Rw0G014830

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00663
OG0006221
Physical Location & Seq
Reverse (-)
59 .. 4366
4308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014830.1

Sequence Viewer

Length: 1581 bp
ATGAATGTTGTTGAGGAGAAACAAATTGCTTTTGAGATTGGGGTTGTGGTTCCAAAGAGAAATGTCAAGGCAAAAGAAGAAGATGACTGTGTGGAGGTTTTGGTTGATGAATTTAAGAATGTGGGTTTGGAAGTTGAGAGAGTCCCTGGCGTTGTCGATGACTTTATCAAGCTGGCAGCGCCTTTAGAGATATTGGGGAAGGCTGCGGTTGAGTTGCAAATTAAAAAGCGAACTCAAATTGGGATGGATTTACAGTTTGAATGGGAGGAGGTTAATGCTTTTGTGAGGCAGCCTGATGGTTCATTGTTCAGCTGGTGTGAGCGTTTTCATTGCTATAGGCACTTGATATATGGAATTATGAACAAGAGCAAGTCAGCTAGAACTCTGAGATTTGATGGGAAAGAGTTCTATTGGGAAGTTCGGGAATGTTTGCTTAAGAAATTGGAGTCACAGAGGATTGTCAAAGAAGTTTTTCCTTTGCATGATGAAGTGAAAAGGAAGAAACTCCTTCACTCTTGGGCTCTGCACTGGTGGGACTTCACAAGCCAGCCCATAGATGAGATTTACTCATATTTTGGTACAAAGATTGCCATCTATTTTGCTTTTCTTGGAATGTATACACGGTGGTTGCTGTTTCCAGCTACATTTGGACTTATTCTGCAGATATTTGATTTTGGGTCGTTGCAGTTACTGGTGCTCCCCCTTTTCTTTATAAGCATAACCTTATGGGCTATAATGTTTTCCCAGTTCTGGAAACGCAAAAATGTTGCTCTGTTAACTAGATGGCAGATCAGTTATCCAATTAGAACCGACCTGGGAAATAGGCAGCTGGGCATGGCTTGGAGTTCCTCGCAGTCGTCTGTAGAACTCATGAAGAAATGGGGGACAGACAAAACAAAAGAGAAAGAAATGTTTCAAAGAATTGAGTGGTTTGGACGACTACTGAGATTCCGAAATGATGCTATCATTATAGTTAGTATTATATGCCTTCAGTTGCCGTTTGAGCTGGCATATGCTCATCTGTACGAGGTTATTAAGTCTGATATTGTGAAGTTTGGGTTGACAACCATATATCTTTTTGCAATTCAGTTTTTTACGAAGCTAGGGGGCAAGATCTCTGTTAAGCTTATTAAACACGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATACATAGGAGTCTTCTACCATGCCCTTATACACCGGAACTTTTTGACTCTTCGTACAGTCTTGATTCAGCGCCTCCTTGTGTCTGAGGTATTGGAAAACTTGGTGGAAAACACTTTACCATATTTGAAGTACAGTTATAAGAAGTTCAGAGTTCGAAGCAAGAAGAAACGCGAAAGAGGATCATCAACAGGGAGGATCCACTTTGCTTCTAGGGTGGAAAAGGAGTACTTGAAGCCTTCTTATTCTGCAAGCATTGGTGTGGAACTTGAAGATGGGCTTTTCGATGACTTTCTAGAGTTAGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCCTTCCCCCCTGCTTTTGCCTTTGCGGCTGTG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

527

Amino Acids

61.83

Weight (kDa)

