FvH4_6g40120

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012642
Physical Location & Seq
Reverse (-)
31682498 .. 31687925
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40120.t1

Sequence Viewer

Length: 1590 bp
ATGGAGATTTCAGCTCCTGAGGTTCTACATGAAGATGAACCAGTCTCTAACAAAACCCAGATTTGTAATCAAGAGAAAAGTAATGAAGAAATTTCCACGGTTCAACAGCCTCGAAGGCCAAACCTCTCCTCTCTGCAGATACCAGAGAGGACTCTGGAAAGTAGTTTATCTGCTTTTACGACGATTGATATTCCGTCTGGACAAAGTCCTAGTTCCACTAGAGTTGGGCTGCCGCCTAGACCACATTCAGCTAAGATCAAATCATCCGTGAAATATCTATTTCCTCAAAGGAGCTTTAGGGGGAAAAATATGACCCATGATGGTGAGAAGACCATTCTCATTCTACCAGATACAGCTTCATCAGATGCTTCTTTGGAGAAGCCTTCTACCTCGAGGTCTTTCTCACTTAATAGGATGTTCTTCTCTCCATCTTTAAAAGCAACACATTCTTTACCAGTTACACCGAGTGCAAATTCAGGTTCTGAGTCTGTGCAGGATAGACAGTCGGAAAGTCATTCTGATTTTTCTAATGAAGTTCAGCAGCATATGATCCGCTCATTTTCGGTTCCAGTAAATGTTAAAACCAGAAGTTTAAGGCGGATGGATTCTGGAGGCATGATGCGTGTAATTTCAACTCCACGTCCTGCAGTAGTTGATGCTACTTCCCCAAATAGTGCCCCAACAACGGATACTGCTACTGAAGATACTGGTGAGGATATTCCTGAAGAAGAAGCCGTTTGCCGAATTTGTTTGGTTGAGCTCGCAGAAGGTGGTGATACTCTTAAAATGGAGTGCAGCTGCAAAGGAGAGCTGGCACTGGCTCACAAAGATTGTGCAGTAAAGTGGTTTACCATTAAAGGTAACAAGACCTGTGATGTCTGCAGACAGGATGTTCGAAACCTACATGTAACACTACTTAAAATACACAATCCTCAAACTATTGTAAGACGACCACCAACAATACCCGAGCAGAGGGAAGTCGCTCGTTACAGGGTCTGGCAGGACATACCAGTGCTTGTTCTGGTCAGCATGCTTGCATATTTCTGCTTTCTGGAACAACTTCTGGTATCTGACTTGGGACCTCGTGCTCTTGCCATTTCCTTACCTTTCTCTTGTGTTTTAGGACTCCTTTCATCCATGATTGCTTCAACAATGGTAAGCAGGAGCTACATATGGGCCTATGCCTCCTTCCAGTTTGCCGTCGTGATCCTCTTTGCTCACATATTTTATACCATCCTTAATGTAAATCCAATTCTCTCGGTCATCCTTTCTGCATTTACTGGCTTTGGGATAGCGATCAGCACAAATTCTCTTCTAGTGGAGTATCTTAGATGGAAAGCAAGCAGGCAACTGGGATCTTCCCATACTAGCACTATGAATGAACTGCAATTGCAGCACCAGCAGTGGCAGCATGAAGAGCAATATTATCGCCATTACCAACAACAACGACGGCAACGATTGAGAGAGGAGTCCCAGAATCAGGTACAACAGCAAGAACAACAGCATCAACAGCAGCAGCACCAACACCAATTGAATGAAGAACCAAATGCGAATGGTTCCTTGCGGATAGACATGGGACGGGAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

530

Amino Acids

59.63

Weight (kDa)

6.79

Isoelectric Point (pI)

