Prupe.3G195300_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016899
Physical Location & Seq
Reverse (-)
20662923 .. 20664455
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G195300.1

Sequence Viewer

Length: 693 bp
ATGCTCCATAAGTTGCTTAATGTACATAAACTCAAGCCATTTGTGCGGCATGCGCTGCCTAAGCAAGCCCCGGCAACACAGCCAGAGTCATGGCCCGAGGACGGTGGTACTTCTACCTTGAAGATTGTCCATGCAGGTGGACGTGTTGAGAGCTACTACATGGCTATTCCTGCAGCCAAGATACTGGAGAAGTACCCCTCTTTCTTGCTAGCCAGGCCTGAAGTCTTCAGGCGGCCCTGGGATTCGGTAGTGAAGGCTGACGAGATCCTCACTCCTGGCCAAAAGGTCCTCCTAGTTCCTCGTCGAACTGTGAGGAAGCTCCGGCGCAGAATAAGAAAACCCAACAAAGAATTTTCTGTCAATTCTTATGTATCTCAGTCTTCCATGGACGTCTCAAGTCACACAATTTCGCAACGGAAACAAGGTGCAGTTGGTGAGCTGAGCGACTCTTCTTCTACCTGCAGCAGCATTGGAAGACAAAAAAGTGTTGGGAAGAAACATGTGACATTTACTGGCATTGATGTGAAGCACAGGTACAAGGCTGGTGAAATGGAGCACTCAACTTCAAGTGAGAGCTCAAGTTCTCAATCTCAAGGGAGGAAGAGAACGGTGAGAAGTGCCATGACATGGCAGCCCTCCCTTACAGCCATCACTGAAGCTCGGAGGGCTGATCATGTCATGAAATATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

231

Amino Acids

25.85

Weight (kDa)

