Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008604

Physical Location & Seq

Genomic Coordinates Forward (+)

25435529 .. 25442859

Gene Structure 7331 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g32381.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2082 bp

ATGGCTTCATACAGGCCTTTCCCGCCTCAACCGGCGTTTCCGCCGCCGCCGCCGAATCAAAACCCCATCGCGCCACCACCACGTGGCAATCAGTACAACCAGAATTGGGGTTATAATGCCGCCGCCGGCGGCGATGGGGCTGCTCCTTCCTCGTTTCCCCAGAACTACATTCACCACCCCCATGGTAATTATGGTCCTCCTAGAGCTCAACACCCTCACCCACCGCCGCAGTACTCGTACCCGCCACCGCCTCCTCCGCCGGACTCTTCATACCCGCCACCACCGCCTCCTCAAGCTCCTCCCGGGAAGAATTTGCCGCTACAGCCTCCGCAGGCGCCAATGTACTACCAGTCTAACTCTCAATATGCGCAGTATGGTCACCAGGCAATGCAGCCGCCACCGCCTCCCCCGCCACCTTTGTCTCCTAGTTTGGCTATACCACCGCCGCCCCCGCCTCCAAATTCTCCACCACCTCCGCCACCGCCGAAGAGTAGGGAGAGTGGTTTTGATAAGGGGCCGCATGACAAAGGAGCTTCCAAGGATGTGGGAGCTTCTGCCAAGCGTGAACATGGGCATTCAAACCATCATGGGGTTCCTCCTAAACACAAGCCGCCTGTTCCATTGGTGAAGAAGTCGAATGGGGCGCCCGGGAGAGTGGAGACAGAGGAAGAGAGGAGGTTGAGGAAGAAGAGGGAGTTTGAGAAGCAAAGACAAGAGGAGAAGCATAGACAGCAGGCAAAGGAGTCCCAAAATTCGGTTTTGCAAAAGACGCATTTGATGTCTTCCGGGAAGGGTCATGGATCGATTGCAGGGTCGCGAATGGGGGAAAGGAGGACCACTCCATTCTTGAGTGGTGAGAGGGCTGAAAATAGGCTAAAGAAGCCAACCACATTTGTCTGCAAGTTGAAATTCCGGAACGAACTTCCAGATCCAAGTGCACAACCAAAACTGATGTCCATGAAGAAGGACCCAGACCAGTATACAAAATACACAATTACATCGTTAGAGAAAAATTACAAGCCGAAGCTTTTTGTTGAGCCAGATCTTGGGATACCTCTTGACCTTCTTGACCTCAGTGTATACAATCCTCCTCCCGGTCCTAGACCACCACTTGCACCAGAAGACGAAGAGTTATTGCGTGATGATGTGGCAGTGACCCCTGTTAAAAAAGATGGCATCAGAAGAAAAGAACGACCTACAGACAAAGGAGTTGCATGGCTGGTTAAAACACAATATATTTCTCCTCTTAGCATGGATTCAGCTAAGCAGTCTCTAACTGAAAAACAAGCAAAAGAGCTGCGGGAAATGAAGGGAGGACGCAATCTTTTGGACAACCTTAATGATAGAGAAAGGCAGATTAAGGAAATCGAGGCATCGTTTGAGGCCTGCAAGTCACGCCCTGTTCATGCAACCAATAAGAATCTATACCCTGTTGAGGTTCTACCTCTTTTGCCTGATTTCGAGCGGTATGAAGACCAATTCGTCCTTGCCGGTTTTGATGGTGCTCCAACCGCTGATTCAGAAATCTACAGCAAGTTGGATCAGTCAGATCATGATCTTTGTGAATCAAGGGCAATTATGAAAAGTTACAAGGTAACAGGCGCAGATCCAGCTAATCCTGACAAATTTTTGGCATACATGGTCCCTTCGCCAAATGAGCTATCAAAGGATCCGTATGATGAATCTGAAGATATTTCATATTCTTGGGTTCGGGAGTATCAGTATGATGTAAGAGGTGATGATGTCGATGATCTGACAACATATCTTGTATCATTCGATGAAGATGCAGCACGATATGCGCCGCTTCCAGCAAAGCTTGTTTTGAGGAAAAAAAGGGCGAAAGAGGGACGATCCACTGATGAGGTTGAACATTTTCCTGCACCTTCAAGAGTGACTGTCAGGCGGAGATCGACTGTTTCTGCAATTGAACTAAAGGATGCAGGGGATTATTCAAGGGGAGCTCTATCAAATTTGAAAAGGAGAGGGTTTGATAATGAAGATGCTCTTGAAAGACCACAGAAGAGGGGACGACACCAAGATGTTGATGAATATAGTGGCGCTGAAGATGATTTATCTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009909 GO:0009910 GO:0009987 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0019538 GO:0030880 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0051093 GO:0051239 GO:0051241 GO:0051276 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:1901564 GO:1902494 GO:1990234 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04011 map04011