61.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 247 - 294 7.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 555
AciI CCGC 4 cut(s) 233, 553, 598, 1564
AclWI GGATC 3 cut(s) 544, 1201, 1363
AcsI RAATTY 4 cut(s) 90, 472, 744, 1306
AcuI CTGAAG 2 cut(s) 720, 744
AdeI CACNNNGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 1485
AfiI CCNNNNNNNGG 3 cut(s) 685, 972, 1480
AflIII ACRYGT 1 cut(s) 904
AgsI TTSAA 4 cut(s) 104, 633, 1149, 1534
AjiI CACGTC 1 cut(s) 641
AluBI AGCT 8 cut(s) 14, 251, 294, 356, 760, 798, 811, 1167
AluI AGCT 8 cut(s) 14, 251, 294, 356, 760, 798, 811, 1167
Alw21I GWGCWC 2 cut(s) 762, 1090
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 3 cut(s) 544, 1201, 1363
AlwNI CAGNNNCTG 3 cut(s) 17, 482, 996
Ama87I CYCGRG 2 cut(s) 391, 965
AoxI GGCC 2 cut(s) 116, 1176
ApeKI GCWGC 8 cut(s) 229, 541, 795, 798, 1393, 1408, 1513, 1516
ApoI RAATTY 4 cut(s) 90, 472, 744, 1306
ArsI GACNNNNNNTTYG 2 cut(s) 876, 908
Asp700I GAANNNNTTC 1 cut(s) 1059
AspS9I GGNCC 2 cut(s) 1079, 1176
AsuHPI GGTGA 3 cut(s) 335, 722, 785
AsuII TTCGAA 1 cut(s) 895
AvaI CYCGRG 2 cut(s) 391, 965
AvaII GGWCC 1 cut(s) 1079
AxyI CCTNAGG 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 762
BauI CACGAG 1 cut(s) 1083
BbsI GAAGAC 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 762, 1090
BbvI GCAGC 8 cut(s) 216, 553, 785, 807, 1405, 1420, 1525, 1528
BccI CCATC 5 cut(s) 314, 436, 595, 1241, 1326
BceAI ACGGC 3 cut(s) 719, 1184, 1466
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciVI GTATCC 1 cut(s) 682
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 5 cut(s) 210, 219, 237, 1316, 1368
BfmI CTRYAG 3 cut(s) 134, 645, 880
BfuI GTATCC 1 cut(s) 682
BglI GCCNNNNNGGC 1 cut(s) 115
BisI GCNGC 9 cut(s) 230, 233, 542, 796, 799, 1394, 1409, 1514, 1517
BlsI GCNGC 9 cut(s) 231, 234, 543, 797, 800, 1395, 1410, 1515, 1518
Bme18I GGWCC 1 cut(s) 1079
BmeT110I CYCGRG 2 cut(s) 391, 965
BmgBI CACGTC 1 cut(s) 641
BmgT120I GGNCC 2 cut(s) 1079, 1176
BmiI GGNNCC 3 cut(s) 567, 1080, 1558
BmrI ACTGGG 1 cut(s) 1361
BmsI GCATC 4 cut(s) 355, 609, 646, 1513
BmuI ACTGGG 1 cut(s) 1361
BpiI GAAGAC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 895
BsaBI GATNNNNATC 1 cut(s) 1295
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 972, 1480
Bse1I ACTGG 9 cut(s) 41, 455, 569, 712, 822, 1010, 1192, 1285, 1356
Bse21I CCTNAGG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 1295
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 7 cut(s) 263, 420, 606, 895, 1133, 1233, 1263
BseJI GATNNNNATC 1 cut(s) 1295
BseLI CCNNNNNNNGG 3 cut(s) 685, 972, 1480
BseMII CTCAG 2 cut(s) 9, 474
BseNI ACTGG 9 cut(s) 41, 455, 569, 712, 822, 1010, 1192, 1285, 1356
