FvH4_6g32270

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012844
Physical Location & Seq
Forward (+)
25300368 .. 25301730
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32270.t1

Sequence Viewer

Length: 621 bp
ATGTTGAAAATGGAACAAAATAATCTACCTGTCATAGCCAAGAAACTATGGAGCATAGTACGTGTGGCTTTCTTCATGCTACGAAAAGGCATCTCAAAGCGGAAGCTCCTGCTAGACCTAAACATGATGATGAAGCGCGGCAAGCTGGCTAGCAAAGCCCTAAGCAATCTCATGTTCCACCACCACCACCACCACGGCGCCTCCGCCTCTGCCGCGGCCCAGCGCGAGTATGAGTTCAGCTGCAGCAACACCCCCAACTACCCCTTCCATCTCATGGGTGGCAAGCGCCGCAGCCACCACAGCAACCACTTCTTTAAGTGTGCCCACGCGCCTGCCACTCAAGAAGATGATGTGGCGGCAATGAATGCGGTTAAGGCAGTGCTGGAGATTCTGAATAATCATGAGGTGGTGGCGGCAGTGGAGGCGTCTCCGATGCCGACTCCATTTCAGACGCCTTCACTACTTCCCGGGTTTGGGCGGAGCCCCATGGTCCGCCAGCTGAGGATAACGGACTCACCGTTTCCGTTGAGGGAGACCGACGAGGACAGCCACGTGGACAAAGCGGCTGAGGAGTTCATTGAGAGGTTTTACAAGAATTTGAGACAGCAGAAGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

207

Amino Acids

23.5

Weight (kDa)

