Rorug02G0371600

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006221
Physical Location & Seq
Forward (+)
47398021 .. 47410418
12398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0371600.1

Sequence Viewer

Length: 4710 bp
ATGACCGGCGATACCACTTTTTCTCCGACCAACCGGGCCTTGTCATTCACCATAGAAGAAGGTTCTCAAAACAGCGAGCAACTTCTGGAGGACGAAAACAATGATCTTGGGAATGATTGCGAGCAATTGTTTGAGATTGACGGGAATGAGCCTGAAAGTGAAAGAGATGATACAAGTGATCAGTTTTTTGAGATTGATAGCAATGACAATGAAAATGACAGAGGTGATCTAAATGAACAGCTATTTGATATTCAAGGCAATGACCATGAGATGAGCAGGAATGATGATACTGTAATTGATGGTCAAAATGGTGGATCTCAAGAAAAGGTTTATCCCCAACCAGTTGTGGGTTTGGAGTTTGATTCATATGATGATGCTTACAATTATTATAATTGCTATGCTAAGGAACTTGGATTTGCCATCAGGGTGAAATCTTCATGGACAAAGCGTAACAGCAAAGAGAAGCGGGGTGCAGTTCTATGTTGCAACTGTGAGGGTTTTAAAACAATCAAAGAAGCAAACAGTCGCAAGAAAGAAACGAGAACTGGTTGTCTAGCAATGATAAGGTTGAGACTAGTGGAATCTAACAGATGGAGGGTGGATGAAGTCAAGCTTGAACACAACCATTTATTCGATCCTGAAAGAGCTCAGAACTCCAAGTCCCACAAGAAGATGGATTCTGGGGCTAAAAGAAAGATGGAGCCAACCCTTGATGTTGAAGTACGCACGATCAAGTTATATCGCACACCTGGCGCAGACATAGTTGGTTATGGAAGCTCGAACTCCTATGAAGGAGAAAGTAACAGTTATGTTGATGTGTCCAAGAGATTGAAACTCAAACAAGGAGATGCAAGAGCAATTTATAAATATTTCAGTCGGGTACAGCTAACTGATCCTAATTTTTTCTATGTGAATGATCTCAGTGATGAAGGGCATTTGAGGAATGTGTTTTGGATTGATTCTAGGTCTAGAGCTGCATACAGTTACTTTGGTGATGTAGTAGCATTTGACACTACATGCTTGTCAAACAATTATGAGATTCCGCTTGTTGCATTTGTTGGAGTAAATCACCATGGGCAGACAATTTTGCTTGGTTGTGGTTTGCTTGCTGATGAGACAGTGGAAACATATATATGGTTATTTAGGGCATGGCTTACCTGTATGTCTGGTCGCCCTCCACAGACTATAATCACAAACCAGTGCAAGGCCATGCACAGTGCTATAGTAGAGGTATTTCCTAGGGCTCATCATCGTCTTTGTACTTCACATGTAGTGCAAAGTATTCTTGAGAATATGGAAGCACTGCAAGACTACGAGGCGTTTCAGATGATTTTGAGTAGAACTGTATACGACTCATTAAAAGCGGATGAGTTTGAATCGGGGTGGGAAGATATGATCCAGAGATTTGGAATTAGGAATCATGAATTTCTTCAAACTTTGTATGAAGATCGTGAACGTTGGGGTCCAGCTTACTCAAAGGACACATGTTTTGCTGGAATGCCCGATTTTCAAAATGGAGAGTCCACAAGGCCATATTTTGATGGTCACATTCATCAAAGAACTTCTTTGGAAGAGTTTTTCGAGGTTTATGATTTTGTTTTGGAAAATAAGCGTCAGGAAGAAGCTCGTAATGATTTAGTGTCCAGAGACTTGAACCCCATATTGAGAACAAGATGCTATTATGAGTTGCAGCTCTCCAAGTTCTACACAAAAGATGTATTCAGAAAGTTTCAACATGAGGTTGCAATGATGTCTTCCTGTTTTAGCATTACTCAAGTTCATACAAATGGACCAATAGTGACATACATGATCAAAGAACGTGAAGGGGAGGAAATTTTGAGGGGTGTTAGGAACTTTGAAGTTATGTATGACAAAGCGGGAGCAGAAGTTCGTTGCATGTGCAGTTGCCTTAATTTAAATGGGTACTTATGTCGACATGCCTTGTGTATCCTCAACTATAATGGTGTGGAAGAGATACCGTTTCAGTATATCTTTTCACGATGGAGGAAAGACTTTAAGCGCTTGTATGTACCAGATCTTGGCTGCAACAATGTTGATATCACGAACCCAGTTCAGTGGTTTGATCATTTGTATAGAAGAGCAATGCAAGTTGTTCAGGAAGGGATGATTTCTCAGGATCATTATATGGTATCTTGGCAGGCATTTAAAGAGTCTTTGAATAAGGTCCGTCTTGTAGCAGACAAGCAGGTCACTTGGACAGATGGAAGAAGAGAGTTGTCACTTGTCAGTGAACGTTTGGTGCCCACCTGCAAAGGTAGGCATGCTTGCAAAAGTTTCCCCTCACTGCGAGTCTGTTACCAATTCTTCTCTTCAGCTACTAATTCATTGCCTTCTGTATCGGATGGCTTTGGAACTGAGAACAAAAGAACTGATTTCGGTCTTCTATTTCAATCCTGCACAAAACTTCACCATGCCAAGTGTCTCCATGCTTTTCTTGTGGTGTCAGGAAAAGCTCAAGATATTATTTTTTCCTCCAGGCTAGTCAATCGGTATGCCTACCTTGGTGATGTTTCATTCTCACGCAGCACTTTTGATTCGATGCCTAGAAAGGATGTCTATACCTGGAATTCAATGGTGTCTGCATATGTTCGCAGTGGACGTTTCCGAGAAGCTGTAGACTGTTTCTCTGATTTTTTGTTGACCCCTGGTCTGCGACCTGATTTCTACACTTTTCCTCCTGTCTTGAAAGCGTGTGGGAATCTAAATGTTGGGAAGAAAATACACTGTTGGCTTATAAAGCTGGGTTTGGAATGGGATGTCTTTGTGGCTGCTTCCTTGATCCATATGTATTCACGGTTTGGATTTATTAGCGTTGCTCATAAATTGTTTGATGAGATGCCATTCCGGGATATGGGTTGTTGGAATGCAATGATTTCTGGGTTTTGTCAGAATGGGAATGCAGCAGATGCATTAGATGTCTTGACTGAAATGAGATCGGAGGGTGTAAAGATGGATCCTGTAACTGTTGCAAGTCTACTGACTGCTTGTGCACAATCAGATGATTCCTTAAGTGGGATGCTGATTCATTTGTATGTCATAAAGCATGGACTAGAGTTTGATCTGTTCGTATGCAATGCCTTGATTAACATGTATGCTAAATTTGGTTGCTTGGGACACGCACACAGGGTTTTTTATGAAATGCAGGTAAGAGATTTGGTGTCATGGAACTCAATAATTACTGCAAATGAGCAGAATGATGAGCCGATGACTGCACTTGGATTCTTCAACAGAATGCAACTATCCGGAGTTAAGCCTGATTGGTTAACACTAGTGAGTTTGGCTTCCATTATAGCTCAATTAAATGATGCTGAAATGAGTAGGTCTGTTCATGGATTCATTTTGAGGAGAGATTGGTTCAAGGAAGATGTTGTTATCGGAAATGCAGTTGTGGACATGTATGCAAAGTTGGGTGCTGTAGATTCTGCACGTACTGTTTTTGAAGGACTTCCTGTCAAGGATGTGGTTTCATGGAATACTTTGATCACAGGTTACACTCAAAATGCTCTTGCAAGTGAGGCGAATGAAGTGTACTGTATGATGCAAGAGTGCAAAGAAATAATTCCAAACCAAGGAACTTGGGTTAGCATTCTACCAGCATATACCCATTTAGGGGCTTTGCAACAAGGGATGAAAATTCATGGGCGGGTGATCAAGAACTGTCTCTACTTGGATGTATTTGTGGGTACCTGCTTGATTGACATGTATGGAAAATGTGGGAGACTAGATGATGCATTATCATTATTCTACCAAGTGCCCAGAATGAGTTCAGTCACTTGGAATGCCATGGTTTCCTGTCTTGGAGTTCATGGGCATGGTGGGAAAGTTGTGAAGCTATTCAAAGATATGATAGATGAGGGGGTCAAGCCAGATCATGTAACCTTTGTATCTCTAATGGCAGCTTGTAGTCATTCAGGTTTGGTCAAGGAGGGTGAATTGTACTTTCATATGATGCAGAAAGAGTATGGAATCAAGCCTGGTTTAAAGCACTACGGCTGCATGGTAGATTTACTTGGTAGAGCTGGGTATTTAGACAAGGCATATAGTTTTATTAAGACTATGCCTGTACAGCCTGATGCTTCTGTTTGGGGGGCACTACTTGGTGCTTGTAGAATACATGGGAATGCTGAATTGGGGAAAATTGCTTCAGAAGGCTTGTTTGAAGTTGATTCGGAGAATGTGGGCTACTATGTTTTGTTGTCAAATATATACGCAACAACCGGGAAATGGCAAGGTGTGGATAAAGTGAGATCTATGGCTAGAAACCGCGGACTGAGGAAGACTCCCGGGTGGAGCTCAATAGAAGTTAACAATAAAGTTGATGTCTTTTATACTGGGAACCAAACGCATCCATTATGCGAACAGATATACCAGAAGTTGAGGGAACTAACTGAGAAAATGAAGAGCCTTGGTTATGTTCCGGACTTCAGCTTTGTGTTGCAGGATGTCGAGAATGACGAGAAAGAGCATATCCTCACGAGTCATAGTGAGAGATTGGCGATTGCTTTTGGAATCATTAGCACGCCTCCTAAAACTCCGATTCGGATCTTCAAGAACCTGAGGGTTTGCGGTGATTGCCACAATGTGACGAAACTCATATCAGTAATTACGGAGAGGGAGATCATTGTGAGGGATTCAAACCGTTTCCACCATTTCAAGGATGGAACTTGCTCATGTGGGGATTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

1569

Amino Acids

178.98

Weight (kDa)

5.95

