MD09G1196700.v1.1

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006221
Physical Location & Seq
Reverse (-)
17928311 .. 17933813
5503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1196700.v1.1.491

Sequence Viewer

Length: 2004 bp
ATGGTGAAGTTGTTTGGTGGTGGGCAGGAGATGAACGTCTTCGAGGAGGAACAAACTGTTTTCGAGATTGGGGTGGTGGTTCCGAAGCGAAATGTGAAAGAGGAAGATAAAACTTCTGATTGTGTGGAGGTTTTGGTGGAGGAGTTCAGGAATTTGGGTTTGGTTGTTGAGAGAGTGCACGGCGTTGTGGATGAGTTCATCAAGCTGGCAGCACCTTTGGAGACGCTGGGGAGGGCTGCAGCTGAGTTGCAGATTAAAAAGCGGACTGCTATCGGGATGGATTTACAATTTGAATGGGAGGAGGTTGATGCTTTTGTGAGGCAGCCTGATGGTTCGGTCTTCAGTTGGTGTGAGCGTTTTCGCTGCTATAGGCACTTAATATATGGAATTATTAACAAGAGCCGGTCAAGTGTAACTTTAAGATTTGATGATAAAGAATTTTGTTGGGAAGTCGGGGAGTATTTACTTCAGAAGTTGGAGTCAGAGAGGATTGTCAAAGAAGTGTTTCCTTTGCATGATGAAATAAAAAGGAAGAAGCTCCTTCACACGTGGGCACTACACTGGTGGGACTTCACAAACCAGCCAATAGATGAGATTTATGCATACTTTGGTGCAAAGATCGCCATCTATTTTGCTTTCCTTGGAATGTATACAAGGTGGTTGCTTTTTCCAGCTGCATTTGGGCTTATCATGCACATGTTTGATTTTGGGTCATTGCAGTTACTAGTGCTTCCCATTTTCTTCGTATGCATAATATTATGGGCTATAATGTTTTCACAGTTTTGGAAACGTAAAAATAGTGCCCTCTTAGCCAGATGGCAGATTAGTTATCCAGTTAGAACTGACTTGGGGTATAGACAAGGTGTGGGGTGGAGTTCCTCACCATCTGTTGAGCTCGCAAAAAAATGGGGGACCGACAAAACAAAAGAAAAAGAAATGTTCCAAAGAATTGAGTGGTTTGGACGACTCATGAGATTCCGAAATGATGCTTTCATTATCTTCAGTATTATATGCCTTCAGTTACCATTTGAACTGGCATATGCTCATATTTATGAGGTTATTGAATCTGATATTATGAAGGTTGGGTTGACTACCATTTATCTTTTTGCCATTCAGTTCTTGACACAGATTGGTGGCAAGATCTCCGTTAAGCTCATTAAATATGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCGTATATAGGAGTTTTCTACCATGCCCTTTTGCACCGCAACTTTTCTACTCTCCGCAAAGTTTTGATTCAACGCCTCCTTGTGTCTCAGGTGTTGGAAAACTTGTTGGAGAACATTTTGCCATATTTGAAGTACAGTTATAAGAAATATAGAGTTCGAAGCAAGAAAAAACGTGAAAGGGGTTCATCAATAGGGAAGATCCAGTTTGCTTACAGGGTAGAAAAGGAGTACCTGAAGCCTTCTTATTCTGCAAGCATTGGTATGGAACTTGAAGACGGGCTGTTTGATGACTTTCTGGAGGTGGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCATTCCCCCTTGCTTTTGCTTTCGCAACTTTGAACAATATCACTGAAATTAGGGCTGATGCGTTGAAGCTTCTTGCCATGTTGAGAAGACCAACTCCTCGATTTTCCACAACAATTGGAGCTTGGCTGAACATATTTCAGTTTCTCATAGTTATGTCTATATGCACCAACTGTGCACTGCTGGTATGGTTATATGATGAAGAGGGCAAATGGAAGATAGAGCCTGGCCTGGCTGCAATATTGGTTATGGAACATGTCCTCTTGTTGATCAAATTTGGTTTCTCCCGCTTTGTTCCCGAGGAACCCGCTTGGGTAGAAGCCAACCGTGTGAAAAACGCAACAAATGCTGAGAACATGTGCTCTAAACAGCTCTTGAAGAGTATATCTGGAGCAAGGACGACATTTGGTGATGTTAAGAAGTCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

668

Amino Acids

77.74

Weight (kDa)

