Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0012863

Physical Location & Seq

Genomic Coordinates Reverse (-)

29490470 .. 29494799

Gene Structure 4330 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g37460.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1908 bp

ATGGCTTCTTCTTCTTCTTCCCTCAAAGCCATCTCCACCTTCACCACCACCCCTTCACAAACACCACCTCCTTCATCATTATCAAAAGCTTCCCTTGTCTCCTTCCTCAACACCACCACCTCCTCTCCCATCTCCCTAAAGCTCTCTCATTCCTTCTCTCCTTCACATTTACGTTCCAATGACTCCACTCTATCTCTCCTAGTCACCAACGCTCTCAAAACCGCAGAGTCTTCATCTTTATCATCGTCCAACCTCACAAAGTCTCCCTCTTCCGACCCCAAGCCGACGATCCTCGTCTCCGAGAAGCTTGGAGAAGCAGGGCTGGAGGTCCTACGCGCGTTCGGAAACCTCGAGTGCGTGTACAACCTAACGCCGGAGGAGCTCTGTCTGAAGATCTCAGCATGCGACGCGCTGATCGTGAGGAGCGGGACGAAGGTGACTAGAGAGGTTTTTGAGGCGTCGAAAGGGAGGCTGAAGGTGGTGGGGAGAGCAGGGGTTGGTATAGACAATGTGGATCTGCAGGCGGCGACGGAGTTCGGATGCCTCGTCGTTAATGCGCCGACGGCTAACACCATCGCCGCCGCAGAGCACGGCATCGCGCTGCTCACTTCCATGGCTAGAAATGTTGCTCAGGCAGATGCCTCCATGAAAGCTGGACTATGGAAGCGAAACAAGTATGTTGGTGTCTCTCTTGTTGGGAAGACATTAGCAGTAATGGGGTTCGGAAAAGTTGGTTCTGAAGTTGCTAGACGTGCAAAAGGCTTGGGAATGCATGTTATTGCTCATGATCCATATGCCCCAGCTGACAGAGCTCGTGCCATTGGTGTGGATTTGGTCTCTTTCGACCAGGCCATCTCCACTGCAGATTTCGTGTCTCTGCATATGCCACTCACCCCTACTACAGCAAAGGTGTTCAATGATGATACGTTTTCAAAGATGAAGAAAGGTGTTCGCATTATTAATGTTGCAAGGGGTGGAGTCATCGATGAAGATGCATTAGTGCGAGCGCTTGATAGTGGAGTTGTAGCTCAGGCTGCACTTGATGTGTTCACAGAGGAACCTCCCTCAAAAGAAAGCAAGTTGGTGCAACATGAGAATGTCACAGTCACACCTCATCTTGGAGCTAGCACAAAGGAAGCACAGGAAGGTGTAGCAATTGAAATAGCCGAGGCTGTAGTTGGAGCATTACAAGGGGAACTTTCTGCAACCGCTGTCAATGCACCCATGGTTCCTCCTGAGGTTATGTCGGAGTTGTCTCCTTATGTTGTTCTTGCTGAGAAGCTTGGAAAGTTGGCTGTACAATTAGTAGCAGGAGGGAGTGGCATCACATCTGTGAGGGTGGTATACAAATCAGCTAGGGATCCTGATGACTTGGATACTAGACTTCTCCGGGCCATGATCACTAAAGGCATCTTTGAACCGATATCTACCTCATTCATCAATCTAGTAAATGCTGATTTTGTTGCCAAGCAGAAAGGTCTGCGTATAAGTGAGGAAAAGGTAACTGTCGACTCTTCTCCTGAGTTCCCTGTTGACTCAATCCAGGTGCACATATCCAATGTGGACTCCAAATTTGCAAGTTCAGTTTCTGAAAATGGAAGCATAAGCATTGAGGGGAAAGTGAAATATGGTGAACCCCATCTCACATGTTTGGGATCATTTGGCGTGGACGTGAGCCTTGAAGGGAATCTTATTTTGTGCCGGCAGGTAGATCAACCAGGAATGATTGGCAAGGTGGGAAATATTCTTGGAACTCAAAATGTGAATGTGAGCTTCATGAGTGTGGGTAGAACCATCCTGAAGAAAGCCATCATGGCTATTGGTGTAGATGAGGAACCAAGCAAGGAGACCTTAACGAAAATTGGGGATGTTTCTGCTATTGAAGAGTTTGTGTTCCTTAAGCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

