FvH4_6g40440

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016899
Physical Location & Seq
Reverse (-)
31966171 .. 31966977
807 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40440.t1

Sequence Viewer

Length: 807 bp
ATGTGCAAATGTTATATAGGCTTAGTGGTTCTGCTCCAGAGTAGTAATGCTAGATGTCCAAGTACTTTGCAGAAGAACTATAGCCTAGTTGCCATGCTCTACAAGTTCCTCAATGCCCATAAACTCCAGCCGTTCTCAAAGCGTGTGCCGGTGCCGCCTAAGCAAGCTCCGGCACAGTCAGAGGAGAATGGTAACTCTGTTTTGAAGGTTGTTCACGCAGGTGGAAATGTTGAGAGTTACTACATGGCTATTCCTGCAGCCAGGATTTTGGAGAAGTACCCTTCTTTCTTGCTTGCTAGGCCTGAAGTCTTTAGGCGGCCATGGGACTCGGTAGTGAAGCCGGATGCAATCCTCACACCCGGAGAGAAAGTTTTTCTTGTTCCCCGCCGGACTGTGAAGAAGCTCCGTCGCAAAATACGGAGACCCGGGAAGGATTTCTCAGCCAATTCCTTCATATCTCAATCGTATAAGGATGTGTCAACTGAGACCCTTTCACAGAGGAGACATGTTGGTAGTGAAGTTGGTAATGCGAGTGGGAGTGACGCTTCCATTTGTAGTGCTATTAGCAGAAAGAAAGGTGATGGCAAGAAGCATGTGACTTTTTTTGGCATCAAGAAACATGTGACATTTGCTGGCCTTGATGTGAAGCACAAGCCAGAGAAGACTCCTAGTGAAACTGAGCAAGATTCGAGTAAAAGCTCAAGTTCTCTAAAATCTGTTGGAGGGAGAAGCAGGGTTCGGATTGTGACATGGGAGCCGACTCTTGCAGCAATAACAGAAACTCATGGGAATGATTTGATGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

269

Amino Acids

29.68

Weight (kDa)

