Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012863

Physical Location & Seq

Genomic Coordinates Forward (+)

12620303 .. 12625139

Gene Structure 4837 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1157900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1896 bp

ATGGCTTCCTCCTCCATCAAGGCCATCTCCTCCTCCGCCTTGACCGCCTCACCGAAGCTGTCTCAAGTCCCTCCACCACCATCCAAACCCTACCTCCTTTCCTTCCTCCACACCACCTCCAACCGCATATCCTTAAAACTCTCCCATTCCCATAATTTGCGTTCCCATTCTTTTCTCATAACCAACGTTCTAAAAACAGCTGAGTCATCATCCGACACATACGTTTCAAAGTCCTCCCAGCTTCCCAAGCAACCGTCTTCAGATTCAACTCCGACCATCCTGGTCTCCGAAAAACTCGGAGAAGCCGGTCTTCATGTCCTAGGTGGCTTTGGCAACTTGGAGTGCGCGTATGACCTCTCCCATGAGGAGCTTTGCGCCAAGATCTCGACATGCGACGCTTTGATCGTTCGGAGCGGAACGAAGGTGACCAGGCAGGTGTTTGAGGCCGCCAAAGGGAGGTTGAAGGTGGTGGGGAGAGCTGGTGTGGGGATAGACAATGTGGATCTGCAGGCGGCCACGGAATATGGATGCCTGGTCGTTAATGCGCCCACGGCAAACACCGTGGCCGCAGCAGAGCACGGGATTGCGCTGCTCACTGCCATGTCACGAAATGTTGCGCAAGCTGATGCTTCCATCAAAGCTGGAAAGTGGCAACGAAACAAGTACATTGGCGTCTCTCTGGTTGGGAAAACATTGGCAGTTATGGGACTTGGAAAAGTTGGATCCGAAGTTGCTAGACGTGCGAAAGGCTTGGGAATGCATGTTATTTCGCATGATCCTTATGCACCGGCTGATAGAGCGCGCGCAATTGGTGTGGATTTGGTCTCTTTTGAGCAGGCAATTGGCACTGCAGATTTCATATCACTGCACATGCCTCTCACCCCTACTACATCAAAGGTGTTCAACGATAAAACATTCGCAATGATGAAGAAGGGAGTCCGAATCATTAATGTTGCCCGAGGCGGAGTCATTGATGAAGATGCACTAGTGAGAGCTCTTGACAGTGGAATTGTTGCTCAGGCCGCACTTGATGTGTTTGAAGAGGAGCCTCCATCCAAAGATAGCAAGCTCGTGCAACATGAGAATGTAACAGTCACACCTCATCTAGGGGCTAGCACAAAGGAAGCTCAGGAAGGTGTTGCCATTGAAATAGCAGAAGCTGTTGTTGGAGCATTGAAAGGTGAACTTTCTGCAACCGCTGTCAACGCTCCCATGGTCTCTCCTGAGGTTCTGGCTGAGTTGTCTCCATATGTTGTGCTAGCCGAGAAGCTGGGAAAGTTGGCTGTACAATTAGTAGCAGGAGGAAGTGGGATTAAATCTGTGAGGGTGGTTTACAAATCAGCTCGCCACCCCGATGACTTGGATACCAGACTTCTCAGGGCCATGATCACTAAAGGCATCATCGAACCGATATCTGCCTCATTTATCAATCTAGTCAATGCAGATTTCATTGCGAAGGATAAAGGTCTGCGCATAAGCGAGGAAAGGGTAGCTGTCGACACATCCCCAGAGTGCCCTGTCGCTTCAATCCAAGTACACATTTCCAATGTGGAGTCCAAATTCGCAAGTTGTGTTTCCGAAAATGGAAACATAAGCATTGAGGGGAAAGTGAAATATGGAAAGCCCCATCTGACATTGTTAGGATCGTTTGGCGTGGACGTGAGCCTCGAGGGAAATCTCATTCTGTGCCGGCAGGTGGATCATCCAGGCATGATTGGGAAGGTGGGGAACATACTTGGGACTGAGAATGTGAATGTGAACTTTATGAGTGTGGGAAGAACTGTCCAGAAGAAGGCCATAATGGCGATTGGAGTGGATGAGGAACCGAAAGAGGAGTTACTCAATAAAATCGGGAAGGTACCTGATATCGAAGAGTTTGTGTTCCTTAAGCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