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 128 - 211 1.3e-23 FAR1 DNA-binding domain
MULE PF10551 333 - 426 8.4e-30 MULE transposase domain
PPR_2 PF13041 857 - 905 4.4e-06 PPR repeat family
PPR_2 PF13041 957 - 1004 1.4e-10 PPR repeat family
PPR_1 PF12854 957 - 985 3e-06 PPR repeat
PPR PF01535 960 - 989 2.2e-07 PPR repeat
TPR_24 PF23276 962 - 1052 5.6e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 1159 - 1206 1e-05 PPR repeat family
PPR_2 PF13041 1262 - 1309 3.3e-10 PPR repeat family
PPR PF01535 1264 - 1294 8.2e-06 PPR repeat
E_motif PF20431 1379 - 1441 5.5e-17 E motif
DYW_deaminase PF14432 1477 - 1569 6.5e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 390, 864, 2756
AarI CACCTGC 1 cut(s) 2276
AasI GACNNNNNNGTC 1 cut(s) 2207
Acc36I ACCTGC 4 cut(s) 2197, 2276, 3154, 3716
Acc65I GGTACC 1 cut(s) 3704
AccB1I GGYRCC 2 cut(s) 2260, 3704
AccB7I CCANNNNNTGG 1 cut(s) 2039
AccI GTMKAC 4 cut(s) 1347, 1933, 2637, 2995
AccII CGCG 1 cut(s) 4287
AccIII TCCGGA 2 cut(s) 3263, 4438
AciI CCGC 8 cut(s) 466, 1043, 1364, 1877, 3664, 4285, 4287, 4587
AclI AACGTT 2 cut(s) 1456, 2254
AclWI GGATC 9 cut(s) 322, 629, 887, 1390, 2145, 2794, 2969, 2982, 4571
AcsI RAATTY 5 cut(s) 1424, 1833, 2587, 3119, 3654
AcuI CTGAAG 3 cut(s) 2316, 4149, 4429
AdeI CACNNNGTG 1 cut(s) 4603
AfeI AGCGCT 1 cut(s) 2021
AflII CTTAAG 1 cut(s) 3028
AflIII ACRYGT 5 cut(s) 1267, 1484, 3108, 3414, 3720
AhdI GACNNNNNGTC 2 cut(s) 2667, 3470
AhlI ACTAGT 2 cut(s) 574, 3289
AjnI CCWGG 5 cut(s) 748, 2495, 2582, 2665, 3994
AjuI GAANNNNNNNTTGG 2 cut(s) 4133, 4165
AloI GAACNNNNNNTCC 4 cut(s) 644, 676, 1872, 1904
Alw21I GWGCWC 3 cut(s) 649, 3013, 4316
Alw26I GTCTC 6 cut(s) 565, 1109, 1641, 2447, 3686, 3733
Alw44I GTGCAC 1 cut(s) 3009
AlwI GGATC 9 cut(s) 322, 629, 887, 1390, 2145, 2794, 2969, 2982, 4571
Ama87I CYCGRG 1 cut(s) 4304
Aor13HI TCCGGA 2 cut(s) 3263, 4438
Aor51HI AGCGCT 1 cut(s) 2021
AoxI GGCC 3 cut(s) 36, 1206, 1529
ApaLI GTGCAC 1 cut(s) 3009
ApeKI GCWGC 8 cut(s) 974, 1690, 2043, 2544, 2789, 2921, 3917, 4014
ApoI RAATTY 5 cut(s) 1424, 1833, 2587, 3119, 3654
ArsI GACNNNNNNTTYG 4 cut(s) 1472, 1504, 4433, 4465
Asp700I GAANNNNTTC 5 cut(s) 1428, 3465, 3579, 3784, 3854
Asp718I GGTACC 1 cut(s) 3704
AspA2I CCTAGG 1 cut(s) 1238
AspLEI GCGC 2 cut(s) 755, 2022
AspS9I GGNCC 4 cut(s) 36, 1463, 1790, 2185
AsuC2I CCSGG 5 cut(s) 35, 2867, 4240, 4305, 4306
AvaI CYCGRG 1 cut(s) 4304
AvaII GGWCC 3 cut(s) 1463, 1790, 2185
AvrII CCTAGG 1 cut(s) 1238
AxyI CCTNAGG 1 cut(s) 4577
BaeGI GKGCMC 4 cut(s) 2265, 3013, 3777, 4114