10.06

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 59 - 260 2e-15 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 151
AciI CCGC 3 cut(s) 155, 316, 385
AcoI YGGCCR 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 2 cut(s) 64, 278
AflIII ACRYGT 2 cut(s) 505, 619
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 260
AleI CACNNNNGTG 1 cut(s) 219
AluBI AGCT 3 cut(s) 167, 403, 699
AluI AGCT 3 cut(s) 167, 403, 699
Alw26I GTCTC 3 cut(s) 415, 479, 496
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 3 cut(s) 299, 317, 634
ApeKI GCWGC 2 cut(s) 257, 767
Asp700I GAANNNNTTC 1 cut(s) 434
AsuC2I CCSGG 3 cut(s) 360, 426, 427
AsuHPI GGTGA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 425
BanI GGYRCC 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 668
BbvI GCAGC 2 cut(s) 269, 779
BccI CCATC 1 cut(s) 575
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 3 cut(s) 360, 426, 427
BcoDI GTCTC 3 cut(s) 415, 479, 496
BfaI CTAG 4 cut(s) 51, 86, 297, 669
BfmI CTRYAG 2 cut(s) 79, 255
BfuAI ACCTGC 1 cut(s) 209
BisI GCNGC 4 cut(s) 155, 258, 317, 768
BlsI GCNGC 4 cut(s) 156, 259, 318, 769
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 4 cut(s) 262, 360, 426, 427
BmeT110I CYCGRG 1 cut(s) 425
BmiI GGNNCC 2 cut(s) 153, 756
BmrFI CCNGG 4 cut(s) 262, 360, 426, 427
BmsI GCATC 2 cut(s) 334, 618
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 2 cut(s) 20, 110
Bpu10I CCTNAGC 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 685
BpuMI CCSGG 3 cut(s) 360, 426, 427
BsaI GGTCTC 2 cut(s) 415, 479
BsaJI CCNNGG 2 cut(s) 320, 425
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bse118I RCCGGY 1 cut(s) 148
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 2 cut(s) 320, 425
BseGI GGATG 2 cut(s) 349, 478
BseMII CTCAG 3 cut(s) 453, 474, 669
BseRI GAGGAG 2 cut(s) 197, 514
BseXI GCAGC 2 cut(s) 269, 779
BshFI GGCC 3 cut(s) 301, 319, 636
BshNI GGYRCC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 6 cut(s) 149, 170, 341, 360, 388, 426
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 3 cut(s) 415, 479, 496
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 3 cut(s) 301, 319, 636
Bso31I GGTCTC 2 cut(s) 415, 479
BsoBI CYCGRG 1 cut(s) 425
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 3 cut(s) 155, 316, 385
BspANI GGCC 3 cut(s) 301, 319, 636
BspCNI CTCAG 3 cut(s) 452, 475, 670
BspLI GGNNCC 2 cut(s) 153, 756
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 2 cut(s) 415, 479
BsrFI RCCGGY 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 320, 425
BssT1I CCWWGG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 2 cut(s) 177, 394
BstC8I GCNNGC 3 cut(s) 165, 294, 634
BstDEI CTNAG 5 cut(s) 22, 159, 439, 483, 678
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 349, 478
BstMAI GTCTC 3 cut(s) 415, 479, 496
BstMWI GCNNNNNNNGC 4 cut(s) 154, 160, 254, 298
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 3 cut(s) 509, 596, 623
BstSCI CCNGG 4 cut(s) 260, 358, 424, 425
BstSFI CTRYAG 2 cut(s) 79, 255
BstV1I GCAGC 2 cut(s) 269, 779
BstV2I GAAGAC 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 268
BsuRI GGCC 3 cut(s) 301, 319, 636
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 349, 478
BveI ACCTGC 1 cut(s) 209
Cac8I GCNNGC 3 cut(s) 165, 294, 634
Cfr10I RCCGGY 1 cut(s) 148
Cfr9I CCCGGG 1 cut(s) 425
CseI GACGC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 63, 277
CviAII CATG 8 cut(s) 94, 244, 321, 506, 593, 620, 750, 785
CviQI GTAC 2 cut(s) 63, 277
DdeI CTNAG 5 cut(s) 22, 159, 439, 483, 678
EaeI YGGCCR 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 320
Eco147I AGGCCT 1 cut(s) 301
Eco31I GGTCTC 2 cut(s) 415, 479
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 320
