Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012642

Physical Location & Seq

Genomic Coordinates Forward (+)

10375605 .. 10380996

Gene Structure 5392 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1135100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGGCGGAGAATTCAGCTTCTGAGCTTCAGCTCGGAGAGGAATCAACTCCGGAGAGCTCATCTGATCAGCACCAGATTCATGAACAAAAGGAAAAGAATGAAGAAACCTCCTTGGTGCAGCAGTCTAGACGGCCAAACCTCTCCTCATTGCAAATACCGGCAAGGACATTGGAAACCAGTTTGTCTGCTTGTACGAGAATTGATATTCCCTCCACAGCAAGTCCAAGTTCTAGTAGAGTTGGACTACCGCCTAGGCCACATTCAGCTAAGATCAAATCAACCGTGAAAAATCTGTTTCCTCAAAAAAGTTTTCGGGTGAAAAACTTGCCCCTGGATGGCGAGAAGACGATTCTCATTATGCCGGATACATCGCCCTCAGATAGTCCGTTGGAAAAGCCTTCTACTTCACGGTCTTTCTCGCTCAATAAGCTTTTCTTCTCTACATCAACAAAAGGGACGCATTCTTTACCAGTTACACCAAGTGCAAATCTGGGTTCCGAGATTGGGCAGGGAAGACAAGTGGAGAGTCATTCTGATTCTAGAATTGAAGTTAAGCATCATATGTCCCGCTCTTTCTCAGTTCCAGTAAATATTGCGACCAGAAGTTTAAGGCGGATGGATTCTGGATGCATGATCCGTGTAATTTCAGCAACTCCACGTCCTTCAGCAGTTGAGGGTGCCTCACCAAATGCTGACCCAGCACTGGAAATGGCCACTGAAGATTCGGGTGAAGATATTCCTGAAGAAGAAGCAGTTTGCAGGATTTGTTTGGTTGAGCTCGCAGAAGGTGGTGATTCTCTTAAAATGGAGTGCAGCTGCAAAGGAGAGCTAGCACTTGCTCACAAAGATTGTGCAGTAAAGTGGTTTAGCATTAAGGGGAACAAAACCTGTGACGTCTGCAAGCAGGATGTCCTAAACTTACCCGTAACACTGCTTAAAATACACAATCCTCAAACTGTTATCAGACGACCACCAACAATAATGGAGCAGAGGGAAGTCCATCATTACAGGGTCTGGCAGGACATACCGGTTCTTGTCCTGGTCAGCATGCTTGCGTATTTCTGCTTTCTGGAGCAACTTCTGGTTTGTTTTGTGCTTATTTACAATAAACTCAAGCTCTTCATTATATGGAATCATGGTTTTGGTAGCAAGCTCATTCTTGCTGGCAAAGAGACAATTCTAGATTTCATAAGTCGCAGCAATTGTTCAGTCATACTTCATATTTTCTGTGATATGATCCTGAAGAATTTACAACTTGCAGTCTATACATCTATGCTTGTATCTGACTTGGGCCCTCGTGCTCTTGCCGTTTCCTTACCCTTCTCTTGTGTTTTAGGTCTCCTTTCATCTATGATTGCTTCCACAATGGTGAGCAGGAGCTACTTATGGGCTTATGCCTCCTTCCAGTTTGCCATTGTGATCCTGTTTGCTCACATATTTTATACTGTACTTAATGTAAATCCAATTCTCTCAGTCCTCCTTTCCTCATTCACTGGCTTCGGGATTGCAATCAGCACGAATTCCCTTCTGGTTGAGTATCGTAGATGGAAAATCAGCAGACAACTGCAATCATCCTATCAACATGCTAGCAGTGCACTGCATCTGCATGAACTACAACTGCAGCAGCACCGGCAACAGCAAGAAGAGCAATATCAACACCAATCCCAGCAACAACGACGGCAACAGTTGATGGAAGATTCATATAATCAGTACCAGCAAGTACAGCGACAGCTTCAGCAGCAAGAAGAAGAACATCATCGACACCAACAGGAGCACCAGCACCAGCGGTTGGATGGAGATCCAAATGCTGATGGTTCTAGGCCGCTAGAAGTTAGACAGGGCACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RINGv

Protein Analysis

616

Amino Acids

69.2

Weight (kDa)

7.1

Isoelectric Point (pI)