Pfam Domains

Protein Families

Paf1

Protein Analysis

694

Amino Acids

77.5

Weight (kDa)

8.48

Isoelectric Point (pI)

62.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Paf1	PF03985	298 - 636	2.5e-57	Paf1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79730
fragaria_vesca	FvH4_6g32381 FvH4_6g32381
malus_domestica	MD09G1202900.v1.1 MD17G1184000.v1.1
prunus_persica	Prupe.1G178600_v2.0.a1 Prupe.1G178600_v2.0.a1 Prupe.1G178600_v2.0.a1 Prupe.1G178600_v2.0.a1 Prupe.3G059100_v2.0.a1
pyrus_communis	pycom17g19340
rosa_chinensis	RchiOBHm_Chr2g0140531
rosa_laevigata	RLG00000019875
rosa_multiflora	Rmu_sc0002490.1_g000024
rosa_roxburghii	Rroxscaffold_153G00436660 Rroxscaffold_2G00104720
rosa_rugosa	Rorug02G0363400 Rorug02G0363500
rosa_samantha	Rh2AG414300 Rh2BG424200 Rh2CG400700 Rh2DG434200
rosa_wichuraiana	Rw2G033930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	114
AasI	GACNNNNNNGTC	1 cut(s)	1481
Acc16I	TGCGCA	1 cut(s)	369
AccB1I	GGYRCC	2 cut(s)	334, 643
AccB7I	CCANNNNNTGG	2 cut(s)	83, 1046
AccBSI	CCGCTC	1 cut(s)	1465
AccI	GTMKAC	2 cut(s)	980, 1080
AccII	CGCG	2 cut(s)	71, 817
AccIII	TCCGGA	1 cut(s)	912
AclWI	GGATC	7 cut(s)	808, 923, 1548, 1601, 1664, 1677, 1845
AcsI	RAATTY	6 cut(s)	310, 460, 751, 908, 1625, 1969
AcuI	CTGAAG	2 cut(s)	1707, 2082
AcvI	CACGTG	1 cut(s)	83
AcyI	GRCGYC	2 cut(s)	335, 644
AdeI	CACNNNGTG	1 cut(s)	83
AfaI	GTAC	4 cut(s)	95, 233, 239, 344
AfiI	CCNNNNNNNGG	7 cut(s)	83, 106, 589, 754, 1046, 1094, 1435
AgsI	TTSAA	8 cut(s)	579, 907, 1868, 1887, 1928, 1953, 1975, 2009
AjnI	CCWGG	1 cut(s)	381
AjuI	GAANNNNNNNTTGG	2 cut(s)	1501, 1533
Alw21I	GWGCWC	4 cut(s)	208, 940, 1507, 1963
Alw26I	GTCTC	3 cut(s)	426, 653, 1275
Alw44I	GTGCAC	1 cut(s)	936
AlwI	GGATC	7 cut(s)	808, 923, 1548, 1601, 1664, 1677, 1845
Ama87I	CYCGRG	2 cut(s)	302, 647
Aor13HI	TCCGGA	1 cut(s)	912
AoxI	GGCC	3 cut(s)	14, 515, 1383
ApaLI	GTGCAC	1 cut(s)	936
ApeKI	GCWGC	4 cut(s)	140, 391, 1297, 1787
ApoI	RAATTY	6 cut(s)	310, 460, 751, 908, 1625, 1969