9.14

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 188 - 526 1.9e-64 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 713, 1350
AasI GACNNNNNNGTC 1 cut(s) 1175
AccI GTMKAC 2 cut(s) 617, 1179
AccII CGCG 1 cut(s) 1383
AciI CCGC 2 cut(s) 206, 1574
AclWI GGATC 3 cut(s) 1399, 1402, 1415
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 974
AfaI GTAC 5 cut(s) 580, 1025, 1267, 1343, 1439
AfiI CCNNNNNNNGG 1 cut(s) 750
AflII CTTAAG 1 cut(s) 434
AgsI TTSAA 5 cut(s) 260, 917, 1339, 1444, 1481
AjnI CCWGG 2 cut(s) 145, 813
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
Alw21I GWGCWC 1 cut(s) 699
AlwI GGATC 3 cut(s) 1399, 1402, 1415
AlwNI CAGNNNCTG 1 cut(s) 691
ApeKI GCWGC 4 cut(s) 176, 203, 289, 826
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 3 cut(s) 404, 471, 912
AspLEI GCGC 2 cut(s) 181, 1284
AsuII TTCGAA 1 cut(s) 1366
BamHI GGATCC 1 cut(s) 1407
BanII GRGCYC 1 cut(s) 523
BbsI GAAGAC 1 cut(s) 1216
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 4 cut(s) 188, 190, 301, 838
BccI CCATC 6 cut(s) 238, 290, 389, 599, 777, 1478
BceAI ACGGC 1 cut(s) 982
BciT130I CCWGG 2 cut(s) 147, 815
BclI TGATCA 1 cut(s) 1530
BfaI CTAG 5 cut(s) 378, 780, 1103, 1422, 1505
BfmI CTRYAG 3 cut(s) 334, 659, 861
BfoI RGCGCY 2 cut(s) 182, 1285
BfrI CTTAAG 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 1574
BglII AGATCT 1 cut(s) 1113
BisI GCNGC 5 cut(s) 177, 204, 290, 827, 1575
BlsI GCNGC 5 cut(s) 178, 205, 291, 828, 1576
BmcAI AGTACT 1 cut(s) 1439
Bme1390I CCNGG 2 cut(s) 147, 815
BmiI GGNNCC 2 cut(s) 51, 1409
BmrFI CCNGG 2 cut(s) 147, 815
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 1 cut(s) 949
BmuI ACTGGG 1 cut(s) 739
BpiI GAAGAC 1 cut(s) 1216
BplI GAGNNNNNCTC 2 cut(s) 551, 583
Bpu14I TTCGAA 1 cut(s) 1366
BsaBI GATNNNNATC 1 cut(s) 590
BsaJI CCNNGG 2 cut(s) 145, 814
BsaWI WCCGGW 1 cut(s) 1245
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 1 cut(s) 750
Bse1I ACTGG 3 cut(s) 533, 696, 745
Bse3DI GCAATG 2 cut(s) 328, 1514
Bse8I GATNNNNATC 1 cut(s) 590
BseBI CCWGG 2 cut(s) 147, 815
BseDI CCNNGG 2 cut(s) 145, 814
BseGI GGATG 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 590
BseLI CCNNNNNNNGG 1 cut(s) 750
BseMI GCAATG 2 cut(s) 328, 1514
BseMII CTCAG 3 cut(s) 377, 935, 1287
BseNI ACTGG 3 cut(s) 533, 696, 745
BseRI GAGGAG 2 cut(s) 29, 281
BseXI GCAGC 4 cut(s) 188, 190, 301, 838
BseYI CCCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 1383
BsiHKAI GWGCWC 1 cut(s) 699
BsiSI CCGG 1 cut(s) 1246
BslFI GGGAC 3 cut(s) 128, 548, 898
BslI CCNNNNNNNGG 1 cut(s) 750
BsmFI GGGAC 3 cut(s) 128, 548, 898
Bsp119I TTCGAA 1 cut(s) 1366
Bsp1286I GDGCHC 2 cut(s) 523, 699
Bsp143I GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
BspACI CCGC 2 cut(s) 206, 1574
BspCNI CTCAG 3 cut(s) 378, 936, 1288
BspFNI CGCG 1 cut(s) 1383
BspHI TCATGA 2 cut(s) 870, 1533
BspLI GGNNCC 2 cut(s) 51, 1409
BspMAI CTGCAG 1 cut(s) 663
BspPI GGATC 3 cut(s) 1399, 1402, 1415
BspT104I TTCGAA 1 cut(s) 1366
BspTI CTTAAG 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 328, 1514
BsrI ACTGG 3 cut(s) 533, 696, 745
BssECI CCNNGG 2 cut(s) 145, 814
BssMI GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