BaeI ACNNNNGTAYC 4 cut(s) 1915, 1948, 3888, 3921
BamHI GGATCC 1 cut(s) 2974
BanI GGYRCC 2 cut(s) 2260, 3704
BanII GRGCYC 3 cut(s) 649, 1246, 4316
BauI CACGAG 1 cut(s) 4495
BbsI GAAGAC 3 cut(s) 1746, 2393, 4304
Bbv12I GWGCWC 3 cut(s) 649, 3013, 4316
BbvI GCAGC 8 cut(s) 961, 1702, 2030, 2556, 2776, 2933, 3929, 4001
BceAI ACGGC 1 cut(s) 4027
BciT130I CCWGG 5 cut(s) 750, 2497, 2584, 2667, 3996
BciVI GTATCC 1 cut(s) 1958
BclI TGATCA 5 cut(s) 178, 1809, 2083, 3501, 3669
BcnI CCSGG 5 cut(s) 35, 2867, 4240, 4305, 4306
BcoDI GTCTC 6 cut(s) 565, 1109, 1641, 2447, 3686, 3733
BcuI ACTAGT 2 cut(s) 574, 3289
BfmI CTRYAG 3 cut(s) 1221, 2634, 3435
BfoI RGCGCY 1 cut(s) 2023
BfrI CTTAAG 1 cut(s) 3028
BfuAI ACCTGC 4 cut(s) 2197, 2276, 3154, 3716
BfuI GTATCC 1 cut(s) 1958
BglII AGATCT 2 cut(s) 2035, 4268
BisI GCNGC 8 cut(s) 975, 1691, 2044, 2545, 2790, 2922, 3918, 4015
BlnI CCTAGG 1 cut(s) 1238
BlsI GCNGC 8 cut(s) 976, 1692, 2045, 2546, 2791, 2923, 3919, 4016
Bme18I GGWCC 3 cut(s) 1463, 1790, 2185
BmeRI GACNNNNNGTC 2 cut(s) 2667, 3470
BmeT110I CYCGRG 1 cut(s) 4304
BmgT120I GGNCC 4 cut(s) 36, 1463, 1790, 2185
BmiI GGNNCC 6 cut(s) 702, 1464, 2262, 2976, 3706, 4358
BmrI ACTGGG 2 cut(s) 2063, 4362
BmuI ACTGGG 2 cut(s) 2063, 4362
BpiI GAAGAC 3 cut(s) 1746, 2393, 4304
BplI GAGNNNNNCTC 2 cut(s) 4285, 4317
BpmI CTGGAG 2 cut(s) 107, 2479
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 4 cut(s) 303, 1307, 1758, 2460
BpuMI CCSGG 5 cut(s) 35, 2867, 4240, 4305, 4306
BsaAI YACGTR 1 cut(s) 3449
BsaBI GATNNNNATC 1 cut(s) 4562
BsaJI CCNNGG 9 cut(s) 1072, 1238, 2521, 2665, 3589, 3804, 4285, 4304, 4426
BsaWI WCCGGW 2 cut(s) 3263, 4438
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 1872, 1902, 2680, 2710
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 5 cut(s) 341, 550, 1198, 2069, 4357
Bse21I CCTNAGG 1 cut(s) 4577
Bse3DI GCAATG 8 cut(s) 208, 265, 564, 1752, 2109, 2345, 2895, 3100
Bse8I GATNNNNATC 1 cut(s) 4562
BseAI TCCGGA 2 cut(s) 3263, 4438
BseBI CCWGG 5 cut(s) 750, 2497, 2584, 2667, 3996
BseDI CCNNGG 9 cut(s) 1072, 1238, 2521, 2665, 3589, 3804, 4285, 4304, 4426
BseJI GATNNNNATC 1 cut(s) 4562
BseMI GCAATG 8 cut(s) 208, 265, 564, 1752, 2109, 2345, 2895, 3100
BseMII CTCAG 7 cut(s) 662, 934, 2147, 2367, 4283, 4401, 4568
BseNI ACTGG 5 cut(s) 341, 550, 1198, 2069, 4357
BseRI GAGGAG 1 cut(s) 3379
BseSI GKGCMC 4 cut(s) 2265, 3013, 3777, 4114
BseXI GCAGC 8 cut(s) 961, 1702, 2030, 2556, 2776, 2933, 3929, 4001
BseYI CCCAGC 2 cut(s) 2761, 4040
BsgI GTGCAG 5 cut(s) 492, 1921, 2401, 3216, 3429
Bsh1236I CGCG 1 cut(s) 4287