636

Amino Acids

66.88

Weight (kDa)

6.13

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	97 - 406	2.1e-34	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	199 - 374	6.2e-63	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter	PF19304	418 - 522	8.3e-14	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	565 - 623	3.7e-08	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17745 AT1G17745
fragaria_vesca	FvH4_6g37460
malus_domestica	MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica	Prupe.3G168100_v2.0.a1
pyrus_communis	pycom09g07670 pycom17g13710
rosa_chinensis	RchiOBHm_Chr2g0150301
rosa_laevigata	RLG00000020484
rosa_multiflora	Rmu_sc0002335.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097310
rosa_rugosa	Rorug02G0420400
rosa_samantha	Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana	Rw2G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1696
AccBSI	CCGCTC	1 cut(s)	426
AccI	GTMKAC	2 cut(s)	1344, 1509
AccII	CGCG	4 cut(s)	336, 338, 410, 599
AciI	CCGC	6 cut(s)	222, 426, 524, 579, 582, 1209
AclWI	GGATC	6 cut(s)	283, 522, 782, 1355, 1368, 1663
AcsI	RAATTY	1 cut(s)	1571
AcuI	CTGAAG	4 cut(s)	410, 494, 759, 1820
AcyI	GRCGYC	1 cut(s)	458
AfaI	GTAC	2 cut(s)	362, 1299
AfeI	AGCGCT	1 cut(s)	1008
AfiI	CCNNNNNNNGG	2 cut(s)	373, 1118
AflII	CTTAAG	1 cut(s)	1898
AflIII	ACRYGT	1 cut(s)	1646
AgsI	TTSAA	6 cut(s)	916, 933, 1160, 1418, 1682, 1883
AjiI	CACGTC	2 cut(s)	752, 1672
AjnI	CCWGG	3 cut(s)	846, 1542, 1717
AleI	CACNNNNGTG	1 cut(s)	1331
Alw21I	GWGCWC	4 cut(s)	384, 591, 814, 1551
Alw26I	GTCTC	8 cut(s)	103, 267, 301, 691, 841, 879, 1260, 1841
Alw44I	GTGCAC	1 cut(s)	1547
AlwI	GGATC	6 cut(s)	283, 522, 782, 1355, 1368, 1663
AlwNI	CAGNNNCTG	1 cut(s)	1589
Ama87I	CYCGRG	1 cut(s)	350
Aor51HI	AGCGCT	1 cut(s)	1008
AoxI	GGCC	2 cut(s)	849, 1392
ApaLI	GTGCAC	1 cut(s)	1547
ApeKI	GCWGC	2 cut(s)	601, 1034
ApoI	RAATTY	1 cut(s)	1571
ArsI	GACNNNNNNTTYG	2 cut(s)	1556, 1588
AseI	ATTAAT	1 cut(s)	960
AspLEI	GCGC	5 cut(s)	338, 412, 559, 601, 1009
AspS9I	GGNCC	2 cut(s)	328, 1392
AsuC2I	CCSGG	1 cut(s)	1391