632

Amino Acids

66.83

Weight (kDa)

7.66

Isoelectric Point (pI)

35.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	93 - 402	6.8e-35	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	195 - 370	1.3e-62	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter	PF19304	414 - 527	4.1e-14	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	561 - 621	6.2e-09	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17745 AT1G17745
fragaria_vesca	FvH4_6g37460
malus_domestica	MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica	Prupe.3G168100_v2.0.a1
pyrus_communis	pycom09g07670 pycom17g13710
rosa_chinensis	RchiOBHm_Chr2g0150301
rosa_laevigata	RLG00000020484
rosa_multiflora	Rmu_sc0002335.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097310
rosa_rugosa	Rorug02G0420400
rosa_samantha	Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana	Rw2G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	424, 1684
AbsI	CCTCGAGG	1 cut(s)	1667
Acc16I	TGCGCA	2 cut(s)	618, 1472
Acc36I	ACCTGC	2 cut(s)	424, 1684
Acc65I	GGTACC	1 cut(s)	1858
AccB1I	GGYRCC	1 cut(s)	1858
AccBSI	CCGCTC	1 cut(s)	414
AccI	GTMKAC	1 cut(s)	1497
AccII	CGCG	3 cut(s)	347, 802, 804
AclI	AACGTT	1 cut(s)	186
AclWI	GGATC	6 cut(s)	510, 717, 730, 770, 1651, 1707
AcoI	YGGCCR	2 cut(s)	513, 564
AcsI	RAATTY	1 cut(s)	1559
AcuI	CTGAAG	1 cut(s)	243
AcyI	GRCGYC	1 cut(s)	672
AfaI	GTAC	4 cut(s)	665, 1287, 1536, 1860
AfiI	CCNNNNNNNGG	5 cut(s)	453, 456, 1106, 1696, 1792
AflII	CTTAAG	1 cut(s)	1886
AgsI	TTSAA	8 cut(s)	228, 267, 463, 904, 1040, 1148, 1177, 1527
AhlI	ACTAGT	1 cut(s)	985
AjiI	CACGTC	2 cut(s)	740, 1660
AjnI	CCWGG	4 cut(s)	279, 428, 531, 1705
AjuI	GAANNNNNNNTTGG	2 cut(s)	1149, 1181
Alw21I	GWGCWC	2 cut(s)	579, 997
Alw26I	GTCTC	6 cut(s)	66, 289, 679, 829, 1222, 1248
AlwI	GGATC	6 cut(s)	510, 717, 730, 770, 1651, 1707
AlwNI	CAGNNNCTG	1 cut(s)	1160
Ama87I	CYCGRG	2 cut(s)	957, 1667
AoxI	GGCC	7 cut(s)	21, 444, 513, 564, 1020, 1380, 1794
ApeKI	GCWGC	2 cut(s)	569, 589
ApoI	RAATTY	1 cut(s)	1559
AseI	ATTAAT	1 cut(s)	948
Asp718I	GGTACC	1 cut(s)	1858
AspA2I	CCTAGG	1 cut(s)	319
AspLEI	GCGC	9 cut(s)	347, 377, 547, 589, 619, 802, 804, 806, 1473