BshFI GGCC 3 cut(s) 38, 1208, 1531
BshNI GGYRCC 2 cut(s) 2260, 3704
BsiHKAI GWGCWC 3 cut(s) 649, 3013, 4316
BsiHKCI CYCGRG 1 cut(s) 4304
BsiSI CCGG 7 cut(s) 6, 34, 2866, 3264, 4239, 4305, 4439
BslFI GGGAC 2 cut(s) 646, 3147
BsmAI GTCTC 6 cut(s) 565, 1109, 1641, 2447, 3686, 3733
BsmFI GGGAC 2 cut(s) 646, 3147
BsmI GAATGC 7 cut(s) 1503, 2890, 2923, 3258, 3606, 3805, 4147
BsnI GGCC 3 cut(s) 38, 1208, 1531
BsoBI CYCGRG 1 cut(s) 4304
Bsp1286I GDGCHC 7 cut(s) 649, 1246, 2265, 3013, 3777, 4114, 4316
Bsp13I TCCGGA 2 cut(s) 3263, 4438
Bsp1407I TGTACA 1 cut(s) 4084
Bsp19I CCATGG 2 cut(s) 1072, 3804
BspACI CCGC 8 cut(s) 466, 1043, 1364, 1877, 3664, 4285, 4287, 4587
BspANI GGCC 3 cut(s) 38, 1208, 1531
BspCNI CTCAG 7 cut(s) 661, 933, 2146, 2368, 4284, 4402, 4569
BspEI TCCGGA 2 cut(s) 3263, 4438
BspFNI CGCG 1 cut(s) 4287
BspHI TCATGA 1 cut(s) 1420
BspLI GGNNCC 6 cut(s) 702, 1464, 2262, 2976, 3706, 4358
BspMI ACCTGC 4 cut(s) 2197, 2276, 3154, 3716
BspPI GGATC 9 cut(s) 322, 629, 887, 1390, 2145, 2794, 2969, 2982, 4571
BspQI GCTCTTC 2 cut(s) 2092, 4415
BspT107I GGYRCC 2 cut(s) 2260, 3704
BspTI CTTAAG 1 cut(s) 3028
BsrDI GCAATG 8 cut(s) 208, 265, 564, 1752, 2109, 2345, 2895, 3100
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 4084
BsrI ACTGG 5 cut(s) 341, 550, 1198, 2069, 4357
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 9 cut(s) 1072, 1238, 2521, 2665, 3589, 3804, 4285, 4304, 4426
BssNAI GTATAC 1 cut(s) 1348
BssSI CACGAG 1 cut(s) 4495
BssT1I CCWWGG 6 cut(s) 1072, 1238, 2521, 3589, 3804, 4426
Bst1107I GTATAC 1 cut(s) 1348
Bst2BI CACGAG 1 cut(s) 4495
Bst2UI CCWGG 5 cut(s) 750, 2497, 2584, 2667, 3996
Bst6I CTCTTC 6 cut(s) 1566, 1965, 2092, 2224, 2335, 4415
BstAFI CTTAAG 1 cut(s) 3028
BstAPI GCANNNNNTGC 1 cut(s) 2927
BstAUI TGTACA 1 cut(s) 4084
BstBAI YACGTR 1 cut(s) 3449
BstC8I GCNNGC 7 cut(s) 77, 122, 1107, 2160, 2283, 2287, 4541
BstDEI CTNAG 8 cut(s) 402, 648, 920, 2133, 2376, 4292, 4410, 4577
BstDSI CCRYGG 3 cut(s) 1072, 3804, 4285
BstFNI CGCG 1 cut(s) 4287
BstH2I RGCGCY 1 cut(s) 2023
BstHHI GCGC 2 cut(s) 755, 2022
BstMAI GTCTC 6 cut(s) 565, 1109, 1641, 2447, 3686, 3733
BstMWI GCNNNNNNNGC 6 cut(s) 750, 2758, 2927, 3536, 4087, 4593
BstNI CCWGG 5 cut(s) 750, 2497, 2584, 2667, 3996
BstNSI RCATGY 9 cut(s) 1020, 1271, 1488, 1900, 1940, 2285, 3112, 3418, 3724
BstSFI CTRYAG 3 cut(s) 1221, 2634, 3435
BstSLI GKGCMC 4 cut(s) 2265, 3013, 3777, 4114
BstUI CGCG 1 cut(s) 4287
BstV1I GCAGC 8 cut(s) 961, 1702, 2030, 2556, 2776, 2933, 3929, 4001
BstV2I GAAGAC 3 cut(s) 1746, 2393, 4304
