Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0012863

Physical Location & Seq

Genomic Coordinates Reverse (-)

69889783 .. 69894349

Gene Structure 4567 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG479300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGCTTCTTCTTCTTCTCTCAAAGCCATCTCCACCTTCACCACCCCTTCAAAAACACCACCACCTCCCTCATCATCATCATCAAAAGCTTCACTTCTCTCCTTCCTCAACAACACCTCCTCTCCAATCTCCCTAAAACTTTCTCACTCCATTCCTCGTTCACATATTCGTGGAAACCATTCCACTCTATCTCTCGCAGTCACCAATGCTTTGAACACCGTACAGTCTTCCTCTCCGGTGTCCACCTCCGGAGATCGAACAAAGTTGCCATCCTCCAACGCCAAGCCTACGATCCTGGTCTCCGAGAAACTTGGAGAGGCGGGCCTGGAGGTCCTACGCAAGTTCGGGAACCTGGAGTGCGCGTACAACCTCACGCCGGAGGAGCTCTGCGCCAAGATCTCAGCCTGCGACGCCCTGATCGTCAGGAGCGGGACGAAGGTCAACAGGCAGGTTTTCGAGGCGTCGAAGGGGAGGCTGAAGGTGGTGGGAAGGGCAGGGGTTGGAATAGACAATGTGGATCTGCAGGCGGCGACGGAGTTTGGATGCCTCGTCGTTAATGCGCCGACAGCAAACACCGTCGCTGCCGCAGAGCACGGGATCGCTCTGCTCACTTCCATGGCTAGAAATGTTGCTCAGGCAGATGCCTCCATGAAAGCTGGACTATGGAAACGCAACAAGTATGTCGGTGTCTCTCTTGTTGGGAAGACATTAGCAGTAATGGGATTTGGAAAAGTTGGATCAGAAGTTGCTAGACGTGCAAAAGGCTTGGGAATGCATGTTATAGCACATGATCCATATGCCCCAGCTGACAGAGCCCGTGCCGTTGGAGTGGATTTGGTCTCTTTCGACCAGGCCATCTCCACTGCAGATTTCGTGTCTCTGCATATGCCACTTACCCCTACTACATCAAAGGTGTTTAATGATGATACGTTTTCAAAGATGAAGAAGGGTGTTCGCATCATTAATGTTGCAAGGGGTGGAGTCATTGATGAAGATGCATTAGTGAGAGCCCTTGATAGCGGAGTTGTAGCTCAGGCTGCACTTGATGTGTTCACAGAGGAGCCTCCATCAAAAGAAAGCAAGTTAGTGCAACATGAGAATGTCACAGTCACACCTCATCTCGGAGCTAGTACAAAGGAAGCACAAGAAGGTGTAGCAATTGAAATAGCCGAGGCAGTAGTTGGAGCATTGCAAGGTGAACTTTCTGCAACTGCTGTCAATGCACCCATGGTTCCTCCTGAGGTTATGTTCGAGTTGTCTCCTTATGTTGTTCTTGCTGAGAAGCTGGGAAAGTTAGCTGTACAATTAGTAGCAGGAGGGAGTGGAATTACATCTGTGAGGGTGGTGTACAAATCAGCTCGAGATTCTGATGACTTGGATACTAGACTTCTCCGGGCTATGATCACTAAAGGCATCATTGAACCGATATCTACCTCATTCATCAATCTAGTAAATGCTGATTATGTTGCCAAGCAGAAAGGTCTGCGCATAAGTGAGGAAAAGGTATCTGTTGACTCATCTCCTGAGTTCCCTGTTGACTCAATCCAGGTGCACATATCCAATGTGGACTCCAAATTTGCAAGTTCAGTTTCTGATAATGGAAGCATAAGCATTGAGGGGAAAGTGAAATATGGGGAACCCCATCTCACATGTTTGGGTTCGTTTGGCGTGGACGTGAGCCTTGAAGGGAATCTTATTTTGTGCCGGCAGGTGGATCAACCCGGAATGATTGGCAAGGTGGGAAATATTCTTGGGAGCCAGAATGTGAATGTGAGCTTTATGAGTGTGGGAAGAACCATCCTGAAGAAAGCCATAATGGCTATTGGTGTAGATGAGGAACCAAACAAGGAGACTTTGAAGAAAATTGGGGATGTGGCTGCTATTGAAGAGTTTGTGTTCCTTAAGCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

