MD09G1129900.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016899
Physical Location & Seq
Forward (+)
10022355 .. 10023026
672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1129900.v1.1.491

Sequence Viewer

Length: 672 bp
ATGTTCCACAAGTTCCTCAACGGCCATAAACTCAAACCGTTCTCAAGGCGGCCGCAACCTCCGGAAGCAGAGCCGGAGGTGCAGGAGGACGGTACTTCTATCTTGAAGATTGTCCATATCGGTGGAGGTGTTGAGAAGCACTACATGGCAACTCCTGCGGCGAAGATACTGGAGAAGCATCCCTCTTTCTTGCTGGCTAGGCCTGAGGTTTTTAGGCGGCCCTGGGACTCGGTAGTTAAACCTAATGAGATCCTCACGCCCGGTGAGAAACTACTCCTTATTCCTCACCGAGCTGTGAGGAAGCTCCGGCGTAGAATAAGAAAACCTAACAAAGAGCTTTCTGTCAACTCTGTTGCGTCTCAATCTTCCATGGATGTCTCAACCGCGGCCGAGACAATTTTGCGCTGGAAACAAGGTGCGGCGGTTGGTGAGCTAAGTGACACTTCTATTTGTAGTATTATCGGAAGAAAGAACGGTGTTGGCAAGAAACATGTGACATTCCCTGGCATTGATGTCAAGCACAAAGGTGGTGGAACCGAGCGTTCGAGTAAAAGCTCGAGCGCTCAATCTCATCGAGGGAAGATGAGGGTCCGAGGTTCCATGACATGGCAGCCGTCGCTTACAACGATCACAGAAACTCGTGGGAATGATCATGTGAAGAAATATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

224

Amino Acids

24.86

Weight (kDa)

10.35

Isoelectric Point (pI)

