Rw2G039120

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012863
Physical Location & Seq
Reverse (-)
64083819 .. 64088139
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G039120.1

Sequence Viewer

Length: 1911 bp
ATGGCTTCTTCTTCTTCTCTCAAAGCCATCTCCACCTTCACCACCCCTTCAAAAACACCACCACCTCCCTCATCATCATCATCAAAAGCTTCACTTCTCTCGTTCCTCAACAACACCTCCTCTCCAATCTCCCTAAAACTTTCTCACTCCATTCCTCGTTCACATTTTCGTGGAAACCATTCCGCTCTATCTCTCGCAGTCACCAATGCTTTGAACACCGTACAGTCTTCCTCTCCGGTGTCCACCTCCGGAGATCGAACAAAGTTGCCATCCTCCAACGCCAAGCCTACGATCCTGGTGTCCGAGAAACTTGGAGAGGCGGGCCTGGAGGTCCTACGCAAGTTCGGGAACCTGGAGTGCGCGTACAACCTCACGCCGGAGGAGCTCTGCGCCAAGATCTCAGCCTGCGACGCCCTGATCGTCCGGAGCGGGACGAAGGTCAACAGGCAGGTTTTCGAGGCGTCGAAGGGGAGGCTGAAGGTGGTGGGAAGGGCAGGGGTTGGAATAGACAATGTGGATCTGCAGGCGGCGACGGAGTTTGGATGCCTCGTCGTTAATGCGCCGACAGCAAACACCGTCGCTGCCGCAGAGCACGGGATCGCTCTGCTCACTTCCATGGCTAGAAATGTTGCTCAGGCAGATGCCTCCATGAAAGCTGGACTATGGAAACGCAACAAGTATGTCGGTGTCTCTCTTGTTGGGAAGACATTAGCAGTAATGGGATTTGGAAAAGTTGGATCAGAAGTTGCTAGACGTGCAAAAGGCTTGGGAATGCATGTTATAGCACATGATCCATATGCCCCAGCTGACAGAGCCCGTGCTGTTGGTGTGGATTTGGTCTCTTTCGACCAGGCCATCTCCACTGCAGATTTCGTGTCTCTGCATATGCCACTTACCCCTACTACATCAAAGGTGTTTAATGATGATACGTTTTCAAAGATGAAGAAGGGTGTTCGCATCATTAATGTTGCAAGGGGTGGAGTCATTGATGAAGATGCATTAGTGAGAGCCCTTGATAGCGGAGTTGTAGCTCAGGCTGCACTTGATGTGTTCACAGAGGAGCCTCCATCAAAAGAAAGCAAGTTAGTGCAACATGAGAATGTCACAGTCACACCTCATCTCGGAGCTAGTACAAAGGAAGCACAAGAAGGTGTAGCAATTGAAATAGCCGAGGCAGTAGTTGGAGCATTGCAAGGTGAACTTTCTGCAACCGCTGTCAATGCACCCATGGTTCCTCCTGAGGTTATGTTCGAGTTGTCTCCTTATGTTGTTCTTGCTGAGAAGCTAGGAAAGTTAGCTGTACAATTAGTAGCAGGAGGGAGTGGAATTACATCTGTGAGGGTGGTGTACAAATCAGCTCGAGATTCTGATGACTTGGATACTAGACTTCTCCGGGCTATGATCACTAAAGGCATCATTGAACCGATATCTACCTCATTCATCAATCTAGTAAATGCTGATTATGTTGCCAAGCAGAAAGGTCTGCGCATAAGTGAGGAAAAGGTATCTGTTGACTCATCTCCTGAGTTCCCTGTTGACTCAATCCAGGTGCACATATCCAATGTGGACTCCAAATTTGCAAGTTCAGTTTCTGATAATGGAAGCATAAGCATTGAGGGGAAAGTGAAATATGGGGAACCCCATCTCACATGTTTGGGTTCGTTTGGCGTGGACGTGAGCCTTGAAGGGAATCTTATTTTGTGCCGGCAGGTGGATCAACCCGGAATGATTGGCAAGGTGGGAAATATTCTTGGGAGCCAGAATGTGAATGTGAGCTTTATGAGTGTGGGAAGAACCATCCTGAAGAAAGCCATAATGGCTATTGGTGTAGATGAGGAACCAAACAAGGAGACTTTGAAGAAAATTGGGGATGTGGCTGCTATTGAAGAGTTTGTGTTCCTTAATCTATAG

Protein Analysis

636

Amino Acids

66.97

Weight (kDa)

7.64

