Rh2AG423600

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006221
Physical Location & Seq
Forward (+)
62930205 .. 62936920
6716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG423600.1

Sequence Viewer

Length: 1923 bp
ATGAATGTTGTTGAGGAGGAACAAATTGCTTTTGAGATTGGGGTTGTGGTTCCAAAGAGAAATGTCAAGGCAGAAGAAGAAGATGACTGTGTGGACGTTTTGGTTAAGAAATTTAAGAATGTGGGTTTGGTAGTTGAGAGAGTCCCTGGCGTTGTCGATGACTTTATCAAGCTGGCAGCGCCTTTAGAGACATTGGGAAGGGCTGCGGTTGAGTTGCAAATTAAAAAGCGAACTCAAATTGGGATGGATTTACAATTTGAATGGGAGGAGGTTAATGCTTTTGTGAGGCAGCCTGATGGTTCATTGTTCAGCTGGTGTGAGCGTTTTCATTGCTATAGGCACTTGATATATGGAATTATGAACAAGAGCAAGTCAGCTATAACTCTGAGATTTGATGGGAAAGAGTTCTATTGGGAAGTTGGGGAATGTTTGCTTAAGAAATTGGAGTCACAGAGGATTGTCAAAGAAGTTTTTCCTTTGCATGATGAAGTGAAAAGGAAGAAACTCCTTCACACTTGGGCTCTGCACTGGTGGGACTTCACAAGCCAGCCCATAGATGAGATTTACTCTTATTTTGGTACAAAGATTGCCATCTATTTTGCTTTTCTTGGAATGTATACACGGTGGTTGCTGTTTCCAGCTACATTTGGACTTATTCTGCAGATATTTGATTTTGGGTCGTTGCAGTTACTGGTGCTCCCCCTTTTCTTTATAAGCATAACCTTATGGGCTATAATGTTTTCCCAGTTCTGGAAACGCAAAAATGTTGCTCTGTTAACTAGATGGCAGATCAGTTATCCAATTAGAACCGACCTGGGAAATAGGCAGCTGGGCATGGCTTGGAGTTCCTCGCAGTCATCTGTAGAACTCATGAAGAAATGGGGGACAGACAAAACAAAAGAGAAAGAAATGTTTCAAAGAACTGAGTGGTTTGGACGACTACTGAGATTCCGAAATGATGCTATCATTATAGTTAGTATTATATGCCTCCAGTTACCGTTTGAGCTGGCATATGCTCATCTTTACGAGGTTATTAAGTCTGATATTGTGAAGTTTGGGTTGACAACCATATATCTTTTTGCAATTCAGTTTTTTACGAAGCTAGGGGGCAAGATCTCTGTTAAGCTTATTAAACACGAAGATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATACATAGGAGTCTTCTACCATGCCCTTATACACCGGAACTTTTTGACTCTTCGTACAGTCTTGATTCAGCGCCTCCTTGTGTCTGAGGTATTGGAAAACTTGGTGGAAAACACTTTACCATATTTGAAGTACAGTTATAAGAAGTTCAGAGTTCGAAGCAAGAAGAAACGTGAAAGAGGATCATCAACAGGGAAGATCCACTTTGCTTCTAGGGTGGAAAAGGAGTACTTGAAGCCTTCTTATTCTGCAAGCATCGGTGTGGAACTTGAAGATGGGCTTTTCGATGACTTTCTAGAGTTAGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCCTTCCCCCCTGCTTTTGCCTTTGCGGCTGTGAACAACATAACTGAAATTAGGACAGATGCATTGAAGCTGCTTGTGATGCATAAGAGGCCAATTCCTCGTGTTTCCACGACAATTGGTGCTTGGCTGAACATATTTCAGTTTCTTGTTGTTACATCCATATGCACCAACTGCGCCCTTCTAGTATGGTTATATGATCATGAGGGGAAATGGAATATAGAGCCTGGCCTAGCTGCAATTCTGGTTATGGAACATATCCTCCTGTTGATTAAGTTTGGTTTCTCTCGCCTTGTACCCGAGGTGATATTAAATCACTCCTGTGAACACTTGCTTACATCTCTTACTGTATTTAGAGTATGTAATTGTTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