ArsI	GACNNNNNNTTYG	2 cut(s)	938, 970
Asp700I	GAANNNNTTC	1 cut(s)	1872
AspLEI	GCGC	7 cut(s)	73, 337, 370, 646, 1604, 1801, 2060
AspS9I	GGNCC	6 cut(s)	194, 515, 834, 967, 1097, 1642
AsuC2I	CCSGG	6 cut(s)	303, 304, 648, 649, 787, 1095
AsuHPI	GGTGA	6 cut(s)	164, 209, 371, 637, 866, 1748
AvaI	CYCGRG	2 cut(s)	302, 647
AvaII	GGWCC	5 cut(s)	194, 834, 967, 1097, 1642
BaeGI	GKGCMC	1 cut(s)	940
BamHI	GGATCC	1 cut(s)	1669
BanI	GGYRCC	2 cut(s)	334, 643
BanII	GRGCYC	2 cut(s)	208, 1963
BbrPI	CACGTG	1 cut(s)	83
BbsI	GAAGAC	3 cut(s)	774, 1128, 1479
Bbv12I	GWGCWC	4 cut(s)	208, 940, 1507, 1963
BbvI	GCAGC	4 cut(s)	127, 403, 1284, 1799
BccI	CCATC	5 cut(s)	74, 128, 591, 1166, 1493
BcgI	CGANNNNNNTGC	4 cut(s)	122, 156, 1766, 1800
BciT130I	CCWGG	1 cut(s)	383
BciVI	GTATCC	1 cut(s)	1044
BcnI	CCSGG	6 cut(s)	303, 304, 648, 649, 787, 1095
BcoDI	GTCTC	3 cut(s)	426, 653, 1275
BfaI	CTAG	3 cut(s)	201, 426, 1101
BfmI	CTRYAG	3 cut(s)	320, 1197, 1528
BfoI	RGCGCY	3 cut(s)	338, 647, 2061
BfuI	GTATCC	1 cut(s)	1044
BglII	AGATCT	1 cut(s)	1042
BlpI	GCTNAGC	1 cut(s)	1263
BmcAI	AGTACT	1 cut(s)	233
Bme1390I	CCNGG	7 cut(s)	303, 304, 383, 648, 649, 787, 1095
Bme18I	GGWCC	5 cut(s)	194, 834, 967, 1097, 1642
BmeT110I	CYCGRG	2 cut(s)	302, 647
BmgT120I	GGNCC	6 cut(s)	194, 515, 834, 967, 1097, 1642
BmiI	GGNNCC	7 cut(s)	336, 516, 594, 645, 969, 1644, 1671
BmrFI	CCNGG	7 cut(s)	303, 304, 383, 648, 649, 787, 1095
BmsI	GCATC	5 cut(s)	1185, 1382, 1774, 1927, 1990
BpiI	GAAGAC	3 cut(s)	774, 1128, 1479
BplI	GAGNNNNNCTC	2 cut(s)	823, 855
Bpu1102I	GCTNAGC	1 cut(s)	1263
BpuEI	CTTGAG	2 cut(s)	276, 868
BpuMI	CCSGG	6 cut(s)	303, 304, 648, 649, 787, 1095
Bsa29I	ATCGAT	1 cut(s)	803
BsaAI	YACGTR	1 cut(s)	83
BsaBI	GATNNNNATC	1 cut(s)	1554
BsaHI	GRCGYC	2 cut(s)	335, 644
BsaJI	CCNNGG	4 cut(s)	181, 302, 537, 647
BsaWI	WCCGGW	1 cut(s)	912
BsaXI	ACNNNNNCTCC	2 cut(s)	1706, 1736
Bsc4I	CCNNNNNNNGG	7 cut(s)	83, 106, 589, 754, 1046, 1094, 1435
Bse118I	RCCGGY	3 cut(s)	31, 125, 1490
Bse1I	ACTGG	2 cut(s)	349, 976
