FvH4_6g32880

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006221
Physical Location & Seq
Forward (+)
25949149 .. 25955187
6039 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32880.t1

Sequence Viewer

Length: 1983 bp
ATGAACGTTGTTGAGGAGGAAGAGAGTGCTTTTGAGATTGGGATTGTGGTTCCAAAGAGAAATGTTAAGGCGGAAGAAGAGGATTACTGTGTGGAGGTTTTGGTCAATGAATTCAAGAATGTGGGTTTTATAGTTGAGAGAGTCCCTGGCGTTGTTGATGACTTCATCAAGCTGGCAGCGCCTTTGGAGACATTGGGGAGAGCTGCAGTCGAGTTGCAAATCAAAAAGCGAACTCGAATTGGGATGGATTTACAATTTGAATGGGACGAGGTTGATGCTTTTGTGAGGCAACCTGATGGTTCATTGTTCAGCTGGTGTGAGCGTTTTCATTGCTATAGGCACTTGATATATGGAATTATGAACAAGAGCAAGTCAGCTGTAACCCTGAGATTTGATGGGAAAGAGTTCAATTGGGAAGTTGGGGAATATTTACTTAAGAAATTGGAGTCACAGAGGATTGTCAAAGAAGTTTTTGCTTTGCATGATGAAGTGAAAAGGAAGAAACTCCTTCACACTTGGGCTTTGCACTGGTGGGACTTCACAAACCAGCCCATAGATGAGATTTATTCATATTTTGGTACAAAGATTGCCATCTACTTCGCTTTCCTTGGAATGTATACACGGTGGTTGCTGTTTCCAGCTACATTTGGGCTTATTCTGCAGATATTTGATTTTGGGTCATTGCAGTTACTGGTGCTGCCCCTTTTCTTTATAAGCATAACATTATGGGCCATAATGTTTTCCCAGTTCTGGAAACGCAAGAATGTTGCTCTTTTAGCTAGATGGCACATCAGTTATCCAATTAGAACCGACCTGGGAAATAGGCAGCTGGGCACAGCTTGGAATTCGCCGCAATCATCTGTTGAAATCATGAAGAAATGGGGAACAGACAAAACAAAAGAGAAAGAAATGTTTCAAAGAATTGAGTGGTTTGGACGACTAATGAGATTCCGAAATGATGCTATCATTATAGTTAGTATTATATGCCTCCAGTTACCCTTTGAGCTGGCATATGCTCATCTTTACGAGGTTATTGAGTCTGACATTGTGAAGTTTGGGTTGACAACCGTATATCTGTTTGCTATTCAGTTTTTTACGCAGCTCGGGGGCAAGATCTCTGTGAAGCTTATTAATTACGAGAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTTTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATACATAGGAGTCTTCTACCATGCTCTTATACATCGCAACTTTTTGACACTTCGCACAGTTTTGATTCAGCGCCTCCTTGTGTCTGAGGTATTGGAAAACTTGGTGGAAAATACTTTGCCATATTTGAAGTACAGTTATAAGAAATTTAGAGTACGAAGCAAGAAGAAACGTGAAAAGGGATCATCAACAGGGAGAATCCAGTTTACTTCTAGGGTGGAGAAGGAGTACTTGAAGCCTTCTTATTCTGCAAGCATTGGTATGGAGCTTGAAGATGGACTTTTCGATGACTTTCTAGAGTTGACATTGCAGTTTGGTATGATCATGATGTTTGCTTGTGCACTCCCCCCTGCTTTTGCCTTTGCAGCTGTGAACAACATAACTGAAATTAGGACAGATGCATTGAAGCTGCTCGTCATGCATAAGAGGCCAATTCCTCGTGTTTCCACAACAATTGGTGCTTGGCTAAACATATTTCAGTTTCTTGTAGTTACATCTATATGCACCAACTGCGCCCTTCTAGTATGGTTATATGATCATGAGGGTAAATGGAATATAGAGCCTGGCCTAGCTGCAATTCTGGTTATGGAACATGTTCTCCTGTTGATCAAGTTTTGTTTCTCTCGCTTTGTTCCCGAGGAACCTGCTTGGGTACAGGCGAACCGTGTGAAGAAAGCAACAGAGGCCGAGTACATGTGCTCAAAACAGCTTTTGAGGAACATTTCTGGAGGAGATAGGGCAGCCGGTACTGGTGCAGTTAAGAAGACAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

661

Amino Acids

76.77

Weight (kDa)