640

Amino Acids

74.6

Weight (kDa)

8.96

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 188 - 617 1.2e-96 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 713, 1350
AasI GACNNNNNNGTC 1 cut(s) 1175
AccI GTMKAC 2 cut(s) 617, 1179
AciI CCGC 2 cut(s) 206, 1574
AclWI GGATC 2 cut(s) 1399, 1402
AcsI RAATTY 1 cut(s) 110
AfaI GTAC 5 cut(s) 580, 1267, 1343, 1439, 1842
AfiI CCNNNNNNNGG 1 cut(s) 750
AflII CTTAAG 1 cut(s) 434
AgsI TTSAA 6 cut(s) 260, 917, 1339, 1444, 1481, 1615
AjnI CCWGG 3 cut(s) 145, 813, 1771
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AleI CACNNNNGTG 1 cut(s) 1866
AloI GAACNNNNNNTCC 2 cut(s) 1791, 1823
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 2 cut(s) 1399, 1402
AlwNI CAGNNNCTG 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 1844
AoxI GGCC 2 cut(s) 1637, 1774
ApeKI GCWGC 6 cut(s) 176, 203, 289, 826, 1618, 1781
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 3 cut(s) 404, 471, 912
AspLEI GCGC 3 cut(s) 181, 1284, 1724
AsuHPI GGTGA 1 cut(s) 1861
AsuII TTCGAA 1 cut(s) 1366
AvaI CYCGRG 1 cut(s) 1844
BanII GRGCYC 1 cut(s) 523
BauI CACGAG 1 cut(s) 1647
BbsI GAAGAC 1 cut(s) 1216
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 6 cut(s) 188, 190, 301, 838, 1605, 1768
BccI CCATC 6 cut(s) 238, 290, 389, 599, 777, 1478
BciT130I CCWGG 3 cut(s) 147, 815, 1773
BclI TGATCA 2 cut(s) 1530, 1744
BcoDI GTCTC 1 cut(s) 182
BfaI CTAG 6 cut(s) 780, 1103, 1422, 1505, 1730, 1778
BfmI CTRYAG 3 cut(s) 334, 659, 861
BfoI RGCGCY 2 cut(s) 182, 1285
BfrI CTTAAG 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 1574
BglII AGATCT 1 cut(s) 1113
BisI GCNGC 7 cut(s) 177, 204, 290, 827, 1575, 1619, 1782
BlsI GCNGC 7 cut(s) 178, 205, 291, 828, 1576, 1620, 1783
BmcAI AGTACT 1 cut(s) 1439
Bme1390I CCNGG 3 cut(s) 147, 815, 1773
BmeT110I CYCGRG 1 cut(s) 1844
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 3 cut(s) 147, 815, 1773
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 4 cut(s) 949, 1473, 1597, 1617
BmuI ACTGGG 1 cut(s) 739
BpiI GAAGAC 1 cut(s) 1216
BplI GAGNNNNNCTC 2 cut(s) 551, 583
BpmI CTGGAG 1 cut(s) 974
Bpu14I TTCGAA 1 cut(s) 1366
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 3 cut(s) 145, 814, 1845
BsaWI WCCGGW 1 cut(s) 1245
BsaXI ACNNNNNCTCC 4 cut(s) 681, 711, 1791, 1821
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 4 cut(s) 533, 696, 745, 991
Bse3DI GCAATG 2 cut(s) 328, 1514
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 3 cut(s) 147, 815, 1773
BseDI CCNNGG 3 cut(s) 145, 814, 1845
BseGI GGATG 2 cut(s) 249, 1703
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 2 cut(s) 328, 1514
BseMII CTCAG 4 cut(s) 377, 915, 935, 1287
