Rh7AG292000

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006221
Physical Location & Seq
Forward (+)
31883282 .. 31885728
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG292000.1

Sequence Viewer

Length: 345 bp
ATGGATTTGAAACCATTTCTCATCTGTTTGGTCCGTTCATTTGTTGTTACATCCATATGCACCAACTGCGCCATTCTAGTATGGTTATATGATCATGAGGGGAAATGGAATATAGAGCCTGGCATAGCTGCAATTCTGGTTATGGAACATATCCTCCTGTTGATTAAGTTTGGTTTCTCTCGCCTTGTACCTGAGGTGATATTAAATCACTCCTGTGAACACTTGCTTACATCTCTTATCGCATTTAGAGTATGTAATTGTTGGAATATTTCTGGATATAGGGCAGCTGGTACTAGTGCTTGGTTAAGAAGGAAGAATGAGGTTGGAGGTGGAACGTTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

114

Amino Acids

12.92

Weight (kDa)

8.28

Isoelectric Point (pI)

58.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 14 - 66 3.1e-06 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 335
AfaI GTAC 2 cut(s) 189, 292
AgsI TTSAA 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 1 cut(s) 118
AleI CACNNNNGTG 1 cut(s) 213
AloI GAACNNNNNNTCC 2 cut(s) 138, 170
AluBI AGCT 2 cut(s) 128, 287
AluI AGCT 2 cut(s) 128, 287
ApeKI GCWGC 2 cut(s) 128, 284
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 31
AxyI CCTNAGG 1 cut(s) 192
BbvI GCAGC 2 cut(s) 115, 296
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 91
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 2 cut(s) 77, 294
BisI GCNGC 2 cut(s) 129, 285
BlsI GCNGC 2 cut(s) 130, 286
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse21I CCTNAGG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 50
BseMII CTCAG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 115, 296
Bsp143I GATC 1 cut(s) 91
BspCNI CTCAG 1 cut(s) 184
BspHI TCATGA 1 cut(s) 94
BssMI GATC 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 115, 296
Bsu36I CCTNAGG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 50
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 188, 291
CviAII CATG 1 cut(s) 95
CviJI RGCY 3 cut(s) 118, 128, 287
CviKI_1 RGCY 3 cut(s) 118, 128, 287
CviQI GTAC 2 cut(s) 188, 291
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 31
Eco81I CCTNAGG 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 98
FatI CATG 1 cut(s) 94
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 129, 285
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 129, 285
FspBI CTAG 2 cut(s) 77, 294
GlaI GCGC 1 cut(s) 70
GluI GCNGC 2 cut(s) 129, 285
HhaI GCGC 1 cut(s) 71
Hin1II CATG 1 cut(s) 98
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 218
Hpy188III TCNNGA 2 cut(s) 95, 273
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 303
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 2 cut(s) 60, 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 1 cut(s) 98
HspAI GCGC 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 1 cut(s) 91
LpnPI CCDG 8 cut(s) 105, 122, 132, 170, 204, 226, 258, 273
Lsp1109I GCAGC 2 cut(s) 115, 296
MaeI CTAG 2 cut(s) 77, 294
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 1 cut(s) 325
MluCI AATT 2 cut(s) 132, 256
MmeI TCCRAC 2 cut(s) 242, 304
MnlI CCTC 5 cut(s) 91, 164, 187, 313, 320
MseI TTAA 3 cut(s) 165, 203, 305
MslI CAYNNNNRTG 2 cut(s) 55, 213
MspA1I CMGCKG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 66
NdeI CATATG 1 cut(s) 56
NdeII GATC 1 cut(s) 91
NlaIII CATG 1 cut(s) 98
OliI CACNNNNGTG 1 cut(s) 213
PagI TCATGA 1 cut(s) 94
PkrI GCNGC 2 cut(s) 130, 286
Psp1406I AACGTT 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspPI GGNCC 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 2 cut(s) 189, 292
RsaNI GTAC 2 cut(s) 188, 291
RseI CAYNNNNRTG 2 cut(s) 55, 213
SaqAI TTAA 3 cut(s) 165, 203, 305
SatI GCNGC 2 cut(s) 129, 285
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 120
SetI ASST 7 cut(s) 130, 193, 198, 289, 324, 331, 338
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 2 cut(s) 55, 213
SpeI ACTAGT 1 cut(s) 293
Sse9I AATT 2 cut(s) 132, 256
SspI AATATT 1 cut(s) 268
SspMI CTAG 2 cut(s) 77, 294
StyD4I CCNGG 1 cut(s) 118
TaiI ACGT 1 cut(s) 338
TasI AATT 2 cut(s) 132, 256
Tru1I TTAA 3 cut(s) 165, 203, 305
Tru9I TTAA 3 cut(s) 165, 203, 305
TseI GCWGC 2 cut(s) 128, 284
TspDTI ATGAA 1 cut(s) 27
TspGWI ACGGA 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 31
XspI CTAG 2 cut(s) 77, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.