FvH4_6g36260

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007270
Physical Location & Seq
Forward (+)
28510632 .. 28519397
8766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36260.t1

Sequence Viewer

Length: 3471 bp
ATGGAGAAGTCTCAGAAGAAGACTAGCTTGCAACTCCAAGCCCTCGAAAGCTTCTATGAAGAGGAAAAGTATCCGAGTCGAACTGCTATGGAATGCCATGCCACAGTGTTTGGATTGACTTACAAGCAGGTTCGGGGTTGGTTTGTTGAGAGGAGGAGGAGGGAGAAGAGGAAACGTACAGCTGGTGAGTTGTTGAGTGGAAGAAATGGGGTGGGAGTTGCTGCTACTAGGGTAGCTAAAAGAAGTGACTCGAGTCAGAGAACAAAAGCTGCTGCTTCAGGTTTGAAATGCAAGAAGGGAGCCATAAAAGCTAGGATTCAGAGATTGCGAAATCCGGACTACCTTCTTAAGAAAGTGTTCCGCAAGGACGGCCCTCCAATCGCTGTGGAGTTTGATGCTCTTCCTTCTCGAGCACTGTGGAAGTCTACAGATTCCCAAAATGAGGAGCTAAATAGCAGTGCTCCAAGGAAAAGACATGGGGCCGGAAAAGACTTGATGACTATGAGAAAACAGGGTGTTGGGAAAGACTTAATGACAGTGAGAAGGCATAACGGTGGAAAAGACTTGATGAAAATGAAACAACATGGTTGTGGGAAAGACTTGATGACAATGAAAAAACATGGTGGCGGTAAAGGTTTGATGACAGTCTGGCGGGCAAACAATCCTGATGCTGATGCTAGAGACTTTCTCGTTGACATGGGTTTAGCTAATGGCGAAGTCACTCATGTATCACGGAAACCACAAACTCGGAGCAGAAGATTGCAACAACAAAAATCTGTTCCTAAACAGGGAAGGTTACAGAGCAAGTTACAAGAAAAGAGGAAGCGTTTTGTCAAAAGAAGAGAGGTGGAATATAATGAAGTCTCAAACCAGAAGCTGCCTAGCAAAGAAAAATGTGAACTTTCCTTGGAAGGATCAGGTTCTGAAGACCACTCGGATAAGATCGCAATGCTTGTGGATGATGAAGAGCTTGAGCTTAGAGAATTACAAGCAAGACCAATTTCACTGGGGTGCTTGAATCATTTTACTACTAATGGTGATCATGGTTGTTCACTTTGCAAAGATTCGCTGGTTAAGTTTCCTCCTAGTTCTGTCAAGATGAAGCAGCCATTTCATATGCAACCTTGGGATTCTTCACCAGAGATTGCCAAGAAATTGTTTAAGGTTTTTCATTTCCTTTATACTTATGTGGTTGTGTTAGATCTATCTTCCTTCACTGTGGATGAGTTCGCTCAAGCATTTCATGAGAAGGATTCCTTGCTACTTGGAAAGATACATGTGGCTCTTTTGAAGCTTCTTTTATCCCATGTTCAAGCGGAGCTATCCTCTGGATCTATGCATCACTTGAGCAAATCTTGCAATTTTCTCGCATTTATCCACTCGCAGCTTGAAAATCAAAAATCTACTTTGGAATTCTGGGAGAGATCTCTGAACCCCTTAACATGGACAGAAATACTTCGTCAAGTACTGGTTGCTGCTGGTTTTGGTTCAAAGCAGGGTGCGATGCGGAAGGAAGTCCTTAGCAAGGAAATGAGCCTTATGGTGAAGTATGGTTTGCATTCTGGTACTCTGAAGGGTGAACTATTTAGAGTTCTGCTAGAGCAAGGAATTAATGGTTTGAAAGTTTCCGACTTAGCGAAGTCATTGCAGATTGCTGAATTGAATGTTTCCAGCAGAATAGATGATTTGGAGTCTTTAATTAGTTCGACTCTTTCAAGTGATATTACATTATTTGAAAAGATTTCATCATCCACATACCGTTTACGCATCAATTCTTCTGAAGACGAAGTTGAGGAGCTTCAATCAGATTCTGAAGACTCTGGCACAGTTGATGATGACCTGAGTGACAGTGGTATATGTAGCAGTGATGATGATTCTGGGTGCAATTCAGGAAATCCCAATATCAGAAAATCGATTCATGTCAACCGCCATAGAAGTAAAACTAACATGCGGAAAGTACACACTGAAATTGATGAGAGCCATCCAGGAGAAGTATGGTTGTTAGGATTGATGGAAGGCGAGTATTCAGATTTAAGCATTGAAGAGAAGTTGAACGCGATAGTGGCTCTAATTGATCTTCTTCATGCAGGATCCAATATGAGAATGGAGGATCCTGCAAATAGTATTGCTGAATGCATTCCTAACAGCCTTCATTCTGGTTCTGGAGCGAAAATTAAGAGGTTATCAGCTAAACAGCATAGCGTCCCCAGGTCATCATGGGTCCATGCTGGAAATATGGATGGTGTAAATGGAGATCATACACGATCACTGTTCCACCCTATAGATTCTTCAGCATCAATCTCAAAATTTTATGGAGAGAGATACTCTACAAAAGGAAAATATTGCGGCTCTGACTTACACCCAATGCAATCTGTGTTTTTGGGGTCAGATCGTAGGTACAGTAGATACTGGCTTTTCTTGGGCCCTTGTAATGCATACGACCCGGGCCATAGAAGGGTTTATTTTGAATCTTCAGAAGATGGTCACTGGGAGGTTATTGATACCGAAGAGGCTTTATGTGCCTTGCTGTCAATTTTGGATGATAGGGGTAAAAGAGAGGCTTTTCTTATTGAATCTTTGGAGAAACGGCTTACATTTCTGTGTGAAGCAATGTCAAATACAACGGCAAGTAGTGATGGAAGTGAGAATTTGACGCAATCTGATCGCTCAGAACTGGATAATGCAAGAGAAGACACGTATTCACCAATATCTGATGTGGATAACAACTCTTCTGAGACTGTAAATGATTCTGTGCCTTTGAATGGAACTGAAGTTCCTGAAGTTCGAAAGAAAGGGGAAGAACTGCAACAGAAATGGAAACAAATCCAAGCATTTGATTCATGGTTATGGAATTCATTCTACCTAGATCTAAATTCCGTTAAACATGGTAAACGGTCATATTTCGATACACTTACTAGATGTGAAAGCTGTCACGATTTGTATTGGAGAGATGAGAAGCACTGCAGGATTTGCCATGCAACATTTGAGCTTCATTTTGACCAGGAAGAAATGTTCGCTATCCATGTAGCCACATGTAGGGAGAAGGAAACAAGTACTACGTTTCCTGAGCATAAGGTTCTCTCATCACAAATCCAATCTCTAAAAGCTGCGATCCATGCAATTGAGTCGGTTATGCCTGAAGATGCCTTGTTGGGTGCTTGGAAAAAATCTGCTCATAAGCTATGGGTGAAACGTCTTAGACGGACATCATCATTGTCAGAGCTATTGCAGGTTCTTACTGATTTTGTTAAAGCCATCAATGAGGACTGGCTGTATAAATGCAAAATTGCGCAAGGTTCTTGCAAGCTTGGTGACGAGATCATATCTTCTTTTGCGTCTATGCCTCATACAACATCTGCAGTTGCACTCTGGTTGGCAAAGTTGGATGACTTGATTGCTCCTTATATCAAAGGACCCTGTTCTGAGAGAAGGCAGGGGACCACCATTAGAGGTAAGCATCCAAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT Homeobox WHIM1 WSD

Protein Analysis

1157

Amino Acids

130.47

Weight (kDa)

8.5

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 8 - 57 2.7e-09 Homeodomain
DDT PF02791 384 - 436 1e-11 DDT domain
HARE-HTH PF05066 522 - 589 1.5e-06 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 661 - 694 1.3e-06 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 790 - 863 2.5e-20 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3291
Acc36I ACCTGC 2 cut(s) 118, 3220
AccI GTMKAC 1 cut(s) 425
AccII CGCG 1 cut(s) 2057
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 8 cut(s) 361, 627, 652, 1316, 1507, 1927, 1951, 2346
AclWI GGATC 7 cut(s) 922, 1339, 2085, 2098, 2105, 2118, 3106
AcsI RAATTY 5 cut(s) 1412, 2306, 2647, 2851, 2872
AfaI GTAC 6 cut(s) 178, 1467, 1567, 1959, 2399, 3055