AsuHPI	GGTGA	5 cut(s)	34, 196, 448, 883, 1643
AsuNHI	GCTAGC	1 cut(s)	1124
AvaI	CYCGRG	1 cut(s)	350
AvaII	GGWCC	1 cut(s)	328
AxyI	CCTNAGG	1 cut(s)	1236
BaeGI	GKGCMC	1 cut(s)	1551
BamHI	GGATCC	1 cut(s)	1360
BanII	GRGCYC	2 cut(s)	384, 814
BauI	CACGAG	1 cut(s)	813
BbsI	GAAGAC	2 cut(s)	222, 707
Bbv12I	GWGCWC	4 cut(s)	384, 591, 814, 1551
BbvI	GCAGC	2 cut(s)	588, 1021
BccI	CCATC	7 cut(s)	38, 137, 581, 860, 1647, 1802, 1817
BceAI	ACGGC	2 cut(s)	579, 607
BcgI	CGANNNNNNTGC	2 cut(s)	974, 1008
BciT130I	CCWGG	3 cut(s)	848, 1544, 1719
BciVI	GTATCC	1 cut(s)	1369
BclI	TGATCA	1 cut(s)	1398
BcnI	CCSGG	1 cut(s)	1391
BcoDI	GTCTC	8 cut(s)	103, 267, 301, 691, 841, 879, 1260, 1841
BfaI	CTAG	8 cut(s)	200, 441, 618, 747, 1125, 1356, 1380, 1445
BfmI	CTRYAG	5 cut(s)	518, 861, 900, 1173, 1904
BfoI	RGCGCY	1 cut(s)	1010
BfrI	CTTAAG	1 cut(s)	1898
BfuAI	ACCTGC	1 cut(s)	1696
BfuI	GTATCC	1 cut(s)	1369
BglI	GCCNNNNNGGC	1 cut(s)	1814
BglII	AGATCT	1 cut(s)	393
BisI	GCNGC	5 cut(s)	525, 579, 582, 602, 1035
BlsI	GCNGC	5 cut(s)	526, 580, 583, 603, 1036
Bme1390I	CCNGG	4 cut(s)	848, 1391, 1544, 1719
Bme18I	GGWCC	1 cut(s)	328
BmeT110I	CYCGRG	1 cut(s)	350
BmgBI	CACGTC	2 cut(s)	752, 1672
BmgT120I	GGNCC	2 cut(s)	328, 1392
BmiI	GGNNCC	4 cut(s)	1059, 1230, 1362, 1836
BmrFI	CCNGG	4 cut(s)	848, 1391, 1544, 1719
BmsI	GCATC	6 cut(s)	530, 603, 628, 982, 1332, 1419
BmtI	GCTAGC	1 cut(s)	1128
BpiI	GAAGAC	2 cut(s)	222, 707
BpmI	CTGGAG	1 cut(s)	344
Bpu10I	CCTNAGC	2 cut(s)	630, 1029
BpuMI	CCSGG	1 cut(s)	1391
Bsa29I	ATCGAT	1 cut(s)	984
BsaHI	GRCGYC	1 cut(s)	458
BsaI	GGTCTC	2 cut(s)	841, 1841
BsaJI	CCNNGG	3 cut(s)	612, 1167, 1224
BsaXI	ACNNNNNCTCC	6 cut(s)	52, 82, 109, 139, 247, 277
Bsc4I	CCNNNNNNNGG	2 cut(s)	373, 1118
Bse118I	RCCGGY	1 cut(s)	1701
Bse21I	CCTNAGG	1 cut(s)	1236
BseBI	CCWGG	3 cut(s)	848, 1544, 1719
BseCI	ATCGAT	1 cut(s)	984
BseDI	CCNNGG	3 cut(s)	612, 1167, 1224
BseGI	GGATG	3 cut(s)	545, 1794, 1873
BseLI	CCNNNNNNNGG	2 cut(s)	373, 1118
BseMII	CTCAG	6 cut(s)	411, 644, 1043, 1227, 1266, 1512
BseRI	GAGGAG	3 cut(s)	112, 392, 436
BseSI	GKGCMC	1 cut(s)	1551
BseXI	GCAGC	2 cut(s)	588, 1021
BseYI	CCCAGC	1 cut(s)	799
BsgI	GTGCAG	1 cut(s)	1020