8.75

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 188 - 626 1.3e-100 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 713, 1350
Acc36I ACCTGC 1 cut(s) 1861
AccI GTMKAC 1 cut(s) 617
AciI CCGC 2 cut(s) 71, 851
AclI AACGTT 1 cut(s) 6
AclWI GGATC 1 cut(s) 1399
AcsI RAATTY 3 cut(s) 110, 844, 1355
AfaI GTAC 7 cut(s) 580, 1343, 1365, 1439, 1863, 1901, 1957
AfiI CCNNNNNNNGG 1 cut(s) 750
AflII CTTAAG 1 cut(s) 434
AflIII ACRYGT 2 cut(s) 1801, 1902
AgsI TTSAA 9 cut(s) 115, 260, 409, 866, 917, 1339, 1444, 1481, 1615
AjnI CCWGG 3 cut(s) 145, 813, 1771
AloI GAACNNNNNNTCC 2 cut(s) 1791, 1823
Alw21I GWGCWC 2 cut(s) 1552, 1910
Alw26I GTCTC 1 cut(s) 182
Alw44I GTGCAC 1 cut(s) 1548
AlwI GGATC 1 cut(s) 1399
AlwNI CAGNNNCTG 1 cut(s) 691
Ama87I CYCGRG 2 cut(s) 1103, 1844
AoxI GGCC 4 cut(s) 729, 1637, 1774, 1893
ApaLI GTGCAC 1 cut(s) 1548
ApeKI GCWGC 9 cut(s) 176, 203, 697, 826, 1099, 1574, 1618, 1781, 1949
ApoI RAATTY 3 cut(s) 110, 844, 1355
AseI ATTAAT 1 cut(s) 1131
Asp700I GAANNNNTTC 3 cut(s) 404, 912, 1803
AspLEI GCGC 3 cut(s) 181, 1284, 1724
AspS9I GGNCC 1 cut(s) 729
AvaI CYCGRG 2 cut(s) 1103, 1844
BaeGI GKGCMC 2 cut(s) 836, 1552
BauI CACGAG 1 cut(s) 1647
BbsI GAAGAC 2 cut(s) 1216, 1979
Bbv12I GWGCWC 2 cut(s) 1552, 1910
BbvI GCAGC 9 cut(s) 188, 190, 684, 838, 1111, 1586, 1605, 1768, 1961
BccI CCATC 6 cut(s) 238, 290, 389, 599, 777, 1478
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 1835, 1869
BciT130I CCWGG 3 cut(s) 147, 815, 1773
BclI TGATCA 3 cut(s) 1530, 1744, 1815
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 5 cut(s) 780, 1422, 1505, 1730, 1778
BfmI CTRYAG 3 cut(s) 204, 334, 659
BfoI RGCGCY 2 cut(s) 182, 1285
BfrI CTTAAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 1861
BglII AGATCT 1 cut(s) 1113
BmcAI AGTACT 1 cut(s) 1439
Bme1390I CCNGG 3 cut(s) 147, 815, 1773
BmeT110I CYCGRG 2 cut(s) 1103, 1844
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 2 cut(s) 51, 1851
BmrFI CCNGG 3 cut(s) 147, 815, 1773
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 3 cut(s) 265, 949, 1597
BmuI ACTGGG 1 cut(s) 739
BpiI GAAGAC 2 cut(s) 1216, 1979
BpmI CTGGAG 2 cut(s) 974, 1956
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 4 cut(s) 145, 607, 814, 1845
BsaXI ACNNNNNCTCC 4 cut(s) 1421, 1451, 1791, 1821
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse118I RCCGGY 1 cut(s) 1952
Bse1I ACTGG 6 cut(s) 533, 696, 745, 991, 1411, 1963
Bse3DI GCAATG 3 cut(s) 328, 680, 1514
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 3 cut(s) 147, 815, 1773
BseDI CCNNGG 4 cut(s) 145, 607, 814, 1845
BseGI GGATG 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 3 cut(s) 328, 680, 1514
BseMII CTCAG 2 cut(s) 377, 1287
BseNI ACTGG 6 cut(s) 533, 696, 745, 991, 1411, 1963
BseRI GAGGAG 2 cut(s) 29, 1953
BseSI GKGCMC 2 cut(s) 836, 1552
