pycom09g05300

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0016899
Physical Location & Seq
Forward (+)
3947875 .. 3948174
300 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g05300.1

Sequence Viewer

Length: 300 bp
ATGTTCCATAAGTTTCTCAACGACCGTAAACTCAGACCGTTCTCACGGCGGATGCAACCCCCAGAAGCAGAGCCGGAGGTGGAGGAGGACGGTACTTCTACCTTGAAGATCGTCCATATCGGTGGAGGTGTTGAGAAGTACTACATGGCTACTCCTGCGGCGAAGATACTGGAGAAGCACCCCTCTTTCTTGCTGGCTAGGCCTGAGGTTTTTAGGCGGCCCTGGGACTCGGTAGTTAAACCTAATGATCGATCCTCACGCCCGGTGAGAAAGTACTCCTTATTCCTCACCGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

100

Amino Acids

11.57

Weight (kDa)

9.75

Isoelectric Point (pI)

78.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 31 - 72 1.7e-06 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 49, 158, 217
AclWI GGATC 1 cut(s) 246
AfaI GTAC 3 cut(s) 94, 140, 275
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 1 cut(s) 295
AluI AGCT 1 cut(s) 295
AlwI GGATC 1 cut(s) 246
AoxI GGCC 2 cut(s) 200, 218
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 277, 280
AxyI CCTNAGG 1 cut(s) 204
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 223
BcnI CCSGG 1 cut(s) 263
BfaI CTAG 1 cut(s) 198
BisI GCNGC 2 cut(s) 159, 218
BlsI GCNGC 2 cut(s) 160, 219
BmcAI AGTACT 2 cut(s) 140, 275
Bme1390I CCNGG 2 cut(s) 223, 263
BmgT120I GGNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 223, 263
BmsI GCATC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 263
Bsa29I ATCGAT 1 cut(s) 250
BsaJI CCNNGG 2 cut(s) 221, 222
Bse1I ACTGG 1 cut(s) 174
Bse21I CCTNAGG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 223
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 2 cut(s) 221, 222
BseGI GGATG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 46, 195
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 2 cut(s) 202, 220
BshVI ATCGAT 1 cut(s) 250
BsiEI CGRYCG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 74, 263
BslFI GGGAC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 2 cut(s) 202, 220
Bsp143I GATC 3 cut(s) 108, 247, 251
BspACI CCGC 3 cut(s) 49, 158, 217
BspANI GGCC 2 cut(s) 202, 220
BspCNI CTCAG 2 cut(s) 45, 196
BspDI ATCGAT 1 cut(s) 250
BspPI GGATC 1 cut(s) 246
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 2 cut(s) 221, 222
BssMI GATC 3 cut(s) 108, 247, 251
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 3 cut(s) 26, 39, 92
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 32, 204
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 3 cut(s) 111, 250, 254
BstMBI GATC 3 cut(s) 108, 247, 251
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 2 cut(s) 155, 199
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 221, 261
BstXI CCANNNNNNTGG 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 250
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 2 cut(s) 202, 220
BsuTUI ATCGAT 1 cut(s) 250
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 219
ClaI ATCGAT 1 cut(s) 250
Csp6I GTAC 3 cut(s) 93, 139, 274
CviAII CATG 1 cut(s) 145
CviJI RGCY 6 cut(s) 73, 149, 197, 202, 220, 295
CviKI_1 RGCY 6 cut(s) 73, 149, 197, 202, 220, 295
CviQI GTAC 3 cut(s) 93, 139, 274
DdeI CTNAG 2 cut(s) 32, 204
DpnI GATC 3 cut(s) 110, 249, 253
DpnII GATC 3 cut(s) 108, 247, 251
EciI GGCGGA 1 cut(s) 64
Eco147I AGGCCT 1 cut(s) 202
Eco81I CCTNAGG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 221
FaeI CATG 1 cut(s) 148
FaiI YATR 3 cut(s) 9, 117, 146
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 239
FatI CATG 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 159, 218
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 159, 218
FspBI CTAG 1 cut(s) 198
GluI GCNGC 2 cut(s) 159, 218
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 2 cut(s) 202, 220
HapII CCGG 2 cut(s) 74, 263
Hin1II CATG 1 cut(s) 148
HinfI GANTC 1 cut(s) 227
HpaII CCGG 2 cut(s) 74, 263
HphI GGTGA 2 cut(s) 277, 280
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 1 cut(s) 35
Hpy8I GTNNAC 1 cut(s) 29
HpyCH4III ACNGT 3 cut(s) 26, 39, 92
HpyCH4V TGCA 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 199
HpyF3I CTNAG 2 cut(s) 32, 204
Hsp92II CATG 1 cut(s) 148
Kzo9I GATC 3 cut(s) 108, 247, 251
LpnPI CCDG 9 cut(s) 75, 87, 155, 168, 179, 208, 216, 235, 276
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 198
MalI GATC 3 cut(s) 110, 249, 253
MboI GATC 3 cut(s) 108, 247, 251
MboII GAAGA 2 cut(s) 118, 175
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 8 cut(s) 70, 76, 79, 119, 193, 199, 265, 296
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 120
MspI CCGG 2 cut(s) 74, 263
MspR9I CCNGG 2 cut(s) 223, 263
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 155, 199
NciI CCSGG 1 cut(s) 263
NdeII GATC 3 cut(s) 108, 247, 251
NlaIII CATG 1 cut(s) 148
PasI CCCWGGG 1 cut(s) 222
PceI AGGCCT 1 cut(s) 202
PkrI GCNGC 2 cut(s) 160, 219
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspPI GGNCC 1 cut(s) 219
RsaI GTAC 3 cut(s) 94, 140, 275
RsaNI GTAC 3 cut(s) 93, 139, 274
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 159, 218
Sau3AI GATC 3 cut(s) 108, 247, 251
Sau96I GGNCC 1 cut(s) 219
ScaI AGTACT 2 cut(s) 140, 275
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 223, 263
SetI ASST 6 cut(s) 81, 104, 130, 210, 244, 297
SfaNI GCATC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 120
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 3 cut(s) 49, 158, 217
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 221, 261
TaaI ACNGT 3 cut(s) 26, 39, 92
TaqI TCGA 1 cut(s) 250
TatI WGTACW 2 cut(s) 138, 273
TauI GCSGC 2 cut(s) 161, 220
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
XspI CTAG 1 cut(s) 198
ZrmI AGTACT 2 cut(s) 140, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.