Bsh1236I	CGCG	4 cut(s)	336, 338, 410, 599
BshFI	GGCC	2 cut(s)	851, 1394
BshVI	ATCGAT	1 cut(s)	984
BsiHKAI	GWGCWC	4 cut(s)	384, 591, 814, 1551
BsiHKCI	CYCGRG	1 cut(s)	350
BsiSI	CCGG	3 cut(s)	374, 1390, 1702
BslFI	GGGAC	1 cut(s)	442
BslI	CCNNNNNNNGG	2 cut(s)	373, 1118
BsmAI	GTCTC	8 cut(s)	103, 267, 301, 691, 841, 879, 1260, 1841
BsmBI	CGTCTC	1 cut(s)	301
BsmFI	GGGAC	1 cut(s)	442
BsmI	GAATGC	1 cut(s)	774
BsnI	GGCC	2 cut(s)	851, 1394
Bso31I	GGTCTC	2 cut(s)	841, 1841
BsoBI	CYCGRG	1 cut(s)	350
Bsp1286I	GDGCHC	4 cut(s)	384, 591, 814, 1551
Bsp1407I	TGTACA	2 cut(s)	360, 1297
Bsp143I	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
Bsp19I	CCATGG	2 cut(s)	612, 1224
BspACI	CCGC	6 cut(s)	222, 426, 524, 579, 582, 1209
BspANI	GGCC	2 cut(s)	851, 1394
BspCNI	CTCAG	6 cut(s)	410, 643, 1042, 1228, 1267, 1513
BspDI	ATCGAT	1 cut(s)	984
BspFNI	CGCG	4 cut(s)	336, 338, 410, 599
BspHI	TCATGA	2 cut(s)	784, 1776
BspLI	GGNNCC	4 cut(s)	1059, 1230, 1362, 1836
BspMAI	CTGCAG	2 cut(s)	522, 865
BspMI	ACCTGC	1 cut(s)	1696
BspOI	GCTAGC	1 cut(s)	1128
BspPI	GGATC	6 cut(s)	283, 522, 782, 1355, 1368, 1663
BspTI	CTTAAG	1 cut(s)	1898
BspTNI	GGTCTC	2 cut(s)	841, 1841
BsrBI	CCGCTC	1 cut(s)	426
BsrFI	RCCGGY	1 cut(s)	1701
BsrGI	TGTACA	2 cut(s)	360, 1297
BssAI	RCCGGY	1 cut(s)	1701
BssECI	CCNNGG	3 cut(s)	612, 1167, 1224
BssMI	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
BssNAI	GTATAC	1 cut(s)	1345
BssNI	GRCGYC	1 cut(s)	458
BssSI	CACGAG	1 cut(s)	813
BssT1I	CCWWGG	2 cut(s)	612, 1224
Bst1107I	GTATAC	1 cut(s)	1345
Bst2BI	CACGAG	1 cut(s)	813
Bst2UI	CCWGG	3 cut(s)	848, 1544, 1719
Bst4CI	ACNGT	2 cut(s)	1105, 1507
Bst6I	CTCTTC	3 cut(s)	274, 1519, 1878
BstACI	GRCGYC	1 cut(s)	458
BstAFI	CTTAAG	1 cut(s)	1898
BstAUI	TGTACA	2 cut(s)	360, 1297
BstC8I	GCNNGC	5 cut(s)	403, 522, 1005, 1126, 1703
BstDEI	CTNAG	6 cut(s)	397, 630, 1029, 1236, 1275, 1521
BstDSI	CCRYGG	2 cut(s)	612, 1224
BstF5I	GGATG	3 cut(s)	545, 1794, 1873
BstFNI	CGCG	4 cut(s)	336, 338, 410, 599
BstH2I	RGCGCY	1 cut(s)	1010
BstHHI	GCGC	5 cut(s)	338, 412, 559, 601, 1009
BstKTI	GATC	9 cut(s)	291, 396, 417, 517, 790, 1363, 1401, 1658, 1714
BstMAI	GTCTC	8 cut(s)	103, 267, 301, 691, 841, 879, 1260, 1841
