Rw2G033780

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012844
Physical Location & Seq
Forward (+)
55159433 .. 55160400
968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033780.1

Sequence Viewer

Length: 618 bp
ATGGAACAAAATAATCTACCCGTTATAGCCAAGAAACTATGGAGCATAGTACGTGTGGCTTTCTTCATGCTAAGAAAAGGCATCTCAAAGCGAAAGCTCCTGCTAGACCTCAACATGATGATGAAGCGCGGCAAGCTCGCCAGCAAAGCCCTGAGCAACCTCATGTTCCACCACCACCACGACGGCTCCTCCTCCGCTTCTTCAGCCTCCGCCGCCGCCCAGCGCGAGTATGAGTTCAGCTGCAGCAACACCCCCAACTACCGCTTCCATCTCATGAGTGGCAAGCGCCGCGGTCACCACAGCAACCACAGCCACTTCTTTGCGTGCGCTCACGCGCCTGCCACTCAAGAAGATGATGTGGCGGCGGTCAATGCCGTTAAGGCTGTGCTGGAGATTCTGAATAATCATGAGGTGGTGACAGCGGTGGAGGCGTCTCCGATGTCGACTCCATTTCAGACGCCTTCACTTCCAGGGTTCGGGCGGAGCCCAATGGTCCGCCAGCTGAGGATAACGGACTCACCGTTTCCGTTGAGGGAGGCCGACGAGGACAGCCACGTGGACAAAGCGGCGGAGGAGTTTATTGAGAGGTTTTACAAGAATTTGAGACAGCAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

205

Amino Acids

23.13

Weight (kDa)

