Rh2DG431900

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012844
Physical Location & Seq
Forward (+)
62310648 .. 62311975
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG431900.1

Sequence Viewer

Length: 615 bp
ATGGAACAAAATAATCTACCCGTTATAGCCAAGAAACTATGGAGCATAGTACGTGTGGCTTTCTTCATGCTAAGAAAAGGCATCTCAAAGCGAAAGCTCCTGCTAGACCTCAACATGATGATGAAGCGCGGCAAGCTCGCCAGCAAAGCCCTGAGCAACCTCATGTTCCACCACCACCACGACGGCTCCTCCTCCGCTTCTTCAGCCGCCGCCGCCGCCGCCGCCCAGCGCGAGTATGAGTTCAGCTGCAGCAACACCCCCAACTACCGCTTCCATCTCATGAGTAGCAAGCGCCGCGGTCACCACAGCCACTTCTTTGCGTGCGCCCACGCGCCTGCCACTCAAGACGATGATGTGGCGGCGGTCAATGCCGTTAAGGCTGTGCTGGAGATTCTGAATAATCATGAGGTGGTGACAGCGGTGGAGGCGTCTCCGATGCCGACTCCATTTCAGACGCCTTCACTTCCAGGGTTCGGGCGGAGCCCAATGGTCCGCCAGCTGAGGATAACGGACTCACCGTTTCCGTTGAGGGAGGCCGACGAGGACAGCCACGTGGACAAAGCGGCGGAGGAGTTTATTGAGAGGTTTTACAAGAATTTGAGACAGCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

204

Amino Acids

22.94

Weight (kDa)

