Rroxscaffold_2G00102810

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006221
Physical Location & Seq
Reverse (-)
25080321 .. 25086459
6139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00102810.1

Sequence Viewer

Length: 1887 bp
ATGAAAGGCACCACCTTTGGTCTCTTTGAGGTGTTGGGTGGGCTTGAAATGAATGTTGTTGAGGAGGAACGAATTGCTTTTGAGATTGGGGTTGTGGTTCCAAAGAGAAATGTCAAGGCAGAAGAAGAAGATTACTGTGTGGAGGTTTTGGTTAATGAATTTAAGAATGTCGGTTTGGTAGTCGAGAGAGTCCCTGGCGTTGTCGATGACTTTATCAAGCTGGCAGCGCCTTTAGAGACATTGGGGAGGGCTGCGGTTGAGTTGCAAATTAAAAAACGAACTCAAATTGGGATGGATTTACAATTTGAATGGGAGGAGGTTAATGCTTTTGTGAGGCAGCCTGATGGTTCATTGTTCAGCTGGTGTGAGCGTTTTCATTGCTATAGGCACTTGATATATGGAATTATGAACAAGAGCAAGTCAGCTGTAACTCTGAGATTTGATGGGAAAGAGTTCTGTTGGGAAGTTGGGGAATGTTTGCTTAAGAAATTGGAGTCACAGAGGATTGTCAAAGAAGTTTTTCCTTTGCATGATGAAGTGAAAAGGAAGAAACTCCTTCACACTTGGGCTCTGCACTGGTGGGACTTCACAAGCCAGCCCATAGATGAGATTTACTCATATTTTGGTACAAAGTTCTGGAAACGCAAAAATGTTGCTCTGTTAACTAGATGGCAGATCAGTTATCCAATTAGAACCGACCTGGGAAATAGACAGCTGGGCATGGCTTGGAGTTCCTCGCAGTCATCTGTAGAACTCATGAAGAAATGGGGGACAGACAAAACAAAAGAGAAAGAAATGTTTCAAAGAATTGAGTGGTTTGGACGACTATTGAGATTCCGAAATGATGCTATCATTATAGTTAGTATTATATGCCTCCAGTTACCGTTTGAGCTGGCATATGCTCATCTTTACGAGGTTATTAAGTCTGATATTGTGAAGTTTGGGTTGACAACCATATATCTTTTTGCAATTCAGTTTTTTACGAAGCTAGGGGGCAAGATCTCTGTTAAGCTTATTAAATACGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGTTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATACATAGGAGTCTTCTACCATGCCCTTATACACCGGAACTTTTTGACTCTTCGTACAGTCTTGATTCAGCGCCTCCTTGTGTCTGAGGTATTGGAAAACTTGGTGGAAAACACTTTACCATATTTGAAGTACAGTTATAAGAAGTTCAGAGTTCGAAGCAAGAAGAAACGCGAAAGAGGATCATCAACAGGGAAGATCCATTTTGCTTCTAGGGTGGAAAAGGAGTACTTGAAGCCTTCTTATTCTGCAAGCATTGGTGTGGAACTTGAAGATGGGCTTTTCGATGACTTTCTAGAGTTAGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCCTTCCCCCTGCTTTTGCCTTTGCGGCTGTGGTATTGTGCTTCTCTTTGTACAAACAACATAACTGAAATTAGGACAGATGCATTGAAGCTGCTTGTGATGCATAAGAGGCCAATTCCTCGTGTTTCCACAACAATTGGTGCTTGGCTGAACATATTTCAGTTTCTTGTTGTTACATCCATATGCACCAACTGCGCCCTTCTAGTATGGTTATATGATCATGAGGGGAAATGGAATATAGAGCCTGGCCTAGCTGCAATTCTGGTTATGGAACATATCCTCCTGTTGATTAAGTTTGGTTTCTCTCGCCTTGTACCCGAGGAACCTGCTTGGGTACGGGCGAACCGTGTGAAAAGAGCAACAGAGGCCGAGTACATGTGCTCCAAACAGCTTTTGAGGAATATTTCTGGAGATAGGGCAGCTGGTACTAGTGCAGTTAAGAAGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

628

Amino Acids

73.0

Weight (kDa)