BstX2I RGATCY 5 cut(s) 314, 2035, 2974, 4268, 4563
BstXI CCANNNNNNTGG 2 cut(s) 1406, 2076
BstYI RGATCY 5 cut(s) 314, 2035, 2974, 4268, 4563
BstZ17I GTATAC 1 cut(s) 1348
Bsu36I CCTNAGG 1 cut(s) 4577
BsuI GTATCC 1 cut(s) 1958
BsuRI GGCC 3 cut(s) 38, 1208, 1531
BtgI CCRYGG 3 cut(s) 1072, 3804, 4285
BtsI GCAGTG 3 cut(s) 1301, 2303, 2620
BveI ACCTGC 4 cut(s) 2197, 2276, 3154, 3716
Cac8I GCNNGC 7 cut(s) 77, 122, 1107, 2160, 2283, 2287, 4541
CciI TCATGA 1 cut(s) 1420
CfoI GCGC 2 cut(s) 755, 2022
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 4 cut(s) 36, 1463, 1790, 2185
Cfr42I CCGCGG 1 cut(s) 4288
Cfr9I CCCGGG 1 cut(s) 4304
CseI GACGC 1 cut(s) 1601
DdeI CTNAG 8 cut(s) 402, 648, 920, 2133, 2376, 4292, 4410, 4577
DraI TTTAAA 4 cut(s) 502, 1917, 2167, 4002
DraIII CACNNNGTG 1 cut(s) 4603
DrdI GACNNNNNNGTC 1 cut(s) 2207
DriI GACNNNNNGTC 2 cut(s) 2667, 3470
DseDI GACNNNNNNGTC 1 cut(s) 2207
Eam1104I CTCTTC 6 cut(s) 1566, 1965, 2092, 2224, 2335, 4415
Eam1105I GACNNNNNGTC 2 cut(s) 2667, 3470
EarI CTCTTC 6 cut(s) 1566, 1965, 2092, 2224, 2335, 4415
Ecl136II GAGCTC 2 cut(s) 647, 4314
Eco130I CCWWGG 6 cut(s) 1072, 1238, 2521, 3589, 3804, 4426
Eco24I GRGCYC 3 cut(s) 649, 1246, 4316
Eco32I GATATC 1 cut(s) 2059
Eco47I GGWCC 3 cut(s) 1463, 1790, 2185
Eco47III AGCGCT 1 cut(s) 2021
Eco53kI GAGCTC 2 cut(s) 647, 4314
Eco57I CTGAAG 3 cut(s) 2316, 4149, 4429
Eco81I CCTNAGG 1 cut(s) 4577
Eco88I CYCGRG 1 cut(s) 4304
EcoICRI GAGCTC 2 cut(s) 647, 4314
EcoRI GAATTC 1 cut(s) 2587
EcoRII CCWGG 5 cut(s) 748, 2495, 2582, 2665, 3994
EcoRV GATATC 1 cut(s) 2059
EcoT14I CCWWGG 6 cut(s) 1072, 1238, 2521, 3589, 3804, 4426
EcoT22I ATGCAT 2 cut(s) 2932, 3754
EcoT38I GRGCYC 3 cut(s) 649, 1246, 4316
ErhI CCWWGG 6 cut(s) 1072, 1238, 2521, 3589, 3804, 4426
FalI AAGNNNNNCTT 4 cut(s) 597, 629, 1549, 1581
FaqI GGGAC 2 cut(s) 646, 3147
FauI CCCGC 3 cut(s) 459, 1870, 3657
FauNDI CATATG 4 cut(s) 367, 2605, 2805, 3966
FbaI TGATCA 5 cut(s) 178, 1809, 2083, 3501, 3669
FblI GTMKAC 4 cut(s) 1347, 1933, 2637, 2995
Fnu4HI GCNGC 8 cut(s) 975, 1691, 2044, 2545, 2790, 2922, 3918, 4015
FriOI GRGCYC 3 cut(s) 649, 1246, 4316
Fsp4HI GCNGC 8 cut(s) 975, 1691, 2044, 2545, 2790, 2922, 3918, 4015
GlaI GCGC 2 cut(s) 754, 2021
GluI GCNGC 8 cut(s) 975, 1691, 2044, 2545, 2790, 2922, 3918, 4015
GsaI CCCAGC 2 cut(s) 2765, 4044
GsuI CTGGAG 2 cut(s) 107, 2479
HaeII RGCGCY 1 cut(s) 2023
HaeIII GGCC 3 cut(s) 38, 1208, 1531
HapII CCGG 7 cut(s) 6, 34, 2866, 3264, 4239, 4305, 4439
HgaI GACGC 1 cut(s) 1601
HhaI GCGC 2 cut(s) 755, 2022
Hin6I GCGC 2 cut(s) 753, 2020