69.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RINGv	PF12906	253 - 300	8.2e-13	RING-variant domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012642)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40120
malus_domestica	MD09G1135100.v1.1 MD17G1124100.v1.1
prunus_persica	Prupe.3G191600_v2.0.a1 Prupe.3G191600_v2.0.a1 Prupe.3G191600_v2.0.a1
pyrus_communis	pycom09g05620 pycom17g11550
rosa_chinensis	RchiOBHm_Chr2g0154751
rosa_laevigata	RLG00000020800
rosa_multiflora	Rmu_sc0001004.1_g000002 Rmu_sc0001027.1_g000003
rosa_roxburghii	Rroxscaffold_2G00094130
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2AG510400 Rh2BG521300 Rh2CG495600 Rh2DG531400
rosa_wichuraiana	Rw2G041980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	897
AccB1I	GGYRCC	1 cut(s)	677
AccB7I	CCANNNNNTGG	1 cut(s)	1789
AccBSI	CCGCTC	1 cut(s)	570
AccIII	TCCGGA	1 cut(s)	49
AciI	CCGC	6 cut(s)	5, 248, 568, 613, 1786, 1823
AclWI	GGATC	4 cut(s)	628, 1231, 1414, 1793
AcoI	YGGCCR	2 cut(s)	131, 711
AcsI	RAATTY	3 cut(s)	10, 1246, 1519
AcuI	CTGAAG	6 cut(s)	11, 648, 738, 762, 1262, 1718
AcyI	GRCGYC	1 cut(s)	894
AdeI	CACNNNGTG	1 cut(s)	482
AfaI	GTAC	4 cut(s)	193, 1449, 1712, 1722
AfiI	CCNNNNNNNGG	4 cut(s)	335, 504, 703, 1789
AgeI	ACCGGT	1 cut(s)	1027
AgsI	TTSAA	1 cut(s)	548
AjiI	CACGTC	1 cut(s)	659
AjnI	CCWGG	2 cut(s)	330, 1038
AleI	CACNNNNGTG	1 cut(s)	1367
Alw21I	GWGCWC	5 cut(s)	59, 780, 1303, 1597, 1776
Alw26I	GTCTC	2 cut(s)	1166, 1343
Alw44I	GTGCAC	1 cut(s)	1593
AlwI	GGATC	4 cut(s)	628, 1231, 1414, 1793
AlwNI	CAGNNNCTG	2 cut(s)	20, 1014
Aor13HI	TCCGGA	1 cut(s)	49
AoxI	GGCC	5 cut(s)	131, 254, 711, 1291, 1820
ApaI	GGGCCC	1 cut(s)	1295
ApaLI	GTGCAC	1 cut(s)	1593
ApeKI	GCWGC	7 cut(s)	118, 813, 816, 1197, 1621, 1624, 1738
ApoI	RAATTY	3 cut(s)	10, 1246, 1519
AsiGI	ACCGGT	1 cut(s)	1027
Asp700I	GAANNNNTTC	1 cut(s)	735
AspA2I	CCTAGG	1 cut(s)	251
AspS9I	GGNCC	2 cut(s)	1291, 1292
AsuHPI	GGTGA	5 cut(s)	328, 675, 740, 803, 1381
AsuNHI	GCTAGC	2 cut(s)	829, 1586
AvrII	CCTAGG	1 cut(s)	251
BaeGI	GKGCMC	3 cut(s)	1295, 1597, 1844
BalI	TGGCCA	1 cut(s)	713
BanI	GGYRCC	1 cut(s)	677
BanII	GRGCYC	3 cut(s)	59, 780, 1295
BauI	CACGAG	1 cut(s)	1296
BbsI	GAAGAC	2 cut(s)	350, 520
Bbv12I	GWGCWC	5 cut(s)	59, 780, 1303, 1597, 1776
BbvI	GCAGC	7 cut(s)	130, 803, 825, 1209, 1633, 1636, 1750
