Rroxscaffold_2G00093570

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016899
Physical Location & Seq
Forward (+)
14921688 .. 14922446
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00093570.1

Sequence Viewer

Length: 759 bp
ATGTCCGGTACTTGGAAGAACTATACCCAAGTTGCCATGCTCCACAAGTTCGTCAATATCGGTAAACTTAAGCCATTCTCAAAGCGTGTGCCTGTGCCGGTGCCGCCTAAGCAAGCTCCGGCACAGTCAGAGACGGGCAGCAACTCTATTTTGAAGGTTGTTCACGCTGGTGGAAATGTTGAGAGTTACTACATGGCTATTCCTGCAGCCAATATTTTGGAGAAGTACCCTTCTTTCTTGCTCGCTAGGCCTGAAGTCTTTAGGCGGCCGTGGGACTCGGTCGTGAAGCCGGATGCAATACTCACACCCGGAGAGAAAGTCTTTCTTGTTCCCCGCAGGACTGTGAGGAAGCTCCGTCGCAGAATACGAAGACCCGGGAAGGATTTTACAGTCAATTCTTTCATGTCTCAATCGTCTATTGATGTATCAACTGAGACTCTACCACAGAGGAGAGATGTCGGTAGTGCAGTTGGTGATGCAAGTGAGAGTGACGCTTCTATTTGTAGTGCTATTAGCAGAAACAAAGGTGATGGCAAGAAGCATGTGACTTTCAGTGGCATCAAGAAACATGTGACATTTGCTGGCCTTGATGTGAAGCACAAACCGGAGAAGACTCGCAGCGAAATCGAGGAGCAAGATTCGAGTGAAAGCTCGAGTTCTCTAAAATCTCTCGGAGGGAGAAGGAGGGTACGGGTTGTGACATGGCAGCCGACTCTTACAGCAATAACAGAAGCTCATGGGAATGATTTGATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

252

Amino Acids

27.92

Weight (kDa)

10.19

Isoelectric Point (pI)