BseRI GAGGAG 2 cut(s) 118, 1481
BseSI GKGCMC 1 cut(s) 679
BseXI GCAGC 8 cut(s) 216, 553, 785, 807, 1405, 1420, 1525, 1528
BsgI GTGCAG 3 cut(s) 512, 814, 855
BshFI GGCC 2 cut(s) 118, 1178
BsiHKAI GWGCWC 2 cut(s) 762, 1090
BsiHKCI CYCGRG 2 cut(s) 391, 965
BslFI GGGAC 2 cut(s) 1092, 1456
BslI CCNNNNNNNGG 3 cut(s) 685, 972, 1480
BsmAI GTCTC 1 cut(s) 49
BsmFI GGGAC 2 cut(s) 1092, 1456
BsnI GGCC 2 cut(s) 118, 1178
BsoBI CYCGRG 2 cut(s) 391, 965
Bsp119I TTCGAA 1 cut(s) 895
Bsp1286I GDGCHC 3 cut(s) 679, 762, 1090
Bsp143I GATC 5 cut(s) 255, 549, 1206, 1296, 1355
BspACI CCGC 4 cut(s) 233, 553, 598, 1564
BspANI GGCC 2 cut(s) 118, 1178
BspCNI CTCAG 2 cut(s) 10, 475
BspLI GGNNCC 3 cut(s) 567, 1080, 1558
BspMAI CTGCAG 3 cut(s) 138, 649, 884
BspPI GGATC 3 cut(s) 544, 1201, 1363
BspQI GCTCTTC 1 cut(s) 1410
BspT104I TTCGAA 1 cut(s) 895
BsrBI CCGCTC 1 cut(s) 555
BsrI ACTGG 9 cut(s) 41, 455, 569, 712, 822, 1010, 1192, 1285, 1356
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 5 cut(s) 255, 549, 1206, 1296, 1355
BssSI CACGAG 1 cut(s) 1083
Bst2BI CACGAG 1 cut(s) 1083
Bst4CI ACNGT 2 cut(s) 100, 504
Bst6I CTCTTC 2 cut(s) 1317, 1410
BstBI TTCGAA 1 cut(s) 895
BstC8I GCNNGC 6 cut(s) 762, 813, 1031, 1035, 1342, 1346
BstDEI CTNAG 4 cut(s) 18, 252, 483, 1328
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 7 cut(s) 263, 420, 606, 895, 1133, 1233, 1263
BstKTI GATC 5 cut(s) 258, 552, 1209, 1299, 1358
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 5 cut(s) 255, 549, 1206, 1296, 1355
BstMWI GCNNNNNNNGC 6 cut(s) 115, 1393, 1399, 1408, 1417, 1510
BstNSI RCATGY 2 cut(s) 908, 1033
BstSFI CTRYAG 3 cut(s) 134, 645, 880
BstSLI GKGCMC 1 cut(s) 679
BstV1I GCAGC 8 cut(s) 216, 553, 785, 807, 1405, 1420, 1525, 1528
BstV2I GAAGAC 1 cut(s) 335
BstX2I RGATCY 1 cut(s) 1355
BstYI RGATCY 1 cut(s) 1355
Bsu36I CCTNAGG 1 cut(s) 18
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 2 cut(s) 118, 1178
BtgI CCRYGG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 641
BtsCI GGATG 7 cut(s) 263, 420, 606, 895, 1133, 1233, 1263
BtsI GCAGTG 1 cut(s) 1409
BtsIMutI CAGTG 3 cut(s) 815, 1017, 1409
Cac8I GCNNGC 6 cut(s) 762, 813, 1031, 1035, 1342, 1346
CaiI CAGNNNCTG 3 cut(s) 17, 482, 996
Cfr13I GGNCC 2 cut(s) 1079, 1176
Csp6I GTAC 1 cut(s) 1484
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviAII CATG 8 cut(s) 29, 317, 616, 905, 1030, 1138, 1412, 1573
CviQI GTAC 1 cut(s) 1484
DdeI CTNAG 4 cut(s) 18, 252, 483, 1328
DpnI GATC 5 cut(s) 257, 551, 1208, 1298, 1357
DpnII GATC 5 cut(s) 255, 549, 1206, 1296, 1355
DraI TTTAAA 1 cut(s) 435
DraIII CACNNNGTG 1 cut(s) 467
Eam1104I CTCTTC 2 cut(s) 1317, 1410
EarI CTCTTC 2 cut(s) 1317, 1410
EciI GGCGGA 1 cut(s) 613
