pycom09g12570

Photosystem I reaction center subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013380
Physical Location & Seq
Forward (+)
11159164 .. 11160449
1286 bp
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UTR
Exon/CDS
Intron
pycom09g12570.1

Sequence Viewer

Length: 435 bp
ATGGCATCTTTGGCAACCCTAGCAGCTGTTCAACCTGCCGCCATTAACGGCCTCGGCGGAAGCTCCCTCACCGGAACAAAGCTTGTTGTGAAGCCCACTCGCCAGAGCATCAGAACCAAACACATCAGGAGTGGTGCTGTGGTGGCCAAGTACGGAGACAAGAGCGTCTACTTTGACCTTGAGGATCTGGGCAACACCACCGGCAAGTGGGACTTGTATGGTTCGGATGCGCCTTCACCCTACAACCCTCTTCAGAGCAAGTTCTTTGAGACGTTTGCTGCTCCATTCACCAAGAGAGGCTTACTCCTCAAGTTCTTGATATTGGGAGGAGCCTCCACCATTGCCTACCTTAGTGCCACTGCCTCCGATGACATTCTACCAATCAAGAAGGGCCCACAACTTCCACCCAAGTTGGGGCCACGTGGCAAGATCTAA

Protein Analysis

145

Amino Acids

15.22

Weight (kDa)