AspS9I	GGNCC	1 cut(s)	1380
AsuHPI	GGTGA	4 cut(s)	42, 436, 871, 1193
AsuNHI	GCTAGC	2 cut(s)	1112, 1258
AvaI	CYCGRG	2 cut(s)	957, 1667
AvrII	CCTAGG	1 cut(s)	319
AxyI	CCTNAGG	1 cut(s)	1224
BaeGI	GKGCMC	1 cut(s)	1517
BamHI	GGATCC	1 cut(s)	722
BanI	GGYRCC	1 cut(s)	1858
BanII	GRGCYC	1 cut(s)	997
BauI	CACGAG	1 cut(s)	1070
BbsI	GAAGAC	2 cut(s)	249, 302
Bbv12I	GWGCWC	2 cut(s)	579, 997
BbvI	GCAGC	2 cut(s)	576, 581
BccI	CCATC	7 cut(s)	23, 32, 88, 284, 641, 1060, 1635
BceAI	ACGGC	1 cut(s)	567
BciT130I	CCWGG	4 cut(s)	281, 430, 533, 1707
BciVI	GTATCC	1 cut(s)	1357
BclI	TGATCA	1 cut(s)	1386
BcoDI	GTCTC	6 cut(s)	66, 289, 679, 829, 1222, 1248
BcuI	ACTAGT	1 cut(s)	985
BfaI	CTAG	7 cut(s)	320, 735, 986, 1106, 1113, 1259, 1433
BfmI	CTRYAG	3 cut(s)	506, 849, 1892
BfrI	CTTAAG	1 cut(s)	1886
BfuAI	ACCTGC	2 cut(s)	424, 1684
BfuI	GTATCC	1 cut(s)	1357
BglI	GCCNNNNNGGC	1 cut(s)	1802
BglII	AGATCT	1 cut(s)	381
BisI	GCNGC	6 cut(s)	447, 513, 567, 570, 590, 1023
BlnI	CCTAGG	1 cut(s)	319
BlsI	GCNGC	6 cut(s)	448, 514, 568, 571, 591, 1024
Bme1390I	CCNGG	4 cut(s)	281, 430, 533, 1707
BmeT110I	CYCGRG	2 cut(s)	957, 1667
BmgBI	CACGTC	2 cut(s)	740, 1660
BmgT120I	GGNCC	1 cut(s)	1380
BmiI	GGNNCC	4 cut(s)	724, 1047, 1824, 1860
BmrFI	CCNGG	4 cut(s)	281, 430, 533, 1707
BmsI	GCATC	4 cut(s)	518, 616, 970, 1407
BmtI	GCTAGC	2 cut(s)	1116, 1262
BpiI	GAAGAC	2 cut(s)	249, 302
Bpu10I	CCTNAGC	2 cut(s)	1017, 1128
BpuEI	CTTGAG	1 cut(s)	48
BsaHI	GRCGYC	1 cut(s)	672
BsaI	GGTCTC	3 cut(s)	289, 829, 1222
BsaJI	CCNNGG	6 cut(s)	319, 516, 549, 561, 958, 1212
BsaXI	ACNNNNNCTCC	8 cut(s)	78, 101, 108, 131, 332, 362, 466, 496
Bsc4I	CCNNNNNNNGG	5 cut(s)	453, 456, 1106, 1696, 1792
Bse118I	RCCGGY	3 cut(s)	305, 787, 1689
Bse21I	CCTNAGG	1 cut(s)	1224
Bse3DI	GCAATG	2 cut(s)	927, 1449
BseBI	CCWGG	4 cut(s)	281, 430, 533, 1707
BseDI	CCNNGG	6 cut(s)	319, 516, 549, 561, 958, 1212
BseGI	GGATG	8 cut(s)	80, 209, 276, 533, 1052, 1502, 1702, 1822
BseLI	CCNNNNNNNGG	5 cut(s)	453, 456, 1106, 1696, 1792
BseMI	GCAATG	2 cut(s)	927, 1449
BseMII	CTCAG	7 cut(s)	192, 1031, 1142, 1215, 1227, 1390, 1734
BsePI	GCGCGC	2 cut(s)	800, 802
BseRI	GAGGAG	5 cut(s)	19, 22, 380, 1058, 1847
BseSI	GKGCMC	1 cut(s)	1517
