Rw2G032810

Photosystem I reaction center subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013380
Physical Location & Seq
Reverse (-)
53869367 .. 53870553
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032810.1

Sequence Viewer

Length: 435 bp
ATGGCATCTTTGGCAACCTTGGCAGCAGTTCAACCAGCCACCATCAACGGCCTTGGAGGAAGCTCCCTCTCCGGGACTAAGCTCGTCGTCAAGCCCACTCGCCAGACCCTCAGACCCAGAAATGTCAGGAGTGGTGCTGTGGTGGCCAAGTATGGTGACAAAAGTGTTTACTTTGACCTTGAGGATCTGGGCAACACCACTGGAAAATGGGACTTGTACGGCTCAGATGCCCCCTCACCTTACAACCCTCTTCAGAGCAAGTTCTTTGAGACATTTGCTGCTCCATTCACCAAGAGAGGGTTGCTGCTCAAGTTCTTGATCTTGGGAGGGGCTTCCACCATTGCTTACCTTAGTGCCACTGCCTCAGATGACCTTCTACCCATTAAGAAGGGCCCACAACTTCCCCCAAAGCTGGGACCACGTGGCAAGCTCTGA

Protein Analysis

144

Amino Acids

15.24

Weight (kDa)

9.94

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PsaH PF03244 7 - 144 3.9e-71 Photosystem I reaction centre subunit VI
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52230 AT3G16140
fragaria_vesca FvH4_6g31740
malus_domestica MD09G1209500.v1.1
prunus_persica Prupe.3G067400_v2.0.a1
pyrus_communis pycom09g12570 pycom17g19830
rosa_chinensis RchiOBHm_Chr2g0138931
rosa_laevigata RLG00000019769
rosa_multiflora Rmu_sc0001376.1_g000002
rosa_roxburghii Rroxscaffold_2G00105860
rosa_rugosa Rorug02G0353200 Rorug02G0353300
rosa_samantha Rh2AG403300 Rh2BG413800 Rh2CG389400 Rh2DG423600
rosa_wichuraiana Rw2G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 192
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 236
AcvI CACGTG 1 cut(s) 422
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 4 cut(s) 72, 297, 412, 413
AgsI TTSAA 1 cut(s) 32
AluBI AGCT 4 cut(s) 63, 82, 412, 430
AluI AGCT 4 cut(s) 63, 82, 412, 430
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 192
AoxI GGCC 3 cut(s) 49, 144, 391
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 3 cut(s) 23, 278, 304
AspS9I GGNCC 3 cut(s) 391, 392, 416
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 3 cut(s) 167, 228, 280
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 395
BalI TGGCCA 1 cut(s) 146
BanII GRGCYC 1 cut(s) 395
BbrPI CACGTG 1 cut(s) 422
BbvI GCAGC 3 cut(s) 35, 265, 291
BccI CCATC 1 cut(s) 50
BceAI ACGGC 2 cut(s) 64, 235
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 263
BisI GCNGC 3 cut(s) 24, 279, 305
BlsI GCNGC 3 cut(s) 25, 280, 306
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 391, 392, 416
BmiI GGNNCC 2 cut(s) 393, 417
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 2 cut(s) 14, 217
BpuEI CTTGAG 2 cut(s) 200, 293
BpuMI CCSGG 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 18, 52
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 297, 412, 413
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 18, 52
BseLI CCNNNNNNNGG 4 cut(s) 72, 297, 412, 413
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 3 cut(s) 124, 237, 378
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 35, 265, 291
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 3 cut(s) 51, 146, 393
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 3 cut(s) 88, 224, 429
BslI CCNNNNNNNGG 4 cut(s) 72, 297, 412, 413
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 3 cut(s) 88, 224, 429
BsnI GGCC 3 cut(s) 51, 146, 393
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 184, 318
BspANI GGCC 3 cut(s) 51, 146, 393
BspCNI CTCAG 3 cut(s) 123, 236, 377
BspLI GGNNCC 2 cut(s) 393, 417
BspPI GGATC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 18, 52
BssMI GATC 2 cut(s) 184, 318
