Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0012863

Physical Location & Seq

Genomic Coordinates Reverse (-)

65511082 .. 65514925

Gene Structure 3844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020484

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGGCTTCTTCTTCTCTCAAAGCCATCTCCACCTTCACCACCCCTTCAAAAACACCACCACGTCCCTCATCATCATCATCATCATCATCATCAAAAGCTTCACTTCTCTCCTTCCTCAACAACACCTCCTCTCCAATCTCCCTAAAACTTTCTCACTCCATTCCTCGTTCACATTTTCGTGGAAACCATTCCACTCTATCTCTCGCAGTCACCAATGCTTTGAACACCGTACAGTCTTCCTCTCCGGTGTCCACCTCCGGAGATCGAACAAAGTTGCCCTCCTCCGTCGCCAAGCCTACGATCCTGGTCTCCGAGAAACTTGGAGAGGCGGGCCTGGAGGTCCTACGCAAGTTCGGGAACCTGGAGTGCGCGTACAACCTCTCGCCTGAGGAGCTCTGCGCCAAGATCTCAGCCTGCGACGCCCTGATCGTCCGGAGCGGGACGAAGGTCAACAGGCAGGTTTTCGAGGCGTCGAAGGGGAGGCTGAAGGTGGTGGGAAGGGCAGGGGTTGGAATAGACAATGTGGATCTGCAGGCGGCGACCGAGTTTGGATGCCTCGTCGTTAATGCGCCGACGGCAAACACCGTCGCCGCCGCAGAGCACGGGATCGCTCTGCTCACTTCCATGGCTAGAAATGTTGCTCAGGCAGATGCCTCCATGAAAGCTGGACTGTGGAAACGCAACAAGTATGTCGGTGTCTCTCTTGTTGGGAAGACATTAGCAGTAATGGGATTTGGAAAAGTTGGATCAGAAGTTGCTAGACGTGCAAAAGGCTTGGGAATGCATGTTATAGCACATGATCCATATGCCCCAGCTGACAGAGCCCGTGCCGTTGGTGTGGATTTGGTCTCTTTCGACCAGGCCATCTCCACTGCAGATTTCGTGTCTCTGCATATGCCACTTACCCCTACTACATCAAAGGTGTTTAATGATGATACGTTTTCAAAGATGAAGAAGGGTGTTCGCATCATTAATGTTGCAAGGGGTGGAGTCATTGATGAAGATGCATTAGTGAGAGCCCTTGATAGCGGAGTTGTGGCTCAGGCTGCACTTGATGTGTTCACAGAGGAGCCTCCATCAAAAGAAAGCAAGTTAGTGCAACACGAGAATGTCACAGTCACACCTCATCTCGGAGCTAGCACAAAGGAAGCACAAGAAGGTGTAGCAATTGAAATAGCCGAGGCAGTAGTTGGAGCATTGCAAGGTGAACTTTCTGCAACTGCTGTCAATGCACCCATGGTTCCTCCTGAGGTTATGTTAGAGTTGTCTCCTTATGTTGTTCTTGCTGAGAAGCTGGGAAAGTTAGCTGTACAATTAGTAGCAGGAGGGAGTGGAATTACATCTGTGAGGGTGGTGTACAAATCAGCTCGAGATTCTGATGACTTGGATACTAGACTTCTCCGGGCTATGATCACTAAAGGCATCATTGAACCGATATCTACCTCATTCATCAATCTAGTAAATGCTGATTATGTTGCCAAGCAGAAAGGTCTGCGCATAAGTGAGGAAAAGGTATCTATTGACTCATCTCCTGAGTTCCCTGTTGACTCAATCCAGGTGCACATATCCAATGTGGACTCCAAATTTGCAAGTTCAGTTTCTGATAATGGAAGCATAAGCATTGAGGGGAAAGTGAAATATGGGGAACCCCATCTCACATGTTTGGGTTCGTTTGGCGTGGACGTGAGCCTTGAAGGGAACCTTATTTTGTGCCGGCAGGTGGATCAACCCGGAATGATTGGCAAGGTGGGAAATATTCTTGGGAGCCAGAATGTGAATGTGAGCTTTATGAGTGTGGGAAGAACCATCCTGAAGAAAGCCATAATGGCTATTGGTGTAGATGAGGAACCAAACAAGGAAACTCTGAAGAAAATTGGGGATGTGGCTGCTATTGAAGAGTTTGTGTTCCTTAAGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