AfiI CCNNNNNNNGG 7 cut(s) 442, 788, 1443, 1525, 2455, 2762, 3036
AflII CTTAAG 1 cut(s) 347
AflIII ACRYGT 3 cut(s) 1276, 2694, 3032
AjnI CCWGG 3 cut(s) 1984, 2207, 3000
AleI CACNNNNGTG 1 cut(s) 1009
AloI GAACNNNNNNTCC 6 cut(s) 2757, 2758, 2789, 2790, 2996, 3028
Alw21I GWGCWC 2 cut(s) 415, 463
Alw26I GTCTC 4 cut(s) 15, 675, 868, 2729
AlwI GGATC 7 cut(s) 922, 1339, 2085, 2098, 2105, 2118, 3106
AlwNI CAGNNNCTG 3 cut(s) 877, 923, 1811
Ama87I CYCGRG 3 cut(s) 250, 408, 2443
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 4 cut(s) 370, 480, 2422, 2446
ApaI GGGCCC 1 cut(s) 2426
ApeKI GCWGC 8 cut(s) 221, 269, 272, 877, 1105, 1384, 1475, 3107
ApoI RAATTY 5 cut(s) 1412, 2306, 2647, 2851, 2872
AseI ATTAAT 1 cut(s) 1611
Asp700I GAANNNNTTC 4 cut(s) 356, 1455, 2136, 2855
AspLEI GCGC 1 cut(s) 3292
AspS9I GGNCC 8 cut(s) 371, 480, 2221, 2422, 2423, 2446, 3413, 3438
AsuC2I CCSGG 2 cut(s) 2444, 2445
AsuHPI GGTGA 8 cut(s) 197, 1049, 1128, 1555, 1589, 2694, 3199, 3323
AsuII TTCGAA 1 cut(s) 2785
AvaI CYCGRG 3 cut(s) 250, 408, 2443
AvaII GGWCC 3 cut(s) 2221, 3413, 3438
BaeGI GKGCMC 1 cut(s) 2426
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BamHI GGATCC 2 cut(s) 2090, 2110
BanII GRGCYC 1 cut(s) 2426
BbsI GAAGAC 5 cut(s) 26, 933, 1788, 1821, 2697
Bbv12I GWGCWC 2 cut(s) 415, 463
BbvI GCAGC 8 cut(s) 208, 256, 259, 864, 1117, 1396, 1462, 3094
BccI CCATC 6 cut(s) 1989, 2005, 2234, 2474, 2630, 3263
BceAI ACGGC 3 cut(s) 385, 2603, 2640
BcgI CGANNNNNNTGC 8 cut(s) 1348, 1382, 1627, 1661, 2645, 2679, 3108, 3142
BciT130I CCWGG 3 cut(s) 1986, 2209, 3002
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 2 cut(s) 2444, 2445
BcoDI GTCTC 4 cut(s) 15, 675, 868, 2729
BfaI CTAG 9 cut(s) 24, 228, 312, 678, 882, 1086, 1598, 2864, 2916
BfmI CTRYAG 4 cut(s) 426, 2280, 2962, 3357
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 2 cut(s) 118, 3220
BfuI GTATCC 1 cut(s) 81
BglII AGATCT 3 cut(s) 1201, 1424, 2866
BisI GCNGC 9 cut(s) 222, 270, 273, 878, 1106, 1385, 1476, 2347, 3108
BlsI GCNGC 9 cut(s) 223, 271, 274, 879, 1107, 1386, 1477, 2348, 3109
BmcAI AGTACT 2 cut(s) 1467, 3055
Bme1390I CCNGG 5 cut(s) 1986, 2209, 2444, 2445, 3002
Bme18I GGWCC 3 cut(s) 2221, 3413, 3438
BmeT110I CYCGRG 3 cut(s) 250, 408, 2443