9.79

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 184 - 205 1.6e-06 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 274
AccII CGCG 4 cut(s) 138, 215, 225, 329
AcsI RAATTY 1 cut(s) 595
AcvI CACGTG 1 cut(s) 553
AcyI GRCGYC 3 cut(s) 198, 425, 452
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 274, 473, 474
AflIII ACRYGT 1 cut(s) 61
AgsI TTSAA 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 4 cut(s) 106, 145, 240, 499
AluI AGCT 4 cut(s) 106, 145, 240, 499
Alw26I GTCTC 3 cut(s) 432, 527, 595
Ama87I CYCGRG 1 cut(s) 467
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 240, 243, 291
ApoI RAATTY 1 cut(s) 595
AspLEI GCGC 5 cut(s) 138, 200, 225, 288, 331
AspS9I GGNCC 2 cut(s) 217, 490
AsuC2I CCSGG 2 cut(s) 468, 469
AsuHPI GGTGA 1 cut(s) 507
AsuNHI GCTAGC 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 485
BbrPI CACGTG 1 cut(s) 553
BbvCI CCTCAGC 2 cut(s) 500, 567
BbvI GCAGC 3 cut(s) 227, 255, 303
BccI CCATC 1 cut(s) 276
BceAI ACGGC 1 cut(s) 211
BcnI CCSGG 2 cut(s) 468, 469
BcoDI GTCTC 3 cut(s) 432, 527, 595
BfaI CTAG 2 cut(s) 113, 150
BfmI CTRYAG 1 cut(s) 241
BfoI RGCGCY 2 cut(s) 201, 289
Bme1390I CCNGG 2 cut(s) 468, 469
Bme18I GGWCC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 2 cut(s) 217, 490
BmiI GGNNCC 2 cut(s) 199, 482
BmrFI CCNGG 2 cut(s) 468, 469
BmsI GCATC 2 cut(s) 99, 423
BmtI GCTAGC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 404
Bpu10I CCTNAGC 3 cut(s) 161, 500, 567
BpuEI CTTGAG 1 cut(s) 324
BpuMI CCSGG 2 cut(s) 468, 469
BsaAI YACGTR 2 cut(s) 62, 553
BsaHI GRCGYC 3 cut(s) 198, 425, 452
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 4 cut(s) 193, 213, 467, 486
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 473, 474
Bse3DI GCAATG 1 cut(s) 366
BseDI CCNNGG 4 cut(s) 193, 213, 467, 486
BseLI CCNNNNNNNGG 3 cut(s) 274, 473, 474
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 491, 558
BseRI GAGGAG 1 cut(s) 584
BseSI GKGCMC 1 cut(s) 325
BseXI GCAGC 3 cut(s) 227, 255, 303
BseYI CCCAGC 1 cut(s) 219
Bsh1236I CGCG 4 cut(s) 138, 215, 225, 329
BshFI GGCC 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 467
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 274, 473, 474
BsmAI GTCTC 3 cut(s) 432, 527, 595
BsmBI CGTCTC 1 cut(s) 432
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 218
Bso31I GGTCTC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 325, 485
Bsp19I CCATGG 1 cut(s) 486
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 2 cut(s) 492, 559
BspFNI CGCG 4 cut(s) 138, 215, 225, 329
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 199, 482
BspMAI CTGCAG 1 cut(s) 245
BspOI GCTAGC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 197
BspTNI GGTCTC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 366
BssECI CCNNGG 4 cut(s) 193, 213, 467, 486
BssNI GRCGYC 3 cut(s) 198, 425, 452
BssT1I CCWWGG 1 cut(s) 486
Bst4CI ACNGT 1 cut(s) 519
BstACI GRCGYC 3 cut(s) 198, 425, 452
BstAPI GCANNNNNTGC 1 cut(s) 365
BstBAI YACGTR 2 cut(s) 62, 553
BstC8I GCNNGC 6 cut(s) 143, 147, 151, 284, 333, 497
BstDEI CTNAG 3 cut(s) 161, 500, 567
BstDSI CCRYGG 3 cut(s) 193, 213, 486
BstFNI CGCG 4 cut(s) 138, 215, 225, 329
BstH2I RGCGCY 2 cut(s) 201, 289
BstHHI GCGC 5 cut(s) 138, 200, 225, 288, 331
BstMAI GTCTC 3 cut(s) 432, 527, 595
BstMWI GCNNNNNNNGC 8 cut(s) 142, 155, 212, 288, 300, 365, 374, 422
BstSCI CCNGG 2 cut(s) 466, 467
BstSFI CTRYAG 1 cut(s) 241
BstSLI GKGCMC 1 cut(s) 325
BstUI CGCG 4 cut(s) 138, 215, 225, 329
BstV1I GCAGC 3 cut(s) 227, 255, 303
BsuRI GGCC 1 cut(s) 218
BtgI CCRYGG 3 cut(s) 193, 213, 486
BtsI GCAGTG 2 cut(s) 384, 423
BtsIMutI CAGTG 2 cut(s) 384, 423
Cac8I GCNNGC 6 cut(s) 143, 147, 151, 284, 333, 497
CciI TCATGA 1 cut(s) 400
CfoI GCGC 5 cut(s) 138, 200, 225, 288, 331
Cfr13I GGNCC 2 cut(s) 217, 490
Cfr42I CCGCGG 1 cut(s) 216
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 2 cut(s) 414, 460
Csp6I GTAC 1 cut(s) 59
CviAII CATG 6 cut(s) 76, 124, 172, 274, 401, 487
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 3 cut(s) 161, 500, 567
DinI GGCGCC 1 cut(s) 199
EciI GGCGGA 3 cut(s) 193, 482, 493
Eco130I CCWWGG 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 485