640

Amino Acids

67.29

Weight (kDa)

8.33

Isoelectric Point (pI)

39.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	101 - 410	1.8e-34	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	203 - 378	5.6e-63	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter	PF19304	422 - 524	1.1e-13	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	569 - 628	2.3e-09	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17745 AT1G17745
fragaria_vesca	FvH4_6g37460
malus_domestica	MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica	Prupe.3G168100_v2.0.a1
pyrus_communis	pycom09g07670 pycom17g13710
rosa_chinensis	RchiOBHm_Chr2g0150301
rosa_laevigata	RLG00000020484
rosa_multiflora	Rmu_sc0002335.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097310
rosa_rugosa	Rorug02G0420400
rosa_samantha	Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana	Rw2G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1708
Acc16I	TGCGCA	1 cut(s)	1496
Acc36I	ACCTGC	2 cut(s)	448, 1708
AccBSI	CCGCTC	1 cut(s)	438
AccII	CGCG	1 cut(s)	371
AccIII	TCCGGA	2 cut(s)	257, 432
AciI	CCGC	6 cut(s)	329, 438, 536, 591, 594, 1029
AclWI	GGATC	6 cut(s)	295, 534, 614, 754, 794, 1731
AcsI	RAATTY	1 cut(s)	1583
AcuI	CTGAAG	3 cut(s)	506, 1832, 1886
AcyI	GRCGYC	2 cut(s)	420, 470
AfaI	GTAC	4 cut(s)	231, 374, 1311, 1358
AfiI	CCNNNNNNNGG	2 cut(s)	1130, 1720
AflII	CTTAAG	1 cut(s)	1910
AflIII	ACRYGT	1 cut(s)	1658
AgsI	TTSAA	7 cut(s)	48, 223, 945, 1172, 1430, 1694, 1895
AjiI	CACGTC	3 cut(s)	62, 764, 1684
AjnI	CCWGG	5 cut(s)	303, 333, 360, 858, 1554
Alw21I	GWGCWC	3 cut(s)	396, 603, 1563
Alw26I	GTCTC	5 cut(s)	313, 703, 853, 891, 1272
Alw44I	GTGCAC	1 cut(s)	1559
AlwI	GGATC	6 cut(s)	295, 534, 614, 754, 794, 1731
AlwNI	CAGNNNCTG	1 cut(s)	1601
Ama87I	CYCGRG	1 cut(s)	1368
Aor13HI	TCCGGA	2 cut(s)	257, 432
AoxI	GGCC	2 cut(s)	331, 861
ApaLI	GTGCAC	1 cut(s)	1559
ApeKI	GCWGC	2 cut(s)	1046, 1886