9.03

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 206 - 596 4.5e-73 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1236
AasI GACNNNNNNGTC 1 cut(s) 1061
Acc36I ACCTGC 1 cut(s) 1768
AccB1I GGYRCC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 1 cut(s) 1269
AciI CCGC 2 cut(s) 254, 1459
AclWI GGATC 2 cut(s) 1285, 1288
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 9 cut(s) 628, 1153, 1229, 1325, 1486, 1749, 1770, 1808, 1861
AflII CTTAAG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 1809
AgsI TTSAA 7 cut(s) 47, 308, 803, 1225, 1330, 1367, 1522
AhlI ACTAGT 1 cut(s) 1862
AjnI CCWGG 3 cut(s) 193, 699, 1678
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AloI GAACNNNNNNTCC 2 cut(s) 1698, 1730
Alw21I GWGCWC 1 cut(s) 1817
Alw26I GTCTC 2 cut(s) 26, 230
AlwI GGATC 2 cut(s) 1285, 1288
Ama87I CYCGRG 1 cut(s) 1751
AoxI GGCC 3 cut(s) 1544, 1681, 1800
ApeKI GCWGC 6 cut(s) 224, 251, 337, 1525, 1688, 1853
ApoI RAATTY 1 cut(s) 158
Asp700I GAANNNNTTC 3 cut(s) 452, 519, 798
AspLEI GCGC 3 cut(s) 229, 1170, 1631
AsuII TTCGAA 1 cut(s) 1252
AvaI CYCGRG 1 cut(s) 1751
BanI GGYRCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 1554
BbsI GAAGAC 1 cut(s) 1102
Bbv12I GWGCWC 1 cut(s) 1817
BbvI GCAGC 6 cut(s) 236, 238, 349, 1512, 1675, 1865
BccI CCATC 5 cut(s) 286, 338, 437, 663, 1364
BcgI CGANNNNNNTGC 2 cut(s) 1742, 1776
BciT130I CCWGG 3 cut(s) 195, 701, 1680
BclI TGATCA 2 cut(s) 1416, 1651
BcoDI GTCTC 2 cut(s) 26, 230
BcuI ACTAGT 1 cut(s) 1862
BfaI CTAG 7 cut(s) 666, 989, 1308, 1391, 1637, 1685, 1863
BfmI CTRYAG 2 cut(s) 382, 747
BfoI RGCGCY 2 cut(s) 230, 1171
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 1768
BglI GCCNNNNNGGC 1 cut(s) 1459
BglII AGATCT 1 cut(s) 999
BisI GCNGC 7 cut(s) 225, 252, 338, 1460, 1526, 1689, 1854
BlsI GCNGC 7 cut(s) 226, 253, 339, 1461, 1527, 1690, 1855
BmcAI AGTACT 1 cut(s) 1325
Bme1390I CCNGG 3 cut(s) 195, 701, 1680
BmeT110I CYCGRG 1 cut(s) 1751
BmiI GGNNCC 3 cut(s) 10, 99, 1758
BmrFI CCNGG 3 cut(s) 195, 701, 1680
BmsI GCATC 3 cut(s) 835, 1504, 1524
BpiI GAAGAC 1 cut(s) 1102
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 2 cut(s) 860, 1863
Bpu14I TTCGAA 1 cut(s) 1252
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 3 cut(s) 193, 700, 1752
BsaWI WCCGGW 1 cut(s) 1131
BsaXI ACNNNNNCTCC 4 cut(s) 1698, 1728, 1799, 1829
Bse1I ACTGG 2 cut(s) 581, 877
Bse3DI GCAATG 2 cut(s) 376, 1400
BseBI CCWGG 3 cut(s) 195, 701, 1680
BseDI CCNNGG 3 cut(s) 193, 700, 1752
BseGI GGATG 2 cut(s) 297, 1610
BseMI GCAATG 2 cut(s) 376, 1400
BseMII CTCAG 2 cut(s) 425, 1173
BseNI ACTGG 2 cut(s) 581, 877
BseRI GAGGAG 2 cut(s) 77, 329
BseXI GCAGC 6 cut(s) 236, 238, 349, 1512, 1675, 1865
BseYI CCCAGC 1 cut(s) 715