636

Amino Acids

66.98

Weight (kDa)

8.06

Isoelectric Point (pI)

38.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	98 - 407	2.2e-34	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	200 - 375	5.5e-63	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
PGDH_inter	PF19304	419 - 522	8.5e-14	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	566 - 625	2.2e-09	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17745 AT1G17745
fragaria_vesca	FvH4_6g37460
malus_domestica	MD09G1157900.v1.1 MD17G1143900.v1.1
prunus_persica	Prupe.3G168100_v2.0.a1
pyrus_communis	pycom09g07670 pycom17g13710
rosa_chinensis	RchiOBHm_Chr2g0150301
rosa_laevigata	RLG00000020484
rosa_multiflora	Rmu_sc0002335.1_g000001
rosa_roxburghii	Rroxscaffold_2G00097310
rosa_rugosa	Rorug02G0420400
rosa_samantha	Rh2AG479300 Rh2BG491600 Rh2CG465500 Rh2DG500400
rosa_wichuraiana	Rw2G039120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1699
Acc16I	TGCGCA	1 cut(s)	1487
Acc36I	ACCTGC	2 cut(s)	439, 1699
AccBSI	CCGCTC	1 cut(s)	429
AccII	CGCG	1 cut(s)	362
AccIII	TCCGGA	1 cut(s)	248
AciI	CCGC	5 cut(s)	320, 429, 527, 585, 1020
AclWI	GGATC	6 cut(s)	286, 525, 605, 745, 785, 1722
AcsI	RAATTY	1 cut(s)	1574
AcuI	CTGAAG	2 cut(s)	497, 1823
AcyI	GRCGYC	2 cut(s)	411, 461
AfaI	GTAC	5 cut(s)	222, 365, 1132, 1302, 1349
AfiI	CCNNNNNNNGG	3 cut(s)	376, 1121, 1711
AflII	CTTAAG	1 cut(s)	1901
AflIII	ACRYGT	1 cut(s)	1649
AgsI	TTSAA	8 cut(s)	51, 214, 936, 1163, 1421, 1685, 1858, 1886
AjiI	CACGTC	2 cut(s)	755, 1675
AjnI	CCWGG	5 cut(s)	294, 324, 351, 849, 1545
Alw21I	GWGCWC	3 cut(s)	387, 594, 1554
Alw26I	GTCTC	6 cut(s)	304, 694, 844, 882, 1263, 1844
Alw44I	GTGCAC	1 cut(s)	1550
AlwI	GGATC	6 cut(s)	286, 525, 605, 745, 785, 1722
AlwNI	CAGNNNCTG	1 cut(s)	1592
Ama87I	CYCGRG	1 cut(s)	1359
Aor13HI	TCCGGA	1 cut(s)	248