BseXI	GCAGC	2 cut(s)	576, 581
BseYI	CCCAGC	2 cut(s)	237, 1270
BsgI	GTGCAG	1 cut(s)	851
Bsh1236I	CGCG	3 cut(s)	347, 802, 804
BshFI	GGCC	7 cut(s)	23, 446, 515, 566, 1022, 1382, 1796
BshNI	GGYRCC	1 cut(s)	1858
BsiHKAI	GWGCWC	2 cut(s)	579, 997
BsiHKCI	CYCGRG	2 cut(s)	957, 1667
BsiSI	CCGG	3 cut(s)	306, 788, 1690
BslFI	GGGAC	3 cut(s)	53, 720, 1753
BslI	CCNNNNNNNGG	5 cut(s)	453, 456, 1106, 1696, 1792
BsmAI	GTCTC	6 cut(s)	66, 289, 679, 829, 1222, 1248
BsmBI	CGTCTC	1 cut(s)	679
BsmFI	GGGAC	3 cut(s)	53, 720, 1753
BsmI	GAATGC	1 cut(s)	762
BsnI	GGCC	7 cut(s)	23, 446, 515, 566, 1022, 1382, 1796
Bso31I	GGTCTC	3 cut(s)	289, 829, 1222
BsoBI	CYCGRG	2 cut(s)	957, 1667
Bsp1286I	GDGCHC	3 cut(s)	579, 997, 1517
Bsp1407I	TGTACA	1 cut(s)	1285
Bsp143I	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
Bsp19I	CCATGG	1 cut(s)	1212
BspANI	GGCC	7 cut(s)	23, 446, 515, 566, 1022, 1382, 1796
BspCNI	CTCAG	7 cut(s)	193, 1030, 1141, 1216, 1228, 1389, 1735
BspFNI	CGCG	3 cut(s)	347, 802, 804
BspLI	GGNNCC	4 cut(s)	724, 1047, 1824, 1860
BspMAI	CTGCAG	2 cut(s)	510, 853
BspMI	ACCTGC	2 cut(s)	424, 1684
BspOI	GCTAGC	2 cut(s)	1116, 1262
BspPI	GGATC	6 cut(s)	510, 717, 730, 770, 1651, 1707
BspT107I	GGYRCC	1 cut(s)	1858
BspTI	CTTAAG	1 cut(s)	1886
BspTNI	GGTCTC	3 cut(s)	289, 829, 1222
BsrBI	CCGCTC	1 cut(s)	414
BsrDI	GCAATG	2 cut(s)	927, 1449
BsrFI	RCCGGY	3 cut(s)	305, 787, 1689
BsrGI	TGTACA	1 cut(s)	1285
BssAI	RCCGGY	3 cut(s)	305, 787, 1689
BssECI	CCNNGG	6 cut(s)	319, 516, 549, 561, 958, 1212
BssHII	GCGCGC	2 cut(s)	800, 802
BssMI	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
BssNI	GRCGYC	1 cut(s)	672
BssSI	CACGAG	1 cut(s)	1070
BssT1I	CCWWGG	2 cut(s)	319, 1212
Bst2BI	CACGAG	1 cut(s)	1070
Bst2UI	CCWGG	4 cut(s)	281, 430, 533, 1707
Bst4CI	ACNGT	5 cut(s)	255, 562, 1004, 1093, 1783
Bst6I	CTCTTC	2 cut(s)	1035, 1866
BstACI	GRCGYC	1 cut(s)	672
BstAFI	CTTAAG	1 cut(s)	1886
BstAUI	TGTACA	1 cut(s)	1285
BstDEI	CTNAG	7 cut(s)	201, 1017, 1128, 1224, 1236, 1376, 1743
BstDSI	CCRYGG	4 cut(s)	516, 549, 561, 1212
BstEII	GGTNACC	1 cut(s)	424
BstENI	CCTNNNNNAGG	1 cut(s)	1104
BstF5I	GGATG	8 cut(s)	80, 209, 276, 533, 1052, 1502, 1702, 1822
BstFNI	CGCG	3 cut(s)	347, 802, 804