BccI	CCATC	7 cut(s)	329, 610, 1008, 1539, 1684, 1787, 1805
BceAI	ACGGC	3 cut(s)	146, 1292, 1694
BciT130I	CCWGG	2 cut(s)	332, 1040
BciVI	GTATCC	1 cut(s)	358
BclI	TGATCA	1 cut(s)	64
BcoDI	GTCTC	2 cut(s)	1166, 1343
BfaI	CTAG	9 cut(s)	126, 231, 252, 540, 830, 1181, 1587, 1818, 1826
BfmI	CTRYAG	1 cut(s)	1619
BfuI	GTATCC	1 cut(s)	358
BisI	GCNGC	8 cut(s)	119, 814, 817, 1198, 1622, 1625, 1739, 1823
BlnI	CCTAGG	1 cut(s)	251
BlsI	GCNGC	8 cut(s)	120, 815, 818, 1199, 1623, 1626, 1740, 1824
Bme1390I	CCNGG	2 cut(s)	332, 1040
BmgBI	CACGTC	1 cut(s)	659
BmgT120I	GGNCC	2 cut(s)	1291, 1292
BmiI	GGNNCC	3 cut(s)	496, 679, 1293
BmrFI	CCNGG	2 cut(s)	332, 1040
BmsI	GCATC	3 cut(s)	565, 617, 1609
BmtI	GCTAGC	2 cut(s)	833, 1590
BpiI	GAAGAC	2 cut(s)	350, 520
BplI	GAGNNNNNCTC	2 cut(s)	665, 697
BpmI	CTGGAG	1 cut(s)	1091
BpuEI	CTTGAG	1 cut(s)	1097
BsaBI	GATNNNNATC	2 cut(s)	1508, 1797
BsaHI	GRCGYC	1 cut(s)	894
BsaI	GGTCTC	1 cut(s)	1343
BsaJI	CCNNGG	3 cut(s)	111, 251, 330
BsaWI	WCCGGW	2 cut(s)	49, 1027
Bsc4I	CCNNNNNNNGG	4 cut(s)	335, 504, 703, 1789
Bse118I	RCCGGY	3 cut(s)	157, 1027, 1629
Bse1I	ACTGG	6 cut(s)	177, 470, 584, 708, 1405, 1498
Bse3DI	GCAATG	1 cut(s)	146
Bse8I	GATNNNNATC	2 cut(s)	1508, 1797
BseAI	TCCGGA	1 cut(s)	49
BseBI	CCWGG	2 cut(s)	332, 1040
BseDI	CCNNGG	3 cut(s)	111, 251, 330
BseGI	GGATG	6 cut(s)	340, 621, 632, 913, 1571, 1798
BseJI	GATNNNNATC	2 cut(s)	1508, 1797
BseLI	CCNNNNNNNGG	4 cut(s)	335, 504, 703, 1789
BseMI	GCAATG	1 cut(s)	146
BseMII	CTCAG	4 cut(s)	12, 390, 591, 1485
BseNI	ACTGG	6 cut(s)	177, 470, 584, 708, 1405, 1498
BseRI	GAGGAG	1 cut(s)	133
BseSI	GKGCMC	3 cut(s)	1295, 1597, 1844
BseXI	GCAGC	7 cut(s)	130, 803, 825, 1209, 1633, 1636, 1750
BseYI	CCCAGC	2 cut(s)	697, 1665
BsgI	GTGCAG	3 cut(s)	137, 832, 873
BshFI	GGCC	5 cut(s)	133, 256, 713, 1293, 1822
BshNI	GGYRCC	1 cut(s)	677
BshTI	ACCGGT	1 cut(s)	1027
BsiHKAI	GWGCWC	5 cut(s)	59, 780, 1303, 1597, 1776
BsiSI	CCGG	5 cut(s)	50, 158, 362, 1028, 1630
BslFI	GGGAC	2 cut(s)	469, 550
BslI	CCNNNNNNNGG	4 cut(s)	335, 504, 703, 1789
BsmAI	GTCTC	2 cut(s)	1166, 1343
BsmFI	GGGAC	2 cut(s)	469, 550
BsmI	GAATGC	1 cut(s)	460
BsnI	GGCC	5 cut(s)	133, 256, 713, 1293, 1822
Bso31I	GGTCTC	1 cut(s)	1343
Bsp120I	GGGCCC	1 cut(s)	1291
Bsp1286I	GDGCHC	7 cut(s)	59, 780, 1295, 1303, 1597, 1776, 1844
Bsp13I	TCCGGA	1 cut(s)	49
