Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012863

Physical Location & Seq

Genomic Coordinates Forward (+)

12984378 .. 12989035

Gene Structure 4658 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1143900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGGCTTCCTCCTGCATCAAGGTCATCTCCTCCTCCGCCTTCACCGCCTCCCCTCCGCCGTCTCAAACCAAACCACAACCGTCCATACCCTACCTTCTTTCCTTCCTCCACGCCACCTCCCGCCCCATCTCCTTAAAACTCTCCCGTTCTCGATCCCATAATTCGCATTCCCATTCTTTTCTCATAACCAACGTTCTAAAAAAAGCCGAGTCATCATCCGACACATATCTTTCCAAGTCCTCCCAGCTTCCCAAGCCAACGTCCTCGGATTCAACTCCGACCGTCCTGGTCACCGAAAAACTCGGAGAAGCCGGTCTTGAAGTCCTACGTGGCTTTGGCAACTTGGAGTGCGCGTACAACCTCTCCCCTGAGGAGCTCTGCGCCAAGATCTCGACATGCGACGCTTTGATTGTTCGGAGCGGCACGAAGGTGACCAGGCAGGTGTTTGAGGCCGCCAAAGGGAGGTTGAAGGTGGTGGGAAGAGCTGGTGTGGGAATAGACAATGTGGATCTGCAGGCGGCCACGGAGTTTGGATGTCTGGTCGTTAATGCGCCCACGGCAAACACCGTGGCTGCAGCAGAGCACGGGATAGCTCTGCTCACTGCCATGTCACGAAATGTTGCACAAGCTGATGCTTCCATCAAAGCTGGCAAATGGCAACGAAACAAGTACGTTGGCGTCTCTTTGGTTGGGAAGACATTGGCAGTAATGGGATTTGGAAAAGTTGGATCCGAAGTTGCTAGACGTGCGAAAGGCTTGGGAATGCACGTTATTTCGCATGATCCTTATGCCCCGGTTGACAGAGCACGCGCTGTTGGTGTGGAATTGGTCTCTTTTGAGCAGGCAATTGGCACTGCAGATTTCATCTCACTGCATATGCCTCTGACTCCTACAACATCAAAGGTGTTGAATGACAAAACATTCGCGCAGATGAAGAAGGGAGTCCGAATCATTAATGTTGCCCGGGGTGGAGTCATTGATGAGGATGCACTAGTGAGAGCTCTTGATAGTGGAATTGTTGCTCAGGCTGCACTTGATGTGTTCGCAGAGGAGCCTCCATCCAAAGATAGCAAGCTAGTGCAACACGAGAATGTCACAGTCACACCTCATCTAGGAGCCAGCACAAAGGAAGCGCAGGAAGGTGTTGCCATTGAAATAGCCGAGGCTGTAGTTGGAGCATTGAAAGGTGAACTTTCTGCAACCGCTGTCAACGCTCCGATGGTCCCTCCTGAGGTTCTGGCTGAGTTGTCTCCTTATGTAGTGCTAGCCGAGAAGCTGGGGAAGTTGGCTGTACAATTAGTAGCAGGAGGGAGTGGGATCACATCTGTGAGGGTGGTTTACAAAACAGCTCGCGACCCCGATGACTTGGATACCAGGCTTCTCAGGGCCATGATCACTAAAGGCATCATCGAACCAATATCTGCCTCGTTTATCAATCTAGTCAACGCAGATTTCGTTGCAAAGGATAAAGGCCTACGCATAACCGAGGAAAGGGTAGCTATAGACTCATCCCCTGAGTTCCCCGTTGCTTCAATCCAGGTACACATTACCAATGTGGAGTCCAAATTCGCAAGTTGTGTTTCCAAAAATGGAAACATAAGCATTGAGGGGAAAGTAAAATATGGAAAGCCCCATCTGACATTGTTGGGATCGTTTGGCGTGGACGTGAGCCTCGAGGGCAATCTCATTCTGTGCCGGCAGGTGGATCAGCCAGGCATGATTGGGCAGGTGGGGAAAATACTTGGGACTGAGAATGTGAATGTGAACTTTATGAGTGTGGGAAGAACTATTCAGAAGAAGGCCATAATGGCGATTGGAGTGGATGAGGAACCGAAGGAGGAGTTGCTAAATAAAATCGGAAAGGTGCCTGATATCGAAGAGTTCGTGTTCCTTAAGCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

634

Amino Acids

