Rw2G042330

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016899
Physical Location & Seq
Reverse (-)
68644728 .. 68645508
781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G042330.1

Sequence Viewer

Length: 762 bp
ATGTCCAGTACTTTGCAGAAGAACTATAGCCAAGTTGTCATGCTCCACAAATTCCTCAATATCGGTAAACTTAAGCCATTCTCAAGGCGTGTGCCTGTGCCGGTGCCGCCTAAGCAAGCTCCGGCACAGTCAGAGACGGGCAGCAACTCTATTTTGAAGGTTGTTCACGCAGGTGGAAATGTTGAGAGTTACTACATGGCTATTCCTGCAGCCAATATTTTGGAGAAGTACCCTTCTTTCTTGCTCGCTAGGCCTGAAGTCTTCAGGCGGCCGTGGGACTCAGTAGTGAAGCCGGATGCAATACTCACACCCGGAGAGAAAGTCTTCCTTGTTCCCCGCAGGACTGTGAGGAAGCTCCGTCGCAGAATACGAAGACCCGGGAAGGATTTTTCAGTCAATTGTTTCATGTCTCAATCGTCTATTGATGTATCAACTGAGACTCTACCACAGAGGAGAGATGTCGGTAGTGCAGTTGGTGATGCAAGTGAGAGTGACGCTTCTATTTGTAGTGCTATTAGCAGAAACAAAAGTGATGGCAAGAAGCATGTGACTTTTCGTGGCATCAAGAAACATGTGACATTTGCTGGCCTTGATGTGAAGCACAGGCCGGAGAAGACTCACAGCGAAATCGAGGAGCAAGATTCGAGTGAAAGCTCGAGTTCTCTAAAATCTCTCCGAGGGAGAAGGAGGGTACGGGTTGTGACATGGCAGCCGACTCTTACAGCAATAACAGAAGCTCATGGGAATGATTTGATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

253

Amino Acids

28.27

Weight (kDa)

10.27

Isoelectric Point (pI)

