Rw2G041980

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012642
Physical Location & Seq
Reverse (-)
68205851 .. 68209944
4094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041980.1

Sequence Viewer

Length: 1623 bp
ATGGATAATTCAGCTCCTGAGGTTCAACATGGAGAGGAATCAGACTCTAACAAAACCCAGATTCGTAATAAAGAGGAAAGAAATGAAGAAATTTCCTTGGTTCAACAGTCTCGAAGGCCAAACCTGTCCTCTTTGCAGATACCAGAGAGGACTCTGGAAAGTAGTTTATCTGCTTTTACGATGATTGATATTCCGTCTGTACAAAGTCCTAGTTCTATTAGAGCTGGGCTGCCGCCTAGACCACATTCAGCTAAGATCAAATCATCCGTGAAATATCTGTTTCCTTCAAAGAGCATTAGGGCGAAAAATATGACCCATGATGGCGAGAAGGCCATTCTCATTCTACCAGATACAGCTTCATCAGATGCTCTGGTGGAAAAGCCTTCTACCTCGAGGTCTTTCTCACTTAATAGGATGTTCTTCTCTCCATCATTAAAAGCAACACATTCTTTACCAGTTACACCGAGTGCAAATTCAGGTTCAGAGTCTGTGCAGGGAAGACAGTTGGAAGGTCATTCTGATTTATCTAAAATTGAAGTTGAGCAGCATATGATTCGTTCATTTTCGGTTCCAGTGAATGTTAAAACCAGAATTTTAAGGCGGATGGATTCTGGAGGCATGATGCGTGTAATTTCAACTCCACGTCCTGCAGTAGTTGATACTGCTTCCCAAAATAGTGCCCCAGCTTTGGAAACTGCTACCGAAGATCCTGGTGAGGATATTCCTGAAGAAGAAGCCGTTTGCCGAATTTGTTTGGTTGAGCTTGCAGAAGGTGGTGATACTCTTAAAATGGAGTGCAGCTGCAAAGGAGAGCTGGCACTGGCTCACAAAGAATGTGCAATAAAGTGGTTTACCATTAAAGGTAACAAGACCTGTGATGTCTGCAGACAGGATGTTCGAAACCTACATGTAACACTACTCAAAATACACAATCCTCAAACTATTATTAGACGACCACCAACAATACCTGAGCAGAGGGAAGTCGCTCGATACAGGGTCTGGCAGGACATACCAGTTCTTGTTCTGGTCAGCATGCTTGCATATTTCTGCTTTCTGGAACAACTTCTGGTCTCTGACTTGGGCCCTCGTGCTCTTGCCATTTCCTTACCTTTCTCTTGCCTTTTAGGTCTCCTTTCATCCATGATTGCTTCAACAATGGTAAGCAGGAGCTACATATGGGCCTATGCCTCCTTCCAGTTTGCCATCGTGATCCTCTTTGCTCACATATTTTATACTATCCTTAATGTAAATCCAATTCTCTCGGTCATCCTTTCCTCATTTACTGGCTTTGGGATTGCGATCAGCACAAATTCTCTTCTAGTGGAGTATCTTAGATGGAAAGCAAGCAGGCAAGTGGGATCTTCCCATCAACATACTAGCACTATGAATGAACTGCAACTGCAGCACCAGCAGTGGCAGCATGAAGAGCAATATCATCGCCATTATCAGCAACAACGACGTCAACGATTGAGAGAAGGTTCCCAGAATCAGTTACAACAGCAAGAGCAAGAGCAAGAGCAAGAGCTCCAGCATCATCAACGCCATCAGCAGCAACAACAACAATTGAATGAAGATCCAAATGCGAATGATTCCTTGCGGATAGACATTGGACGGGAGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

540

Amino Acids

61.03

Weight (kDa)

6.84

Isoelectric Point (pI)

