MD09G1205200.v1.1

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012844
Physical Location & Seq
Reverse (-)
19554923 .. 19555534
612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1205200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGAACAAAATAATCTACCAGAAATTGCCAAGAGAATATGGAGTATAGTACGTGTAGCTTTCTTCATGCTAAGAAAGGGCATTTCAAAGCGAAAGCTCCTACTGGACTTCAACATGATGATGAAGCGTGGCAAGCTCGCCAGCAAAACCTTAAGCAATCTTATGTTCCACAACCATCACCATAGCCAAAGCCACCCCCACTACAGCTGGGCCACCTCCTCTGCCGCCCAACGAGAGTACGAGTTTAGCTGTAGCAACACCCCCAACTACCATTTTCACCTCATGGGCAAGCGCCGCCACCACAACCTCTTCAAGTGTACTCAAGTGCCGCAGACTCAGGATGATGAGATTGCGAGCGCAGGTGCCGTCACGGCAGTTTTGGAGATGTTGAACAATGAAGCGGTGACTGTGGAGGCAGCAGCATACGTGGCAGCATCACCGGTGCTTCCTGGGTTTGGGAAGAGCCCTATGGTCTGCCAGCTGAGGATAACGGACTCACCGTTTCCGTTGAGGGAGGCTGATGAGGAAAGCCACGTAGACAAAGCGGCGGAGGCGTTTATAGAGAAGTTCTATAAGAATTTGTGGCAACAGAGGAGAATGCATGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

204

Amino Acids

23.39

Weight (kDa)