79.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 44 - 245 2.4e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 103
AciI CCGC 3 cut(s) 107, 268, 337
AcoI YGGCCR 1 cut(s) 269
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 2 cut(s) 247, 276
AfaI GTAC 3 cut(s) 10, 230, 693
AflII CTTAAG 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 1 cut(s) 157
AleI CACNNNNGTG 1 cut(s) 171
AluBI AGCT 4 cut(s) 119, 355, 654, 737
AluI AGCT 4 cut(s) 119, 355, 654, 737
Alw26I GTCTC 3 cut(s) 128, 414, 431
Ama87I CYCGRG 2 cut(s) 377, 655
AoxI GGCC 4 cut(s) 251, 269, 586, 605
ApeKI GCWGC 3 cut(s) 141, 209, 709
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 323
AsuC2I CCSGG 3 cut(s) 312, 378, 379
AsuHPI GGTGA 1 cut(s) 488
AvaI CYCGRG 2 cut(s) 377, 655
BanI GGYRCC 1 cut(s) 103
BbsI GAAGAC 4 cut(s) 253, 316, 379, 620
BbvI GCAGC 3 cut(s) 153, 221, 721
BccI CCATC 1 cut(s) 527
BceAI ACGGC 1 cut(s) 256
BcnI CCSGG 3 cut(s) 312, 378, 379
BcoDI GTCTC 3 cut(s) 128, 414, 431
BfaI CTAG 1 cut(s) 249
BfmI CTRYAG 2 cut(s) 25, 207
BfrI CTTAAG 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 161
BisI GCNGC 5 cut(s) 107, 142, 210, 269, 710
BlsI GCNGC 5 cut(s) 108, 143, 211, 270, 711
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 3 cut(s) 312, 378, 379
BmeT110I CYCGRG 2 cut(s) 377, 655
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 3 cut(s) 312, 378, 379
BmsI GCATC 3 cut(s) 286, 469, 570
BpiI GAAGAC 4 cut(s) 253, 316, 379, 620
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 67
BpuMI CCSGG 3 cut(s) 312, 378, 379
BsaJI CCNNGG 3 cut(s) 272, 377, 676
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 272, 377, 676
BseGI GGATG 1 cut(s) 301
BseMII CTCAG 2 cut(s) 294, 426
BseNI ACTGG 1 cut(s) 6
BseRI GAGGAG 2 cut(s) 466, 647
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 3 cut(s) 153, 221, 721
BsgI GTGCAG 1 cut(s) 489
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 4 cut(s) 253, 271, 588, 607
BshNI GGYRCC 1 cut(s) 103
BsiEI CGRYCG 1 cut(s) 272
BsiHKCI CYCGRG 2 cut(s) 377, 655
BsiSI CCGG 6 cut(s) 101, 122, 293, 312, 378, 608
BslFI GGGAC 1 cut(s) 290
BsmAI GTCTC 3 cut(s) 128, 414, 431
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 4 cut(s) 253, 271, 588, 607
BsoBI CYCGRG 2 cut(s) 377, 655
BspACI CCGC 3 cut(s) 107, 268, 337
BspANI GGCC 4 cut(s) 253, 271, 588, 607
BspCNI CTCAG 2 cut(s) 293, 427
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 1 cut(s) 6
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 272, 377, 676
Bst4CI ACNGT 2 cut(s) 129, 346
BstAFI CTTAAG 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 117, 246, 586
BstDEI CTNAG 3 cut(s) 111, 280, 435
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 301
BstMAI GTCTC 3 cut(s) 128, 414, 431
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 4 cut(s) 106, 112, 206, 250
BstNSI RCATGY 2 cut(s) 548, 575
BstSCI CCNGG 3 cut(s) 310, 376, 377
BstSFI CTRYAG 2 cut(s) 25, 207
BstV1I GCAGC 3 cut(s) 153, 221, 721
BstV2I GAAGAC 4 cut(s) 253, 316, 379, 620
BstXI CCANNNNNNTGG 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 269
BsuRI GGCC 4 cut(s) 253, 271, 588, 607
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 1 cut(s) 301
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 3 cut(s) 117, 246, 586
Cfr10I RCCGGY 1 cut(s) 100
Cfr9I CCCGGG 1 cut(s) 377
CseI GACGC 1 cut(s) 503
Csp6I GTAC 3 cut(s) 9, 229, 692
CviAII CATG 7 cut(s) 40, 196, 406, 545, 572, 705, 740
CviQI GTAC 3 cut(s) 9, 229, 692
DdeI CTNAG 3 cut(s) 111, 280, 435
EaeI YGGCCR 1 cut(s) 269
EagI CGGCCG 1 cut(s) 269
EclXI CGGCCG 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 253
Eco52I CGGCCG 1 cut(s) 269
Eco57I CTGAAG 2 cut(s) 247, 276
Eco88I CYCGRG 2 cut(s) 377, 655
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 7 cut(s) 43, 199, 409, 548, 575, 708, 743
FaiI YATR 8 cut(s) 27, 41, 197, 407, 546, 573, 706, 741