65.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 248 - 295 1.1e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1462
AbsI CCTCGAGG 1 cut(s) 391
AciI CCGC 3 cut(s) 233, 601, 1597
AclWI GGATC 4 cut(s) 701, 1204, 1366, 1568
AcsI RAATTY 5 cut(s) 90, 472, 591, 747, 1309
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 1459
AdeI CACNNNGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 688
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 7 cut(s) 26, 104, 288, 536, 636, 1152, 1567
AjiI CACGTC 1 cut(s) 644
AjnI CCWGG 1 cut(s) 709
Alw21I GWGCWC 2 cut(s) 1093, 1527
Alw26I GTCTC 4 cut(s) 114, 1075, 1133, 1610
AlwI GGATC 4 cut(s) 701, 1204, 1366, 1568
AlwNI CAGNNNCTG 3 cut(s) 17, 488, 999
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 4 cut(s) 116, 330, 1081, 1179
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 7 cut(s) 229, 544, 798, 801, 1402, 1417, 1549
ApoI RAATTY 5 cut(s) 90, 472, 591, 747, 1309
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
Asp700I GAANNNNTTC 1 cut(s) 1062
AspS9I GGNCC 3 cut(s) 1081, 1082, 1179
AsuHPI GGTGA 2 cut(s) 725, 788
AsuII TTCGAA 1 cut(s) 898
AvaI CYCGRG 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 18
BaeGI GKGCMC 2 cut(s) 682, 1085
BanII GRGCYC 2 cut(s) 1085, 1527
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 2 cut(s) 1093, 1527
BbvI GCAGC 7 cut(s) 216, 556, 788, 810, 1414, 1429, 1561
BccI CCATC 7 cut(s) 314, 436, 598, 1211, 1329, 1374, 1551
BceAI ACGGC 1 cut(s) 722
BcgI CGANNNNNNTGC 4 cut(s) 536, 570, 1418, 1452
BciT130I CCWGG 1 cut(s) 711
BcoDI GTCTC 4 cut(s) 114, 1075, 1133, 1610
BfaI CTAG 4 cut(s) 210, 237, 1319, 1377
BfmI CTRYAG 3 cut(s) 648, 883, 1400
BisI GCNGC 8 cut(s) 230, 233, 545, 799, 802, 1403, 1418, 1550
BlsI GCNGC 8 cut(s) 231, 234, 546, 800, 803, 1404, 1419, 1551
Bme1390I CCNGG 1 cut(s) 711
BmeT110I CYCGRG 1 cut(s) 391
BmgBI CACGTC 1 cut(s) 644
BmgT120I GGNCC 3 cut(s) 1081, 1082, 1179
BmiI GGNNCC 3 cut(s) 570, 1083, 1480
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 3 cut(s) 355, 612, 1540
BpiI GAAGAC 1 cut(s) 505
BpmI CTGGAG 2 cut(s) 633, 1511
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 898
BsaBI GATNNNNATC 1 cut(s) 1298
BsaHI GRCGYC 1 cut(s) 1459
BsaI GGTCTC 2 cut(s) 1075, 1133
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse1I ACTGG 6 cut(s) 455, 572, 825, 1013, 1195, 1288
Bse21I CCTNAGG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 1298
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 6 cut(s) 263, 420, 609, 898, 1136, 1266
BseJI GATNNNNATC 1 cut(s) 1298
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 9, 960
BseNI ACTGG 6 cut(s) 455, 572, 825, 1013, 1195, 1288
