Rh2BG521300

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012642
Physical Location & Seq
Reverse (-)
73188463 .. 73193733
5271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG521300.1

Sequence Viewer

Length: 1221 bp
ATGGAGAATTCAGCTCCTGAGGTTCAACATGGAGAGGAATCAGACTCTAACAAAACCCAGATTCGTAATCAAGAGGAAAGAAATGAAGAAATTTCCTTGGTTCAACAGTCTCGAAGGCCAAACCTGTCTTCTTTGCAGATACCAGAGAGGACTCTGGAAAGTAGTTTGTCTGCTTTTACGATGATTGATATTCCATCTGTACAAAGTCCTAGTTCTATTAGAGCTGGGCTGCCGCCTAGACCACATTCAGCTAAGATCAAATCATCCGTAAAATATCTGTTTCCTTCAAAGAGCATTAGGGCGAAAAATATGACCCATGATGGCGAGAAGGCCATTCTCATTCTACCAGATACAGCTTCATCAGATGCTCTGGTGGAAAAGCCTTCTACCTCGAGGTCTTTCTCACTTAATAGGATGTTCTTCTCTCCATCATTAAAAGCAACACATTCTTTACCAGTTACACCGAGTGCAAATTCAGGTTCAGAGTCTGTGCAGGGAAGACAGTTGGAAGGTCATTCTGATTTATCTAAAATTGAAGTTGAGCAGCATATGATTCGCTCATTTTCGGTTCCAGTGAATGTTAAAACCAGAATTTTAAGGCGGATGGATTCTGGAGGCATGATGCGTGTAATTTCAACTCCACGTCCTGCAGTAGTTGATACTGCTTCCCAAAATAGTGCCCCAGCTATGGAAACTGCTACCGAAGATCCTGGTGAGGATATTCCTGAAGAAGAAGCCGTTTGCCGAATTTGTTTGGTTGAGCTTGCAGAAGGTGGTGATACTCTTAAAATGGAGTGCAGCTGCAAAGGAGAGCTGGCACTGGCTCACAAAGAATGTGCAATAAAGTGGTTTACCATTAAAGGTAACAAGACCTGTGATGTCTGCAGACAGGATGTTCGAAACCTACATGTAACACTACTCAAAATACACAATCCTCAAACTATTATTAGACGACCACCAACAATACCTGAGCAGAGGGAAGTCGCTCGATACAGGGTCTGGCAGGACATACCAGTTCTTGTTCTGGTCAGCATGCTTGCATATTTCTGCTTTCTGGAACAACTTCTGGTCTCTGACTTGGGCCCTCGTGCTCTTGCCATTTCCTTACCTTTCTCTTGCCTTTTAGGTCTCCTTTCATCCATGATTGCTTCAACAATGGGTGAGCTATTCTTTTTGTTATATATCAAGTTTTTTTTGTGTTACTACAAATTCGTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

406

Amino Acids

45.19

Weight (kDa)

