MD17G1124100.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012642
Physical Location & Seq
Forward (+)
10779791 .. 10785177
5387 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1124100.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGACGGAGAATTCAGCTTCCGAGCTTCGGCAGGGTGAGGAATTGACTTTGGAGAGCTCCTCTGAGCAGTCCCAGATTCATGATCAAAAGTTAAAGAATGAAGAAACCTTGTTGGTTCAGCAGTCTAGACGACCAAACCTTTCCTCATTGCAAATACCGGCAAGGACACTGGAAAGTAGTTTGTCCACTTTTACGAGAATTGATATTCCCTTTGCGCCAAGTCCAAGTTCTAGTAGAGTTGGACTGCCGCCTAGGCCACATTCAGCTAAGATCAAATCAACGGTGAAAAATCTGTTTCCTCAAAAAAGCTTTAGGGTGAAAAATTTGCCCCTGGATGGTGAGAAGACCATTCTCATTGTACCGGACACCCCCCCATCAGATAGTCCTTTGGAAAAGCCTTCTACTTCGCGTTCTTTCTCTCTTAATAAGGTTTTCTTGTCTACATCAATGAAAGGGACACATTCTTTACCAGTTACACCAAGTGCAAATCTGGGTTCTGAGACTGGGCAGGGAAGACATATGGAGAGTCATTCTGATTATCCTAGAATTGAAGCTAAGCATCATATGTCCCGCTCATTCTCAGTTCCAGTAAATATAACAGGCAGAAGTTTAAGGCGGATGGATTCTGGAGGCATGATCCGTGTAATTTCAGCAACTCCACGTCCTTCAGCAGTTGAGGGTGCCTCACCAAATGCTGACCCAGCACTGGAAACCGCTACTGAAGATTCTGGTGAGGATATTCCCGAAGAAGAAGCAGTTTGCAGGATTTGTTTGGTTGAGCTCGCAGAAGGTGGTGATTCTCTTAAAATGGAGTGCAGCTGCAAAGGAGAGCTGGCGCTTGCTCACAAAGACTGTGCAGTAAAGTGGTTTAGCATTAAAGGGAACAAGACCTGTGATGTCTGCAAGCAGGATGTCCGAAACTTACCTGTAACACTACTCAAAATACACAATCCTCAAACTGTTACTAGACGACCACCAACAATAATGGAGCATAGGGAAGTTCCTCGTTACAGGGTCTGGCAGGATATACCAATTCTTGTCCTGGTCAGCATGCTTGCGTATTTCTGCTTTCTGGAGCAACTTCTGGTATCTGACTTGGGCCCTCGTGCTCTTGCCATTTCTTTACCCTTCTCTTGTGTTTTAGGTCTCCTTTCATCCGTGATTGCTTCCACAATGGTGAGCAGGAGCTACATATGGGCTTATGCCTCCTTCCAGTTTGCCATTGTGATCCTGTTTGCTCACATATTTTATACTGTTCTTAATGTAAATCCAATTCTCTCGGTTCTACTTTCCTCATTCACCGGGTTTGGGATTGCAATCAGCACAAATTCCCTTCTGGTGGAGTATCTTAGATGGAAAACCAGCAGACAACTGCAATCTTCCCATCAACACACTAGCAATGCATTGCATCGGCATGAACTACAACTGCAGCAGCACCGGCAACAGCAATGGCAGCAGCAAGAAGAGCAATATCAACGCCAATACCAGCAACGACGGCAACAATTGATGGAAGATTCACATAATCAGTACCAGCAACTACAACGACAGCTGCAGCAGCAAGAAGAAGAGCATCGTCGACACCAACAGGAGCAACAGCTGGAACAGTTTGACGGAGATCTAAATACTGATGGTTCCAGGCAGCTAGAAGTTGGACAGGGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

555

Amino Acids

62.49

Weight (kDa)

7.04

Isoelectric Point (pI)

70.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 254 - 301 7.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 573
AccI GTMKAC 2 cut(s) 440, 1576
AccII CGCG 1 cut(s) 409
AciI CCGC 4 cut(s) 248, 571, 616, 714
AclWI GGATC 2 cut(s) 631, 1222
AcsI RAATTY 3 cut(s) 10, 322, 1327
AcuI CTGAAG 2 cut(s) 651, 741
AdeI CACNNNGTG 1 cut(s) 482
AfaI GTAC 2 cut(s) 360, 1529
AfiI CCNNNNNNNGG 5 cut(s) 27, 335, 706, 1308, 1339
AgsI TTSAA 1 cut(s) 551
AjiI CACGTC 1 cut(s) 662
AjnI CCWGG 3 cut(s) 330, 1041, 1634
AjuI GAANNNNNNNTTGG 2 cut(s) 211, 243
AleI CACNNNNGTG 1 cut(s) 1175