ApoI	RAATTY	1 cut(s)	1583
ArsI	GACNNNNNNTTYG	2 cut(s)	1568, 1600
AseI	ATTAAT	1 cut(s)	972
Asp700I	GAANNNNTTC	1 cut(s)	187
AspLEI	GCGC	4 cut(s)	371, 401, 571, 1497
AspS9I	GGNCC	2 cut(s)	331, 340
AsuC2I	CCSGG	2 cut(s)	1403, 1731
AsuHPI	GGTGA	3 cut(s)	28, 202, 1217
AsuNHI	GCTAGC	1 cut(s)	1136
AvaI	CYCGRG	1 cut(s)	1368
AvaII	GGWCC	1 cut(s)	340
AxyI	CCTNAGG	2 cut(s)	387, 1248
BaeGI	GKGCMC	1 cut(s)	1563
BanII	GRGCYC	3 cut(s)	396, 826, 1021
BauI	CACGAG	1 cut(s)	1103
BbsI	GAAGAC	2 cut(s)	228, 719
Bbv12I	GWGCWC	3 cut(s)	396, 603, 1563
BbvI	GCAGC	2 cut(s)	1033, 1873
BccI	CCATC	5 cut(s)	32, 872, 1084, 1659, 1814
BceAI	ACGGC	2 cut(s)	591, 815
BciT130I	CCWGG	5 cut(s)	305, 335, 362, 860, 1556
BciVI	GTATCC	1 cut(s)	1381
BclI	TGATCA	1 cut(s)	1410
BcnI	CCSGG	2 cut(s)	1403, 1731
BcoDI	GTCTC	5 cut(s)	313, 703, 853, 891, 1272
BfaI	CTAG	5 cut(s)	630, 759, 1137, 1392, 1457
BfmI	CTRYAG	2 cut(s)	530, 873
BfrI	CTTAAG	1 cut(s)	1910
BfuAI	ACCTGC	2 cut(s)	448, 1708
BfuI	GTATCC	1 cut(s)	1381
BglI	GCCNNNNNGGC	1 cut(s)	1826
BglII	AGATCT	1 cut(s)	405
BisI	GCNGC	5 cut(s)	537, 591, 594, 1047, 1887
BlsI	GCNGC	5 cut(s)	538, 592, 595, 1048, 1888
Bme1390I	CCNGG	7 cut(s)	305, 335, 362, 860, 1403, 1556, 1731
Bme18I	GGWCC	1 cut(s)	340
BmeT110I	CYCGRG	1 cut(s)	1368
BmgBI	CACGTC	3 cut(s)	62, 764, 1684
BmgT120I	GGNCC	2 cut(s)	331, 340
BmiI	GGNNCC	7 cut(s)	359, 1071, 1242, 1647, 1700, 1766, 1848
BmrFI	CCNGG	7 cut(s)	305, 335, 362, 860, 1403, 1556, 1731
BmsI	GCATC	5 cut(s)	542, 640, 975, 994, 1431
BmtI	GCTAGC	1 cut(s)	1140
BoxI	GACNNNNGTC	1 cut(s)	446
BpiI	GAAGAC	2 cut(s)	228, 719
BpmI	CTGGAG	2 cut(s)	356, 383
Bpu10I	CCTNAGC	2 cut(s)	642, 1041
BpuMI	CCSGG	2 cut(s)	1403, 1731
BsaHI	GRCGYC	2 cut(s)	420, 470
BsaI	GGTCTC	2 cut(s)	313, 853
BsaJI	CCNNGG	3 cut(s)	624, 1179, 1236
BsaWI	WCCGGW	3 cut(s)	244, 257, 432
BsaXI	ACNNNNNCTCC	6 cut(s)	110, 115, 140, 145, 356, 386
Bsc4I	CCNNNNNNNGG	2 cut(s)	1130, 1720
Bse118I	RCCGGY	1 cut(s)	1713
Bse21I	CCTNAGG	2 cut(s)	387, 1248
Bse3DI	GCAATG	1 cut(s)	1196
BseAI	TCCGGA	2 cut(s)	257, 432
BseBI	CCWGG	5 cut(s)	305, 335, 362, 860, 1556
