MD17G1118900.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016899
Physical Location & Seq
Forward (+)
10293684 .. 10294349
666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1118900.v1.1.491

Sequence Viewer

Length: 666 bp
ATGTTCCATAAGTTTCTCAACGTCCATAAACTCAAACCGTTCTCACGGCGGCCGCAAGCCCCAGAAGCAGAGCCGGAGGGGGAGGAGGGGTGTACTTCTGTCTTGAAGATTGTCCATGTCGGCGGACGTGTTGAGAGGTACTACATGGCTACTCCGGCCGCCAAGATATTGGAGAAGCACCCCTCTTTCTTGCTGGCTAGGCCTGAGGTTTTTAGGCGGCCCTGGGATTCGGTAGTTAAGCCTGATGAGATCCTCACGCCCGGTGAGAAAATACTCCTTATTCCTCAACGAACCGTGAGGAAGCTCCGGAGTAGAATGAGAAAACCTAACAAAGAATTTCCCGTCAACCCTTTTGTATCTCAATCTTCCATGGATGTCTCAACCGCAGCCGAGACAATTTCGCGCTGGAAACAAGGTGCCGCGGCTGGTGAGCTACGTGACACTTCTGTTTGTGGTATTCTCAGAAGAAAGAAAAGTGTTGGCAAGAAACATGTGAGATTCAGTGGCATTGATGTCAAGCACAACGGTGGTGAAACCGAGCGCTCGAGTGAAAGCTTGAGCGCTCAATCTCATCAAGGGAAGAGAAGGGTACGAAGTGCTGTGACATGGCAACCGTCATTTACAGCAATCACAGAAACTCGTGGGAACGATCGTGTGAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

222

Amino Acids

25.01

Weight (kDa)

10.47

Isoelectric Point (pI)