BssNAI GTATAC 1 cut(s) 618
Bst1107I GTATAC 1 cut(s) 618
Bst2UI CCWGG 2 cut(s) 147, 815
Bst4CI ACNGT 5 cut(s) 89, 255, 624, 1270, 1346
Bst6I CTCTTC 1 cut(s) 1266
BstAFI CTTAAG 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 1366
BstC8I GCNNGC 4 cut(s) 174, 548, 1008, 1462
BstDEI CTNAG 3 cut(s) 386, 944, 1296
BstF5I GGATG 1 cut(s) 249
BstFNI CGCG 1 cut(s) 1383
BstH2I RGCGCY 2 cut(s) 182, 1285
BstHHI GCGC 2 cut(s) 181, 1284
BstKTI GATC 5 cut(s) 792, 1116, 1394, 1410, 1533
BstMBI GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
BstMWI GCNNNNNNNGC 3 cut(s) 178, 1003, 1574
BstNI CCWGG 2 cut(s) 147, 815
BstSCI CCNGG 2 cut(s) 145, 813
BstSFI CTRYAG 3 cut(s) 334, 659, 861
BstUI CGCG 1 cut(s) 1383
BstV1I GCAGC 4 cut(s) 188, 190, 301, 838
BstV2I GAAGAC 1 cut(s) 1216
BstX2I RGATCY 2 cut(s) 1113, 1407
BstYI RGATCY 2 cut(s) 1113, 1407
BstZ17I GTATAC 1 cut(s) 618
BtsCI GGATG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 4 cut(s) 174, 548, 1008, 1462
CaiI CAGNNNCTG 1 cut(s) 691
CciI TCATGA 2 cut(s) 870, 1533
CfoI GCGC 2 cut(s) 181, 1284
Csp6I GTAC 5 cut(s) 579, 1024, 1266, 1342, 1438
CviAII CATG 6 cut(s) 482, 835, 871, 1204, 1232, 1534
CviQI GTAC 5 cut(s) 579, 1024, 1266, 1342, 1438
DdeI CTNAG 3 cut(s) 386, 944, 1296
DpnI GATC 5 cut(s) 791, 1115, 1393, 1409, 1532
DpnII GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
Eam1104I CTCTTC 1 cut(s) 1266
EarI CTCTTC 1 cut(s) 1266
Eco24I GRGCYC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 974
EcoRII CCWGG 2 cut(s) 145, 813
EcoT38I GRGCYC 1 cut(s) 523
FaeI CATG 6 cut(s) 485, 838, 874, 1207, 1235, 1537
FalI AAGNNNNNCTT 4 cut(s) 1424, 1456, 1473, 1505
FaqI GGGAC 3 cut(s) 128, 548, 898
FatI CATG 6 cut(s) 481, 834, 870, 1203, 1231, 1533
FauNDI CATATG 1 cut(s) 1012
FbaI TGATCA 1 cut(s) 1530
FblI GTMKAC 2 cut(s) 617, 1179
Fnu4HI GCNGC 5 cut(s) 177, 204, 290, 827, 1575
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 5 cut(s) 177, 204, 290, 827, 1575
FspBI CTAG 5 cut(s) 378, 780, 1103, 1422, 1505
GlaI GCGC 2 cut(s) 180, 1283
GluI GCNGC 5 cut(s) 177, 204, 290, 827, 1575
GsaI CCCAGC 1 cut(s) 833
HaeII RGCGCY 2 cut(s) 182, 1285
HapII CCGG 1 cut(s) 1246
HhaI GCGC 2 cut(s) 181, 1284
Hin1II CATG 6 cut(s) 485, 838, 874, 1207, 1235, 1537
Hin6I GCGC 2 cut(s) 179, 1282
HinP1I GCGC 2 cut(s) 179, 1282
HincII GTYRAC 2 cut(s) 777, 1062
HindII GTYRAC 2 cut(s) 777, 1062
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 6 cut(s) 141, 446, 948, 1221, 1258, 1276
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 1 cut(s) 1246
Hpy166II GTNNAC 4 cut(s) 618, 777, 1062, 1180
Hpy188I TCNGA 5 cut(s) 387, 953, 1042, 1297, 1361
Hpy188III TCNNGA 6 cut(s) 422, 751, 871, 1273, 1505, 1534
Hpy8I GTNNAC 4 cut(s) 618, 777, 1062, 1180
HpyAV CCTTC 5 cut(s) 193, 518, 998, 1458, 1561
HpyCH4III ACNGT 5 cut(s) 89, 255, 624, 1270, 1346
HpyCH4V TGCA 9 cut(s) 217, 481, 526, 661, 685, 1082, 1207, 1460, 1519
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 1003, 1574
HpyF3I CTNAG 3 cut(s) 386, 944, 1296
Hsp92II CATG 6 cut(s) 485, 838, 874, 1207, 1235, 1537
HspAI GCGC 2 cut(s) 179, 1282
Ksp22I TGATCA 1 cut(s) 1530
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