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 98 - 407 2.2e-34 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 200 - 375 5.5e-63 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter PF19304 419 - 522 8.5e-14 D-3-phosphoglycerate dehydrogenase intervening domain
ACT PF01842 566 - 625 2.2e-09 ACT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012863)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17745 AT1G17745
fragaria_vesca FvH4_6g37460
malus_domestica MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica Prupe.3G168100_v2.0.a1
pyrus_communis pycom09g07670 pycom17g13710
rosa_chinensis RchiOBHm_Chr2g0150301
rosa_laevigata RLG00000020484
rosa_multiflora Rmu_sc0002335.1_g000001
rosa_roxburghii Rroxscaffold_2G00097310
rosa_rugosa Rorug02G0420400
rosa_samantha Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana Rw2G039120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1699
Acc16I TGCGCA 1 cut(s) 1487
Acc36I ACCTGC 2 cut(s) 439, 1699
AccBSI CCGCTC 2 cut(s) 185, 429
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 2 cut(s) 248, 423
AciI CCGC 7 cut(s) 183, 320, 429, 527, 585, 1020, 1212
AclWI GGATC 6 cut(s) 286, 525, 605, 745, 785, 1722
AcsI RAATTY 1 cut(s) 1574
AcuI CTGAAG 2 cut(s) 497, 1823
AcyI GRCGYC 2 cut(s) 411, 461
AfaI GTAC 5 cut(s) 222, 365, 1132, 1302, 1349
AfiI CCNNNNNNNGG 3 cut(s) 376, 1121, 1711
AflIII ACRYGT 1 cut(s) 1649
AgsI TTSAA 8 cut(s) 51, 214, 936, 1163, 1421, 1685, 1858, 1886
AjiI CACGTC 2 cut(s) 755, 1675
AjnI CCWGG 5 cut(s) 294, 324, 351, 849, 1545
Alw21I GWGCWC 3 cut(s) 387, 594, 1554
Alw26I GTCTC 5 cut(s) 694, 844, 882, 1263, 1844
Alw44I GTGCAC 1 cut(s) 1550
AlwI GGATC 6 cut(s) 286, 525, 605, 745, 785, 1722
AlwNI CAGNNNCTG 1 cut(s) 1592
Ama87I CYCGRG 1 cut(s) 1359
Aor13HI TCCGGA 2 cut(s) 248, 423
AoxI GGCC 2 cut(s) 322, 852
ApaLI GTGCAC 1 cut(s) 1550
ApeKI GCWGC 3 cut(s) 581, 1037, 1877
ApoI RAATTY 1 cut(s) 1574
ArsI GACNNNNNNTTYG 2 cut(s) 1559, 1591
AseI ATTAAT 1 cut(s) 963
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 4 cut(s) 362, 392, 562, 1488
AspS9I GGNCC 2 cut(s) 322, 331
AsuC2I CCSGG 2 cut(s) 1394, 1722
AsuHPI GGTGA 3 cut(s) 31, 193, 1208
AvaI CYCGRG 1 cut(s) 1359
AvaII GGWCC 1 cut(s) 331
AxyI CCTNAGG 1 cut(s) 1239
BaeGI GKGCMC 1 cut(s) 1554
BanII GRGCYC 3 cut(s) 387, 817, 1012
BbsI GAAGAC 2 cut(s) 219, 710
Bbv12I GWGCWC 3 cut(s) 387, 594, 1554
BbvI GCAGC 3 cut(s) 568, 1024, 1864
BccI CCATC 6 cut(s) 35, 277, 863, 1075, 1650, 1805
