MD17G1178100.v1.1

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006221
Physical Location & Seq
Reverse (-)
20383855 .. 20388682
4828 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1178100.v1.1.491

Sequence Viewer

Length: 2109 bp
ATGCAATGGCCCACTTCGCCATTTGAGCCGGTTTCTGCAAAGTTCGCGTCTTTTGTTGATTTGGGCATCGTTTGGATCTCGAAATTTGACCCCTTCGGCTGCTGGGTGCTGAAGTTGTTTGGTGGTGTGCAGGAGATGAACGCCGTTGAGGAGGAACAGACTGCCTTCGAGATCGGGGTGGTGGTTCCGAAGCGTAATGTGAAGGAGGAAGATAGAGCTTTTGATTGTGTGGAGGTTTTGGTGGAGGAGTTTAGGAATTTGGGTTTGGTTGTTGAGAGAGTGCATGGCGTTGTCGATGAGTTCATCAAGCTGGCAGCACCTTTGGAGACGCTGGGCCGGGCTGCAGCGGAATTGCAGATTAAAAAACGAACTGCTATCGGGATGGATTTACAATTTGAATGGGAGGAGGTTGATGCTTTTGTGAGACAGCCTGATGGTTCAGTGTTCAGTTGGTGTGAGCGTTTTCGCTGCTATAGGCACTTAATATATGGAATTATTAACAAGAGCCGGTCAACTGTAACTTTAAGATTTGATGATAAAGAGTTCCGTTGGGAAGTCGGGGAGTATTTACTTCAGAAGTTGGAATCAGAAAGGATTGTCAAAGAAGTGTTTCCTTTGCATGATGAAATAATAAGGAAGAAGCTCCTTCACTCATGGGCACTACACTGGTGGGACTTCACAAACCAGCCGATAGATGAGATTTATTCATACTTTGGTACAAAGATCGCCATCTATTTTGCTTTCCTTGGAATGTATACAAGGTGGTTGCTCTTTCCAGCTGCATTTGGGCTTATCATGCACATGTTTGATTTTGGGTCATTGCAGTTACTGGTGCTCCCTATTTTCTTCATAAGCATAATATTGTGGGCTATAATGTTTTCACAGTTTTGGAAACGCAAAAATAATGCCCTTTTAGCCAGATGGCAGATTAGTTATCCAGTTAGAGCTGACCTGGGATATAGACAGGGTGTGGAATGGAGTTCCTCACCATCTGTTGAGCTTGCAAAAAAGTGGGGGACAGACAAAACAAAAGAAAAAGAAATGTTCCAAAGAATTGAGTGGTTTGGACGACTCATGAGATTCCGAAATGATGCTATCATTTTCTTCAGTATTATATGCCTTCAGTTACCATTTGAATTGGCATATGCTCATATTTATGAGGTTATTGAGTCTGATATTATGAAGTTTGGGTTGACGACCGTTTATCTTTTTTCAATTCAGTTCTTTACACAGATTGGAGGCAAGATCTCCGTCAAGCTCATTCAATATGAAAATAATGAAAACAAAGAAAAAAGAGCTGACAGCTTGGTCTATAAGGTGTTTGGTCTTTATTTCATGCAGTCATATATAGGAGTGTTCTATCATGCCCTTTTGCACCGCAACTTTTCTACTCTCCGCAAAGTCTTGATTCAACGCCTCCTTGTGTCTCAGGTGTTGGAAAACTTGTTGGAAAACACGTTGCCATATTTAAAGTACAGTTATAAGAAATATAGAGTTCAAAGCAAGAAAAAGTGTGAAAAAGGTTCAACGACAGGGAAGATTCGGTTTGCTTCTAGGGTAGAGAAAGAGTACCTGAAGCCTTCATATTCCGCTAGCATTGGCATGGAACTTGAAGATGGGCTGTTTGATGACTTTCTTGAGGTGGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCATTCCCCCTTGCTTTTGCCTTTGCAATTTTGAACAACATCACTGAAATTAGGGCGGATGCATTGAAGCTTCTTGCCATGTTGCGCAGACCAACTCCTCGCTTTTCCACAACAATTGGAGCTTGGCTTAACATATTTCAGTTTCTTGTAGTTATGTCTATATGCACCAACTGCGCACTTCTAGTATGGTTATATGATGAAGAGGGCAAATGGAAGATAGAGCCTGGCCTGGCTGCAATTCTGGTTATGGAACATGTTCTCTTGTTGATCAAATTTGGATTCTCCCGCTTTGTTCCCGAGGAACCTGCTTGGGTAAAAGCCAACCGTGTGAAAAACGCGACAACAGCTGAGAACATGTGTTCTAAACAGCTATTGAGGAGCATTTCTGGAGAGAGGATGACGTTTGGTAATGTCAAGAAGTCGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