Bse3DI	GCAATG	1 cut(s)	393
Bse8I	GATNNNNATC	1 cut(s)	1554
BseAI	TCCGGA	1 cut(s)	912
BseBI	CCWGG	1 cut(s)	383
BseCI	ATCGAT	1 cut(s)	803
BseDI	CCNNGG	4 cut(s)	181, 302, 537, 647
BseGI	GGATG	2 cut(s)	547, 1942
BseJI	GATNNNNATC	1 cut(s)	1554
BseLI	CCNNNNNNNGG	7 cut(s)	83, 106, 589, 754, 1046, 1094, 1435
BseMI	GCAATG	1 cut(s)	393
BseMII	CTCAG	1 cut(s)	1087
BseNI	ACTGG	2 cut(s)	349, 976
BseRI	GAGGAG	7 cut(s)	243, 279, 288, 688, 731, 1080, 1233
BseSI	GKGCMC	1 cut(s)	940
BseXI	GCAGC	4 cut(s)	127, 403, 1284, 1799
BsgI	GTGCAG	1 cut(s)	1863
Bsh1236I	CGCG	2 cut(s)	71, 817
BshFI	GGCC	3 cut(s)	16, 517, 1385
BshNI	GGYRCC	2 cut(s)	334, 643
BshVI	ATCGAT	1 cut(s)	803
BsiHKAI	GWGCWC	4 cut(s)	208, 940, 1507, 1963
BsiHKCI	CYCGRG	2 cut(s)	302, 647
BsiSI	CCGG	9 cut(s)	32, 126, 260, 303, 648, 786, 913, 1095, 1491
BslFI	GGGAC	4 cut(s)	730, 1628, 1860, 2040
BslI	CCNNNNNNNGG	7 cut(s)	83, 106, 589, 754, 1046, 1094, 1435
BsmAI	GTCTC	3 cut(s)	426, 653, 1275
BsmFI	GGGAC	4 cut(s)	730, 1628, 1860, 2040
BsmI	GAATGC	1 cut(s)	574
BsnI	GGCC	3 cut(s)	16, 517, 1385
BsoBI	CYCGRG	2 cut(s)	302, 647
Bsp1286I	GDGCHC	4 cut(s)	208, 940, 1507, 1963
Bsp13I	TCCGGA	1 cut(s)	912
Bsp1720I	GCTNAGC	1 cut(s)	1263
Bsp19I	CCATGG	1 cut(s)	181
Bsp68I	TCGCGA	1 cut(s)	817
BspANI	GGCC	3 cut(s)	16, 517, 1385
BspCNI	CTCAG	1 cut(s)	1086
BspDI	ATCGAT	1 cut(s)	803
BspEI	TCCGGA	1 cut(s)	912
BspFNI	CGCG	2 cut(s)	71, 817
BspHI	TCATGA	1 cut(s)	1552
BspLI	GGNNCC	7 cut(s)	336, 516, 594, 645, 969, 1644, 1671
BspPI	GGATC	7 cut(s)	808, 923, 1548, 1601, 1664, 1677, 1845
BspT107I	GGYRCC	2 cut(s)	334, 643
BsrBI	CCGCTC	1 cut(s)	1465
BsrDI	GCAATG	1 cut(s)	393
BsrFI	RCCGGY	3 cut(s)	31, 125, 1490
BsrI	ACTGG	2 cut(s)	349, 976
BssAI	RCCGGY	3 cut(s)	31, 125, 1490
BssECI	CCNNGG	4 cut(s)	181, 302, 537, 647
BssNAI	GTATAC	2 cut(s)	981, 1081
BssNI	GRCGYC	2 cut(s)	335, 644
BssT1I	CCWWGG	2 cut(s)	181, 537
Bst1107I	GTATAC	2 cut(s)	981, 1081
Bst2UI	CCWGG	1 cut(s)	383
Bst4CI	ACNGT	2 cut(s)	1897, 1915
Bst6I	CTCTTC	6 cut(s)	271, 482, 663, 683, 1122, 2015