AoxI	GGCC	2 cut(s)	322, 852
ApaLI	GTGCAC	1 cut(s)	1550
ApeKI	GCWGC	3 cut(s)	581, 1037, 1877
ApoI	RAATTY	1 cut(s)	1574
ArsI	GACNNNNNNTTYG	2 cut(s)	1559, 1591
AseI	ATTAAT	1 cut(s)	963
Asp700I	GAANNNNTTC	1 cut(s)	178
AspLEI	GCGC	4 cut(s)	362, 392, 562, 1488
AspS9I	GGNCC	2 cut(s)	322, 331
AsuC2I	CCSGG	2 cut(s)	1394, 1722
AsuHPI	GGTGA	3 cut(s)	31, 193, 1208
AvaI	CYCGRG	1 cut(s)	1359
AvaII	GGWCC	1 cut(s)	331
AxyI	CCTNAGG	1 cut(s)	1239
BaeGI	GKGCMC	1 cut(s)	1554
BanII	GRGCYC	3 cut(s)	387, 817, 1012
BbsI	GAAGAC	2 cut(s)	219, 710
Bbv12I	GWGCWC	3 cut(s)	387, 594, 1554
BbvI	GCAGC	3 cut(s)	568, 1024, 1864
BccI	CCATC	6 cut(s)	35, 277, 863, 1075, 1650, 1805
BceAI	ACGGC	1 cut(s)	806
BciT130I	CCWGG	5 cut(s)	296, 326, 353, 851, 1547
BciVI	GTATCC	1 cut(s)	1372
BclI	TGATCA	1 cut(s)	1401
BcnI	CCSGG	2 cut(s)	1394, 1722
BcoDI	GTCTC	6 cut(s)	304, 694, 844, 882, 1263, 1844
BfaI	CTAG	5 cut(s)	621, 750, 1128, 1383, 1448
BfmI	CTRYAG	3 cut(s)	521, 864, 1907
BfrI	CTTAAG	1 cut(s)	1901
BfuAI	ACCTGC	2 cut(s)	439, 1699
BfuI	GTATCC	1 cut(s)	1372
BglI	GCCNNNNNGGC	1 cut(s)	1817
BglII	AGATCT	1 cut(s)	396
BisI	GCNGC	5 cut(s)	528, 582, 585, 1038, 1878
BlsI	GCNGC	5 cut(s)	529, 583, 586, 1039, 1879
Bme1390I	CCNGG	7 cut(s)	296, 326, 353, 851, 1394, 1547, 1722
Bme18I	GGWCC	1 cut(s)	331
BmeT110I	CYCGRG	1 cut(s)	1359
BmgBI	CACGTC	2 cut(s)	755, 1675
BmgT120I	GGNCC	2 cut(s)	322, 331
BmiI	GGNNCC	6 cut(s)	350, 1062, 1233, 1638, 1757, 1839
BmrFI	CCNGG	7 cut(s)	296, 326, 353, 851, 1394, 1547, 1722
BmsI	GCATC	5 cut(s)	533, 631, 966, 985, 1422
BoxI	GACNNNNGTC	1 cut(s)	437
BpiI	GAAGAC	2 cut(s)	219, 710
BpmI	CTGGAG	2 cut(s)	347, 374
Bpu10I	CCTNAGC	2 cut(s)	633, 1032
BpuMI	CCSGG	2 cut(s)	1394, 1722
BsaHI	GRCGYC	2 cut(s)	411, 461
BsaI	GGTCTC	2 cut(s)	304, 844
BsaJI	CCNNGG	3 cut(s)	615, 1170, 1227
BsaWI	WCCGGW	2 cut(s)	235, 248
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 106, 131, 136, 347, 377
Bsc4I	CCNNNNNNNGG	3 cut(s)	376, 1121, 1711
Bse118I	RCCGGY	1 cut(s)	1704
Bse21I	CCTNAGG	1 cut(s)	1239
Bse3DI	GCAATG	1 cut(s)	1187
BseAI	TCCGGA	1 cut(s)	248