67.14

Weight (kDa)

8.47

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	95 - 404	1e-34	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	197 - 372	1.2e-62	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter	PF19304	416 - 523	1.5e-13	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	563 - 623	5.8e-09	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17745 AT1G17745
fragaria_vesca	FvH4_6g37460
malus_domestica	MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica	Prupe.3G168100_v2.0.a1
pyrus_communis	pycom09g07670 pycom17g13710
rosa_chinensis	RchiOBHm_Chr2g0150301
rosa_laevigata	RLG00000020484
rosa_multiflora	Rmu_sc0002335.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097310
rosa_rugosa	Rorug02G0420400
rosa_samantha	Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana	Rw2G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	430, 1690, 1717
AbsI	CCTCGAGG	1 cut(s)	1673
Acc36I	ACCTGC	3 cut(s)	430, 1690, 1717
AccB1I	GGYRCC	1 cut(s)	1864
AccBSI	CCGCTC	1 cut(s)	420
AccII	CGCG	4 cut(s)	353, 810, 926, 1353
AciI	CCGC	8 cut(s)	36, 45, 56, 121, 420, 453, 518, 1203
AclI	AACGTT	1 cut(s)	192
AclWI	GGATC	8 cut(s)	147, 516, 723, 736, 776, 1325, 1657, 1713
AcoI	YGGCCR	1 cut(s)	519
AcsI	RAATTY	1 cut(s)	1565
AcyI	GRCGYC	1 cut(s)	678
AfaI	GTAC	4 cut(s)	356, 671, 1293, 1542
AfiI	CCNNNNNNNGG	6 cut(s)	459, 462, 1112, 1231, 1491, 1702
AflII	CTTAAG	1 cut(s)	1892
AgsI	TTSAA	7 cut(s)	273, 320, 469, 910, 1154, 1183, 1533
AhlI	ACTAGT	1 cut(s)	991
AjiI	CACGTC	2 cut(s)	746, 1666
AjnI	CCWGG	5 cut(s)	285, 434, 1373, 1536, 1711
AleI	CACNNNNGTG	2 cut(s)	428, 1325
Alw21I	GWGCWC	4 cut(s)	378, 585, 808, 1003
Alw26I	GTCTC	4 cut(s)	66, 685, 835, 1254
AlwI	GGATC	8 cut(s)	147, 516, 723, 736, 776, 1325, 1657, 1713
Ama87I	CYCGRG	2 cut(s)	963, 1673
AoxI	GGCC	5 cut(s)	450, 519, 1386, 1471, 1800
ApeKI	GCWGC	3 cut(s)	572, 575, 1028
ApoI	RAATTY	1 cut(s)	1565
ArsI	GACNNNNNNTTYG	2 cut(s)	905, 937
AseI	ATTAAT	1 cut(s)	954
AspLEI	GCGC	6 cut(s)	353, 383, 553, 812, 928, 1135
AspS9I	GGNCC	2 cut(s)	1222, 1386
AsuC2I	CCSGG	3 cut(s)	794, 964, 965
AsuHPI	GGTGA	4 cut(s)	34, 283, 442, 1199
AsuNHI	GCTAGC	1 cut(s)	1264
AvaI	CYCGRG	2 cut(s)	963, 1673
AvaII	GGWCC	1 cut(s)	1222
AxyI	CCTNAGG	2 cut(s)	369, 1230
BamHI	GGATCC	1 cut(s)	728