10.32

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 125
AatII GACGTC 1 cut(s) 393
Acc36I ACCTGC 2 cut(s) 125, 467
AccB7I CCANNNNNTGG 1 cut(s) 627
AciI CCGC 2 cut(s) 46, 232
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 3 cut(s) 211, 240, 675
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 4 cut(s) 24, 109, 194, 536
AfiI CCNNNNNNNGG 2 cut(s) 101, 627
AflIII ACRYGT 2 cut(s) 142, 499
AgsI TTSAA 2 cut(s) 121, 567
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 3 cut(s) 212, 236, 274
AluBI AGCT 5 cut(s) 153, 319, 439, 576, 659
AluI AGCT 5 cut(s) 153, 319, 439, 576, 659
Alw21I GWGCWC 2 cut(s) 558, 578
Alw26I GTCTC 1 cut(s) 397
AlwI GGATC 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 4 cut(s) 92, 215, 233, 277
ApeKI GCWGC 5 cut(s) 55, 173, 462, 465, 631
ApoI RAATTY 1 cut(s) 350
AspLEI GCGC 2 cut(s) 55, 327
AspS9I GGNCC 3 cut(s) 93, 234, 286
AsuC2I CCSGG 1 cut(s) 71
AsuHPI GGTGA 3 cut(s) 446, 557, 622
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 279
BanII GRGCYC 1 cut(s) 578
BarI GAAGNNNNNNTAC 2 cut(s) 518, 550
BbsI GAAGAC 3 cut(s) 217, 372, 481
Bbv12I GWGCWC 2 cut(s) 558, 578
BbvI GCAGC 5 cut(s) 42, 185, 474, 477, 643
BccI CCATC 1 cut(s) 656
BciT130I CCWGG 3 cut(s) 214, 238, 276
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 1 cut(s) 71
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 2 cut(s) 209, 293
BfmI CTRYAG 2 cut(s) 171, 460
BfuAI ACCTGC 2 cut(s) 125, 467
BisI GCNGC 7 cut(s) 47, 56, 174, 233, 463, 466, 632
BlpI GCTNAGC 1 cut(s) 440
BlsI GCNGC 7 cut(s) 48, 57, 175, 234, 464, 467, 633
Bme1390I CCNGG 4 cut(s) 71, 214, 238, 276
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 93, 234, 286
BmrFI CCNGG 4 cut(s) 71, 214, 238, 276
BmtI GCTAGC 1 cut(s) 212
BpiI GAAGAC 3 cut(s) 217, 372, 481
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 440
BpuEI CTTGAG 4 cut(s) 17, 379, 562, 576
BpuMI CCSGG 1 cut(s) 71
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 5 cut(s) 69, 96, 236, 237, 384
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 627
Bse1I ACTGG 2 cut(s) 189, 517
BseBI CCWGG 3 cut(s) 214, 238, 276
BseDI CCNNGG 5 cut(s) 69, 96, 236, 237, 384
BseLI CCNNNNNNNGG 2 cut(s) 101, 627
BseMII CTCAG 2 cut(s) 389, 431
BseNI ACTGG 2 cut(s) 189, 517
BseXI GCAGC 5 cut(s) 42, 185, 474, 477, 643
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 4 cut(s) 94, 217, 235, 279
BsiHKAI GWGCWC 2 cut(s) 558, 578
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 71, 322
BslI CCNNNNNNNGG 2 cut(s) 101, 627
BsmAI GTCTC 1 cut(s) 397
BsmBI CGTCTC 1 cut(s) 397
BsnI GGCC 4 cut(s) 94, 217, 235, 279
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 558, 578
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 2 cut(s) 264, 670
Bsp1720I GCTNAGC 1 cut(s) 440
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 46, 232
BspANI GGCC 4 cut(s) 94, 217, 235, 279
BspCNI CTCAG 2 cut(s) 388, 432
BspHI TCATGA 1 cut(s) 678
BspMAI CTGCAG 2 cut(s) 175, 464
BspMI ACCTGC 2 cut(s) 125, 467
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 2 cut(s) 189, 517
BssECI CCNNGG 5 cut(s) 69, 96, 236, 237, 384
BssMI GATC 2 cut(s) 264, 670
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 214, 238, 276
Bst4CI ACNGT 3 cut(s) 104, 310, 610
Bst6I CTCTTC 2 cut(s) 454, 596
BstACI GRCGYC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 55
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 3 cut(s) 51, 66, 210
BstDEI CTNAG 3 cut(s) 60, 375, 440
BstDSI CCRYGG 1 cut(s) 384
BstHHI GCGC 2 cut(s) 55, 327
BstKTI GATC 2 cut(s) 267, 673
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 2 cut(s) 264, 670
BstMWI GCNNNNNNNGC 7 cut(s) 43, 52, 55, 61, 170, 214, 665
BstNI CCWGG 3 cut(s) 214, 238, 276
BstNSI RCATGY 2 cut(s) 53, 503
BstSCI CCNGG 4 cut(s) 69, 212, 236, 274
BstSFI CTRYAG 2 cut(s) 171, 460
BstV1I GCAGC 5 cut(s) 42, 185, 474, 477, 643
BstV2I GAAGAC 3 cut(s) 217, 372, 481
BstX2I RGATCY 1 cut(s) 264
BstXI CCANNNNNNTGG 3 cut(s) 90, 137, 184
BstYI RGATCY 1 cut(s) 264
BsuRI GGCC 4 cut(s) 94, 217, 235, 279
BtgI CCRYGG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 651
BveI ACCTGC 2 cut(s) 125, 467
Cac8I GCNNGC 3 cut(s) 51, 66, 210
CciI TCATGA 1 cut(s) 678
CfoI GCGC 2 cut(s) 55, 327
Cfr13I GGNCC 3 cut(s) 93, 234, 286
Csp6I GTAC 4 cut(s) 23, 108, 193, 535
CviQI GTAC 4 cut(s) 23, 108, 193, 535
DdeI CTNAG 3 cut(s) 60, 375, 440
DpnI GATC 2 cut(s) 266, 672
DpnII GATC 2 cut(s) 264, 670
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 454, 596
EarI CTCTTC 2 cut(s) 454, 596
Ecl136II GAGCTC 1 cut(s) 576
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 578