BseDI	CCNNGG	3 cut(s)	624, 1179, 1236
BseGI	GGATG	3 cut(s)	557, 1806, 1885
BseLI	CCNNNNNNNGG	2 cut(s)	1130, 1720
BseMI	GCAATG	1 cut(s)	1196
BseMII	CTCAG	7 cut(s)	378, 423, 656, 1055, 1239, 1278, 1524
BseRI	GAGGAG	4 cut(s)	118, 271, 404, 1082
BseSI	GKGCMC	1 cut(s)	1563
BseXI	GCAGC	2 cut(s)	1033, 1873
BseYI	CCCAGC	2 cut(s)	811, 1294
BsgI	GTGCAG	1 cut(s)	1032
Bsh1236I	CGCG	1 cut(s)	371
Bsh1285I	CGRYCG	1 cut(s)	543
BshFI	GGCC	2 cut(s)	333, 863
BsiEI	CGRYCG	1 cut(s)	543
BsiHKAI	GWGCWC	3 cut(s)	396, 603, 1563
BsiHKCI	CYCGRG	1 cut(s)	1368
BsiSI	CCGG	6 cut(s)	245, 258, 433, 1402, 1714, 1731
BslFI	GGGAC	2 cut(s)	48, 454
BslI	CCNNNNNNNGG	2 cut(s)	1130, 1720
BsmAI	GTCTC	5 cut(s)	313, 703, 853, 891, 1272
BsmFI	GGGAC	2 cut(s)	48, 454
BsmI	GAATGC	1 cut(s)	786
BsnI	GGCC	2 cut(s)	333, 863
Bso31I	GGTCTC	2 cut(s)	313, 853
BsoBI	CYCGRG	1 cut(s)	1368
Bsp1286I	GDGCHC	5 cut(s)	396, 603, 826, 1021, 1563
Bsp13I	TCCGGA	2 cut(s)	257, 432
Bsp1407I	TGTACA	2 cut(s)	1309, 1356
Bsp19I	CCATGG	2 cut(s)	624, 1236
BspACI	CCGC	6 cut(s)	329, 438, 536, 591, 594, 1029
BspANI	GGCC	2 cut(s)	333, 863
BspCNI	CTCAG	7 cut(s)	379, 422, 655, 1054, 1240, 1279, 1525
BspEI	TCCGGA	2 cut(s)	257, 432
BspFNI	CGCG	1 cut(s)	371
BspLI	GGNNCC	7 cut(s)	359, 1071, 1242, 1647, 1700, 1766, 1848
BspMAI	CTGCAG	2 cut(s)	534, 877
BspMI	ACCTGC	2 cut(s)	448, 1708
BspOI	GCTAGC	1 cut(s)	1140
BspPI	GGATC	6 cut(s)	295, 534, 614, 754, 794, 1731
BspTI	CTTAAG	1 cut(s)	1910
BspTNI	GGTCTC	2 cut(s)	313, 853
BsrBI	CCGCTC	1 cut(s)	438
BsrDI	GCAATG	1 cut(s)	1196
BsrFI	RCCGGY	1 cut(s)	1713
BsrGI	TGTACA	2 cut(s)	1309, 1356
BssAI	RCCGGY	1 cut(s)	1713
BssECI	CCNNGG	3 cut(s)	624, 1179, 1236
BssNI	GRCGYC	2 cut(s)	420, 470
BssSI	CACGAG	1 cut(s)	1103
BssT1I	CCWWGG	2 cut(s)	624, 1236
Bst2BI	CACGAG	1 cut(s)	1103
Bst2UI	CCWGG	5 cut(s)	305, 335, 362, 860, 1556
Bst4CI	ACNGT	5 cut(s)	229, 234, 586, 672, 1117
Bst6I	CTCTTC	1 cut(s)	1890
BstACI	GRCGYC	2 cut(s)	420, 470
BstAFI	CTTAAG	1 cut(s)	1910
BstAUI	TGTACA	2 cut(s)	1309, 1356
BstC8I	GCNNGC	5 cut(s)	331, 415, 534, 1138, 1715
BstDEI	CTNAG	7 cut(s)	387, 409, 642, 1041, 1248, 1287, 1533