Alw21I GWGCWC 3 cut(s) 59, 783, 1111
Alw26I GTCTC 2 cut(s) 494, 1151
AlwI GGATC 2 cut(s) 631, 1222
AlwNI CAGNNNCTG 1 cut(s) 1017
AoxI GGCC 2 cut(s) 254, 1099
ApaI GGGCCC 1 cut(s) 1103
ApoI RAATTY 3 cut(s) 10, 322, 1327
AspA2I CCTAGG 1 cut(s) 251
AspLEI GCGC 2 cut(s) 217, 838
AspS9I GGNCC 2 cut(s) 1099, 1100
AsuC2I CCSGG 1 cut(s) 1303
AsuHPI GGTGA 9 cut(s) 47, 295, 328, 350, 678, 743, 806, 1189, 1291
AvrII CCTAGG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 1103
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 3 cut(s) 59, 783, 1103
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 2 cut(s) 350, 520
Bbv12I GWGCWC 3 cut(s) 59, 783, 1111
BccI CCATC 7 cut(s) 329, 382, 613, 1347, 1392, 1501, 1622
BceAI ACGGC 1 cut(s) 1511
BciT130I CCWGG 3 cut(s) 332, 1043, 1636
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 1 cut(s) 1303
BcoDI GTCTC 2 cut(s) 494, 1151
BfaI CTAG 7 cut(s) 126, 231, 252, 543, 966, 1395, 1643
BfmI CTRYAG 2 cut(s) 1427, 1550
BfoI RGCGCY 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 253
BglII AGATCT 1 cut(s) 1615
BlnI CCTAGG 1 cut(s) 251
BlpI GCTNAGC 1 cut(s) 555
Bme1390I CCNGG 4 cut(s) 332, 1043, 1303, 1636
BmgBI CACGTC 1 cut(s) 662
BmgT120I GGNCC 2 cut(s) 1099, 1100
BmiI GGNNCC 3 cut(s) 682, 1101, 1633
BmrFI CCNGG 4 cut(s) 332, 1043, 1303, 1636
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 3 cut(s) 568, 1417, 1579
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 2 cut(s) 350, 520
BplI GAGNNNNNCTC 4 cut(s) 44, 76, 668, 700
BpmI CTGGAG 2 cut(s) 648, 1094
Bpu1102I GCTNAGC 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 1303
BsaBI GATNNNNATC 1 cut(s) 1316
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 2 cut(s) 251, 330
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 335, 706, 1308, 1339
Bse118I RCCGGY 2 cut(s) 157, 1437
Bse1I ACTGG 6 cut(s) 174, 470, 508, 587, 711, 1213
Bse3DI GCAATG 4 cut(s) 146, 1403, 1405, 1454
Bse8I GATNNNNATC 1 cut(s) 1316
BseBI CCWGG 3 cut(s) 332, 1043, 1636
BseDI CCNNGG 2 cut(s) 251, 330
BseGI GGATG 4 cut(s) 340, 624, 916, 1154
BseJI GATNNNNATC 1 cut(s) 1316
BseLI CCNNNNNNNGG 5 cut(s) 27, 335, 706, 1308, 1339
BseMI GCAATG 4 cut(s) 146, 1403, 1405, 1454
BseMII CTCAG 3 cut(s) 54, 489, 594
BseNI ACTGG 6 cut(s) 174, 470, 508, 587, 711, 1213
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 1103
BseYI CCCAGC 1 cut(s) 700
BsgI GTGCAG 2 cut(s) 835, 876
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 2 cut(s) 256, 1101
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 3 cut(s) 59, 783, 1111
BsiSI CCGG 4 cut(s) 158, 362, 1302, 1438
BslFI GGGAC 3 cut(s) 55, 469, 553
