Rh2AG515100

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0016899
Physical Location & Seq
Reverse (-)
74250116 .. 74250961
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG515100.1

Sequence Viewer

Length: 846 bp
ATGCCCTTTTGCCTCTTAGTATGCATCGATGTGCGGAATGTTATAAAGGGTGCGTATTGTTTCAGCTCTGGACTGCTACGATTGATGTCCAGTACTTTGCAGAAGAACTATAGCCAAGTTGCCATGCTCCACAAGTTCCTCAATATCGGTAAACTCAAGCCATTCTCAAAGCGTGTGCCTGTGCCGGTGCCGCCTAAGCAAGCTCCGGCACAGTCAGAGACTGGCAGCAACTCTATTTTGAAGGTTGTTCACGCAGGTGGAAATGTTGAGAGTTACTACATGGCTATTCCTGCAGCCAAGATTTTGGAGAAGTACCCTTCTTTCTTGCTCGCTAGGCCTGAAGTCTTTAGGCGGCCGTGGGACTCAGTAGTGAAGCCGGATGCAATACTCACACCCGGAGAGAAAGTCTTTCTTGTTCCCCGCAGGACTGTGAGGAAGCTCCGTCGCAGAATACGAAGACCCGGGAAGGATTTTTCAGTCAATTCTTTCATGTCTCAATCGTCTATTGATGTATCAACTGAGACTCTACCACAGAGGAGAGATGTCGGTAGTGCAGTTGGTGATGCAAGTGAGAGTGACGCTTCTATTTGTAGTGCTATTAGCAGAAACAAAAGTGATGGCAAGAAGCATGTGACTTTTTGTGGCATCAAGAAACATGTGACATTTGCTGGCCTTGATGTGAAGCACAGGCCGGAGAAGACTCACAGCGAAATCGAGGAGCAAGATTCGAGTGAAATCTCGAGTTCTCTAAAATCTCTCGGAGGGAGAAGGAGGGTACGGGTTGTGACATGGCAGCCGACTCTTACAGCAATAATAGAAGCTCATGGGAATGATTTGATGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

281

Amino Acids

31.22

Weight (kDa)

10.01

Isoelectric Point (pI)