9.58

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 185 - 205 8.3e-06 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 443
AccII CGCG 4 cut(s) 129, 225, 291, 335
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 186
AcvI CACGTG 1 cut(s) 556
AcyI GRCGYC 2 cut(s) 431, 458
AfaI GTAC 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 476
AflIII ACRYGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 469
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
AluBI AGCT 4 cut(s) 97, 136, 240, 502
AluI AGCT 4 cut(s) 97, 136, 240, 502
Alw26I GTCTC 2 cut(s) 438, 598
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 2 cut(s) 240, 243
ApoI RAATTY 1 cut(s) 598
AspLEI GCGC 5 cut(s) 129, 225, 288, 329, 337
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 3 cut(s) 287, 427, 510
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 488
BbrPI CACGTG 1 cut(s) 556
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 227, 255
BccI CCATC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 199, 359
BciT130I CCWGG 1 cut(s) 471
BcoDI GTCTC 2 cut(s) 438, 598
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 289
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 8 cut(s) 130, 213, 216, 241, 244, 289, 363, 567
BlsI GCNGC 8 cut(s) 131, 214, 217, 242, 245, 290, 364, 568
Bme1390I CCNGG 1 cut(s) 471
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 2 cut(s) 187, 485
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 2 cut(s) 152, 503
BpuEI CTTGAG 1 cut(s) 330
BsaAI YACGTR 2 cut(s) 53, 556
BsaHI GRCGYC 2 cut(s) 431, 458
BsaJI CCNNGG 2 cut(s) 289, 470
BsaXI ACNNNNNCTCC 4 cut(s) 170, 173, 200, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 476
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 289, 470
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMII CTCAG 2 cut(s) 143, 494
BseRI GAGGAG 3 cut(s) 178, 181, 587
BseXI GCAGC 2 cut(s) 227, 255
BseYI CCCAGC 1 cut(s) 219
Bsh1236I CGCG 4 cut(s) 129, 225, 291, 335
BshFI GGCC 1 cut(s) 539
BslI CCNNNNNNNGG 1 cut(s) 476
BsmAI GTCTC 2 cut(s) 438, 598
BsmBI CGTCTC 1 cut(s) 438
BsnI GGCC 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 488
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 2 cut(s) 144, 495
BspFNI CGCG 4 cut(s) 129, 225, 291, 335
BspHI TCATGA 2 cut(s) 273, 406
BspLI GGNNCC 2 cut(s) 187, 485
BspMAI CTGCAG 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 289, 470
BssNI GRCGYC 2 cut(s) 431, 458
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 522
BstACI GRCGYC 2 cut(s) 431, 458
BstBAI YACGTR 2 cut(s) 53, 556
BstC8I GCNNGC 7 cut(s) 134, 138, 142, 284, 325, 339, 500
BstDEI CTNAG 3 cut(s) 71, 152, 503
BstDSI CCRYGG 1 cut(s) 289
BstEII GGTNACC 1 cut(s) 293
BstFNI CGCG 4 cut(s) 129, 225, 291, 335
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 5 cut(s) 129, 225, 288, 329, 337
BstMAI GTCTC 2 cut(s) 438, 598
BstMWI GCNNNNNNNGC 9 cut(s) 133, 146, 203, 212, 288, 309, 371, 380, 428
BstNI CCWGG 1 cut(s) 471
BstPI GGTNACC 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 469
BstSFI CTRYAG 1 cut(s) 241
BstUI CGCG 4 cut(s) 129, 225, 291, 335
BstV1I GCAGC 2 cut(s) 227, 255
BsuRI GGCC 1 cut(s) 539
BtgI CCRYGG 1 cut(s) 289
Cac8I GCNNGC 7 cut(s) 134, 138, 142, 284, 325, 339, 500
CciI TCATGA 2 cut(s) 273, 406
CfoI GCGC 5 cut(s) 129, 225, 288, 329, 337
Cfr13I GGNCC 1 cut(s) 493
Cfr42I CCGCGG 1 cut(s) 292
CseI GACGC 2 cut(s) 420, 466
Csp6I GTAC 1 cut(s) 50
CviAII CATG 5 cut(s) 67, 115, 163, 274, 407
CviQI GTAC 1 cut(s) 50
DdeI CTNAG 3 cut(s) 71, 152, 503
EciI GGCGGA 4 cut(s) 199, 485, 496, 584
Eco24I GRGCYC 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 186
Eco72I CACGTG 1 cut(s) 556
Eco91I GGTNACC 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 488
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 5 cut(s) 70, 118, 166, 277, 410
FaiI YATR 9 cut(s) 26, 40, 47, 68, 116, 164, 231, 275, 408
FatI CATG 5 cut(s) 66, 114, 162, 273, 406
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 8 cut(s) 130, 213, 216, 241, 244, 289, 363, 567
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 8 cut(s) 130, 213, 216, 241, 244, 289, 363, 567
FspBI CTAG 1 cut(s) 104
GlaI GCGC 5 cut(s) 128, 224, 287, 328, 336
GluI GCNGC 8 cut(s) 130, 213, 216, 241, 244, 289, 363, 567
GsaI CCCAGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 289
HaeIII GGCC 1 cut(s) 539
HgaI GACGC 2 cut(s) 420, 466
HhaI GCGC 5 cut(s) 129, 225, 288, 329, 337
Hin1I GRCGYC 2 cut(s) 431, 458
Hin1II CATG 5 cut(s) 70, 118, 166, 277, 410
Hin6I GCGC 5 cut(s) 127, 223, 286, 327, 335
HinP1I GCGC 5 cut(s) 127, 223, 286, 327, 335
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 3 cut(s) 394, 445, 515
HphI GGTGA 3 cut(s) 287, 427, 510
Hpy166II GTNNAC 2 cut(s) 444, 559
Hpy188I TCNGA 3 cut(s) 399, 438, 456
Hpy188III TCNNGA 3 cut(s) 274, 347, 407
Hpy8I GTNNAC 2 cut(s) 444, 559
Hpy99I CGWCG 2 cut(s) 185, 545
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 1 cut(s) 522
HpyCH4IV ACGT 2 cut(s) 52, 555
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 146, 203, 212, 288, 309, 371, 380, 428
HpyF3I CTNAG 3 cut(s) 71, 152, 503
HpySE526I ACGT 2 cut(s) 52, 555
Hsp92I GRCGYC 2 cut(s) 431, 458
Hsp92II CATG 5 cut(s) 70, 118, 166, 277, 410
HspAI GCGC 5 cut(s) 127, 223, 286, 327, 335
KspI CCGCGG 1 cut(s) 292
LmnI GCTCC 4 cut(s) 42, 102, 191, 483
LpnPI CCDG 9 cut(s) 113, 154, 164, 233, 351, 374, 456, 483, 512
Lsp1109I GCAGC 2 cut(s) 227, 255
LweI GCATC 1 cut(s) 90
MaeI CTAG 1 cut(s) 104
MaeII ACGT 2 cut(s) 52, 555
MaeIII GTNAC 2 cut(s) 293, 415
MboII GAAGA 3 cut(s) 55, 192, 362
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 1 cut(s) 598
MlyI GAGTC 2 cut(s) 439, 509
MseI TTAA 1 cut(s) 378
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 4 cut(s) 240, 291, 422, 502
MspR9I CCNGG 1 cut(s) 471
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 4 cut(s) 129, 225, 291, 335
MwoI GCNNNNNNNGC 9 cut(s) 133, 146, 203, 212, 288, 309, 371, 380, 428
NlaIII CATG 5 cut(s) 70, 118, 166, 277, 410
NlaIV GGNNCC 2 cut(s) 187, 485
NmuCI GTSAC 2 cut(s) 293, 415
PagI TCATGA 2 cut(s) 273, 406
PcsI WCGNNNNNNNCGW 1 cut(s) 518
PfeI GAWTC 1 cut(s) 394
PkrI GCNGC 8 cut(s) 131, 214, 217, 242, 245, 290, 364, 568
PleI GAGTC 2 cut(s) 439, 509
PmaCI CACGTG 1 cut(s) 556
PmlI CACGTG 1 cut(s) 556
PpsI GAGTC 2 cut(s) 439, 509
Ppu21I YACGTR 2 cut(s) 53, 556
Psp6I CCWGG 1 cut(s) 469
PspCI CACGTG 1 cut(s) 556
PspEI GGTNACC 1 cut(s) 293
PspFI CCCAGC 1 cut(s) 219
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 187, 485
PspPI GGNCC 1 cut(s) 493
PstI CTGCAG 1 cut(s) 245
PvuII CAGCTG 2 cut(s) 240, 502
RsaI GTAC 1 cut(s) 51
RsaNI GTAC 1 cut(s) 50
RseI CAYNNNNRTG 1 cut(s) 119
SacII CCGCGG 1 cut(s) 292
SalI GTCGAC 1 cut(s) 442
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 8 cut(s) 130, 213, 216, 241, 244, 289, 363, 567
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 2 cut(s) 439, 509
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 1 cut(s) 488
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 241
Sfr303I CCGCGG 1 cut(s) 292
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
Sse9I AATT 1 cut(s) 598
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 522
TaiI ACGT 2 cut(s) 55, 558
TaqI TCGA 1 cut(s) 443
TasI AATT 1 cut(s) 598
TauI GCSGC 6 cut(s) 132, 215, 218, 291, 365, 569
TfiI GAWTC 1 cut(s) 394
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TseFI GTSAC 2 cut(s) 293, 415
TseI GCWGC 2 cut(s) 240, 243
Tsp45I GTSAC 2 cut(s) 293, 415
TspDTI ATGAA 2 cut(s) 55, 137
TspGWI ACGGA 2 cut(s) 516, 527
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.