Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0012642

Physical Location & Seq

Genomic Coordinates Forward (+)

4174604 .. 4179087

Gene Structure 4484 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g05620.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGGCGGACAATTCAGTATCTGAGCTTCAGCTTGGAGAGGAATCAACTACGGAGAGCTCATCTGATCAGCACCAGATTCATGAACAAAAGGAAAAGAATGAAGAAACCAGTTCGTCTAGACGGCCAAACCTCTCCTCGTTGCAAATACCGGCAAGGACATTGGAAACCAGTTCGTCTGCTTGTACGAGAATTGATATTCCCTCCACACCAAGTCCAAGTTCTAGTAAAGTTGGACTACCGCCTAGGCCACATTCAGCTAAGATCAAATCAACCGTGAAAAATCTGTTTCCTCAAAAAAGCTTCCGGGTGAAAAATTTGCCTCTGGATGGCGAGAAGACGATTCTCATTATGCCGGATACATCACCGTCAGATAGTCCGTTGGAAAAGCCTTCTACTTCACGGTCTTTCTCGCTCAATAAGCTTTTCTTCTCTACATCAACAAAAGGGACTCATTCTTTACCAGTTACACCAAGTGCAAATCTGGGTTCTGAGATTGGGCAGGGAAGACAAGTGGAGAGTCATTCTGATTCTAGAATTGAAGTTAAGCATCATATGTCCCGCTCTTTCTCAGTTCCAGTAAATATTGCGACCAGAAGTTTAAGGCGGATGGATTCTGGATGCATGATCCGTGTAATTTCAGCAACTCCACGTCCTTCAGCAGTTGAGCGTGCCTCACCAAATGCTGACCCAGCACTGGAAACGGCCACAGCCACTGAAGATTCGGGTGAAGATATTCCTGAAGAAGAAGCAGTTTGCAGGATTTGTTTGGTTGAGCTCGCAGAAGGTGGTGATTCTCTTAAAATGGAGTGCAGCTGCAAGGGAGAGCTAGCACTTGCTCACAAAGATTGTGCAGTAAAGTGGTTTAGCATTAAAGGGAACAAAACCTGTGACGTCTGCAAGCAGGATGTCCGAAACTTACCTGTAACACTACTCAAAATACACAATCCTCAAACTGTTATTAGACGACCACCAACAATAATGGAGCAGAGGGAAGTCCATCATTACAGGGTCTGGCAGGACATACCGGTTCTTGTCCTGGTCAGCATGCTTGCGTATTTCTGCTTTCTGGAGCAACTTCTGGTATCTGACTTGGGCCCTCGTGCTCTTGCCGTTTCCTTACCCTTCTCTTGTGTTTTAGGTATCCTTTCATCTATGATTGCTTCCACAATGGTGAGCAGGAGCTACATATGGGCTTATGCCTCCTTCCAGTTTGCCATCGTGATCCTGTTTGCCCACATATTTTATACTGTACTTAATGTAAATCCAATTCTCTCAGTCCTCCTTTCCTCATTCACTGGCTTCGGGATTGCAATCAGCACGAATTCCCTTCTGGTTGAGTATCGTAGATGGAAAATCAGCAGACAACTGCAATCATCCTATCAACATGCTAGCAGTGCACTGCATCTGCATGAACTACAACTGCAGCAGCACCGGCAACAGCACCAGCAGTGGCTACAGCAAGAAGAGCAATATCAACGCCAATCCCAGCAACAACGACAGCAACAGTTGATGGAAGATTCACATGATCAGTACCAGCAAGTACAGCGACAGCTTCAGCAGCAAGAAGAGGAGCATCATCGACACCAACAGGAGCACCAGCACCAACGGTTGGATGGAGATCCAAATGCTGATGGTTCTAGGCCGCTAGAAGTTAGACAGGGCACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RINGv