75.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 43 - 244 2e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AciI CCGC 3 cut(s) 104, 265, 334
AcoI YGGCCR 1 cut(s) 266
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 3 cut(s) 10, 227, 690
AfiI CCNNNNNNNGG 1 cut(s) 12
AflII CTTAAG 1 cut(s) 68
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 168
AluBI AGCT 4 cut(s) 116, 352, 651, 734
AluI AGCT 4 cut(s) 116, 352, 651, 734
Alw26I GTCTC 3 cut(s) 125, 411, 428
Ama87I CYCGRG 2 cut(s) 374, 652
AoxI GGCC 3 cut(s) 248, 266, 583
ApeKI GCWGC 4 cut(s) 138, 206, 618, 706
AsuC2I CCSGG 3 cut(s) 309, 375, 376
AsuHPI GGTGA 2 cut(s) 485, 539
AvaI CYCGRG 2 cut(s) 374, 652
BanI GGYRCC 1 cut(s) 100
BbsI GAAGAC 2 cut(s) 376, 617
BbvI GCAGC 4 cut(s) 150, 218, 630, 718
BccI CCATC 1 cut(s) 524
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 607, 641
BcnI CCSGG 3 cut(s) 309, 375, 376
BcoDI GTCTC 3 cut(s) 125, 411, 428
BfaI CTAG 1 cut(s) 246
BfmI CTRYAG 1 cut(s) 204
BfrI CTTAAG 1 cut(s) 68
BisI GCNGC 6 cut(s) 104, 139, 207, 266, 619, 707
BlsI GCNGC 6 cut(s) 105, 140, 208, 267, 620, 708
Bme1390I CCNGG 3 cut(s) 309, 375, 376
BmeT110I CYCGRG 2 cut(s) 374, 652
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 3 cut(s) 309, 375, 376
BmsI GCATC 3 cut(s) 283, 466, 567
BpiI GAAGAC 2 cut(s) 376, 617
Bpu10I CCTNAGC 1 cut(s) 108
BpuMI CCSGG 3 cut(s) 309, 375, 376
BsaJI CCNNGG 2 cut(s) 269, 374
BsaWI WCCGGW 2 cut(s) 5, 604
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse118I RCCGGY 1 cut(s) 97
BseDI CCNNGG 2 cut(s) 269, 374
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 423
BseRI GAGGAG 2 cut(s) 463, 644
BseX3I CGGCCG 1 cut(s) 266
BseXI GCAGC 4 cut(s) 150, 218, 630, 718
BsgI GTGCAG 1 cut(s) 486
Bsh1285I CGRYCG 2 cut(s) 269, 282
BshFI GGCC 3 cut(s) 250, 268, 585
BshNI GGYRCC 1 cut(s) 100
BsiEI CGRYCG 2 cut(s) 269, 282
BsiHKCI CYCGRG 2 cut(s) 374, 652
BsiSI CCGG 7 cut(s) 6, 98, 119, 290, 309, 375, 605
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 12
BsmAI GTCTC 3 cut(s) 125, 411, 428
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 287
BsnI GGCC 3 cut(s) 250, 268, 585
BsoBI CYCGRG 2 cut(s) 374, 652
BspACI CCGC 3 cut(s) 104, 265, 334
BspANI GGCC 3 cut(s) 250, 268, 585
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 100
BspTI CTTAAG 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 269, 374
Bst4CI ACNGT 3 cut(s) 126, 343, 391
BstAFI CTTAAG 1 cut(s) 68
BstC8I GCNNGC 3 cut(s) 114, 243, 583
BstDEI CTNAG 2 cut(s) 108, 432
BstDSI CCRYGG 1 cut(s) 269
BstF5I GGATG 1 cut(s) 298
BstMAI GTCTC 3 cut(s) 125, 411, 428
BstMCI CGRYCG 2 cut(s) 269, 282
BstMWI GCNNNNNNNGC 4 cut(s) 103, 109, 203, 247
BstNSI RCATGY 2 cut(s) 545, 572
BstSCI CCNGG 3 cut(s) 307, 373, 374
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 4 cut(s) 150, 218, 630, 718
BstV2I GAAGAC 2 cut(s) 376, 617
BstXI CCANNNNNNTGG 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 266
BsuRI GGCC 3 cut(s) 250, 268, 585
BtgI CCRYGG 1 cut(s) 269
BtsCI GGATG 1 cut(s) 298
BtsIMutI CAGTG 1 cut(s) 559
Cac8I GCNNGC 3 cut(s) 114, 243, 583
Cfr10I RCCGGY 1 cut(s) 97
Cfr9I CCCGGG 1 cut(s) 374
CseI GACGC 1 cut(s) 500
Csp6I GTAC 3 cut(s) 9, 226, 689
CviAII CATG 7 cut(s) 37, 193, 403, 542, 569, 702, 737
CviQI GTAC 3 cut(s) 9, 226, 689
DdeI CTNAG 2 cut(s) 108, 432
EaeI YGGCCR 1 cut(s) 266
EagI CGGCCG 1 cut(s) 266
EclXI CGGCCG 1 cut(s) 266
Eco147I AGGCCT 1 cut(s) 250
Eco52I CGGCCG 1 cut(s) 266
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 374, 652
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 7 cut(s) 40, 196, 406, 545, 572, 705, 740
FaiI YATR 8 cut(s) 24, 38, 194, 404, 543, 570, 703, 738
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 287