BstMBI	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
BstMWI	GCNNNNNNNGC	8 cut(s)	379, 407, 563, 752, 809, 1034, 1217, 1814
BstNI	CCWGG	3 cut(s)	848, 1544, 1719
BstNSI	RCATGY	3 cut(s)	405, 776, 1650
BstSCI	CCNGG	4 cut(s)	846, 1389, 1542, 1717
BstSFI	CTRYAG	5 cut(s)	518, 861, 900, 1173, 1904
BstSLI	GKGCMC	1 cut(s)	1551
BstUI	CGCG	4 cut(s)	336, 338, 410, 599
BstV1I	GCAGC	2 cut(s)	588, 1021
BstV2I	GAAGAC	2 cut(s)	222, 707
BstX2I	RGATCY	3 cut(s)	393, 514, 1360
BstXI	CCANNNNNNTGG	1 cut(s)	826
BstYI	RGATCY	3 cut(s)	393, 514, 1360
BstZ17I	GTATAC	1 cut(s)	1345
Bsu15I	ATCGAT	1 cut(s)	984
Bsu36I	CCTNAGG	1 cut(s)	1236
BsuI	GTATCC	1 cut(s)	1369
BsuRI	GGCC	2 cut(s)	851, 1394
BsuTUI	ATCGAT	1 cut(s)	984
BtgI	CCRYGG	2 cut(s)	612, 1224
BtgZI	GCGATG	2 cut(s)	559, 580
BtrI	CACGTC	2 cut(s)	752, 1672
BtsCI	GGATG	3 cut(s)	545, 1794, 1873
BtsI	GCAGTG	1 cut(s)	858
BtsIMutI	CAGTG	1 cut(s)	858
BveI	ACCTGC	1 cut(s)	1696
Cac8I	GCNNGC	5 cut(s)	403, 522, 1005, 1126, 1703
CaiI	CAGNNNCTG	1 cut(s)	1589
CciI	TCATGA	2 cut(s)	784, 1776
CfoI	GCGC	5 cut(s)	338, 412, 559, 601, 1009
Cfr10I	RCCGGY	1 cut(s)	1701
Cfr13I	GGNCC	2 cut(s)	328, 1392
ClaI	ATCGAT	1 cut(s)	984
CseI	GACGC	2 cut(s)	416, 447
Csp6I	GTAC	2 cut(s)	361, 1298
CviQI	GTAC	2 cut(s)	361, 1298
DdeI	CTNAG	6 cut(s)	397, 630, 1029, 1236, 1275, 1521
DpnI	GATC	9 cut(s)	290, 395, 416, 516, 789, 1362, 1400, 1657, 1713
DpnII	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
Eam1104I	CTCTTC	3 cut(s)	274, 1519, 1878
EarI	CTCTTC	3 cut(s)	274, 1519, 1878
Ecl136II	GAGCTC	2 cut(s)	382, 812
Eco130I	CCWWGG	2 cut(s)	612, 1224
Eco24I	GRGCYC	2 cut(s)	384, 814
Eco31I	GGTCTC	2 cut(s)	841, 1841
Eco32I	GATATC	1 cut(s)	1425
Eco47I	GGWCC	1 cut(s)	328
Eco47III	AGCGCT	1 cut(s)	1008
Eco53kI	GAGCTC	2 cut(s)	382, 812
Eco57I	CTGAAG	4 cut(s)	410, 494, 759, 1820
Eco81I	CCTNAGG	1 cut(s)	1236
Eco88I	CYCGRG	1 cut(s)	350
EcoICRI	GAGCTC	2 cut(s)	382, 812
EcoO109I	RGGNCCY	1 cut(s)	328
EcoRII	CCWGG	3 cut(s)	846, 1542, 1717
EcoRV	GATATC	1 cut(s)	1425
EcoT14I	CCWWGG	2 cut(s)	612, 1224
EcoT22I	ATGCAT	2 cut(s)	774, 997
EcoT38I	GRGCYC	2 cut(s)	384, 814
ErhI	CCWWGG	2 cut(s)	612, 1224
Esp3I	CGTCTC	1 cut(s)	301