BstHHI	GCGC	9 cut(s)	347, 377, 547, 589, 619, 802, 804, 806, 1473
BstKTI	GATC	8 cut(s)	384, 405, 505, 725, 778, 1389, 1646, 1702
BstMAI	GTCTC	6 cut(s)	66, 289, 679, 829, 1222, 1248
BstMBI	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 247, 551, 740, 797, 1022, 1205, 1802
BstNI	CCWGG	4 cut(s)	281, 430, 533, 1707
BstNSI	RCATGY	3 cut(s)	393, 764, 874
BstPI	GGTNACC	1 cut(s)	424
BstSCI	CCNGG	4 cut(s)	279, 428, 531, 1705
BstSFI	CTRYAG	3 cut(s)	506, 849, 1892
BstSLI	GKGCMC	1 cut(s)	1517
BstUI	CGCG	3 cut(s)	347, 802, 804
BstV1I	GCAGC	2 cut(s)	576, 581
BstV2I	GAAGAC	2 cut(s)	249, 302
BstX2I	RGATCY	3 cut(s)	381, 502, 722
BstYI	RGATCY	3 cut(s)	381, 502, 722
Bsu36I	CCTNAGG	1 cut(s)	1224
BsuI	GTATCC	1 cut(s)	1357
BsuRI	GGCC	7 cut(s)	23, 446, 515, 566, 1022, 1382, 1796
BtgI	CCRYGG	4 cut(s)	516, 549, 561, 1212
BtrI	CACGTC	2 cut(s)	740, 1660
BtsCI	GGATG	8 cut(s)	80, 209, 276, 533, 1052, 1502, 1702, 1822
BtsI	GCAGTG	3 cut(s)	594, 846, 863
BtsIMutI	CAGTG	4 cut(s)	594, 846, 863, 1009
BveI	ACCTGC	2 cut(s)	424, 1684
CaiI	CAGNNNCTG	1 cut(s)	1160
CfoI	GCGC	9 cut(s)	347, 377, 547, 589, 619, 802, 804, 806, 1473
Cfr10I	RCCGGY	3 cut(s)	305, 787, 1689
Cfr13I	GGNCC	1 cut(s)	1380
CseI	GACGC	2 cut(s)	404, 661
Csp6I	GTAC	4 cut(s)	664, 1286, 1535, 1859
CspCI	CAANNNNNGTGG	4 cut(s)	543, 578, 795, 830
CviQI	GTAC	4 cut(s)	664, 1286, 1535, 1859
DdeI	CTNAG	7 cut(s)	201, 1017, 1128, 1224, 1236, 1376, 1743
DpnI	GATC	8 cut(s)	383, 404, 504, 724, 777, 1388, 1645, 1701
DpnII	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
EaeI	YGGCCR	2 cut(s)	513, 564
Eam1104I	CTCTTC	2 cut(s)	1035, 1866
EarI	CTCTTC	2 cut(s)	1035, 1866
EciI	GGCGGA	2 cut(s)	25, 978
Ecl136II	GAGCTC	1 cut(s)	995
Eco130I	CCWWGG	2 cut(s)	319, 1212
Eco24I	GRGCYC	1 cut(s)	997
Eco31I	GGTCTC	3 cut(s)	289, 829, 1222
Eco32I	GATATC	2 cut(s)	1413, 1867
Eco53kI	GAGCTC	1 cut(s)	995
Eco57I	CTGAAG	1 cut(s)	243
Eco81I	CCTNAGG	1 cut(s)	1224
Eco88I	CYCGRG	2 cut(s)	957, 1667
Eco91I	GGTNACC	1 cut(s)	424
EcoICRI	GAGCTC	1 cut(s)	995
EcoNI	CCTNNNNNAGG	1 cut(s)	1104
EcoO65I	GGTNACC	1 cut(s)	424
EcoRII	CCWGG	4 cut(s)	279, 428, 531, 1705
EcoRV	GATATC	2 cut(s)	1413, 1867
EcoT14I	CCWWGG	2 cut(s)	319, 1212
EcoT22I	ATGCAT	1 cut(s)	762