8.79

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 199 - 635 3.8e-104 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1377
AasI GACNNNNNNGTC 1 cut(s) 1202
AccI GTMKAC 2 cut(s) 650, 1206
AciI CCGC 5 cut(s) 262, 1273, 1291, 1861, 1881
AclWI GGATC 1 cut(s) 1429
AcsI RAATTY 3 cut(s) 151, 437, 1847
AcuI CTGAAG 5 cut(s) 325, 452, 985, 1001, 1490
AcvI CACGTG 1 cut(s) 549
AfaI GTAC 2 cut(s) 1370, 1466
AflIII ACRYGT 4 cut(s) 546, 696, 1828, 1929
AgsI TTSAA 9 cut(s) 293, 1031, 1064, 1307, 1366, 1508, 1609, 1642, 1951
AhlI ACTAGT 1 cut(s) 724
AjnI CCWGG 2 cut(s) 1798, 1803
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
Alw21I GWGCWC 4 cut(s) 180, 897, 1753, 1937
Alw26I GTCTC 2 cut(s) 215, 1326
Alw44I GTGCAC 2 cut(s) 176, 1749
AlwI GGATC 1 cut(s) 1429
Ama87I CYCGRG 1 cut(s) 1871
AoxI GGCC 1 cut(s) 1801
ApaLI GTGCAC 2 cut(s) 176, 1749
ApeKI GCWGC 7 cut(s) 209, 236, 239, 322, 363, 674, 1808
ApoI RAATTY 3 cut(s) 151, 437, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 1502, 1534
Asp700I GAANNNNTTC 2 cut(s) 38, 504
AspS9I GGNCC 1 cut(s) 912
AsuHPI GGTGA 3 cut(s) 16, 873, 1994
AsuII TTCGAA 1 cut(s) 1393
AvaI CYCGRG 1 cut(s) 1871
AvaII GGWCC 1 cut(s) 912
BaeGI GKGCMC 4 cut(s) 180, 556, 805, 1753
BanII GRGCYC 1 cut(s) 897
BbrPI CACGTG 1 cut(s) 549
BbsI GAAGAC 4 cut(s) 31, 331, 1515, 1669
Bbv12I GWGCWC 4 cut(s) 180, 897, 1753, 1937
BbvI GCAGC 7 cut(s) 221, 223, 251, 334, 350, 661, 1795
BccI CCATC 5 cut(s) 271, 323, 632, 810, 892
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 2 cut(s) 1800, 1805
BclI TGATCA 2 cut(s) 1557, 1842
BcoDI GTCTC 2 cut(s) 215, 1326
BcuI ACTAGT 1 cut(s) 724
BfaI CTAG 1 cut(s) 725
BfmI CTRYAG 2 cut(s) 237, 367
BglII AGATCT 1 cut(s) 1140
BisI GCNGC 7 cut(s) 210, 237, 240, 323, 364, 675, 1809
BlsI GCNGC 7 cut(s) 211, 238, 241, 324, 365, 676, 1810
Bme1390I CCNGG 2 cut(s) 1800, 1805
Bme18I GGWCC 1 cut(s) 912
BmeT110I CYCGRG 1 cut(s) 1871
BmgT120I GGNCC 1 cut(s) 912
BmiI GGNNCC 3 cut(s) 81, 913, 1878
BmrFI CCNGG 2 cut(s) 1800, 1805
BmsI GCATC 3 cut(s) 298, 976, 1624
BpiI GAAGAC 4 cut(s) 31, 331, 1515, 1669
BpmI CTGGAG 2 cut(s) 1553, 1983
Bpu14I TTCGAA 1 cut(s) 1393
BsaAI YACGTR 1 cut(s) 549
BsaBI GATNNNNATC 1 cut(s) 623
BsaJI CCNNGG 2 cut(s) 640, 1872
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 4 cut(s) 566, 833, 1038, 1438
Bse3DI GCAATG 2 cut(s) 713, 1541
Bse8I GATNNNNATC 1 cut(s) 623
BseBI CCWGG 2 cut(s) 1800, 1805
BseDI CCNNGG 2 cut(s) 640, 1872
BseGI GGATG 2 cut(s) 196, 282
BseJI GATNNNNATC 1 cut(s) 623
BseMI GCAATG 2 cut(s) 713, 1541
BseMII CTCAG 3 cut(s) 234, 1337, 1914
BseNI ACTGG 4 cut(s) 566, 833, 1038, 1438
BseRI GAGGAG 4 cut(s) 59, 155, 314, 1662
BseSI GKGCMC 4 cut(s) 180, 556, 805, 1753
BseXI GCAGC 7 cut(s) 221, 223, 251, 334, 350, 661, 1795