9.58

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 184 - 204 8.2e-06 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 129, 231, 297, 332
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 186
AcvI CACGTG 1 cut(s) 553
AcyI GRCGYC 2 cut(s) 428, 455
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 473
AflIII ACRYGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 4 cut(s) 97, 136, 246, 499
AluI AGCT 4 cut(s) 97, 136, 246, 499
Alw26I GTCTC 2 cut(s) 435, 595
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 2 cut(s) 246, 249
ApoI RAATTY 1 cut(s) 595
AspLEI GCGC 5 cut(s) 129, 231, 294, 326, 334
AspS9I GGNCC 1 cut(s) 490
AsuHPI GGTGA 3 cut(s) 293, 424, 507
AvaII GGWCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 485
BbrPI CACGTG 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 233, 261
BccI CCATC 1 cut(s) 282
BceAI ACGGC 2 cut(s) 199, 356
BciT130I CCWGG 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 435, 595
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 247
BfoI RGCGCY 1 cut(s) 295
BglI GCCNNNNNGGC 1 cut(s) 377
Bme1390I CCNGG 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 2 cut(s) 187, 482
BmrFI CCNGG 1 cut(s) 468
BmsI GCATC 2 cut(s) 90, 426
BpmI CTGGAG 1 cut(s) 407
Bpu10I CCTNAGC 2 cut(s) 152, 500
BpuEI CTTGAG 1 cut(s) 327
BsaAI YACGTR 2 cut(s) 53, 553
BsaHI GRCGYC 2 cut(s) 428, 455
BsaJI CCNNGG 2 cut(s) 295, 467
BsaXI ACNNNNNCTCC 4 cut(s) 170, 173, 200, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 473
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 295, 467
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMII CTCAG 2 cut(s) 143, 491
BseRI GAGGAG 3 cut(s) 178, 181, 584
BseXI GCAGC 2 cut(s) 233, 261
BseYI CCCAGC 1 cut(s) 225
Bsh1236I CGCG 4 cut(s) 129, 231, 297, 332
BshFI GGCC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 2 cut(s) 435, 595
BsmBI CGTCTC 1 cut(s) 435
BsnI GGCC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 485
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 2 cut(s) 144, 492
BspFNI CGCG 4 cut(s) 129, 231, 297, 332
BspHI TCATGA 2 cut(s) 279, 403
BspLI GGNNCC 2 cut(s) 187, 482
BspMAI CTGCAG 1 cut(s) 251
BssECI CCNNGG 2 cut(s) 295, 467
BssNI GRCGYC 2 cut(s) 428, 455
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 519
BstACI GRCGYC 2 cut(s) 428, 455
BstBAI YACGTR 2 cut(s) 53, 553
BstC8I GCNNGC 7 cut(s) 134, 138, 142, 290, 322, 336, 497
BstDEI CTNAG 3 cut(s) 71, 152, 500
BstDSI CCRYGG 1 cut(s) 295
BstEII GGTNACC 1 cut(s) 299
BstFNI CGCG 4 cut(s) 129, 231, 297, 332
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 5 cut(s) 129, 231, 294, 326, 334
BstMAI GTCTC 2 cut(s) 435, 595
BstNI CCWGG 1 cut(s) 468
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 4 cut(s) 129, 231, 297, 332
BstV1I GCAGC 2 cut(s) 233, 261
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 295
Cac8I GCNNGC 7 cut(s) 134, 138, 142, 290, 322, 336, 497
CciI TCATGA 2 cut(s) 279, 403
CfoI GCGC 5 cut(s) 129, 231, 294, 326, 334
Cfr13I GGNCC 1 cut(s) 490
Cfr42I CCGCGG 1 cut(s) 298
CseI GACGC 2 cut(s) 417, 463
Csp6I GTAC 1 cut(s) 50
CviAII CATG 5 cut(s) 67, 115, 163, 280, 404
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 3 cut(s) 71, 152, 500
EciI GGCGGA 3 cut(s) 482, 493, 581
Eco24I GRGCYC 1 cut(s) 485
Eco47I GGWCC 1 cut(s) 490
Eco57I CTGAAG 1 cut(s) 186
Eco72I CACGTG 1 cut(s) 553
Eco91I GGTNACC 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 485
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 5 cut(s) 70, 118, 166, 283, 407
FaiI YATR 9 cut(s) 26, 40, 47, 68, 116, 164, 237, 281, 405
FatI CATG 5 cut(s) 66, 114, 162, 279, 403
FriOI GRGCYC 1 cut(s) 485
FspBI CTAG 1 cut(s) 104
GlaI GCGC 5 cut(s) 128, 230, 293, 325, 333
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 536
HgaI GACGC 2 cut(s) 417, 463
HhaI GCGC 5 cut(s) 129, 231, 294, 326, 334
Hin1I GRCGYC 2 cut(s) 428, 455
Hin1II CATG 5 cut(s) 70, 118, 166, 283, 407
Hin6I GCGC 5 cut(s) 127, 229, 292, 324, 332
HinP1I GCGC 5 cut(s) 127, 229, 292, 324, 332
HinfI GANTC 3 cut(s) 391, 442, 512
HphI GGTGA 3 cut(s) 293, 424, 507
Hpy166II GTNNAC 1 cut(s) 556
Hpy188I TCNGA 3 cut(s) 396, 435, 453
Hpy188III TCNNGA 3 cut(s) 280, 344, 404
Hpy8I GTNNAC 1 cut(s) 556
Hpy99I CGWCG 2 cut(s) 185, 542
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 1 cut(s) 519
HpyCH4IV ACGT 2 cut(s) 52, 552
HpyCH4V TGCA 1 cut(s) 249
HpyF3I CTNAG 3 cut(s) 71, 152, 500
HpySE526I ACGT 2 cut(s) 52, 552
Hsp92I GRCGYC 2 cut(s) 428, 455
Hsp92II CATG 5 cut(s) 70, 118, 166, 283, 407
HspAI GCGC 5 cut(s) 127, 229, 292, 324, 332
KspI CCGCGG 1 cut(s) 298
LmnI GCTCC 4 cut(s) 42, 102, 191, 480
LpnPI CCDG 9 cut(s) 113, 154, 164, 239, 348, 371, 453, 480, 509
Lsp1109I GCAGC 2 cut(s) 233, 261
LweI GCATC 2 cut(s) 90, 426
MaeI CTAG 1 cut(s) 104
MaeII ACGT 2 cut(s) 52, 552
MaeIII GTNAC 2 cut(s) 299, 412
MboII GAAGA 2 cut(s) 55, 192
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 1 cut(s) 595
MlyI GAGTC 2 cut(s) 436, 506
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 4 cut(s) 246, 297, 419, 499
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 4 cut(s) 129, 231, 297, 332
NlaIII CATG 5 cut(s) 70, 118, 166, 283, 407
NlaIV GGNNCC 2 cut(s) 187, 482
NmuCI GTSAC 2 cut(s) 299, 412
PagI TCATGA 2 cut(s) 279, 403
PcsI WCGNNNNNNNCGW 1 cut(s) 515
PfeI GAWTC 1 cut(s) 391
PleI GAGTC 2 cut(s) 436, 506
PmaCI CACGTG 1 cut(s) 553
PmlI CACGTG 1 cut(s) 553
PpsI GAGTC 2 cut(s) 436, 506
Ppu21I YACGTR 2 cut(s) 53, 553
Psp6I CCWGG 1 cut(s) 466
PspCI CACGTG 1 cut(s) 553
PspEI GGTNACC 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 187, 482
PspPI GGNCC 1 cut(s) 490
PstI CTGCAG 1 cut(s) 251
PvuII CAGCTG 2 cut(s) 246, 499
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 119
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 1 cut(s) 375
Sau96I GGNCC 1 cut(s) 490
SchI GAGTC 2 cut(s) 436, 506
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 2 cut(s) 90, 426
SfcI CTRYAG 1 cut(s) 247
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 1 cut(s) 595
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 519
TaiI ACGT 2 cut(s) 55, 555
TasI AATT 1 cut(s) 595
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TseFI GTSAC 2 cut(s) 299, 412
TseI GCWGC 2 cut(s) 246, 249
Tsp45I GTSAC 2 cut(s) 299, 412
TspDTI ATGAA 2 cut(s) 55, 137
TspGWI ACGGA 2 cut(s) 513, 524
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 595
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.