BciT130I CCWGG 5 cut(s) 296, 326, 353, 851, 1547
BciVI GTATCC 1 cut(s) 1372
BclI TGATCA 1 cut(s) 1401
BcnI CCSGG 2 cut(s) 1394, 1722
BcoDI GTCTC 5 cut(s) 694, 844, 882, 1263, 1844
BfaI CTAG 6 cut(s) 621, 750, 1128, 1286, 1383, 1448
BfmI CTRYAG 3 cut(s) 521, 864, 1907
BfuAI ACCTGC 2 cut(s) 439, 1699
BfuI GTATCC 1 cut(s) 1372
BglI GCCNNNNNGGC 1 cut(s) 1817
BglII AGATCT 1 cut(s) 396
BisI GCNGC 5 cut(s) 528, 582, 585, 1038, 1878
BlsI GCNGC 5 cut(s) 529, 583, 586, 1039, 1879
Bme1390I CCNGG 7 cut(s) 296, 326, 353, 851, 1394, 1547, 1722
Bme18I GGWCC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 1359
BmgBI CACGTC 2 cut(s) 755, 1675
BmgT120I GGNCC 2 cut(s) 322, 331
BmiI GGNNCC 6 cut(s) 350, 1062, 1233, 1638, 1757, 1839
BmrFI CCNGG 7 cut(s) 296, 326, 353, 851, 1394, 1547, 1722
BmsI GCATC 5 cut(s) 533, 631, 966, 985, 1422
BoxI GACNNNNGTC 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 219, 710
BpmI CTGGAG 2 cut(s) 347, 374
Bpu10I CCTNAGC 2 cut(s) 633, 1032
BpuMI CCSGG 2 cut(s) 1394, 1722
BsaHI GRCGYC 2 cut(s) 411, 461
BsaI GGTCTC 1 cut(s) 844
BsaJI CCNNGG 3 cut(s) 615, 1170, 1227
BsaWI WCCGGW 3 cut(s) 235, 248, 423
BsaXI ACNNNNNCTCC 6 cut(s) 101, 106, 131, 136, 347, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 1121, 1711
Bse118I RCCGGY 1 cut(s) 1704
Bse21I CCTNAGG 1 cut(s) 1239
Bse3DI GCAATG 1 cut(s) 1187
BseAI TCCGGA 2 cut(s) 248, 423
BseBI CCWGG 5 cut(s) 296, 326, 353, 851, 1547
BseDI CCNNGG 3 cut(s) 615, 1170, 1227
BseGI GGATG 4 cut(s) 269, 548, 1797, 1876
BseLI CCNNNNNNNGG 3 cut(s) 376, 1121, 1711
BseMI GCAATG 1 cut(s) 1187
BseMII CTCAG 6 cut(s) 414, 647, 1046, 1230, 1269, 1515
BseRI GAGGAG 3 cut(s) 109, 395, 1073
BseSI GKGCMC 1 cut(s) 1554
BseXI GCAGC 3 cut(s) 568, 1024, 1864
BseYI CCCAGC 1 cut(s) 802
BsgI GTGCAG 1 cut(s) 1023
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 2 cut(s) 324, 854
BsiHKAI GWGCWC 3 cut(s) 387, 594, 1554
BsiHKCI CYCGRG 1 cut(s) 1359
BsiSI CCGG 7 cut(s) 236, 249, 377, 424, 1393, 1705, 1722
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 3 cut(s) 376, 1121, 1711
BsmAI GTCTC 5 cut(s) 694, 844, 882, 1263, 1844
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 777
BsnI GGCC 2 cut(s) 324, 854
Bso31I GGTCTC 1 cut(s) 844
BsoBI CYCGRG 1 cut(s) 1359
Bsp1286I GDGCHC 5 cut(s) 387, 594, 817, 1012, 1554
Bsp13I TCCGGA 2 cut(s) 248, 423