BanI	GGYRCC	1 cut(s)	1864
BanII	GRGCYC	2 cut(s)	378, 1003
BauI	CACGAG	1 cut(s)	1085
BbsI	GAAGAC	1 cut(s)	701
Bbv12I	GWGCWC	4 cut(s)	378, 585, 808, 1003
BbvI	GCAGC	3 cut(s)	559, 587, 1015
BccI	CCATC	5 cut(s)	134, 647, 1066, 1213, 1641
BceAI	ACGGC	2 cut(s)	43, 573
BciT130I	CCWGG	5 cut(s)	287, 436, 1375, 1538, 1713
BciVI	GTATCC	1 cut(s)	1363
BclI	TGATCA	1 cut(s)	1392
BcnI	CCSGG	3 cut(s)	794, 964, 965
BcoDI	GTCTC	4 cut(s)	66, 685, 835, 1254
BcuI	ACTAGT	1 cut(s)	991
BfaI	CTAG	6 cut(s)	741, 992, 1076, 1112, 1265, 1439
BfmI	CTRYAG	6 cut(s)	512, 573, 855, 1167, 1500, 1898
BfrI	CTTAAG	1 cut(s)	1892
BfuAI	ACCTGC	3 cut(s)	430, 1690, 1717
BfuI	GTATCC	1 cut(s)	1363
BglI	GCCNNNNNGGC	2 cut(s)	1677, 1808
BglII	AGATCT	1 cut(s)	387
BisI	GCNGC	6 cut(s)	421, 453, 519, 573, 576, 1029
BlsI	GCNGC	6 cut(s)	422, 454, 520, 574, 577, 1030
Bme1390I	CCNGG	8 cut(s)	287, 436, 794, 964, 965, 1375, 1538, 1713
Bme18I	GGWCC	1 cut(s)	1222
BmeT110I	CYCGRG	2 cut(s)	963, 1673
BmgBI	CACGTC	2 cut(s)	746, 1666
BmgT120I	GGNCC	2 cut(s)	1222, 1386
BmiI	GGNNCC	6 cut(s)	730, 1053, 1117, 1224, 1830, 1866
BmrFI	CCNGG	8 cut(s)	287, 436, 794, 964, 965, 1375, 1538, 1713
BmsI	GCATC	4 cut(s)	24, 622, 976, 1413
BmtI	GCTAGC	1 cut(s)	1268
BpiI	GAAGAC	1 cut(s)	701
Bpu10I	CCTNAGC	1 cut(s)	1023
BpuMI	CCSGG	3 cut(s)	794, 964, 965
BsaAI	YACGTR	1 cut(s)	329
BsaHI	GRCGYC	1 cut(s)	678
BsaI	GGTCTC	1 cut(s)	835
BsaJI	CCNNGG	9 cut(s)	264, 522, 555, 567, 792, 963, 964, 1161, 1485
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 338, 347, 368, 377
Bsc4I	CCNNNNNNNGG	6 cut(s)	459, 462, 1112, 1231, 1491, 1702
Bse118I	RCCGGY	2 cut(s)	311, 1695
Bse21I	CCTNAGG	2 cut(s)	369, 1230
BseBI	CCWGG	5 cut(s)	287, 436, 1375, 1538, 1713
BseDI	CCNNGG	9 cut(s)	264, 522, 555, 567, 792, 963, 964, 1161, 1485
BseGI	GGATG	6 cut(s)	215, 539, 991, 1058, 1508, 1828
BseLI	CCNNNNNNNGG	6 cut(s)	459, 462, 1112, 1231, 1491, 1702
BseMII	CTCAG	7 cut(s)	360, 1037, 1221, 1233, 1396, 1506, 1740
BseRI	GAGGAG	5 cut(s)	19, 22, 386, 1064, 1853
BseXI	GCAGC	3 cut(s)	559, 587, 1015
BseYI	CCCAGC	2 cut(s)	243, 1276
BsgI	GTGCAG	1 cut(s)	1014
Bsh1236I	CGCG	4 cut(s)	353, 810, 926, 1353
Bsh1285I	CGRYCG	1 cut(s)	282
BshFI	GGCC	5 cut(s)	452, 521, 1388, 1473, 1802
BshNI	GGYRCC	1 cut(s)	1864
BsiEI	CGRYCG	1 cut(s)	282