FatI CATG 7 cut(s) 36, 192, 402, 541, 568, 701, 736
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 6 cut(s) 104, 139, 207, 266, 619, 707
FokI GGATG 1 cut(s) 305
Fsp4HI GCNGC 6 cut(s) 104, 139, 207, 266, 619, 707
FspBI CTAG 1 cut(s) 246
GluI GCNGC 6 cut(s) 104, 139, 207, 266, 619, 707
HaeIII GGCC 3 cut(s) 250, 268, 585
HapII CCGG 7 cut(s) 6, 98, 119, 290, 309, 375, 605
HgaI GACGC 1 cut(s) 500
Hin1II CATG 7 cut(s) 40, 196, 406, 545, 572, 705, 740
HinfI GANTC 5 cut(s) 275, 436, 613, 638, 712
HpaII CCGG 7 cut(s) 6, 98, 119, 290, 309, 375, 605
HphI GGTGA 2 cut(s) 485, 539
Hpy166II GTNNAC 2 cut(s) 65, 163
Hpy188I TCNGA 2 cut(s) 130, 674
Hpy188III TCNNGA 2 cut(s) 283, 562
Hpy8I GTNNAC 2 cut(s) 65, 163
Hpy99I CGWCG 1 cut(s) 360
HpyAV CCTTC 4 cut(s) 148, 240, 373, 675
HpyCH4III ACNGT 3 cut(s) 126, 343, 391
HpyCH4V TGCA 4 cut(s) 206, 296, 467, 479
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 109, 203, 247
HpyF3I CTNAG 2 cut(s) 108, 432
Hsp92II CATG 7 cut(s) 40, 196, 406, 545, 572, 705, 740
LmnI GCTCC 4 cut(s) 45, 121, 357, 631
Lsp1109I GCAGC 4 cut(s) 150, 218, 630, 718
LweI GCATC 3 cut(s) 283, 466, 567
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 5 cut(s) 185, 488, 544, 571, 697
MboII GAAGA 3 cut(s) 28, 381, 622
MluCI AATT 1 cut(s) 394
MlyI GAGTC 4 cut(s) 269, 430, 607, 706
MnlI CCTC 5 cut(s) 339, 441, 622, 668, 678
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 2 cut(s) 168, 741
MspCI CTTAAG 1 cut(s) 68
MspI CCGG 7 cut(s) 6, 98, 119, 290, 309, 375, 605
MspR9I CCNGG 3 cut(s) 309, 375, 376
MwoI GCNNNNNNNGC 4 cut(s) 103, 109, 203, 247
NciI CCSGG 3 cut(s) 309, 375, 376
NlaIII CATG 7 cut(s) 40, 196, 406, 545, 572, 705, 740
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 4 cut(s) 488, 544, 571, 697
NspI RCATGY 2 cut(s) 545, 572
OliI CACNNNNGTG 1 cut(s) 168
PaeR7I CTCGAG 1 cut(s) 652
PceI AGGCCT 1 cut(s) 250
PciI ACATGT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 364
PfeI GAWTC 1 cut(s) 638
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 6 cut(s) 105, 140, 208, 267, 620, 708
PleI GAGTC 4 cut(s) 269, 430, 607, 706
PpsI GAGTC 4 cut(s) 269, 430, 607, 706
PscI ACATGT 1 cut(s) 568
PspN4I GGNNCC 1 cut(s) 102
PspXI VCTCGAGB 1 cut(s) 652
PstI CTGCAG 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 10, 227, 690
RsaNI GTAC 3 cut(s) 9, 226, 689
RseI CAYNNNNRTG 2 cut(s) 168, 741
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 6 cut(s) 104, 139, 207, 266, 619, 707
SchI GAGTC 4 cut(s) 269, 430, 607, 706
ScrFI CCNGG 3 cut(s) 309, 375, 376
SetI ASST 6 cut(s) 118, 159, 354, 529, 653, 736
SfaNI GCATC 3 cut(s) 283, 466, 567
SfcI CTRYAG 1 cut(s) 204
Sfr274I CTCGAG 1 cut(s) 652
SlaI CTCGAG 1 cut(s) 652
SmaI CCCGGG 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 168, 741
SmlI CTYRAG 2 cut(s) 68, 652
SmoI CTYRAG 2 cut(s) 68, 652
Sse9I AATT 1 cut(s) 394
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 3 cut(s) 104, 265, 334
SspI AATATT 1 cut(s) 214
SspMI CTAG 1 cut(s) 246
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 3 cut(s) 307, 373, 374
TaaI ACNGT 3 cut(s) 126, 343, 391
TaqI TCGA 3 cut(s) 627, 641, 653
TaqII GACCGA 1 cut(s) 268
TasI AATT 1 cut(s) 394
TauI GCSGC 2 cut(s) 106, 268
TfiI GAWTC 1 cut(s) 638
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 4 cut(s) 488, 544, 571, 697
TseI GCWGC 4 cut(s) 138, 206, 618, 706
Tsp45I GTSAC 4 cut(s) 488, 544, 571, 697
TspDTI ATGAA 1 cut(s) 391
TspGWI ACGGA 1 cut(s) 344
TspMI CCCGGG 1 cut(s) 374
TspRI CASTG 1 cut(s) 559
Tth111I GACNNNGTC 1 cut(s) 278
Vha464I CTTAAG 1 cut(s) 68
XceI RCATGY 2 cut(s) 545, 572
XhoI CTCGAG 1 cut(s) 652
XmaI CCCGGG 1 cut(s) 374
XspI CTAG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.