Rh2DG443100

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006221
Physical Location & Seq
Forward (+)
64354905 .. 64360795
5891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG443100.1

Sequence Viewer

Length: 1737 bp
ATGGATTTACAATTTGAATGGGAGGAGGTTGATGCTTTTGTGAGGCAGCCTGATGGTTCATTGTTCAGCTGGTGTGAGCGTTTTCATTGCTATAGGCACTTGATATATGGAATTATGAACAAGAGCAAGTCAACTGTAACTCTGAGATTTGATGGGAAAGAGTTCTATTGGGAAGTTGGGGAATGTTTGCTTAAGAAATTGGAGTCACAGAGGATTGTCAAAGAAGTTTTTCCTTTGCATGATGAAGTGAAAAGGAAGAAACTCCTTCACACTTGGGCTCTGCACTGGTGGGACTTCACAAGCCAGCCCATAGATGAGATTTACTCATATTTTGGTACAAAGATTGCCATCTATTTTACTTTTCTTGGAATGTATACACGGTGGTTGCTGTTTCCAGCTACATTTGGACTTATTCTGCAGATATTTGATTTTGGGTCGTTGCAGTTACTGGTGCTCCCCCTTTTCTTTATAAGCATAACCTTATGGGCTATAATGTTTTCCCAGTTCTGGAAACGCAAAAATGTTGCTCTGTTAACTAGATGGCAGATCAGTTATCCAATTAGAACCGACCTGGGAAATAGGCAGCTGGGCATGGCTTGGAGTTCCTCGCAGTCATCTGTAGAACTCATGAAGAAATGGGGGACAGACAAAACAAAAGAGAAAGAAATGTTTCAAAGAATTGAGTGGTTTGGACGACTACTGAGATTCCGAAATGATGCTATCATTATAGTTAGTATTATATGCCTCCAGTTGCCGTTTGAGCTGGCATATGCTCATCTTTATGAGGTTATTAAGTCTGATATTGTGAAGTTTGGGTTGACAACCATATATCTTTTTGCAATTCAGTTTTTTACGAAGCTAGGGGGCAAGATCTCTGTTAAGCTTATTAAACACGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATACATAGGAGTCTTCTACCATGCCCTTATACACCGGAACTTTTTGACTCTTCGTACAGTCTTGATTCAGCGCCTCCTTGTGTCTGAGGTATTGGAAAACCTGGTGGAAAACACTGTACCATATTTGAAGTACAGTTATAAGAAGTTCAGAGTTCGAAGCAAGAAGAAACGCGAAAGAGGATCATCAACAGGGAAGATCCACTTTGCTTCTAGGGTGGAAAAGGAGTACTTGAAGCCTTCTTATTCTGCAAGCATTGGTGTGGAACTTGAAGATGGGCTTTTCGATGACTTTCTAGAGTTAGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCCTTCCCCCCTGCTTTTGCCTTTGCGGCTGTGAACAACATAACTGAAATTAGGACAGATGCATTGAAGCTGCTTGTGATGCATAAGAGGCCAATTCCTCGTGTTTCCACAACAATTGGTGCTTGGCTGAACATATTTCAGTTTCTTGTTGTTACATCCATATGCACCAACTGCGCCCTTCTAGTATGGTTATATGATCATGAGGGGAAATGGAATATAGAGCCTGGCCTAGCTGCAATTCTGGTTATGGAACATATCCTCCTGTTGATTAAGTTTGGTTTCTCTCGCCTTGTACCCGAGGAACCTGCTTGGGTACGGGCGAACCGTGTGAAAAGAGCAACAGAGGCCGAGTACATGTGCTCCAAACAGCTTTTGAGGAATATTTCTGGAGATAGGGCAGCTGGTACTAGTGCAGTTAAGAAGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

578

Amino Acids

67.69

Weight (kDa)