BsiHKAI	GWGCWC	4 cut(s)	378, 585, 808, 1003
BsiHKCI	CYCGRG	2 cut(s)	963, 1673
BsiSI	CCGG	4 cut(s)	312, 794, 964, 1696
BslFI	GGGAC	2 cut(s)	1208, 1759
BslI	CCNNNNNNNGG	6 cut(s)	459, 462, 1112, 1231, 1491, 1702
BsmAI	GTCTC	4 cut(s)	66, 685, 835, 1254
BsmBI	CGTCTC	2 cut(s)	66, 685
BsmFI	GGGAC	2 cut(s)	1208, 1759
BsmI	GAATGC	2 cut(s)	166, 768
BsnI	GGCC	5 cut(s)	452, 521, 1388, 1473, 1802
Bso31I	GGTCTC	1 cut(s)	835
BsoBI	CYCGRG	2 cut(s)	963, 1673
Bsp1286I	GDGCHC	4 cut(s)	378, 585, 808, 1003
Bsp1407I	TGTACA	1 cut(s)	1291
Bsp143I	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
Bsp68I	TCGCGA	1 cut(s)	1353
BspACI	CCGC	8 cut(s)	36, 45, 56, 121, 420, 453, 518, 1203
BspANI	GGCC	5 cut(s)	452, 521, 1388, 1473, 1802
BspCNI	CTCAG	7 cut(s)	361, 1036, 1222, 1234, 1395, 1507, 1741
BspFNI	CGCG	4 cut(s)	353, 810, 926, 1353
BspLI	GGNNCC	6 cut(s)	730, 1053, 1117, 1224, 1830, 1866
BspMAI	CTGCAG	3 cut(s)	516, 577, 859
BspMI	ACCTGC	3 cut(s)	430, 1690, 1717
BspOI	GCTAGC	1 cut(s)	1268
BspPI	GGATC	8 cut(s)	147, 516, 723, 736, 776, 1325, 1657, 1713
BspQI	GCTCTTC	1 cut(s)	475
BspT107I	GGYRCC	1 cut(s)	1864
BspTI	CTTAAG	1 cut(s)	1892
BspTNI	GGTCTC	1 cut(s)	835
BsrBI	CCGCTC	1 cut(s)	420
BsrFI	RCCGGY	2 cut(s)	311, 1695
BsrGI	TGTACA	1 cut(s)	1291
BssAI	RCCGGY	2 cut(s)	311, 1695
BssECI	CCNNGG	9 cut(s)	264, 522, 555, 567, 792, 963, 964, 1161, 1485
BssMI	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
BssNI	GRCGYC	1 cut(s)	678
BssSI	CACGAG	1 cut(s)	1085
Bst2BI	CACGAG	1 cut(s)	1085
Bst2UI	CCWGG	5 cut(s)	287, 436, 1375, 1538, 1713
Bst4CI	ACNGT	4 cut(s)	81, 283, 568, 1099
Bst6I	CTCTTC	2 cut(s)	475, 1872
BstACI	GRCGYC	1 cut(s)	678
BstAFI	CTTAAG	1 cut(s)	1892
BstAUI	TGTACA	1 cut(s)	1291
BstBAI	YACGTR	1 cut(s)	329
BstC8I	GCNNGC	9 cut(s)	516, 649, 808, 843, 1073, 1120, 1266, 1351, 1697
BstDEI	CTNAG	7 cut(s)	369, 1023, 1230, 1242, 1382, 1515, 1749
BstDSI	CCRYGG	3 cut(s)	522, 555, 567
BstEII	GGTNACC	2 cut(s)	289, 430
BstENI	CCTNNNNNAGG	1 cut(s)	1110
BstF5I	GGATG	6 cut(s)	215, 539, 991, 1058, 1508, 1828
BstFNI	CGCG	4 cut(s)	353, 810, 926, 1353
BstHHI	GCGC	6 cut(s)	353, 383, 553, 812, 928, 1135
BstKTI	GATC	9 cut(s)	155, 390, 511, 731, 784, 1320, 1395, 1652, 1708
BstMAI	GTCTC	4 cut(s)	66, 685, 835, 1254
BstMBI	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