Protein Analysis

556

Amino Acids

62.44

Weight (kDa)

6.77

Isoelectric Point (pI)

72.9

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012642)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g40120
malus_domestica	MD09G1135100.v1.1 MD17G1124100.v1.1
prunus_persica	Prupe.3G191600_v2.0.a1 Prupe.3G191600_v2.0.a1 Prupe.3G191600_v2.0.a1
pyrus_communis	pycom09g05620 pycom17g11550
rosa_chinensis	RchiOBHm_Chr2g0154751
rosa_laevigata	RLG00000020800
rosa_multiflora	Rmu_sc0001004.1_g000002 Rmu_sc0001027.1_g000003
rosa_roxburghii	Rroxscaffold_2G00094130
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2AG510400 Rh2BG521300 Rh2CG495600 Rh2DG531400
rosa_wichuraiana	Rw2G041980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	894
AccB7I	CCANNNNNTGG	1 cut(s)	1609
AccBSI	CCGCTC	1 cut(s)	561
AciI	CCGC	5 cut(s)	5, 239, 559, 604, 1643
AclWI	GGATC	3 cut(s)	619, 1216, 1613
AcoI	YGGCCR	2 cut(s)	122, 702
AcsI	RAATTY	2 cut(s)	313, 1321
AcuI	CTGAAG	5 cut(s)	11, 639, 735, 759, 1538
AcyI	GRCGYC	1 cut(s)	891
AdeI	CACNNNGTG	1 cut(s)	473
AfaI	GTAC	4 cut(s)	184, 1251, 1532, 1542
AfiI	CCNNNNNNNGG	3 cut(s)	326, 694, 1609
AgeI	ACCGGT	1 cut(s)	1024
AgsI	TTSAA	1 cut(s)	539
AjiI	CACGTC	1 cut(s)	650
AjnI	CCWGG	1 cut(s)	1035
AjuI	GAANNNNNNNTTGG	2 cut(s)	202, 234
AleI	CACNNNNGTG	1 cut(s)	1169
Alw21I	GWGCWC	5 cut(s)	59, 777, 1105, 1399, 1596
Alw44I	GTGCAC	1 cut(s)	1395
AlwI	GGATC	3 cut(s)	619, 1216, 1613
AlwNI	CAGNNNCTG	3 cut(s)	20, 713, 1011
AoxI	GGCC	5 cut(s)	122, 245, 702, 1093, 1640
ApaI	GGGCCC	1 cut(s)	1097
ApaLI	GTGCAC	1 cut(s)	1395
ApeKI	GCWGC	5 cut(s)	810, 813, 1423, 1426, 1558
ApoI	RAATTY	2 cut(s)	313, 1321
AsiGI	ACCGGT	1 cut(s)	1024
Asp700I	GAANNNNTTC	1 cut(s)	732
AspA2I	CCTAGG	1 cut(s)	242
AspS9I	GGNCC	2 cut(s)	1093, 1094
AsuC2I	CCSGG	1 cut(s)	305
AsuHPI	GGTGA	6 cut(s)	319, 354, 666, 737, 800, 1183
AsuNHI	GCTAGC	2 cut(s)	826, 1388
AvrII	CCTAGG	1 cut(s)	242
BaeGI	GKGCMC	3 cut(s)	1097, 1399, 1664
BanII	GRGCYC	3 cut(s)	59, 777, 1097
BarI	GAAGNNNNNNTAC	2 cut(s)	9, 41
BauI	CACGAG	1 cut(s)	1098
BbsI	GAAGAC	2 cut(s)	341, 511
Bbv12I	GWGCWC	5 cut(s)	59, 777, 1105, 1399, 1596