703

Amino Acids

81.91

Weight (kDa)

8.72

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 234 - 670 7.1e-104 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1482
AasI GACNNNNNNGTC 1 cut(s) 1307
Acc16I TGCGCA 2 cut(s) 1766, 1855
Acc36I ACCTGC 1 cut(s) 1993
AccI GTMKAC 1 cut(s) 755
AccII CGCG 2 cut(s) 47, 2018
AciI CCGC 6 cut(s) 347, 1378, 1396, 1590, 1736, 1966
AclWI GGATC 1 cut(s) 83
AcsI RAATTY 3 cut(s) 83, 256, 1952
AcuI CTGAAG 5 cut(s) 131, 557, 1090, 1106, 1595
AfaI GTAC 3 cut(s) 718, 1475, 1571
AflIII ACRYGT 4 cut(s) 801, 1455, 1933, 2034
AjnI CCWGG 3 cut(s) 951, 1903, 1908
AjuI GAANNNNNNNTTGG 2 cut(s) 248, 280
Alw21I GWGCWC 1 cut(s) 837
Alw26I GTCTC 3 cut(s) 320, 418, 1431
AlwI GGATC 1 cut(s) 83
AlwNI CAGNNNCTG 1 cut(s) 829
Ama87I CYCGRG 1 cut(s) 1976
AoxI GGCC 3 cut(s) 8, 334, 1906
ApeKI GCWGC 7 cut(s) 99, 314, 341, 344, 468, 779, 1913
ApoI RAATTY 3 cut(s) 83, 256, 1952
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
Asp700I GAANNNNTTC 2 cut(s) 609, 1935
AspLEI GCGC 2 cut(s) 1767, 1856
AspS9I GGNCC 2 cut(s) 9, 334
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 978
AsuNHI GCTAGC 1 cut(s) 1592
AvaI CYCGRG 1 cut(s) 1976
BaeGI GKGCMC 1 cut(s) 661
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 7 cut(s) 86, 326, 328, 356, 455, 766, 1900
BccI CCATC 6 cut(s) 376, 428, 737, 915, 997, 1610
BceAI ACGGC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 1967, 2001
BciT130I CCWGG 3 cut(s) 953, 1905, 1910
BclI TGATCA 2 cut(s) 1662, 1947
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 3 cut(s) 320, 418, 1431
BfaI CTAG 3 cut(s) 1554, 1593, 1862
BfmI CTRYAG 2 cut(s) 342, 472
BfuAI ACCTGC 1 cut(s) 1993
BglII AGATCT 1 cut(s) 1245
BisI GCNGC 7 cut(s) 100, 315, 342, 345, 469, 780, 1914
BlsI GCNGC 7 cut(s) 101, 316, 343, 346, 470, 781, 1915
Bme1390I CCNGG 4 cut(s) 338, 953, 1905, 1910
BmeT110I CYCGRG 1 cut(s) 1976
BmgT120I GGNCC 2 cut(s) 9, 334
BmiI GGNNCC 2 cut(s) 186, 1983
BmrFI CCNGG 4 cut(s) 338, 953, 1905, 1910