BseNI ACTGG 4 cut(s) 533, 696, 745, 991
BseRI GAGGAG 2 cut(s) 29, 281
BseXI GCAGC 6 cut(s) 188, 190, 301, 838, 1605, 1768
BseYI CCCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 2 cut(s) 1639, 1776
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 1844
BsiSI CCGG 1 cut(s) 1246
BslFI GGGAC 3 cut(s) 128, 548, 898
BslI CCNNNNNNNGG 1 cut(s) 750
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 128, 548, 898
BsnI GGCC 2 cut(s) 1639, 1776
BsoBI CYCGRG 1 cut(s) 1844
Bsp119I TTCGAA 1 cut(s) 1366
Bsp1286I GDGCHC 2 cut(s) 523, 699
Bsp143I GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
BspACI CCGC 2 cut(s) 206, 1574
BspANI GGCC 2 cut(s) 1639, 1776
BspCNI CTCAG 4 cut(s) 378, 916, 936, 1288
BspHI TCATGA 3 cut(s) 870, 1533, 1747
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 663
BspPI GGATC 2 cut(s) 1399, 1402
BspT104I TTCGAA 1 cut(s) 1366
BspTI CTTAAG 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 328, 1514
BsrI ACTGG 4 cut(s) 533, 696, 745, 991
BssECI CCNNGG 3 cut(s) 145, 814, 1845
BssMI GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
BssNAI GTATAC 1 cut(s) 618
BssSI CACGAG 1 cut(s) 1647
Bst1107I GTATAC 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 1647
Bst2UI CCWGG 3 cut(s) 147, 815, 1773
Bst4CI ACNGT 6 cut(s) 89, 624, 999, 1270, 1346, 1894
Bst6I CTCTTC 1 cut(s) 1266
BstAFI CTTAAG 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 1719
BstBI TTCGAA 1 cut(s) 1366
BstC8I GCNNGC 4 cut(s) 174, 548, 1008, 1462
BstDEI CTNAG 4 cut(s) 386, 924, 944, 1296
BstF5I GGATG 2 cut(s) 249, 1703
BstH2I RGCGCY 2 cut(s) 182, 1285
BstHHI GCGC 3 cut(s) 181, 1284, 1724
BstKTI GATC 6 cut(s) 792, 1116, 1394, 1410, 1533, 1747
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
BstMWI GCNNNNNNNGC 5 cut(s) 178, 1574, 1627, 1636, 1719
BstNI CCWGG 3 cut(s) 147, 815, 1773
BstSCI CCNGG 3 cut(s) 145, 813, 1771
BstSFI CTRYAG 3 cut(s) 334, 659, 861
BstV1I GCAGC 6 cut(s) 188, 190, 301, 838, 1605, 1768
BstV2I GAAGAC 1 cut(s) 1216
BstX2I RGATCY 2 cut(s) 1113, 1407
BstYI RGATCY 2 cut(s) 1113, 1407
BstZ17I GTATAC 1 cut(s) 618
BsuRI GGCC 2 cut(s) 1639, 1776
BtsCI GGATG 2 cut(s) 249, 1703
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 4 cut(s) 174, 548, 1008, 1462
CaiI CAGNNNCTG 1 cut(s) 691
CciI TCATGA 3 cut(s) 870, 1533, 1747
CfoI GCGC 3 cut(s) 181, 1284, 1724
Csp6I GTAC 5 cut(s) 579, 1266, 1342, 1438, 1841
CspCI CAANNNNNGTGG 2 cut(s) 1645, 1680
CviAII CATG 7 cut(s) 482, 835, 871, 1204, 1232, 1534, 1748
CviQI GTAC 5 cut(s) 579, 1266, 1342, 1438, 1841
DdeI CTNAG 4 cut(s) 386, 924, 944, 1296
DpnI GATC 6 cut(s) 791, 1115, 1393, 1409, 1532, 1746
DpnII GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