BstACI	GRCGYC	2 cut(s)	335, 644
BstAPI	GCANNNNNTGC	1 cut(s)	1796
BstBAI	YACGTR	1 cut(s)	83
BstC8I	GCNNGC	4 cut(s)	127, 333, 735, 1387
BstDEI	CTNAG	3 cut(s)	1073, 1247, 1263
BstDSI	CCRYGG	1 cut(s)	181
BstEII	GGTNACC	1 cut(s)	377
BstF5I	GGATG	2 cut(s)	547, 1942
BstFNI	CGCG	2 cut(s)	71, 817
BstH2I	RGCGCY	3 cut(s)	338, 647, 2061
BstHHI	GCGC	7 cut(s)	73, 337, 370, 646, 1604, 1801, 2060
BstMAI	GTCTC	3 cut(s)	426, 653, 1275
BstNI	CCWGG	1 cut(s)	383
BstPI	GGTNACC	1 cut(s)	377
BstSCI	CCNGG	7 cut(s)	301, 302, 381, 646, 647, 785, 1093
BstSFI	CTRYAG	3 cut(s)	320, 1197, 1528
BstSLI	GKGCMC	1 cut(s)	940
BstUI	CGCG	2 cut(s)	71, 817
BstV1I	GCAGC	4 cut(s)	127, 403, 1284, 1799
BstV2I	GAAGAC	3 cut(s)	774, 1128, 1479
BstX2I	RGATCY	4 cut(s)	928, 1042, 1606, 1669
BstXI	CCANNNNNNTGG	2 cut(s)	182, 544
BstYI	RGATCY	4 cut(s)	928, 1042, 1606, 1669
BstZ17I	GTATAC	2 cut(s)	981, 1081
Bsu15I	ATCGAT	1 cut(s)	803
BsuI	GTATCC	1 cut(s)	1044
BsuRI	GGCC	3 cut(s)	16, 517, 1385
BsuTUI	ATCGAT	1 cut(s)	803
BtgI	CCRYGG	1 cut(s)	181
BtgZI	GCGATG	2 cut(s)	52, 147
BtsCI	GGATG	2 cut(s)	547, 1942
BtsI	GCAGTG	1 cut(s)	1158
BtsIMutI	CAGTG	3 cut(s)	1081, 1158, 1854
BtuMI	TCGCGA	1 cut(s)	817
Cac8I	GCNNGC	4 cut(s)	127, 333, 735, 1387
CciI	TCATGA	1 cut(s)	1552
CfoI	GCGC	7 cut(s)	73, 337, 370, 646, 1604, 1801, 2060
Cfr10I	RCCGGY	3 cut(s)	31, 125, 1490
Cfr13I	GGNCC	6 cut(s)	194, 515, 834, 967, 1097, 1642
Cfr9I	CCCGGG	2 cut(s)	302, 647
ClaI	ATCGAT	1 cut(s)	803
CseI	GACGC	2 cut(s)	778, 1326
Csp6I	GTAC	4 cut(s)	94, 232, 238, 343
CviQI	GTAC	4 cut(s)	94, 232, 238, 343
DdeI	CTNAG	3 cut(s)	1073, 1247, 1263
DinI	GGCGCC	2 cut(s)	336, 645
DraIII	CACNNNGTG	1 cut(s)	83
DrdI	GACNNNNNNGTC	1 cut(s)	1481
DseDI	GACNNNNNNGTC	1 cut(s)	1481
Eam1104I	CTCTTC	6 cut(s)	271, 482, 663, 683, 1122, 2015
EarI	CTCTTC	6 cut(s)	271, 482, 663, 683, 1122, 2015
EciI	GGCGGA	4 cut(s)	30, 246, 465, 1918
Ecl136II	GAGCTC	2 cut(s)	206, 1961
Eco130I	CCWWGG	2 cut(s)	181, 537
Eco147I	AGGCCT	2 cut(s)	16, 1385
Eco24I	GRGCYC	2 cut(s)	208, 1963