BseYI CCCAGC 1 cut(s) 226
BshFI GGCC 1 cut(s) 1803
BsiHKAI GWGCWC 4 cut(s) 180, 897, 1753, 1937
BsiHKCI CYCGRG 1 cut(s) 1871
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 2 cut(s) 581, 925
BsmAI GTCTC 2 cut(s) 215, 1326
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 581, 925
BsmI GAATGC 1 cut(s) 1577
BsnI GGCC 1 cut(s) 1803
BsoBI CYCGRG 1 cut(s) 1871
Bsp119I TTCGAA 1 cut(s) 1393
Bsp1286I GDGCHC 6 cut(s) 180, 556, 805, 897, 1753, 1937
Bsp143I GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
BspACI CCGC 5 cut(s) 262, 1273, 1291, 1861, 1881
BspANI GGCC 1 cut(s) 1803
BspCNI CTCAG 3 cut(s) 235, 1336, 1915
BspHI TCATGA 2 cut(s) 969, 1560
BspLI GGNNCC 3 cut(s) 81, 913, 1878
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 1 cut(s) 1429
BspT104I TTCGAA 1 cut(s) 1393
BsrDI GCAATG 2 cut(s) 713, 1541
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 4 cut(s) 566, 833, 1038, 1438
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 2 cut(s) 640, 1872
BssMI GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
BssNAI GTATAC 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 640
Bst1107I GTATAC 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 1800, 1805
Bst4CI ACNGT 5 cut(s) 58, 780, 1373, 1748, 1901
Bst6I CTCTTC 2 cut(s) 1770, 1946
BstAPI GCANNNNNTGC 1 cut(s) 1919
BstBAI YACGTR 1 cut(s) 549
BstBI TTCGAA 1 cut(s) 1393
BstC8I GCNNGC 3 cut(s) 207, 897, 1489
BstDEI CTNAG 4 cut(s) 243, 808, 1323, 1923
BstF5I GGATG 2 cut(s) 196, 282
BstKTI GATC 5 cut(s) 621, 1143, 1437, 1560, 1845
BstMAI GTCTC 2 cut(s) 215, 1326
BstMBI GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
BstMWI GCNNNNNNNGC 4 cut(s) 620, 691, 809, 1919
BstNI CCWGG 2 cut(s) 1800, 1805
BstNSI RCATGY 3 cut(s) 700, 1832, 1933
BstSCI CCNGG 2 cut(s) 1798, 1803
BstSFI CTRYAG 2 cut(s) 237, 367
BstSLI GKGCMC 4 cut(s) 180, 556, 805, 1753
BstV1I GCAGC 7 cut(s) 221, 223, 251, 334, 350, 661, 1795
BstV2I GAAGAC 4 cut(s) 31, 331, 1515, 1669
BstX2I RGATCY 2 cut(s) 1140, 1434
BstYI RGATCY 2 cut(s) 1140, 1434
BstZ17I GTATAC 1 cut(s) 651
BsuRI GGCC 1 cut(s) 1803
BtsCI GGATG 2 cut(s) 196, 282
BtsI GCAGTG 1 cut(s) 1751
BtsIMutI CAGTG 3 cut(s) 559, 1617, 1751
Cac8I GCNNGC 3 cut(s) 207, 897, 1489
CciI TCATGA 2 cut(s) 969, 1560
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 912
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 1369, 1465
CspCI CAANNNNNGTGG 2 cut(s) 1672, 1707
CviQI GTAC 2 cut(s) 1369, 1465
DdeI CTNAG 4 cut(s) 243, 808, 1323, 1923
DpnI GATC 5 cut(s) 620, 1142, 1436, 1559, 1844
DpnII GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
DrdI GACNNNNNNGTC 1 cut(s) 1202
DseDI GACNNNNNNGTC 1 cut(s) 1202
Eam1104I CTCTTC 2 cut(s) 1770, 1946
EarI CTCTTC 2 cut(s) 1770, 1946
Ecl136II GAGCTC 1 cut(s) 895