FalI	AAGNNNNNCTT	8 cut(s)	78, 110, 1182, 1214, 1674, 1706, 1835, 1867
FaqI	GGGAC	1 cut(s)	442
FauI	CCCGC	1 cut(s)	419
FauNDI	CATATG	2 cut(s)	793, 882
FbaI	TGATCA	1 cut(s)	1398
FblI	GTMKAC	2 cut(s)	1344, 1509
Fnu4HI	GCNGC	5 cut(s)	525, 579, 582, 602, 1035
FokI	GGATG	3 cut(s)	552, 1781, 1880
FriOI	GRGCYC	2 cut(s)	384, 814
Fsp4HI	GCNGC	5 cut(s)	525, 579, 582, 602, 1035
FspBI	CTAG	8 cut(s)	200, 441, 618, 747, 1125, 1356, 1380, 1445
GlaI	GCGC	5 cut(s)	337, 411, 558, 600, 1008
GluI	GCNGC	5 cut(s)	525, 579, 582, 602, 1035
GsaI	CCCAGC	1 cut(s)	803
GsuI	CTGGAG	1 cut(s)	344
HaeII	RGCGCY	1 cut(s)	1010
HaeIII	GGCC	2 cut(s)	851, 1394
HapII	CCGG	3 cut(s)	374, 1390, 1702
HgaI	GACGC	2 cut(s)	416, 447
HhaI	GCGC	5 cut(s)	338, 412, 559, 601, 1009
Hin1I	GRCGYC	1 cut(s)	458
Hin6I	GCGC	5 cut(s)	336, 410, 557, 599, 1007
HinP1I	GCGC	5 cut(s)	336, 410, 557, 599, 1007
HincII	GTYRAC	2 cut(s)	1510, 1534
HindII	GTYRAC	2 cut(s)	1510, 1534
HindIII	AAGCTT	3 cut(s)	87, 305, 1280
HinfI	GANTC	7 cut(s)	182, 227, 978, 1511, 1535, 1565, 1687
HpaII	CCGG	3 cut(s)	374, 1390, 1702
HphI	GGTGA	5 cut(s)	34, 196, 448, 883, 1643
Hpy166II	GTNNAC	9 cut(s)	361, 1050, 1345, 1510, 1534, 1549, 1564, 1634, 1669
Hpy188I	TCNGA	9 cut(s)	274, 301, 344, 390, 539, 725, 739, 1249, 1591
Hpy188III	TCNNGA	7 cut(s)	418, 785, 1235, 1364, 1520, 1777, 1798
Hpy8I	GTNNAC	9 cut(s)	361, 1050, 1345, 1510, 1534, 1549, 1564, 1634, 1669
Hpy99I	CGWCG	6 cut(s)	289, 410, 463, 532, 551, 565
HpyCH4III	ACNGT	2 cut(s)	1105, 1507
HpyCH4IV	ACGT	4 cut(s)	172, 751, 926, 1671
HpyF10VI	GCNNNNNNNGC	8 cut(s)	379, 407, 563, 752, 809, 1034, 1217, 1814
HpyF3I	CTNAG	6 cut(s)	397, 630, 1029, 1236, 1275, 1521
HpySE526I	ACGT	4 cut(s)	172, 751, 926, 1671
Hsp92I	GRCGYC	1 cut(s)	458
HspAI	GCGC	5 cut(s)	336, 410, 557, 599, 1007
KroI	GCCGGC	1 cut(s)	1701
KroNI	GCCGGC	1 cut(s)	1703
Ksp22I	TGATCA	1 cut(s)	1398
Kzo9I	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
LmnI	GCTCC	4 cut(s)	379, 423, 1121, 1181
Lsp1109I	GCAGC	2 cut(s)	588, 1021
LweI	GCATC	6 cut(s)	530, 603, 628, 982, 1332, 1419
MaeI	CTAG	8 cut(s)	200, 441, 618, 747, 1125, 1356, 1380, 1445
MaeII	ACGT	4 cut(s)	172, 751, 926, 1671