BmgT120I GGNCC 8 cut(s) 371, 480, 2221, 2422, 2423, 2446, 3413, 3438
BmiI GGNNCC 8 cut(s) 301, 481, 2092, 2112, 2222, 2424, 3415, 3439
BmrFI CCNGG 5 cut(s) 1986, 2209, 2444, 2445, 3002
BmrI ACTGGG 2 cut(s) 1016, 2497
BmsI GCATC 9 cut(s) 385, 658, 664, 1348, 1494, 1776, 2303, 3133, 3466
BmuI ACTGGG 2 cut(s) 1016, 2497
BoxI GACNNNNGTC 1 cut(s) 252
BpiI GAAGAC 5 cut(s) 26, 933, 1788, 1821, 2697
BplI GAGNNNNNCTC 6 cut(s) 672, 704, 1310, 1342, 2309, 2341
BpmI CTGGAG 1 cut(s) 2184
Bpu10I CCTNAGC 2 cut(s) 1520, 3066
Bpu14I TTCGAA 1 cut(s) 2785
BpuEI CTTGAG 3 cut(s) 992, 1218, 1366
BpuMI CCSGG 2 cut(s) 2444, 2445
Bsa29I ATCGAT 1 cut(s) 1913
BsaAI YACGTR 1 cut(s) 2697
BsaJI CCNNGG 5 cut(s) 464, 906, 1124, 2207, 2443
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 7 cut(s) 442, 788, 1443, 1525, 2455, 2762, 3036
Bse1I ACTGG 6 cut(s) 1011, 1473, 2414, 2492, 2679, 3272
Bse3DI GCAATG 3 cut(s) 954, 1643, 2616
BseAI TCCGGA 1 cut(s) 334
BseBI CCWGG 3 cut(s) 1986, 2209, 3002
BseCI ATCGAT 1 cut(s) 1913
BseDI CCNNGG 5 cut(s) 464, 906, 1124, 2207, 2443
BseGI GGATG 8 cut(s) 964, 1228, 1748, 1981, 2245, 2545, 3391, 3457
BseLI CCNNNNNNNGG 7 cut(s) 442, 788, 1443, 1525, 2455, 2762, 3036
BseMI GCAATG 3 cut(s) 954, 1643, 2616
BseMII CTCAG 6 cut(s) 26, 1832, 2682, 2724, 3057, 3414
BseNI ACTGG 6 cut(s) 1011, 1473, 2414, 2492, 2679, 3272
BseRI GAGGAG 5 cut(s) 166, 169, 172, 458, 1808
BseSI GKGCMC 1 cut(s) 2426
BseXI GCAGC 8 cut(s) 208, 256, 259, 864, 1117, 1396, 1462, 3094
Bsh1236I CGCG 1 cut(s) 2057
BshFI GGCC 4 cut(s) 372, 482, 2424, 2448
BshVI ATCGAT 1 cut(s) 1913
BsiHKAI GWGCWC 2 cut(s) 415, 463
BsiHKCI CYCGRG 3 cut(s) 250, 408, 2443
BsiSI CCGG 3 cut(s) 335, 483, 2444
BslFI GGGAC 2 cut(s) 2189, 3451
BslI CCNNNNNNNGG 7 cut(s) 442, 788, 1443, 1525, 2455, 2762, 3036
BsmAI GTCTC 4 cut(s) 15, 675, 868, 2729
BsmFI GGGAC 2 cut(s) 2189, 3451
BsmI GAATGC 4 cut(s) 98, 1558, 2136, 2138
BsnI GGCC 4 cut(s) 372, 482, 2424, 2448
BsoBI CYCGRG 3 cut(s) 250, 408, 2443
Bsp119I TTCGAA 1 cut(s) 2785
Bsp120I GGGCCC 1 cut(s) 2422
Bsp1286I GDGCHC 3 cut(s) 415, 463, 2426
Bsp13I TCCGGA 1 cut(s) 334
BspACI CCGC 8 cut(s) 361, 627, 652, 1316, 1507, 1927, 1951, 2346
BspANI GGCC 4 cut(s) 372, 482, 2424, 2448
BspCNI CTCAG 6 cut(s) 25, 1833, 2681, 2725, 3058, 3415
BspDI ATCGAT 1 cut(s) 1913