Eco130I CCWWGG 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 897
Eco47I GGWCC 1 cut(s) 912
Eco53kI GAGCTC 1 cut(s) 895
Eco57I CTGAAG 5 cut(s) 325, 452, 985, 1001, 1490
Eco72I CACGTG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 1871
EcoICRI GAGCTC 1 cut(s) 895
EcoRII CCWGG 2 cut(s) 1798, 1803
EcoT14I CCWWGG 1 cut(s) 640
EcoT22I ATGCAT 2 cut(s) 604, 752
EcoT38I GRGCYC 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 215
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 2 cut(s) 581, 925
FauI CCCGC 2 cut(s) 1868, 1888
FauNDI CATATG 1 cut(s) 1039
FbaI TGATCA 2 cut(s) 1557, 1842
FblI GTMKAC 2 cut(s) 650, 1206
Fnu4HI GCNGC 7 cut(s) 210, 237, 240, 323, 364, 675, 1809
FokI GGATG 2 cut(s) 203, 289
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 7 cut(s) 210, 237, 240, 323, 364, 675, 1809
FspBI CTAG 1 cut(s) 725
GluI GCNGC 7 cut(s) 210, 237, 240, 323, 364, 675, 1809
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 2 cut(s) 1553, 1983
HaeIII GGCC 1 cut(s) 1803
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 232
HincII GTYRAC 1 cut(s) 1089
HindII GTYRAC 1 cut(s) 1089
HindIII AAGCTT 1 cut(s) 1643
HinfI GANTC 5 cut(s) 479, 966, 975, 1064, 1303
HpaII CCGG 1 cut(s) 403
HphI GGTGA 3 cut(s) 16, 873, 1994
Hpy166II GTNNAC 5 cut(s) 178, 651, 1089, 1207, 1751
Hpy188I TCNGA 6 cut(s) 84, 118, 471, 484, 980, 1069
Hpy8I GTNNAC 5 cut(s) 178, 651, 1089, 1207, 1751
HpyAV CCTTC 4 cut(s) 551, 1025, 1072, 1485
HpyCH4III ACNGT 5 cut(s) 58, 780, 1373, 1748, 1901
HpyCH4IV ACGT 4 cut(s) 36, 548, 790, 1408
HpyF10VI GCNNNNNNNGC 4 cut(s) 620, 691, 809, 1919
HpyF3I CTNAG 4 cut(s) 243, 808, 1323, 1923
HpySE526I ACGT 4 cut(s) 36, 548, 790, 1408
Ksp22I TGATCA 2 cut(s) 1557, 1842
Kzo9I GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
LmnI GCTCC 3 cut(s) 543, 1694, 1964
Lsp1109I GCAGC 7 cut(s) 221, 223, 251, 334, 350, 661, 1795
LweI GCATC 3 cut(s) 298, 976, 1624
MaeI CTAG 1 cut(s) 725
MaeII ACGT 4 cut(s) 36, 548, 790, 1408
MaeIII GTNAC 4 cut(s) 412, 720, 1020, 1995
MalI GATC 5 cut(s) 620, 1142, 1436, 1559, 1844
MboI GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
MfeI CAATTG 1 cut(s) 1689
MflI RGATCY 2 cut(s) 1140, 1434
MhlI GDGCHC 6 cut(s) 180, 556, 805, 897, 1753, 1937
MluCI AATT 8 cut(s) 151, 287, 387, 437, 948, 1623, 1689, 1847
MlyI GAGTC 2 cut(s) 488, 960
MmeI TCCRAC 3 cut(s) 456, 1311, 1323
Mph1103I ATGCAT 2 cut(s) 604, 752
MroXI GAANNNNTTC 2 cut(s) 38, 504
MseI TTAA 7 cut(s) 255, 377, 393, 419, 1149, 1158, 1989
MslI CAYNNNNRTG 4 cut(s) 695, 1050, 1496, 1727
MspA1I CMGCKG 2 cut(s) 242, 674
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 1800, 1805
MunI CAATTG 1 cut(s) 1689
Mva1269I GAATGC 1 cut(s) 1577
MvaI CCWGG 2 cut(s) 1800, 1805
MwoI GCNNNNNNNGC 4 cut(s) 620, 691, 809, 1919
NdeI CATATG 1 cut(s) 1039