BbvI	GCAGC	5 cut(s)	800, 822, 1435, 1438, 1570
BccI	CCATC	8 cut(s)	320, 601, 1005, 1223, 1341, 1504, 1607, 1625
BceAI	ACGGC	3 cut(s)	137, 717, 1094
BciT130I	CCWGG	1 cut(s)	1037
BciVI	GTATCC	2 cut(s)	349, 1151
BclI	TGATCA	2 cut(s)	64, 1525
BcnI	CCSGG	1 cut(s)	305
BfaI	CTAG	8 cut(s)	117, 222, 243, 531, 827, 1389, 1638, 1646
BfmI	CTRYAG	2 cut(s)	1421, 1454
BfuI	GTATCC	2 cut(s)	349, 1151
BisI	GCNGC	6 cut(s)	811, 814, 1424, 1427, 1559, 1643
BlnI	CCTAGG	1 cut(s)	242
BlsI	GCNGC	6 cut(s)	812, 815, 1425, 1428, 1560, 1644
Bme1390I	CCNGG	2 cut(s)	305, 1037
BmgBI	CACGTC	1 cut(s)	650
BmgT120I	GGNCC	2 cut(s)	1093, 1094
BmiI	GGNNCC	1 cut(s)	1095
BmrFI	CCNGG	2 cut(s)	305, 1037
BmsI	GCATC	4 cut(s)	556, 608, 1411, 1582
BmtI	GCTAGC	2 cut(s)	830, 1392
BpiI	GAAGAC	2 cut(s)	341, 511
BplI	GAGNNNNNCTC	2 cut(s)	656, 688
BpmI	CTGGAG	1 cut(s)	1088
BpuMI	CCSGG	1 cut(s)	305
BsaBI	GATNNNNATC	2 cut(s)	1310, 1617
BsaHI	GRCGYC	1 cut(s)	891
BsaJI	CCNNGG	1 cut(s)	242
BsaWI	WCCGGW	1 cut(s)	1024
Bsc4I	CCNNNNNNNGG	3 cut(s)	326, 694, 1609
Bse118I	RCCGGY	3 cut(s)	148, 1024, 1431
Bse1I	ACTGG	7 cut(s)	108, 168, 461, 575, 699, 1207, 1300
Bse8I	GATNNNNATC	2 cut(s)	1310, 1617
BseBI	CCWGG	1 cut(s)	1037
BseDI	CCNNGG	1 cut(s)	242
BseGI	GGATG	6 cut(s)	331, 612, 623, 910, 1373, 1618
BseJI	GATNNNNATC	2 cut(s)	1310, 1617
BseLI	CCNNNNNNNGG	3 cut(s)	326, 694, 1609
BseMII	CTCAG	4 cut(s)	12, 480, 582, 1287
BseNI	ACTGG	7 cut(s)	108, 168, 461, 575, 699, 1207, 1300
BseRI	GAGGAG	2 cut(s)	124, 1583
BseSI	GKGCMC	3 cut(s)	1097, 1399, 1664
BseXI	GCAGC	5 cut(s)	800, 822, 1435, 1438, 1570
BseYI	CCCAGC	2 cut(s)	688, 1485
BsgI	GTGCAG	2 cut(s)	829, 870
BshFI	GGCC	5 cut(s)	124, 247, 704, 1095, 1642
BshTI	ACCGGT	1 cut(s)	1024
BsiHKAI	GWGCWC	5 cut(s)	59, 777, 1105, 1399, 1596
BsiSI	CCGG	5 cut(s)	149, 304, 353, 1025, 1432
BslFI	GGGAC	2 cut(s)	460, 541
BslI	CCNNNNNNNGG	3 cut(s)	326, 694, 1609
BsmFI	GGGAC	2 cut(s)	460, 541
BsnI	GGCC	5 cut(s)	124, 247, 704, 1095, 1642
Bsp120I	GGGCCC	1 cut(s)	1093
Bsp1286I	GDGCHC	7 cut(s)	59, 777, 1097, 1105, 1399, 1596, 1664
Bsp143I	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