Eco47I	GGWCC	5 cut(s)	194, 834, 967, 1097, 1642
Eco53kI	GAGCTC	2 cut(s)	206, 1961
Eco57I	CTGAAG	2 cut(s)	1707, 2082
Eco72I	CACGTG	1 cut(s)	83
Eco88I	CYCGRG	2 cut(s)	302, 647
Eco91I	GGTNACC	1 cut(s)	377
EcoICRI	GAGCTC	2 cut(s)	206, 1961
EcoO109I	RGGNCCY	1 cut(s)	967
EcoO65I	GGTNACC	1 cut(s)	377
EcoRII	CCWGG	1 cut(s)	381
EcoT14I	CCWWGG	2 cut(s)	181, 537
EcoT38I	GRGCYC	2 cut(s)	208, 1963
EgeI	GGCGCC	2 cut(s)	336, 645
EheI	GGCGCC	2 cut(s)	336, 645
ErhI	CCWWGG	2 cut(s)	181, 537
FalI	AAGNNNNNCTT	2 cut(s)	1989, 2021
FaqI	GGGAC	4 cut(s)	730, 1628, 1860, 2040
FauI	CCCGC	6 cut(s)	30, 249, 282, 417, 459, 1293
FblI	GTMKAC	2 cut(s)	980, 1080
FokI	GGATG	2 cut(s)	554, 1949
FriOI	GRGCYC	2 cut(s)	208, 1963
FspBI	CTAG	3 cut(s)	201, 426, 1101
FspI	TGCGCA	1 cut(s)	369
GlaI	GCGC	7 cut(s)	72, 336, 369, 645, 1603, 1800, 2059
HaeII	RGCGCY	3 cut(s)	338, 647, 2061
HaeIII	GGCC	3 cut(s)	16, 517, 1385
HapII	CCGG	9 cut(s)	32, 126, 260, 303, 648, 786, 913, 1095, 1491
HgaI	GACGC	2 cut(s)	778, 1326
HhaI	GCGC	7 cut(s)	73, 337, 370, 646, 1604, 1801, 2060
Hin1I	GRCGYC	2 cut(s)	335, 644
Hin6I	GCGC	7 cut(s)	71, 335, 368, 644, 1602, 1799, 2058
HinP1I	GCGC	7 cut(s)	71, 335, 368, 644, 1602, 1799, 2058
HindIII	AAGCTT	2 cut(s)	1025, 1814
HinfI	GANTC	8 cut(s)	55, 263, 743, 1256, 1420, 1517, 1565, 1682
HpaII	CCGG	9 cut(s)	32, 126, 260, 303, 648, 786, 913, 1095, 1491
HphI	GGTGA	6 cut(s)	164, 209, 371, 637, 866, 1748
Hpy166II	GTNNAC	4 cut(s)	566, 938, 981, 1081
Hpy188I	TCNGA	6 cut(s)	1181, 1522, 1549, 1687, 1755, 2077
Hpy8I	GTNNAC	4 cut(s)	566, 938, 981, 1081
HpyAV	CCTTC	7 cut(s)	156, 784, 958, 1073, 1303, 1656, 1893
HpyCH4III	ACNGT	2 cut(s)	1897, 1915
HpyCH4IV	ACGT	1 cut(s)	82
HpyF3I	CTNAG	3 cut(s)	1073, 1247, 1263
HpySE526I	ACGT	1 cut(s)	82
Hsp92I	GRCGYC	2 cut(s)	335, 644
HspAI	GCGC	7 cut(s)	71, 335, 368, 644, 1602, 1799, 2058
KasI	GGCGCC	2 cut(s)	334, 643
Kpn2I	TCCGGA	1 cut(s)	912
KroI	GCCGGC	1 cut(s)	125
KroNI	GCCGGC	1 cut(s)	127
LmnI	GCTCC	6 cut(s)	148, 301, 530, 548, 1510, 1958