Ecl136II GAGCTC 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 1079
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 720, 744
Eco81I CCTNAGG 1 cut(s) 18
Eco88I CYCGRG 2 cut(s) 391, 965
EcoICRI GAGCTC 1 cut(s) 760
EcoO109I RGGNCCY 1 cut(s) 1079
EcoT38I GRGCYC 1 cut(s) 762
FaeI CATG 8 cut(s) 32, 320, 619, 908, 1033, 1141, 1415, 1576
FaqI GGGAC 2 cut(s) 1092, 1456
FatI CATG 8 cut(s) 28, 316, 615, 904, 1029, 1137, 1411, 1572
FauNDI CATATG 2 cut(s) 546, 1172
Fnu4HI GCNGC 9 cut(s) 230, 233, 542, 796, 799, 1394, 1409, 1514, 1517
FokI GGATG 7 cut(s) 250, 427, 613, 902, 1120, 1220, 1250
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 9 cut(s) 230, 233, 542, 796, 799, 1394, 1409, 1514, 1517
FspBI CTAG 5 cut(s) 210, 219, 237, 1316, 1368
GluI GCNGC 9 cut(s) 230, 233, 542, 796, 799, 1394, 1409, 1514, 1517
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 118, 1178
Hin1II CATG 8 cut(s) 32, 320, 619, 908, 1033, 1141, 1415, 1576
HinfI GANTC 6 cut(s) 151, 485, 605, 1125, 1469, 1477
HphI GGTGA 3 cut(s) 335, 722, 785
Hpy166II GTNNAC 1 cut(s) 849
Hpy188I TCNGA 5 cut(s) 364, 484, 508, 520, 1072
Hpy188III TCNNGA 8 cut(s) 17, 71, 155, 198, 609, 722, 1052, 1204
Hpy8I GTNNAC 1 cut(s) 849
Hpy99I CGWCG 3 cut(s) 184, 1205, 1452
HpyAV CCTTC 4 cut(s) 108, 393, 761, 1198
HpyCH4III ACNGT 2 cut(s) 100, 504
HpyCH4IV ACGT 1 cut(s) 640
HpyF10VI GCNNNNNNNGC 6 cut(s) 115, 1393, 1399, 1408, 1417, 1510
HpyF3I CTNAG 4 cut(s) 18, 252, 483, 1328
HpySE526I ACGT 1 cut(s) 640
Hsp92II CATG 8 cut(s) 32, 320, 619, 908, 1033, 1141, 1415, 1576
Kzo9I GATC 5 cut(s) 255, 549, 1206, 1296, 1355
LguI GCTCTTC 1 cut(s) 1410
LmnI GCTCC 3 cut(s) 19, 291, 1164
Lsp1109I GCAGC 8 cut(s) 216, 553, 785, 807, 1405, 1420, 1525, 1528
LweI GCATC 4 cut(s) 355, 609, 646, 1513
MaeI CTAG 5 cut(s) 210, 219, 237, 1316, 1368
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 4 cut(s) 457, 860, 907, 986
MalI GATC 5 cut(s) 257, 551, 1208, 1298, 1357
MbiI CCGCTC 1 cut(s) 555
MboI GATC 5 cut(s) 255, 549, 1206, 1296, 1355
MfeI CAATTG 2 cut(s) 1388, 1529
MflI RGATCY 1 cut(s) 1355
MhlI GDGCHC 3 cut(s) 679, 762, 1090
MluCI AATT 8 cut(s) 90, 472, 627, 744, 1251, 1306, 1388, 1529
MlyI GAGTC 4 cut(s) 145, 494, 1119, 1478
MmeI TCCRAC 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 1059
MseI TTAA 8 cut(s) 408, 434, 579, 593, 783, 855, 918, 1239
MslI CAYNNNNRTG 3 cut(s) 33, 321, 1010
MspA1I CMGCKG 1 cut(s) 798
MunI CAATTG 2 cut(s) 1388, 1529
MwoI GCNNNNNNNGC 6 cut(s) 115, 1393, 1399, 1408, 1417, 1510
NdeI CATATG 2 cut(s) 546, 1172
NdeII GATC 5 cut(s) 255, 549, 1206, 1296, 1355
NlaIII CATG 8 cut(s) 32, 320, 619, 908, 1033, 1141, 1415, 1576
NlaIV GGNNCC 3 cut(s) 567, 1080, 1558