9.3

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 107 - 546 5.8e-100 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 470, 1107
AasI GACNNNNNNGTC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 1618
AccI GTMKAC 2 cut(s) 374, 936
AccII CGCG 1 cut(s) 1140
AciI CCGC 1 cut(s) 1331
AclWI GGATC 2 cut(s) 1156, 1159
AfaI GTAC 9 cut(s) 337, 1024, 1086, 1100, 1196, 1599, 1620, 1658, 1711
AfiI CCNNNNNNNGG 1 cut(s) 507
AflII CTTAAG 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 1659
AgsI TTSAA 6 cut(s) 17, 674, 1096, 1201, 1238, 1372
AhlI ACTAGT 1 cut(s) 1712
AjnI CCWGG 3 cut(s) 570, 1068, 1528
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 2 cut(s) 456, 1667
AlwI GGATC 2 cut(s) 1156, 1159
AlwNI CAGNNNCTG 1 cut(s) 448
Ama87I CYCGRG 1 cut(s) 1601
AoxI GGCC 3 cut(s) 1394, 1531, 1650
ApeKI GCWGC 5 cut(s) 46, 583, 1375, 1538, 1703
Asp700I GAANNNNTTC 3 cut(s) 161, 228, 669
AspLEI GCGC 2 cut(s) 1041, 1481
AsuII TTCGAA 1 cut(s) 1123
AvaI CYCGRG 1 cut(s) 1601
BanII GRGCYC 1 cut(s) 280
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 1 cut(s) 973
Bbv12I GWGCWC 2 cut(s) 456, 1667
BbvI GCAGC 5 cut(s) 58, 595, 1362, 1525, 1715
BccI CCATC 5 cut(s) 47, 146, 356, 534, 1235
BceAI ACGGC 1 cut(s) 739
BcgI CGANNNNNNTGC 2 cut(s) 1592, 1626
BciT130I CCWGG 3 cut(s) 572, 1070, 1530
BclI TGATCA 2 cut(s) 1287, 1501
BcuI ACTAGT 1 cut(s) 1712
BfaI CTAG 7 cut(s) 537, 860, 1179, 1262, 1487, 1535, 1713
BfmI CTRYAG 3 cut(s) 91, 416, 618
BfoI RGCGCY 1 cut(s) 1042
BfrI CTTAAG 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 1618
BglI GCCNNNNNGGC 1 cut(s) 1331
BglII AGATCT 1 cut(s) 870
BisI GCNGC 6 cut(s) 47, 584, 1332, 1376, 1539, 1704
BlsI GCNGC 6 cut(s) 48, 585, 1333, 1377, 1540, 1705
BmcAI AGTACT 1 cut(s) 1196
Bme1390I CCNGG 3 cut(s) 572, 1070, 1530
BmeT110I CYCGRG 1 cut(s) 1601
BmiI GGNNCC 1 cut(s) 1608
BmrFI CCNGG 3 cut(s) 572, 1070, 1530
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 4 cut(s) 22, 706, 1354, 1374
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 973
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 2 cut(s) 731, 1713
Bpu14I TTCGAA 1 cut(s) 1123
BsaBI GATNNNNATC 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 571, 1602
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 6 cut(s) 438, 468, 1548, 1578, 1649, 1679
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 4 cut(s) 290, 453, 502, 748
Bse3DI GCAATG 2 cut(s) 85, 1271
Bse8I GATNNNNATC 1 cut(s) 347
BseBI CCWGG 3 cut(s) 572, 1070, 1530
BseDI CCNNGG 2 cut(s) 571, 1602
BseGI GGATG 1 cut(s) 1460
BseJI GATNNNNATC 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMI GCAATG 2 cut(s) 85, 1271
BseMII CTCAG 3 cut(s) 134, 692, 1044
BseNI ACTGG 4 cut(s) 290, 453, 502, 748
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 5 cut(s) 58, 595, 1362, 1525, 1715