BseXI GCAGC 9 cut(s) 188, 190, 684, 838, 1111, 1586, 1605, 1768, 1961
BseYI CCCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 1983
BshFI GGCC 4 cut(s) 731, 1639, 1776, 1895
BsiHKAI GWGCWC 2 cut(s) 1552, 1910
BsiHKCI CYCGRG 2 cut(s) 1103, 1844
BsiSI CCGG 1 cut(s) 1953
BslFI GGGAC 3 cut(s) 128, 278, 548
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 128, 278, 548
BsnI GGCC 4 cut(s) 731, 1639, 1776, 1895
BsoBI CYCGRG 2 cut(s) 1103, 1844
Bsp1286I GDGCHC 3 cut(s) 836, 1552, 1910
Bsp143I GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
BspACI CCGC 2 cut(s) 71, 851
BspANI GGCC 4 cut(s) 731, 1639, 1776, 1895
BspCNI CTCAG 2 cut(s) 378, 1288
BspHI TCATGA 3 cut(s) 870, 1533, 1747
BspLI GGNNCC 2 cut(s) 51, 1851
BspMAI CTGCAG 2 cut(s) 208, 663
BspMI ACCTGC 1 cut(s) 1861
BspPI GGATC 1 cut(s) 1399
BspTI CTTAAG 1 cut(s) 434
BsrDI GCAATG 3 cut(s) 328, 680, 1514
BsrFI RCCGGY 1 cut(s) 1952
BsrI ACTGG 6 cut(s) 533, 696, 745, 991, 1411, 1963
BssAI RCCGGY 1 cut(s) 1952
BssECI CCNNGG 4 cut(s) 145, 607, 814, 1845
BssMI GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
BssNAI GTATAC 1 cut(s) 618
BssSI CACGAG 1 cut(s) 1647
BssT1I CCWWGG 1 cut(s) 607
Bst1107I GTATAC 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 1647
Bst2UI CCWGG 3 cut(s) 147, 815, 1773
Bst4CI ACNGT 6 cut(s) 89, 624, 1069, 1270, 1346, 1874
Bst6I CTCTTC 2 cut(s) 15, 72
BstAFI CTTAAG 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 1719
BstC8I GCNNGC 3 cut(s) 174, 1008, 1462
BstDEI CTNAG 2 cut(s) 386, 1296
BstF5I GGATG 1 cut(s) 249
BstH2I RGCGCY 2 cut(s) 182, 1285
BstHHI GCGC 3 cut(s) 181, 1284, 1724
BstKTI GATC 5 cut(s) 1116, 1394, 1533, 1747, 1818
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
BstMWI GCNNNNNNNGC 8 cut(s) 178, 658, 776, 1574, 1627, 1636, 1719, 1892
BstNI CCWGG 3 cut(s) 147, 815, 1773
BstNSI RCATGY 2 cut(s) 1805, 1906
BstSCI CCNGG 3 cut(s) 145, 813, 1771
BstSFI CTRYAG 3 cut(s) 204, 334, 659
BstSLI GKGCMC 2 cut(s) 836, 1552
BstV1I GCAGC 9 cut(s) 188, 190, 684, 838, 1111, 1586, 1605, 1768, 1961
BstV2I GAAGAC 2 cut(s) 1216, 1979
BstX2I RGATCY 1 cut(s) 1113
BstYI RGATCY 1 cut(s) 1113
BstZ17I GTATAC 1 cut(s) 618
BsuRI GGCC 4 cut(s) 731, 1639, 1776, 1895
BtgZI GCGATG 1 cut(s) 1229
BtsCI GGATG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 526
BveI ACCTGC 1 cut(s) 1861
Cac8I GCNNGC 3 cut(s) 174, 1008, 1462
CaiI CAGNNNCTG 1 cut(s) 691
CciI TCATGA 3 cut(s) 870, 1533, 1747
CfoI GCGC 3 cut(s) 181, 1284, 1724
Cfr10I RCCGGY 1 cut(s) 1952
Cfr13I GGNCC 1 cut(s) 729
Csp6I GTAC 7 cut(s) 579, 1342, 1364, 1438, 1862, 1900, 1956
CspCI CAANNNNNGTGG 2 cut(s) 1645, 1680
CviAII CATG 9 cut(s) 482, 871, 1204, 1232, 1534, 1627, 1748, 1802, 1903
CviQI GTAC 7 cut(s) 579, 1342, 1364, 1438, 1862, 1900, 1956
DdeI CTNAG 2 cut(s) 386, 1296
DpnI GATC 5 cut(s) 1115, 1393, 1532, 1746, 1817