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 2057
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 8 cut(s) 301, 481, 2092, 2112, 2222, 2424, 3415, 3439
BspMAI CTGCAG 2 cut(s) 2966, 3361
BspMI ACCTGC 2 cut(s) 118, 3220
BspPI GGATC 7 cut(s) 922, 1339, 2085, 2098, 2105, 2118, 3106
BspQI GCTCTTC 2 cut(s) 405, 960
BspT104I TTCGAA 1 cut(s) 2785
BspTI CTTAAG 1 cut(s) 347
BsrDI GCAATG 3 cut(s) 954, 1643, 2616
BsrI ACTGG 6 cut(s) 1011, 1473, 2414, 2492, 2679, 3272
BssECI CCNNGG 5 cut(s) 464, 906, 1124, 2207, 2443
BssT1I CCWWGG 3 cut(s) 464, 906, 1124
Bst2UI CCWGG 3 cut(s) 1986, 2209, 3002
Bst6I CTCTTC 8 cut(s) 54, 161, 405, 835, 960, 2037, 2502, 2734
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 2 cut(s) 1356, 2970
BstBAI YACGTR 1 cut(s) 2697
BstBI TTCGAA 1 cut(s) 2785
BstC8I GCNNGC 3 cut(s) 29, 654, 3305
BstENI CCTNNNNNAGG 1 cut(s) 1523
BstF5I GGATG 8 cut(s) 964, 1228, 1748, 1981, 2245, 2545, 3391, 3457
BstFNI CGCG 1 cut(s) 2057
BstHHI GCGC 1 cut(s) 3292
BstMAI GTCTC 4 cut(s) 15, 675, 868, 2729
BstMWI GCNNNNNNNGC 8 cut(s) 308, 369, 1356, 2063, 2519, 2970, 3116, 3463
BstNI CCWGG 3 cut(s) 1986, 2209, 3002
BstNSI RCATGY 3 cut(s) 1280, 1951, 3036
BstPAI GACNNNNGTC 1 cut(s) 252
BstSCI CCNGG 5 cut(s) 1984, 2207, 2442, 2443, 3000
BstSFI CTRYAG 4 cut(s) 426, 2280, 2962, 3357
BstSLI GKGCMC 1 cut(s) 2426
BstUI CGCG 1 cut(s) 2057
BstV1I GCAGC 8 cut(s) 208, 256, 259, 864, 1117, 1396, 1462, 3094
BstV2I GAAGAC 5 cut(s) 26, 933, 1788, 1821, 2697
BstX2I RGATCY 6 cut(s) 1201, 1331, 1424, 2090, 2110, 2866
BstYI RGATCY 6 cut(s) 1201, 1331, 1424, 2090, 2110, 2866
Bsu15I ATCGAT 1 cut(s) 1913
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 4 cut(s) 372, 482, 2424, 2448
BsuTUI ATCGAT 1 cut(s) 1913
BtgZI GCGATG 1 cut(s) 1517
BtsCI GGATG 8 cut(s) 964, 1228, 1748, 1981, 2245, 2545, 3391, 3457
BtsI GCAGTG 3 cut(s) 463, 1870, 2959
BveI ACCTGC 2 cut(s) 118, 3220
Cac8I GCNNGC 3 cut(s) 29, 654, 3305
CaiI CAGNNNCTG 3 cut(s) 877, 923, 1811
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 1 cut(s) 3292
Cfr13I GGNCC 8 cut(s) 371, 480, 2221, 2422, 2423, 2446, 3413, 3438
Cfr9I CCCGGG 1 cut(s) 2443
ClaI ATCGAT 1 cut(s) 1913
CseI GACGC 3 cut(s) 2191, 2662, 3324
Csp6I GTAC 6 cut(s) 177, 1466, 1566, 1958, 2398, 3054