BstDSI	CCRYGG	2 cut(s)	624, 1236
BstF5I	GGATG	3 cut(s)	557, 1806, 1885
BstFNI	CGCG	1 cut(s)	371
BstHHI	GCGC	4 cut(s)	371, 401, 571, 1497
BstMAI	GTCTC	5 cut(s)	313, 703, 853, 891, 1272
BstMCI	CGRYCG	1 cut(s)	543
BstMWI	GCNNNNNNNGC	8 cut(s)	391, 419, 575, 764, 821, 1046, 1229, 1826
BstNI	CCWGG	5 cut(s)	305, 335, 362, 860, 1556
BstNSI	RCATGY	2 cut(s)	788, 1662
BstPAI	GACNNNNGTC	1 cut(s)	446
BstSCI	CCNGG	7 cut(s)	303, 333, 360, 858, 1401, 1554, 1729
BstSFI	CTRYAG	2 cut(s)	530, 873
BstSLI	GKGCMC	1 cut(s)	1563
BstUI	CGCG	1 cut(s)	371
BstV1I	GCAGC	2 cut(s)	1033, 1873
BstV2I	GAAGAC	2 cut(s)	228, 719
BstX2I	RGATCY	2 cut(s)	405, 526
BstYI	RGATCY	2 cut(s)	405, 526
Bsu36I	CCTNAGG	2 cut(s)	387, 1248
BsuI	GTATCC	1 cut(s)	1381
BsuRI	GGCC	2 cut(s)	333, 863
BtgI	CCRYGG	2 cut(s)	624, 1236
BtrI	CACGTC	3 cut(s)	62, 764, 1684
BtsCI	GGATG	3 cut(s)	557, 1806, 1885
BtsI	GCAGTG	1 cut(s)	870
BtsIMutI	CAGTG	1 cut(s)	870
BveI	ACCTGC	2 cut(s)	448, 1708
Cac8I	GCNNGC	5 cut(s)	331, 415, 534, 1138, 1715
CaiI	CAGNNNCTG	1 cut(s)	1601
CfoI	GCGC	4 cut(s)	371, 401, 571, 1497
Cfr10I	RCCGGY	1 cut(s)	1713
Cfr13I	GGNCC	2 cut(s)	331, 340
CseI	GACGC	2 cut(s)	428, 459
Csp6I	GTAC	4 cut(s)	230, 373, 1310, 1357
CviAII	CATG	6 cut(s)	625, 658, 785, 797, 1237, 1659
CviQI	GTAC	4 cut(s)	230, 373, 1310, 1357
DdeI	CTNAG	7 cut(s)	387, 409, 642, 1041, 1248, 1287, 1533
Eam1104I	CTCTTC	1 cut(s)	1890
EarI	CTCTTC	1 cut(s)	1890
Ecl136II	GAGCTC	1 cut(s)	394
Eco130I	CCWWGG	2 cut(s)	624, 1236
Eco24I	GRGCYC	3 cut(s)	396, 826, 1021
Eco31I	GGTCTC	2 cut(s)	313, 853
Eco32I	GATATC	1 cut(s)	1437
Eco47I	GGWCC	1 cut(s)	340
Eco53kI	GAGCTC	1 cut(s)	394
Eco57I	CTGAAG	3 cut(s)	506, 1832, 1886
Eco81I	CCTNAGG	2 cut(s)	387, 1248
Eco88I	CYCGRG	1 cut(s)	1368
EcoICRI	GAGCTC	1 cut(s)	394
EcoO109I	RGGNCCY	1 cut(s)	340
EcoRII	CCWGG	5 cut(s)	303, 333, 360, 858, 1554
EcoRV	GATATC	1 cut(s)	1437
EcoT14I	CCWWGG	2 cut(s)	624, 1236
EcoT22I	ATGCAT	2 cut(s)	786, 1009
EcoT38I	GRGCYC	3 cut(s)	396, 826, 1021
ErhI	CCWWGG	2 cut(s)	624, 1236
FaeI	CATG	6 cut(s)	628, 661, 788, 800, 1240, 1662