EcoT38I	GRGCYC	1 cut(s)	997
ErhI	CCWWGG	2 cut(s)	319, 1212
Esp3I	CGTCTC	1 cut(s)	679
FalI	AAGNNNNNCTT	4 cut(s)	294, 326, 1170, 1202
FaqI	GGGAC	3 cut(s)	53, 720, 1753
FauNDI	CATATG	1 cut(s)	1249
FbaI	TGATCA	1 cut(s)	1386
FblI	GTMKAC	1 cut(s)	1497
Fnu4HI	GCNGC	6 cut(s)	447, 513, 567, 570, 590, 1023
FokI	GGATG	8 cut(s)	67, 196, 263, 540, 1039, 1489, 1689, 1829
FriOI	GRGCYC	1 cut(s)	997
Fsp4HI	GCNGC	6 cut(s)	447, 513, 567, 570, 590, 1023
FspBI	CTAG	7 cut(s)	320, 735, 986, 1106, 1113, 1259, 1433
FspI	TGCGCA	2 cut(s)	618, 1472
GlaI	GCGC	9 cut(s)	346, 376, 546, 588, 618, 801, 803, 805, 1472
GluI	GCNGC	6 cut(s)	447, 513, 567, 570, 590, 1023
GsaI	CCCAGC	2 cut(s)	241, 1274
HaeIII	GGCC	7 cut(s)	23, 446, 515, 566, 1022, 1382, 1796
HapII	CCGG	3 cut(s)	306, 788, 1690
HgaI	GACGC	2 cut(s)	404, 661
HhaI	GCGC	9 cut(s)	347, 377, 547, 589, 619, 802, 804, 806, 1473
Hin1I	GRCGYC	1 cut(s)	672
Hin6I	GCGC	9 cut(s)	345, 375, 545, 587, 617, 800, 802, 804, 1471
HinP1I	GCGC	9 cut(s)	345, 375, 545, 587, 617, 800, 802, 804, 1471
HincII	GTYRAC	2 cut(s)	1204, 1498
HindII	GTYRAC	2 cut(s)	1204, 1498
HinfI	GANTC	6 cut(s)	203, 263, 936, 942, 966, 1553
HpaII	CCGG	3 cut(s)	306, 788, 1690
HphI	GGTGA	4 cut(s)	42, 436, 871, 1193
Hpy166II	GTNNAC	7 cut(s)	1184, 1204, 1333, 1498, 1537, 1657, 1759
Hpy188III	TCNNGA	7 cut(s)	385, 606, 998, 1130, 1223, 1786, 1852
Hpy8I	GTNNAC	7 cut(s)	1184, 1204, 1333, 1498, 1537, 1657, 1759
Hpy99I	CGWCG	1 cut(s)	398
HpyAV	CCTTC	9 cut(s)	112, 415, 457, 925, 1127, 1450, 1714, 1786, 1849
HpyCH4III	ACNGT	5 cut(s)	255, 562, 1004, 1093, 1783
HpyCH4IV	ACGT	4 cut(s)	186, 222, 739, 1659
HpyCH4V	TGCA	9 cut(s)	508, 760, 785, 851, 868, 983, 1075, 1193, 1442
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 247, 551, 740, 797, 1022, 1205, 1802
HpyF3I	CTNAG	7 cut(s)	201, 1017, 1128, 1224, 1236, 1376, 1743
HpySE526I	ACGT	4 cut(s)	186, 222, 739, 1659
Hsp92I	GRCGYC	1 cut(s)	672
HspAI	GCGC	9 cut(s)	345, 375, 545, 587, 617, 800, 802, 804, 1471
KpnI	GGTACC	1 cut(s)	1862
KroI	GCCGGC	1 cut(s)	1689
KroNI	GCCGGC	1 cut(s)	1691
Ksp22I	TGATCA	1 cut(s)	1386
Kzo9I	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
LmnI	GCTCC	5 cut(s)	367, 411, 1045, 1169, 1213