NdeII GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
NlaIV GGNNCC 3 cut(s) 81, 913, 1878
NmuCI GTSAC 1 cut(s) 1995
NsiI ATGCAT 2 cut(s) 604, 752
NspI RCATGY 3 cut(s) 700, 1832, 1933
NspV TTCGAA 1 cut(s) 1393
PagI TCATGA 2 cut(s) 969, 1560
PciI ACATGT 3 cut(s) 696, 1828, 1929
PctI GAATGC 1 cut(s) 1577
PdmI GAANNNNTTC 2 cut(s) 38, 504
PfeI GAWTC 3 cut(s) 975, 1064, 1303
PkrI GCNGC 7 cut(s) 211, 238, 241, 324, 365, 676, 1810
PleI GAGTC 2 cut(s) 487, 960
PmaCI CACGTG 1 cut(s) 549
PmlI CACGTG 1 cut(s) 549
PpsI GAGTC 2 cut(s) 487, 960
Ppu21I YACGTR 1 cut(s) 549
PscI ACATGT 3 cut(s) 696, 1828, 1929
PsiI TTATAA 1 cut(s) 1377
Psp124BI GAGCTC 1 cut(s) 897
Psp6I CCWGG 2 cut(s) 1798, 1803
PspCI CACGTG 1 cut(s) 549
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 2 cut(s) 1798, 1803
PspN4I GGNNCC 3 cut(s) 81, 913, 1878
PspPI GGNCC 1 cut(s) 912
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 1140, 1434
PvuII CAGCTG 2 cut(s) 242, 674
RsaI GTAC 2 cut(s) 1370, 1466
RsaNI GTAC 2 cut(s) 1369, 1465
RseI CAYNNNNRTG 4 cut(s) 695, 1050, 1496, 1727
SacI GAGCTC 1 cut(s) 897
SaqAI TTAA 7 cut(s) 255, 377, 393, 419, 1149, 1158, 1989
SatI GCNGC 7 cut(s) 210, 237, 240, 323, 364, 675, 1809
Sau3AI GATC 5 cut(s) 618, 1140, 1434, 1557, 1842
Sau96I GGNCC 1 cut(s) 912
SchI GAGTC 2 cut(s) 488, 960
ScrFI CCNGG 2 cut(s) 1800, 1805
SduI GDGCHC 6 cut(s) 180, 556, 805, 897, 1753, 1937
SfaNI GCATC 3 cut(s) 298, 976, 1624
SfcI CTRYAG 2 cut(s) 237, 367
SfuI TTCGAA 1 cut(s) 1393
SinI GGWCC 1 cut(s) 912
SmiMI CAYNNNNRTG 4 cut(s) 695, 1050, 1496, 1727
SpeI ACTAGT 1 cut(s) 724
Sse9I AATT 8 cut(s) 151, 287, 387, 437, 948, 1623, 1689, 1847
SsiI CCGC 5 cut(s) 262, 1273, 1291, 1861, 1881
SspI AATATT 2 cut(s) 756, 1815
SspMI CTAG 1 cut(s) 725
SstI GAGCTC 1 cut(s) 897
StyD4I CCNGG 2 cut(s) 1798, 1803
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 5 cut(s) 58, 780, 1373, 1748, 1901
TaiI ACGT 4 cut(s) 39, 551, 793, 1411
TaqI TCGA 4 cut(s) 42, 63, 1393, 1675
TaqII GACCGA 2 cut(s) 325, 929
TasI AATT 8 cut(s) 151, 287, 387, 437, 948, 1623, 1689, 1847
TatI WGTACW 1 cut(s) 1368
TfiI GAWTC 3 cut(s) 975, 1064, 1303
Tru1I TTAA 7 cut(s) 255, 377, 393, 419, 1149, 1158, 1989
Tru9I TTAA 7 cut(s) 255, 377, 393, 419, 1149, 1158, 1989
TscAI CASTG 3 cut(s) 566, 1624, 1758
TseFI GTSAC 1 cut(s) 1995
TseI GCWGC 7 cut(s) 209, 236, 239, 322, 363, 674, 1808
Tsp45I GTSAC 1 cut(s) 1995
TspGWI ACGGA 1 cut(s) 1135
TspRI CASTG 3 cut(s) 566, 1624, 1758
VneI GTGCAC 2 cut(s) 176, 1749
VpaK11BI GGWCC 1 cut(s) 912
XapI RAATTY 3 cut(s) 151, 437, 1847
XceI RCATGY 3 cut(s) 700, 1832, 1933
XmiI GTMKAC 2 cut(s) 650, 1206
XmnI GAANNNNTTC 2 cut(s) 38, 504
XspI CTAG 1 cut(s) 725
Zsp2I ATGCAT 2 cut(s) 604, 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.