BstMCI	CGRYCG	1 cut(s)	282
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 253, 557, 746, 1028, 1211, 1677, 1808
BstNI	CCWGG	5 cut(s)	287, 436, 1375, 1538, 1713
BstNSI	RCATGY	1 cut(s)	399
BstPI	GGTNACC	2 cut(s)	289, 430
BstSCI	CCNGG	8 cut(s)	285, 434, 792, 962, 963, 1373, 1536, 1711
BstSFI	CTRYAG	6 cut(s)	512, 573, 855, 1167, 1500, 1898
BstUI	CGCG	4 cut(s)	353, 810, 926, 1353
BstV1I	GCAGC	3 cut(s)	559, 587, 1015
BstV2I	GAAGAC	1 cut(s)	701
BstX2I	RGATCY	3 cut(s)	387, 508, 728
BstYI	RGATCY	3 cut(s)	387, 508, 728
Bsu36I	CCTNAGG	2 cut(s)	369, 1230
BsuI	GTATCC	1 cut(s)	1363
BsuRI	GGCC	5 cut(s)	452, 521, 1388, 1473, 1802
BtgI	CCRYGG	3 cut(s)	522, 555, 567
BtrI	CACGTC	2 cut(s)	746, 1666
BtsCI	GGATG	6 cut(s)	215, 539, 991, 1058, 1508, 1828
BtsI	GCAGTG	3 cut(s)	600, 852, 869
BtsIMutI	CAGTG	3 cut(s)	600, 852, 869
BtuMI	TCGCGA	1 cut(s)	1353
BveI	ACCTGC	3 cut(s)	430, 1690, 1717
Cac8I	GCNNGC	9 cut(s)	516, 649, 808, 843, 1073, 1120, 1266, 1351, 1697
CfoI	GCGC	6 cut(s)	353, 383, 553, 812, 928, 1135
Cfr10I	RCCGGY	2 cut(s)	311, 1695
Cfr13I	GGNCC	2 cut(s)	1222, 1386
Cfr9I	CCCGGG	1 cut(s)	963
CseI	GACGC	2 cut(s)	410, 667
Csp6I	GTAC	4 cut(s)	355, 670, 1292, 1541
CspCI	CAANNNNNGTGG	4 cut(s)	511, 546, 549, 584
CviAII	CATG	5 cut(s)	396, 607, 779, 1390, 1717
CviQI	GTAC	4 cut(s)	355, 670, 1292, 1541
DdeI	CTNAG	7 cut(s)	369, 1023, 1230, 1242, 1382, 1515, 1749
DpnI	GATC	9 cut(s)	154, 389, 510, 730, 783, 1319, 1394, 1651, 1707
DpnII	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
EaeI	YGGCCR	1 cut(s)	519
Eam1104I	CTCTTC	2 cut(s)	475, 1872
EarI	CTCTTC	2 cut(s)	475, 1872
EciI	GGCGGA	2 cut(s)	25, 45
Ecl136II	GAGCTC	2 cut(s)	376, 1001
Eco147I	AGGCCT	1 cut(s)	1473
Eco24I	GRGCYC	2 cut(s)	378, 1003
Eco31I	GGTCTC	1 cut(s)	835
Eco32I	GATATC	1 cut(s)	1873
Eco47I	GGWCC	1 cut(s)	1222
Eco53kI	GAGCTC	2 cut(s)	376, 1001
Eco81I	CCTNAGG	2 cut(s)	369, 1230
Eco88I	CYCGRG	2 cut(s)	963, 1673
Eco91I	GGTNACC	2 cut(s)	289, 430
EcoICRI	GAGCTC	2 cut(s)	376, 1001
EcoNI	CCTNNNNNAGG	1 cut(s)	1110
EcoO65I	GGTNACC	2 cut(s)	289, 430
EcoRII	CCWGG	5 cut(s)	285, 434, 1373, 1536, 1711
EcoRV	GATATC	1 cut(s)	1873
EcoT38I	GRGCYC	2 cut(s)	378, 1003
Esp3I	CGTCTC	2 cut(s)	66, 685
FaeI	CATG	5 cut(s)	399, 610, 782, 1393, 1720