CspCI CAANNNNNGTGG 6 cut(s) 91, 126, 366, 401, 936, 971
CviQI GTAC 6 cut(s) 177, 1466, 1566, 1958, 2398, 3054
Eam1104I CTCTTC 8 cut(s) 54, 161, 405, 835, 960, 2037, 2502, 2734
EarI CTCTTC 8 cut(s) 54, 161, 405, 835, 960, 2037, 2502, 2734
Eco130I CCWWGG 3 cut(s) 464, 906, 1124
Eco24I GRGCYC 1 cut(s) 2426
Eco47I GGWCC 3 cut(s) 2221, 3413, 3438
Eco88I CYCGRG 3 cut(s) 250, 408, 2443
EcoNI CCTNNNNNAGG 1 cut(s) 1523
EcoO109I RGGNCCY 2 cut(s) 2423, 3413
EcoRI GAATTC 2 cut(s) 1412, 2851
EcoRII CCWGG 3 cut(s) 1984, 2207, 3000
EcoT14I CCWWGG 3 cut(s) 464, 906, 1124
EcoT22I ATGCAT 3 cut(s) 1341, 2138, 2437
EcoT38I GRGCYC 1 cut(s) 2426
ErhI CCWWGG 3 cut(s) 464, 906, 1124
FalI AAGNNNNNCTT 6 cut(s) 11, 43, 1241, 1273, 2545, 2577
FaqI GGGAC 2 cut(s) 2189, 3451
FauI CCCGC 1 cut(s) 645
FauNDI CATATG 1 cut(s) 1116
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 9 cut(s) 222, 270, 273, 878, 1106, 1385, 1476, 2347, 3108
FokI GGATG 8 cut(s) 971, 1235, 1735, 1968, 2252, 2552, 3398, 3444
FriOI GRGCYC 1 cut(s) 2426
Fsp4HI GCNGC 9 cut(s) 222, 270, 273, 878, 1106, 1385, 1476, 2347, 3108
FspBI CTAG 9 cut(s) 24, 228, 312, 678, 882, 1086, 1598, 2864, 2916
FspI TGCGCA 1 cut(s) 3291
GlaI GCGC 1 cut(s) 3291
GluI GCNGC 9 cut(s) 222, 270, 273, 878, 1106, 1385, 1476, 2347, 3108
GsuI CTGGAG 1 cut(s) 2184
HaeIII GGCC 4 cut(s) 372, 482, 2424, 2448
HapII CCGG 3 cut(s) 335, 483, 2444
HgaI GACGC 3 cut(s) 2191, 2662, 3324
HhaI GCGC 1 cut(s) 3292
Hin6I GCGC 1 cut(s) 3290
HinP1I GCGC 1 cut(s) 3290
HincII GTYRAC 2 cut(s) 694, 1924
HindII GTYRAC 2 cut(s) 694, 1924
HindIII AAGCTT 3 cut(s) 49, 1292, 3305
HpaII CCGG 3 cut(s) 335, 483, 2444
HphI GGTGA 8 cut(s) 197, 1049, 1128, 1555, 1589, 2694, 3199, 3323
Hpy166II GTNNAC 9 cut(s) 426, 694, 899, 1052, 1580, 1763, 1924, 1960, 2891
Hpy8I GTNNAC 9 cut(s) 426, 694, 899, 1052, 1580, 1763, 1924, 1960, 2891
HpyCH4IV ACGT 4 cut(s) 175, 2696, 3059, 3193
HpyF10VI GCNNNNNNNGC 8 cut(s) 308, 369, 1356, 2063, 2519, 2970, 3116, 3463
HpySE526I ACGT 4 cut(s) 175, 2696, 3059, 3193
HspAI GCGC 1 cut(s) 3290
Kpn2I TCCGGA 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 1039
LguI GCTCTTC 2 cut(s) 405, 960
LmnI GCTCC 8 cut(s) 299, 445, 466, 750, 1318, 1795, 2165, 3403
Lsp1109I GCAGC 8 cut(s) 208, 256, 259, 864, 1117, 1396, 1462, 3094