BslI CCNNNNNNNGG 5 cut(s) 27, 335, 706, 1308, 1339
BsmAI GTCTC 2 cut(s) 494, 1151
BsmFI GGGAC 3 cut(s) 55, 469, 553
BsnI GGCC 2 cut(s) 256, 1101
Bso31I GGTCTC 1 cut(s) 1151
Bsp120I GGGCCC 1 cut(s) 1099
Bsp1286I GDGCHC 4 cut(s) 59, 783, 1103, 1111
Bsp143I GATC 5 cut(s) 82, 270, 636, 1227, 1615
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 4 cut(s) 248, 571, 616, 714
BspANI GGCC 2 cut(s) 256, 1101
BspCNI CTCAG 3 cut(s) 55, 490, 593
BspFNI CGCG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 682, 1101, 1633
BspMAI CTGCAG 2 cut(s) 1431, 1554
BspPI GGATC 2 cut(s) 631, 1222
BspQI GCTCTTC 2 cut(s) 1458, 1560
BspT107I GGYRCC 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 1151
BsrBI CCGCTC 1 cut(s) 573
BsrDI GCAATG 4 cut(s) 146, 1403, 1405, 1454
BsrFI RCCGGY 2 cut(s) 157, 1437
BsrI ACTGG 6 cut(s) 174, 470, 508, 587, 711, 1213
BssAI RCCGGY 2 cut(s) 157, 1437
BssECI CCNNGG 2 cut(s) 251, 330
BssMI GATC 5 cut(s) 82, 270, 636, 1227, 1615
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 3 cut(s) 332, 1043, 1636
Bst4CI ACNGT 5 cut(s) 283, 854, 961, 1255, 1605
Bst6I CTCTTC 2 cut(s) 1458, 1560
BstC8I GCNNGC 6 cut(s) 783, 834, 840, 905, 1052, 1056
BstDEI CTNAG 6 cut(s) 63, 267, 498, 555, 580, 1349
BstF5I GGATG 4 cut(s) 340, 624, 916, 1154
BstFNI CGCG 1 cut(s) 409
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 2 cut(s) 217, 838
BstKTI GATC 5 cut(s) 85, 273, 639, 1230, 1618
BstMAI GTCTC 2 cut(s) 494, 1151
BstMBI GATC 5 cut(s) 82, 270, 636, 1227, 1615
BstMWI GCNNNNNNNGC 7 cut(s) 253, 701, 1438, 1453, 1465, 1495, 1555
BstNI CCWGG 3 cut(s) 332, 1043, 1636
BstNSI RCATGY 1 cut(s) 1054
BstSCI CCNGG 4 cut(s) 330, 1041, 1301, 1634
BstSFI CTRYAG 2 cut(s) 1427, 1550
BstSLI GKGCMC 1 cut(s) 1103
BstUI CGCG 1 cut(s) 409
BstV2I GAAGAC 2 cut(s) 350, 520
BstX2I RGATCY 1 cut(s) 1615
BstYI RGATCY 1 cut(s) 1615
BsuRI GGCC 2 cut(s) 256, 1101
BtrI CACGTC 1 cut(s) 662
BtsCI GGATG 4 cut(s) 340, 624, 916, 1154
BtsIMutI CAGTG 2 cut(s) 167, 704
Cac8I GCNNGC 6 cut(s) 783, 834, 840, 905, 1052, 1056
CaiI CAGNNNCTG 1 cut(s) 1017
CciI TCATGA 1 cut(s) 79
CfoI GCGC 2 cut(s) 217, 838
Cfr10I RCCGGY 2 cut(s) 157, 1437
Cfr13I GGNCC 2 cut(s) 1099, 1100
Csp6I GTAC 2 cut(s) 359, 1528
CviAII CATG 4 cut(s) 80, 634, 1051, 1415
CviQI GTAC 2 cut(s) 359, 1528
DdeI CTNAG 6 cut(s) 63, 267, 498, 555, 580, 1349
DpnI GATC 5 cut(s) 84, 272, 638, 1229, 1617
DpnII GATC 5 cut(s) 82, 270, 636, 1227, 1615
DraIII CACNNNGTG 1 cut(s) 482
Eam1104I CTCTTC 2 cut(s) 1458, 1560
EarI CTCTTC 2 cut(s) 1458, 1560
EciI GGCGGA 1 cut(s) 631
Ecl136II GAGCTC 2 cut(s) 57, 781
Eco130I CCWWGG 1 cut(s) 251
Eco24I GRGCYC 3 cut(s) 59, 783, 1103