HinP1I GCGC 2 cut(s) 753, 2020
HincII GTYRAC 4 cut(s) 1934, 2661, 3285, 4327
HindII GTYRAC 4 cut(s) 1934, 2661, 3285, 4327
HindIII AAGCTT 1 cut(s) 611
HpaI GTTAAC 2 cut(s) 3285, 4327
HpaII CCGG 7 cut(s) 6, 34, 2866, 3264, 4239, 4305, 4439
HpyAV CCTTC 8 cut(s) 53, 785, 923, 1817, 2114, 2361, 3455, 4163
HpyCH4IV ACGT 5 cut(s) 1456, 1819, 2254, 2620, 3448
HpyF10VI GCNNNNNNNGC 6 cut(s) 750, 2758, 2927, 3536, 4087, 4593
HpyF3I CTNAG 8 cut(s) 402, 648, 920, 2133, 2376, 4292, 4410, 4577
HpySE526I ACGT 5 cut(s) 1456, 1819, 2254, 2620, 3448
HspAI GCGC 2 cut(s) 753, 2020
Kpn2I TCCGGA 2 cut(s) 3263, 4438
KpnI GGTACC 1 cut(s) 3708
Ksp22I TGATCA 5 cut(s) 178, 1809, 2083, 3501, 3669
KspAI GTTAAC 2 cut(s) 3285, 4327
KspI CCGCGG 1 cut(s) 4288
LguI GCTCTTC 2 cut(s) 2092, 4415
LmnI GCTCC 3 cut(s) 700, 1880, 4311
Lsp1109I GCAGC 8 cut(s) 961, 1702, 2030, 2556, 2776, 2933, 3929, 4001
MaeII ACGT 5 cut(s) 1456, 1819, 2254, 2620, 3448
MfeI CAATTG 1 cut(s) 125
MflI RGATCY 5 cut(s) 314, 2035, 2974, 4268, 4563
MhlI GDGCHC 7 cut(s) 649, 1246, 2265, 3013, 3777, 4114, 4316
MlyI GAGTC 6 cut(s) 1346, 1529, 2180, 2319, 4294, 4507
MmeI TCCRAC 3 cut(s) 50, 1039, 2861
Mph1103I ATGCAT 2 cut(s) 2932, 3754
MroI TCCGGA 2 cut(s) 3263, 4438
MroXI GAANNNNTTC 5 cut(s) 1428, 3465, 3579, 3784, 3854
MslI CAYNNNNRTG 6 cut(s) 425, 1132, 1785, 3051, 3831, 4140
MspA1I CMGCKG 1 cut(s) 4287
MspCI CTTAAG 1 cut(s) 3028
MspI CCGG 7 cut(s) 6, 34, 2866, 3264, 4239, 4305, 4439
MunI CAATTG 1 cut(s) 125
Mva1269I GAATGC 7 cut(s) 1503, 2890, 2923, 3258, 3606, 3805, 4147
MvaI CCWGG 5 cut(s) 750, 2497, 2584, 2667, 3996
MvnI CGCG 1 cut(s) 4287
MwoI GCNNNNNNNGC 6 cut(s) 750, 2758, 2927, 3536, 4087, 4593
NciI CCSGG 5 cut(s) 35, 2867, 4240, 4305, 4306
NcoI CCATGG 2 cut(s) 1072, 3804
NdeI CATATG 4 cut(s) 367, 2605, 2805, 3966
NlaIV GGNNCC 6 cut(s) 702, 1464, 2262, 2976, 3706, 4358
NmuCI GTSAC 6 cut(s) 1544, 1798, 2209, 2238, 3790, 4603
NsiI ATGCAT 2 cut(s) 2932, 3754
NspI RCATGY 9 cut(s) 1020, 1271, 1488, 1900, 1940, 2285, 3112, 3418, 3724
PaeI GCATGC 1 cut(s) 2285
PagI TCATGA 1 cut(s) 1420
PaqCI CACCTGC 1 cut(s) 2276
PciI ACATGT 5 cut(s) 1267, 1484, 3108, 3414, 3720
PciSI GCTCTTC 2 cut(s) 2092, 4415
PcsI WCGNNNNNNNCGW 2 cut(s) 2617, 4473
PctI GAATGC 7 cut(s) 1503, 2890, 2923, 3258, 3606, 3805, 4147
PdmI GAANNNNTTC 5 cut(s) 1428, 3465, 3579, 3784, 3854
PflMI CCANNNNNTGG 1 cut(s) 2039
PfoI TCCNGGA 1 cut(s) 2865
PkrI GCNGC 8 cut(s) 976, 1692, 2045, 2546, 2791, 2923, 3919, 4016
PleI GAGTC 6 cut(s) 1346, 1528, 2179, 2318, 4294, 4506