Bsp1407I TGTACA 2 cut(s) 1300, 1347
Bsp19I CCATGG 2 cut(s) 615, 1227
BspACI CCGC 7 cut(s) 183, 320, 429, 527, 585, 1020, 1212
BspANI GGCC 2 cut(s) 324, 854
BspCNI CTCAG 6 cut(s) 413, 646, 1045, 1231, 1270, 1516
BspEI TCCGGA 2 cut(s) 248, 423
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 6 cut(s) 350, 1062, 1233, 1638, 1757, 1839
BspMAI CTGCAG 2 cut(s) 525, 868
BspMI ACCTGC 2 cut(s) 439, 1699
BspPI GGATC 6 cut(s) 286, 525, 605, 745, 785, 1722
BspTNI GGTCTC 1 cut(s) 844
BsrBI CCGCTC 2 cut(s) 185, 429
BsrDI GCAATG 1 cut(s) 1187
BsrFI RCCGGY 1 cut(s) 1704
BsrGI TGTACA 2 cut(s) 1300, 1347
BssAI RCCGGY 1 cut(s) 1704
BssECI CCNNGG 3 cut(s) 615, 1170, 1227
BssNI GRCGYC 2 cut(s) 411, 461
BssT1I CCWWGG 2 cut(s) 615, 1227
Bst2UI CCWGG 5 cut(s) 296, 326, 353, 851, 1547
Bst4CI ACNGT 4 cut(s) 220, 225, 577, 1108
Bst6I CTCTTC 1 cut(s) 1881
BstACI GRCGYC 2 cut(s) 411, 461
BstAUI TGTACA 2 cut(s) 1300, 1347
BstC8I GCNNGC 4 cut(s) 322, 406, 525, 1706
BstDEI CTNAG 6 cut(s) 400, 633, 1032, 1239, 1278, 1524
BstDSI CCRYGG 2 cut(s) 615, 1227
BstF5I GGATG 4 cut(s) 269, 548, 1797, 1876
BstFNI CGCG 1 cut(s) 362
BstHHI GCGC 4 cut(s) 362, 392, 562, 1488
BstMAI GTCTC 5 cut(s) 694, 844, 882, 1263, 1844
BstMWI GCNNNNNNNGC 8 cut(s) 382, 410, 566, 755, 812, 1037, 1220, 1817
BstNI CCWGG 5 cut(s) 296, 326, 353, 851, 1547
BstNSI RCATGY 2 cut(s) 779, 1653
BstPAI GACNNNNGTC 1 cut(s) 437
BstSCI CCNGG 7 cut(s) 294, 324, 351, 849, 1392, 1545, 1720
BstSFI CTRYAG 3 cut(s) 521, 864, 1907
BstSLI GKGCMC 1 cut(s) 1554
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 3 cut(s) 568, 1024, 1864
BstV2I GAAGAC 2 cut(s) 219, 710
BstX2I RGATCY 2 cut(s) 396, 517
BstYI RGATCY 2 cut(s) 396, 517
Bsu36I CCTNAGG 1 cut(s) 1239
BsuI GTATCC 1 cut(s) 1372
BsuRI GGCC 2 cut(s) 324, 854
BtgI CCRYGG 2 cut(s) 615, 1227
BtrI CACGTC 2 cut(s) 755, 1675
BtsCI GGATG 4 cut(s) 269, 548, 1797, 1876
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 1 cut(s) 861
BveI ACCTGC 2 cut(s) 439, 1699
Cac8I GCNNGC 4 cut(s) 322, 406, 525, 1706
CaiI CAGNNNCTG 1 cut(s) 1592
CfoI GCGC 4 cut(s) 362, 392, 562, 1488
Cfr10I RCCGGY 1 cut(s) 1704
Cfr13I GGNCC 2 cut(s) 322, 331
CseI GACGC 2 cut(s) 419, 450
Csp6I GTAC 5 cut(s) 221, 364, 1131, 1301, 1348
CviAII CATG 7 cut(s) 616, 649, 776, 788, 1094, 1228, 1650
CviQI GTAC 5 cut(s) 221, 364, 1131, 1301, 1348
DdeI CTNAG 6 cut(s) 400, 633, 1032, 1239, 1278, 1524
