Rh2BG526200

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016899
Physical Location & Seq
Reverse (-)
73592231 .. 73592998
768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG526200.1

Sequence Viewer

Length: 768 bp
ATGTCCAGTACTTTGCAGAAGAACTATAGCCAAGTTGTCATGCTCCACAAATTCCTCAATATCAGTAAACTTAAGCCATTCTCAAGGCGTGTGCCTGTGCCTGTGCCGGTGCCGCCTAAGCAAGCTCCGGCACAGTCAGAGACGGGCAGCAACTCTATTTTGAAGGTTGTTCACGCAGGTGGAAATGTTGAGAGTTACTACATGGCTATTCCTGCAGCCAATATTTTGGAGAAGTACCCTTCTTTCTTGCTCGCTAGGCCTGAAGTCTTCAGGCGGCCGTGGGACTCAGTAGTGAAGCCGGATGCAATACTCACACCCGGAGAGAAAGTCTTTCTTGTTCCCCGCAGGACTGTGAGGAAGCTCCGTCGCAGAATACGAAGACCCGGGAAGGATTTTTCAGTCAATTGTTTCATGTCTCAATCGTCTATTGATGTATCAACTGAGACTCTACCACAGAGGAGAGATGTCGGTAGTGCAGTTGGTGATGCAAGTGAGAGTGACGCTTCTATTTGTAGTGCTATTAGCAGAAACAAAAGTGATGGCAAGAAGCATGTGACTTTTCGTGGCATCAAGAAACATGTGACATTTGCTGGCCTTGATGTGAAGCACAGGCCGGAGAAGACTCACAGCGAAATCGAGGAGCAAGATTCGAGTGAAAGCTCGAGTTCTCTAAAATCTCTCCGAGGGAGAAGGAGGGTACGGGTTGTGACATGGCAGCCGATTCTTACAGCAATAACAGAAGCTCATGGGAATGATTTGATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

255

Amino Acids

28.51

Weight (kDa)

10.27

Isoelectric Point (pI)