BmsI GCATC 4 cut(s) 75, 403, 1081, 1729
BmtI GCTAGC 1 cut(s) 1596
BpmI CTGGAG 1 cut(s) 2088
BpuEI CTTGAG 1 cut(s) 1658
BpuMI CCSGG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 728
BsaJI CCNNGG 3 cut(s) 745, 952, 1977
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bse118I RCCGGY 2 cut(s) 28, 507
Bse1I ACTGG 3 cut(s) 671, 834, 938
Bse3DI GCAATG 3 cut(s) 11, 818, 1646
Bse8I GATNNNNATC 1 cut(s) 728
BseBI CCWGG 3 cut(s) 953, 1905, 1910
BseDI CCNNGG 3 cut(s) 745, 952, 1977
BseGI GGATG 3 cut(s) 387, 1744, 2082
BseJI GATNNNNATC 1 cut(s) 728
BseMI GCAATG 3 cut(s) 11, 818, 1646
BseMII CTCAG 2 cut(s) 1442, 2019
BseNI ACTGG 3 cut(s) 671, 834, 938
BseRI GAGGAG 5 cut(s) 164, 260, 419, 1767, 2071
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 7 cut(s) 86, 326, 328, 356, 455, 766, 1900
BseYI CCCAGC 2 cut(s) 102, 331
BsgI GTGCAG 1 cut(s) 149
Bsh1236I CGCG 2 cut(s) 47, 2018
Bsh1285I CGRYCG 1 cut(s) 1200
BshFI GGCC 3 cut(s) 10, 336, 1908
BsiEI CGRYCG 1 cut(s) 1200
BsiHKAI GWGCWC 1 cut(s) 837
BsiHKCI CYCGRG 1 cut(s) 1976
BsiSI CCGG 3 cut(s) 29, 337, 508
BslFI GGGAC 2 cut(s) 686, 1030
BsmAI GTCTC 3 cut(s) 320, 418, 1431
BsmBI CGTCTC 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 686, 1030
BsmI GAATGC 1 cut(s) 1682
BsnI GGCC 3 cut(s) 10, 336, 1908
BsoBI CYCGRG 1 cut(s) 1976
Bsp1286I GDGCHC 2 cut(s) 661, 837
Bsp143I GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
BspACI CCGC 6 cut(s) 347, 1378, 1396, 1590, 1736, 1966
BspANI GGCC 3 cut(s) 10, 336, 1908
BspCNI CTCAG 2 cut(s) 1441, 2020
BspFNI CGCG 2 cut(s) 47, 2018
BspHI TCATGA 2 cut(s) 1074, 1665
BspLI GGNNCC 2 cut(s) 186, 1983
BspMAI CTGCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 1993
BspOI GCTAGC 1 cut(s) 1596
BspPI GGATC 1 cut(s) 83
BsrDI GCAATG 3 cut(s) 11, 818, 1646
BsrFI RCCGGY 2 cut(s) 28, 507
BsrI ACTGG 3 cut(s) 671, 834, 938
BssAI RCCGGY 2 cut(s) 28, 507
BssECI CCNNGG 3 cut(s) 745, 952, 1977
BssMI GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
BssNAI GTATAC 1 cut(s) 756
BssT1I CCWWGG 1 cut(s) 745
Bst1107I GTATAC 1 cut(s) 756
Bst2UI CCWGG 3 cut(s) 953, 1905, 1910
Bst4CI ACNGT 5 cut(s) 517, 885, 1201, 1478, 2006
Bst6I CTCTTC 1 cut(s) 1875
BstAPI GCANNNNNTGC 1 cut(s) 1851
BstC8I GCNNGC 3 cut(s) 312, 1002, 1594