BssT1I CCWWGG 2 cut(s) 18, 52
Bst6I CTCTTC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 428
BstDEI CTNAG 5 cut(s) 78, 110, 223, 350, 364
BstKTI GATC 2 cut(s) 187, 321
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 184, 318
BstMWI GCNNNNNNNGC 3 cut(s) 11, 20, 143
BstSCI CCNGG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 35, 265, 291
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 3 cut(s) 51, 146, 393
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 198, 357
Cac8I GCNNGC 1 cut(s) 428
Cfr13I GGNCC 3 cut(s) 391, 392, 416
Csp6I GTAC 1 cut(s) 217
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 5 cut(s) 78, 110, 223, 350, 364
DpnI GATC 2 cut(s) 186, 320
DpnII GATC 2 cut(s) 184, 318
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 2 cut(s) 18, 52
Eco24I GRGCYC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 236
Eco72I CACGTG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 391
EcoT14I CCWWGG 2 cut(s) 18, 52
EcoT38I GRGCYC 1 cut(s) 395
ErhI CCWWGG 2 cut(s) 18, 52
FaiI YATR 1 cut(s) 153
FaqI GGGAC 3 cut(s) 88, 224, 429
Fnu4HI GCNGC 3 cut(s) 24, 279, 305
FriOI GRGCYC 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 24, 279, 305
GluI GCNGC 3 cut(s) 24, 279, 305
GsaI CCCAGC 1 cut(s) 416
HaeIII GGCC 3 cut(s) 51, 146, 393
HapII CCGG 1 cut(s) 72
HpaII CCGG 1 cut(s) 72
HphI GGTGA 3 cut(s) 167, 228, 280
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 5 cut(s) 113, 226, 255, 367, 434
Hpy188III TCNNGA 2 cut(s) 127, 316
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 2 cut(s) 382, 383
HpyCH4IV ACGT 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 20, 143
HpyF3I CTNAG 5 cut(s) 78, 110, 223, 350, 364
HpySE526I ACGT 1 cut(s) 421
Kzo9I GATC 2 cut(s) 184, 318
LmnI GCTCC 2 cut(s) 68, 286
LpnPI CCDG 8 cut(s) 48, 85, 112, 116, 130, 173, 186, 398
Lsp1109I GCAGC 3 cut(s) 35, 265, 291
LweI GCATC 2 cut(s) 14, 217
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 1 cut(s) 155
MalI GATC 2 cut(s) 186, 320
MboI GATC 2 cut(s) 184, 318
MboII GAAGA 1 cut(s) 242
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 395
MlsI TGGCCA 1 cut(s) 146
MluNI TGGCCA 1 cut(s) 146
MnlI CCTC 9 cut(s) 50, 77, 119, 175, 244, 258, 290, 320, 373
Mox20I TGGCCA 1 cut(s) 146
MscI TGGCCA 1 cut(s) 146
MseI TTAA 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 146
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 11, 20, 143
NciI CCSGG 1 cut(s) 73
NdeII GATC 2 cut(s) 184, 318
NlaIV GGNNCC 2 cut(s) 393, 417
NmuCI GTSAC 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 3 cut(s) 25, 280, 306
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 422
PspCI CACGTG 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 412
PspN4I GGNNCC 2 cut(s) 393, 417
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 3 cut(s) 391, 392, 416
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 3 cut(s) 24, 279, 305
Sau3AI GATC 2 cut(s) 184, 318
Sau96I GGNCC 3 cut(s) 391, 392, 416
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 2 cut(s) 14, 217
SinI GGWCC 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 179, 308
SmoI CTYRAG 2 cut(s) 179, 308
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 2 cut(s) 18, 52
TaiI ACGT 1 cut(s) 424
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 2 cut(s) 205, 364
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 3 cut(s) 23, 278, 304
Tsp45I GTSAC 1 cut(s) 155
TspRI CASTG 2 cut(s) 205, 364
VpaK11BI GGWCC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.