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 8 cut(s) 97, 247, 324, 509, 596, 623, 753, 788
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 1 cut(s) 338
FatI CATG 8 cut(s) 93, 243, 320, 505, 592, 619, 749, 784
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 4 cut(s) 155, 258, 317, 768
FokI GGATG 2 cut(s) 356, 485
Fsp4HI GCNGC 4 cut(s) 155, 258, 317, 768
FspBI CTAG 4 cut(s) 51, 86, 297, 669
GluI GCNGC 4 cut(s) 155, 258, 317, 768
GsuI CTGGAG 2 cut(s) 20, 110
HaeIII GGCC 3 cut(s) 301, 319, 636
HapII CCGG 6 cut(s) 149, 170, 341, 360, 388, 426
HgaI GACGC 1 cut(s) 551
Hin1II CATG 8 cut(s) 97, 247, 324, 509, 596, 623, 753, 788
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 4 cut(s) 326, 664, 686, 760
HpaII CCGG 6 cut(s) 149, 170, 341, 360, 388, 426
HphI GGTGA 1 cut(s) 590
Hpy166II GTNNAC 2 cut(s) 214, 480
Hpy188I TCNGA 2 cut(s) 181, 741
Hpy188III TCNNGA 2 cut(s) 37, 613
Hpy8I GTNNAC 2 cut(s) 214, 480
Hpy99I CGWCG 1 cut(s) 411
HpyAV CCTTC 4 cut(s) 199, 291, 424, 460
HpyCH4III ACNGT 2 cut(s) 177, 394
HpyCH4V TGCA 5 cut(s) 6, 70, 257, 347, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 160, 254, 298
HpyF3I CTNAG 5 cut(s) 22, 159, 439, 483, 678
Hsp92II CATG 8 cut(s) 97, 247, 324, 509, 596, 623, 753, 788
LmnI GCTCC 4 cut(s) 39, 172, 408, 754
Lsp1109I GCAGC 2 cut(s) 269, 779
LweI GCATC 2 cut(s) 334, 618
MaeI CTAG 4 cut(s) 51, 86, 297, 669
MaeIII GTNAC 6 cut(s) 191, 236, 539, 595, 622, 745
MboII GAAGA 3 cut(s) 85, 409, 673
MluCI AATT 1 cut(s) 445
MlyI GAGTC 3 cut(s) 320, 658, 754
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 5 cut(s) 119, 175, 362, 492, 716
MroXI GAANNNNTTC 1 cut(s) 434
MslI CAYNNNNRTG 2 cut(s) 219, 789
MspI CCGG 6 cut(s) 149, 170, 341, 360, 388, 426
MspR9I CCNGG 4 cut(s) 262, 360, 426, 427
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 4 cut(s) 154, 160, 254, 298
NciI CCSGG 3 cut(s) 360, 426, 427
NcoI CCATGG 1 cut(s) 320
NlaIII CATG 8 cut(s) 97, 247, 324, 509, 596, 623, 753, 788
NlaIV GGNNCC 2 cut(s) 153, 756
NmuCI GTSAC 4 cut(s) 539, 595, 622, 745
NspI RCATGY 3 cut(s) 509, 596, 623
OliI CACNNNNGTG 1 cut(s) 219
PaqCI CACCTGC 1 cut(s) 209
PceI AGGCCT 1 cut(s) 301
PciI ACATGT 2 cut(s) 505, 619
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 686
PkrI GCNGC 4 cut(s) 156, 259, 318, 769
PleI GAGTC 3 cut(s) 320, 658, 754
PpsI GAGTC 3 cut(s) 320, 658, 754
PscI ACATGT 2 cut(s) 505, 619
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 153, 756
PstI CTGCAG 1 cut(s) 259
RsaI GTAC 2 cut(s) 64, 278
RsaNI GTAC 2 cut(s) 63, 277
RseI CAYNNNNRTG 2 cut(s) 219, 789
SatI GCNGC 4 cut(s) 155, 258, 317, 768
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 3 cut(s) 320, 658, 754
ScrFI CCNGG 4 cut(s) 262, 360, 426, 427
SetI ASST 6 cut(s) 169, 210, 223, 405, 580, 701
SfaNI GCATC 2 cut(s) 334, 618
SfcI CTRYAG 2 cut(s) 79, 255
SmaI CCCGGG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 219, 789
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
Sse9I AATT 1 cut(s) 445
SseBI AGGCCT 1 cut(s) 301
SsiI CCGC 3 cut(s) 155, 316, 385
SspMI CTAG 4 cut(s) 51, 86, 297, 669
StuI AGGCCT 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 260, 358, 424, 425
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 2 cut(s) 177, 394
TaqI TCGA 1 cut(s) 689
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 62
TauI GCSGC 2 cut(s) 157, 319
TfiI GAWTC 1 cut(s) 686
TseFI GTSAC 4 cut(s) 539, 595, 622, 745
TseI GCWGC 2 cut(s) 257, 767
Tsp45I GTSAC 4 cut(s) 539, 595, 622, 745
TspDTI ATGAA 1 cut(s) 442
TspGWI ACGGA 2 cut(s) 395, 433
TspMI CCCGGG 1 cut(s) 425
XceI RCATGY 3 cut(s) 509, 596, 623
XmaI CCCGGG 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 434
XspI CTAG 4 cut(s) 51, 86, 297, 669
ZrmI AGTACT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.