81.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 46 - 247 7.5e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 109
AciI CCGC 3 cut(s) 113, 274, 343
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 2 cut(s) 253, 282
AfaI GTAC 3 cut(s) 10, 236, 699
AflII CTTAAG 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 1 cut(s) 163
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 4 cut(s) 125, 361, 660, 743
AluI AGCT 4 cut(s) 125, 361, 660, 743
Alw26I GTCTC 3 cut(s) 134, 420, 437
Ama87I CYCGRG 2 cut(s) 383, 661
AoxI GGCC 4 cut(s) 257, 275, 592, 611
ApeKI GCWGC 3 cut(s) 147, 215, 715
ApoI RAATTY 1 cut(s) 50
AsuC2I CCSGG 3 cut(s) 318, 384, 385
AsuHPI GGTGA 1 cut(s) 494
AvaI CYCGRG 2 cut(s) 383, 661
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 3 cut(s) 259, 385, 626
BbvI GCAGC 3 cut(s) 159, 227, 727
BccI CCATC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 262
BcnI CCSGG 3 cut(s) 318, 384, 385
BcoDI GTCTC 3 cut(s) 134, 420, 437
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 2 cut(s) 25, 213
BfrI CTTAAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 167
BisI GCNGC 5 cut(s) 113, 148, 216, 275, 716
BlsI GCNGC 5 cut(s) 114, 149, 217, 276, 717
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 318, 384, 385
BmeT110I CYCGRG 2 cut(s) 383, 661
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 3 cut(s) 318, 384, 385
BmsI GCATC 3 cut(s) 292, 475, 576
BpiI GAAGAC 3 cut(s) 259, 385, 626
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 67
BpuMI CCSGG 3 cut(s) 318, 384, 385
BsaJI CCNNGG 3 cut(s) 278, 383, 682
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 278, 383, 682
BseGI GGATG 1 cut(s) 307
BseMII CTCAG 2 cut(s) 300, 432
BseNI ACTGG 1 cut(s) 6
BseRI GAGGAG 2 cut(s) 472, 653
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 3 cut(s) 159, 227, 727
BsgI GTGCAG 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 4 cut(s) 259, 277, 594, 613
BshNI GGYRCC 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 278
BsiHKCI CYCGRG 2 cut(s) 383, 661
BsiSI CCGG 6 cut(s) 107, 128, 299, 318, 384, 614
BslFI GGGAC 1 cut(s) 296
BsmAI GTCTC 3 cut(s) 134, 420, 437
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 4 cut(s) 259, 277, 594, 613
BsoBI CYCGRG 2 cut(s) 383, 661
BspACI CCGC 3 cut(s) 113, 274, 343
BspANI GGCC 4 cut(s) 259, 277, 594, 613
BspCNI CTCAG 2 cut(s) 299, 433
BspLI GGNNCC 1 cut(s) 111
BspMAI CTGCAG 1 cut(s) 217
BspMI ACCTGC 1 cut(s) 167
BspT107I GGYRCC 1 cut(s) 109
BspTI CTTAAG 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 1 cut(s) 6
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 3 cut(s) 278, 383, 682
Bst4CI ACNGT 2 cut(s) 135, 352
BstAFI CTTAAG 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 123, 252, 592
BstDEI CTNAG 3 cut(s) 117, 286, 441
BstDSI CCRYGG 1 cut(s) 278
BstF5I GGATG 1 cut(s) 307
BstMAI GTCTC 3 cut(s) 134, 420, 437
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 4 cut(s) 112, 118, 212, 256
BstNSI RCATGY 2 cut(s) 554, 581
BstSCI CCNGG 3 cut(s) 316, 382, 383
BstSFI CTRYAG 2 cut(s) 25, 213
BstV1I GCAGC 3 cut(s) 159, 227, 727
BstV2I GAAGAC 3 cut(s) 259, 385, 626
BstXI CCANNNNNNTGG 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 275
BsuRI GGCC 4 cut(s) 259, 277, 594, 613
BtgI CCRYGG 1 cut(s) 278
BtsCI GGATG 1 cut(s) 307
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 3 cut(s) 123, 252, 592
Cfr10I RCCGGY 1 cut(s) 106
Cfr9I CCCGGG 1 cut(s) 383
CseI GACGC 1 cut(s) 509
Csp6I GTAC 3 cut(s) 9, 235, 698
CviAII CATG 7 cut(s) 40, 202, 412, 551, 578, 711, 746
CviQI GTAC 3 cut(s) 9, 235, 698
DdeI CTNAG 3 cut(s) 117, 286, 441
EaeI YGGCCR 1 cut(s) 275
EagI CGGCCG 1 cut(s) 275
EclXI CGGCCG 1 cut(s) 275
Eco147I AGGCCT 1 cut(s) 259
Eco52I CGGCCG 1 cut(s) 275
Eco57I CTGAAG 2 cut(s) 253, 282
Eco88I CYCGRG 2 cut(s) 383, 661
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 7 cut(s) 43, 205, 415, 554, 581, 714, 749