LmnI GCTCC 1 cut(s) 702
Lsp1109I GCAGC 4 cut(s) 188, 190, 301, 838
LweI GCATC 1 cut(s) 949
MaeI CTAG 5 cut(s) 378, 780, 1103, 1422, 1505
MaeIII GTNAC 2 cut(s) 447, 687
MalI GATC 5 cut(s) 791, 1115, 1393, 1409, 1532
MboI GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
MboII GAAGA 8 cut(s) 89, 92, 511, 886, 1216, 1253, 1387, 1493
MflI RGATCY 2 cut(s) 1113, 1407
MhlI GDGCHC 2 cut(s) 523, 699
MluCI AATT 9 cut(s) 24, 110, 219, 237, 354, 440, 801, 921, 1083
MlyI GAGTC 4 cut(s) 150, 455, 1230, 1252
MroXI GAANNNNTTC 3 cut(s) 404, 471, 912
MseI TTAA 8 cut(s) 114, 222, 273, 435, 776, 1035, 1122, 1131
MslI CAYNNNNRTG 1 cut(s) 1469
MspA1I CMGCKG 2 cut(s) 312, 829
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 1246
MspR9I CCNGG 2 cut(s) 147, 815
MvaI CCWGG 2 cut(s) 147, 815
MvnI CGCG 1 cut(s) 1383
MwoI GCNNNNNNNGC 3 cut(s) 178, 1003, 1574
NdeI CATATG 1 cut(s) 1012
NdeII GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
NlaIII CATG 6 cut(s) 485, 838, 874, 1207, 1235, 1537
NlaIV GGNNCC 2 cut(s) 51, 1409
NmuCI GTSAC 1 cut(s) 447
NspV TTCGAA 1 cut(s) 1366
PagI TCATGA 2 cut(s) 870, 1533
PdmI GAANNNNTTC 3 cut(s) 404, 471, 912
PfeI GAWTC 2 cut(s) 948, 1276
PkrI GCNGC 5 cut(s) 178, 205, 291, 828, 1576
PleI GAGTC 4 cut(s) 149, 454, 1229, 1252
PpsI GAGTC 4 cut(s) 149, 454, 1229, 1252
PsiI TTATAA 2 cut(s) 713, 1350
Psp6I CCWGG 2 cut(s) 145, 813
PspFI CCCAGC 1 cut(s) 829
PspGI CCWGG 2 cut(s) 145, 813
PspN4I GGNNCC 2 cut(s) 51, 1409
PstI CTGCAG 1 cut(s) 663
PstNI CAGNNNCTG 1 cut(s) 691
PsuI RGATCY 2 cut(s) 1113, 1407
PvuII CAGCTG 2 cut(s) 312, 829
RsaI GTAC 5 cut(s) 580, 1025, 1267, 1343, 1439
RsaNI GTAC 5 cut(s) 579, 1024, 1266, 1342, 1438
RseI CAYNNNNRTG 1 cut(s) 1469
SaqAI TTAA 8 cut(s) 114, 222, 273, 435, 776, 1035, 1122, 1131
SatI GCNGC 5 cut(s) 177, 204, 290, 827, 1575
Sau3AI GATC 5 cut(s) 789, 1113, 1391, 1407, 1530
ScaI AGTACT 1 cut(s) 1439
SchI GAGTC 4 cut(s) 150, 455, 1230, 1252
ScrFI CCNGG 2 cut(s) 147, 815
SduI GDGCHC 2 cut(s) 523, 699
SfaNI GCATC 1 cut(s) 949
SfcI CTRYAG 3 cut(s) 334, 659, 861
SfuI TTCGAA 1 cut(s) 1366
SmiMI CAYNNNNRTG 1 cut(s) 1469
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 9 cut(s) 24, 110, 219, 237, 354, 440, 801, 921, 1083
SsiI CCGC 2 cut(s) 206, 1574
SspMI CTAG 5 cut(s) 378, 780, 1103, 1422, 1505
StyD4I CCNGG 2 cut(s) 145, 813
TaaI ACNGT 5 cut(s) 89, 255, 624, 1270, 1346
TaqI TCGA 3 cut(s) 156, 1366, 1494
TasI AATT 9 cut(s) 24, 110, 219, 237, 354, 440, 801, 921, 1083
TatI WGTACW 2 cut(s) 1341, 1437
TauI GCSGC 1 cut(s) 1577
TfiI GAWTC 2 cut(s) 948, 1276
Tru1I TTAA 8 cut(s) 114, 222, 273, 435, 776, 1035, 1122, 1131
Tru9I TTAA 8 cut(s) 114, 222, 273, 435, 776, 1035, 1122, 1131
TscAI CASTG 1 cut(s) 533
TseFI GTSAC 1 cut(s) 447
TseI GCWGC 4 cut(s) 176, 203, 289, 826
Tsp45I GTSAC 1 cut(s) 447
TspDTI ATGAA 9 cut(s) 17, 123, 291, 317, 374, 501, 887, 1161, 1192
TspRI CASTG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 434
XapI RAATTY 1 cut(s) 110
XbaI TCTAGA 1 cut(s) 1504
XmiI GTMKAC 2 cut(s) 617, 1179
XmnI GAANNNNTTC 3 cut(s) 404, 471, 912
XspI CTAG 5 cut(s) 378, 780, 1103, 1422, 1505
ZrmI AGTACT 1 cut(s) 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.