Eam1104I CTCTTC 1 cut(s) 1881
EarI CTCTTC 1 cut(s) 1881
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 2 cut(s) 615, 1227
Eco24I GRGCYC 3 cut(s) 387, 817, 1012
Eco31I GGTCTC 1 cut(s) 844
Eco32I GATATC 1 cut(s) 1428
Eco47I GGWCC 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 2 cut(s) 497, 1823
Eco81I CCTNAGG 1 cut(s) 1239
Eco88I CYCGRG 1 cut(s) 1359
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 5 cut(s) 294, 324, 351, 849, 1545
EcoRV GATATC 1 cut(s) 1428
EcoT14I CCWWGG 2 cut(s) 615, 1227
EcoT22I ATGCAT 2 cut(s) 777, 1000
EcoT38I GRGCYC 3 cut(s) 387, 817, 1012
ErhI CCWWGG 2 cut(s) 615, 1227
FaeI CATG 7 cut(s) 619, 652, 779, 791, 1097, 1231, 1653
FalI AAGNNNNNCTT 6 cut(s) 78, 110, 1185, 1217, 1677, 1709
FaqI GGGAC 1 cut(s) 445
FatI CATG 7 cut(s) 615, 648, 775, 787, 1093, 1227, 1649
FauI CCCGC 2 cut(s) 313, 422
FauNDI CATATG 2 cut(s) 796, 885
FbaI TGATCA 1 cut(s) 1401
Fnu4HI GCNGC 5 cut(s) 528, 582, 585, 1038, 1878
FokI GGATG 4 cut(s) 256, 555, 1784, 1883
FriOI GRGCYC 3 cut(s) 387, 817, 1012
Fsp4HI GCNGC 5 cut(s) 528, 582, 585, 1038, 1878
FspBI CTAG 6 cut(s) 621, 750, 1128, 1286, 1383, 1448
FspI TGCGCA 1 cut(s) 1487
GlaI GCGC 4 cut(s) 361, 391, 561, 1487
GluI GCNGC 5 cut(s) 528, 582, 585, 1038, 1878
GsaI CCCAGC 1 cut(s) 806
GsuI CTGGAG 2 cut(s) 347, 374
HaeIII GGCC 2 cut(s) 324, 854
HapII CCGG 7 cut(s) 236, 249, 377, 424, 1393, 1705, 1722
HgaI GACGC 2 cut(s) 419, 450
HhaI GCGC 4 cut(s) 362, 392, 562, 1488
Hin1I GRCGYC 2 cut(s) 411, 461
Hin1II CATG 7 cut(s) 619, 652, 779, 791, 1097, 1231, 1653
Hin6I GCGC 4 cut(s) 360, 390, 560, 1486
HinP1I GCGC 4 cut(s) 360, 390, 560, 1486
HincII GTYRAC 3 cut(s) 442, 1513, 1537
HindII GTYRAC 3 cut(s) 442, 1513, 1537
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 6 cut(s) 981, 1364, 1514, 1538, 1568, 1690
HpaII CCGG 7 cut(s) 236, 249, 377, 424, 1393, 1705, 1722
HphI GGTGA 3 cut(s) 31, 193, 1208
Hpy188I TCNGA 5 cut(s) 304, 742, 1124, 1369, 1594
Hpy188III TCNNGA 7 cut(s) 249, 346, 424, 1238, 1361, 1523, 1801
Hpy99I CGWCG 5 cut(s) 413, 466, 535, 554, 581
HpyAV CCTTC 9 cut(s) 46, 57, 430, 460, 472, 483, 940, 1142, 1679
HpyCH4III ACNGT 4 cut(s) 220, 225, 577, 1108
HpyCH4IV ACGT 3 cut(s) 754, 929, 1674
HpyF10VI GCNNNNNNNGC 8 cut(s) 382, 410, 566, 755, 812, 1037, 1220, 1817
HpyF3I CTNAG 6 cut(s) 400, 633, 1032, 1239, 1278, 1524