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 1 cut(s) 290
FatI CATG 7 cut(s) 39, 195, 405, 544, 571, 704, 739
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 5 cut(s) 107, 142, 210, 269, 710
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 5 cut(s) 107, 142, 210, 269, 710
FspBI CTAG 1 cut(s) 249
GluI GCNGC 5 cut(s) 107, 142, 210, 269, 710
HaeIII GGCC 4 cut(s) 253, 271, 588, 607
HapII CCGG 6 cut(s) 101, 122, 293, 312, 378, 608
HgaI GACGC 1 cut(s) 503
Hin1II CATG 7 cut(s) 43, 199, 409, 548, 575, 708, 743
HinfI GANTC 5 cut(s) 278, 439, 616, 641, 715
HpaII CCGG 6 cut(s) 101, 122, 293, 312, 378, 608
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 2 cut(s) 68, 166
Hpy188I TCNGA 2 cut(s) 133, 677
Hpy188III TCNNGA 1 cut(s) 565
Hpy8I GTNNAC 2 cut(s) 68, 166
Hpy99I CGWCG 1 cut(s) 363
HpyAV CCTTC 4 cut(s) 151, 243, 376, 678
HpyCH4III ACNGT 2 cut(s) 129, 346
HpyCH4V TGCA 5 cut(s) 16, 209, 299, 470, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 112, 206, 250
HpyF3I CTNAG 3 cut(s) 111, 280, 435
Hsp92II CATG 7 cut(s) 43, 199, 409, 548, 575, 708, 743
LmnI GCTCC 4 cut(s) 48, 124, 360, 634
Lsp1109I GCAGC 3 cut(s) 153, 221, 721
LweI GCATC 3 cut(s) 286, 469, 570
MaeI CTAG 1 cut(s) 249
MaeIII GTNAC 5 cut(s) 188, 491, 547, 574, 700
MboII GAAGA 5 cut(s) 31, 253, 316, 384, 625
MfeI CAATTG 1 cut(s) 397
MluCI AATT 2 cut(s) 50, 397
MlyI GAGTC 4 cut(s) 272, 433, 610, 709
MnlI CCTC 6 cut(s) 65, 342, 444, 625, 671, 681
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 1 cut(s) 72
MslI CAYNNNNRTG 2 cut(s) 171, 744
MspCI CTTAAG 1 cut(s) 71
MspI CCGG 6 cut(s) 101, 122, 293, 312, 378, 608
MspR9I CCNGG 3 cut(s) 312, 378, 379
MunI CAATTG 1 cut(s) 397
MwoI GCNNNNNNNGC 4 cut(s) 106, 112, 206, 250
NciI CCSGG 3 cut(s) 312, 378, 379
NlaIII CATG 7 cut(s) 43, 199, 409, 548, 575, 708, 743
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 4 cut(s) 491, 547, 574, 700
NspI RCATGY 2 cut(s) 548, 575
OliI CACNNNNGTG 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 655
PaqCI CACCTGC 1 cut(s) 161
PceI AGGCCT 1 cut(s) 253
PciI ACATGT 1 cut(s) 571
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 641
PkrI GCNGC 5 cut(s) 108, 143, 211, 270, 711
PleI GAGTC 4 cut(s) 272, 433, 610, 709
PpsI GAGTC 4 cut(s) 272, 433, 610, 709
PscI ACATGT 1 cut(s) 571
PspN4I GGNNCC 1 cut(s) 105
PspXI VCTCGAGB 1 cut(s) 655
PstI CTGCAG 1 cut(s) 211
RsaI GTAC 3 cut(s) 10, 230, 693
RsaNI GTAC 3 cut(s) 9, 229, 692
RseI CAYNNNNRTG 2 cut(s) 171, 744
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 5 cut(s) 107, 142, 210, 269, 710
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 4 cut(s) 272, 433, 610, 709
ScrFI CCNGG 3 cut(s) 312, 378, 379
SetI ASST 6 cut(s) 121, 162, 175, 357, 656, 739
SfaNI GCATC 3 cut(s) 286, 469, 570
SfcI CTRYAG 2 cut(s) 25, 207
Sfr274I CTCGAG 1 cut(s) 655
SlaI CTCGAG 1 cut(s) 655
SmaI CCCGGG 1 cut(s) 379
SmiMI CAYNNNNRTG 2 cut(s) 171, 744
SmlI CTYRAG 3 cut(s) 71, 82, 655
SmoI CTYRAG 3 cut(s) 71, 82, 655
Sse9I AATT 2 cut(s) 50, 397
SseBI AGGCCT 1 cut(s) 253
SsiI CCGC 3 cut(s) 107, 268, 337
SspI AATATT 1 cut(s) 217
SspMI CTAG 1 cut(s) 249
StuI AGGCCT 1 cut(s) 253
StyD4I CCNGG 3 cut(s) 310, 376, 377
TaaI ACNGT 2 cut(s) 129, 346
TaqI TCGA 3 cut(s) 630, 644, 656
TasI AATT 2 cut(s) 50, 397
TatI WGTACW 1 cut(s) 8
TauI GCSGC 2 cut(s) 109, 271
TfiI GAWTC 1 cut(s) 641
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseFI GTSAC 4 cut(s) 491, 547, 574, 700
TseI GCWGC 3 cut(s) 141, 209, 709
Tsp45I GTSAC 4 cut(s) 491, 547, 574, 700
TspDTI ATGAA 1 cut(s) 394
TspGWI ACGGA 1 cut(s) 347
TspMI CCCGGG 1 cut(s) 377
Vha464I CTTAAG 1 cut(s) 71
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 548, 575
XhoI CTCGAG 1 cut(s) 655
XmaI CCCGGG 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 249
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.