8.98

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 537
AciI CCGC 6 cut(s) 225, 295, 329, 401, 545, 548
AcsI RAATTY 1 cut(s) 577
AfaI GTAC 3 cut(s) 51, 239, 319
AfiI CCNNNNNNNGG 1 cut(s) 455
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 52
AgeI ACCGGT 1 cut(s) 439
AgsI TTSAA 4 cut(s) 87, 112, 313, 391
AjnI CCWGG 1 cut(s) 448
AluBI AGCT 6 cut(s) 59, 97, 136, 207, 249, 481
AluI AGCT 6 cut(s) 59, 97, 136, 207, 249, 481
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 3 cut(s) 416, 419, 431
ApoI RAATTY 1 cut(s) 577
AsiGI ACCGGT 1 cut(s) 439
AspLEI GCGC 2 cut(s) 294, 359
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 5 cut(s) 170, 269, 415, 429, 489
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 467
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 428, 431, 443
BccI CCATC 1 cut(s) 183
BceAI ACGGC 2 cut(s) 350, 387
BciT130I CCWGG 1 cut(s) 450
BfmI CTRYAG 2 cut(s) 202, 250
BfoI RGCGCY 1 cut(s) 295
BfrI CTTAAG 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 371
BisI GCNGC 7 cut(s) 225, 295, 329, 417, 420, 432, 546
BlsI GCNGC 7 cut(s) 226, 296, 330, 418, 421, 433, 547
Bme1390I CCNGG 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 306
BsaAI YACGTR 3 cut(s) 53, 427, 535
BsaJI CCNNGG 1 cut(s) 449
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 449
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 350, 473
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 2 cut(s) 208, 607
BseXI GCAGC 3 cut(s) 428, 431, 443
BseYI CCCAGC 1 cut(s) 207
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 362
BshTI ACCGGT 1 cut(s) 439
BsiSI CCGG 1 cut(s) 440
BslI CCNNNNNNNGG 1 cut(s) 455
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 467
BspACI CCGC 6 cut(s) 225, 295, 329, 401, 545, 548
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 349, 474
BspLI GGNNCC 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 455
BspT107I GGYRCC 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 2 cut(s) 409, 501
Bst6I CTCTTC 2 cut(s) 314, 455
BstAFI CTTAAG 1 cut(s) 151
BstBAI YACGTR 3 cut(s) 53, 427, 535
BstC8I GCNNGC 6 cut(s) 134, 138, 142, 290, 355, 479
BstDEI CTNAG 3 cut(s) 71, 336, 482
BstF5I GGATG 1 cut(s) 346
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 294, 359
BstMWI GCNNNNNNNGC 5 cut(s) 133, 294, 371, 428, 551
BstNI CCWGG 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 448
BstSFI CTRYAG 2 cut(s) 202, 250
BstV1I GCAGC 3 cut(s) 428, 431, 443
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 346
BveI ACCTGC 1 cut(s) 350
Cac8I GCNNGC 6 cut(s) 134, 138, 142, 290, 355, 479
CfoI GCGC 2 cut(s) 294, 359
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 3 cut(s) 50, 238, 318
CspAI ACCGGT 1 cut(s) 439
CviAII CATG 4 cut(s) 67, 115, 283, 602
CviQI GTAC 3 cut(s) 50, 238, 318
DdeI CTNAG 3 cut(s) 71, 336, 482
Eam1104I CTCTTC 2 cut(s) 314, 455
EarI CTCTTC 2 cut(s) 314, 455
EciI GGCGGA 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 1 cut(s) 467
FaeI CATG 4 cut(s) 70, 118, 286, 605
FatI CATG 4 cut(s) 66, 114, 282, 601
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 7 cut(s) 225, 295, 329, 417, 420, 432, 546
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 7 cut(s) 225, 295, 329, 417, 420, 432, 546
GlaI GCGC 2 cut(s) 293, 358
GluI GCNGC 7 cut(s) 225, 295, 329, 417, 420, 432, 546
GsaI CCCAGC 1 cut(s) 211
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 212
HapII CCGG 1 cut(s) 440
HhaI GCGC 2 cut(s) 294, 359
Hin1II CATG 4 cut(s) 70, 118, 286, 605
Hin6I GCGC 2 cut(s) 292, 357
HinP1I GCGC 2 cut(s) 292, 357
HinfI GANTC 2 cut(s) 334, 494
HpaII CCGG 1 cut(s) 440
HphI GGTGA 5 cut(s) 170, 269, 415, 429, 489
Hpy166II GTNNAC 2 cut(s) 318, 538
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 2 cut(s) 318, 538
HpyCH4III ACNGT 2 cut(s) 409, 501
HpyCH4IV ACGT 3 cut(s) 52, 426, 534
HpyCH4V TGCA 1 cut(s) 601
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 294, 371, 428, 551
HpyF3I CTNAG 3 cut(s) 71, 336, 482
HpySE526I ACGT 3 cut(s) 52, 426, 534
Hsp92II CATG 4 cut(s) 70, 118, 286, 605
HspAI GCGC 2 cut(s) 292, 357
LguI GCTCTTC 1 cut(s) 455
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 428, 431, 443
LweI GCATC 1 cut(s) 443
MaeII ACGT 3 cut(s) 52, 426, 534
MaeIII GTNAC 2 cut(s) 367, 403
MboII GAAGA 3 cut(s) 55, 301, 472
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 2 cut(s) 24, 577
MlyI GAGTC 2 cut(s) 328, 488
Mph1103I ATGCAT 1 cut(s) 603
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 207, 481
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 5 cut(s) 133, 294, 371, 428, 551
NlaIII CATG 4 cut(s) 70, 118, 286, 605
NlaIV GGNNCC 1 cut(s) 364
NmuCI GTSAC 2 cut(s) 367, 403
NsiI ATGCAT 1 cut(s) 603
PaqCI CACCTGC 1 cut(s) 350
PciSI GCTCTTC 1 cut(s) 455
PcsI WCGNNNNNNNCGW 1 cut(s) 497
PctI GAATGC 1 cut(s) 603
PinAI ACCGGT 1 cut(s) 439
PkrI GCNGC 7 cut(s) 226, 296, 330, 418, 421, 433, 547
PleI GAGTC 2 cut(s) 328, 488
PpsI GAGTC 2 cut(s) 328, 488
Ppu21I YACGTR 3 cut(s) 53, 427, 535
Psp6I CCWGG 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 1 cut(s) 210
PvuII CAGCTG 2 cut(s) 207, 481
RsaI GTAC 3 cut(s) 51, 239, 319
RsaNI GTAC 3 cut(s) 50, 238, 318
RseI CAYNNNNRTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 7 cut(s) 225, 295, 329, 417, 420, 432, 546
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 2 cut(s) 328, 488
ScrFI CCNGG 1 cut(s) 450
SduI GDGCHC 1 cut(s) 467
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 2 cut(s) 202, 250
SgrAI CRCCGGYG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 151, 321
SmoI CTYRAG 2 cut(s) 151, 321
Sse9I AATT 2 cut(s) 24, 577
SsiI CCGC 6 cut(s) 225, 295, 329, 401, 545, 548
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 409, 501
TaiI ACGT 3 cut(s) 55, 429, 537
TasI AATT 2 cut(s) 24, 577
TatI WGTACW 1 cut(s) 317
TauI GCSGC 4 cut(s) 227, 297, 331, 548
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TseFI GTSAC 2 cut(s) 367, 403
TseI GCWGC 3 cut(s) 416, 419, 431
Tsp45I GTSAC 2 cut(s) 367, 403
TspDTI ATGAA 3 cut(s) 55, 137, 411
TspGWI ACGGA 2 cut(s) 495, 506
Vha464I CTTAAG 1 cut(s) 151
XapI RAATTY 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 537
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.