	GKGCMC	2 cut(s)	194, 2812
BanII	GRGCYC	3 cut(s)	194, 627, 1316
BarI	GAAGNNNNNNTAC	2 cut(s)	402, 434
BauI	CACGAG	1 cut(s)	507
BbsI	GAAGAC	4 cut(s)	146, 149, 861, 1959
Bbv12I	GWGCWC	2 cut(s)	627, 2812
BbvCI	CCTCAGC	1 cut(s)	1750
BbvI	GCAGC	3 cut(s)	754, 2175, 2257
BccI	CCATC	4 cut(s)	1217, 2159, 2269, 2927
BceAI	ACGGC	2 cut(s)	583, 1767
BcgI	CGANNNNNNTGC	4 cut(s)	13, 47, 1092, 1126
BciT130I	CCWGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
BciVI	GTATCC	3 cut(s)	472, 959, 2088
BclI	TGATCA	2 cut(s)	228, 1816
BfaI	CTAG	8 cut(s)	1245, 1376, 1565, 1689, 1805, 2270, 2427, 2960
BfmI	CTRYAG	4 cut(s)	460, 695, 1833, 2376
BfoI	RGCGCY	2 cut(s)	354, 908
BfrI	CTTAAG	1 cut(s)	3026
BfuI	GTATCC	3 cut(s)	472, 959, 2088
BglI	GCCNNNNNGGC	3 cut(s)	170, 790, 2454
BglII	AGATCT	1 cut(s)	2792
BisI	GCNGC	4 cut(s)	768, 1391, 2189, 2246
BlsI	GCNGC	4 cut(s)	769, 1392, 2190, 2247
BmcAI	AGTACT	1 cut(s)	2235
Bme1390I	CCNGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
Bme18I	GGWCC	6 cut(s)	206, 251, 1513, 1594, 2177, 2353
BmeRI	GACNNNNNGTC	1 cut(s)	150
BmeT110I	CYCGRG	1 cut(s)	384
BmiI	GGNNCC	7 cut(s)	192, 253, 597, 1686, 2178, 2296, 2331
BmrFI	CCNGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
BmrI	ACTGGG	1 cut(s)	147
BmsI	GCATC	8 cut(s)	33, 229, 251, 754, 2200, 2544, 2722, 2863
BmuI	ACTGGG	1 cut(s)	147
BpiI	GAAGAC	4 cut(s)	146, 149, 861, 1959
BplI	GAGNNNNNCTC	4 cut(s)	520, 552, 3085, 3117
BpmI	CTGGAG	4 cut(s)	140, 196, 1455, 1968
Bpu10I	CCTNAGC	2 cut(s)	1035, 1750
BpuEI	CTTGAG	3 cut(s)	941, 1073, 2805
Bsa29I	ATCGAT	1 cut(s)	27
BsaBI	GATNNNNATC	1 cut(s)	2169
BsaJI	CCNNGG	9 cut(s)	954, 977, 1347, 1516, 1783, 2299, 2300, 2612, 3193
BsaWI	WCCGGW	1 cut(s)	3083
BsaXI	ACNNNNNCTCC	4 cut(s)	169, 199, 1327, 1357
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 542, 1093
Bse118I	RCCGGY	1 cut(s)	1113
Bse1I	ACTGG	9 cut(s)	136, 142, 208, 586, 1212, 1849, 1951, 1961, 2356
Bse21I	CCTNAGG	1 cut(s)	1875
Bse3DI	GCAATG	5 cut(s)	1041, 1290, 1602, 2588, 3066
Bse8I	GATNNNNATC	1 cut(s)	2169
BseAI	TCCGGA	1 cut(s)	3083
BseBI	CCWGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