FalI	AAGNNNNNCTT	6 cut(s)	87, 119, 1194, 1226, 1686, 1718
FaqI	GGGAC	2 cut(s)	48, 454
FatI	CATG	6 cut(s)	624, 657, 784, 796, 1236, 1658
FauI	CCCGC	2 cut(s)	322, 431
FauNDI	CATATG	2 cut(s)	805, 894
FbaI	TGATCA	1 cut(s)	1410
Fnu4HI	GCNGC	5 cut(s)	537, 591, 594, 1047, 1887
FokI	GGATG	3 cut(s)	564, 1793, 1892
FriOI	GRGCYC	3 cut(s)	396, 826, 1021
Fsp4HI	GCNGC	5 cut(s)	537, 591, 594, 1047, 1887
FspBI	CTAG	5 cut(s)	630, 759, 1137, 1392, 1457
FspI	TGCGCA	1 cut(s)	1496
GlaI	GCGC	4 cut(s)	370, 400, 570, 1496
GluI	GCNGC	5 cut(s)	537, 591, 594, 1047, 1887
GsaI	CCCAGC	2 cut(s)	815, 1298
GsuI	CTGGAG	2 cut(s)	356, 383
HaeIII	GGCC	2 cut(s)	333, 863
HapII	CCGG	6 cut(s)	245, 258, 433, 1402, 1714, 1731
HgaI	GACGC	2 cut(s)	428, 459
HhaI	GCGC	4 cut(s)	371, 401, 571, 1497
Hin1I	GRCGYC	2 cut(s)	420, 470
Hin1II	CATG	6 cut(s)	628, 661, 788, 800, 1240, 1662
Hin6I	GCGC	4 cut(s)	369, 399, 569, 1495
HinP1I	GCGC	4 cut(s)	369, 399, 569, 1495
HincII	GTYRAC	2 cut(s)	451, 1546
HindII	GTYRAC	2 cut(s)	451, 1546
HindIII	AAGCTT	1 cut(s)	96
HinfI	GANTC	5 cut(s)	990, 1373, 1523, 1547, 1577
HpaII	CCGG	6 cut(s)	245, 258, 433, 1402, 1714, 1731
HphI	GGTGA	3 cut(s)	28, 202, 1217
Hpy188I	TCNGA	6 cut(s)	313, 751, 1133, 1378, 1603, 1866
Hpy188III	TCNNGA	7 cut(s)	258, 355, 433, 1247, 1370, 1532, 1810
Hpy99I	CGWCG	6 cut(s)	290, 422, 475, 563, 577, 590
HpyCH4III	ACNGT	5 cut(s)	229, 234, 586, 672, 1117
HpyCH4IV	ACGT	4 cut(s)	61, 763, 938, 1683
HpyF10VI	GCNNNNNNNGC	8 cut(s)	391, 419, 575, 764, 821, 1046, 1229, 1826
HpyF3I	CTNAG	7 cut(s)	387, 409, 642, 1041, 1248, 1287, 1533
HpySE526I	ACGT	4 cut(s)	61, 763, 938, 1683
Hsp92I	GRCGYC	2 cut(s)	420, 470
Hsp92II	CATG	6 cut(s)	628, 661, 788, 800, 1240, 1662
HspAI	GCGC	4 cut(s)	369, 399, 569, 1495
Kpn2I	TCCGGA	2 cut(s)	257, 432
KroI	GCCGGC	1 cut(s)	1713
KroNI	GCCGGC	1 cut(s)	1715
Ksp22I	TGATCA	1 cut(s)	1410
LmnI	GCTCC	6 cut(s)	391, 435, 1069, 1133, 1193, 1764
Lsp1109I	GCAGC	2 cut(s)	1033, 1873
LweI	GCATC	5 cut(s)	542, 640, 975, 994, 1431
MaeI	CTAG	5 cut(s)	630, 759, 1137, 1392, 1457
MaeII	ACGT	4 cut(s)	61, 763, 938, 1683
MaeIII	GTNAC	3 cut(s)	208, 1111, 1117