Bsp143I	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
BspACI	CCGC	6 cut(s)	5, 248, 568, 613, 1786, 1823
BspANI	GGCC	5 cut(s)	133, 256, 713, 1293, 1822
BspCNI	CTCAG	4 cut(s)	13, 389, 590, 1484
BspEI	TCCGGA	1 cut(s)	49
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	3 cut(s)	496, 679, 1293
BspMAI	CTGCAG	1 cut(s)	1623
BspOI	GCTAGC	2 cut(s)	833, 1590
BspPI	GGATC	4 cut(s)	628, 1231, 1414, 1793
BspQI	GCTCTTC	2 cut(s)	1124, 1638
BspT107I	GGYRCC	1 cut(s)	677
BspTNI	GGTCTC	1 cut(s)	1343
BsrBI	CCGCTC	1 cut(s)	570
BsrDI	GCAATG	1 cut(s)	146
BsrFI	RCCGGY	3 cut(s)	157, 1027, 1629
BsrI	ACTGG	6 cut(s)	177, 470, 584, 708, 1405, 1498
BssAI	RCCGGY	3 cut(s)	157, 1027, 1629
BssECI	CCNNGG	3 cut(s)	111, 251, 330
BssMI	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
BssNI	GRCGYC	1 cut(s)	894
BssSI	CACGAG	1 cut(s)	1296
BssT1I	CCWWGG	2 cut(s)	111, 251
Bst2BI	CACGAG	1 cut(s)	1296
Bst2UI	CCWGG	2 cut(s)	332, 1040
Bst4CI	ACNGT	5 cut(s)	283, 411, 958, 1447, 1686
Bst6I	CTCTTC	2 cut(s)	1124, 1638
BstACI	GRCGYC	1 cut(s)	894
BstC8I	GCNNGC	8 cut(s)	780, 831, 902, 1049, 1053, 1151, 1165, 1588
BstDEI	CTNAG	5 cut(s)	21, 267, 376, 577, 1471
BstF5I	GGATG	6 cut(s)	340, 621, 632, 913, 1571, 1798
BstKTI	GATC	6 cut(s)	67, 273, 636, 1239, 1422, 1801
BstMAI	GTCTC	2 cut(s)	1166, 1343
BstMBI	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
BstMWI	GCNNNNNNNGC	7 cut(s)	427, 698, 1592, 1630, 1645, 1723, 1738
BstNI	CCWGG	2 cut(s)	332, 1040
BstNSI	RCATGY	2 cut(s)	1051, 1586
BstSCI	CCNGG	2 cut(s)	330, 1038
BstSFI	CTRYAG	1 cut(s)	1619
BstSLI	GKGCMC	3 cut(s)	1295, 1597, 1844
BstV1I	GCAGC	7 cut(s)	130, 803, 825, 1209, 1633, 1636, 1750
BstV2I	GAAGAC	2 cut(s)	350, 520
BstX2I	RGATCY	1 cut(s)	1798
BstYI	RGATCY	1 cut(s)	1798
BsuI	GTATCC	1 cut(s)	358
BsuRI	GGCC	5 cut(s)	133, 256, 713, 1293, 1822
BtgZI	GCGATG	1 cut(s)	354
BtrI	CACGTC	1 cut(s)	659
BtsCI	GGATG	6 cut(s)	340, 621, 632, 913, 1571, 1798
BtsI	GCAGTG	3 cut(s)	929, 1595, 1597
BtsIMutI	CAGTG	6 cut(s)	701, 714, 929, 1491, 1595, 1597
Cac8I	GCNNGC	8 cut(s)	780, 831, 902, 1049, 1053, 1151, 1165, 1588
CaiI	CAGNNNCTG	2 cut(s)	20, 1014
CciI	TCATGA	1 cut(s)	79
Cfr10I	RCCGGY	3 cut(s)	157, 1027, 1629
Cfr13I	GGNCC	2 cut(s)	1291, 1292
CseI	GACGC	1 cut(s)	466
Csp6I	GTAC	4 cut(s)	192, 1448, 1711, 1721
CspAI	ACCGGT	1 cut(s)	1027