73.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 72 - 273 2.4e-15 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016899)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g40440
malus_domestica MD09G1129900.v1.1 MD17G1118900.v1.1
prunus_persica Prupe.3G195300_v2.0.a1
pyrus_communis pycom09g05300
rosa_chinensis RchiOBHm_Chr2g0155671
rosa_multiflora Rmu_ssc0000430.1_g000001
rosa_roxburghii Rroxscaffold_2G00093570
rosa_rugosa Rorug02G0449000
rosa_samantha Rh2AG515100 Rh2BG526200 Rh2CG500000 Rh2DG535000
rosa_wichuraiana Rw2G042330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AarI CACCTGC 1 cut(s) 245
Acc36I ACCTGC 1 cut(s) 245
AccB1I GGYRCC 1 cut(s) 187
AciI CCGC 4 cut(s) 34, 191, 352, 421
AcoI YGGCCR 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 3 cut(s) 94, 314, 777
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 1 cut(s) 241
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 4 cut(s) 66, 203, 439, 821
AluI AGCT 4 cut(s) 66, 203, 439, 821
Alw26I GTCTC 3 cut(s) 212, 498, 515
AlwNI CAGNNNCTG 1 cut(s) 221
Ama87I CYCGRG 2 cut(s) 461, 739
AoxI GGCC 4 cut(s) 335, 353, 670, 689
ApeKI GCWGC 3 cut(s) 225, 293, 793
AsuC2I CCSGG 3 cut(s) 396, 462, 463
AsuHPI GGTGA 1 cut(s) 572
AvaI CYCGRG 2 cut(s) 461, 739
BanI GGYRCC 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 463, 704
BbvI GCAGC 3 cut(s) 237, 305, 805
BccI CCATC 1 cut(s) 611
BceAI ACGGC 1 cut(s) 340
BcnI CCSGG 3 cut(s) 396, 462, 463
BcoDI GTCTC 3 cut(s) 212, 498, 515
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 109, 291
BfuAI ACCTGC 1 cut(s) 245
BisI GCNGC 5 cut(s) 191, 226, 294, 353, 794
BlsI GCNGC 5 cut(s) 192, 227, 295, 354, 795
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 3 cut(s) 396, 462, 463
BmeT110I CYCGRG 2 cut(s) 461, 739
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 3 cut(s) 396, 462, 463
BmsI GCATC 4 cut(s) 33, 370, 553, 654
BpiI GAAGAC 2 cut(s) 463, 704
Bpu10I CCTNAGC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 3 cut(s) 396, 462, 463
Bsa29I ATCGAT 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 356, 461
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 90, 226
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 356, 461
BseGI GGATG 1 cut(s) 385
BseMII CTCAG 2 cut(s) 378, 510
BseNI ACTGG 2 cut(s) 90, 226
BseRI GAGGAG 2 cut(s) 550, 731
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 3 cut(s) 237, 305, 805
BsgI GTGCAG 1 cut(s) 573
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 4 cut(s) 337, 355, 672, 691
BshNI GGYRCC 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 27
BsiEI CGRYCG 1 cut(s) 356
BsiHKCI CYCGRG 2 cut(s) 461, 739
BsiSI CCGG 6 cut(s) 185, 206, 377, 396, 462, 692
BslFI GGGAC 1 cut(s) 374
BsmAI GTCTC 3 cut(s) 212, 498, 515
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 4 cut(s) 337, 355, 672, 691
BsoBI CYCGRG 2 cut(s) 461, 739
BspACI CCGC 4 cut(s) 34, 191, 352, 421
BspANI GGCC 4 cut(s) 337, 355, 672, 691
BspCNI CTCAG 2 cut(s) 377, 511
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 2 cut(s) 90, 226
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 356, 461
Bst4CI ACNGT 2 cut(s) 213, 430
BstC8I GCNNGC 3 cut(s) 201, 330, 670
BstDEI CTNAG 4 cut(s) 16, 195, 364, 519
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 385
BstMAI GTCTC 3 cut(s) 212, 498, 515
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 4 cut(s) 190, 196, 290, 334
BstNSI RCATGY 2 cut(s) 632, 659
BstSCI CCNGG 3 cut(s) 394, 460, 461
BstSFI CTRYAG 2 cut(s) 109, 291
BstV1I GCAGC 3 cut(s) 237, 305, 805
BstV2I GAAGAC 2 cut(s) 463, 704
BstXI CCANNNNNNTGG 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 353
Bsu15I ATCGAT 1 cut(s) 27
BsuRI GGCC 4 cut(s) 337, 355, 672, 691