59.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 33 - 212 3.4e-15 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 606
AccII CGCG 1 cut(s) 386
AccIII TCCGGA 1 cut(s) 61
AciI CCGC 8 cut(s) 49, 53, 158, 217, 384, 386, 419, 422
AclWI GGATC 1 cut(s) 244
AcoI YGGCCR 3 cut(s) 22, 50, 387
AfaI GTAC 1 cut(s) 94
AfeI AGCGCT 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 606
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 2 cut(s) 221, 502
AleI CACNNNNGTG 1 cut(s) 525
AluBI AGCT 5 cut(s) 293, 304, 337, 433, 555
AluI AGCT 5 cut(s) 293, 304, 337, 433, 555
Alw26I GTCTC 3 cut(s) 363, 382, 386
AlwI GGATC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 556
Aor13HI TCCGGA 1 cut(s) 61
Aor51HI AGCGCT 1 cut(s) 562
AoxI GGCC 5 cut(s) 22, 50, 200, 218, 387
ApeKI GCWGC 1 cut(s) 610
AspLEI GCGC 2 cut(s) 405, 563
AspS9I GGNCC 2 cut(s) 219, 589
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 3 cut(s) 275, 278, 440
AvaI CYCGRG 1 cut(s) 556
AvaII GGWCC 1 cut(s) 589
AxyI CCTNAGG 1 cut(s) 204
BauI CACGAG 1 cut(s) 639
BbvI GCAGC 1 cut(s) 622
BceAI ACGGC 2 cut(s) 37, 598
BciT130I CCWGG 2 cut(s) 223, 504
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 3 cut(s) 363, 382, 386
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 564
BisI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 611
BlsI GCNGC 7 cut(s) 51, 54, 160, 219, 388, 421, 612
Bme1390I CCNGG 3 cut(s) 223, 261, 504
Bme18I GGWCC 1 cut(s) 589
BmeT110I CYCGRG 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 219, 589
BmiI GGNNCC 3 cut(s) 535, 590, 598
BmrFI CCNGG 3 cut(s) 223, 261, 504
BmsI GCATC 1 cut(s) 187
BpmI CTGGAG 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 261
BsaJI CCNNGG 6 cut(s) 221, 222, 369, 384, 502, 592
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse1I ACTGG 1 cut(s) 174
Bse21I CCTNAGG 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 2 cut(s) 223, 504
BseDI CCNNGG 6 cut(s) 221, 222, 369, 384, 502, 592
BseGI GGATG 2 cut(s) 178, 379
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 1 cut(s) 174
BseX3I CGGCCG 2 cut(s) 50, 387
BseXI GCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 386
Bsh1285I CGRYCG 2 cut(s) 53, 390
BshFI GGCC 5 cut(s) 24, 52, 202, 220, 389
BsiEI CGRYCG 2 cut(s) 53, 390
BsiHKCI CYCGRG 1 cut(s) 556
BsiSI CCGG 4 cut(s) 62, 74, 261, 307
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 3 cut(s) 363, 382, 386
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 5 cut(s) 24, 52, 202, 220, 389
BsoBI CYCGRG 1 cut(s) 556
Bsp13I TCCGGA 1 cut(s) 61
Bsp143I GATC 3 cut(s) 249, 627, 649
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 8 cut(s) 49, 53, 158, 217, 384, 386, 419, 422
BspANI GGCC 5 cut(s) 24, 52, 202, 220, 389
BspCNI CTCAG 1 cut(s) 196
BspEI TCCGGA 1 cut(s) 61
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 3 cut(s) 535, 590, 598
BspPI GGATC 1 cut(s) 244
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 6 cut(s) 221, 222, 369, 384, 502, 592
BssMI GATC 3 cut(s) 249, 627, 649
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 223, 504
Bst4CI ACNGT 3 cut(s) 39, 92, 476
BstAPI GCANNNNNTGC 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 204, 434
BstDSI CCRYGG 2 cut(s) 369, 384
BstF5I GGATG 2 cut(s) 178, 379
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 2 cut(s) 405, 563
BstKTI GATC 3 cut(s) 252, 630, 652
BstMAI GTCTC 3 cut(s) 363, 382, 386
BstMBI GATC 3 cut(s) 249, 627, 649
BstMCI CGRYCG 2 cut(s) 53, 390
BstMWI GCNNNNNNNGC 4 cut(s) 79, 155, 199, 616
BstNI CCWGG 2 cut(s) 223, 504
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 221, 259, 502
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 622
BstX2I RGATCY 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 249
BstZI CGGCCG 2 cut(s) 50, 387
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 5 cut(s) 24, 52, 202, 220, 389
BtgI CCRYGG 2 cut(s) 369, 384
BtsCI GGATG 2 cut(s) 178, 379
Cac8I GCNNGC 1 cut(s) 195
CciNI GCGGCCGC 1 cut(s) 50
CfoI GCGC 2 cut(s) 405, 563
Cfr13I GGNCC 2 cut(s) 219, 589
Cfr42I CCGCGG 1 cut(s) 387
CseI GACGC 1 cut(s) 345
Csp6I GTAC 1 cut(s) 93
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 6 cut(s) 145, 370, 491, 601, 606, 653
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 2 cut(s) 204, 434
DpnI GATC 3 cut(s) 251, 629, 651
DpnII GATC 3 cut(s) 249, 627, 649
EaeI YGGCCR 3 cut(s) 22, 50, 387
EagI CGGCCG 2 cut(s) 50, 387