65.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 31 - 212 6.1e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 416
AccII CGCG 2 cut(s) 403, 422
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 8 cut(s) 49, 53, 123, 159, 217, 384, 420, 422
AclWI GGATC 1 cut(s) 244
AcoI YGGCCR 2 cut(s) 50, 156
AcsI RAATTY 1 cut(s) 335
AfaI GTAC 3 cut(s) 94, 140, 591
AfeI AGCGCT 2 cut(s) 542, 562
AflIII ACRYGT 2 cut(s) 127, 490
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 1 cut(s) 221
AleI CACNNNNGTG 1 cut(s) 525
AluBI AGCT 3 cut(s) 304, 433, 555
AluI AGCT 3 cut(s) 304, 433, 555
Alw26I GTCTC 2 cut(s) 382, 386
AlwI GGATC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 544
Aor13HI TCCGGA 1 cut(s) 306
Aor51HI AGCGCT 2 cut(s) 542, 562
AoxI GGCC 4 cut(s) 50, 156, 200, 218
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 335
AspLEI GCGC 3 cut(s) 405, 543, 563
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 3 cut(s) 275, 440, 542
AvaI CYCGRG 1 cut(s) 544
AxyI CCTNAGG 1 cut(s) 204
BanI GGYRCC 1 cut(s) 416
BauI CACGAG 1 cut(s) 639
BbvI GCAGC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 223
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 382, 386
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 2 cut(s) 544, 564
BisI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 423
BlsI GCNGC 7 cut(s) 51, 54, 160, 219, 388, 421, 424
Bme1390I CCNGG 2 cut(s) 223, 261
BmeT110I CYCGRG 1 cut(s) 544
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 2 cut(s) 223, 261
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 437
BsaJI CCNNGG 4 cut(s) 221, 222, 369, 420
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse21I CCTNAGG 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 4 cut(s) 221, 222, 369, 420
BseGI GGATG 1 cut(s) 379
BseMII CTCAG 2 cut(s) 195, 475
BseRI GAGGAG 1 cut(s) 98
BseX3I CGGCCG 2 cut(s) 50, 156
BseXI GCAGC 1 cut(s) 398
Bsh1236I CGCG 2 cut(s) 403, 422
Bsh1285I CGRYCG 3 cut(s) 53, 159, 652
BshFI GGCC 4 cut(s) 52, 158, 202, 220
BshNI GGYRCC 1 cut(s) 416
BsiEI CGRYCG 3 cut(s) 53, 159, 652
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 4 cut(s) 74, 155, 261, 307
BsmAI GTCTC 2 cut(s) 382, 386
BsnI GGCC 4 cut(s) 52, 158, 202, 220
BsoBI CYCGRG 1 cut(s) 544
Bsp13I TCCGGA 1 cut(s) 306
Bsp143I GATC 2 cut(s) 249, 649
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 8 cut(s) 49, 53, 123, 159, 217, 384, 420, 422
BspANI GGCC 4 cut(s) 52, 158, 202, 220
BspCNI CTCAG 2 cut(s) 196, 474
BspEI TCCGGA 1 cut(s) 306
BspFNI CGCG 2 cut(s) 403, 422
BspLI GGNNCC 1 cut(s) 418
BspPI GGATC 1 cut(s) 244
BspT107I GGYRCC 1 cut(s) 416
BssECI CCNNGG 4 cut(s) 221, 222, 369, 420
BssMI GATC 2 cut(s) 249, 649
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 4 cut(s) 39, 295, 527, 615
Bst6I CTCTTC 1 cut(s) 575
BstBAI YACGTR 1 cut(s) 437
BstC8I GCNNGC 2 cut(s) 57, 195
BstDEI CTNAG 2 cut(s) 204, 461
BstDSI CCRYGG 2 cut(s) 369, 420
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 2 cut(s) 403, 422
BstH2I RGCGCY 2 cut(s) 544, 564
BstHHI GCGC 3 cut(s) 405, 543, 563
BstKTI GATC 2 cut(s) 252, 652
BstMAI GTCTC 2 cut(s) 382, 386
BstMBI GATC 2 cut(s) 249, 649
BstMCI CGRYCG 3 cut(s) 53, 159, 652
BstMWI GCNNNNNNNGC 3 cut(s) 65, 155, 199
BstNI CCWGG 1 cut(s) 223
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 221, 259
BstUI CGCG 2 cut(s) 403, 422
BstV1I GCAGC 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 249
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 249
BstZI CGGCCG 2 cut(s) 50, 156
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 4 cut(s) 52, 158, 202, 220
BtgI CCRYGG 2 cut(s) 369, 420
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 2 cut(s) 57, 195
CciNI GCGGCCGC 1 cut(s) 50
CfoI GCGC 3 cut(s) 405, 543, 563
Cfr13I GGNCC 1 cut(s) 219
Cfr42I CCGCGG 1 cut(s) 423
Csp6I GTAC 3 cut(s) 93, 139, 590
CviAII CATG 5 cut(s) 116, 145, 370, 491, 606
CviQI GTAC 3 cut(s) 93, 139, 590
DdeI CTNAG 2 cut(s) 204, 461
DpnI GATC 2 cut(s) 251, 651
DpnII GATC 2 cut(s) 249, 649
EaeI YGGCCR 2 cut(s) 50, 156
EagI CGGCCG 2 cut(s) 50, 156
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 138
