FvH4_6g31740

Photosystem I reaction center subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013380
Physical Location & Seq
Reverse (-)
24848161 .. 24849587
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31740.t1

Sequence Viewer

Length: 435 bp
ATGGCATCTTTGGCAACCTTGGCAGCTGTTCAACCGGCCACCATCAACGGCCTTGGAGGAAGCTCCCTCTCTAGGACTAAGCTCGTCATCAAGCCCACTCGCCAGACCCTCAGACCCAGAAATGTCAGGAGTGGTGCTGTGGTGGCCAAGTATGGTGACAAAAGTGTCTACTTTGACCTTGAGGACCTGGGCAACACCACTGGAAAATGGGACTTGTACGGCTCAGATGCACCCTCACCTTACAACCCTCTTCAGAGCAAGTTCTTTGAGACATTTGCTGCTCCATTCACCAAGAGAGGTCTGCTGCTCAAGTTCTTGATCTTGGGAGGGGCTTCCACCATTGCTTACCTTAGTGCCACTGCCCCAGATGATCTTCTACCCATCAAGAAGGGCCCACAACTTCCCCCAAAGTTGGGACCACGTGGCAAGATCTGA

Protein Analysis

145

Amino Acids

15.36

Weight (kDa)

10.04

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaH PF03244 7 - 144 8.8e-70 Photosystem I reaction centre subunit VI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52230 AT3G16140
fragaria_vesca FvH4_6g31740
malus_domestica MD09G1209500.v1.1
prunus_persica Prupe.3G067400_v2.0.a1
pyrus_communis pycom09g12570 pycom17g19830
rosa_chinensis RchiOBHm_Chr2g0138931
rosa_laevigata RLG00000019769
rosa_multiflora Rmu_sc0001376.1_g000002
rosa_roxburghii Rroxscaffold_2G00105860
rosa_rugosa Rorug02G0353200 Rorug02G0353300
rosa_samantha Rh2AG403300 Rh2BG413800 Rh2CG389400 Rh2DG423600
rosa_wichuraiana Rw2G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 168
AcoI YGGCCR 2 cut(s) 36, 144
AcuI CTGAAG 1 cut(s) 236
AcvI CACGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 3 cut(s) 72, 412, 413
AgsI TTSAA 1 cut(s) 32
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 3 cut(s) 26, 63, 82
AluI AGCT 3 cut(s) 26, 63, 82
Alw26I GTCTC 1 cut(s) 263
AoxI GGCC 4 cut(s) 36, 49, 144, 391
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 3 cut(s) 23, 278, 304
AspS9I GGNCC 4 cut(s) 184, 391, 392, 416
AsuHPI GGTGA 3 cut(s) 167, 228, 280
AvaII GGWCC 2 cut(s) 184, 416
BaeGI GKGCMC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 395
BbrPI CACGTG 1 cut(s) 422
BbvI GCAGC 3 cut(s) 35, 265, 291
BccI CCATC 2 cut(s) 50, 389
BceAI ACGGC 2 cut(s) 64, 235
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 72
BglII AGATCT 1 cut(s) 429
BisI GCNGC 3 cut(s) 24, 279, 305
BlsI GCNGC 3 cut(s) 25, 280, 306
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 2 cut(s) 184, 416
BmgT120I GGNCC 4 cut(s) 184, 391, 392, 416
BmiI GGNNCC 2 cut(s) 393, 417
BmrFI CCNGG 1 cut(s) 188
BmsI GCATC 2 cut(s) 14, 217
BpuEI CTTGAG 2 cut(s) 200, 293
BsaAI YACGTR 1 cut(s) 422
BsaJI CCNNGG 3 cut(s) 18, 52, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 412, 413
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 18, 52, 187
BseLI CCNNNNNNNGG 3 cut(s) 72, 412, 413
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 2 cut(s) 124, 237
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 35, 265, 291
BshFI GGCC 4 cut(s) 38, 51, 146, 393
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 224, 429
BslI CCNNNNNNNGG 3 cut(s) 72, 412, 413
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 224, 429
BsnI GGCC 4 cut(s) 38, 51, 146, 393
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 3 cut(s) 318, 370, 429
BspANI GGCC 4 cut(s) 38, 51, 146, 393
BspCNI CTCAG 2 cut(s) 123, 236
BspLI GGNNCC 2 cut(s) 393, 417
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 18, 52, 187
BssMI GATC 3 cut(s) 318, 370, 429
BssT1I CCWWGG 2 cut(s) 18, 52
Bst2UI CCWGG 1 cut(s) 188