BsgI GTGCAG 2 cut(s) 557, 1887
Bsh1236I CGCG 1 cut(s) 1269
BshFI GGCC 3 cut(s) 1546, 1683, 1802
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 1817
BsiHKCI CYCGRG 1 cut(s) 1751
BsiSI CCGG 1 cut(s) 1132
BslFI GGGAC 3 cut(s) 176, 596, 784
BsmAI GTCTC 2 cut(s) 26, 230
BsmFI GGGAC 3 cut(s) 176, 596, 784
BsnI GGCC 3 cut(s) 1546, 1683, 1802
Bso31I GGTCTC 1 cut(s) 26
BsoBI CYCGRG 1 cut(s) 1751
Bsp119I TTCGAA 1 cut(s) 1252
Bsp1286I GDGCHC 2 cut(s) 571, 1817
Bsp1407I TGTACA 1 cut(s) 1484
Bsp143I GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
BspACI CCGC 2 cut(s) 254, 1459
BspANI GGCC 3 cut(s) 1546, 1683, 1802
BspCNI CTCAG 2 cut(s) 426, 1174
BspFNI CGCG 1 cut(s) 1269
BspHI TCATGA 3 cut(s) 756, 1419, 1654
BspLI GGNNCC 3 cut(s) 10, 99, 1758
BspMI ACCTGC 1 cut(s) 1768
BspPI GGATC 2 cut(s) 1285, 1288
BspT104I TTCGAA 1 cut(s) 1252
BspT107I GGYRCC 1 cut(s) 8
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 2 cut(s) 376, 1400
BsrGI TGTACA 1 cut(s) 1484
BsrI ACTGG 2 cut(s) 581, 877
BssECI CCNNGG 3 cut(s) 193, 700, 1752
BssMI GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
BssSI CACGAG 1 cut(s) 1554
Bst2BI CACGAG 1 cut(s) 1554
Bst2UI CCWGG 3 cut(s) 195, 701, 1680
Bst4CI ACNGT 6 cut(s) 137, 885, 1058, 1156, 1232, 1781
Bst6I CTCTTC 1 cut(s) 1152
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1626
BstAUI TGTACA 1 cut(s) 1484
BstBI TTCGAA 1 cut(s) 1252
BstC8I GCNNGC 4 cut(s) 222, 596, 894, 1348
BstDEI CTNAG 2 cut(s) 434, 1182
BstF5I GGATG 2 cut(s) 297, 1610
BstFNI CGCG 1 cut(s) 1269
BstH2I RGCGCY 2 cut(s) 230, 1171
BstHHI GCGC 3 cut(s) 229, 1170, 1631
BstKTI GATC 6 cut(s) 678, 1002, 1280, 1296, 1419, 1654
BstMAI GTCTC 2 cut(s) 26, 230
BstMBI GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
BstMWI GCNNNNNNNGC 6 cut(s) 226, 1459, 1534, 1543, 1626, 1799
BstNI CCWGG 3 cut(s) 195, 701, 1680
BstNSI RCATGY 1 cut(s) 1813
BstSCI CCNGG 3 cut(s) 193, 699, 1678
BstSFI CTRYAG 2 cut(s) 382, 747
BstUI CGCG 1 cut(s) 1269
BstV1I GCAGC 6 cut(s) 236, 238, 349, 1512, 1675, 1865
BstV2I GAAGAC 1 cut(s) 1102
BstX2I RGATCY 2 cut(s) 999, 1293
BstYI RGATCY 2 cut(s) 999, 1293
BsuRI GGCC 3 cut(s) 1546, 1683, 1802
BtsCI GGATG 2 cut(s) 297, 1610
BtsIMutI CAGTG 1 cut(s) 574
BveI ACCTGC 1 cut(s) 1768
Cac8I GCNNGC 4 cut(s) 222, 596, 894, 1348
CciI TCATGA 3 cut(s) 756, 1419, 1654
CfoI GCGC 3 cut(s) 229, 1170, 1631
Csp6I GTAC 9 cut(s) 627, 1152, 1228, 1324, 1485, 1748, 1769, 1807, 1860
CspCI CAANNNNNGTGG 2 cut(s) 1552, 1587
CviAII CATG 8 cut(s) 530, 721, 757, 1090, 1118, 1420, 1655, 1810
CviQI GTAC 9 cut(s) 627, 1152, 1228, 1324, 1485, 1748, 1769, 1807, 1860
DdeI CTNAG 2 cut(s) 434, 1182
DpnI GATC 6 cut(s) 677, 1001, 1279, 1295, 1418, 1653