BseBI	CCWGG	5 cut(s)	296, 326, 353, 851, 1547
BseDI	CCNNGG	3 cut(s)	615, 1170, 1227
BseGI	GGATG	4 cut(s)	269, 548, 1797, 1876
BseLI	CCNNNNNNNGG	3 cut(s)	376, 1121, 1711
BseMI	GCAATG	1 cut(s)	1187
BseMII	CTCAG	6 cut(s)	414, 647, 1046, 1230, 1269, 1515
BseRI	GAGGAG	3 cut(s)	109, 395, 1073
BseSI	GKGCMC	1 cut(s)	1554
BseXI	GCAGC	3 cut(s)	568, 1024, 1864
BseYI	CCCAGC	2 cut(s)	802, 1285
BsgI	GTGCAG	1 cut(s)	1023
Bsh1236I	CGCG	1 cut(s)	362
BshFI	GGCC	2 cut(s)	324, 854
BsiHKAI	GWGCWC	3 cut(s)	387, 594, 1554
BsiHKCI	CYCGRG	1 cut(s)	1359
BsiSI	CCGG	6 cut(s)	236, 249, 377, 1393, 1705, 1722
BslFI	GGGAC	1 cut(s)	445
BslI	CCNNNNNNNGG	3 cut(s)	376, 1121, 1711
BsmAI	GTCTC	6 cut(s)	304, 694, 844, 882, 1263, 1844
BsmFI	GGGAC	1 cut(s)	445
BsmI	GAATGC	1 cut(s)	777
BsnI	GGCC	2 cut(s)	324, 854
Bso31I	GGTCTC	2 cut(s)	304, 844
BsoBI	CYCGRG	1 cut(s)	1359
Bsp1286I	GDGCHC	5 cut(s)	387, 594, 817, 1012, 1554
Bsp13I	TCCGGA	1 cut(s)	248
Bsp1407I	TGTACA	2 cut(s)	1300, 1347
Bsp19I	CCATGG	2 cut(s)	615, 1227
BspACI	CCGC	5 cut(s)	320, 429, 527, 585, 1020
BspANI	GGCC	2 cut(s)	324, 854
BspCNI	CTCAG	6 cut(s)	413, 646, 1045, 1231, 1270, 1516
BspEI	TCCGGA	1 cut(s)	248
BspFNI	CGCG	1 cut(s)	362
BspLI	GGNNCC	6 cut(s)	350, 1062, 1233, 1638, 1757, 1839
BspMAI	CTGCAG	2 cut(s)	525, 868
BspMI	ACCTGC	2 cut(s)	439, 1699
BspPI	GGATC	6 cut(s)	286, 525, 605, 745, 785, 1722
BspTI	CTTAAG	1 cut(s)	1901
BspTNI	GGTCTC	2 cut(s)	304, 844
BsrBI	CCGCTC	1 cut(s)	429
BsrDI	GCAATG	1 cut(s)	1187
BsrFI	RCCGGY	1 cut(s)	1704
BsrGI	TGTACA	2 cut(s)	1300, 1347
BssAI	RCCGGY	1 cut(s)	1704
BssECI	CCNNGG	3 cut(s)	615, 1170, 1227
BssNI	GRCGYC	2 cut(s)	411, 461
BssT1I	CCWWGG	2 cut(s)	615, 1227
Bst2UI	CCWGG	5 cut(s)	296, 326, 353, 851, 1547
Bst4CI	ACNGT	4 cut(s)	220, 225, 577, 1108
Bst6I	CTCTTC	1 cut(s)	1881
BstACI	GRCGYC	2 cut(s)	411, 461
BstAFI	CTTAAG	1 cut(s)	1901
BstAUI	TGTACA	2 cut(s)	1300, 1347
BstC8I	GCNNGC	4 cut(s)	322, 406, 525, 1706
BstDEI	CTNAG	6 cut(s)	400, 633, 1032, 1239, 1278, 1524
BstDSI	CCRYGG	2 cut(s)	615, 1227