EclXI CGGCCG 2 cut(s) 50, 387
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 589
Eco47III AGCGCT 1 cut(s) 562
Eco52I CGGCCG 2 cut(s) 50, 387
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 221, 502
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 6 cut(s) 148, 373, 494, 604, 609, 656
FaiI YATR 9 cut(s) 27, 117, 146, 371, 492, 602, 607, 654, 666
FalI AAGNNNNNCTT 2 cut(s) 427, 459
FaqI GGGAC 1 cut(s) 239
FatI CATG 6 cut(s) 144, 369, 490, 600, 605, 652
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 611
FokI GGATG 2 cut(s) 165, 386
Fsp4HI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 611
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 404, 562
GluI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 611
GsuI CTGGAG 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 5 cut(s) 24, 52, 202, 220, 389
HapII CCGG 4 cut(s) 62, 74, 261, 307
HgaI GACGC 1 cut(s) 345
HhaI GCGC 2 cut(s) 405, 563
Hin1II CATG 6 cut(s) 148, 373, 494, 604, 609, 656
Hin6I GCGC 2 cut(s) 403, 561
HinP1I GCGC 2 cut(s) 403, 561
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 1 cut(s) 227
HpaII CCGG 4 cut(s) 62, 74, 261, 307
HphI GGTGA 3 cut(s) 275, 278, 440
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 464, 593
Hpy188III TCNNGA 2 cut(s) 62, 103
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 619
HpyCH4III ACNGT 3 cut(s) 39, 92, 476
HpyCH4V TGCA 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 155, 199, 616
HpyF3I CTNAG 2 cut(s) 204, 434
Hsp92II CATG 6 cut(s) 148, 373, 494, 604, 609, 656
HspAI GCGC 2 cut(s) 403, 561
Kpn2I TCCGGA 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 649
KspI CCGCGG 1 cut(s) 387
Kzo9I GATC 3 cut(s) 249, 627, 649
LmnI GCTCC 1 cut(s) 309
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 1 cut(s) 187
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 437, 493
MalI GATC 3 cut(s) 251, 629, 651
MboI GATC 3 cut(s) 249, 627, 649
MboII GAAGA 6 cut(s) 118, 175, 357, 477, 592, 670
MflI RGATCY 1 cut(s) 249
MluCI AATT 1 cut(s) 396
MlyI GAGTC 1 cut(s) 221
MroI TCCGGA 1 cut(s) 61
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 2 cut(s) 120, 525
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 62, 74, 261, 307
MspR9I CCNGG 3 cut(s) 223, 261, 504
MvaI CCWGG 2 cut(s) 223, 504
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 4 cut(s) 79, 155, 199, 616
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 249, 627, 649
NlaIII CATG 6 cut(s) 148, 373, 494, 604, 609, 656
NlaIV GGNNCC 3 cut(s) 535, 590, 598
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 2 cut(s) 437, 493
NotI GCGGCCGC 1 cut(s) 50
NspI RCATGY 1 cut(s) 494
OliI CACNNNNGTG 1 cut(s) 525
PaeR7I CTCGAG 1 cut(s) 556
PasI CCCWGGG 1 cut(s) 222
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 623
PflMI CCANNNNNTGG 1 cut(s) 606
PkrI GCNGC 7 cut(s) 51, 54, 160, 219, 388, 421, 612
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PscI ACATGT 1 cut(s) 490
Psp6I CCWGG 2 cut(s) 221, 502
PspGI CCWGG 2 cut(s) 221, 502
PspN4I GGNNCC 3 cut(s) 535, 590, 598
PspPI GGNCC 2 cut(s) 219, 589
PspXI VCTCGAGB 1 cut(s) 556
PsuI RGATCY 1 cut(s) 249
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 2 cut(s) 120, 525
SacII CCGCGG 1 cut(s) 387
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 611
Sau3AI GATC 3 cut(s) 249, 627, 649
Sau96I GGNCC 2 cut(s) 219, 589
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 223, 261, 504
SfaNI GCATC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 556
Sfr303I CCGCGG 1 cut(s) 387
SgrBI CCGCGG 1 cut(s) 387
SinI GGWCC 1 cut(s) 589
SlaI CTCGAG 1 cut(s) 556
SmiMI CAYNNNNRTG 2 cut(s) 120, 525
SmlI CTYRAG 2 cut(s) 43, 556
SmoI CTYRAG 2 cut(s) 43, 556
Sse9I AATT 1 cut(s) 396
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 8 cut(s) 49, 53, 158, 217, 384, 386, 419, 422
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 3 cut(s) 221, 259, 502
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 39, 92, 476
TaqI TCGA 3 cut(s) 545, 557, 574
TasI AATT 1 cut(s) 396
TauI GCSGC 6 cut(s) 52, 55, 161, 220, 389, 422
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseFI GTSAC 2 cut(s) 437, 493
TseI GCWGC 1 cut(s) 610
Tsp45I GTSAC 2 cut(s) 437, 493
Van91I CCANNNNNTGG 1 cut(s) 606
VpaK11BI GGWCC 1 cut(s) 589
XceI RCATGY 1 cut(s) 494
XhoI CTCGAG 1 cut(s) 556
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.