BseCI	ATCGAT	1 cut(s)	27
BseDI	CCNNGG	9 cut(s)	954, 977, 1347, 1516, 1783, 2299, 2300, 2612, 3193
BseGI	GGATG	3 cut(s)	1270, 2170, 2215
BseJI	GATNNNNATC	1 cut(s)	2169
BseLI	CCNNNNNNNGG	3 cut(s)	46, 542, 1093
BseMI	GCAATG	5 cut(s)	1041, 1290, 1602, 2588, 3066
BseMII	CTCAG	6 cut(s)	913, 1764, 2316, 2868, 2876, 2943
BseNI	ACTGG	9 cut(s)	136, 142, 208, 586, 1212, 1849, 1951, 1961, 2356
BseRI	GAGGAG	1 cut(s)	1465
BseSI	GKGCMC	2 cut(s)	194, 2812
BseXI	GCAGC	3 cut(s)	754, 2175, 2257
BsgI	GTGCAG	3 cut(s)	780, 1782, 2709
Bsh1236I	CGCG	2 cut(s)	426, 2004
BshFI	GGCC	6 cut(s)	173, 192, 361, 598, 982, 1452
BshVI	ATCGAT	1 cut(s)	27
BsiHKAI	GWGCWC	2 cut(s)	627, 2812
BsiHKCI	CYCGRG	1 cut(s)	384
BsiSI	CCGG	3 cut(s)	322, 1114, 3084
BsiWI	CGTACG	1 cut(s)	51
BslFI	GGGAC	6 cut(s)	111, 237, 286, 1016, 2144, 2447
BslI	CCNNNNNNNGG	3 cut(s)	46, 542, 1093
BsmFI	GGGAC	6 cut(s)	111, 237, 286, 1016, 2144, 2447
BsmI	GAATGC	1 cut(s)	2713
BsnI	GGCC	6 cut(s)	173, 192, 361, 598, 982, 1452
BsoBI	CYCGRG	1 cut(s)	384
Bsp120I	GGGCCC	1 cut(s)	190
Bsp1286I	GDGCHC	4 cut(s)	194, 627, 1316, 2812
Bsp13I	TCCGGA	1 cut(s)	3083
Bsp1407I	TGTACA	1 cut(s)	1195
BspACI	CCGC	7 cut(s)	304, 426, 1004, 1390, 2394, 2476, 3071
BspANI	GGCC	6 cut(s)	173, 192, 361, 598, 982, 1452
BspCNI	CTCAG	6 cut(s)	912, 1763, 2317, 2869, 2877, 2944
BspDI	ATCGAT	1 cut(s)	27
BspEI	TCCGGA	1 cut(s)	3083
BspFNI	CGCG	2 cut(s)	426, 2004
BspHI	TCATGA	1 cut(s)	2020
BspLI	GGNNCC	7 cut(s)	192, 253, 597, 1686, 2178, 2296, 2331
BspMAI	CTGCAG	2 cut(s)	464, 2380
BspPI	GGATC	9 cut(s)	313, 388, 668, 1865, 1911, 2508, 2864, 2956, 3187
BspQI	GCTCTTC	1 cut(s)	1822
BspTI	CTTAAG	1 cut(s)	3026
BsrBI	CCGCTC	1 cut(s)	2478
BsrDI	GCAATG	5 cut(s)	1041, 1290, 1602, 2588, 3066
BsrFI	RCCGGY	1 cut(s)	1113
BsrGI	TGTACA	1 cut(s)	1195
BsrI	ACTGG	9 cut(s)	136, 142, 208, 586, 1212, 1849, 1951, 1961, 2356
BssAI	RCCGGY	1 cut(s)	1113
BssECI	CCNNGG	9 cut(s)	954, 977, 1347, 1516, 1783, 2299, 2300, 2612, 3193
BssSI	CACGAG	1 cut(s)	507
BssT1I	CCWWGG	4 cut(s)	954, 1347, 1516, 3193
Bst2BI	CACGAG	1 cut(s)	507
Bst2UI	CCWGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