BstF5I	GGATG	4 cut(s)	269, 548, 1797, 1876
BstFNI	CGCG	1 cut(s)	362
BstHHI	GCGC	4 cut(s)	362, 392, 562, 1488
BstMAI	GTCTC	6 cut(s)	304, 694, 844, 882, 1263, 1844
BstMWI	GCNNNNNNNGC	8 cut(s)	382, 410, 566, 755, 812, 1037, 1220, 1817
BstNI	CCWGG	5 cut(s)	296, 326, 353, 851, 1547
BstNSI	RCATGY	2 cut(s)	779, 1653
BstPAI	GACNNNNGTC	1 cut(s)	437
BstSCI	CCNGG	7 cut(s)	294, 324, 351, 849, 1392, 1545, 1720
BstSFI	CTRYAG	3 cut(s)	521, 864, 1907
BstSLI	GKGCMC	1 cut(s)	1554
BstUI	CGCG	1 cut(s)	362
BstV1I	GCAGC	3 cut(s)	568, 1024, 1864
BstV2I	GAAGAC	2 cut(s)	219, 710
BstX2I	RGATCY	2 cut(s)	396, 517
BstYI	RGATCY	2 cut(s)	396, 517
Bsu36I	CCTNAGG	1 cut(s)	1239
BsuI	GTATCC	1 cut(s)	1372
BsuRI	GGCC	2 cut(s)	324, 854
BtgI	CCRYGG	2 cut(s)	615, 1227
BtrI	CACGTC	2 cut(s)	755, 1675
BtsCI	GGATG	4 cut(s)	269, 548, 1797, 1876
BtsI	GCAGTG	1 cut(s)	861
BtsIMutI	CAGTG	1 cut(s)	861
BveI	ACCTGC	2 cut(s)	439, 1699
Cac8I	GCNNGC	4 cut(s)	322, 406, 525, 1706
CaiI	CAGNNNCTG	1 cut(s)	1592
CfoI	GCGC	4 cut(s)	362, 392, 562, 1488
Cfr10I	RCCGGY	1 cut(s)	1704
Cfr13I	GGNCC	2 cut(s)	322, 331
CseI	GACGC	2 cut(s)	419, 450
Csp6I	GTAC	5 cut(s)	221, 364, 1131, 1301, 1348
CviAII	CATG	7 cut(s)	616, 649, 776, 788, 1094, 1228, 1650
CviQI	GTAC	5 cut(s)	221, 364, 1131, 1301, 1348
DdeI	CTNAG	6 cut(s)	400, 633, 1032, 1239, 1278, 1524
Eam1104I	CTCTTC	1 cut(s)	1881
EarI	CTCTTC	1 cut(s)	1881
Ecl136II	GAGCTC	1 cut(s)	385
Eco130I	CCWWGG	2 cut(s)	615, 1227
Eco24I	GRGCYC	3 cut(s)	387, 817, 1012
Eco31I	GGTCTC	2 cut(s)	304, 844
Eco32I	GATATC	1 cut(s)	1428
Eco47I	GGWCC	1 cut(s)	331
Eco53kI	GAGCTC	1 cut(s)	385
Eco57I	CTGAAG	2 cut(s)	497, 1823
Eco81I	CCTNAGG	1 cut(s)	1239
Eco88I	CYCGRG	1 cut(s)	1359
EcoICRI	GAGCTC	1 cut(s)	385
EcoO109I	RGGNCCY	1 cut(s)	331
EcoRII	CCWGG	5 cut(s)	294, 324, 351, 849, 1545
EcoRV	GATATC	1 cut(s)	1428
EcoT14I	CCWWGG	2 cut(s)	615, 1227
EcoT22I	ATGCAT	2 cut(s)	777, 1000
EcoT38I	GRGCYC	3 cut(s)	387, 817, 1012
ErhI	CCWWGG	2 cut(s)	615, 1227
FaeI	CATG	7 cut(s)	619, 652, 779, 791, 1097, 1231, 1653
FalI	AAGNNNNNCTT	6 cut(s)	78, 110, 1185, 1217, 1677, 1709