BspACI	CCGC	5 cut(s)	5, 239, 559, 604, 1643
BspANI	GGCC	5 cut(s)	124, 247, 704, 1095, 1642
BspCNI	CTCAG	4 cut(s)	13, 481, 581, 1286
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	1 cut(s)	1095
BspMAI	CTGCAG	1 cut(s)	1425
BspOI	GCTAGC	2 cut(s)	830, 1392
BspPI	GGATC	3 cut(s)	619, 1216, 1613
BspQI	GCTCTTC	1 cut(s)	1458
BsrBI	CCGCTC	1 cut(s)	561
BsrFI	RCCGGY	3 cut(s)	148, 1024, 1431
BsrI	ACTGG	7 cut(s)	108, 168, 461, 575, 699, 1207, 1300
BssAI	RCCGGY	3 cut(s)	148, 1024, 1431
BssECI	CCNNGG	1 cut(s)	242
BssMI	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
BssNI	GRCGYC	1 cut(s)	891
BssSI	CACGAG	1 cut(s)	1098
BssT1I	CCWWGG	1 cut(s)	242
Bst2BI	CACGAG	1 cut(s)	1098
Bst2UI	CCWGG	1 cut(s)	1037
Bst4CI	ACNGT	7 cut(s)	274, 366, 402, 955, 1249, 1506, 1608
Bst6I	CTCTTC	2 cut(s)	1458, 1560
BstACI	GRCGYC	1 cut(s)	891
BstC8I	GCNNGC	7 cut(s)	669, 777, 828, 899, 1046, 1050, 1390
BstDEI	CTNAG	5 cut(s)	21, 258, 489, 568, 1273
BstF5I	GGATG	6 cut(s)	331, 612, 623, 910, 1373, 1618
BstKTI	GATC	6 cut(s)	67, 264, 627, 1224, 1528, 1621
BstMBI	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
BstMWI	GCNNNNNNNGC	7 cut(s)	418, 689, 1394, 1432, 1465, 1543, 1558
BstNI	CCWGG	1 cut(s)	1037
BstNSI	RCATGY	2 cut(s)	1048, 1388
BstSCI	CCNGG	2 cut(s)	303, 1035
BstSFI	CTRYAG	2 cut(s)	1421, 1454
BstSLI	GKGCMC	3 cut(s)	1097, 1399, 1664
BstV1I	GCAGC	5 cut(s)	800, 822, 1435, 1438, 1570
BstV2I	GAAGAC	2 cut(s)	341, 511
BstX2I	RGATCY	1 cut(s)	1618
BstYI	RGATCY	1 cut(s)	1618
BsuI	GTATCC	2 cut(s)	349, 1151
BsuRI	GGCC	5 cut(s)	124, 247, 704, 1095, 1642
BtrI	CACGTC	1 cut(s)	650
BtsCI	GGATG	6 cut(s)	331, 612, 623, 910, 1373, 1618
BtsI	GCAGTG	3 cut(s)	1397, 1399, 1454
BtsIMutI	CAGTG	6 cut(s)	692, 711, 1293, 1397, 1399, 1454
Cac8I	GCNNGC	7 cut(s)	669, 777, 828, 899, 1046, 1050, 1390
CaiI	CAGNNNCTG	3 cut(s)	20, 713, 1011
CciI	TCATGA	1 cut(s)	79
Cfr10I	RCCGGY	3 cut(s)	148, 1024, 1431
Cfr13I	GGNCC	2 cut(s)	1093, 1094
Csp6I	GTAC	4 cut(s)	183, 1250, 1531, 1541
CspAI	ACCGGT	1 cut(s)	1024
CviAII	CATG	6 cut(s)	80, 622, 1045, 1385, 1409, 1523
CviQI	GTAC	4 cut(s)	183, 1250, 1531, 1541