7.1

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 248 - 295 7.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 391
AciI CCGC 2 cut(s) 233, 601
AclWI GGATC 1 cut(s) 701
AcsI RAATTY 6 cut(s) 7, 90, 472, 591, 747, 1208
AcuI CTGAAG 1 cut(s) 747
AdeI CACNNNGTG 1 cut(s) 467
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 688
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 6 cut(s) 26, 104, 288, 536, 636, 1152
AjiI CACGTC 1 cut(s) 644
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 2 cut(s) 112, 144
AluBI AGCT 9 cut(s) 14, 224, 251, 356, 686, 763, 801, 814, 1165
AluI AGCT 9 cut(s) 14, 224, 251, 356, 686, 763, 801, 814, 1165
Alw21I GWGCWC 1 cut(s) 1093
Alw26I GTCTC 3 cut(s) 114, 1075, 1133
AlwI GGATC 1 cut(s) 701
AlwNI CAGNNNCTG 3 cut(s) 17, 488, 999
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 3 cut(s) 116, 330, 1081
ApaI GGGCCC 1 cut(s) 1085
ApeKI GCWGC 4 cut(s) 229, 544, 798, 801
ApoI RAATTY 6 cut(s) 7, 90, 472, 591, 747, 1208
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
Asp700I GAANNNNTTC 1 cut(s) 1062
AspS9I GGNCC 2 cut(s) 1081, 1082
AsuHPI GGTGA 3 cut(s) 725, 788, 1172
AsuII TTCGAA 1 cut(s) 898
AvaI CYCGRG 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 18
BaeGI GKGCMC 2 cut(s) 682, 1085
BanII GRGCYC 1 cut(s) 1085
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 2 cut(s) 120, 505
Bbv12I GWGCWC 1 cut(s) 1093
BbvI GCAGC 4 cut(s) 216, 556, 788, 810
BccI CCATC 4 cut(s) 202, 314, 436, 598
BceAI ACGGC 1 cut(s) 722
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BciT130I CCWGG 1 cut(s) 711
BcoDI GTCTC 3 cut(s) 114, 1075, 1133
BfaI CTAG 2 cut(s) 210, 237
BfmI CTRYAG 2 cut(s) 648, 883
BisI GCNGC 5 cut(s) 230, 233, 545, 799, 802
BlsI GCNGC 5 cut(s) 231, 234, 546, 800, 803
Bme1390I CCNGG 1 cut(s) 711
BmeT110I CYCGRG 1 cut(s) 391
BmgBI CACGTC 1 cut(s) 644
BmgT120I GGNCC 2 cut(s) 1081, 1082
BmiI GGNNCC 2 cut(s) 570, 1083
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 2 cut(s) 355, 612
BpiI GAAGAC 2 cut(s) 120, 505
BpmI CTGGAG 1 cut(s) 633
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 898
BsaI GGTCTC 2 cut(s) 1075, 1133
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse1I ACTGG 4 cut(s) 455, 572, 825, 1013
Bse21I CCTNAGG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 5 cut(s) 263, 420, 609, 898, 1136
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 9, 960
BseNI ACTGG 4 cut(s) 455, 572, 825, 1013
BseSI GKGCMC 2 cut(s) 682, 1085
BseXI GCAGC 4 cut(s) 216, 556, 788, 810
BseYI CCCAGC 2 cut(s) 224, 682
BsgI GTGCAG 2 cut(s) 512, 817
BshFI GGCC 3 cut(s) 118, 332, 1083
BsiHKAI GWGCWC 1 cut(s) 1093
BsiHKCI CYCGRG 1 cut(s) 391
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 3 cut(s) 114, 1075, 1133
BsnI GGCC 3 cut(s) 118, 332, 1083
Bso31I GGTCTC 2 cut(s) 1075, 1133
BsoBI CYCGRG 1 cut(s) 391
Bsp119I TTCGAA 1 cut(s) 898
Bsp120I GGGCCC 1 cut(s) 1081
Bsp1286I GDGCHC 3 cut(s) 682, 1085, 1093
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 2 cut(s) 255, 706