FaqI	GGGAC	1 cut(s)	445
FatI	CATG	7 cut(s)	615, 648, 775, 787, 1093, 1227, 1649
FauI	CCCGC	2 cut(s)	313, 422
FauNDI	CATATG	2 cut(s)	796, 885
FbaI	TGATCA	1 cut(s)	1401
Fnu4HI	GCNGC	5 cut(s)	528, 582, 585, 1038, 1878
FokI	GGATG	4 cut(s)	256, 555, 1784, 1883
FriOI	GRGCYC	3 cut(s)	387, 817, 1012
Fsp4HI	GCNGC	5 cut(s)	528, 582, 585, 1038, 1878
FspBI	CTAG	5 cut(s)	621, 750, 1128, 1383, 1448
FspI	TGCGCA	1 cut(s)	1487
GlaI	GCGC	4 cut(s)	361, 391, 561, 1487
GluI	GCNGC	5 cut(s)	528, 582, 585, 1038, 1878
GsaI	CCCAGC	2 cut(s)	806, 1289
GsuI	CTGGAG	2 cut(s)	347, 374
HaeIII	GGCC	2 cut(s)	324, 854
HapII	CCGG	6 cut(s)	236, 249, 377, 1393, 1705, 1722
HgaI	GACGC	2 cut(s)	419, 450
HhaI	GCGC	4 cut(s)	362, 392, 562, 1488
Hin1I	GRCGYC	2 cut(s)	411, 461
Hin1II	CATG	7 cut(s)	619, 652, 779, 791, 1097, 1231, 1653
Hin6I	GCGC	4 cut(s)	360, 390, 560, 1486
HinP1I	GCGC	4 cut(s)	360, 390, 560, 1486
HincII	GTYRAC	3 cut(s)	442, 1513, 1537
HindII	GTYRAC	3 cut(s)	442, 1513, 1537
HindIII	AAGCTT	1 cut(s)	87
HinfI	GANTC	6 cut(s)	981, 1364, 1514, 1538, 1568, 1690
HpaII	CCGG	6 cut(s)	236, 249, 377, 1393, 1705, 1722
HphI	GGTGA	3 cut(s)	31, 193, 1208
Hpy188I	TCNGA	5 cut(s)	304, 742, 1124, 1369, 1594
Hpy188III	TCNNGA	7 cut(s)	249, 346, 424, 1238, 1361, 1523, 1801
Hpy99I	CGWCG	5 cut(s)	413, 466, 535, 554, 581
HpyCH4III	ACNGT	4 cut(s)	220, 225, 577, 1108
HpyCH4IV	ACGT	3 cut(s)	754, 929, 1674
HpyF10VI	GCNNNNNNNGC	8 cut(s)	382, 410, 566, 755, 812, 1037, 1220, 1817
HpyF3I	CTNAG	6 cut(s)	400, 633, 1032, 1239, 1278, 1524
HpySE526I	ACGT	3 cut(s)	754, 929, 1674
Hsp92I	GRCGYC	2 cut(s)	411, 461
Hsp92II	CATG	7 cut(s)	619, 652, 779, 791, 1097, 1231, 1653
HspAI	GCGC	4 cut(s)	360, 390, 560, 1486
Kpn2I	TCCGGA	1 cut(s)	248
KroI	GCCGGC	1 cut(s)	1704
KroNI	GCCGGC	1 cut(s)	1706
Ksp22I	TGATCA	1 cut(s)	1401
LmnI	GCTCC	6 cut(s)	382, 426, 1060, 1124, 1184, 1755
Lsp1109I	GCAGC	3 cut(s)	568, 1024, 1864
LweI	GCATC	5 cut(s)	533, 631, 966, 985, 1422
MaeI	CTAG	5 cut(s)	621, 750, 1128, 1383, 1448
MaeII	ACGT	3 cut(s)	754, 929, 1674