Eco31I GGTCTC 1 cut(s) 527
Eco47I GGWCC 1 cut(s) 490
Eco72I CACGTG 1 cut(s) 553
Eco88I CYCGRG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 485
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 432
FaeI CATG 6 cut(s) 79, 127, 175, 277, 404, 490
FatI CATG 6 cut(s) 75, 123, 171, 273, 400, 486
FriOI GRGCYC 1 cut(s) 485
FspBI CTAG 2 cut(s) 113, 150
GlaI GCGC 5 cut(s) 137, 199, 224, 287, 330
GsaI CCCAGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 2 cut(s) 201, 289
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 468
HgaI GACGC 2 cut(s) 414, 460
HhaI GCGC 5 cut(s) 138, 200, 225, 288, 331
Hin1I GRCGYC 3 cut(s) 198, 425, 452
Hin1II CATG 6 cut(s) 79, 127, 175, 277, 404, 490
Hin6I GCGC 5 cut(s) 136, 198, 223, 286, 329
HinP1I GCGC 5 cut(s) 136, 198, 223, 286, 329
HinfI GANTC 3 cut(s) 388, 439, 512
HpaII CCGG 1 cut(s) 468
HphI GGTGA 1 cut(s) 507
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 3 cut(s) 393, 432, 450
Hpy188III TCNNGA 2 cut(s) 341, 401
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 2 cut(s) 274, 465
HpyCH4III ACNGT 1 cut(s) 519
HpyCH4IV ACGT 2 cut(s) 61, 552
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 8 cut(s) 142, 155, 212, 288, 300, 365, 374, 422
HpyF3I CTNAG 3 cut(s) 161, 500, 567
HpySE526I ACGT 2 cut(s) 61, 552
Hsp92I GRCGYC 3 cut(s) 198, 425, 452
Hsp92II CATG 6 cut(s) 79, 127, 175, 277, 404, 490
HspAI GCGC 5 cut(s) 136, 198, 223, 286, 329
KasI GGCGCC 1 cut(s) 197
KspI CCGCGG 1 cut(s) 216
LmnI GCTCC 3 cut(s) 51, 111, 480
LpnPI CCDG 8 cut(s) 42, 122, 131, 233, 345, 368, 481, 509
Lsp1109I GCAGC 3 cut(s) 227, 255, 303
LweI GCATC 2 cut(s) 99, 423
MaeI CTAG 2 cut(s) 113, 150
MaeII ACGT 2 cut(s) 61, 552
MboII GAAGA 2 cut(s) 64, 356
MhlI GDGCHC 2 cut(s) 325, 485
MluCI AATT 1 cut(s) 595
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 433, 506
MnlI CCTC 9 cut(s) 211, 217, 397, 415, 495, 522, 535, 562, 576
MseI TTAA 2 cut(s) 315, 372
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 3 cut(s) 215, 240, 499
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 2 cut(s) 468, 469
Mva1269I GAATGC 1 cut(s) 370
MvnI CGCG 4 cut(s) 138, 215, 225, 329
MwoI GCNNNNNNNGC 8 cut(s) 142, 155, 212, 288, 300, 365, 374, 422
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 2 cut(s) 468, 469
NcoI CCATGG 1 cut(s) 486
NheI GCTAGC 1 cut(s) 149
NlaIII CATG 6 cut(s) 79, 127, 175, 277, 404, 490
NlaIV GGNNCC 2 cut(s) 199, 482
PagI TCATGA 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 515
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 274
PleI GAGTC 2 cut(s) 433, 506
PluTI GGCGCC 1 cut(s) 201
PmaCI CACGTG 1 cut(s) 553
PmlI CACGTG 1 cut(s) 553
PpsI GAGTC 2 cut(s) 433, 506
Ppu21I YACGTR 2 cut(s) 62, 553
PspCI CACGTG 1 cut(s) 553
PspFI CCCAGC 1 cut(s) 219
PspN4I GGNNCC 2 cut(s) 199, 482
PspPI GGNCC 2 cut(s) 217, 490
PstI CTGCAG 1 cut(s) 245
PvuII CAGCTG 2 cut(s) 240, 499
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 128
SacII CCGCGG 1 cut(s) 216
SaqAI TTAA 2 cut(s) 315, 372
Sau96I GGNCC 2 cut(s) 217, 490
SchI GAGTC 2 cut(s) 433, 506
ScrFI CCNGG 2 cut(s) 468, 469
SduI GDGCHC 2 cut(s) 325, 485
SfaNI GCATC 2 cut(s) 99, 423
SfcI CTRYAG 1 cut(s) 241
SfoI GGCGCC 1 cut(s) 199
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 1 cut(s) 490
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
Sse9I AATT 1 cut(s) 595
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 2 cut(s) 113, 150
StyD4I CCNGG 2 cut(s) 466, 467
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 1 cut(s) 519
TaiI ACGT 2 cut(s) 64, 555
TaqII GACCGA 1 cut(s) 551
TasI AATT 1 cut(s) 595
TauI GCSGC 7 cut(s) 141, 215, 218, 291, 359, 416, 566
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 2 cut(s) 315, 372
Tru9I TTAA 2 cut(s) 315, 372
TscAI CASTG 2 cut(s) 384, 423
TseI GCWGC 3 cut(s) 240, 243, 291
TspDTI ATGAA 4 cut(s) 64, 146, 377, 565
TspGWI ACGGA 2 cut(s) 513, 524
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 2 cut(s) 384, 423
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XmaI CCCGGG 1 cut(s) 467
XspI CTAG 2 cut(s) 113, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.