BspACI CCGC 2 cut(s) 233, 601
BspANI GGCC 3 cut(s) 118, 332, 1083
BspCNI CTCAG 2 cut(s) 10, 961
BspLI GGNNCC 2 cut(s) 570, 1083
BspMAI CTGCAG 2 cut(s) 652, 887
BspPI GGATC 1 cut(s) 701
BspT104I TTCGAA 1 cut(s) 898
BspTNI GGTCTC 2 cut(s) 1075, 1133
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 4 cut(s) 455, 572, 825, 1013
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 255, 706
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 2 cut(s) 108, 504
BstAUI TGTACA 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 898
BstC8I GCNNGC 4 cut(s) 765, 816, 1034, 1038
BstDEI CTNAG 3 cut(s) 18, 252, 969
BstF5I GGATG 5 cut(s) 263, 420, 609, 898, 1136
BstKTI GATC 2 cut(s) 258, 709
BstMAI GTCTC 3 cut(s) 114, 1075, 1133
BstMBI GATC 2 cut(s) 255, 706
BstNI CCWGG 1 cut(s) 711
BstNSI RCATGY 2 cut(s) 911, 1036
BstSCI CCNGG 1 cut(s) 709
BstSFI CTRYAG 2 cut(s) 648, 883
BstSLI GKGCMC 2 cut(s) 682, 1085
BstV1I GCAGC 4 cut(s) 216, 556, 788, 810
BstV2I GAAGAC 2 cut(s) 120, 505
BstX2I RGATCY 1 cut(s) 706
BstYI RGATCY 1 cut(s) 706
Bsu36I CCTNAGG 1 cut(s) 18
BsuRI GGCC 3 cut(s) 118, 332, 1083
BtrI CACGTC 1 cut(s) 644
BtsCI GGATG 5 cut(s) 263, 420, 609, 898, 1136
BtsIMutI CAGTG 2 cut(s) 579, 818
Cac8I GCNNGC 4 cut(s) 765, 816, 1034, 1038
CaiI CAGNNNCTG 3 cut(s) 17, 488, 999
Cfr13I GGNCC 2 cut(s) 1081, 1082
Csp6I GTAC 1 cut(s) 200
CviAII CATG 6 cut(s) 29, 317, 619, 908, 1033, 1141
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 18, 252, 969
DpnI GATC 2 cut(s) 257, 708
DpnII GATC 2 cut(s) 255, 706
DraIII CACNNNGTG 1 cut(s) 467
EciI GGCGGA 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 1085
Eco31I GGTCTC 2 cut(s) 1075, 1133
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 18
Eco88I CYCGRG 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 1082
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 1085
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 6 cut(s) 32, 320, 622, 911, 1036, 1144
FatI CATG 6 cut(s) 28, 316, 618, 907, 1032, 1140
FauNDI CATATG 1 cut(s) 549
Fnu4HI GCNGC 5 cut(s) 230, 233, 545, 799, 802
FokI GGATG 5 cut(s) 250, 427, 616, 905, 1123
FriOI GRGCYC 1 cut(s) 1085
Fsp4HI GCNGC 5 cut(s) 230, 233, 545, 799, 802
FspBI CTAG 2 cut(s) 210, 237
GluI GCNGC 5 cut(s) 230, 233, 545, 799, 802
GsaI CCCAGC 2 cut(s) 228, 686
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 118, 332, 1083
Hin1II CATG 6 cut(s) 32, 320, 622, 911, 1036, 1144
HinfI GANTC 7 cut(s) 38, 44, 61, 151, 485, 553, 608
HphI GGTGA 3 cut(s) 725, 788, 1172
Hpy166II GTNNAC 1 cut(s) 852
Hpy188I TCNGA 5 cut(s) 43, 364, 484, 520, 1075
Hpy188III TCNNGA 7 cut(s) 17, 71, 111, 155, 612, 725, 1055
Hpy8I GTNNAC 1 cut(s) 852
HpyAV CCTTC 6 cut(s) 108, 294, 322, 393, 503, 764
HpyCH4III ACNGT 2 cut(s) 108, 504
HpyCH4IV ACGT 1 cut(s) 643
HpyF3I CTNAG 3 cut(s) 18, 252, 969
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 6 cut(s) 32, 320, 622, 911, 1036, 1144
Kzo9I GATC 2 cut(s) 255, 706