FalI	AAGNNNNNCTT	4 cut(s)	300, 332, 1176, 1208
FaqI	GGGAC	2 cut(s)	1208, 1759
FatI	CATG	5 cut(s)	395, 606, 778, 1389, 1716
FauI	CCCGC	1 cut(s)	128
FauNDI	CATATG	1 cut(s)	876
FbaI	TGATCA	1 cut(s)	1392
Fnu4HI	GCNGC	6 cut(s)	421, 453, 519, 573, 576, 1029
FokI	GGATG	6 cut(s)	202, 546, 998, 1045, 1495, 1835
FriOI	GRGCYC	2 cut(s)	378, 1003
Fsp4HI	GCNGC	6 cut(s)	421, 453, 519, 573, 576, 1029
FspBI	CTAG	6 cut(s)	741, 992, 1076, 1112, 1265, 1439
GlaI	GCGC	6 cut(s)	352, 382, 552, 811, 927, 1134
GluI	GCNGC	6 cut(s)	421, 453, 519, 573, 576, 1029
GsaI	CCCAGC	2 cut(s)	247, 1280
HaeIII	GGCC	5 cut(s)	452, 521, 1388, 1473, 1802
HapII	CCGG	4 cut(s)	312, 794, 964, 1696
HgaI	GACGC	2 cut(s)	410, 667
HhaI	GCGC	6 cut(s)	353, 383, 553, 812, 928, 1135
Hin1I	GRCGYC	1 cut(s)	678
Hin1II	CATG	5 cut(s)	399, 610, 782, 1393, 1720
Hin6I	GCGC	6 cut(s)	351, 381, 551, 810, 926, 1133
HinP1I	GCGC	6 cut(s)	351, 381, 551, 810, 926, 1133
HincII	GTYRAC	3 cut(s)	799, 1210, 1444
HindII	GTYRAC	3 cut(s)	799, 1210, 1444
HinfI	GANTC	8 cut(s)	209, 269, 886, 942, 948, 972, 1505, 1559
HpaII	CCGG	4 cut(s)	312, 794, 964, 1696
HphI	GGTGA	4 cut(s)	34, 283, 442, 1199
Hpy166II	GTNNAC	8 cut(s)	799, 1190, 1210, 1339, 1444, 1543, 1663, 1765
Hpy188III	TCNNGA	7 cut(s)	150, 317, 391, 612, 1004, 1229, 1352
Hpy8I	GTNNAC	8 cut(s)	799, 1190, 1210, 1339, 1444, 1543, 1663, 1765
Hpy99I	CGWCG	1 cut(s)	404
HpyAV	CCTTC	9 cut(s)	49, 104, 112, 421, 463, 931, 1133, 1792, 1828
HpyCH4III	ACNGT	4 cut(s)	81, 283, 568, 1099
HpyCH4IV	ACGT	7 cut(s)	192, 260, 328, 672, 745, 768, 1665
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 253, 557, 746, 1028, 1211, 1677, 1808
HpyF3I	CTNAG	7 cut(s)	369, 1023, 1230, 1242, 1382, 1515, 1749
HpySE526I	ACGT	7 cut(s)	192, 260, 328, 672, 745, 768, 1665
Hsp92I	GRCGYC	1 cut(s)	678
Hsp92II	CATG	5 cut(s)	399, 610, 782, 1393, 1720
HspAI	GCGC	6 cut(s)	351, 381, 551, 810, 926, 1133
KroI	GCCGGC	1 cut(s)	1695
KroNI	GCCGGC	1 cut(s)	1697
Ksp22I	TGATCA	1 cut(s)	1392
Kzo9I	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
LguI	GCTCTTC	1 cut(s)	475
LmnI	GCTCC	6 cut(s)	373, 417, 1051, 1115, 1175, 1219
Lsp1109I	GCAGC	3 cut(s)	559, 587, 1015
LweI	GCATC	4 cut(s)	24, 622, 976, 1413
MaeI	CTAG	6 cut(s)	741, 992, 1076, 1112, 1265, 1439
MaeII	ACGT	7 cut(s)	192, 260, 328, 672, 745, 768, 1665