LweI GCATC 9 cut(s) 385, 658, 664, 1348, 1494, 1776, 2303, 3133, 3466
MaeI CTAG 9 cut(s) 24, 228, 312, 678, 882, 1086, 1598, 2864, 2916
MaeII ACGT 4 cut(s) 175, 2696, 3059, 3193
MaeIII GTNAC 8 cut(s) 245, 718, 795, 807, 1844, 2483, 2930, 3311
MfeI CAATTG 1 cut(s) 3120
MflI RGATCY 6 cut(s) 1201, 1331, 1424, 2090, 2110, 2866
MhlI GDGCHC 3 cut(s) 415, 463, 2426
MlyI GAGTC 7 cut(s) 85, 242, 262, 1700, 1702, 1811, 3134
MmeI TCCRAC 2 cut(s) 1653, 3363
Mph1103I ATGCAT 3 cut(s) 1341, 2138, 2437
MroI TCCGGA 1 cut(s) 334
MroXI GAANNNNTTC 4 cut(s) 356, 1455, 2136, 2855
MslI CAYNNNNRTG 5 cut(s) 552, 588, 1009, 2599, 2845
MspA1I CMGCKG 1 cut(s) 182
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 3 cut(s) 335, 483, 2444
MspR9I CCNGG 5 cut(s) 1986, 2209, 2444, 2445, 3002
MunI CAATTG 1 cut(s) 3120
Mva1269I GAATGC 4 cut(s) 98, 1558, 2136, 2138
MvaI CCWGG 3 cut(s) 1986, 2209, 3002
MvnI CGCG 1 cut(s) 2057
MwoI GCNNNNNNNGC 8 cut(s) 308, 369, 1356, 2063, 2519, 2970, 3116, 3463
NciI CCSGG 2 cut(s) 2444, 2445
NdeI CATATG 1 cut(s) 1116
NlaIV GGNNCC 8 cut(s) 301, 481, 2092, 2112, 2222, 2424, 3415, 3439
NmuCI GTSAC 6 cut(s) 245, 718, 1844, 2483, 2930, 3311
NsbI TGCGCA 1 cut(s) 3291
NsiI ATGCAT 3 cut(s) 1341, 2138, 2437
NspI RCATGY 3 cut(s) 1280, 1951, 3036
NspV TTCGAA 1 cut(s) 2785
OliI CACNNNNGTG 1 cut(s) 1009
PaeR7I CTCGAG 2 cut(s) 250, 408
PagI TCATGA 1 cut(s) 1243
PciI ACATGT 2 cut(s) 1276, 3032
PciSI GCTCTTC 2 cut(s) 405, 960
PctI GAATGC 4 cut(s) 98, 1558, 2136, 2138
PdmI GAANNNNTTC 4 cut(s) 356, 1455, 2136, 2855
PfoI TCCNGGA 1 cut(s) 1984
PkrI GCNGC 9 cut(s) 223, 271, 274, 879, 1107, 1386, 1477, 2348, 3109
PleI GAGTC 7 cut(s) 84, 242, 261, 1699, 1702, 1811, 3133
PpsI GAGTC 7 cut(s) 84, 242, 261, 1699, 1702, 1811, 3133
Ppu21I YACGTR 1 cut(s) 2697
PpuMI RGGWCCY 1 cut(s) 3413
PscI ACATGT 2 cut(s) 1276, 3032
PshAI GACNNNNGTC 1 cut(s) 252
PshBI ATTAAT 1 cut(s) 1611
Psp5II RGGWCCY 1 cut(s) 3413
Psp6I CCWGG 3 cut(s) 1984, 2207, 3000
PspGI CCWGG 3 cut(s) 1984, 2207, 3000
PspN4I GGNNCC 8 cut(s) 301, 481, 2092, 2112, 2222, 2424, 3415, 3439
PspOMI GGGCCC 1 cut(s) 2422
PspPI GGNCC 8 cut(s) 371, 480, 2221, 2422, 2423, 2446, 3413, 3438
PspPPI RGGWCCY 1 cut(s) 3413
PspXI VCTCGAGB 1 cut(s) 250
PstI CTGCAG 2 cut(s) 2966, 3361
PstNI CAGNNNCTG 3 cut(s) 877, 923, 1811