MaeIII	GTNAC	3 cut(s)	199, 1102, 1108
MbiI	CCGCTC	1 cut(s)	429
MfeI	CAATTG	1 cut(s)	1158
MflI	RGATCY	2 cut(s)	396, 517
MhlI	GDGCHC	5 cut(s)	387, 594, 817, 1012, 1554
MluCI	AATT	5 cut(s)	1158, 1304, 1326, 1574, 1863
MlyI	GAGTC	4 cut(s)	990, 1508, 1532, 1562
MmeI	TCCRAC	5 cut(s)	300, 481, 715, 805, 1162
Mph1103I	ATGCAT	2 cut(s)	777, 1000
MroI	TCCGGA	1 cut(s)	248
MroNI	GCCGGC	1 cut(s)	1704
MroXI	GAANNNNTTC	1 cut(s)	178
MseI	TTAA	4 cut(s)	555, 918, 963, 1902
MslI	CAYNNNNRTG	3 cut(s)	168, 614, 1098
MspA1I	CMGCKG	1 cut(s)	806
MspCI	CTTAAG	1 cut(s)	1901
MspI	CCGG	6 cut(s)	236, 249, 377, 1393, 1705, 1722
MspR9I	CCNGG	7 cut(s)	296, 326, 353, 851, 1394, 1547, 1722
MunI	CAATTG	1 cut(s)	1158
Mva1269I	GAATGC	1 cut(s)	777
MvaI	CCWGG	5 cut(s)	296, 326, 353, 851, 1547
MvnI	CGCG	1 cut(s)	362
MwoI	GCNNNNNNNGC	8 cut(s)	382, 410, 566, 755, 812, 1037, 1220, 1817
NaeI	GCCGGC	1 cut(s)	1706
NciI	CCSGG	2 cut(s)	1394, 1722
NcoI	CCATGG	2 cut(s)	615, 1227
NdeI	CATATG	2 cut(s)	796, 885
NgoMIV	GCCGGC	1 cut(s)	1704
NlaIII	CATG	7 cut(s)	619, 652, 779, 791, 1097, 1231, 1653
NlaIV	GGNNCC	6 cut(s)	350, 1062, 1233, 1638, 1757, 1839
NmeAIII	GCCGAG	1 cut(s)	1195
NmuCI	GTSAC	3 cut(s)	199, 1102, 1108
NsbI	TGCGCA	1 cut(s)	1487
NsiI	ATGCAT	2 cut(s)	777, 1000
NspI	RCATGY	2 cut(s)	779, 1653
PaeR7I	CTCGAG	1 cut(s)	1359
PaqCI	CACCTGC	1 cut(s)	1699
PciI	ACATGT	1 cut(s)	1649
PcsI	WCGNNNNNNNCGW	1 cut(s)	417
PctI	GAATGC	1 cut(s)	777
PdiI	GCCGGC	1 cut(s)	1706
PdmI	GAANNNNTTC	1 cut(s)	178
PfeI	GAWTC	2 cut(s)	1364, 1690
PkrI	GCNGC	5 cut(s)	529, 583, 586, 1039, 1879
PleI	GAGTC	4 cut(s)	989, 1508, 1532, 1562
PpsI	GAGTC	4 cut(s)	989, 1508, 1532, 1562
PpuMI	RGGWCCY	1 cut(s)	331
PscI	ACATGT	1 cut(s)	1649
PshAI	GACNNNNGTC	1 cut(s)	437
PshBI	ATTAAT	1 cut(s)	963
Psp124BI	GAGCTC	1 cut(s)	387
Psp5II	RGGWCCY	1 cut(s)	331
Psp6I	CCWGG	5 cut(s)	294, 324, 351, 849, 1545
PspFI	CCCAGC	2 cut(s)	802, 1285
PspGI	CCWGG	5 cut(s)	294, 324, 351, 849, 1545
PspN4I	GGNNCC	6 cut(s)	350, 1062, 1233, 1638, 1757, 1839
PspPI	GGNCC	2 cut(s)	322, 331
PspPPI	RGGWCCY	1 cut(s)	331