BseSI GKGCMC 2 cut(s) 682, 1085
BseXI GCAGC 7 cut(s) 216, 556, 788, 810, 1414, 1429, 1561
BseYI CCCAGC 2 cut(s) 224, 682
BsgI GTGCAG 2 cut(s) 512, 817
BshFI GGCC 4 cut(s) 118, 332, 1083, 1181
BsiHKAI GWGCWC 2 cut(s) 1093, 1527
BsiHKCI CYCGRG 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 4 cut(s) 114, 1075, 1133, 1610
BsmBI CGTCTC 1 cut(s) 1610
BsnI GGCC 4 cut(s) 118, 332, 1083, 1181
Bso31I GGTCTC 2 cut(s) 1075, 1133
BsoBI CYCGRG 1 cut(s) 391
Bsp119I TTCGAA 1 cut(s) 898
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 4 cut(s) 682, 1085, 1093, 1527
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
BspACI CCGC 3 cut(s) 233, 601, 1597
BspANI GGCC 4 cut(s) 118, 332, 1083, 1181
BspCNI CTCAG 2 cut(s) 10, 961
BspLI GGNNCC 3 cut(s) 570, 1083, 1480
BspMAI CTGCAG 3 cut(s) 652, 887, 1404
BspPI GGATC 4 cut(s) 701, 1204, 1366, 1568
BspQI GCTCTTC 1 cut(s) 1419
BspT104I TTCGAA 1 cut(s) 898
BspTNI GGTCTC 2 cut(s) 1075, 1133
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 6 cut(s) 455, 572, 825, 1013, 1195, 1288
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
BssNI GRCGYC 1 cut(s) 1459
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 2 cut(s) 108, 504
Bst6I CTCTTC 2 cut(s) 1320, 1419
BstACI GRCGYC 1 cut(s) 1459
BstAUI TGTACA 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 898
BstC8I GCNNGC 6 cut(s) 765, 816, 1034, 1038, 1345, 1349
BstDEI CTNAG 4 cut(s) 18, 252, 969, 1331
BstF5I GGATG 6 cut(s) 263, 420, 609, 898, 1136, 1266
BstKTI GATC 6 cut(s) 258, 709, 1212, 1302, 1361, 1576
BstMAI GTCTC 4 cut(s) 114, 1075, 1133, 1610
BstMBI GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
BstMWI GCNNNNNNNGC 4 cut(s) 1402, 1408, 1417, 1426
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 2 cut(s) 911, 1036
BstSCI CCNGG 1 cut(s) 709
BstSFI CTRYAG 3 cut(s) 648, 883, 1400
BstSLI GKGCMC 2 cut(s) 682, 1085
BstV1I GCAGC 7 cut(s) 216, 556, 788, 810, 1414, 1429, 1561
BstV2I GAAGAC 1 cut(s) 505
BstX2I RGATCY 3 cut(s) 706, 1358, 1573
BstYI RGATCY 3 cut(s) 706, 1358, 1573
Bsu36I CCTNAGG 1 cut(s) 18
BsuRI GGCC 4 cut(s) 118, 332, 1083, 1181
BtgZI GCGATG 1 cut(s) 1421
BtrI CACGTC 1 cut(s) 644
BtsCI GGATG 6 cut(s) 263, 420, 609, 898, 1136, 1266
BtsI GCAGTG 1 cut(s) 1418
BtsIMutI CAGTG 3 cut(s) 579, 818, 1418
Cac8I GCNNGC 6 cut(s) 765, 816, 1034, 1038, 1345, 1349
CaiI CAGNNNCTG 3 cut(s) 17, 488, 999
Cfr13I GGNCC 3 cut(s) 1081, 1082, 1179
Csp6I GTAC 1 cut(s) 200
CviAII CATG 7 cut(s) 29, 317, 619, 908, 1033, 1141, 1421
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 4 cut(s) 18, 252, 969, 1331
DpnI GATC 6 cut(s) 257, 708, 1211, 1301, 1360, 1575
DpnII GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