PsuI RGATCY 6 cut(s) 1201, 1331, 1424, 2090, 2110, 2866
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 6 cut(s) 178, 1467, 1567, 1959, 2399, 3055
RsaNI GTAC 6 cut(s) 177, 1466, 1566, 1958, 2398, 3054
RseI CAYNNNNRTG 5 cut(s) 552, 588, 1009, 2599, 2845
SapI GCTCTTC 2 cut(s) 405, 960
SatI GCNGC 9 cut(s) 222, 270, 273, 878, 1106, 1385, 1476, 2347, 3108
Sau96I GGNCC 8 cut(s) 371, 480, 2221, 2422, 2423, 2446, 3413, 3438
ScaI AGTACT 2 cut(s) 1467, 3055
SchI GAGTC 7 cut(s) 85, 242, 262, 1700, 1702, 1811, 3134
ScrFI CCNGG 5 cut(s) 1986, 2209, 2444, 2445, 3002
SduI GDGCHC 3 cut(s) 415, 463, 2426
SfaNI GCATC 9 cut(s) 385, 658, 664, 1348, 1494, 1776, 2303, 3133, 3466
SfcI CTRYAG 4 cut(s) 426, 2280, 2962, 3357
Sfr274I CTCGAG 2 cut(s) 250, 408
SfuI TTCGAA 1 cut(s) 2785
SinI GGWCC 3 cut(s) 2221, 3413, 3438
SlaI CTCGAG 2 cut(s) 250, 408
SmaI CCCGGG 1 cut(s) 2445
SmiMI CAYNNNNRTG 5 cut(s) 552, 588, 1009, 2599, 2845
SmlI CTYRAG 6 cut(s) 250, 347, 408, 971, 1233, 1345
SmoI CTYRAG 6 cut(s) 250, 347, 408, 971, 1233, 1345
SsiI CCGC 8 cut(s) 361, 627, 652, 1316, 1507, 1927, 1951, 2346
SspI AATATT 1 cut(s) 2342
SspMI CTAG 9 cut(s) 24, 228, 312, 678, 882, 1086, 1598, 2864, 2916
StyD4I CCNGG 5 cut(s) 1984, 2207, 2442, 2443, 3000
StyI CCWWGG 3 cut(s) 464, 906, 1124
TaiI ACGT 4 cut(s) 178, 2699, 3062, 3196
TaqI TCGA 8 cut(s) 45, 79, 251, 409, 1706, 1913, 2785, 2904
TatI WGTACW 3 cut(s) 1465, 1957, 3053
TauI GCSGC 1 cut(s) 2349
TseFI GTSAC 6 cut(s) 245, 718, 1844, 2483, 2930, 3311
TseI GCWGC 8 cut(s) 221, 269, 272, 877, 1105, 1384, 1475, 3107
Tsp45I GTSAC 6 cut(s) 245, 718, 1844, 2483, 2930, 3311
TspGWI ACGGA 3 cut(s) 748, 2866, 3217
TspMI CCCGGG 1 cut(s) 2443
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 3 cut(s) 2221, 3413, 3438
VspI ATTAAT 1 cut(s) 1611
XagI CCTNNNNNAGG 1 cut(s) 1523
XapI RAATTY 5 cut(s) 1412, 2306, 2647, 2851, 2872
XceI RCATGY 3 cut(s) 1280, 1951, 3036
XcmI CCANNNNNNNNNTGG 2 cut(s) 1992, 2101
XhoI CTCGAG 2 cut(s) 250, 408
XmaI CCCGGG 1 cut(s) 2443
XmiI GTMKAC 1 cut(s) 425
XmnI GAANNNNTTC 4 cut(s) 356, 1455, 2136, 2855
XspI CTAG 9 cut(s) 24, 228, 312, 678, 882, 1086, 1598, 2864, 2916
ZrmI AGTACT 2 cut(s) 1467, 3055
Zsp2I ATGCAT 3 cut(s) 1341, 2138, 2437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.