Eam1104I CTCTTC 1 cut(s) 1266
EarI CTCTTC 1 cut(s) 1266
Eco24I GRGCYC 1 cut(s) 523
Eco88I CYCGRG 1 cut(s) 1844
EcoRII CCWGG 3 cut(s) 145, 813, 1771
EcoT22I ATGCAT 2 cut(s) 1612, 1632
EcoT38I GRGCYC 1 cut(s) 523
FaeI CATG 7 cut(s) 485, 838, 874, 1207, 1235, 1537, 1751
FalI AAGNNNNNCTT 6 cut(s) 1397, 1429, 1424, 1456, 1473, 1505
FaqI GGGAC 3 cut(s) 128, 548, 898
FatI CATG 7 cut(s) 481, 834, 870, 1203, 1231, 1533, 1747
FauNDI CATATG 2 cut(s) 1012, 1709
FbaI TGATCA 2 cut(s) 1530, 1744
FblI GTMKAC 2 cut(s) 617, 1179
Fnu4HI GCNGC 7 cut(s) 177, 204, 290, 827, 1575, 1619, 1782
FokI GGATG 2 cut(s) 256, 1690
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 7 cut(s) 177, 204, 290, 827, 1575, 1619, 1782
FspBI CTAG 6 cut(s) 780, 1103, 1422, 1505, 1730, 1778
GlaI GCGC 3 cut(s) 180, 1283, 1723
GluI GCNGC 7 cut(s) 177, 204, 290, 827, 1575, 1619, 1782
GsaI CCCAGC 1 cut(s) 833
GsuI CTGGAG 1 cut(s) 974
HaeII RGCGCY 2 cut(s) 182, 1285
HaeIII GGCC 2 cut(s) 1639, 1776
HapII CCGG 1 cut(s) 1246
HhaI GCGC 3 cut(s) 181, 1284, 1724
Hin1II CATG 7 cut(s) 485, 838, 874, 1207, 1235, 1537, 1751
Hin6I GCGC 3 cut(s) 179, 1282, 1722
HinP1I GCGC 3 cut(s) 179, 1282, 1722
HincII GTYRAC 2 cut(s) 777, 1062
HindII GTYRAC 2 cut(s) 777, 1062
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 6 cut(s) 141, 446, 948, 1221, 1258, 1276
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 1 cut(s) 1246
HphI GGTGA 1 cut(s) 1861
Hpy166II GTNNAC 7 cut(s) 94, 618, 777, 1062, 1180, 1582, 1871
Hpy188I TCNGA 5 cut(s) 387, 953, 1042, 1297, 1361
Hpy188III TCNNGA 6 cut(s) 751, 871, 1273, 1505, 1534, 1748
Hpy8I GTNNAC 7 cut(s) 94, 618, 777, 1062, 1180, 1582, 1871
HpyAV CCTTC 5 cut(s) 192, 518, 1458, 1561, 1736
HpyCH4III ACNGT 6 cut(s) 89, 624, 999, 1270, 1346, 1894
HpyCH4IV ACGT 2 cut(s) 96, 1381
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 1574, 1627, 1636, 1719
HpyF3I CTNAG 4 cut(s) 386, 924, 944, 1296
HpySE526I ACGT 2 cut(s) 96, 1381
Hsp92II CATG 7 cut(s) 485, 838, 874, 1207, 1235, 1537, 1751
HspAI GCGC 3 cut(s) 179, 1282, 1722
Ksp22I TGATCA 2 cut(s) 1530, 1744
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
LmnI GCTCC 1 cut(s) 702
Lsp1109I GCAGC 6 cut(s) 188, 190, 301, 838, 1605, 1768
LweI GCATC 4 cut(s) 949, 1473, 1597, 1617
MaeI CTAG 6 cut(s) 780, 1103, 1422, 1505, 1730, 1778
MaeII ACGT 2 cut(s) 96, 1381
MaeIII GTNAC 4 cut(s) 447, 687, 993, 1699
MalI GATC 6 cut(s) 791, 1115, 1393, 1409, 1532, 1746
MboI GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
MfeI CAATTG 1 cut(s) 1662
MflI RGATCY 2 cut(s) 1113, 1407
MhlI GDGCHC 2 cut(s) 523, 699
MlyI GAGTC 4 cut(s) 150, 455, 1230, 1252