Lsp1109I	GCAGC	4 cut(s)	127, 403, 1284, 1799
LweI	GCATC	5 cut(s)	1185, 1382, 1774, 1927, 1990
MaeI	CTAG	3 cut(s)	201, 426, 1101
MaeII	ACGT	1 cut(s)	82
MaeIII	GTNAC	6 cut(s)	377, 1153, 1392, 1586, 1594, 1891
MbiI	CCGCTC	1 cut(s)	1465
MfeI	CAATTG	1 cut(s)	1923
MflI	RGATCY	4 cut(s)	928, 1042, 1606, 1669
MhlI	GDGCHC	4 cut(s)	208, 940, 1507, 1963
Mly113I	GGCGCC	2 cut(s)	335, 644
MlyI	GAGTC	2 cut(s)	257, 752
MmeI	TCCRAC	2 cut(s)	1518, 1532
MreI	CGCCGGCG	1 cut(s)	125
MroI	TCCGGA	1 cut(s)	912
MroNI	GCCGGC	1 cut(s)	125
MroXI	GAANNNNTTC	1 cut(s)	1872
MseI	TTAA	4 cut(s)	1164, 1224, 1338, 1359
MslI	CAYNNNNRTG	2 cut(s)	180, 2037
MspA1I	CMGCKG	1 cut(s)	1514
MspI	CCGG	9 cut(s)	32, 126, 260, 303, 648, 786, 913, 1095, 1491
MspR9I	CCNGG	7 cut(s)	303, 304, 383, 648, 649, 787, 1095
MunI	CAATTG	1 cut(s)	1923
Mva1269I	GAATGC	1 cut(s)	574
MvaI	CCWGG	1 cut(s)	383
MvnI	CGCG	2 cut(s)	71, 817
NaeI	GCCGGC	1 cut(s)	127
NarI	GGCGCC	2 cut(s)	335, 644
NciI	CCSGG	6 cut(s)	303, 304, 648, 649, 787, 1095
NcoI	CCATGG	1 cut(s)	181
NgoMIV	GCCGGC	1 cut(s)	125
NlaIV	GGNNCC	7 cut(s)	336, 516, 594, 645, 969, 1644, 1671
NmuCI	GTSAC	4 cut(s)	377, 1153, 1392, 1891
NruI	TCGCGA	1 cut(s)	817
NsbI	TGCGCA	1 cut(s)	369
PagI	TCATGA	1 cut(s)	1552
PceI	AGGCCT	2 cut(s)	16, 1385
PctI	GAATGC	1 cut(s)	574
PdiI	GCCGGC	1 cut(s)	127
PdmI	GAANNNNTTC	1 cut(s)	1872
PfeI	GAWTC	6 cut(s)	55, 1256, 1420, 1517, 1565, 1682
PflMI	CCANNNNNTGG	2 cut(s)	83, 1046
PfoI	TCCNGGA	1 cut(s)	785
PleI	GAGTC	2 cut(s)	257, 751
PluTI	GGCGCC	2 cut(s)	338, 647
PmaCI	CACGTG	1 cut(s)	83
PmlI	CACGTG	1 cut(s)	83
PpsI	GAGTC	2 cut(s)	257, 751
Ppu21I	YACGTR	1 cut(s)	83
PpuMI	RGGWCCY	1 cut(s)	967
PsiI	TTATAA	1 cut(s)	114
Psp124BI	GAGCTC	2 cut(s)	208, 1963
Psp5II	RGGWCCY	1 cut(s)	967
Psp6I	CCWGG	1 cut(s)	381
PspCI	CACGTG	1 cut(s)	83
PspEI	GGTNACC	1 cut(s)	377
PspGI	CCWGG	1 cut(s)	381
PspN4I	GGNNCC	7 cut(s)	336, 516, 594, 645, 969, 1644, 1671
PspPI	GGNCC	6 cut(s)	194, 515, 834, 967, 1097, 1642
PspPPI	RGGWCCY	1 cut(s)	967