MaeIII	GTNAC	5 cut(s)	289, 430, 609, 1093, 1099
MalI	GATC	9 cut(s)	154, 389, 510, 730, 783, 1319, 1394, 1651, 1707
MbiI	CCGCTC	1 cut(s)	420
MboI	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
MboII	GAAGA	6 cut(s)	492, 706, 946, 1794, 1807, 1889
MfeI	CAATTG	1 cut(s)	846
MflI	RGATCY	3 cut(s)	387, 508, 728
MhlI	GDGCHC	4 cut(s)	378, 585, 808, 1003
MluCI	AATT	6 cut(s)	160, 824, 846, 1014, 1295, 1565
MlyI	GAGTC	6 cut(s)	218, 880, 951, 981, 1499, 1568
MmeI	TCCRAC	4 cut(s)	243, 302, 706, 1153
MroNI	GCCGGC	1 cut(s)	1695
MseI	TTAA	4 cut(s)	134, 546, 954, 1893
MslI	CAYNNNNRTG	4 cut(s)	428, 605, 1089, 1325
MspA1I	CMGCKG	1 cut(s)	1205
MspCI	CTTAAG	1 cut(s)	1892
MspI	CCGG	4 cut(s)	312, 794, 964, 1696
MspR9I	CCNGG	8 cut(s)	287, 436, 794, 964, 965, 1375, 1538, 1713
MunI	CAATTG	1 cut(s)	846
Mva1269I	GAATGC	2 cut(s)	166, 768
MvaI	CCWGG	5 cut(s)	287, 436, 1375, 1538, 1713
MvnI	CGCG	4 cut(s)	353, 810, 926, 1353
MwoI	GCNNNNNNNGC	8 cut(s)	44, 253, 557, 746, 1028, 1211, 1677, 1808
NaeI	GCCGGC	1 cut(s)	1697
NciI	CCSGG	3 cut(s)	794, 964, 965
NdeI	CATATG	1 cut(s)	876
NdeII	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
NgoMIV	GCCGGC	1 cut(s)	1695
NheI	GCTAGC	1 cut(s)	1264
NlaIII	CATG	5 cut(s)	399, 610, 782, 1393, 1720
NlaIV	GGNNCC	6 cut(s)	730, 1053, 1117, 1224, 1830, 1866
NmeAIII	GCCGAG	3 cut(s)	232, 1186, 1294
NmuCI	GTSAC	5 cut(s)	289, 430, 609, 1093, 1099
NruI	TCGCGA	1 cut(s)	1353
NspI	RCATGY	1 cut(s)	399
OliI	CACNNNNGTG	2 cut(s)	428, 1325
PaeR7I	CTCGAG	1 cut(s)	1673
PaqCI	CACCTGC	3 cut(s)	430, 1690, 1717
PceI	AGGCCT	1 cut(s)	1473
PciSI	GCTCTTC	1 cut(s)	475
PcsI	WCGNNNNNNNCGW	3 cut(s)	1452, 1671, 1881
PctI	GAATGC	2 cut(s)	166, 768
PdiI	GCCGGC	1 cut(s)	1697
PfeI	GAWTC	2 cut(s)	269, 948
PkrI	GCNGC	6 cut(s)	422, 454, 520, 574, 577, 1030
PleI	GAGTC	6 cut(s)	217, 880, 950, 980, 1499, 1567
PpsI	GAGTC	6 cut(s)	217, 880, 950, 980, 1499, 1567
Ppu21I	YACGTR	1 cut(s)	329
PshBI	ATTAAT	1 cut(s)	954
Psp124BI	GAGCTC	2 cut(s)	378, 1003
Psp1406I	AACGTT	1 cut(s)	192
Psp6I	CCWGG	5 cut(s)	285, 434, 1373, 1536, 1711
PspEI	GGTNACC	2 cut(s)	289, 430
PspFI	CCCAGC	2 cut(s)	243, 1276
PspGI	CCWGG	5 cut(s)	285, 434, 1373, 1536, 1711
PspN4I	GGNNCC	6 cut(s)	730, 1053, 1117, 1224, 1830, 1866
PspPI	GGNCC	2 cut(s)	1222, 1386
PspXI	VCTCGAGB	1 cut(s)	1673