Bst4CI	ACNGT	7 cut(s)	264, 716, 1030, 1066, 2072, 2705, 2801
Bst6I	CTCTTC	5 cut(s)	176, 521, 1822, 3082, 3108
BstAFI	CTTAAG	1 cut(s)	3026
BstAPI	GCANNNNNTGC	4 cut(s)	767, 1608, 2732, 2816
BstAUI	TGTACA	1 cut(s)	1195
BstC8I	GCNNGC	9 cut(s)	62, 448, 1111, 1115, 1450, 1609, 1911, 1929, 3073
BstDSI	CCRYGG	1 cut(s)	1783
BstF5I	GGATG	3 cut(s)	1270, 2170, 2215
BstFNI	CGCG	2 cut(s)	426, 2004
BstH2I	RGCGCY	2 cut(s)	354, 908
BstHHI	GCGC	5 cut(s)	164, 353, 471, 907, 2004
BstNI	CCWGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
BstNSI	RCATGY	2 cut(s)	1138, 1935
BstSCI	CCNGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
BstSFI	CTRYAG	4 cut(s)	460, 695, 1833, 2376
BstSLI	GKGCMC	2 cut(s)	194, 2812
BstUI	CGCG	2 cut(s)	426, 2004
BstV1I	GCAGC	3 cut(s)	754, 2175, 2257
BstV2I	GAAGAC	4 cut(s)	146, 149, 861, 1959
BstX2I	RGATCY	5 cut(s)	318, 380, 2792, 2948, 3179
BstXI	CCANNNNNNTGG	1 cut(s)	961
BstYI	RGATCY	5 cut(s)	318, 380, 2792, 2948, 3179
Bsu15I	ATCGAT	1 cut(s)	27
Bsu36I	CCTNAGG	1 cut(s)	1875
BsuI	GTATCC	3 cut(s)	472, 959, 2088
BsuRI	GGCC	6 cut(s)	173, 192, 361, 598, 982, 1452
BsuTUI	ATCGAT	1 cut(s)	27
BtgI	CCRYGG	1 cut(s)	1783
BtsCI	GGATG	3 cut(s)	1270, 2170, 2215
BtsI	GCAGTG	1 cut(s)	113
BtsIMutI	CAGTG	4 cut(s)	113, 129, 1219, 2806
Cac8I	GCNNGC	9 cut(s)	62, 448, 1111, 1115, 1450, 1609, 1911, 1929, 3073
CaiI	CAGNNNCTG	3 cut(s)	767, 1369, 1757
CciI	TCATGA	1 cut(s)	2020
CfoI	GCGC	5 cut(s)	164, 353, 471, 907, 2004
Cfr10I	RCCGGY	1 cut(s)	1113
ClaI	ATCGAT	1 cut(s)	27
CseI	GACGC	2 cut(s)	476, 1067
DraI	TTTAAA	2 cut(s)	1011, 1230
DriI	GACNNNNNGTC	1 cut(s)	150
Eam1104I	CTCTTC	5 cut(s)	176, 521, 1822, 3082, 3108
Eam1105I	GACNNNNNGTC	1 cut(s)	150
EarI	CTCTTC	5 cut(s)	176, 521, 1822, 3082, 3108
EciI	GGCGGA	1 cut(s)	1019
Ecl136II	GAGCTC	1 cut(s)	625
Eco130I	CCWWGG	4 cut(s)	954, 1347, 1516, 3193
Eco24I	GRGCYC	3 cut(s)	194, 627, 1316
Eco32I	GATATC	2 cut(s)	562, 3190
Eco47I	GGWCC	6 cut(s)	206, 251, 1513, 1594, 2177, 2353
Eco47III	AGCGCT	2 cut(s)	352, 906
Eco53kI	GAGCTC	1 cut(s)	625
Eco57I	CTGAAG	4 cut(s)	834, 957, 1760, 2268