FaiI YATR 8 cut(s) 27, 41, 203, 413, 552, 579, 712, 747
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 296
FatI CATG 7 cut(s) 39, 201, 411, 550, 577, 710, 745
FauI CCCGC 1 cut(s) 350
Fnu4HI GCNGC 5 cut(s) 113, 148, 216, 275, 716
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 5 cut(s) 113, 148, 216, 275, 716
FspBI CTAG 1 cut(s) 255
GluI GCNGC 5 cut(s) 113, 148, 216, 275, 716
HaeIII GGCC 4 cut(s) 259, 277, 594, 613
HapII CCGG 6 cut(s) 107, 128, 299, 318, 384, 614
HgaI GACGC 1 cut(s) 509
Hin1II CATG 7 cut(s) 43, 205, 415, 554, 581, 714, 749
HinfI GANTC 5 cut(s) 284, 445, 622, 647, 721
HpaII CCGG 6 cut(s) 107, 128, 299, 318, 384, 614
HphI GGTGA 1 cut(s) 494
Hpy166II GTNNAC 2 cut(s) 68, 172
Hpy188I TCNGA 2 cut(s) 139, 683
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 2 cut(s) 68, 172
Hpy99I CGWCG 1 cut(s) 369
HpyAV CCTTC 4 cut(s) 157, 249, 382, 684
HpyCH4III ACNGT 2 cut(s) 135, 352
HpyCH4V TGCA 5 cut(s) 16, 215, 305, 476, 488
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 118, 212, 256
HpyF3I CTNAG 3 cut(s) 117, 286, 441
Hsp92II CATG 7 cut(s) 43, 205, 415, 554, 581, 714, 749
LmnI GCTCC 4 cut(s) 48, 130, 366, 640
Lsp1109I GCAGC 3 cut(s) 159, 227, 727
LweI GCATC 3 cut(s) 292, 475, 576
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 5 cut(s) 194, 497, 553, 580, 706
MboII GAAGA 4 cut(s) 31, 259, 390, 631
MfeI CAATTG 1 cut(s) 403
MluCI AATT 2 cut(s) 50, 403
MlyI GAGTC 3 cut(s) 278, 439, 616
MnlI CCTC 6 cut(s) 65, 348, 450, 631, 677, 687
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 2 cut(s) 177, 750
MspCI CTTAAG 1 cut(s) 71
MspI CCGG 6 cut(s) 107, 128, 299, 318, 384, 614
MspR9I CCNGG 3 cut(s) 318, 384, 385
MunI CAATTG 1 cut(s) 403
MwoI GCNNNNNNNGC 4 cut(s) 112, 118, 212, 256
NciI CCSGG 3 cut(s) 318, 384, 385
NlaIII CATG 7 cut(s) 43, 205, 415, 554, 581, 714, 749
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 4 cut(s) 497, 553, 580, 706
NspI RCATGY 2 cut(s) 554, 581
OliI CACNNNNGTG 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 661
PaqCI CACCTGC 1 cut(s) 167
PceI AGGCCT 1 cut(s) 259
PciI ACATGT 1 cut(s) 577
PcsI WCGNNNNNNNCGW 1 cut(s) 373
PfeI GAWTC 2 cut(s) 647, 721
PkrI GCNGC 5 cut(s) 114, 149, 217, 276, 717
PleI GAGTC 3 cut(s) 278, 439, 616
PpsI GAGTC 3 cut(s) 278, 439, 616
PscI ACATGT 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 111
PspXI VCTCGAGB 1 cut(s) 661
PstI CTGCAG 1 cut(s) 217
RsaI GTAC 3 cut(s) 10, 236, 699
RsaNI GTAC 3 cut(s) 9, 235, 698
RseI CAYNNNNRTG 2 cut(s) 177, 750
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 5 cut(s) 113, 148, 216, 275, 716
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 3 cut(s) 278, 439, 616
ScrFI CCNGG 3 cut(s) 318, 384, 385
SetI ASST 6 cut(s) 127, 168, 181, 363, 662, 745
SfaNI GCATC 3 cut(s) 292, 475, 576
SfcI CTRYAG 2 cut(s) 25, 213
Sfr274I CTCGAG 1 cut(s) 661
SlaI CTCGAG 1 cut(s) 661
SmaI CCCGGG 1 cut(s) 385
SmiMI CAYNNNNRTG 2 cut(s) 177, 750
SmlI CTYRAG 3 cut(s) 71, 82, 661
SmoI CTYRAG 3 cut(s) 71, 82, 661
Sse9I AATT 2 cut(s) 50, 403
SseBI AGGCCT 1 cut(s) 259
SsiI CCGC 3 cut(s) 113, 274, 343
SspI AATATT 1 cut(s) 223
SspMI CTAG 1 cut(s) 255
StuI AGGCCT 1 cut(s) 259
StyD4I CCNGG 3 cut(s) 316, 382, 383
TaaI ACNGT 2 cut(s) 135, 352
TaqI TCGA 3 cut(s) 636, 650, 662
TasI AATT 2 cut(s) 50, 403
TatI WGTACW 1 cut(s) 8
TauI GCSGC 2 cut(s) 115, 277
TfiI GAWTC 2 cut(s) 647, 721
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseFI GTSAC 4 cut(s) 497, 553, 580, 706
TseI GCWGC 3 cut(s) 147, 215, 715
Tsp45I GTSAC 4 cut(s) 497, 553, 580, 706
TspDTI ATGAA 1 cut(s) 400
TspGWI ACGGA 1 cut(s) 353
TspMI CCCGGG 1 cut(s) 383
Vha464I CTTAAG 1 cut(s) 71
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 554, 581
XhoI CTCGAG 1 cut(s) 661
XmaI CCCGGG 1 cut(s) 383
XspI CTAG 1 cut(s) 255
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.