DpnII GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
DrdI GACNNNNNNGTC 1 cut(s) 1061
DseDI GACNNNNNNGTC 1 cut(s) 1061
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 1751
EcoRII CCWGG 3 cut(s) 193, 699, 1678
EcoT22I ATGCAT 2 cut(s) 1519, 1539
EcoT38I GRGCYC 1 cut(s) 571
FaeI CATG 8 cut(s) 533, 724, 760, 1093, 1121, 1423, 1658, 1813
FalI AAGNNNNNCTT 4 cut(s) 1310, 1342, 1359, 1391
FaqI GGGAC 3 cut(s) 176, 596, 784
FatI CATG 8 cut(s) 529, 720, 756, 1089, 1117, 1419, 1654, 1809
FauNDI CATATG 2 cut(s) 898, 1616
FbaI TGATCA 2 cut(s) 1416, 1651
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 7 cut(s) 225, 252, 338, 1460, 1526, 1689, 1854
FokI GGATG 2 cut(s) 304, 1597
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 7 cut(s) 225, 252, 338, 1460, 1526, 1689, 1854
FspBI CTAG 7 cut(s) 666, 989, 1308, 1391, 1637, 1685, 1863
GlaI GCGC 3 cut(s) 228, 1169, 1630
GluI GCNGC 7 cut(s) 225, 252, 338, 1460, 1526, 1689, 1854
GsaI CCCAGC 1 cut(s) 719
GsuI CTGGAG 2 cut(s) 860, 1863
HaeII RGCGCY 2 cut(s) 230, 1171
HaeIII GGCC 3 cut(s) 1546, 1683, 1802
HapII CCGG 1 cut(s) 1132
HhaI GCGC 3 cut(s) 229, 1170, 1631
Hin1II CATG 8 cut(s) 533, 724, 760, 1093, 1121, 1423, 1658, 1813
Hin6I GCGC 3 cut(s) 227, 1168, 1629
HinP1I GCGC 3 cut(s) 227, 1168, 1629
HincII GTYRAC 2 cut(s) 663, 948
HindII GTYRAC 2 cut(s) 663, 948
HindIII AAGCTT 1 cut(s) 1010
HinfI GANTC 6 cut(s) 189, 494, 834, 1107, 1144, 1162
HpaI GTTAAC 1 cut(s) 663
HpaII CCGG 1 cut(s) 1132
Hpy166II GTNNAC 3 cut(s) 663, 948, 1066
Hpy188I TCNGA 5 cut(s) 435, 839, 928, 1183, 1247
Hpy188III TCNNGA 8 cut(s) 184, 637, 757, 1159, 1391, 1420, 1655, 1842
Hpy8I GTNNAC 3 cut(s) 663, 948, 1066
HpyAV CCTTC 5 cut(s) 566, 1344, 1447, 1643, 1870
HpyCH4III ACNGT 6 cut(s) 137, 885, 1058, 1156, 1232, 1781
HpyF10VI GCNNNNNNNGC 6 cut(s) 226, 1459, 1534, 1543, 1626, 1799
HpyF3I CTNAG 2 cut(s) 434, 1182
Hsp92II CATG 8 cut(s) 533, 724, 760, 1093, 1121, 1423, 1658, 1813
HspAI GCGC 3 cut(s) 227, 1168, 1629
Ksp22I TGATCA 2 cut(s) 1416, 1651
KspAI GTTAAC 1 cut(s) 663
Kzo9I GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
LmnI GCTCC 1 cut(s) 1820
Lsp1109I GCAGC 6 cut(s) 236, 238, 349, 1512, 1675, 1865
LweI GCATC 3 cut(s) 835, 1504, 1524
MaeI CTAG 7 cut(s) 666, 989, 1308, 1391, 1637, 1685, 1863
MaeIII GTNAC 4 cut(s) 427, 495, 879, 1606
MalI GATC 6 cut(s) 677, 1001, 1279, 1295, 1418, 1653
MboI GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
MfeI CAATTG 1 cut(s) 1569
MflI RGATCY 2 cut(s) 999, 1293
MhlI GDGCHC 2 cut(s) 571, 1817
MlyI GAGTC 4 cut(s) 198, 503, 1116, 1138
Mph1103I ATGCAT 2 cut(s) 1519, 1539
MroXI GAANNNNTTC 3 cut(s) 452, 519, 798
MslI CAYNNNNRTG 2 cut(s) 1355, 1615