DpnII GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
Eam1104I CTCTTC 2 cut(s) 15, 72
EarI CTCTTC 2 cut(s) 15, 72
EciI GGCGGA 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 607
Eco88I CYCGRG 2 cut(s) 1103, 1844
EcoRI GAATTC 2 cut(s) 110, 844
EcoRII CCWGG 3 cut(s) 145, 813, 1771
EcoT14I CCWWGG 1 cut(s) 607
EcoT22I ATGCAT 2 cut(s) 1612, 1632
ErhI CCWWGG 1 cut(s) 607
FaeI CATG 9 cut(s) 485, 874, 1207, 1235, 1537, 1630, 1751, 1805, 1906
FalI AAGNNNNNCTT 4 cut(s) 1424, 1456, 1473, 1505
FaqI GGGAC 3 cut(s) 128, 278, 548
FatI CATG 9 cut(s) 481, 870, 1203, 1231, 1533, 1626, 1747, 1801, 1902
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 3 cut(s) 1530, 1744, 1815
FblI GTMKAC 1 cut(s) 617
FokI GGATG 1 cut(s) 256
FspBI CTAG 5 cut(s) 780, 1422, 1505, 1730, 1778
GlaI GCGC 3 cut(s) 180, 1283, 1723
GsaI CCCAGC 1 cut(s) 833
GsuI CTGGAG 2 cut(s) 974, 1956
HaeII RGCGCY 2 cut(s) 182, 1285
HaeIII GGCC 4 cut(s) 731, 1639, 1776, 1895
HapII CCGG 1 cut(s) 1953
HhaI GCGC 3 cut(s) 181, 1284, 1724
Hin1II CATG 9 cut(s) 485, 874, 1207, 1235, 1537, 1630, 1751, 1805, 1906
Hin6I GCGC 3 cut(s) 179, 1282, 1722
HinP1I GCGC 3 cut(s) 179, 1282, 1722
HincII GTYRAC 2 cut(s) 1062, 1512
HindII GTYRAC 2 cut(s) 1062, 1512
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 7 cut(s) 141, 446, 948, 1037, 1221, 1276, 1407
HpaII CCGG 1 cut(s) 1953
Hpy166II GTNNAC 7 cut(s) 618, 1062, 1180, 1416, 1512, 1550, 1582
Hpy188I TCNGA 3 cut(s) 953, 1042, 1297
Hpy188III TCNNGA 8 cut(s) 115, 751, 871, 1505, 1534, 1748, 1844, 1935
Hpy8I GTNNAC 7 cut(s) 618, 1062, 1180, 1416, 1512, 1550, 1582
HpyAV CCTTC 4 cut(s) 518, 1426, 1458, 1736
HpyCH4III ACNGT 6 cut(s) 89, 624, 1069, 1270, 1346, 1874
HpyCH4IV ACGT 2 cut(s) 6, 1381
HpyF10VI GCNNNNNNNGC 8 cut(s) 178, 658, 776, 1574, 1627, 1636, 1719, 1892
HpyF3I CTNAG 2 cut(s) 386, 1296
HpySE526I ACGT 2 cut(s) 6, 1381
Hsp92II CATG 9 cut(s) 485, 874, 1207, 1235, 1537, 1630, 1751, 1805, 1906
HspAI GCGC 3 cut(s) 179, 1282, 1722
Ksp22I TGATCA 3 cut(s) 1530, 1744, 1815
Kzo9I GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
LmnI GCTCC 1 cut(s) 1474
Lsp1109I GCAGC 9 cut(s) 188, 190, 684, 838, 1111, 1586, 1605, 1768, 1961
LweI GCATC 3 cut(s) 265, 949, 1597
MaeI CTAG 5 cut(s) 780, 1422, 1505, 1730, 1778
MaeII ACGT 2 cut(s) 6, 1381
MaeIII GTNAC 5 cut(s) 379, 447, 687, 993, 1699
MalI GATC 5 cut(s) 1115, 1393, 1532, 1746, 1817
MboI GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
MboII GAAGA 9 cut(s) 32, 86, 89, 511, 886, 1216, 1387, 1493, 1891
MfeI CAATTG 2 cut(s) 409, 1662
MflI RGATCY 1 cut(s) 1113
MhlI GDGCHC 3 cut(s) 836, 1552, 1910
MlyI GAGTC 4 cut(s) 150, 455, 1046, 1230
Mph1103I ATGCAT 2 cut(s) 1612, 1632
MroXI GAANNNNTTC 3 cut(s) 404, 912, 1803
MseI TTAA 4 cut(s) 66, 435, 1131, 1968
MslI CAYNNNNRTG 2 cut(s) 1469, 1708
MspA1I CMGCKG 4 cut(s) 312, 377, 829, 1577