MaeIII	GTNAC	5 cut(s)	202, 436, 1099, 1105, 1501
MalI	GATC	9 cut(s)	290, 395, 416, 516, 789, 1362, 1400, 1657, 1713
MbiI	CCGCTC	1 cut(s)	426
MboI	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
MfeI	CAATTG	1 cut(s)	1155
MflI	RGATCY	3 cut(s)	393, 514, 1360
MhlI	GDGCHC	4 cut(s)	384, 591, 814, 1551
MluCI	AATT	4 cut(s)	1155, 1301, 1571, 1860
MlyI	GAGTC	6 cut(s)	176, 236, 987, 1505, 1529, 1559
MmeI	TCCRAC	4 cut(s)	273, 297, 1159, 1227
Mph1103I	ATGCAT	2 cut(s)	774, 997
MroNI	GCCGGC	1 cut(s)	1701
MseI	TTAA	4 cut(s)	552, 960, 1853, 1899
MslI	CAYNNNNRTG	5 cut(s)	611, 824, 1095, 1331, 1781
MspA1I	CMGCKG	2 cut(s)	803, 1211
MspCI	CTTAAG	1 cut(s)	1898
MspI	CCGG	3 cut(s)	374, 1390, 1702
MspR9I	CCNGG	4 cut(s)	848, 1391, 1544, 1719
MunI	CAATTG	1 cut(s)	1155
Mva1269I	GAATGC	1 cut(s)	774
MvaI	CCWGG	3 cut(s)	848, 1544, 1719
MvnI	CGCG	4 cut(s)	336, 338, 410, 599
MwoI	GCNNNNNNNGC	8 cut(s)	379, 407, 563, 752, 809, 1034, 1217, 1814
NaeI	GCCGGC	1 cut(s)	1703
NciI	CCSGG	1 cut(s)	1391
NcoI	CCATGG	2 cut(s)	612, 1224
NdeI	CATATG	2 cut(s)	793, 882
NdeII	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
NgoMIV	GCCGGC	1 cut(s)	1701
NheI	GCTAGC	1 cut(s)	1124
NlaIV	GGNNCC	4 cut(s)	1059, 1230, 1362, 1836
NmeAIII	GCCGAG	1 cut(s)	1192
NmuCI	GTSAC	4 cut(s)	202, 436, 1099, 1105
NsiI	ATGCAT	2 cut(s)	774, 997
NspI	RCATGY	3 cut(s)	405, 776, 1650
OliI	CACNNNNGTG	1 cut(s)	1331
PaeI	GCATGC	1 cut(s)	405
PaeR7I	CTCGAG	1 cut(s)	350
PagI	TCATGA	2 cut(s)	784, 1776
PciI	ACATGT	1 cut(s)	1646
PcsI	WCGNNNNNNNCGW	3 cut(s)	348, 414, 543
PctI	GAATGC	1 cut(s)	774
PdiI	GCCGGC	1 cut(s)	1703
PfeI	GAWTC	1 cut(s)	1687
PkrI	GCNGC	5 cut(s)	526, 580, 583, 603, 1036
PleI	GAGTC	6 cut(s)	176, 235, 986, 1505, 1529, 1559
PpsI	GAGTC	6 cut(s)	176, 235, 986, 1505, 1529, 1559
PpuMI	RGGWCCY	1 cut(s)	328
PscI	ACATGT	1 cut(s)	1646
PshBI	ATTAAT	1 cut(s)	960
Psp124BI	GAGCTC	2 cut(s)	384, 814
Psp5II	RGGWCCY	1 cut(s)	328
Psp6I	CCWGG	3 cut(s)	846, 1542, 1717
PspFI	CCCAGC	1 cut(s)	799
PspGI	CCWGG	3 cut(s)	846, 1542, 1717
PspN4I	GGNNCC	4 cut(s)	1059, 1230, 1362, 1836
PspPI	GGNCC	2 cut(s)	328, 1392
PspPPI	RGGWCCY	1 cut(s)	328
PspXI	VCTCGAGB	1 cut(s)	350
PstI	CTGCAG	2 cut(s)	522, 865