MbiI	CCGCTC	1 cut(s)	438
MboII	GAAGA	9 cut(s)	3, 228, 724, 964, 1013, 1812, 1825, 1879, 1907
MfeI	CAATTG	1 cut(s)	1167
MflI	RGATCY	2 cut(s)	405, 526
MhlI	GDGCHC	5 cut(s)	396, 603, 826, 1021, 1563
MluCI	AATT	5 cut(s)	1167, 1313, 1335, 1583, 1872
MlyI	GAGTC	4 cut(s)	999, 1517, 1541, 1571
MmeI	TCCRAC	3 cut(s)	490, 724, 1171
Mph1103I	ATGCAT	2 cut(s)	786, 1009
MroI	TCCGGA	2 cut(s)	257, 432
MroNI	GCCGGC	1 cut(s)	1713
MroXI	GAANNNNTTC	1 cut(s)	187
MseI	TTAA	4 cut(s)	564, 927, 972, 1911
MslI	CAYNNNNRTG	3 cut(s)	177, 623, 1107
MspA1I	CMGCKG	1 cut(s)	815
MspCI	CTTAAG	1 cut(s)	1910
MspI	CCGG	6 cut(s)	245, 258, 433, 1402, 1714, 1731
MspR9I	CCNGG	7 cut(s)	305, 335, 362, 860, 1403, 1556, 1731
MunI	CAATTG	1 cut(s)	1167
Mva1269I	GAATGC	1 cut(s)	786
MvaI	CCWGG	5 cut(s)	305, 335, 362, 860, 1556
MvnI	CGCG	1 cut(s)	371
MwoI	GCNNNNNNNGC	8 cut(s)	391, 419, 575, 764, 821, 1046, 1229, 1826
NaeI	GCCGGC	1 cut(s)	1715
NciI	CCSGG	2 cut(s)	1403, 1731
NcoI	CCATGG	2 cut(s)	624, 1236
NdeI	CATATG	2 cut(s)	805, 894
NgoMIV	GCCGGC	1 cut(s)	1713
NheI	GCTAGC	1 cut(s)	1136
NlaIII	CATG	6 cut(s)	628, 661, 788, 800, 1240, 1662
NlaIV	GGNNCC	7 cut(s)	359, 1071, 1242, 1647, 1700, 1766, 1848
NmeAIII	GCCGAG	1 cut(s)	1204
NmuCI	GTSAC	3 cut(s)	208, 1111, 1117
NsbI	TGCGCA	1 cut(s)	1496
NsiI	ATGCAT	2 cut(s)	786, 1009
NspI	RCATGY	2 cut(s)	788, 1662
PaeR7I	CTCGAG	1 cut(s)	1368
PaqCI	CACCTGC	1 cut(s)	1708
PciI	ACATGT	1 cut(s)	1658
PcsI	WCGNNNNNNNCGW	1 cut(s)	426
PctI	GAATGC	1 cut(s)	786
PdiI	GCCGGC	1 cut(s)	1715
PdmI	GAANNNNTTC	1 cut(s)	187
PfeI	GAWTC	1 cut(s)	1373
PkrI	GCNGC	5 cut(s)	538, 592, 595, 1048, 1888
PleI	GAGTC	4 cut(s)	998, 1517, 1541, 1571
PpsI	GAGTC	4 cut(s)	998, 1517, 1541, 1571
PpuMI	RGGWCCY	1 cut(s)	340
PscI	ACATGT	1 cut(s)	1658
PshAI	GACNNNNGTC	1 cut(s)	446
PshBI	ATTAAT	1 cut(s)	972
Psp124BI	GAGCTC	1 cut(s)	396
Psp5II	RGGWCCY	1 cut(s)	340
Psp6I	CCWGG	5 cut(s)	303, 333, 360, 858, 1554
PspFI	CCCAGC	2 cut(s)	811, 1294
PspGI	CCWGG	5 cut(s)	303, 333, 360, 858, 1554
PspN4I	GGNNCC	7 cut(s)	359, 1071, 1242, 1647, 1700, 1766, 1848
PspPI	GGNCC	2 cut(s)	331, 340