DdeI	CTNAG	5 cut(s)	21, 258, 489, 568, 1273
DpnI	GATC	6 cut(s)	66, 263, 626, 1223, 1527, 1620
DpnII	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
DraIII	CACNNNGTG	1 cut(s)	473
EaeI	YGGCCR	2 cut(s)	122, 702
Eam1104I	CTCTTC	2 cut(s)	1458, 1560
EarI	CTCTTC	2 cut(s)	1458, 1560
EciI	GGCGGA	2 cut(s)	20, 619
Ecl136II	GAGCTC	2 cut(s)	57, 775
Eco130I	CCWWGG	1 cut(s)	242
Eco24I	GRGCYC	3 cut(s)	59, 777, 1097
Eco53kI	GAGCTC	2 cut(s)	57, 775
Eco57I	CTGAAG	5 cut(s)	11, 639, 735, 759, 1538
EcoICRI	GAGCTC	2 cut(s)	57, 775
EcoO109I	RGGNCCY	1 cut(s)	1094
EcoRI	GAATTC	1 cut(s)	1321
EcoRII	CCWGG	1 cut(s)	1035
EcoT14I	CCWWGG	1 cut(s)	242
EcoT22I	ATGCAT	1 cut(s)	623
EcoT38I	GRGCYC	3 cut(s)	59, 777, 1097
ErhI	CCWWGG	1 cut(s)	242
FaeI	CATG	6 cut(s)	83, 625, 1048, 1388, 1412, 1526
FalI	AAGNNNNNCTT	2 cut(s)	410, 442
FaqI	GGGAC	2 cut(s)	460, 541
FatI	CATG	6 cut(s)	79, 621, 1044, 1384, 1408, 1522
FauI	CCCGC	1 cut(s)	566
FauNDI	CATATG	2 cut(s)	552, 1187
FbaI	TGATCA	2 cut(s)	64, 1525
Fnu4HI	GCNGC	6 cut(s)	811, 814, 1424, 1427, 1559, 1643
FokI	GGATG	6 cut(s)	338, 619, 630, 917, 1360, 1625
FriOI	GRGCYC	3 cut(s)	59, 777, 1097
Fsp4HI	GCNGC	6 cut(s)	811, 814, 1424, 1427, 1559, 1643
FspBI	CTAG	8 cut(s)	117, 222, 243, 531, 827, 1389, 1638, 1646
GluI	GCNGC	6 cut(s)	811, 814, 1424, 1427, 1559, 1643
GsaI	CCCAGC	2 cut(s)	692, 1489
GsuI	CTGGAG	1 cut(s)	1088
HaeIII	GGCC	5 cut(s)	124, 247, 704, 1095, 1642
HapII	CCGG	5 cut(s)	149, 304, 353, 1025, 1432
Hin1I	GRCGYC	1 cut(s)	891
Hin1II	CATG	6 cut(s)	83, 625, 1048, 1388, 1412, 1526
HindIII	AAGCTT	2 cut(s)	298, 419
HpaII	CCGG	5 cut(s)	149, 304, 353, 1025, 1432
HphI	GGTGA	6 cut(s)	319, 354, 666, 737, 800, 1183
Hpy166II	GTNNAC	1 cut(s)	1397
Hpy188I	TCNGA	7 cut(s)	22, 64, 370, 490, 526, 911, 1087
Hpy188III	TCNNGA	9 cut(s)	80, 117, 323, 531, 615, 737, 1067, 1219, 1303
Hpy8I	GTNNAC	1 cut(s)	1397
HpyAV	CCTTC	6 cut(s)	399, 663, 776, 1132, 1213, 1337
HpyCH4III	ACNGT	7 cut(s)	274, 366, 402, 955, 1249, 1506, 1608
HpyCH4IV	ACGT	2 cut(s)	649, 891
HpyF10VI	GCNNNNNNNGC	7 cut(s)	418, 689, 1394, 1432, 1465, 1543, 1558
HpyF3I	CTNAG	5 cut(s)	21, 258, 489, 568, 1273