PstI	CTGCAG	2 cut(s)	525, 868
PstNI	CAGNNNCTG	1 cut(s)	1592
PsuI	RGATCY	2 cut(s)	396, 517
PvuII	CAGCTG	1 cut(s)	806
RsaI	GTAC	5 cut(s)	222, 365, 1132, 1302, 1349
RsaNI	GTAC	5 cut(s)	221, 364, 1131, 1301, 1348
RseI	CAYNNNNRTG	3 cut(s)	168, 614, 1098
SacI	GAGCTC	1 cut(s)	387
SaqAI	TTAA	4 cut(s)	555, 918, 963, 1902
SatI	GCNGC	5 cut(s)	528, 582, 585, 1038, 1878
Sau96I	GGNCC	2 cut(s)	322, 331
SchI	GAGTC	4 cut(s)	990, 1508, 1532, 1562
ScrFI	CCNGG	7 cut(s)	296, 326, 353, 851, 1394, 1547, 1722
SduI	GDGCHC	5 cut(s)	387, 594, 817, 1012, 1554
SfaNI	GCATC	5 cut(s)	533, 631, 966, 985, 1422
SfcI	CTRYAG	3 cut(s)	521, 864, 1907
Sfr274I	CTCGAG	1 cut(s)	1359
SinI	GGWCC	1 cut(s)	331
SlaI	CTCGAG	1 cut(s)	1359
SmiMI	CAYNNNNRTG	3 cut(s)	168, 614, 1098
SmlI	CTYRAG	2 cut(s)	1359, 1901
SmoI	CTYRAG	2 cut(s)	1359, 1901
Sse9I	AATT	5 cut(s)	1158, 1304, 1326, 1574, 1863
SsiI	CCGC	5 cut(s)	320, 429, 527, 585, 1020
SspI	AATATT	1 cut(s)	1747
SspMI	CTAG	5 cut(s)	621, 750, 1128, 1383, 1448
SstI	GAGCTC	1 cut(s)	387
StyD4I	CCNGG	7 cut(s)	294, 324, 351, 849, 1392, 1545, 1720
StyI	CCWWGG	2 cut(s)	615, 1227
TaaI	ACNGT	4 cut(s)	220, 225, 577, 1108
TaiI	ACGT	3 cut(s)	757, 932, 1677
TaqI	TCGA	6 cut(s)	256, 456, 464, 846, 1251, 1360
TasI	AATT	5 cut(s)	1158, 1304, 1326, 1574, 1863
TatI	WGTACW	3 cut(s)	1130, 1300, 1347
TauI	GCSGC	2 cut(s)	530, 587
TfiI	GAWTC	2 cut(s)	1364, 1690
Tru1I	TTAA	4 cut(s)	555, 918, 963, 1902
Tru9I	TTAA	4 cut(s)	555, 918, 963, 1902
TscAI	CASTG	1 cut(s)	868
TseFI	GTSAC	3 cut(s)	199, 1102, 1108
TseI	GCWGC	3 cut(s)	581, 1037, 1877
Tsp45I	GTSAC	3 cut(s)	199, 1102, 1108
TspDTI	ATGAA	4 cut(s)	665, 956, 1005, 1429
TspGWI	ACGGA	1 cut(s)	548
TspRI	CASTG	1 cut(s)	868
Vha464I	CTTAAG	1 cut(s)	1901
VneI	GTGCAC	1 cut(s)	1550
VpaK11BI	GGWCC	1 cut(s)	331
VspI	ATTAAT	1 cut(s)	963
XapI	RAATTY	1 cut(s)	1574
XceI	RCATGY	2 cut(s)	779, 1653
XhoI	CTCGAG	1 cut(s)	1359
XmnI	GAANNNNTTC	1 cut(s)	178
XspI	CTAG	5 cut(s)	621, 750, 1128, 1383, 1448
Zsp2I	ATGCAT	2 cut(s)	777, 1000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.