NspI RCATGY 2 cut(s) 908, 1033
NspV TTCGAA 1 cut(s) 895
PaeI GCATGC 1 cut(s) 1033
PaeR7I CTCGAG 1 cut(s) 391
PciI ACATGT 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 1410
PcsI WCGNNNNNNNCGW 1 cut(s) 1453
PdmI GAANNNNTTC 1 cut(s) 1059
PfeI GAWTC 2 cut(s) 605, 1477
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 9 cut(s) 231, 234, 543, 797, 800, 1395, 1410, 1515, 1518
PleI GAGTC 4 cut(s) 145, 493, 1119, 1477
PpsI GAGTC 4 cut(s) 145, 493, 1119, 1477
PpuMI RGGWCCY 1 cut(s) 1079
PscI ACATGT 1 cut(s) 904
Psp124BI GAGCTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 1079
PspN4I GGNNCC 3 cut(s) 567, 1080, 1558
PspPI GGNCC 2 cut(s) 1079, 1176
PspPPI RGGWCCY 1 cut(s) 1079
PspXI VCTCGAGB 1 cut(s) 391
PstI CTGCAG 3 cut(s) 138, 649, 884
PstNI CAGNNNCTG 3 cut(s) 17, 482, 996
PsuI RGATCY 1 cut(s) 1355
PsyI GACNNNGTC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 798
RsaI GTAC 1 cut(s) 1485
RsaNI GTAC 1 cut(s) 1484
RseI CAYNNNNRTG 3 cut(s) 33, 321, 1010
SacI GAGCTC 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 1410
SaqAI TTAA 8 cut(s) 408, 434, 579, 593, 783, 855, 918, 1239
SatI GCNGC 9 cut(s) 230, 233, 542, 796, 799, 1394, 1409, 1514, 1517
Sau3AI GATC 5 cut(s) 255, 549, 1206, 1296, 1355
Sau96I GGNCC 2 cut(s) 1079, 1176
SchI GAGTC 4 cut(s) 145, 494, 1119, 1478
SduI GDGCHC 3 cut(s) 679, 762, 1090
SfaNI GCATC 4 cut(s) 355, 609, 646, 1513
SfcI CTRYAG 3 cut(s) 134, 645, 880
Sfr274I CTCGAG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 895
SinI GGWCC 1 cut(s) 1079
SlaI CTCGAG 1 cut(s) 391
SmiMI CAYNNNNRTG 3 cut(s) 33, 321, 1010
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SphI GCATGC 1 cut(s) 1033
Sse9I AATT 8 cut(s) 90, 472, 627, 744, 1251, 1306, 1388, 1529
SsiI CCGC 4 cut(s) 233, 553, 598, 1564
SspI AATATT 1 cut(s) 1424
SspMI CTAG 5 cut(s) 210, 219, 237, 1316, 1368
SstI GAGCTC 1 cut(s) 762
TaaI ACNGT 2 cut(s) 100, 504
TaiI ACGT 1 cut(s) 643
TaqI TCGA 3 cut(s) 112, 392, 895
TaqII GACCGA 1 cut(s) 1249
TasI AATT 8 cut(s) 90, 472, 627, 744, 1251, 1306, 1388, 1529
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 2 cut(s) 605, 1477
Tru1I TTAA 8 cut(s) 408, 434, 579, 593, 783, 855, 918, 1239
Tru9I TTAA 8 cut(s) 408, 434, 579, 593, 783, 855, 918, 1239
TscAI CASTG 3 cut(s) 822, 1017, 1409
TseI GCWGC 8 cut(s) 229, 541, 795, 798, 1393, 1408, 1513, 1516
TspGWI ACGGA 3 cut(s) 183, 256, 701
TspRI CASTG 3 cut(s) 822, 1017, 1409
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 1079
XapI RAATTY 4 cut(s) 90, 472, 744, 1306
XceI RCATGY 2 cut(s) 908, 1033
XhoI CTCGAG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 1059
XspI CTAG 5 cut(s) 210, 219, 237, 1316, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.