CviAII	CATG	6 cut(s)	80, 631, 1048, 1136, 1583, 1607
CviQI	GTAC	4 cut(s)	192, 1448, 1711, 1721
DdeI	CTNAG	5 cut(s)	21, 267, 376, 577, 1471
DpnI	GATC	6 cut(s)	66, 272, 635, 1238, 1421, 1800
DpnII	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
DraIII	CACNNNGTG	1 cut(s)	482
EaeI	YGGCCR	2 cut(s)	131, 711
Eam1104I	CTCTTC	2 cut(s)	1124, 1638
EarI	CTCTTC	2 cut(s)	1124, 1638
EciI	GGCGGA	2 cut(s)	20, 628
Ecl136II	GAGCTC	2 cut(s)	57, 778
Eco130I	CCWWGG	2 cut(s)	111, 251
Eco24I	GRGCYC	3 cut(s)	59, 780, 1295
Eco31I	GGTCTC	1 cut(s)	1343
Eco53kI	GAGCTC	2 cut(s)	57, 778
Eco57I	CTGAAG	6 cut(s)	11, 648, 738, 762, 1262, 1718
EcoICRI	GAGCTC	2 cut(s)	57, 778
EcoO109I	RGGNCCY	1 cut(s)	1292
EcoRI	GAATTC	2 cut(s)	10, 1519
EcoRII	CCWGG	2 cut(s)	330, 1038
EcoT14I	CCWWGG	2 cut(s)	111, 251
EcoT22I	ATGCAT	1 cut(s)	632
EcoT38I	GRGCYC	3 cut(s)	59, 780, 1295
ErhI	CCWWGG	2 cut(s)	111, 251
FaeI	CATG	6 cut(s)	83, 634, 1051, 1139, 1586, 1610
FalI	AAGNNNNNCTT	2 cut(s)	419, 451
FaqI	GGGAC	2 cut(s)	469, 550
FatI	CATG	6 cut(s)	79, 630, 1047, 1135, 1582, 1606
FauI	CCCGC	1 cut(s)	575
FauNDI	CATATG	1 cut(s)	561
FbaI	TGATCA	1 cut(s)	64
Fnu4HI	GCNGC	8 cut(s)	119, 814, 817, 1198, 1622, 1625, 1739, 1823
FokI	GGATG	6 cut(s)	347, 628, 639, 920, 1558, 1805
FriOI	GRGCYC	3 cut(s)	59, 780, 1295
Fsp4HI	GCNGC	8 cut(s)	119, 814, 817, 1198, 1622, 1625, 1739, 1823
FspBI	CTAG	9 cut(s)	126, 231, 252, 540, 830, 1181, 1587, 1818, 1826
GluI	GCNGC	8 cut(s)	119, 814, 817, 1198, 1622, 1625, 1739, 1823
GsaI	CCCAGC	2 cut(s)	701, 1669
GsuI	CTGGAG	1 cut(s)	1091
HaeIII	GGCC	5 cut(s)	133, 256, 713, 1293, 1822
HapII	CCGG	5 cut(s)	50, 158, 362, 1028, 1630
HgaI	GACGC	1 cut(s)	466
Hin1I	GRCGYC	1 cut(s)	894
Hin1II	CATG	6 cut(s)	83, 634, 1051, 1139, 1586, 1610
HindIII	AAGCTT	1 cut(s)	428
HpaII	CCGG	5 cut(s)	50, 158, 362, 1028, 1630
HphI	GGTGA	5 cut(s)	328, 675, 740, 803, 1381
Hpy166II	GTNNAC	1 cut(s)	1595
Hpy188I	TCNGA	8 cut(s)	22, 35, 64, 379, 499, 535, 965, 1285
Hpy8I	GTNNAC	1 cut(s)	1595
Hpy99I	CGWCG	1 cut(s)	1680
HpyAV	CCTTC	6 cut(s)	408, 672, 779, 1330, 1411, 1535
HpyCH4III	ACNGT	5 cut(s)	283, 411, 958, 1447, 1686
HpyCH4IV	ACGT	2 cut(s)	658, 894
HpyF10VI	GCNNNNNNNGC	7 cut(s)	427, 698, 1592, 1630, 1645, 1723, 1738
HpyF3I	CTNAG	5 cut(s)	21, 267, 376, 577, 1471
HpySE526I	ACGT	2 cut(s)	658, 894
Hsp92I	GRCGYC	1 cut(s)	894