9.9

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaH PF03244 7 - 144 8e-72 Photosystem I reaction centre subunit VI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52230 AT3G16140
fragaria_vesca FvH4_6g31740
malus_domestica MD09G1209500.v1.1
prunus_persica Prupe.3G067400_v2.0.a1
pyrus_communis pycom09g12570 pycom17g19830
rosa_chinensis RchiOBHm_Chr2g0138931
rosa_laevigata RLG00000019769
rosa_multiflora Rmu_sc0001376.1_g000002
rosa_roxburghii Rroxscaffold_2G00105860
rosa_rugosa Rorug02G0353200 Rorug02G0353300
rosa_samantha Rh2AG403300 Rh2BG413800 Rh2CG389400 Rh2DG423600
rosa_wichuraiana Rw2G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 168
AciI CCGC 2 cut(s) 39, 57
AclWI GGATC 1 cut(s) 192
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 236
AcvI CACGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 3 cut(s) 207, 413, 414
AgsI TTSAA 1 cut(s) 32
AluBI AGCT 3 cut(s) 26, 63, 82
AluI AGCT 3 cut(s) 26, 63, 82
Alw26I GTCTC 2 cut(s) 150, 263
AlwI GGATC 1 cut(s) 192
AoxI GGCC 4 cut(s) 49, 144, 391, 416
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 2 cut(s) 23, 278
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 391, 392, 416
AsuHPI GGTGA 3 cut(s) 61, 228, 280
BaeGI GKGCMC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 395
BbrPI CACGTG 1 cut(s) 422
BbvI GCAGC 2 cut(s) 35, 265
BceAI ACGGC 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 150, 263
BfaI CTAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 429
BisI GCNGC 3 cut(s) 24, 39, 279
BlsI GCNGC 3 cut(s) 25, 40, 280
BmgT120I GGNCC 3 cut(s) 391, 392, 416
BmiI GGNNCC 3 cut(s) 331, 393, 417
BmsI GCATC 3 cut(s) 14, 117, 217
BplI GAGNNNNNCTC 2 cut(s) 288, 320
BpuEI CTTGAG 2 cut(s) 200, 293
BsaAI YACGTR 1 cut(s) 422
BsaJI CCNNGG 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 413, 414
Bse118I RCCGGY 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 3 cut(s) 207, 413, 414
BseMI GCAATG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 296, 342
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 2 cut(s) 35, 265
BshFI GGCC 4 cut(s) 51, 146, 393, 418
BsiSI CCGG 2 cut(s) 72, 201
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 207, 413, 414
BsmAI GTCTC 2 cut(s) 150, 263
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 4 cut(s) 51, 146, 393, 418
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 184, 429
BspACI CCGC 2 cut(s) 39, 57
BspANI GGCC 4 cut(s) 51, 146, 393, 418
BspLI GGNNCC 3 cut(s) 331, 393, 417
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 2 cut(s) 184, 429
Bst6I CTCTTC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 232
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 2 cut(s) 187, 432
BstMAI GTCTC 2 cut(s) 150, 263
BstMBI GATC 2 cut(s) 184, 429
BstMWI GCNNNNNNNGC 3 cut(s) 11, 20, 143
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 35, 265
BstX2I RGATCY 2 cut(s) 184, 429
BstYI RGATCY 2 cut(s) 184, 429
BsuRI GGCC 4 cut(s) 51, 146, 393, 418
BtsCI GGATG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 391, 392, 416
CseI GACGC 1 cut(s) 154
Csp6I GTAC 1 cut(s) 151
CspCI CAANNNNNGTGG 2 cut(s) 393, 428
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 1 cut(s) 350
DpnI GATC 2 cut(s) 186, 431
DpnII GATC 2 cut(s) 184, 429
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 236
Eco72I CACGTG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 395
Esp3I CGTCTC 1 cut(s) 263
FaiI YATR 1 cut(s) 219
FalI AAGNNNNNCTT 4 cut(s) 197, 229, 284, 316
FaqI GGGAC 1 cut(s) 224
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 24, 39, 279
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 24, 39, 279
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 231
GluI GCNGC 3 cut(s) 24, 39, 279
HaeIII GGCC 4 cut(s) 51, 146, 393, 418
HapII CCGG 2 cut(s) 72, 201
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 232
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 80
HpaII CCGG 2 cut(s) 72, 201
HphI GGTGA 3 cut(s) 61, 228, 280
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 4 cut(s) 113, 226, 255, 367
Hpy188III TCNNGA 3 cut(s) 127, 316, 385
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 2 cut(s) 243, 382
HpyCH4IV ACGT 2 cut(s) 272, 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 20, 143
HpyF3I CTNAG 1 cut(s) 350
HpySE526I ACGT 2 cut(s) 272, 421
HspAI GCGC 1 cut(s) 230
Kzo9I GATC 2 cut(s) 184, 429
LmnI GCTCC 3 cut(s) 68, 286, 329
LpnPI CCDG 6 cut(s) 48, 85, 112, 116, 173, 214
Lsp1109I GCAGC 2 cut(s) 35, 265
LweI GCATC 3 cut(s) 14, 117, 217
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 272, 421
MalI GATC 2 cut(s) 186, 431
MboI GATC 2 cut(s) 184, 429
MboII GAAGA 1 cut(s) 242
MflI RGATCY 2 cut(s) 184, 429
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 146
MnlI CCTC 9 cut(s) 62, 77, 175, 258, 290, 317, 320, 343, 373
Mox20I TGGCCA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 146
MseI TTAA 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 72, 201
MwoI GCNNNNNNNGC 3 cut(s) 11, 20, 143
NdeII GATC 2 cut(s) 184, 429
NlaIV GGNNCC 3 cut(s) 331, 393, 417
NmeAIII GCCGAG 1 cut(s) 33
PkrI GCNGC 3 cut(s) 25, 40, 280
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 422
PspCI CACGTG 1 cut(s) 422
PspN4I GGNNCC 3 cut(s) 331, 393, 417
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 3 cut(s) 391, 392, 416
PsuI RGATCY 2 cut(s) 184, 429
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 3 cut(s) 24, 39, 279
Sau3AI GATC 2 cut(s) 184, 429
Sau96I GGNCC 3 cut(s) 391, 392, 416
SduI GDGCHC 1 cut(s) 395
SetI ASST 8 cut(s) 28, 37, 65, 84, 180, 275, 351, 424
SfaNI GCATC 3 cut(s) 14, 117, 217
SmlI CTYRAG 2 cut(s) 179, 308
SmoI CTYRAG 2 cut(s) 179, 308
SsiI CCGC 2 cut(s) 39, 57
SspMI CTAG 1 cut(s) 20
TaiI ACGT 2 cut(s) 275, 424
TauI GCSGC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 364
TseI GCWGC 2 cut(s) 23, 278
TspGWI ACGGA 1 cut(s) 168
TspRI CASTG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.