Eco81I	CCTNAGG	1 cut(s)	1875
Eco88I	CYCGRG	1 cut(s)	384
EcoICRI	GAGCTC	1 cut(s)	625
EcoO109I	RGGNCCY	3 cut(s)	191, 1513, 2177
EcoRII	CCWGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
EcoRV	GATATC	2 cut(s)	562, 3190
EcoT14I	CCWWGG	4 cut(s)	954, 1347, 1516, 3193
EcoT22I	ATGCAT	3 cut(s)	244, 401, 1604
EcoT38I	GRGCYC	3 cut(s)	194, 627, 1316
ErhI	CCWWGG	4 cut(s)	954, 1347, 1516, 3193
FalI	AAGNNNNNCTT	2 cut(s)	337, 369
FaqI	GGGAC	6 cut(s)	111, 237, 286, 1016, 2144, 2447
FauI	CCCGC	3 cut(s)	311, 2401, 3064
FauNDI	CATATG	2 cut(s)	755, 2743
FbaI	TGATCA	2 cut(s)	228, 1816
FblI	GTMKAC	1 cut(s)	1849
Fnu4HI	GCNGC	4 cut(s)	768, 1391, 2189, 2246
FokI	GGATG	3 cut(s)	1257, 2177, 2222
FriOI	GRGCYC	3 cut(s)	194, 627, 1316
Fsp4HI	GCNGC	4 cut(s)	768, 1391, 2189, 2246
FspBI	CTAG	8 cut(s)	1245, 1376, 1565, 1689, 1805, 2270, 2427, 2960
GlaI	GCGC	5 cut(s)	163, 352, 470, 906, 2003
GluI	GCNGC	4 cut(s)	768, 1391, 2189, 2246
GsuI	CTGGAG	4 cut(s)	140, 196, 1455, 1968
HaeII	RGCGCY	2 cut(s)	354, 908
HaeIII	GGCC	6 cut(s)	173, 192, 361, 598, 982, 1452
HapII	CCGG	3 cut(s)	322, 1114, 3084
HgaI	GACGC	2 cut(s)	476, 1067
HhaI	GCGC	5 cut(s)	164, 353, 471, 907, 2004
Hin6I	GCGC	5 cut(s)	162, 351, 469, 905, 2002
HinP1I	GCGC	5 cut(s)	162, 351, 469, 905, 2002
HincII	GTYRAC	1 cut(s)	2148
HindII	GTYRAC	1 cut(s)	2148
HindIII	AAGCTT	1 cut(s)	917
HpaII	CCGG	3 cut(s)	322, 1114, 3084
HphI	GGTGA	1 cut(s)	2942
Hpy166II	GTNNAC	7 cut(s)	70, 419, 1850, 2148, 2636, 2786, 2810
Hpy8I	GTNNAC	7 cut(s)	70, 419, 1850, 2148, 2636, 2786, 2810
Hpy99I	CGWCG	3 cut(s)	10, 492, 1582
HpyCH4III	ACNGT	7 cut(s)	264, 716, 1030, 1066, 2072, 2705, 2801
HpyCH4IV	ACGT	4 cut(s)	8, 299, 1891, 2133
HpySE526I	ACGT	4 cut(s)	8, 299, 1891, 2133
HspAI	GCGC	5 cut(s)	162, 351, 469, 905, 2002
Kpn2I	TCCGGA	1 cut(s)	3083
KroI	GCCGGC	1 cut(s)	1113
KroNI	GCCGGC	1 cut(s)	1115
Ksp22I	TGATCA	2 cut(s)	228, 1816
LguI	GCTCTTC	1 cut(s)	1822
LmnI	GCTCC	3 cut(s)	622, 630, 2030
Lsp1109I	GCAGC	3 cut(s)	754, 2175, 2257
LweI	GCATC	8 cut(s)	33, 229, 251, 754, 2200, 2544, 2722, 2863
MaeI	CTAG	8 cut(s)	1245, 1376, 1565, 1689, 1805, 2270, 2427, 2960