Eco31I GGTCTC 1 cut(s) 1151
Eco53kI GAGCTC 2 cut(s) 57, 781
Eco57I CTGAAG 2 cut(s) 651, 741
EcoICRI GAGCTC 2 cut(s) 57, 781
EcoO109I RGGNCCY 1 cut(s) 1100
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 3 cut(s) 330, 1041, 1634
EcoT14I CCWWGG 1 cut(s) 251
EcoT22I ATGCAT 1 cut(s) 1405
EcoT38I GRGCYC 3 cut(s) 59, 783, 1103
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 4 cut(s) 83, 637, 1054, 1418
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 3 cut(s) 55, 469, 553
FatI CATG 4 cut(s) 79, 633, 1050, 1414
FauI CCCGC 1 cut(s) 578
FauNDI CATATG 3 cut(s) 519, 564, 1193
FbaI TGATCA 1 cut(s) 82
FblI GTMKAC 2 cut(s) 440, 1576
FokI GGATG 4 cut(s) 347, 631, 923, 1141
FriOI GRGCYC 3 cut(s) 59, 783, 1103
FspBI CTAG 7 cut(s) 126, 231, 252, 543, 966, 1395, 1643
GlaI GCGC 2 cut(s) 216, 837
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 2 cut(s) 648, 1094
HaeII RGCGCY 1 cut(s) 839
HaeIII GGCC 2 cut(s) 256, 1101
HapII CCGG 4 cut(s) 158, 362, 1302, 1438
HhaI GCGC 2 cut(s) 217, 838
Hin1II CATG 4 cut(s) 83, 637, 1054, 1418
Hin6I GCGC 2 cut(s) 215, 836
HinP1I GCGC 2 cut(s) 215, 836
HincII GTYRAC 1 cut(s) 1577
HindII GTYRAC 1 cut(s) 1577
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 6 cut(s) 76, 526, 623, 725, 797, 1514
HpaII CCGG 4 cut(s) 158, 362, 1302, 1438
HphI GGTGA 9 cut(s) 47, 295, 328, 350, 678, 743, 806, 1189, 1291
Hpy166II GTNNAC 3 cut(s) 186, 441, 1577
Hpy188I TCNGA 7 cut(s) 22, 64, 379, 499, 535, 917, 1093
Hpy188III TCNNGA 5 cut(s) 80, 126, 627, 743, 1073
Hpy8I GTNNAC 3 cut(s) 186, 441, 1577
Hpy99I CGWCG 2 cut(s) 1497, 1578
HpyAV CCTTC 6 cut(s) 408, 675, 782, 1138, 1219, 1343
HpyCH4III ACNGT 5 cut(s) 283, 854, 961, 1255, 1605
HpyCH4IV ACGT 1 cut(s) 661
HpyF10VI GCNNNNNNNGC 7 cut(s) 253, 701, 1438, 1453, 1465, 1495, 1555
HpyF3I CTNAG 6 cut(s) 63, 267, 498, 555, 580, 1349
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 4 cut(s) 83, 637, 1054, 1418
HspAI GCGC 2 cut(s) 215, 836
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 5 cut(s) 82, 270, 636, 1227, 1615
LguI GCTCTTC 2 cut(s) 1458, 1560
LmnI GCTCC 5 cut(s) 62, 988, 1075, 1185, 1588
LweI GCATC 3 cut(s) 568, 1417, 1579
MaeI CTAG 7 cut(s) 126, 231, 252, 543, 966, 1395, 1643
MaeII ACGT 1 cut(s) 661
MaeIII GTNAC 4 cut(s) 472, 928, 961, 1007
MalI GATC 5 cut(s) 84, 272, 638, 1229, 1617
MbiI CCGCTC 1 cut(s) 573
MboI GATC 5 cut(s) 82, 270, 636, 1227, 1615
MfeI CAATTG 1 cut(s) 1502
MflI RGATCY 1 cut(s) 1615
MhlI GDGCHC 4 cut(s) 59, 783, 1103, 1111
MlyI GAGTC 1 cut(s) 535
MmeI TCCRAC 2 cut(s) 220, 1630
Mph1103I ATGCAT 1 cut(s) 1405
MseI TTAA 6 cut(s) 92, 423, 611, 804, 876, 1260