Bst6I CTCTTC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 422
BstDEI CTNAG 4 cut(s) 78, 110, 223, 350
BstKTI GATC 3 cut(s) 321, 373, 432
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 318, 370, 429
BstMWI GCNNNNNNNGC 3 cut(s) 11, 20, 143
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 35, 265, 291
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 4 cut(s) 38, 51, 146, 393
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 198, 357
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 4 cut(s) 184, 391, 392, 416
Csp6I GTAC 1 cut(s) 217
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 4 cut(s) 78, 110, 223, 350
DpnI GATC 3 cut(s) 320, 372, 431
DpnII GATC 3 cut(s) 318, 370, 429
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 36, 144
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 2 cut(s) 18, 52
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 184, 416
Eco57I CTGAAG 1 cut(s) 236
Eco72I CACGTG 1 cut(s) 422
EcoO109I RGGNCCY 2 cut(s) 184, 391
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 18, 52
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 2 cut(s) 18, 52
FaiI YATR 1 cut(s) 153
FaqI GGGAC 2 cut(s) 224, 429
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 24, 279, 305
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 24, 279, 305
FspBI CTAG 1 cut(s) 72
GluI GCNGC 3 cut(s) 24, 279, 305
HaeIII GGCC 4 cut(s) 38, 51, 146, 393
HapII CCGG 1 cut(s) 35
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 167, 228, 280
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 4 cut(s) 113, 226, 255, 434
Hpy188III TCNNGA 3 cut(s) 127, 316, 385
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 20, 143
HpyF3I CTNAG 4 cut(s) 78, 110, 223, 350
HpySE526I ACGT 1 cut(s) 421
Kzo9I GATC 3 cut(s) 318, 370, 429
LmnI GCTCC 2 cut(s) 68, 286
LpnPI CCDG 8 cut(s) 48, 112, 116, 130, 173, 186, 200, 378
Lsp1109I GCAGC 3 cut(s) 35, 265, 291
LweI GCATC 2 cut(s) 14, 217
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 1 cut(s) 155
MalI GATC 3 cut(s) 320, 372, 431
MboI GATC 3 cut(s) 318, 370, 429
MboII GAAGA 2 cut(s) 242, 365
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 146
MnlI CCTC 8 cut(s) 50, 77, 119, 175, 244, 258, 290, 320
Mox20I TGGCCA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 11, 20, 143
NdeII GATC 3 cut(s) 318, 370, 429
NlaIV GGNNCC 2 cut(s) 393, 417
NmuCI GTSAC 1 cut(s) 155
PkrI GCNGC 3 cut(s) 25, 280, 306
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 422
PpuMI RGGWCCY 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 186
PspCI CACGTG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 2 cut(s) 393, 417
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 4 cut(s) 184, 391, 392, 416
PspPPI RGGWCCY 1 cut(s) 184
PsuI RGATCY 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SatI GCNGC 3 cut(s) 24, 279, 305
Sau3AI GATC 3 cut(s) 318, 370, 429
Sau96I GGNCC 4 cut(s) 184, 391, 392, 416
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 2 cut(s) 14, 217
SinI GGWCC 2 cut(s) 184, 416
SmlI CTYRAG 2 cut(s) 179, 308
SmoI CTYRAG 2 cut(s) 179, 308
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 2 cut(s) 18, 52
TaiI ACGT 1 cut(s) 424
TscAI CASTG 2 cut(s) 205, 364
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 3 cut(s) 23, 278, 304
Tsp45I GTSAC 1 cut(s) 155
TspRI CASTG 2 cut(s) 205, 364
VpaK11BI GGWCC 2 cut(s) 184, 416
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.