MaeII	ACGT	4 cut(s)	8, 299, 1891, 2133
MaeIII	GTNAC	4 cut(s)	258, 268, 1130, 2114
MbiI	CCGCTC	1 cut(s)	2478
MfeI	CAATTG	1 cut(s)	1716
MflI	RGATCY	5 cut(s)	318, 380, 2792, 2948, 3179
MhlI	GDGCHC	4 cut(s)	194, 627, 1316, 2812
MlyI	GAGTC	2 cut(s)	133, 851
Mph1103I	ATGCAT	3 cut(s)	244, 401, 1604
MroI	TCCGGA	1 cut(s)	3083
MroNI	GCCGGC	1 cut(s)	1113
MroXI	GAANNNNTTC	1 cut(s)	2463
MseI	TTAA	8 cut(s)	1010, 1140, 1229, 2421, 2516, 3027, 3108, 3117
MslI	CAYNNNNRTG	3 cut(s)	2019, 2025, 2742
MspA1I	CMGCKG	2 cut(s)	1448, 2396
MspCI	CTTAAG	1 cut(s)	3026
MspI	CCGG	3 cut(s)	322, 1114, 3084
MspR9I	CCNGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
MunI	CAATTG	1 cut(s)	1716
Mva1269I	GAATGC	1 cut(s)	2713
MvaI	CCWGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
MvnI	CGCG	2 cut(s)	426, 2004
NaeI	GCCGGC	1 cut(s)	1115
NdeI	CATATG	2 cut(s)	755, 2743
NgoMIV	GCCGGC	1 cut(s)	1113
NlaIV	GGNNCC	7 cut(s)	192, 253, 597, 1686, 2178, 2296, 2331
NmuCI	GTSAC	3 cut(s)	258, 268, 1130
NsiI	ATGCAT	3 cut(s)	244, 401, 1604
NspI	RCATGY	2 cut(s)	1138, 1935
PaeR7I	CTCGAG	1 cut(s)	384
PagI	TCATGA	1 cut(s)	2020
PasI	CCCWGGG	1 cut(s)	2300
PciSI	GCTCTTC	1 cut(s)	1822
PcsI	WCGNNNNNNNCGW	1 cut(s)	1888
PctI	GAATGC	1 cut(s)	2713
PdiI	GCCGGC	1 cut(s)	1115
PdmI	GAANNNNTTC	1 cut(s)	2463
Pfl23II	CGTACG	1 cut(s)	51
PfoI	TCCNGGA	1 cut(s)	1273
PkrI	GCNGC	4 cut(s)	769, 1392, 2190, 2247
PleI	GAGTC	2 cut(s)	132, 851
PpsI	GAGTC	2 cut(s)	132, 851
PpuMI	RGGWCCY	2 cut(s)	1513, 2177
PsiI	TTATAA	1 cut(s)	660
Psp124BI	GAGCTC	1 cut(s)	627
Psp5II	RGGWCCY	2 cut(s)	1513, 2177
Psp6I	CCWGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
PspGI	CCWGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
PspLI	CGTACG	1 cut(s)	51
PspN4I	GGNNCC	7 cut(s)	192, 253, 597, 1686, 2178, 2296, 2331
PspOMI	GGGCCC	1 cut(s)	190
PspPPI	RGGWCCY	2 cut(s)	1513, 2177
PstI	CTGCAG	2 cut(s)	464, 2380
PstNI	CAGNNNCTG	3 cut(s)	767, 1369, 1757
PsuI	RGATCY	5 cut(s)	318, 380, 2792, 2948, 3179
PvuII	CAGCTG	1 cut(s)	1448
RseI	CAYNNNNRTG	3 cut(s)	2019, 2025, 2742
SacI	GAGCTC	1 cut(s)	627
SapI	GCTCTTC	1 cut(s)	1822