MmeI TCCRAC 1 cut(s) 1895
Mph1103I ATGCAT 2 cut(s) 1612, 1632
MroXI GAANNNNTTC 3 cut(s) 404, 471, 912
MslI CAYNNNNRTG 3 cut(s) 1469, 1708, 1866
MspA1I CMGCKG 2 cut(s) 312, 829
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 1246
MspR9I CCNGG 3 cut(s) 147, 815, 1773
MunI CAATTG 1 cut(s) 1662
MvaI CCWGG 3 cut(s) 147, 815, 1773
MwoI GCNNNNNNNGC 5 cut(s) 178, 1574, 1627, 1636, 1719
NdeI CATATG 2 cut(s) 1012, 1709
NdeII GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
NlaIII CATG 7 cut(s) 485, 838, 874, 1207, 1235, 1537, 1751
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 447
NsiI ATGCAT 2 cut(s) 1612, 1632
NspV TTCGAA 1 cut(s) 1366
OliI CACNNNNGTG 1 cut(s) 1866
PagI TCATGA 3 cut(s) 870, 1533, 1747
PdmI GAANNNNTTC 3 cut(s) 404, 471, 912
PfeI GAWTC 2 cut(s) 948, 1276
PkrI GCNGC 7 cut(s) 178, 205, 291, 828, 1576, 1620, 1783
PleI GAGTC 4 cut(s) 149, 454, 1229, 1252
PpsI GAGTC 4 cut(s) 149, 454, 1229, 1252
PsiI TTATAA 2 cut(s) 713, 1350
Psp6I CCWGG 3 cut(s) 145, 813, 1771
PspFI CCCAGC 1 cut(s) 829
PspGI CCWGG 3 cut(s) 145, 813, 1771
PspN4I GGNNCC 1 cut(s) 51
PstI CTGCAG 1 cut(s) 663
PstNI CAGNNNCTG 1 cut(s) 691
PsuI RGATCY 2 cut(s) 1113, 1407
PvuII CAGCTG 2 cut(s) 312, 829
RsaI GTAC 5 cut(s) 580, 1267, 1343, 1439, 1842
RsaNI GTAC 5 cut(s) 579, 1266, 1342, 1438, 1841
RseI CAYNNNNRTG 3 cut(s) 1469, 1708, 1866
SatI GCNGC 7 cut(s) 177, 204, 290, 827, 1575, 1619, 1782
Sau3AI GATC 6 cut(s) 789, 1113, 1391, 1407, 1530, 1744
ScaI AGTACT 1 cut(s) 1439
SchI GAGTC 4 cut(s) 150, 455, 1230, 1252
ScrFI CCNGG 3 cut(s) 147, 815, 1773
SduI GDGCHC 2 cut(s) 523, 699
SfaNI GCATC 4 cut(s) 949, 1473, 1597, 1617
SfcI CTRYAG 3 cut(s) 334, 659, 861
SfuI TTCGAA 1 cut(s) 1366
SmiMI CAYNNNNRTG 3 cut(s) 1469, 1708, 1866
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
SsiI CCGC 2 cut(s) 206, 1574
SspMI CTAG 6 cut(s) 780, 1103, 1422, 1505, 1730, 1778
StyD4I CCNGG 3 cut(s) 145, 813, 1771
TaaI ACNGT 6 cut(s) 89, 624, 999, 1270, 1346, 1894
TaiI ACGT 2 cut(s) 99, 1384
TaqI TCGA 3 cut(s) 156, 1366, 1494
TatI WGTACW 2 cut(s) 1341, 1437
TauI GCSGC 1 cut(s) 1577
TfiI GAWTC 2 cut(s) 948, 1276
TscAI CASTG 1 cut(s) 533
TseFI GTSAC 1 cut(s) 447
TseI GCWGC 6 cut(s) 176, 203, 289, 826, 1618, 1781
Tsp45I GTSAC 1 cut(s) 447
TspDTI ATGAA 8 cut(s) 17, 291, 317, 374, 501, 887, 1161, 1192
TspRI CASTG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 434
XapI RAATTY 1 cut(s) 110
XbaI TCTAGA 1 cut(s) 1504
XmiI GTMKAC 2 cut(s) 617, 1179
XmnI GAANNNNTTC 3 cut(s) 404, 471, 912
XspI CTAG 6 cut(s) 780, 1103, 1422, 1505, 1730, 1778
ZrmI AGTACT 1 cut(s) 1439
Zsp2I ATGCAT 2 cut(s) 1612, 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.