BstDEI CTNAG 2 cut(s) 1428, 2028
BstF5I GGATG 3 cut(s) 387, 1744, 2082
BstFNI CGCG 2 cut(s) 47, 2018
BstHHI GCGC 2 cut(s) 1767, 1856
BstKTI GATC 6 cut(s) 78, 174, 726, 1248, 1665, 1950
BstMAI GTCTC 3 cut(s) 320, 418, 1431
BstMBI GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
BstMCI CGRYCG 1 cut(s) 1200
BstMWI GCNNNNNNNGC 7 cut(s) 16, 25, 44, 796, 914, 1851, 2024
BstNI CCWGG 3 cut(s) 953, 1905, 1910
BstNSI RCATGY 3 cut(s) 805, 1937, 2038
BstSCI CCNGG 4 cut(s) 336, 951, 1903, 1908
BstSFI CTRYAG 2 cut(s) 342, 472
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 2 cut(s) 47, 2018
BstV1I GCAGC 7 cut(s) 86, 326, 328, 356, 455, 766, 1900
BstX2I RGATCY 2 cut(s) 75, 1245
BstYI RGATCY 2 cut(s) 75, 1245
BstZ17I GTATAC 1 cut(s) 756
BsuRI GGCC 3 cut(s) 10, 336, 1908
BtsCI GGATG 3 cut(s) 387, 1744, 2082
BtsIMutI CAGTG 3 cut(s) 447, 664, 1722
BveI ACCTGC 1 cut(s) 1993
Cac8I GCNNGC 3 cut(s) 312, 1002, 1594
CaiI CAGNNNCTG 1 cut(s) 829
CciI TCATGA 2 cut(s) 1074, 1665
CfoI GCGC 2 cut(s) 1767, 1856
Cfr10I RCCGGY 2 cut(s) 28, 507
Cfr13I GGNCC 2 cut(s) 9, 334
CseI GACGC 2 cut(s) 36, 337
Csp6I GTAC 3 cut(s) 717, 1474, 1570
CspCI CAANNNNNGTGG 2 cut(s) 1777, 1812
CviQI GTAC 3 cut(s) 717, 1474, 1570
DdeI CTNAG 2 cut(s) 1428, 2028
DpnI GATC 6 cut(s) 77, 173, 725, 1247, 1664, 1949
DpnII GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
DraI TTTAAA 1 cut(s) 1470
DrdI GACNNNNNNGTC 1 cut(s) 1307
DseDI GACNNNNNNGTC 1 cut(s) 1307
Eam1104I CTCTTC 1 cut(s) 1875
EarI CTCTTC 1 cut(s) 1875
EciI GGCGGA 1 cut(s) 1751
Eco130I CCWWGG 1 cut(s) 745
Eco57I CTGAAG 5 cut(s) 131, 557, 1090, 1106, 1595
Eco88I CYCGRG 1 cut(s) 1976
EcoRII CCWGG 3 cut(s) 951, 1903, 1908
EcoT14I CCWWGG 1 cut(s) 745
EcoT22I ATGCAT 1 cut(s) 1744
ErhI CCWWGG 1 cut(s) 745
Esp3I CGTCTC 1 cut(s) 320
FaqI GGGAC 2 cut(s) 686, 1030
FauI CCCGC 1 cut(s) 1973
FauNDI CATATG 1 cut(s) 1144
FbaI TGATCA 2 cut(s) 1662, 1947
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 7 cut(s) 100, 315, 342, 345, 469, 780, 1914
FokI GGATG 3 cut(s) 394, 1751, 2089
Fsp4HI GCNGC 7 cut(s) 100, 315, 342, 345, 469, 780, 1914
FspBI CTAG 3 cut(s) 1554, 1593, 1862
FspI TGCGCA 2 cut(s) 1766, 1855
GlaI GCGC 2 cut(s) 1766, 1855