BseYI CCCAGC 1 cut(s) 586
BsgI GTGCAG 2 cut(s) 266, 1737
Bsh1236I CGCG 1 cut(s) 1140
BshFI GGCC 3 cut(s) 1396, 1533, 1652
BsiHKAI GWGCWC 2 cut(s) 456, 1667
BsiHKCI CYCGRG 1 cut(s) 1601
BsiSI CCGG 1 cut(s) 1003
BslFI GGGAC 2 cut(s) 305, 655
BslI CCNNNNNNNGG 1 cut(s) 507
BsmFI GGGAC 2 cut(s) 305, 655
BsnI GGCC 3 cut(s) 1396, 1533, 1652
BsoBI CYCGRG 1 cut(s) 1601
Bsp119I TTCGAA 1 cut(s) 1123
Bsp1286I GDGCHC 3 cut(s) 280, 456, 1667
Bsp143I GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
BspACI CCGC 1 cut(s) 1331
BspANI GGCC 3 cut(s) 1396, 1533, 1652
BspCNI CTCAG 3 cut(s) 135, 693, 1045
BspFNI CGCG 1 cut(s) 1140
BspHI TCATGA 3 cut(s) 627, 1290, 1504
BspLI GGNNCC 1 cut(s) 1608
BspMAI CTGCAG 1 cut(s) 420
BspMI ACCTGC 1 cut(s) 1618
BspPI GGATC 2 cut(s) 1156, 1159
BspT104I TTCGAA 1 cut(s) 1123
BspTI CTTAAG 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 85, 1271
BsrI ACTGG 4 cut(s) 290, 453, 502, 748
BssECI CCNNGG 2 cut(s) 571, 1602
BssMI GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
BssNAI GTATAC 1 cut(s) 375
BssSI CACGAG 1 cut(s) 1404
Bst1107I GTATAC 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 3 cut(s) 572, 1070, 1530
Bst4CI ACNGT 6 cut(s) 136, 381, 1027, 1084, 1103, 1631
Bst6I CTCTTC 1 cut(s) 1023
BstAFI CTTAAG 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 1476
BstBI TTCGAA 1 cut(s) 1123
BstC8I GCNNGC 3 cut(s) 305, 765, 1219
BstDEI CTNAG 3 cut(s) 143, 701, 1053
BstF5I GGATG 1 cut(s) 1460
BstFNI CGCG 1 cut(s) 1140
BstH2I RGCGCY 1 cut(s) 1042
BstHHI GCGC 2 cut(s) 1041, 1481
BstKTI GATC 6 cut(s) 549, 873, 1151, 1167, 1290, 1504
BstMBI GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
BstMWI GCNNNNNNNGC 6 cut(s) 760, 1331, 1384, 1393, 1476, 1649
BstNI CCWGG 3 cut(s) 572, 1070, 1530
BstNSI RCATGY 1 cut(s) 1663
BstSCI CCNGG 3 cut(s) 570, 1068, 1528
BstSFI CTRYAG 3 cut(s) 91, 416, 618
BstUI CGCG 1 cut(s) 1140
BstV1I GCAGC 5 cut(s) 58, 595, 1362, 1525, 1715
BstV2I GAAGAC 1 cut(s) 973
BstX2I RGATCY 2 cut(s) 870, 1164
BstYI RGATCY 2 cut(s) 870, 1164
BstZ17I GTATAC 1 cut(s) 375
BsuRI GGCC 3 cut(s) 1396, 1533, 1652
BtsCI GGATG 1 cut(s) 1460
BtsIMutI CAGTG 2 cut(s) 283, 1080
BveI ACCTGC 1 cut(s) 1618
Cac8I GCNNGC 3 cut(s) 305, 765, 1219
CaiI CAGNNNCTG 1 cut(s) 448
CciI TCATGA 3 cut(s) 627, 1290, 1504
CfoI GCGC 2 cut(s) 1041, 1481
CsiI ACCWGGT 1 cut(s) 1068
Csp6I GTAC 9 cut(s) 336, 1023, 1085, 1099, 1195, 1598, 1619, 1657, 1710
CspCI CAANNNNNGTGG 2 cut(s) 1402, 1437
CviAII CATG 8 cut(s) 239, 592, 628, 961, 989, 1291, 1505, 1660
CviQI GTAC 9 cut(s) 336, 1023, 1085, 1099, 1195, 1598, 1619, 1657, 1710
DdeI CTNAG 3 cut(s) 143, 701, 1053
DpnI GATC 6 cut(s) 548, 872, 1150, 1166, 1289, 1503
DpnII GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
DrdI GACNNNNNNGTC 1 cut(s) 932
DseDI GACNNNNNNGTC 1 cut(s) 932