EclXI CGGCCG 2 cut(s) 50, 156
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 202
Eco47III AGCGCT 2 cut(s) 542, 562
Eco52I CGGCCG 2 cut(s) 50, 156
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 119, 148, 373, 494, 609
FaiI YATR 7 cut(s) 9, 27, 117, 146, 371, 492, 607
FatI CATG 5 cut(s) 115, 144, 369, 490, 605
Fnu4HI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 423
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 423
FspBI CTAG 1 cut(s) 198
GlaI GCGC 3 cut(s) 404, 542, 562
GluI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 423
HaeII RGCGCY 2 cut(s) 544, 564
HaeIII GGCC 4 cut(s) 52, 158, 202, 220
HapII CCGG 4 cut(s) 74, 155, 261, 307
HhaI GCGC 3 cut(s) 405, 543, 563
Hin1II CATG 5 cut(s) 119, 148, 373, 494, 609
Hin6I GCGC 3 cut(s) 403, 541, 561
HinP1I GCGC 3 cut(s) 403, 541, 561
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 553
HinfI GANTC 2 cut(s) 227, 498
HpaII CCGG 4 cut(s) 74, 155, 261, 307
HphI GGTGA 3 cut(s) 275, 440, 542
Hpy166II GTNNAC 2 cut(s) 93, 346
Hpy188I TCNGA 1 cut(s) 464
Hpy188III TCNNGA 2 cut(s) 103, 307
Hpy8I GTNNAC 2 cut(s) 93, 346
HpyAV CCTTC 1 cut(s) 579
HpyCH4III ACNGT 4 cut(s) 39, 295, 527, 615
HpyCH4IV ACGT 3 cut(s) 21, 127, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 155, 199
HpyF3I CTNAG 2 cut(s) 204, 461
HpySE526I ACGT 3 cut(s) 21, 127, 436
Hsp92II CATG 5 cut(s) 119, 148, 373, 494, 609
HspAI GCGC 3 cut(s) 403, 541, 561
Kpn2I TCCGGA 1 cut(s) 306
KspI CCGCGG 1 cut(s) 423
Kzo9I GATC 2 cut(s) 249, 649
LmnI GCTCC 1 cut(s) 309
Lsp1109I GCAGC 1 cut(s) 398
MaeI CTAG 1 cut(s) 198
MaeII ACGT 3 cut(s) 21, 127, 436
MaeIII GTNAC 2 cut(s) 437, 601
MalI GATC 2 cut(s) 251, 651
MboI GATC 2 cut(s) 249, 649
MboII GAAGA 4 cut(s) 118, 357, 477, 592
MflI RGATCY 1 cut(s) 249
MluCI AATT 2 cut(s) 335, 396
MnlI CCTC 9 cut(s) 70, 76, 79, 129, 193, 199, 263, 291, 294
MroI TCCGGA 1 cut(s) 306
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 4 cut(s) 74, 155, 261, 307
MspR9I CCNGG 2 cut(s) 223, 261
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 2 cut(s) 403, 422
MwoI GCNNNNNNNGC 3 cut(s) 65, 155, 199
NciI CCSGG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 369
NdeII GATC 2 cut(s) 249, 649
NlaIII CATG 5 cut(s) 119, 148, 373, 494, 609
NlaIV GGNNCC 1 cut(s) 418
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 2 cut(s) 437, 601
NotI GCGGCCGC 1 cut(s) 50
NspI RCATGY 1 cut(s) 494
OliI CACNNNNGTG 1 cut(s) 525
PaeR7I CTCGAG 1 cut(s) 544
PasI CCCWGGG 1 cut(s) 222
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 1 cut(s) 490
PfeI GAWTC 2 cut(s) 227, 498
PkrI GCNGC 7 cut(s) 51, 54, 160, 219, 388, 421, 424
Ple19I CGATCG 1 cut(s) 652
Ppu21I YACGTR 1 cut(s) 437
PscI ACATGT 1 cut(s) 490
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 219
PspXI VCTCGAGB 1 cut(s) 544
PsuI RGATCY 1 cut(s) 249
PvuI CGATCG 1 cut(s) 652
RsaI GTAC 3 cut(s) 94, 140, 591
RsaNI GTAC 3 cut(s) 93, 139, 590
RseI CAYNNNNRTG 1 cut(s) 525
SacII CCGCGG 1 cut(s) 423
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 7 cut(s) 50, 53, 159, 218, 387, 420, 423
Sau3AI GATC 2 cut(s) 249, 649
Sau96I GGNCC 1 cut(s) 219
ScrFI CCNGG 2 cut(s) 223, 261
Sfr274I CTCGAG 1 cut(s) 544
Sfr303I CCGCGG 1 cut(s) 423
SgrBI CCGCGG 1 cut(s) 423
SlaI CTCGAG 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 2 cut(s) 544, 556
SmoI CTYRAG 2 cut(s) 544, 556
Sse9I AATT 2 cut(s) 335, 396
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 8 cut(s) 49, 53, 123, 159, 217, 384, 420, 422
SspMI CTAG 1 cut(s) 198
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 221, 259
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 39, 295, 527, 615
TaiI ACGT 3 cut(s) 24, 130, 439
TaqI TCGA 1 cut(s) 545
TasI AATT 2 cut(s) 335, 396
TatI WGTACW 1 cut(s) 92
TauI GCSGC 6 cut(s) 52, 55, 161, 220, 422, 425
TfiI GAWTC 2 cut(s) 227, 498
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 2 cut(s) 437, 601
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 2 cut(s) 437, 601
TspRI CASTG 1 cut(s) 508
XapI RAATTY 1 cut(s) 335
XceI RCATGY 1 cut(s) 494
XhoI CTCGAG 1 cut(s) 544
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.