Hsp92II	CATG	6 cut(s)	83, 634, 1051, 1139, 1586, 1610
Kpn2I	TCCGGA	1 cut(s)	49
Ksp22I	TGATCA	1 cut(s)	64
Kzo9I	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
LguI	GCTCTTC	2 cut(s)	1124, 1638
LmnI	GCTCC	4 cut(s)	985, 1072, 1377, 1771
Lsp1109I	GCAGC	7 cut(s)	130, 803, 825, 1209, 1633, 1636, 1750
LweI	GCATC	3 cut(s)	565, 617, 1609
MaeI	CTAG	9 cut(s)	126, 231, 252, 540, 830, 1181, 1587, 1818, 1826
MaeII	ACGT	2 cut(s)	658, 894
MaeIII	GTNAC	3 cut(s)	472, 890, 925
MalI	GATC	6 cut(s)	66, 272, 635, 1238, 1421, 1800
MbiI	CCGCTC	1 cut(s)	570
MboI	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
MfeI	CAATTG	1 cut(s)	1201
MflI	RGATCY	1 cut(s)	1798
MhlI	GDGCHC	7 cut(s)	59, 780, 1295, 1303, 1597, 1776, 1844
MlsI	TGGCCA	1 cut(s)	713
MluCI	AATT	9 cut(s)	10, 198, 543, 642, 1176, 1201, 1246, 1464, 1519
MluNI	TGGCCA	1 cut(s)	713
MlyI	GAGTC	1 cut(s)	535
MmeI	TCCRAC	3 cut(s)	220, 369, 1770
Mox20I	TGGCCA	1 cut(s)	713
Mph1103I	ATGCAT	1 cut(s)	632
MroI	TCCGGA	1 cut(s)	49
MroXI	GAANNNNTTC	1 cut(s)	735
MscI	TGGCCA	1 cut(s)	713
MseI	TTAA	6 cut(s)	552, 608, 801, 873, 936, 1452
MslI	CAYNNNNRTG	2 cut(s)	1367, 1605
Msp20I	TGGCCA	1 cut(s)	713
MspA1I	CMGCKG	2 cut(s)	816, 1786
MspI	CCGG	5 cut(s)	50, 158, 362, 1028, 1630
MspR9I	CCNGG	2 cut(s)	332, 1040
MunI	CAATTG	1 cut(s)	1201
Mva1269I	GAATGC	1 cut(s)	460
MvaI	CCWGG	2 cut(s)	332, 1040
MwoI	GCNNNNNNNGC	7 cut(s)	427, 698, 1592, 1630, 1645, 1723, 1738
NdeI	CATATG	1 cut(s)	561
NdeII	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
NheI	GCTAGC	2 cut(s)	829, 1586
NlaIII	CATG	6 cut(s)	83, 634, 1051, 1139, 1586, 1610
NlaIV	GGNNCC	3 cut(s)	496, 679, 1293
NmuCI	GTSAC	1 cut(s)	890
NsiI	ATGCAT	1 cut(s)	632
NspI	RCATGY	2 cut(s)	1051, 1586
OliI	CACNNNNGTG	1 cut(s)	1367
PaeI	GCATGC	1 cut(s)	1051
PagI	TCATGA	1 cut(s)	79
PciSI	GCTCTTC	2 cut(s)	1124, 1638
PctI	GAATGC	1 cut(s)	460
PdmI	GAANNNNTTC	1 cut(s)	735
PfeI	GAWTC	9 cut(s)	41, 76, 349, 536, 620, 722, 794, 1132, 1697
PflMI	CCANNNNNTGG	1 cut(s)	1789
PinAI	ACCGGT	1 cut(s)	1027
PkrI	GCNGC	8 cut(s)	120, 815, 818, 1199, 1623, 1626, 1740, 1824
PleI	GAGTC	1 cut(s)	534
PpsI	GAGTC	1 cut(s)	534
Psp124BI	GAGCTC	2 cut(s)	59, 780
Psp6I	CCWGG	2 cut(s)	330, 1038
PspFI	CCCAGC	2 cut(s)	697, 1665
PspGI	CCWGG	2 cut(s)	330, 1038
PspN4I	GGNNCC	3 cut(s)	496, 679, 1293