Lsp1109I	GCAGC	2 cut(s)	576, 581
LweI	GCATC	4 cut(s)	518, 616, 970, 1407
MaeI	CTAG	7 cut(s)	320, 735, 986, 1106, 1113, 1259, 1433
MaeII	ACGT	4 cut(s)	186, 222, 739, 1659
MaeIII	GTNAC	5 cut(s)	424, 603, 1087, 1093, 1836
MalI	GATC	8 cut(s)	383, 404, 504, 724, 777, 1388, 1645, 1701
MbiI	CCGCTC	1 cut(s)	414
MboI	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
MboII	GAAGA	8 cut(s)	249, 302, 940, 989, 1052, 1788, 1801, 1883
MfeI	CAATTG	2 cut(s)	807, 840
MflI	RGATCY	3 cut(s)	381, 502, 722
MhlI	GDGCHC	3 cut(s)	579, 997, 1517
MluCI	AATT	6 cut(s)	154, 807, 840, 1008, 1289, 1559
MlyI	GAGTC	4 cut(s)	212, 945, 975, 1562
MmeI	TCCRAC	5 cut(s)	144, 237, 296, 700, 1147
Mph1103I	ATGCAT	1 cut(s)	762
MroNI	GCCGGC	1 cut(s)	1689
MseI	TTAA	5 cut(s)	134, 540, 948, 1314, 1887
MslI	CAYNNNNRTG	3 cut(s)	599, 1083, 1353
MspA1I	CMGCKG	2 cut(s)	200, 1199
MspCI	CTTAAG	1 cut(s)	1886
MspI	CCGG	3 cut(s)	306, 788, 1690
MspR9I	CCNGG	4 cut(s)	281, 430, 533, 1707
MunI	CAATTG	2 cut(s)	807, 840
Mva1269I	GAATGC	1 cut(s)	762
MvaI	CCWGG	4 cut(s)	281, 430, 533, 1707
MvnI	CGCG	3 cut(s)	347, 802, 804
MwoI	GCNNNNNNNGC	8 cut(s)	44, 247, 551, 740, 797, 1022, 1205, 1802
NaeI	GCCGGC	1 cut(s)	1691
NcoI	CCATGG	1 cut(s)	1212
NdeI	CATATG	1 cut(s)	1249
NdeII	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
NgoMIV	GCCGGC	1 cut(s)	1689
NheI	GCTAGC	2 cut(s)	1112, 1258
NlaIV	GGNNCC	4 cut(s)	724, 1047, 1824, 1860
NmeAIII	GCCGAG	1 cut(s)	1288
NmuCI	GTSAC	3 cut(s)	424, 603, 1093
NsbI	TGCGCA	2 cut(s)	618, 1472
NsiI	ATGCAT	1 cut(s)	762
NspI	RCATGY	3 cut(s)	393, 764, 874
PaeR7I	CTCGAG	1 cut(s)	1667
PaqCI	CACCTGC	2 cut(s)	424, 1684
PauI	GCGCGC	2 cut(s)	800, 802
PcsI	WCGNNNNNNNCGW	2 cut(s)	402, 1665
PctI	GAATGC	1 cut(s)	762
PdiI	GCCGGC	1 cut(s)	1691
PfeI	GAWTC	2 cut(s)	263, 942
PkrI	GCNGC	6 cut(s)	448, 514, 568, 571, 591, 1024
PleI	GAGTC	4 cut(s)	211, 944, 974, 1561
PpsI	GAGTC	4 cut(s)	211, 944, 974, 1561
PshBI	ATTAAT	1 cut(s)	948
Psp124BI	GAGCTC	1 cut(s)	997
Psp1406I	AACGTT	1 cut(s)	186
Psp6I	CCWGG	4 cut(s)	279, 428, 531, 1705
PspEI	GGTNACC	1 cut(s)	424
PspFI	CCCAGC	2 cut(s)	237, 1270
PspGI	CCWGG	4 cut(s)	279, 428, 531, 1705
PspN4I	GGNNCC	4 cut(s)	724, 1047, 1824, 1860