PsuI	RGATCY	4 cut(s)	928, 1042, 1606, 1669
RruI	TCGCGA	1 cut(s)	817
RsaI	GTAC	4 cut(s)	95, 233, 239, 344
RsaNI	GTAC	4 cut(s)	94, 232, 238, 343
RseI	CAYNNNNRTG	2 cut(s)	180, 2037
SacI	GAGCTC	2 cut(s)	208, 1963
SaqAI	TTAA	4 cut(s)	1164, 1224, 1338, 1359
Sau96I	GGNCC	6 cut(s)	194, 515, 834, 967, 1097, 1642
ScaI	AGTACT	1 cut(s)	233
SchI	GAGTC	2 cut(s)	257, 752
ScrFI	CCNGG	7 cut(s)	303, 304, 383, 648, 649, 787, 1095
SduI	GDGCHC	4 cut(s)	208, 940, 1507, 1963
SfaNI	GCATC	5 cut(s)	1185, 1382, 1774, 1927, 1990
SfcI	CTRYAG	3 cut(s)	320, 1197, 1528
SfoI	GGCGCC	2 cut(s)	336, 645
SgrAI	CRCCGGYG	1 cut(s)	125
SinI	GGWCC	5 cut(s)	194, 834, 967, 1097, 1642
SmaI	CCCGGG	2 cut(s)	304, 649
SmiMI	CAYNNNNRTG	2 cut(s)	180, 2037
SmlI	CTYRAG	2 cut(s)	291, 847
SmoI	CTYRAG	2 cut(s)	291, 847
SseBI	AGGCCT	2 cut(s)	16, 1385
SspDI	GGCGCC	2 cut(s)	334, 643
SspMI	CTAG	3 cut(s)	201, 426, 1101
SstI	GAGCTC	2 cut(s)	208, 1963
StuI	AGGCCT	2 cut(s)	16, 1385
StyD4I	CCNGG	7 cut(s)	301, 302, 381, 646, 647, 785, 1093
StyI	CCWWGG	2 cut(s)	181, 537
TaaI	ACNGT	2 cut(s)	1897, 1915
TaiI	ACGT	1 cut(s)	85
TaqI	TCGA	7 cut(s)	635, 803, 1368, 1461, 1746, 1776, 1910
TatI	WGTACW	3 cut(s)	93, 231, 342
TfiI	GAWTC	6 cut(s)	55, 1256, 1420, 1517, 1565, 1682
Tru1I	TTAA	4 cut(s)	1164, 1224, 1338, 1359
Tru9I	TTAA	4 cut(s)	1164, 1224, 1338, 1359
TscAI	CASTG	3 cut(s)	1081, 1158, 1861
TseFI	GTSAC	4 cut(s)	377, 1153, 1392, 1891
TseI	GCWGC	4 cut(s)	140, 391, 1297, 1787
Tsp45I	GTSAC	4 cut(s)	377, 1153, 1392, 1891
TspGWI	ACGGA	1 cut(s)	1662
TspMI	CCCGGG	2 cut(s)	302, 647
TspRI	CASTG	3 cut(s)	1081, 1158, 1861
Van91I	CCANNNNNTGG	2 cut(s)	83, 1046
VneI	GTGCAC	1 cut(s)	936
VpaK11BI	GGWCC	5 cut(s)	194, 834, 967, 1097, 1642
XapI	RAATTY	6 cut(s)	310, 460, 751, 908, 1625, 1969
XcmI	CCANNNNNNNNNTGG	1 cut(s)	188
XmaI	CCCGGG	2 cut(s)	302, 647
XmiI	GTMKAC	2 cut(s)	980, 1080
XmnI	GAANNNNTTC	1 cut(s)	1872
XspI	CTAG	3 cut(s)	201, 426, 1101
ZrmI	AGTACT	1 cut(s)	233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.