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 1953
MspR9I CCNGG 3 cut(s) 147, 815, 1773
MunI CAATTG 2 cut(s) 409, 1662
MvaI CCWGG 3 cut(s) 147, 815, 1773
MwoI GCNNNNNNNGC 8 cut(s) 178, 658, 776, 1574, 1627, 1636, 1719, 1892
NdeI CATATG 1 cut(s) 1012
NdeII GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
NlaIII CATG 9 cut(s) 485, 874, 1207, 1235, 1537, 1630, 1751, 1805, 1906
NlaIV GGNNCC 2 cut(s) 51, 1851
NmeAIII GCCGAG 1 cut(s) 1921
NmuCI GTSAC 1 cut(s) 447
NsiI ATGCAT 2 cut(s) 1612, 1632
NspI RCATGY 2 cut(s) 1805, 1906
PagI TCATGA 3 cut(s) 870, 1533, 1747
PciI ACATGT 2 cut(s) 1801, 1902
PdmI GAANNNNTTC 3 cut(s) 404, 912, 1803
PfeI GAWTC 3 cut(s) 948, 1276, 1407
PleI GAGTC 4 cut(s) 149, 454, 1045, 1229
PpsI GAGTC 4 cut(s) 149, 454, 1045, 1229
PscI ACATGT 2 cut(s) 1801, 1902
PshBI ATTAAT 1 cut(s) 1131
PsiI TTATAA 2 cut(s) 713, 1350
Psp1406I AACGTT 1 cut(s) 6
Psp6I CCWGG 3 cut(s) 145, 813, 1771
PspFI CCCAGC 1 cut(s) 829
PspGI CCWGG 3 cut(s) 145, 813, 1771
PspN4I GGNNCC 2 cut(s) 51, 1851
PspPI GGNCC 1 cut(s) 729
PstI CTGCAG 2 cut(s) 208, 663
PstNI CAGNNNCTG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 1113
PvuII CAGCTG 4 cut(s) 312, 377, 829, 1577
RsaI GTAC 7 cut(s) 580, 1343, 1365, 1439, 1863, 1901, 1957
RsaNI GTAC 7 cut(s) 579, 1342, 1364, 1438, 1862, 1900, 1956
RseI CAYNNNNRTG 2 cut(s) 1469, 1708
SaqAI TTAA 4 cut(s) 66, 435, 1131, 1968
Sau3AI GATC 5 cut(s) 1113, 1391, 1530, 1744, 1815
Sau96I GGNCC 1 cut(s) 729
ScaI AGTACT 1 cut(s) 1439
SchI GAGTC 4 cut(s) 150, 455, 1046, 1230
ScrFI CCNGG 3 cut(s) 147, 815, 1773
SduI GDGCHC 3 cut(s) 836, 1552, 1910
SfaNI GCATC 3 cut(s) 265, 949, 1597
SfcI CTRYAG 3 cut(s) 204, 334, 659
SmiMI CAYNNNNRTG 2 cut(s) 1469, 1708
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SsiI CCGC 2 cut(s) 71, 851
SspI AATATT 1 cut(s) 428
SspMI CTAG 5 cut(s) 780, 1422, 1505, 1730, 1778
StyD4I CCNGG 3 cut(s) 145, 813, 1771
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 6 cut(s) 89, 624, 1069, 1270, 1346, 1874
TaiI ACGT 2 cut(s) 9, 1384
TaqI TCGA 3 cut(s) 210, 235, 1494
TatI WGTACW 3 cut(s) 1341, 1437, 1899
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 3 cut(s) 948, 1276, 1407
Tru1I TTAA 4 cut(s) 66, 435, 1131, 1968
Tru9I TTAA 4 cut(s) 66, 435, 1131, 1968
TscAI CASTG 1 cut(s) 533
TseFI GTSAC 1 cut(s) 447
TseI GCWGC 9 cut(s) 176, 203, 697, 826, 1099, 1574, 1618, 1781, 1949
Tsp45I GTSAC 1 cut(s) 447
TspRI CASTG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 434
VneI GTGCAC 1 cut(s) 1548
VspI ATTAAT 1 cut(s) 1131
XapI RAATTY 3 cut(s) 110, 844, 1355
XbaI TCTAGA 1 cut(s) 1504
XceI RCATGY 2 cut(s) 1805, 1906
XmiI GTMKAC 1 cut(s) 617
XmnI GAANNNNTTC 3 cut(s) 404, 912, 1803
XspI CTAG 5 cut(s) 780, 1422, 1505, 1730, 1778
ZrmI AGTACT 1 cut(s) 1439
Zsp2I ATGCAT 2 cut(s) 1612, 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.