HpySE526I	ACGT	2 cut(s)	649, 891
Hsp92I	GRCGYC	1 cut(s)	891
Hsp92II	CATG	6 cut(s)	83, 625, 1048, 1388, 1412, 1526
Ksp22I	TGATCA	2 cut(s)	64, 1525
Kzo9I	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
LguI	GCTCTTC	1 cut(s)	1458
LmnI	GCTCC	5 cut(s)	982, 1069, 1179, 1570, 1591
Lsp1109I	GCAGC	5 cut(s)	800, 822, 1435, 1438, 1570
LweI	GCATC	4 cut(s)	556, 608, 1411, 1582
MaeI	CTAG	8 cut(s)	117, 222, 243, 531, 827, 1389, 1638, 1646
MaeII	ACGT	2 cut(s)	649, 891
MaeIII	GTNAC	3 cut(s)	463, 887, 922
MalI	GATC	6 cut(s)	66, 263, 626, 1223, 1527, 1620
MbiI	CCGCTC	1 cut(s)	561
MboI	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
MflI	RGATCY	1 cut(s)	1618
MhlI	GDGCHC	7 cut(s)	59, 777, 1097, 1105, 1399, 1596, 1664
MluCI	AATT	7 cut(s)	10, 189, 313, 534, 633, 1266, 1321
MlyI	GAGTC	2 cut(s)	442, 526
MmeI	TCCRAC	3 cut(s)	211, 360, 1590
Mph1103I	ATGCAT	1 cut(s)	623
MroXI	GAANNNNTTC	1 cut(s)	732
MseI	TTAA	5 cut(s)	543, 599, 798, 870, 1254
MslI	CAYNNNNRTG	2 cut(s)	1169, 1407
MspA1I	CMGCKG	1 cut(s)	813
MspI	CCGG	5 cut(s)	149, 304, 353, 1025, 1432
MspR9I	CCNGG	2 cut(s)	305, 1037
MvaI	CCWGG	1 cut(s)	1037
MwoI	GCNNNNNNNGC	7 cut(s)	418, 689, 1394, 1432, 1465, 1543, 1558
NciI	CCSGG	1 cut(s)	305
NdeI	CATATG	2 cut(s)	552, 1187
NdeII	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
NheI	GCTAGC	2 cut(s)	826, 1388
NlaIII	CATG	6 cut(s)	83, 625, 1048, 1388, 1412, 1526
NlaIV	GGNNCC	1 cut(s)	1095
NmuCI	GTSAC	1 cut(s)	887
NsiI	ATGCAT	1 cut(s)	623
NspI	RCATGY	2 cut(s)	1048, 1388
OliI	CACNNNNGTG	1 cut(s)	1169
PaeI	GCATGC	1 cut(s)	1048
PagI	TCATGA	1 cut(s)	79
PciSI	GCTCTTC	1 cut(s)	1458
PdmI	GAANNNNTTC	1 cut(s)	732
PfeI	GAWTC	8 cut(s)	41, 76, 340, 527, 611, 719, 791, 1517
PflMI	CCANNNNNTGG	1 cut(s)	1609
PinAI	ACCGGT	1 cut(s)	1024
PkrI	GCNGC	6 cut(s)	812, 815, 1425, 1428, 1560, 1644
PleI	GAGTC	2 cut(s)	442, 525
PpsI	GAGTC	2 cut(s)	442, 525
Psp124BI	GAGCTC	2 cut(s)	59, 777
Psp6I	CCWGG	1 cut(s)	1035
PspFI	CCCAGC	2 cut(s)	688, 1485
PspGI	CCWGG	1 cut(s)	1035
PspN4I	GGNNCC	1 cut(s)	1095
PspOMI	GGGCCC	1 cut(s)	1093
PspPI	GGNCC	2 cut(s)	1093, 1094