DraIII CACNNNGTG 1 cut(s) 467
Eam1104I CTCTTC 2 cut(s) 1320, 1419
EarI CTCTTC 2 cut(s) 1320, 1419
EciI GGCGGA 1 cut(s) 616
Ecl136II GAGCTC 1 cut(s) 1525
Eco130I CCWWGG 1 cut(s) 96
Eco24I GRGCYC 2 cut(s) 1085, 1527
Eco31I GGTCTC 2 cut(s) 1075, 1133
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 1525
EcoO109I RGGNCCY 1 cut(s) 1082
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 96
EcoT38I GRGCYC 2 cut(s) 1085, 1527
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 1610
FaeI CATG 7 cut(s) 32, 320, 622, 911, 1036, 1144, 1424
FatI CATG 7 cut(s) 28, 316, 618, 907, 1032, 1140, 1420
FauNDI CATATG 2 cut(s) 549, 1175
Fnu4HI GCNGC 8 cut(s) 230, 233, 545, 799, 802, 1403, 1418, 1550
FokI GGATG 6 cut(s) 250, 427, 616, 905, 1123, 1253
FriOI GRGCYC 2 cut(s) 1085, 1527
Fsp4HI GCNGC 8 cut(s) 230, 233, 545, 799, 802, 1403, 1418, 1550
FspBI CTAG 4 cut(s) 210, 237, 1319, 1377
GluI GCNGC 8 cut(s) 230, 233, 545, 799, 802, 1403, 1418, 1550
GsaI CCCAGC 2 cut(s) 228, 686
GsuI CTGGAG 2 cut(s) 633, 1511
HaeIII GGCC 4 cut(s) 118, 332, 1083, 1181
Hin1I GRCGYC 1 cut(s) 1459
Hin1II CATG 7 cut(s) 32, 320, 622, 911, 1036, 1144, 1424
HincII GTYRAC 1 cut(s) 1463
HindII GTYRAC 1 cut(s) 1463
HinfI GANTC 9 cut(s) 38, 44, 61, 151, 485, 553, 608, 1486, 1589
HphI GGTGA 2 cut(s) 725, 788
Hpy166II GTNNAC 2 cut(s) 852, 1463
Hpy188I TCNGA 5 cut(s) 43, 364, 484, 520, 1075
Hpy188III TCNNGA 7 cut(s) 17, 111, 155, 612, 725, 1055, 1207
Hpy8I GTNNAC 2 cut(s) 852, 1463
Hpy99I CGWCG 1 cut(s) 1461
HpyAV CCTTC 8 cut(s) 108, 294, 322, 393, 503, 764, 1201, 1469
HpyCH4III ACNGT 2 cut(s) 108, 504
HpyCH4IV ACGT 3 cut(s) 643, 1459, 1619
HpyF10VI GCNNNNNNNGC 4 cut(s) 1402, 1408, 1417, 1426
HpyF3I CTNAG 4 cut(s) 18, 252, 969, 1331
HpySE526I ACGT 3 cut(s) 643, 1459, 1619
Hsp92I GRCGYC 1 cut(s) 1459
Hsp92II CATG 7 cut(s) 32, 320, 622, 911, 1036, 1144, 1424
Kzo9I GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
LguI GCTCTTC 1 cut(s) 1419
LmnI GCTCC 3 cut(s) 19, 1167, 1530
Lsp1109I GCAGC 7 cut(s) 216, 556, 788, 810, 1414, 1429, 1561
LweI GCATC 3 cut(s) 355, 612, 1540
MaeI CTAG 4 cut(s) 210, 237, 1319, 1377
MaeII ACGT 3 cut(s) 643, 1459, 1619
MaeIII GTNAC 4 cut(s) 457, 863, 910, 1491
MalI GATC 6 cut(s) 257, 708, 1211, 1301, 1360, 1575
MboI GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
MfeI CAATTG 1 cut(s) 1562
MflI RGATCY 3 cut(s) 706, 1358, 1573
MhlI GDGCHC 4 cut(s) 682, 1085, 1093, 1527
MlyI GAGTC 3 cut(s) 38, 145, 494
MmeI TCCRAC 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 7 cut(s) 408, 434, 582, 596, 786, 858, 1242
MspA1I CMGCKG 1 cut(s) 801
MspR9I CCNGG 1 cut(s) 711
MunI CAATTG 1 cut(s) 1562
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 4 cut(s) 1402, 1408, 1417, 1426