PspPI	GGNCC	1 cut(s)	1380
PspXI	VCTCGAGB	1 cut(s)	1667
PstI	CTGCAG	2 cut(s)	510, 853
PstNI	CAGNNNCTG	1 cut(s)	1160
PsuI	RGATCY	3 cut(s)	381, 502, 722
PteI	GCGCGC	2 cut(s)	800, 802
PvuII	CAGCTG	1 cut(s)	200
RsaI	GTAC	4 cut(s)	665, 1287, 1536, 1860
RsaNI	GTAC	4 cut(s)	664, 1286, 1535, 1859
RseI	CAYNNNNRTG	3 cut(s)	599, 1083, 1353
SacI	GAGCTC	1 cut(s)	997
SalI	GTCGAC	1 cut(s)	1496
SaqAI	TTAA	5 cut(s)	134, 540, 948, 1314, 1887
SatI	GCNGC	6 cut(s)	447, 513, 567, 570, 590, 1023
Sau3AI	GATC	8 cut(s)	381, 402, 502, 722, 775, 1386, 1643, 1699
Sau96I	GGNCC	1 cut(s)	1380
SchI	GAGTC	4 cut(s)	212, 945, 975, 1562
ScrFI	CCNGG	4 cut(s)	281, 430, 533, 1707
SduI	GDGCHC	3 cut(s)	579, 997, 1517
SfaNI	GCATC	4 cut(s)	518, 616, 970, 1407
SfcI	CTRYAG	3 cut(s)	506, 849, 1892
Sfr274I	CTCGAG	1 cut(s)	1667
SlaI	CTCGAG	1 cut(s)	1667
SmiMI	CAYNNNNRTG	3 cut(s)	599, 1083, 1353
SmlI	CTYRAG	3 cut(s)	63, 1667, 1886
SmoI	CTYRAG	3 cut(s)	63, 1667, 1886
SpeI	ACTAGT	1 cut(s)	985
Sse9I	AATT	6 cut(s)	154, 807, 840, 1008, 1289, 1559
SspMI	CTAG	7 cut(s)	320, 735, 986, 1106, 1113, 1259, 1433
SstI	GAGCTC	1 cut(s)	997
StyD4I	CCNGG	4 cut(s)	279, 428, 531, 1705
StyI	CCWWGG	2 cut(s)	319, 1212
TaaI	ACNGT	5 cut(s)	255, 562, 1004, 1093, 1783
TaiI	ACGT	4 cut(s)	189, 225, 742, 1662
TaqI	TCGA	5 cut(s)	386, 1404, 1497, 1668, 1869
TasI	AATT	6 cut(s)	154, 807, 840, 1008, 1289, 1559
TatI	WGTACW	3 cut(s)	663, 1285, 1534
TauI	GCSGC	4 cut(s)	449, 515, 569, 1025
TfiI	GAWTC	2 cut(s)	263, 942
Tru1I	TTAA	5 cut(s)	134, 540, 948, 1314, 1887
Tru9I	TTAA	5 cut(s)	134, 540, 948, 1314, 1887
TscAI	CASTG	4 cut(s)	601, 853, 870, 1009
TseFI	GTSAC	3 cut(s)	424, 603, 1093
TseI	GCWGC	2 cut(s)	569, 589
Tsp45I	GTSAC	3 cut(s)	424, 603, 1093
TspDTI	ATGAA	5 cut(s)	302, 847, 941, 990, 1438
TspGWI	ACGGA	1 cut(s)	533
TspRI	CASTG	4 cut(s)	601, 853, 870, 1009
Vha464I	CTTAAG	1 cut(s)	1886
VspI	ATTAAT	1 cut(s)	948
XagI	CCTNNNNNAGG	1 cut(s)	1104
XapI	RAATTY	1 cut(s)	1559
XceI	RCATGY	3 cut(s)	393, 764, 874
XhoI	CTCGAG	1 cut(s)	1667
XmaJI	CCTAGG	1 cut(s)	319
XmiI	GTMKAC	1 cut(s)	1497
XspI	CTAG	7 cut(s)	320, 735, 986, 1106, 1113, 1259, 1433
Zsp2I	ATGCAT	1 cut(s)	762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.