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
Eco24I GRGCYC 1 cut(s) 280
Eco88I CYCGRG 1 cut(s) 1601
EcoRII CCWGG 3 cut(s) 570, 1068, 1528
EcoT22I ATGCAT 2 cut(s) 1369, 1389
EcoT38I GRGCYC 1 cut(s) 280
FaeI CATG 8 cut(s) 242, 595, 631, 964, 992, 1294, 1508, 1663
FalI AAGNNNNNCTT 6 cut(s) 1154, 1186, 1181, 1213, 1230, 1262
FaqI GGGAC 2 cut(s) 305, 655
FatI CATG 8 cut(s) 238, 591, 627, 960, 988, 1290, 1504, 1659
FauNDI CATATG 2 cut(s) 769, 1466
FbaI TGATCA 2 cut(s) 1287, 1501
FblI GTMKAC 2 cut(s) 374, 936
Fnu4HI GCNGC 6 cut(s) 47, 584, 1332, 1376, 1539, 1704
FokI GGATG 1 cut(s) 1447
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 6 cut(s) 47, 584, 1332, 1376, 1539, 1704
FspBI CTAG 7 cut(s) 537, 860, 1179, 1262, 1487, 1535, 1713
GlaI GCGC 2 cut(s) 1040, 1480
GluI GCNGC 6 cut(s) 47, 584, 1332, 1376, 1539, 1704
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 2 cut(s) 731, 1713
HaeII RGCGCY 1 cut(s) 1042
HaeIII GGCC 3 cut(s) 1396, 1533, 1652
HapII CCGG 1 cut(s) 1003
HhaI GCGC 2 cut(s) 1041, 1481
Hin1II CATG 8 cut(s) 242, 595, 631, 964, 992, 1294, 1508, 1663
Hin6I GCGC 2 cut(s) 1039, 1479
HinP1I GCGC 2 cut(s) 1039, 1479
HincII GTYRAC 3 cut(s) 132, 534, 819
HindII GTYRAC 3 cut(s) 132, 534, 819
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 5 cut(s) 203, 705, 978, 1015, 1033
HpaI GTTAAC 1 cut(s) 534
HpaII CCGG 1 cut(s) 1003
Hpy166II GTNNAC 6 cut(s) 132, 375, 534, 819, 937, 1339
Hpy188I TCNGA 5 cut(s) 144, 710, 799, 1054, 1118
Hpy188III TCNNGA 7 cut(s) 508, 628, 1030, 1262, 1291, 1505, 1692
Hpy8I GTNNAC 6 cut(s) 132, 375, 534, 819, 937, 1339
HpyAV CCTTC 5 cut(s) 275, 1215, 1318, 1493, 1720
HpyCH4III ACNGT 6 cut(s) 136, 381, 1027, 1084, 1103, 1631
HpyF10VI GCNNNNNNNGC 6 cut(s) 760, 1331, 1384, 1393, 1476, 1649
HpyF3I CTNAG 3 cut(s) 143, 701, 1053
Hsp92II CATG 8 cut(s) 242, 595, 631, 964, 992, 1294, 1508, 1663
HspAI GCGC 2 cut(s) 1039, 1479
Ksp22I TGATCA 2 cut(s) 1287, 1501
KspAI GTTAAC 1 cut(s) 534
Kzo9I GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
LmnI GCTCC 2 cut(s) 459, 1670
Lsp1109I GCAGC 5 cut(s) 58, 595, 1362, 1525, 1715
LweI GCATC 4 cut(s) 22, 706, 1354, 1374
MabI ACCWGGT 1 cut(s) 1068
MaeI CTAG 7 cut(s) 537, 860, 1179, 1262, 1487, 1535, 1713
MaeIII GTNAC 4 cut(s) 136, 204, 444, 1456
MalI GATC 6 cut(s) 548, 872, 1150, 1166, 1289, 1503
MboI GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
MboII GAAGA 7 cut(s) 268, 643, 973, 1010, 1144, 1174, 1250
MfeI CAATTG 1 cut(s) 1419
MflI RGATCY 2 cut(s) 870, 1164
MhlI GDGCHC 3 cut(s) 280, 456, 1667
MlyI GAGTC 3 cut(s) 212, 987, 1009
Mph1103I ATGCAT 2 cut(s) 1369, 1389
MroXI GAANNNNTTC 3 cut(s) 161, 228, 669
MseI TTAA 7 cut(s) 192, 533, 792, 879, 888, 1575, 1722
MslI CAYNNNNRTG 3 cut(s) 780, 1226, 1465
MspA1I CMGCKG 3 cut(s) 69, 586, 1706
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 3 cut(s) 572, 1070, 1530