MspA1I CMGCKG 4 cut(s) 360, 425, 715, 1856
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 1 cut(s) 1132
MspR9I CCNGG 3 cut(s) 195, 701, 1680
MunI CAATTG 1 cut(s) 1569
MvaI CCWGG 3 cut(s) 195, 701, 1680
MvnI CGCG 1 cut(s) 1269
MwoI GCNNNNNNNGC 6 cut(s) 226, 1459, 1534, 1543, 1626, 1799
NdeI CATATG 2 cut(s) 898, 1616
NdeII GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
NlaIII CATG 8 cut(s) 533, 724, 760, 1093, 1121, 1423, 1658, 1813
NlaIV GGNNCC 3 cut(s) 10, 99, 1758
NmeAIII GCCGAG 1 cut(s) 1828
NmuCI GTSAC 1 cut(s) 495
NsiI ATGCAT 2 cut(s) 1519, 1539
NspI RCATGY 1 cut(s) 1813
NspV TTCGAA 1 cut(s) 1252
PagI TCATGA 3 cut(s) 756, 1419, 1654
PciI ACATGT 1 cut(s) 1809
PcsI WCGNNNNNNNCGW 1 cut(s) 1777
PdmI GAANNNNTTC 3 cut(s) 452, 519, 798
PfeI GAWTC 2 cut(s) 834, 1162
PkrI GCNGC 7 cut(s) 226, 253, 339, 1461, 1527, 1690, 1855
PleI GAGTC 4 cut(s) 197, 502, 1115, 1138
PpsI GAGTC 4 cut(s) 197, 502, 1115, 1138
PscI ACATGT 1 cut(s) 1809
PsiI TTATAA 1 cut(s) 1236
Psp6I CCWGG 3 cut(s) 193, 699, 1678
PspFI CCCAGC 1 cut(s) 715
PspGI CCWGG 3 cut(s) 193, 699, 1678
PspN4I GGNNCC 3 cut(s) 10, 99, 1758
PsuI RGATCY 2 cut(s) 999, 1293
PvuII CAGCTG 4 cut(s) 360, 425, 715, 1856
RsaI GTAC 9 cut(s) 628, 1153, 1229, 1325, 1486, 1749, 1770, 1808, 1861
RsaNI GTAC 9 cut(s) 627, 1152, 1228, 1324, 1485, 1748, 1769, 1807, 1860
RseI CAYNNNNRTG 2 cut(s) 1355, 1615
SatI GCNGC 7 cut(s) 225, 252, 338, 1460, 1526, 1689, 1854
Sau3AI GATC 6 cut(s) 675, 999, 1277, 1293, 1416, 1651
ScaI AGTACT 1 cut(s) 1325
SchI GAGTC 4 cut(s) 198, 503, 1116, 1138
ScrFI CCNGG 3 cut(s) 195, 701, 1680
SduI GDGCHC 2 cut(s) 571, 1817
SfaNI GCATC 3 cut(s) 835, 1504, 1524
SfcI CTRYAG 2 cut(s) 382, 747
SfuI TTCGAA 1 cut(s) 1252
SmiMI CAYNNNNRTG 2 cut(s) 1355, 1615
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SpeI ACTAGT 1 cut(s) 1862
SsiI CCGC 2 cut(s) 254, 1459
SspI AATATT 1 cut(s) 1837
SspMI CTAG 7 cut(s) 666, 989, 1308, 1391, 1637, 1685, 1863
StyD4I CCNGG 3 cut(s) 193, 699, 1678
TaaI ACNGT 6 cut(s) 137, 885, 1058, 1156, 1232, 1781
TaqI TCGA 4 cut(s) 183, 204, 1252, 1380
TatI WGTACW 4 cut(s) 1227, 1323, 1484, 1806
TauI GCSGC 1 cut(s) 1462
TfiI GAWTC 2 cut(s) 834, 1162
TscAI CASTG 1 cut(s) 581
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 6 cut(s) 224, 251, 337, 1525, 1688, 1853
Tsp45I GTSAC 1 cut(s) 495
TspRI CASTG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 482
XapI RAATTY 1 cut(s) 158
XbaI TCTAGA 1 cut(s) 1390
XceI RCATGY 1 cut(s) 1813
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 3 cut(s) 452, 519, 798
XspI CTAG 7 cut(s) 666, 989, 1308, 1391, 1637, 1685, 1863
ZrmI AGTACT 1 cut(s) 1325
Zsp2I ATGCAT 2 cut(s) 1519, 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.