PstI	CTGCAG	1 cut(s)	1425
PstNI	CAGNNNCTG	3 cut(s)	20, 713, 1011
PsuI	RGATCY	1 cut(s)	1618
PvuII	CAGCTG	1 cut(s)	813
RsaI	GTAC	4 cut(s)	184, 1251, 1532, 1542
RsaNI	GTAC	4 cut(s)	183, 1250, 1531, 1541
RseI	CAYNNNNRTG	2 cut(s)	1169, 1407
SacI	GAGCTC	2 cut(s)	59, 777
SapI	GCTCTTC	1 cut(s)	1458
SaqAI	TTAA	5 cut(s)	543, 599, 798, 870, 1254
SatI	GCNGC	6 cut(s)	811, 814, 1424, 1427, 1559, 1643
Sau3AI	GATC	6 cut(s)	64, 261, 624, 1221, 1525, 1618
Sau96I	GGNCC	2 cut(s)	1093, 1094
SchI	GAGTC	2 cut(s)	442, 526
ScrFI	CCNGG	2 cut(s)	305, 1037
SduI	GDGCHC	7 cut(s)	59, 777, 1097, 1105, 1399, 1596, 1664
SfaNI	GCATC	4 cut(s)	556, 608, 1411, 1582
SfcI	CTRYAG	2 cut(s)	1421, 1454
SmiMI	CAYNNNNRTG	2 cut(s)	1169, 1407
SphI	GCATGC	1 cut(s)	1048
Sse9I	AATT	7 cut(s)	10, 189, 313, 534, 633, 1266, 1321
SsiI	CCGC	5 cut(s)	5, 239, 559, 604, 1643
SspI	AATATT	1 cut(s)	583
SspMI	CTAG	8 cut(s)	117, 222, 243, 531, 827, 1389, 1638, 1646
SstI	GAGCTC	2 cut(s)	59, 777
StyD4I	CCNGG	2 cut(s)	303, 1035
StyI	CCWWGG	1 cut(s)	242
TaaI	ACNGT	7 cut(s)	274, 366, 402, 955, 1249, 1506, 1608
TaiI	ACGT	2 cut(s)	652, 894
TaqI	TCGA	1 cut(s)	1579
TasI	AATT	7 cut(s)	10, 189, 313, 534, 633, 1266, 1321
TatI	WGTACW	2 cut(s)	1249, 1540
TauI	GCSGC	1 cut(s)	1645
TfiI	GAWTC	8 cut(s)	41, 76, 340, 527, 611, 719, 791, 1517
Tru1I	TTAA	5 cut(s)	543, 599, 798, 870, 1254
Tru9I	TTAA	5 cut(s)	543, 599, 798, 870, 1254
TscAI	CASTG	6 cut(s)	699, 718, 1300, 1399, 1404, 1454
TseFI	GTSAC	1 cut(s)	887
TseI	GCWGC	5 cut(s)	810, 813, 1423, 1426, 1558
Tsp45I	GTSAC	1 cut(s)	887
TspDTI	ATGAA	5 cut(s)	68, 96, 114, 1137, 1425
TspGWI	ACGGA	3 cut(s)	65, 366, 617
TspRI	CASTG	6 cut(s)	699, 718, 1300, 1399, 1404, 1454
Van91I	CCANNNNNTGG	1 cut(s)	1609
VneI	GTGCAC	1 cut(s)	1395
XapI	RAATTY	2 cut(s)	313, 1321
XbaI	TCTAGA	2 cut(s)	116, 530
XceI	RCATGY	2 cut(s)	1048, 1388
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1610
XmaJI	CCTAGG	1 cut(s)	242
XmnI	GAANNNNTTC	1 cut(s)	732
XspI	CTAG	8 cut(s)	117, 222, 243, 531, 827, 1389, 1638, 1646
ZraI	GACGTC	1 cut(s)	892
Zsp2I	ATGCAT	1 cut(s)	623

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.