PstNI	CAGNNNCTG	1 cut(s)	1589
PsuI	RGATCY	3 cut(s)	393, 514, 1360
PvuII	CAGCTG	1 cut(s)	803
RsaI	GTAC	2 cut(s)	362, 1299
RsaNI	GTAC	2 cut(s)	361, 1298
RseI	CAYNNNNRTG	5 cut(s)	611, 824, 1095, 1331, 1781
SacI	GAGCTC	2 cut(s)	384, 814
SalI	GTCGAC	1 cut(s)	1508
SaqAI	TTAA	4 cut(s)	552, 960, 1853, 1899
SatI	GCNGC	5 cut(s)	525, 579, 582, 602, 1035
Sau3AI	GATC	9 cut(s)	288, 393, 414, 514, 787, 1360, 1398, 1655, 1711
Sau96I	GGNCC	2 cut(s)	328, 1392
SchI	GAGTC	6 cut(s)	176, 236, 987, 1505, 1529, 1559
ScrFI	CCNGG	4 cut(s)	848, 1391, 1544, 1719
SduI	GDGCHC	4 cut(s)	384, 591, 814, 1551
SfaNI	GCATC	6 cut(s)	530, 603, 628, 982, 1332, 1419
SfcI	CTRYAG	5 cut(s)	518, 861, 900, 1173, 1904
Sfr274I	CTCGAG	1 cut(s)	350
SinI	GGWCC	1 cut(s)	328
SlaI	CTCGAG	1 cut(s)	350
SmiMI	CAYNNNNRTG	5 cut(s)	611, 824, 1095, 1331, 1781
SmlI	CTYRAG	2 cut(s)	350, 1898
SmoI	CTYRAG	2 cut(s)	350, 1898
SphI	GCATGC	1 cut(s)	405
Sse9I	AATT	4 cut(s)	1155, 1301, 1571, 1860
SsiI	CCGC	6 cut(s)	222, 426, 524, 579, 582, 1209
SspI	AATATT	1 cut(s)	1744
SspMI	CTAG	8 cut(s)	200, 441, 618, 747, 1125, 1356, 1380, 1445
SstI	GAGCTC	2 cut(s)	384, 814
StyD4I	CCNGG	4 cut(s)	846, 1389, 1542, 1717
StyI	CCWWGG	2 cut(s)	612, 1224
TaaI	ACNGT	2 cut(s)	1105, 1507
TaiI	ACGT	4 cut(s)	175, 754, 929, 1674
TaqI	TCGA	5 cut(s)	351, 461, 843, 984, 1509
TasI	AATT	4 cut(s)	1155, 1301, 1571, 1860
TatI	WGTACW	2 cut(s)	360, 1297
TauI	GCSGC	3 cut(s)	527, 581, 584
TfiI	GAWTC	1 cut(s)	1687
Tru1I	TTAA	4 cut(s)	552, 960, 1853, 1899
Tru9I	TTAA	4 cut(s)	552, 960, 1853, 1899
TscAI	CASTG	1 cut(s)	865
TseFI	GTSAC	4 cut(s)	202, 436, 1099, 1105
TseI	GCWGC	2 cut(s)	601, 1034
Tsp45I	GTSAC	4 cut(s)	202, 436, 1099, 1105
TspDTI	ATGAA	7 cut(s)	63, 222, 662, 953, 1002, 1426, 1765
TspGWI	ACGGA	1 cut(s)	545
TspRI	CASTG	1 cut(s)	865
Vha464I	CTTAAG	1 cut(s)	1898
VneI	GTGCAC	1 cut(s)	1547
VpaK11BI	GGWCC	1 cut(s)	328
VspI	ATTAAT	1 cut(s)	960
XapI	RAATTY	1 cut(s)	1571
XceI	RCATGY	3 cut(s)	405, 776, 1650
XhoI	CTCGAG	1 cut(s)	350
XmiI	GTMKAC	2 cut(s)	1344, 1509
XspI	CTAG	8 cut(s)	200, 441, 618, 747, 1125, 1356, 1380, 1445
Zsp2I	ATGCAT	2 cut(s)	774, 997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.