MunI CAATTG 1 cut(s) 1419
MvaI CCWGG 3 cut(s) 572, 1070, 1530
MvnI CGCG 1 cut(s) 1140
MwoI GCNNNNNNNGC 6 cut(s) 760, 1331, 1384, 1393, 1476, 1649
NdeI CATATG 2 cut(s) 769, 1466
NdeII GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
NlaIII CATG 8 cut(s) 242, 595, 631, 964, 992, 1294, 1508, 1663
NlaIV GGNNCC 1 cut(s) 1608
NmeAIII GCCGAG 1 cut(s) 1678
NmuCI GTSAC 1 cut(s) 204
NsiI ATGCAT 2 cut(s) 1369, 1389
NspI RCATGY 1 cut(s) 1663
NspV TTCGAA 1 cut(s) 1123
PagI TCATGA 3 cut(s) 627, 1290, 1504
PciI ACATGT 1 cut(s) 1659
PcsI WCGNNNNNNNCGW 1 cut(s) 1627
PdmI GAANNNNTTC 3 cut(s) 161, 228, 669
PfeI GAWTC 2 cut(s) 705, 1033
PkrI GCNGC 6 cut(s) 48, 585, 1333, 1377, 1540, 1705
PleI GAGTC 3 cut(s) 211, 986, 1009
PpsI GAGTC 3 cut(s) 211, 986, 1009
PscI ACATGT 1 cut(s) 1659
PsiI TTATAA 2 cut(s) 470, 1107
Psp6I CCWGG 3 cut(s) 570, 1068, 1528
PspFI CCCAGC 1 cut(s) 586
PspGI CCWGG 3 cut(s) 570, 1068, 1528
PspN4I GGNNCC 1 cut(s) 1608
PstI CTGCAG 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 2 cut(s) 870, 1164
PvuII CAGCTG 3 cut(s) 69, 586, 1706
RsaI GTAC 9 cut(s) 337, 1024, 1086, 1100, 1196, 1599, 1620, 1658, 1711
RsaNI GTAC 9 cut(s) 336, 1023, 1085, 1099, 1195, 1598, 1619, 1657, 1710
RseI CAYNNNNRTG 3 cut(s) 780, 1226, 1465
SaqAI TTAA 7 cut(s) 192, 533, 792, 879, 888, 1575, 1722
SatI GCNGC 6 cut(s) 47, 584, 1332, 1376, 1539, 1704
Sau3AI GATC 6 cut(s) 546, 870, 1148, 1164, 1287, 1501
ScaI AGTACT 1 cut(s) 1196
SchI GAGTC 3 cut(s) 212, 987, 1009
ScrFI CCNGG 3 cut(s) 572, 1070, 1530
SduI GDGCHC 3 cut(s) 280, 456, 1667
SexAI ACCWGGT 1 cut(s) 1068
SfaNI GCATC 4 cut(s) 22, 706, 1354, 1374
SfcI CTRYAG 3 cut(s) 91, 416, 618
SfuI TTCGAA 1 cut(s) 1123
SmiMI CAYNNNNRTG 3 cut(s) 780, 1226, 1465
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SpeI ACTAGT 1 cut(s) 1712
SsiI CCGC 1 cut(s) 1331
SspI AATATT 1 cut(s) 1687
SspMI CTAG 7 cut(s) 537, 860, 1179, 1262, 1487, 1535, 1713
StyD4I CCNGG 3 cut(s) 570, 1068, 1528
TaaI ACNGT 6 cut(s) 136, 381, 1027, 1084, 1103, 1631
TaqI TCGA 2 cut(s) 1123, 1251
TatI WGTACW 3 cut(s) 1098, 1194, 1656
TauI GCSGC 1 cut(s) 1334
TfiI GAWTC 2 cut(s) 705, 1033
Tru1I TTAA 7 cut(s) 192, 533, 792, 879, 888, 1575, 1722
Tru9I TTAA 7 cut(s) 192, 533, 792, 879, 888, 1575, 1722
TscAI CASTG 2 cut(s) 290, 1087
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 5 cut(s) 46, 583, 1375, 1538, 1703
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 7 cut(s) 48, 74, 131, 258, 644, 918, 949
TspRI CASTG 2 cut(s) 290, 1087
Vha464I CTTAAG 1 cut(s) 191
XbaI TCTAGA 1 cut(s) 1261
XceI RCATGY 1 cut(s) 1663
XmiI GTMKAC 2 cut(s) 374, 936
XmnI GAANNNNTTC 3 cut(s) 161, 228, 669
XspI CTAG 7 cut(s) 537, 860, 1179, 1262, 1487, 1535, 1713
ZrmI AGTACT 1 cut(s) 1196
Zsp2I ATGCAT 2 cut(s) 1369, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.