HpySE526I ACGT 3 cut(s) 754, 929, 1674
Hsp92I GRCGYC 2 cut(s) 411, 461
Hsp92II CATG 7 cut(s) 619, 652, 779, 791, 1097, 1231, 1653
HspAI GCGC 4 cut(s) 360, 390, 560, 1486
Kpn2I TCCGGA 2 cut(s) 248, 423
KroI GCCGGC 1 cut(s) 1704
KroNI GCCGGC 1 cut(s) 1706
Ksp22I TGATCA 1 cut(s) 1401
LmnI GCTCC 6 cut(s) 382, 426, 1060, 1124, 1184, 1755
Lsp1109I GCAGC 3 cut(s) 568, 1024, 1864
LweI GCATC 5 cut(s) 533, 631, 966, 985, 1422
MaeI CTAG 6 cut(s) 621, 750, 1128, 1286, 1383, 1448
MaeII ACGT 3 cut(s) 754, 929, 1674
MaeIII GTNAC 3 cut(s) 199, 1102, 1108
MbiI CCGCTC 2 cut(s) 185, 429
MfeI CAATTG 1 cut(s) 1158
MflI RGATCY 2 cut(s) 396, 517
MhlI GDGCHC 5 cut(s) 387, 594, 817, 1012, 1554
MluCI AATT 5 cut(s) 1158, 1304, 1326, 1574, 1863
MlyI GAGTC 4 cut(s) 990, 1508, 1532, 1562
MmeI TCCRAC 4 cut(s) 300, 481, 715, 1162
Mph1103I ATGCAT 2 cut(s) 777, 1000
MroI TCCGGA 2 cut(s) 248, 423
MroNI GCCGGC 1 cut(s) 1704
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 4 cut(s) 555, 918, 963, 1902
MslI CAYNNNNRTG 3 cut(s) 168, 614, 1098
MspA1I CMGCKG 2 cut(s) 806, 1214
MspI CCGG 7 cut(s) 236, 249, 377, 424, 1393, 1705, 1722
MspR9I CCNGG 7 cut(s) 296, 326, 353, 851, 1394, 1547, 1722
MunI CAATTG 1 cut(s) 1158
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 5 cut(s) 296, 326, 353, 851, 1547
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 8 cut(s) 382, 410, 566, 755, 812, 1037, 1220, 1817
NaeI GCCGGC 1 cut(s) 1706
NciI CCSGG 2 cut(s) 1394, 1722
NcoI CCATGG 2 cut(s) 615, 1227
NdeI CATATG 2 cut(s) 796, 885
NgoMIV GCCGGC 1 cut(s) 1704
NlaIII CATG 7 cut(s) 619, 652, 779, 791, 1097, 1231, 1653
NlaIV GGNNCC 6 cut(s) 350, 1062, 1233, 1638, 1757, 1839
NmeAIII GCCGAG 1 cut(s) 1195
NmuCI GTSAC 3 cut(s) 199, 1102, 1108
NsbI TGCGCA 1 cut(s) 1487
NsiI ATGCAT 2 cut(s) 777, 1000
NspI RCATGY 2 cut(s) 779, 1653
PaeR7I CTCGAG 1 cut(s) 1359
PaqCI CACCTGC 1 cut(s) 1699
PciI ACATGT 1 cut(s) 1649
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 777
PdiI GCCGGC 1 cut(s) 1706
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 1364, 1690
PkrI GCNGC 5 cut(s) 529, 583, 586, 1039, 1879
PleI GAGTC 4 cut(s) 989, 1508, 1532, 1562
PpsI GAGTC 4 cut(s) 989, 1508, 1532, 1562
PpuMI RGGWCCY 1 cut(s) 331
PscI ACATGT 1 cut(s) 1649
PshAI GACNNNNGTC 1 cut(s) 437
PshBI ATTAAT 1 cut(s) 963