PspOMI	GGGCCC	1 cut(s)	1291
PspPI	GGNCC	2 cut(s)	1291, 1292
PstI	CTGCAG	1 cut(s)	1623
PstNI	CAGNNNCTG	2 cut(s)	20, 1014
PsuI	RGATCY	1 cut(s)	1798
PvuII	CAGCTG	1 cut(s)	816
RsaI	GTAC	4 cut(s)	193, 1449, 1712, 1722
RsaNI	GTAC	4 cut(s)	192, 1448, 1711, 1721
RseI	CAYNNNNRTG	2 cut(s)	1367, 1605
SacI	GAGCTC	2 cut(s)	59, 780
SapI	GCTCTTC	2 cut(s)	1124, 1638
SaqAI	TTAA	6 cut(s)	552, 608, 801, 873, 936, 1452
SatI	GCNGC	8 cut(s)	119, 814, 817, 1198, 1622, 1625, 1739, 1823
Sau3AI	GATC	6 cut(s)	64, 270, 633, 1236, 1419, 1798
Sau96I	GGNCC	2 cut(s)	1291, 1292
SchI	GAGTC	1 cut(s)	535
ScrFI	CCNGG	2 cut(s)	332, 1040
SduI	GDGCHC	7 cut(s)	59, 780, 1295, 1303, 1597, 1776, 1844
SfaNI	GCATC	3 cut(s)	565, 617, 1609
SfcI	CTRYAG	1 cut(s)	1619
SmiMI	CAYNNNNRTG	2 cut(s)	1367, 1605
SmlI	CTYRAG	1 cut(s)	1112
SmoI	CTYRAG	1 cut(s)	1112
SphI	GCATGC	1 cut(s)	1051
Sse9I	AATT	9 cut(s)	10, 198, 543, 642, 1176, 1201, 1246, 1464, 1519
SsiI	CCGC	6 cut(s)	5, 248, 568, 613, 1786, 1823
SspI	AATATT	1 cut(s)	592
SspMI	CTAG	9 cut(s)	126, 231, 252, 540, 830, 1181, 1587, 1818, 1826
SstI	GAGCTC	2 cut(s)	59, 780
StyD4I	CCNGG	2 cut(s)	330, 1038
StyI	CCWWGG	2 cut(s)	111, 251
TaaI	ACNGT	5 cut(s)	283, 411, 958, 1447, 1686
TaiI	ACGT	2 cut(s)	661, 897
TaqI	TCGA	1 cut(s)	1759
TasI	AATT	9 cut(s)	10, 198, 543, 642, 1176, 1201, 1246, 1464, 1519
TatI	WGTACW	2 cut(s)	1447, 1720
TauI	GCSGC	1 cut(s)	1825
TfiI	GAWTC	9 cut(s)	41, 76, 349, 536, 620, 722, 794, 1132, 1697
Tru1I	TTAA	6 cut(s)	552, 608, 801, 873, 936, 1452
Tru9I	TTAA	6 cut(s)	552, 608, 801, 873, 936, 1452
TscAI	CASTG	6 cut(s)	708, 721, 936, 1498, 1597, 1602
TseFI	GTSAC	1 cut(s)	890
TseI	GCWGC	7 cut(s)	118, 813, 816, 1197, 1621, 1624, 1738
Tsp45I	GTSAC	1 cut(s)	890
TspDTI	ATGAA	9 cut(s)	68, 96, 114, 1111, 1177, 1208, 1335, 1623, 1689
TspGWI	ACGGA	2 cut(s)	375, 626
TspRI	CASTG	6 cut(s)	708, 721, 936, 1498, 1597, 1602
Van91I	CCANNNNNTGG	1 cut(s)	1789
VneI	GTGCAC	1 cut(s)	1593
XapI	RAATTY	3 cut(s)	10, 1246, 1519
XbaI	TCTAGA	3 cut(s)	125, 539, 1180
XceI	RCATGY	2 cut(s)	1051, 1586
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1790
XmaJI	CCTAGG	1 cut(s)	251
XmnI	GAANNNNTTC	1 cut(s)	735
XspI	CTAG	9 cut(s)	126, 231, 252, 540, 830, 1181, 1587, 1818, 1826
ZraI	GACGTC	1 cut(s)	895
Zsp2I	ATGCAT	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.