pycom09g12180

Avr9 Cf-9 rapidly elicited protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012844
Physical Location & Seq
Reverse (-)
10633053 .. 10633676
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12180.1

Sequence Viewer

Length: 624 bp
ATGGAACAAAATAATCTACCAGAAATTGCCAAGAGAATATGGAGAGTAGTACGTGTAGCTTTCTTCATGCTAAGAAAGGGCATTTCAAAGCGAAAGCTCCTACTGGACTTCAACATGATGATGAAGCGTGGCAAGCTCGCCAGCAAAACCTTAAGCAATCTCATGTTCCACAGCCACCACCACAGCCACAGCCACCCCCACTACAGCGGGGCCACCTCCTCTGCCGCCCAACGAGAGTACGAGTTCAGTTGCAGCAACACCCCCAACTACCCTTTTCACCTCAGGGGCAAGCGCTGCCACGACAACAACCACCACAACCTCTTCAAGTGTACTCACGCGCTGCAGACTCAGGATGATGAGATGGCGACCGCAGGTGCCGTCACGGCGGTTTTGGAGATGTTGAACAATGAAGCAGTGACTGTGGAGGCGGCGGCATACGTGGCAACATCACCGGTGCTTCCCGGGTTTGGGAAGAGCCCTATGGTCCGCCAGCTGAGGATAACGGACTCACCGTTTCCGGTGAGGGAGGCTGATGAGGAAAGCCACGTGGACAAAGCGGCGGAGGAGTTTATAGAGAAGTTCTATAAGAATTTGTGGCAACAGAGGAGAATGCATGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

208

Amino Acids

23.85

Weight (kDa)

8.98

Isoelectric Point (pI)