Psp124BI GAGCTC 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 5 cut(s) 294, 324, 351, 849, 1545
PspFI CCCAGC 1 cut(s) 802
PspGI CCWGG 5 cut(s) 294, 324, 351, 849, 1545
PspN4I GGNNCC 6 cut(s) 350, 1062, 1233, 1638, 1757, 1839
PspPI GGNCC 2 cut(s) 322, 331
PspPPI RGGWCCY 1 cut(s) 331
PstI CTGCAG 2 cut(s) 525, 868
PstNI CAGNNNCTG 1 cut(s) 1592
PsuI RGATCY 2 cut(s) 396, 517
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 5 cut(s) 222, 365, 1132, 1302, 1349
RsaNI GTAC 5 cut(s) 221, 364, 1131, 1301, 1348
RseI CAYNNNNRTG 3 cut(s) 168, 614, 1098
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 4 cut(s) 555, 918, 963, 1902
SatI GCNGC 5 cut(s) 528, 582, 585, 1038, 1878
Sau96I GGNCC 2 cut(s) 322, 331
SchI GAGTC 4 cut(s) 990, 1508, 1532, 1562
ScrFI CCNGG 7 cut(s) 296, 326, 353, 851, 1394, 1547, 1722
SduI GDGCHC 5 cut(s) 387, 594, 817, 1012, 1554
SfaNI GCATC 5 cut(s) 533, 631, 966, 985, 1422
SfcI CTRYAG 3 cut(s) 521, 864, 1907
Sfr274I CTCGAG 1 cut(s) 1359
SinI GGWCC 1 cut(s) 331
SlaI CTCGAG 1 cut(s) 1359
SmiMI CAYNNNNRTG 3 cut(s) 168, 614, 1098
SmlI CTYRAG 1 cut(s) 1359
SmoI CTYRAG 1 cut(s) 1359
Sse9I AATT 5 cut(s) 1158, 1304, 1326, 1574, 1863
SsiI CCGC 7 cut(s) 183, 320, 429, 527, 585, 1020, 1212
SspI AATATT 1 cut(s) 1747
SspMI CTAG 6 cut(s) 621, 750, 1128, 1286, 1383, 1448
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 7 cut(s) 294, 324, 351, 849, 1392, 1545, 1720
StyI CCWWGG 2 cut(s) 615, 1227
TaaI ACNGT 4 cut(s) 220, 225, 577, 1108
TaiI ACGT 3 cut(s) 757, 932, 1677
TaqI TCGA 6 cut(s) 256, 456, 464, 846, 1251, 1360
TasI AATT 5 cut(s) 1158, 1304, 1326, 1574, 1863
TatI WGTACW 3 cut(s) 1130, 1300, 1347
TauI GCSGC 2 cut(s) 530, 587
TfiI GAWTC 2 cut(s) 1364, 1690
Tru1I TTAA 4 cut(s) 555, 918, 963, 1902
Tru9I TTAA 4 cut(s) 555, 918, 963, 1902
TscAI CASTG 1 cut(s) 868
TseFI GTSAC 3 cut(s) 199, 1102, 1108
TseI GCWGC 3 cut(s) 581, 1037, 1877
Tsp45I GTSAC 3 cut(s) 199, 1102, 1108
TspDTI ATGAA 4 cut(s) 665, 956, 1005, 1429
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 1 cut(s) 868
VneI GTGCAC 1 cut(s) 1550
VpaK11BI GGWCC 1 cut(s) 331
VspI ATTAAT 1 cut(s) 963
XapI RAATTY 1 cut(s) 1574
XceI RCATGY 2 cut(s) 779, 1653
XhoI CTCGAG 1 cut(s) 1359
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 6 cut(s) 621, 750, 1128, 1286, 1383, 1448
Zsp2I ATGCAT 2 cut(s) 777, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.