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 576
Eco57I CTGAAG 3 cut(s) 211, 240, 675
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 576
EcoO109I RGGNCCY 1 cut(s) 286
EcoRII CCWGG 3 cut(s) 212, 236, 274
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 397
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 7 cut(s) 47, 56, 174, 233, 463, 466, 632
FriOI GRGCYC 1 cut(s) 578
Fsp4HI GCNGC 7 cut(s) 47, 56, 174, 233, 463, 466, 632
FspBI CTAG 2 cut(s) 209, 293
GlaI GCGC 2 cut(s) 54, 326
GluI GCNGC 7 cut(s) 47, 56, 174, 233, 463, 466, 632
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 4 cut(s) 94, 217, 235, 279
HapII CCGG 2 cut(s) 71, 322
HhaI GCGC 2 cut(s) 55, 327
Hin1I GRCGYC 1 cut(s) 390
Hin6I GCGC 2 cut(s) 53, 325
HinP1I GCGC 2 cut(s) 53, 325
HinfI GANTC 3 cut(s) 86, 242, 446
HpaII CCGG 2 cut(s) 71, 322
HphI GGTGA 3 cut(s) 446, 557, 622
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 663
Hpy188III TCNNGA 1 cut(s) 679
Hpy8I GTNNAC 1 cut(s) 140
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 3 cut(s) 104, 310, 610
HpyCH4IV ACGT 2 cut(s) 142, 390
HpyCH4V TGCA 4 cut(s) 134, 173, 428, 462
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 52, 55, 61, 170, 214, 665
HpyF3I CTNAG 3 cut(s) 60, 375, 440
HpySE526I ACGT 2 cut(s) 142, 390
Hsp92I GRCGYC 1 cut(s) 390
HspAI GCGC 2 cut(s) 53, 325
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 2 cut(s) 264, 670
LmnI GCTCC 3 cut(s) 9, 324, 553
Lsp1109I GCAGC 5 cut(s) 42, 185, 474, 477, 643
MaeI CTAG 2 cut(s) 209, 293
MaeII ACGT 2 cut(s) 142, 390
MaeIII GTNAC 2 cut(s) 398, 502
MalI GATC 2 cut(s) 266, 672
MboI GATC 2 cut(s) 264, 670
MboII GAAGA 8 cut(s) 133, 217, 372, 441, 444, 486, 505, 613
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 2 cut(s) 558, 578
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 350, 361, 405
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 95, 440
MnlI CCTC 9 cut(s) 91, 208, 278, 299, 306, 309, 591, 646, 657
Mox20I TGGCCA 1 cut(s) 279
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 2 cut(s) 135, 521
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 2 cut(s) 71, 322
MspR9I CCNGG 4 cut(s) 71, 214, 238, 276
MvaI CCWGG 3 cut(s) 214, 238, 276
MwoI GCNNNNNNNGC 7 cut(s) 43, 52, 55, 61, 170, 214, 665
NciI CCSGG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 384
NdeII GATC 2 cut(s) 264, 670
NheI GCTAGC 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 398, 502
NspI RCATGY 2 cut(s) 53, 503
PaeI GCATGC 1 cut(s) 53
PagI TCATGA 1 cut(s) 678
PaqCI CACCTGC 1 cut(s) 125
PasI CCCWGGG 1 cut(s) 237
PceI AGGCCT 1 cut(s) 217
PciI ACATGT 1 cut(s) 499
PfeI GAWTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 627
PkrI GCNGC 7 cut(s) 48, 57, 175, 234, 464, 467, 633
PleI GAGTC 2 cut(s) 94, 440
PpsI GAGTC 2 cut(s) 94, 440
PpuMI RGGWCCY 1 cut(s) 286
PscI ACATGT 1 cut(s) 499
Psp124BI GAGCTC 1 cut(s) 578
Psp5II RGGWCCY 1 cut(s) 286
Psp6I CCWGG 3 cut(s) 212, 236, 274
PspGI CCWGG 3 cut(s) 212, 236, 274
PspPI GGNCC 3 cut(s) 93, 234, 286
PspPPI RGGWCCY 1 cut(s) 286
PstI CTGCAG 2 cut(s) 175, 464
PsuI RGATCY 1 cut(s) 264
RsaI GTAC 4 cut(s) 24, 109, 194, 536
RsaNI GTAC 4 cut(s) 23, 108, 193, 535
RseI CAYNNNNRTG 2 cut(s) 135, 521
SacI GAGCTC 1 cut(s) 578
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 7 cut(s) 47, 56, 174, 233, 463, 466, 632
Sau3AI GATC 2 cut(s) 264, 670
Sau96I GGNCC 3 cut(s) 93, 234, 286
SchI GAGTC 2 cut(s) 95, 440
ScrFI CCNGG 4 cut(s) 71, 214, 238, 276
SduI GDGCHC 2 cut(s) 558, 578
SfcI CTRYAG 2 cut(s) 171, 460
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 135, 521
SmlI CTYRAG 4 cut(s) 32, 394, 577, 591
SmoI CTYRAG 4 cut(s) 32, 394, 577, 591
SphI GCATGC 1 cut(s) 53
Sse9I AATT 3 cut(s) 350, 361, 405
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 2 cut(s) 46, 232
SspMI CTAG 2 cut(s) 209, 293
SstI GAGCTC 1 cut(s) 578
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 4 cut(s) 69, 212, 236, 274
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 3 cut(s) 104, 310, 610
TaiI ACGT 2 cut(s) 145, 393
TaqI TCGA 1 cut(s) 304
TasI AATT 3 cut(s) 350, 361, 405
TatI WGTACW 1 cut(s) 22
TauI GCSGC 2 cut(s) 49, 235
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 1 cut(s) 658
TseFI GTSAC 2 cut(s) 398, 502
TseI GCWGC 5 cut(s) 55, 173, 462, 465, 631
Tsp45I GTSAC 2 cut(s) 398, 502
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 1 cut(s) 658
Van91I CCANNNNNTGG 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 1 cut(s) 350
XceI RCATGY 2 cut(s) 53, 503
XspI CTAG 2 cut(s) 209, 293
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.