PspPPI	RGGWCCY	1 cut(s)	340
PstI	CTGCAG	2 cut(s)	534, 877
PstNI	CAGNNNCTG	1 cut(s)	1601
PsuI	RGATCY	2 cut(s)	405, 526
PvuII	CAGCTG	1 cut(s)	815
RsaI	GTAC	4 cut(s)	231, 374, 1311, 1358
RsaNI	GTAC	4 cut(s)	230, 373, 1310, 1357
RseI	CAYNNNNRTG	3 cut(s)	177, 623, 1107
SacI	GAGCTC	1 cut(s)	396
SaqAI	TTAA	4 cut(s)	564, 927, 972, 1911
SatI	GCNGC	5 cut(s)	537, 591, 594, 1047, 1887
Sau96I	GGNCC	2 cut(s)	331, 340
SchI	GAGTC	4 cut(s)	999, 1517, 1541, 1571
ScrFI	CCNGG	7 cut(s)	305, 335, 362, 860, 1403, 1556, 1731
SduI	GDGCHC	5 cut(s)	396, 603, 826, 1021, 1563
SfaNI	GCATC	5 cut(s)	542, 640, 975, 994, 1431
SfcI	CTRYAG	2 cut(s)	530, 873
Sfr274I	CTCGAG	1 cut(s)	1368
SinI	GGWCC	1 cut(s)	340
SlaI	CTCGAG	1 cut(s)	1368
SmiMI	CAYNNNNRTG	3 cut(s)	177, 623, 1107
SmlI	CTYRAG	2 cut(s)	1368, 1910
SmoI	CTYRAG	2 cut(s)	1368, 1910
Sse9I	AATT	5 cut(s)	1167, 1313, 1335, 1583, 1872
SsiI	CCGC	6 cut(s)	329, 438, 536, 591, 594, 1029
SspI	AATATT	1 cut(s)	1756
SspMI	CTAG	5 cut(s)	630, 759, 1137, 1392, 1457
SstI	GAGCTC	1 cut(s)	396
StyD4I	CCNGG	7 cut(s)	303, 333, 360, 858, 1401, 1554, 1729
StyI	CCWWGG	2 cut(s)	624, 1236
TaaI	ACNGT	5 cut(s)	229, 234, 586, 672, 1117
TaiI	ACGT	4 cut(s)	64, 766, 941, 1686
TaqI	TCGA	5 cut(s)	265, 465, 473, 855, 1369
TaqII	GACCGA	1 cut(s)	557
TasI	AATT	5 cut(s)	1167, 1313, 1335, 1583, 1872
TatI	WGTACW	2 cut(s)	1309, 1356
TauI	GCSGC	3 cut(s)	539, 593, 596
TfiI	GAWTC	1 cut(s)	1373
Tru1I	TTAA	4 cut(s)	564, 927, 972, 1911
Tru9I	TTAA	4 cut(s)	564, 927, 972, 1911
TscAI	CASTG	1 cut(s)	877
TseFI	GTSAC	3 cut(s)	208, 1111, 1117
TseI	GCWGC	2 cut(s)	1046, 1886
Tsp45I	GTSAC	3 cut(s)	208, 1111, 1117
TspDTI	ATGAA	4 cut(s)	674, 965, 1014, 1438
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	1 cut(s)	877
Vha464I	CTTAAG	1 cut(s)	1910
VneI	GTGCAC	1 cut(s)	1559
VpaK11BI	GGWCC	1 cut(s)	340
VspI	ATTAAT	1 cut(s)	972
XapI	RAATTY	1 cut(s)	1583
XceI	RCATGY	2 cut(s)	788, 1662
XhoI	CTCGAG	1 cut(s)	1368
XmnI	GAANNNNTTC	1 cut(s)	187
XspI	CTAG	5 cut(s)	630, 759, 1137, 1392, 1457
Zsp2I	ATGCAT	2 cut(s)	786, 1009

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.