PpsI GAGTC 6 cut(s) 1346, 1528, 2179, 2318, 4294, 4506
Ppu21I YACGTR 1 cut(s) 3449
PscI ACATGT 5 cut(s) 1267, 1484, 3108, 3414, 3720
PsiI TTATAA 3 cut(s) 390, 864, 2756
Psp124BI GAGCTC 2 cut(s) 649, 4316
Psp1406I AACGTT 2 cut(s) 1456, 2254
Psp6I CCWGG 5 cut(s) 748, 2495, 2582, 2665, 3994
PspFI CCCAGC 2 cut(s) 2761, 4040
PspGI CCWGG 5 cut(s) 748, 2495, 2582, 2665, 3994
PspN4I GGNNCC 6 cut(s) 702, 1464, 2262, 2976, 3706, 4358
PspPI GGNCC 4 cut(s) 36, 1463, 1790, 2185
PsrI GAACNNNNNNTAC 2 cut(s) 3668, 3700
PsuI RGATCY 5 cut(s) 314, 2035, 2974, 4268, 4563
RseI CAYNNNNRTG 6 cut(s) 425, 1132, 1785, 3051, 3831, 4140
SacI GAGCTC 2 cut(s) 649, 4316
SacII CCGCGG 1 cut(s) 4288
SalI GTCGAC 1 cut(s) 1932
SapI GCTCTTC 2 cut(s) 2092, 4415
SatI GCNGC 8 cut(s) 975, 1691, 2044, 2545, 2790, 2922, 3918, 4015
Sau96I GGNCC 4 cut(s) 36, 1463, 1790, 2185
SchI GAGTC 6 cut(s) 1346, 1529, 2180, 2319, 4294, 4507
SduI GDGCHC 7 cut(s) 649, 1246, 2265, 3013, 3777, 4114, 4316
SfcI CTRYAG 3 cut(s) 1221, 2634, 3435
Sfr303I CCGCGG 1 cut(s) 4288
SgrBI CCGCGG 1 cut(s) 4288
SinI GGWCC 3 cut(s) 1463, 1790, 2185
SmaI CCCGGG 1 cut(s) 4306
SmiI ATTTAAAT 1 cut(s) 1917
SmiMI CAYNNNNRTG 6 cut(s) 425, 1132, 1785, 3051, 3831, 4140
SmlI CTYRAG 5 cut(s) 318, 1286, 1773, 2475, 3028
SmoI CTYRAG 5 cut(s) 318, 1286, 1773, 2475, 3028
SpeI ACTAGT 2 cut(s) 574, 3289
SphI GCATGC 1 cut(s) 2285
SsiI CCGC 8 cut(s) 466, 1043, 1364, 1877, 3664, 4285, 4287, 4587
SspI AATATT 1 cut(s) 869
SstI GAGCTC 2 cut(s) 649, 4316
StyI CCWWGG 6 cut(s) 1072, 1238, 2521, 3589, 3804, 4426
SwaI ATTTAAAT 1 cut(s) 1917
TaiI ACGT 5 cut(s) 1459, 1822, 2257, 2623, 3451
TaqI TCGA 6 cut(s) 633, 779, 1581, 1933, 2558, 4467
TaqII GACCGA 1 cut(s) 2387
TatI WGTACW 4 cut(s) 1259, 3549, 3957, 4084
TseFI GTSAC 6 cut(s) 1544, 1798, 2209, 2238, 3790, 4603
TseI GCWGC 8 cut(s) 974, 1690, 2043, 2544, 2789, 2921, 3917, 4014
Tsp45I GTSAC 6 cut(s) 1544, 1798, 2209, 2238, 3790, 4603
TspGWI ACGGA 2 cut(s) 2177, 4643
TspMI CCCGGG 1 cut(s) 4304
Van91I CCANNNNNTGG 1 cut(s) 2039
Vha464I CTTAAG 1 cut(s) 3028
VneI GTGCAC 1 cut(s) 3009
VpaK11BI GGWCC 3 cut(s) 1463, 1790, 2185
XapI RAATTY 5 cut(s) 1424, 1833, 2587, 3119, 3654
XbaI TCTAGA 1 cut(s) 968
XceI RCATGY 9 cut(s) 1020, 1271, 1488, 1900, 1940, 2285, 3112, 3418, 3724
XcmI CCANNNNNNNNNTGG 1 cut(s) 4676
XmaI CCCGGG 1 cut(s) 4304
XmaJI CCTAGG 1 cut(s) 1238
XmiI GTMKAC 4 cut(s) 1347, 1933, 2637, 2995
XmnI GAANNNNTTC 5 cut(s) 1428, 3465, 3579, 3784, 3854
Zsp2I ATGCAT 2 cut(s) 2932, 3754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.