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 4 cut(s) 216, 556, 788, 810
LweI GCATC 2 cut(s) 355, 612
MaeI CTAG 2 cut(s) 210, 237
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 4 cut(s) 457, 863, 910, 1199
MalI GATC 2 cut(s) 257, 708
MboI GATC 2 cut(s) 255, 706
MboII GAAGA 7 cut(s) 98, 120, 412, 510, 716, 740, 743
MflI RGATCY 1 cut(s) 706
MhlI GDGCHC 3 cut(s) 682, 1085, 1093
MluCI AATT 8 cut(s) 7, 90, 472, 531, 591, 630, 747, 1208
MlyI GAGTC 3 cut(s) 38, 145, 494
MmeI TCCRAC 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 6 cut(s) 408, 434, 582, 596, 786, 858
MspA1I CMGCKG 1 cut(s) 801
MspR9I CCNGG 1 cut(s) 711
MvaI CCWGG 1 cut(s) 711
NdeI CATATG 1 cut(s) 549
NdeII GATC 2 cut(s) 255, 706
NlaIII CATG 6 cut(s) 32, 320, 622, 911, 1036, 1144
NlaIV GGNNCC 2 cut(s) 570, 1083
NspI RCATGY 2 cut(s) 911, 1036
NspV TTCGAA 1 cut(s) 898
PaeI GCATGC 1 cut(s) 1036
PaeR7I CTCGAG 1 cut(s) 391
PciI ACATGT 1 cut(s) 907
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 4 cut(s) 38, 61, 553, 608
PkrI GCNGC 5 cut(s) 231, 234, 546, 800, 803
PleI GAGTC 3 cut(s) 38, 145, 493
PpsI GAGTC 3 cut(s) 38, 145, 493
PscI ACATGT 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 709
PspFI CCCAGC 2 cut(s) 224, 682
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 2 cut(s) 570, 1083
PspOMI GGGCCC 1 cut(s) 1081
PspPI GGNCC 2 cut(s) 1081, 1082
PspXI VCTCGAGB 1 cut(s) 391
PstI CTGCAG 2 cut(s) 652, 887
PstNI CAGNNNCTG 3 cut(s) 17, 488, 999
PsuI RGATCY 1 cut(s) 706
PvuII CAGCTG 1 cut(s) 801
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SaqAI TTAA 6 cut(s) 408, 434, 582, 596, 786, 858
SatI GCNGC 5 cut(s) 230, 233, 545, 799, 802
Sau3AI GATC 2 cut(s) 255, 706
Sau96I GGNCC 2 cut(s) 1081, 1082
SchI GAGTC 3 cut(s) 38, 145, 494
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 3 cut(s) 682, 1085, 1093
SfaNI GCATC 2 cut(s) 355, 612
SfcI CTRYAG 2 cut(s) 648, 883
Sfr274I CTCGAG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 898
SlaI CTCGAG 1 cut(s) 391
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SphI GCATGC 1 cut(s) 1036
Sse9I AATT 8 cut(s) 7, 90, 472, 531, 591, 630, 747, 1208
SsiI CCGC 2 cut(s) 233, 601
SspMI CTAG 2 cut(s) 210, 237
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 2 cut(s) 108, 504
TaiI ACGT 1 cut(s) 646
TaqI TCGA 4 cut(s) 112, 392, 898, 988
TasI AATT 8 cut(s) 7, 90, 472, 531, 591, 630, 747, 1208
TatI WGTACW 1 cut(s) 199
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 4 cut(s) 38, 61, 553, 608
Tru1I TTAA 6 cut(s) 408, 434, 582, 596, 786, 858
Tru9I TTAA 6 cut(s) 408, 434, 582, 596, 786, 858
TscAI CASTG 2 cut(s) 579, 825
TseI GCWGC 4 cut(s) 229, 544, 798, 801
TspDTI ATGAA 4 cut(s) 99, 348, 1125, 1206
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 2 cut(s) 579, 825
XapI RAATTY 6 cut(s) 7, 90, 472, 591, 747, 1208
XceI RCATGY 2 cut(s) 911, 1036
XhoI CTCGAG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 2 cut(s) 210, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.