NdeI CATATG 2 cut(s) 549, 1175
NdeII GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
NlaIII CATG 7 cut(s) 32, 320, 622, 911, 1036, 1144, 1424
NlaIV GGNNCC 3 cut(s) 570, 1083, 1480
NspI RCATGY 2 cut(s) 911, 1036
NspV TTCGAA 1 cut(s) 898
PaeI GCATGC 1 cut(s) 1036
PaeR7I CTCGAG 1 cut(s) 391
PciI ACATGT 1 cut(s) 907
PciSI GCTCTTC 1 cut(s) 1419
PcsI WCGNNNNNNNCGW 1 cut(s) 1462
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 6 cut(s) 38, 61, 553, 608, 1486, 1589
PkrI GCNGC 8 cut(s) 231, 234, 546, 800, 803, 1404, 1419, 1551
PleI GAGTC 3 cut(s) 38, 145, 493
PpsI GAGTC 3 cut(s) 38, 145, 493
PscI ACATGT 1 cut(s) 907
Psp124BI GAGCTC 1 cut(s) 1527
Psp6I CCWGG 1 cut(s) 709
PspFI CCCAGC 2 cut(s) 224, 682
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 3 cut(s) 570, 1083, 1480
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 3 cut(s) 1081, 1082, 1179
PspXI VCTCGAGB 1 cut(s) 391
PstI CTGCAG 3 cut(s) 652, 887, 1404
PstNI CAGNNNCTG 3 cut(s) 17, 488, 999
PsuI RGATCY 3 cut(s) 706, 1358, 1573
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SacI GAGCTC 1 cut(s) 1527
SapI GCTCTTC 1 cut(s) 1419
SaqAI TTAA 7 cut(s) 408, 434, 582, 596, 786, 858, 1242
SatI GCNGC 8 cut(s) 230, 233, 545, 799, 802, 1403, 1418, 1550
Sau3AI GATC 6 cut(s) 255, 706, 1209, 1299, 1358, 1573
Sau96I GGNCC 3 cut(s) 1081, 1082, 1179
SchI GAGTC 3 cut(s) 38, 145, 494
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 4 cut(s) 682, 1085, 1093, 1527
SfaNI GCATC 3 cut(s) 355, 612, 1540
SfcI CTRYAG 3 cut(s) 648, 883, 1400
Sfr274I CTCGAG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 898
SlaI CTCGAG 1 cut(s) 391
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SphI GCATGC 1 cut(s) 1036
SsiI CCGC 3 cut(s) 233, 601, 1597
SspMI CTAG 4 cut(s) 210, 237, 1319, 1377
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 108, 504
TaiI ACGT 3 cut(s) 646, 1462, 1622
TaqI TCGA 4 cut(s) 112, 392, 898, 988
TaqII GACCGA 1 cut(s) 1252
TatI WGTACW 1 cut(s) 199
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 6 cut(s) 38, 61, 553, 608, 1486, 1589
Tru1I TTAA 7 cut(s) 408, 434, 582, 596, 786, 858, 1242
Tru9I TTAA 7 cut(s) 408, 434, 582, 596, 786, 858, 1242
TscAI CASTG 3 cut(s) 579, 825, 1418
TseI GCWGC 7 cut(s) 229, 544, 798, 801, 1402, 1417, 1549
TspDTI ATGAA 8 cut(s) 99, 348, 549, 1125, 1400, 1404, 1437, 1584
TspGWI ACGGA 2 cut(s) 183, 256
TspRI CASTG 3 cut(s) 579, 825, 1418
XapI RAATTY 5 cut(s) 90, 472, 591, 747, 1309
XceI RCATGY 2 cut(s) 911, 1036
XhoI CTCGAG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 4 cut(s) 210, 237, 1319, 1377
ZraI GACGTC 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.