MD17G1185000.v1.1

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012844
Physical Location & Seq
Reverse (-)
21948691 .. 21949311
621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1185000.v1.1.491

Sequence Viewer

Length: 621 bp
ATGGAACAAAATAATCTACCAGTAATTGCTAGGAGAATATGGAGTATAGTACGTGTAGCTTTCTTCATGCTAAAAAAGGGCATTTCAAAGCGGAAGCTCCTACTCGACCTCAACATGATGATGAAGCGTGGCAAACTCGCCAGCAAAGCCCTAAGCAACCTCATGTTCCACCATCATCATCACAACCACTACCACCCCCACTACAGCAGCACCACCTCTTCTGCCGCCCCACAAGAGTATGAGTTCAGCTGCAGCAACACCCCCAACTACCCTTTTCACCTCATGGGCAAGCGCCGCCACCACAACAATCGCAACAACTTTTTCACATGTGCTCACGCGCAGCTAACTCAGGATGATGAGATGGCGACCGCAGGTGCCGTTAAGGCGGTTATGGAGATGTTGAATAATGATTTGTTGGCTGTGGAGACGGTGGCATCCGTGGCAGCATCACCGGTGCTTCCCGGGTTTGGAAAGAGCCCTATGGTCCGCCAACTAAGGATAACGGACTCACCATTTCCTTTGAGGGAGGCCGATGAGGATAGCCACGTAGATAAAGCGGCGGAGGAGTTTATAGAGAAGTTTTATAAGAATTTGTGGCAGCAGAGGAGAATGCATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

207

Amino Acids

23.77

Weight (kDa)

9.39

Isoelectric Point (pI)