MslI CAYNNNNRTG 2 cut(s) 1175, 1413
MspA1I CMGCKG 3 cut(s) 819, 1549, 1597
MspI CCGG 4 cut(s) 158, 362, 1302, 1438
MspR9I CCNGG 4 cut(s) 332, 1043, 1303, 1636
MunI CAATTG 1 cut(s) 1502
MvaI CCWGG 3 cut(s) 332, 1043, 1636
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 7 cut(s) 253, 701, 1438, 1453, 1465, 1495, 1555
NciI CCSGG 1 cut(s) 1303
NdeI CATATG 3 cut(s) 519, 564, 1193
NdeII GATC 5 cut(s) 82, 270, 636, 1227, 1615
NlaIII CATG 4 cut(s) 83, 637, 1054, 1418
NlaIV GGNNCC 3 cut(s) 682, 1101, 1633
NsiI ATGCAT 1 cut(s) 1405
NspI RCATGY 1 cut(s) 1054
OliI CACNNNNGTG 1 cut(s) 1175
PaeI GCATGC 1 cut(s) 1054
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 1458, 1560
PfeI GAWTC 5 cut(s) 76, 623, 725, 797, 1514
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
Psp124BI GAGCTC 2 cut(s) 59, 783
Psp6I CCWGG 3 cut(s) 330, 1041, 1634
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 3 cut(s) 330, 1041, 1634
PspN4I GGNNCC 3 cut(s) 682, 1101, 1633
PspOMI GGGCCC 1 cut(s) 1099
PspPI GGNCC 2 cut(s) 1099, 1100
PstI CTGCAG 2 cut(s) 1431, 1554
PstNI CAGNNNCTG 1 cut(s) 1017
PsuI RGATCY 1 cut(s) 1615
PvuII CAGCTG 3 cut(s) 819, 1549, 1597
RsaI GTAC 2 cut(s) 360, 1529
RsaNI GTAC 2 cut(s) 359, 1528
RseI CAYNNNNRTG 2 cut(s) 1175, 1413
SacI GAGCTC 2 cut(s) 59, 783
SalI GTCGAC 1 cut(s) 1575
SapI GCTCTTC 2 cut(s) 1458, 1560
SaqAI TTAA 6 cut(s) 92, 423, 611, 804, 876, 1260
Sau3AI GATC 5 cut(s) 82, 270, 636, 1227, 1615
Sau96I GGNCC 2 cut(s) 1099, 1100
SchI GAGTC 1 cut(s) 535
ScrFI CCNGG 4 cut(s) 332, 1043, 1303, 1636
SduI GDGCHC 4 cut(s) 59, 783, 1103, 1111
SfaNI GCATC 3 cut(s) 568, 1417, 1579
SfcI CTRYAG 2 cut(s) 1427, 1550
SmiMI CAYNNNNRTG 2 cut(s) 1175, 1413
SphI GCATGC 1 cut(s) 1054
SsiI CCGC 4 cut(s) 248, 571, 616, 714
SspMI CTAG 7 cut(s) 126, 231, 252, 543, 966, 1395, 1643
SstI GAGCTC 2 cut(s) 59, 783
StyD4I CCNGG 4 cut(s) 330, 1041, 1301, 1634
StyI CCWWGG 1 cut(s) 251
TaaI ACNGT 5 cut(s) 283, 854, 961, 1255, 1605
TaiI ACGT 1 cut(s) 664
TaqI TCGA 1 cut(s) 1576
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 5 cut(s) 76, 623, 725, 797, 1514
Tru1I TTAA 6 cut(s) 92, 423, 611, 804, 876, 1260
Tru9I TTAA 6 cut(s) 92, 423, 611, 804, 876, 1260
TscAI CASTG 2 cut(s) 174, 711
TspDTI ATGAA 5 cut(s) 68, 114, 464, 1143, 1431
TspGWI ACGGA 4 cut(s) 20, 629, 1147, 1626
TspRI CASTG 2 cut(s) 174, 711
XapI RAATTY 3 cut(s) 10, 322, 1327
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 1 cut(s) 1054
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 440, 1576
XspI CTAG 7 cut(s) 126, 231, 252, 543, 966, 1395, 1643
Zsp2I ATGCAT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.