54.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52140 AT3G16330
fragaria_vesca FvH4_6g32270
malus_domestica MD09G1205200.v1.1 MD17G1185000.v1.1
prunus_persica Prupe.3G060500_v2.0.a1
pyrus_communis pycom09g12180 pycom17g19490
rosa_chinensis RchiOBHm_Chr2g0140151
rosa_laevigata RLG00000019856
rosa_multiflora Rmu_sc0003244.1_g000017
rosa_roxburghii Rroxscaffold_2G00105000
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412200 Rh2BG422600 Rh2CG398300 Rh2DG431900
rosa_wichuraiana Rw2G033780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
Acc36I ACCTGC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 374
AccII CGCG 1 cut(s) 338
AciI CCGC 9 cut(s) 207, 225, 369, 386, 428, 431, 487, 557, 560
AcsI RAATTY 1 cut(s) 589
AcvI CACGTG 1 cut(s) 547
AfaI GTAC 3 cut(s) 51, 239, 331
AfeI AGCGCT 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 467, 468
AflII CTTAAG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 52
AgeI ACCGGT 1 cut(s) 451
AgsI TTSAA 4 cut(s) 87, 112, 325, 403
AluBI AGCT 4 cut(s) 59, 97, 136, 493
AluI AGCT 4 cut(s) 59, 97, 136, 493
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 461
Aor51HI AGCGCT 1 cut(s) 293
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 3 cut(s) 252, 294, 340
ApoI RAATTY 1 cut(s) 589
AsiGI ACCGGT 1 cut(s) 451
AspLEI GCGC 2 cut(s) 294, 340
AspS9I GGNCC 2 cut(s) 210, 484
AsuC2I CCSGG 2 cut(s) 462, 463
AsuHPI GGTGA 4 cut(s) 269, 441, 501, 532
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 484
AxyI CCTNAGG 1 cut(s) 281
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 3 cut(s) 264, 281, 327
BccI CCATC 1 cut(s) 355
BceAI ACGGC 2 cut(s) 362, 399
BcnI CCSGG 2 cut(s) 462, 463
BfmI CTRYAG 2 cut(s) 202, 341
BfoI RGCGCY 1 cut(s) 295
BfrI CTTAAG 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 362
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 7 cut(s) 225, 253, 295, 341, 429, 432, 558
BlsI GCNGC 7 cut(s) 226, 254, 296, 342, 430, 433, 559
Bme1390I CCNGG 2 cut(s) 462, 463
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 210, 484
BmiI GGNNCC 2 cut(s) 211, 376
BmrFI CCNGG 2 cut(s) 462, 463
Bpu10I CCTNAGC 1 cut(s) 494
BpuMI CCSGG 2 cut(s) 462, 463
BsaAI YACGTR 3 cut(s) 53, 439, 547
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 2 cut(s) 451, 517
Bsc4I CCNNNNNNNGG 2 cut(s) 467, 468
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 108
Bse21I CCTNAGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 467, 468
BseMII CTCAG 3 cut(s) 295, 362, 485
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 3 cut(s) 208, 578, 619
BseXI GCAGC 3 cut(s) 264, 281, 327
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 374
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 452, 462, 518
BslI CCNNNNNNNGG 2 cut(s) 467, 468
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 479
BspACI CCGC 9 cut(s) 207, 225, 369, 386, 428, 431, 487, 557, 560
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 3 cut(s) 294, 361, 486
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 211, 376
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 461
Bst4CI ACNGT 2 cut(s) 421, 513
Bst6I CTCTTC 2 cut(s) 326, 467
BstAFI CTTAAG 1 cut(s) 151
BstAPI GCANNNNNTGC 1 cut(s) 294
BstBAI YACGTR 3 cut(s) 53, 439, 547
BstC8I GCNNGC 5 cut(s) 134, 138, 142, 290, 491
BstDEI CTNAG 4 cut(s) 71, 281, 348, 494
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 2 cut(s) 294, 340
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 133, 294, 383, 440
BstSCI CCNGG 2 cut(s) 460, 461
BstSFI CTRYAG 2 cut(s) 202, 341
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 3 cut(s) 264, 281, 327
Bsu36I CCTNAGG 1 cut(s) 281
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 5 cut(s) 134, 138, 142, 290, 491
CaiI CAGNNNCTG 1 cut(s) 419
CfoI GCGC 2 cut(s) 294, 340
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 210, 484
Cfr9I CCCGGG 1 cut(s) 461
Csp6I GTAC 3 cut(s) 50, 238, 330
CspAI ACCGGT 1 cut(s) 451
CviAII CATG 4 cut(s) 67, 115, 163, 614
CviQI GTAC 3 cut(s) 50, 238, 330
DdeI CTNAG 4 cut(s) 71, 281, 348, 494
Eam1104I CTCTTC 2 cut(s) 326, 467
EarI CTCTTC 2 cut(s) 326, 467
EciI GGCGGA 2 cut(s) 476, 575
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 484
Eco47III AGCGCT 1 cut(s) 293
Eco72I CACGTG 1 cut(s) 547
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 461
EcoT22I ATGCAT 1 cut(s) 615
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 4 cut(s) 70, 118, 166, 617
FaiI YATR 9 cut(s) 40, 68, 116, 164, 436, 482, 572, 585, 615
FatI CATG 4 cut(s) 66, 114, 162, 613
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 7 cut(s) 225, 253, 295, 341, 429, 432, 558
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 7 cut(s) 225, 253, 295, 341, 429, 432, 558
GlaI GCGC 2 cut(s) 293, 339
GluI GCNGC 7 cut(s) 225, 253, 295, 341, 429, 432, 558
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 452, 462, 518
HhaI GCGC 2 cut(s) 294, 340
Hin1II CATG 4 cut(s) 70, 118, 166, 617
Hin6I GCGC 2 cut(s) 292, 338
HinP1I GCGC 2 cut(s) 292, 338
HinfI GANTC 2 cut(s) 346, 506
HpaII CCGG 3 cut(s) 452, 462, 518
HphI GGTGA 4 cut(s) 269, 441, 501, 532
Hpy166II GTNNAC 2 cut(s) 330, 550
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 2 cut(s) 330, 550
HpyCH4III ACNGT 2 cut(s) 421, 513
HpyCH4IV ACGT 3 cut(s) 52, 438, 546
HpyCH4V TGCA 3 cut(s) 252, 343, 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 294, 383, 440
HpyF3I CTNAG 4 cut(s) 71, 281, 348, 494
HpySE526I ACGT 3 cut(s) 52, 438, 546
Hsp92II CATG 4 cut(s) 70, 118, 166, 617
HspAI GCGC 2 cut(s) 292, 338
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 264, 281, 327
MaeII ACGT 3 cut(s) 52, 438, 546
MaeIII GTNAC 2 cut(s) 379, 415
MboII GAAGA 3 cut(s) 55, 313, 484
MhlI GDGCHC 1 cut(s) 479
MluCI AATT 2 cut(s) 24, 589
MlyI GAGTC 2 cut(s) 340, 500
Mph1103I ATGCAT 1 cut(s) 615
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 207, 493
MspCI CTTAAG 1 cut(s) 151
MspI CCGG 3 cut(s) 452, 462, 518
MspR9I CCNGG 2 cut(s) 462, 463
Mva1269I GAATGC 1 cut(s) 615
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 4 cut(s) 133, 294, 383, 440
NciI CCSGG 2 cut(s) 462, 463
NlaIII CATG 4 cut(s) 70, 118, 166, 617
NlaIV GGNNCC 2 cut(s) 211, 376
NmuCI GTSAC 2 cut(s) 379, 415
NsiI ATGCAT 1 cut(s) 615
PaqCI CACCTGC 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 509
PctI GAATGC 1 cut(s) 615
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 7 cut(s) 226, 254, 296, 342, 430, 433, 559
PleI GAGTC 2 cut(s) 340, 500
PmaCI CACGTG 1 cut(s) 547
PmlI CACGTG 1 cut(s) 547
PpsI GAGTC 2 cut(s) 340, 500
Ppu21I YACGTR 3 cut(s) 53, 439, 547
PspCI CACGTG 1 cut(s) 547
PspN4I GGNNCC 2 cut(s) 211, 376
PspPI GGNCC 2 cut(s) 210, 484
PstI CTGCAG 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 3 cut(s) 51, 239, 331
RsaNI GTAC 3 cut(s) 50, 238, 330
RseI CAYNNNNRTG 1 cut(s) 119
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 7 cut(s) 225, 253, 295, 341, 429, 432, 558
Sau96I GGNCC 2 cut(s) 210, 484
SchI GAGTC 2 cut(s) 340, 500
ScrFI CCNGG 2 cut(s) 462, 463
SduI GDGCHC 1 cut(s) 479
SfcI CTRYAG 2 cut(s) 202, 341
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 1 cut(s) 484
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 2 cut(s) 24, 589
SsiI CCGC 9 cut(s) 207, 225, 369, 386, 428, 431, 487, 557, 560
StyD4I CCNGG 2 cut(s) 460, 461
TaaI ACNGT 2 cut(s) 421, 513
TaiI ACGT 3 cut(s) 55, 441, 549
TasI AATT 2 cut(s) 24, 589
TatI WGTACW 1 cut(s) 329
TauI GCSGC 4 cut(s) 227, 431, 434, 560
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 2 cut(s) 379, 415
TseI GCWGC 3 cut(s) 252, 294, 340
Tsp45I GTSAC 2 cut(s) 379, 415
TspDTI ATGAA 3 cut(s) 55, 137, 423
TspGWI ACGGA 1 cut(s) 518
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 1 cut(s) 420
Vha464I CTTAAG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 1 cut(s) 589
XmaI CCCGGG 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.