60.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AarI CACCTGC 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 374
AccII CGCG 1 cut(s) 338
AciI CCGC 8 cut(s) 91, 225, 295, 369, 386, 487, 557, 560
AcsI RAATTY 1 cut(s) 589
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 467
AflIII ACRYGT 2 cut(s) 52, 326
AgeI ACCGGT 1 cut(s) 451
AgsI TTSAA 2 cut(s) 87, 403
AluBI AGCT 4 cut(s) 59, 97, 249, 343
AluI AGCT 4 cut(s) 59, 97, 249, 343
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 6 cut(s) 207, 249, 252, 340, 443, 598
ApoI RAATTY 1 cut(s) 589
AsiGI ACCGGT 1 cut(s) 451
AspLEI GCGC 2 cut(s) 294, 340
AspS9I GGNCC 1 cut(s) 484
AsuC2I CCSGG 2 cut(s) 462, 463
AsuHPI GGTGA 3 cut(s) 269, 441, 501
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 6 cut(s) 219, 236, 264, 352, 455, 610
BccI CCATC 2 cut(s) 180, 355
BceAI ACGGC 1 cut(s) 362
BcnI CCSGG 2 cut(s) 462, 463
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 2 cut(s) 202, 250
BfoI RGCGCY 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 9 cut(s) 208, 225, 250, 253, 295, 341, 444, 558, 599
BlsI GCNGC 9 cut(s) 209, 226, 251, 254, 296, 342, 445, 559, 600
Bme1390I CCNGG 2 cut(s) 462, 463
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 2 cut(s) 462, 463
BmsI GCATC 2 cut(s) 443, 455
Bpu10I CCTNAGC 1 cut(s) 152
BpuMI CCSGG 2 cut(s) 462, 463
BsaAI YACGTR 2 cut(s) 53, 547
BsaJI CCNNGG 2 cut(s) 438, 461
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 438, 461
BseGI GGATG 2 cut(s) 358, 434
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 2 cut(s) 578, 619
BseXI GCAGC 6 cut(s) 219, 236, 264, 352, 455, 610
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 530
BshNI GGYRCC 1 cut(s) 374
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 2 cut(s) 452, 462
BslI CCNNNNNNNGG 1 cut(s) 467
BsmAI GTCTC 1 cut(s) 419
BsmBI CGTCTC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 1 cut(s) 530
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 334, 479
BspACI CCGC 8 cut(s) 91, 225, 295, 369, 386, 487, 557, 560
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 254
BspMI ACCTGC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 2 cut(s) 438, 461
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 223
BstBAI YACGTR 2 cut(s) 53, 547
BstC8I GCNNGC 2 cut(s) 142, 290
BstDEI CTNAG 3 cut(s) 152, 348, 494
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 358, 434
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 294, 340
BstMAI GTCTC 1 cut(s) 419
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 146, 294, 383, 440
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 460, 461
BstSFI CTRYAG 2 cut(s) 202, 250
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 6 cut(s) 219, 236, 264, 352, 455, 610
BsuRI GGCC 1 cut(s) 530
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 2 cut(s) 358, 434
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 142, 290
CfoI GCGC 2 cut(s) 294, 340
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 484
Cfr9I CCCGGG 1 cut(s) 461
Csp6I GTAC 1 cut(s) 50
CspAI ACCGGT 1 cut(s) 451
CviAII CATG 6 cut(s) 67, 115, 163, 283, 327, 614
CviJI RGCY 9 cut(s) 59, 97, 149, 249, 343, 419, 477, 530, 543
CviKI_1 RGCY 9 cut(s) 59, 97, 149, 249, 343, 419, 477, 530, 543
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 3 cut(s) 152, 348, 494
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
EciI GGCGGA 2 cut(s) 476, 575
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 484
Eco88I CYCGRG 1 cut(s) 461
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 479
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 6 cut(s) 70, 118, 166, 286, 330, 617
FatI CATG 6 cut(s) 66, 114, 162, 282, 326, 613
Fnu4HI GCNGC 9 cut(s) 208, 225, 250, 253, 295, 341, 444, 558, 599
FokI GGATG 2 cut(s) 365, 421
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 9 cut(s) 208, 225, 250, 253, 295, 341, 444, 558, 599
FspBI CTAG 1 cut(s) 30
GlaI GCGC 2 cut(s) 293, 339
GluI GCNGC 9 cut(s) 208, 225, 250, 253, 295, 341, 444, 558, 599
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 530
HapII CCGG 2 cut(s) 452, 462
HhaI GCGC 2 cut(s) 294, 340
Hin1II CATG 6 cut(s) 70, 118, 166, 286, 330, 617
Hin6I GCGC 2 cut(s) 292, 338
HinP1I GCGC 2 cut(s) 292, 338
HinfI GANTC 1 cut(s) 506
HpaII CCGG 2 cut(s) 452, 462
HphI GGTGA 3 cut(s) 269, 441, 501
Hpy188III TCNNGA 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 2 cut(s) 52, 546
HpyCH4V TGCA 2 cut(s) 252, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 294, 383, 440
HpyF3I CTNAG 3 cut(s) 152, 348, 494
HpySE526I ACGT 2 cut(s) 52, 546
Hsp92II CATG 6 cut(s) 70, 118, 166, 286, 330, 617
HspAI GCGC 2 cut(s) 292, 338
LmnI GCTCC 1 cut(s) 102
LpnPI CCDG 6 cut(s) 33, 154, 335, 357, 465, 475
Lsp1109I GCAGC 6 cut(s) 219, 236, 264, 352, 455, 610
LweI GCATC 2 cut(s) 443, 455
MaeI CTAG 1 cut(s) 30
MaeII ACGT 2 cut(s) 52, 546
MboII GAAGA 2 cut(s) 55, 210
MhlI GDGCHC 2 cut(s) 334, 479
MluCI AATT 2 cut(s) 24, 589
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 9 cut(s) 119, 170, 226, 290, 516, 520, 529, 556, 597
Mph1103I ATGCAT 1 cut(s) 615
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 249
MspI CCGG 2 cut(s) 452, 462
MspR9I CCNGG 2 cut(s) 462, 463
Mva1269I GAATGC 1 cut(s) 615
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 146, 294, 383, 440
NciI CCSGG 2 cut(s) 462, 463
NlaIII CATG 6 cut(s) 70, 118, 166, 286, 330, 617
NlaIV GGNNCC 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 615
NspI RCATGY 1 cut(s) 330
PaqCI CACCTGC 1 cut(s) 362
PciI ACATGT 1 cut(s) 326
PctI GAATGC 1 cut(s) 615
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 9 cut(s) 209, 226, 251, 254, 296, 342, 445, 559, 600
PleI GAGTC 1 cut(s) 500
PpsI GAGTC 1 cut(s) 500
Ppu21I YACGTR 2 cut(s) 53, 547
PscI ACATGT 1 cut(s) 326
PsiI TTATAA 1 cut(s) 585
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 1 cut(s) 484
PstI CTGCAG 1 cut(s) 254
PvuII CAGCTG 1 cut(s) 249
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 9 cut(s) 208, 225, 250, 253, 295, 341, 444, 558, 599
Sau96I GGNCC 1 cut(s) 484
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 462, 463
SduI GDGCHC 2 cut(s) 334, 479
SfaNI GCATC 2 cut(s) 443, 455
SfcI CTRYAG 2 cut(s) 202, 250
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 1 cut(s) 484
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 119
Sse9I AATT 2 cut(s) 24, 589
SsiI CCGC 8 cut(s) 91, 225, 295, 369, 386, 487, 557, 560
SspMI CTAG 1 cut(s) 30
StyD4I CCNGG 2 cut(s) 460, 461
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 2 cut(s) 55, 549
TaqI TCGA 1 cut(s) 105
TasI AATT 2 cut(s) 24, 589
TauI GCSGC 3 cut(s) 227, 297, 560
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 6 cut(s) 207, 249, 252, 340, 443, 598
TspDTI ATGAA 2 cut(s) 55, 137
TspGWI ACGGA 2 cut(s) 427, 518
TspMI CCCGGG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 1 cut(s) 589
XceI RCATGY 1 cut(s) 330
XmaI CCCGGG 1 cut(s) 461
XspI CTAG 1 cut(s) 30
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.