SaqAI	TTAA	8 cut(s)	1010, 1140, 1229, 2421, 2516, 3027, 3108, 3117
SatI	GCNGC	4 cut(s)	768, 1391, 2189, 2246
ScaI	AGTACT	1 cut(s)	2235
SchI	GAGTC	2 cut(s)	133, 851
ScrFI	CCNGG	9 cut(s)	175, 746, 978, 1001, 1088, 1275, 1300, 2301, 2613
SduI	GDGCHC	4 cut(s)	194, 627, 1316, 2812
SfaNI	GCATC	8 cut(s)	33, 229, 251, 754, 2200, 2544, 2722, 2863
SfcI	CTRYAG	4 cut(s)	460, 695, 1833, 2376
Sfr274I	CTCGAG	1 cut(s)	384
SinI	GGWCC	6 cut(s)	206, 251, 1513, 1594, 2177, 2353
SlaI	CTCGAG	1 cut(s)	384
SmiMI	CAYNNNNRTG	3 cut(s)	2019, 2025, 2742
SmlI	CTYRAG	5 cut(s)	384, 920, 1052, 2820, 3026
SmoI	CTYRAG	5 cut(s)	384, 920, 1052, 2820, 3026
SsiI	CCGC	7 cut(s)	304, 426, 1004, 1390, 2394, 2476, 3071
SspI	AATATT	1 cut(s)	2343
SspMI	CTAG	8 cut(s)	1245, 1376, 1565, 1689, 1805, 2270, 2427, 2960
SstI	GAGCTC	1 cut(s)	627
StyD4I	CCNGG	9 cut(s)	173, 744, 976, 999, 1086, 1273, 1298, 2299, 2611
StyI	CCWWGG	4 cut(s)	954, 1347, 1516, 3193
TaaI	ACNGT	7 cut(s)	264, 716, 1030, 1066, 2072, 2705, 2801
TaiI	ACGT	4 cut(s)	11, 302, 1894, 2136
TaqI	TCGA	9 cut(s)	27, 33, 385, 564, 617, 948, 1102, 1543, 1882
TatI	WGTACW	4 cut(s)	1066, 1195, 1503, 2233
TauI	GCSGC	1 cut(s)	1393
Tru1I	TTAA	8 cut(s)	1010, 1140, 1229, 2421, 2516, 3027, 3108, 3117
Tru9I	TTAA	8 cut(s)	1010, 1140, 1229, 2421, 2516, 3027, 3108, 3117
TscAI	CASTG	4 cut(s)	120, 136, 1219, 2806
TseFI	GTSAC	3 cut(s)	258, 268, 1130
TseI	GCWGC	3 cut(s)	767, 2188, 2245
Tsp45I	GTSAC	3 cut(s)	258, 268, 1130
TspDTI	ATGAA	9 cut(s)	564, 1008, 1181, 1259, 2064, 2265, 2706, 3032, 3056
TspGWI	ACGGA	4 cut(s)	1514, 1566, 2000, 3192
TspRI	CASTG	4 cut(s)	120, 136, 1219, 2806
Vha464I	CTTAAG	1 cut(s)	3026
VneI	GTGCAC	1 cut(s)	2808
VpaK11BI	GGWCC	6 cut(s)	206, 251, 1513, 1594, 2177, 2353
XapI	RAATTY	3 cut(s)	1292, 3002, 3010
XceI	RCATGY	2 cut(s)	1138, 1935
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1306
XhoI	CTCGAG	1 cut(s)	384
XmiI	GTMKAC	1 cut(s)	1849
XmnI	GAANNNNTTC	1 cut(s)	2463
XspI	CTAG	8 cut(s)	1245, 1376, 1565, 1689, 1805, 2270, 2427, 2960
ZrmI	AGTACT	1 cut(s)	2235
Zsp2I	ATGCAT	3 cut(s)	244, 401, 1604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.