GluI GCNGC 7 cut(s) 100, 315, 342, 345, 469, 780, 1914
GsaI CCCAGC 2 cut(s) 106, 335
GsuI CTGGAG 1 cut(s) 2088
HaeIII GGCC 3 cut(s) 10, 336, 1908
HapII CCGG 3 cut(s) 29, 337, 508
HgaI GACGC 2 cut(s) 36, 337
HhaI GCGC 2 cut(s) 1767, 1856
Hin6I GCGC 2 cut(s) 1765, 1854
HinP1I GCGC 2 cut(s) 1765, 1854
HincII GTYRAC 2 cut(s) 513, 1194
HindII GTYRAC 2 cut(s) 513, 1194
HindIII AAGCTT 1 cut(s) 1748
HinfI GANTC 7 cut(s) 584, 1071, 1080, 1169, 1408, 1540, 1959
HpaII CCGG 3 cut(s) 29, 337, 508
HphI GGTGA 1 cut(s) 978
Hpy166II GTNNAC 3 cut(s) 513, 756, 1194
Hpy188I TCNGA 5 cut(s) 189, 576, 589, 1085, 1174
Hpy8I GTNNAC 3 cut(s) 513, 756, 1194
HpyAV CCTTC 6 cut(s) 103, 175, 196, 656, 1130, 1590
HpyCH4III ACNGT 5 cut(s) 517, 885, 1201, 1478, 2006
HpyCH4IV ACGT 2 cut(s) 1457, 2081
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 25, 44, 796, 914, 1851, 2024
HpyF3I CTNAG 2 cut(s) 1428, 2028
HpySE526I ACGT 2 cut(s) 1457, 2081
HspAI GCGC 2 cut(s) 1765, 1854
Ksp22I TGATCA 2 cut(s) 1662, 1947
Kzo9I GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
LmnI GCTCC 4 cut(s) 648, 840, 1799, 2058
Lsp1109I GCAGC 7 cut(s) 86, 326, 328, 356, 455, 766, 1900
LweI GCATC 4 cut(s) 75, 403, 1081, 1729
MaeI CTAG 3 cut(s) 1554, 1593, 1862
MaeII ACGT 2 cut(s) 1457, 2081
MaeIII GTNAC 3 cut(s) 517, 825, 1125
MalI GATC 6 cut(s) 77, 173, 725, 1247, 1664, 1949
MboI GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
MboII GAAGA 8 cut(s) 221, 649, 838, 1096, 1549, 1625, 1892, 1906
MfeI CAATTG 1 cut(s) 1794
MflI RGATCY 2 cut(s) 75, 1245
MhlI GDGCHC 2 cut(s) 661, 837
MlyI GAGTC 2 cut(s) 1065, 1178
MmeI TCCRAC 3 cut(s) 561, 1416, 1428
Mph1103I ATGCAT 1 cut(s) 1744
MroXI GAANNNNTTC 2 cut(s) 609, 1935
MseI TTAA 6 cut(s) 360, 482, 498, 524, 1469, 1809
MslI CAYNNNNRTG 3 cut(s) 800, 1155, 1601
MspA1I CMGCKG 3 cut(s) 347, 779, 2027
MspI CCGG 3 cut(s) 29, 337, 508
MspR9I CCNGG 4 cut(s) 338, 953, 1905, 1910
MunI CAATTG 1 cut(s) 1794
Mva1269I GAATGC 1 cut(s) 1682
MvaI CCWGG 3 cut(s) 953, 1905, 1910
MvnI CGCG 2 cut(s) 47, 2018
MwoI GCNNNNNNNGC 7 cut(s) 16, 25, 44, 796, 914, 1851, 2024
NciI CCSGG 1 cut(s) 338
NdeI CATATG 1 cut(s) 1144
NdeII GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
NheI GCTAGC 1 cut(s) 1592
NlaIV GGNNCC 2 cut(s) 186, 1983