PstI	CTGCAG	3 cut(s)	516, 577, 859
PsuI	RGATCY	3 cut(s)	387, 508, 728
RruI	TCGCGA	1 cut(s)	1353
RsaI	GTAC	4 cut(s)	356, 671, 1293, 1542
RsaNI	GTAC	4 cut(s)	355, 670, 1292, 1541
RseI	CAYNNNNRTG	4 cut(s)	428, 605, 1089, 1325
SacI	GAGCTC	2 cut(s)	378, 1003
SapI	GCTCTTC	1 cut(s)	475
SaqAI	TTAA	4 cut(s)	134, 546, 954, 1893
SatI	GCNGC	6 cut(s)	421, 453, 519, 573, 576, 1029
Sau3AI	GATC	9 cut(s)	152, 387, 508, 728, 781, 1317, 1392, 1649, 1705
Sau96I	GGNCC	2 cut(s)	1222, 1386
SchI	GAGTC	6 cut(s)	218, 880, 951, 981, 1499, 1568
ScrFI	CCNGG	8 cut(s)	287, 436, 794, 964, 965, 1375, 1538, 1713
SduI	GDGCHC	4 cut(s)	378, 585, 808, 1003
SfaNI	GCATC	4 cut(s)	24, 622, 976, 1413
SfcI	CTRYAG	6 cut(s)	512, 573, 855, 1167, 1500, 1898
Sfr274I	CTCGAG	1 cut(s)	1673
SinI	GGWCC	1 cut(s)	1222
SlaI	CTCGAG	1 cut(s)	1673
SmaI	CCCGGG	1 cut(s)	965
SmiMI	CAYNNNNRTG	4 cut(s)	428, 605, 1089, 1325
SmlI	CTYRAG	2 cut(s)	1673, 1892
SmoI	CTYRAG	2 cut(s)	1673, 1892
SpeI	ACTAGT	1 cut(s)	991
Sse9I	AATT	6 cut(s)	160, 824, 846, 1014, 1295, 1565
SseBI	AGGCCT	1 cut(s)	1473
SsiI	CCGC	8 cut(s)	36, 45, 56, 121, 420, 453, 518, 1203
SspMI	CTAG	6 cut(s)	741, 992, 1076, 1112, 1265, 1439
SstI	GAGCTC	2 cut(s)	378, 1003
StuI	AGGCCT	1 cut(s)	1473
StyD4I	CCNGG	8 cut(s)	285, 434, 792, 962, 963, 1373, 1536, 1711
TaaI	ACNGT	4 cut(s)	81, 283, 568, 1099
TaiI	ACGT	7 cut(s)	195, 263, 331, 675, 748, 771, 1668
TaqI	TCGA	5 cut(s)	151, 392, 1410, 1674, 1875
TasI	AATT	6 cut(s)	160, 824, 846, 1014, 1295, 1565
TatI	WGTACW	1 cut(s)	1291
TauI	GCSGC	3 cut(s)	423, 455, 521
TfiI	GAWTC	2 cut(s)	269, 948
Tru1I	TTAA	4 cut(s)	134, 546, 954, 1893
Tru9I	TTAA	4 cut(s)	134, 546, 954, 1893
TscAI	CASTG	3 cut(s)	607, 859, 876
TseFI	GTSAC	5 cut(s)	289, 430, 609, 1093, 1099
TseI	GCWGC	3 cut(s)	572, 575, 1028
Tsp45I	GTSAC	5 cut(s)	289, 430, 609, 1093, 1099
TspDTI	ATGAA	2 cut(s)	853, 947
TspGWI	ACGGA	1 cut(s)	539
TspMI	CCCGGG	1 cut(s)	963
TspRI	CASTG	3 cut(s)	607, 859, 876
Vha464I	CTTAAG	1 cut(s)	1892
VpaK11BI	GGWCC	1 cut(s)	1222
VspI	ATTAAT	1 cut(s)	954
XagI	CCTNNNNNAGG	1 cut(s)	1110
XapI	RAATTY	1 cut(s)	1565
XceI	RCATGY	1 cut(s)	399
XhoI	CTCGAG	1 cut(s)	1673
XmaI	CCCGGG	1 cut(s)	963
XspI	CTAG	6 cut(s)	741, 992, 1076, 1112, 1265, 1439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.