NsbI TGCGCA 2 cut(s) 1766, 1855
NsiI ATGCAT 1 cut(s) 1744
NspI RCATGY 3 cut(s) 805, 1937, 2038
PagI TCATGA 2 cut(s) 1074, 1665
PciI ACATGT 3 cut(s) 801, 1933, 2034
PctI GAATGC 1 cut(s) 1682
PdmI GAANNNNTTC 2 cut(s) 609, 1935
PfeI GAWTC 5 cut(s) 584, 1080, 1408, 1540, 1959
PkrI GCNGC 7 cut(s) 101, 316, 343, 346, 470, 781, 1915
PleI GAGTC 2 cut(s) 1065, 1177
PpsI GAGTC 2 cut(s) 1065, 1177
PscI ACATGT 3 cut(s) 801, 1933, 2034
PsiI TTATAA 1 cut(s) 1482
Psp6I CCWGG 3 cut(s) 951, 1903, 1908
PspFI CCCAGC 2 cut(s) 102, 331
PspGI CCWGG 3 cut(s) 951, 1903, 1908
PspN4I GGNNCC 2 cut(s) 186, 1983
PspPI GGNCC 2 cut(s) 9, 334
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 829
PsuI RGATCY 2 cut(s) 75, 1245
PvuII CAGCTG 2 cut(s) 779, 2027
RsaI GTAC 3 cut(s) 718, 1475, 1571
RsaNI GTAC 3 cut(s) 717, 1474, 1570
RseI CAYNNNNRTG 3 cut(s) 800, 1155, 1601
SaqAI TTAA 6 cut(s) 360, 482, 498, 524, 1469, 1809
SatI GCNGC 7 cut(s) 100, 315, 342, 345, 469, 780, 1914
Sau3AI GATC 6 cut(s) 75, 171, 723, 1245, 1662, 1947
Sau96I GGNCC 2 cut(s) 9, 334
SchI GAGTC 2 cut(s) 1065, 1178
ScrFI CCNGG 4 cut(s) 338, 953, 1905, 1910
SduI GDGCHC 2 cut(s) 661, 837
SfaNI GCATC 4 cut(s) 75, 403, 1081, 1729
SfcI CTRYAG 2 cut(s) 342, 472
SmiMI CAYNNNNRTG 3 cut(s) 800, 1155, 1601
SmlI CTYRAG 1 cut(s) 1637
SmoI CTYRAG 1 cut(s) 1637
SsiI CCGC 6 cut(s) 347, 1378, 1396, 1590, 1736, 1966
SspI AATATT 1 cut(s) 861
SspMI CTAG 3 cut(s) 1554, 1593, 1862
StyD4I CCNGG 4 cut(s) 336, 951, 1903, 1908
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 5 cut(s) 517, 885, 1201, 1478, 2006
TaiI ACGT 2 cut(s) 1460, 2084
TaqI TCGA 3 cut(s) 80, 168, 294
TatI WGTACW 1 cut(s) 1473
TfiI GAWTC 5 cut(s) 584, 1080, 1408, 1540, 1959
Tru1I TTAA 6 cut(s) 360, 482, 498, 524, 1469, 1809
Tru9I TTAA 6 cut(s) 360, 482, 498, 524, 1469, 1809
TscAI CASTG 3 cut(s) 447, 671, 1729
TseI GCWGC 7 cut(s) 99, 314, 341, 344, 468, 779, 1913
TspGWI ACGGA 2 cut(s) 536, 1240
TspRI CASTG 3 cut(s) 447, 671, 1729
XapI RAATTY 3 cut(s) 83, 256, 1952
XceI RCATGY 3 cut(s) 805, 1937, 2038
XmiI GTMKAC 1 cut(s) 755
XmnI GAANNNNTTC 2 cut(s) 609, 1935
XspI CTAG 3 cut(s) 1554, 1593, 1862
Zsp2I ATGCAT 1 cut(s) 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.