BsuTUI ATCGAT 1 cut(s) 27
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 1 cut(s) 385
BveI ACCTGC 1 cut(s) 245
Cac8I GCNNGC 3 cut(s) 201, 330, 670
CaiI CAGNNNCTG 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 184
Cfr9I CCCGGG 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 27
CseI GACGC 1 cut(s) 587
Csp6I GTAC 3 cut(s) 93, 313, 776
CviAII CATG 7 cut(s) 124, 280, 490, 629, 656, 789, 824
CviQI GTAC 3 cut(s) 93, 313, 776
DdeI CTNAG 4 cut(s) 16, 195, 364, 519
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
EclXI CGGCCG 1 cut(s) 353
Eco147I AGGCCT 1 cut(s) 337
Eco52I CGGCCG 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 2 cut(s) 461, 739
EcoT22I ATGCAT 1 cut(s) 26
FaeI CATG 7 cut(s) 127, 283, 493, 632, 659, 792, 827
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 1 cut(s) 374
FatI CATG 7 cut(s) 123, 279, 489, 628, 655, 788, 823
FauI CCCGC 1 cut(s) 428
Fnu4HI GCNGC 5 cut(s) 191, 226, 294, 353, 794
FokI GGATG 1 cut(s) 392
Fsp4HI GCNGC 5 cut(s) 191, 226, 294, 353, 794
FspBI CTAG 1 cut(s) 333
GluI GCNGC 5 cut(s) 191, 226, 294, 353, 794
HaeIII GGCC 4 cut(s) 337, 355, 672, 691
HapII CCGG 6 cut(s) 185, 206, 377, 396, 462, 692
HgaI GACGC 1 cut(s) 587
Hin1II CATG 7 cut(s) 127, 283, 493, 632, 659, 792, 827
HinfI GANTC 5 cut(s) 362, 523, 700, 725, 799
HpaII CCGG 6 cut(s) 185, 206, 377, 396, 462, 692
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 2 cut(s) 152, 250
Hpy188I TCNGA 2 cut(s) 217, 761
Hpy188III TCNNGA 3 cut(s) 69, 649, 739
Hpy8I GTNNAC 2 cut(s) 152, 250
Hpy99I CGWCG 1 cut(s) 447
HpyAV CCTTC 4 cut(s) 235, 327, 460, 762
HpyCH4III ACNGT 2 cut(s) 213, 430
HpyCH4V TGCA 6 cut(s) 24, 100, 293, 383, 554, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 190, 196, 290, 334
HpyF3I CTNAG 4 cut(s) 16, 195, 364, 519
Hsp92II CATG 7 cut(s) 127, 283, 493, 632, 659, 792, 827
LmnI GCTCC 4 cut(s) 132, 208, 444, 718
Lsp1109I GCAGC 3 cut(s) 237, 305, 805
LweI GCATC 4 cut(s) 33, 370, 553, 654
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 5 cut(s) 272, 575, 631, 658, 784
MboII GAAGA 3 cut(s) 115, 468, 709
MluCI AATT 1 cut(s) 481
MlyI GAGTC 4 cut(s) 356, 517, 694, 793
MnlI CCTC 7 cut(s) 23, 149, 426, 528, 709, 755, 765
Mph1103I ATGCAT 1 cut(s) 26
MslI CAYNNNNRTG 3 cut(s) 29, 255, 828
MspI CCGG 6 cut(s) 185, 206, 377, 396, 462, 692
MspR9I CCNGG 3 cut(s) 396, 462, 463
MwoI GCNNNNNNNGC 4 cut(s) 190, 196, 290, 334
NciI CCSGG 3 cut(s) 396, 462, 463
NlaIII CATG 7 cut(s) 127, 283, 493, 632, 659, 792, 827
NlaIV GGNNCC 1 cut(s) 189
NmuCI GTSAC 4 cut(s) 575, 631, 658, 784
NsiI ATGCAT 1 cut(s) 26
NspI RCATGY 2 cut(s) 632, 659
OliI CACNNNNGTG 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 739
PaqCI CACCTGC 1 cut(s) 245
PceI AGGCCT 1 cut(s) 337
PciI ACATGT 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 451
PfeI GAWTC 1 cut(s) 725
PkrI GCNGC 5 cut(s) 192, 227, 295, 354, 795
PleI GAGTC 4 cut(s) 356, 517, 694, 793
PpsI GAGTC 4 cut(s) 356, 517, 694, 793
PscI ACATGT 1 cut(s) 655
PsiI TTATAA 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 189
PstI CTGCAG 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 221
RsaI GTAC 3 cut(s) 94, 314, 777
RsaNI GTAC 3 cut(s) 93, 313, 776
RseI CAYNNNNRTG 3 cut(s) 29, 255, 828
SatI GCNGC 5 cut(s) 191, 226, 294, 353, 794
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 4 cut(s) 356, 517, 694, 793
ScrFI CCNGG 3 cut(s) 396, 462, 463
SetI ASST 6 cut(s) 68, 205, 246, 259, 441, 823
SfaNI GCATC 4 cut(s) 33, 370, 553, 654
SfcI CTRYAG 2 cut(s) 109, 291
Sfr274I CTCGAG 1 cut(s) 739
SlaI CTCGAG 1 cut(s) 739
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 3 cut(s) 29, 255, 828
SmlI CTYRAG 2 cut(s) 155, 739
SmoI CTYRAG 2 cut(s) 155, 739
Sse9I AATT 1 cut(s) 481
SseBI AGGCCT 1 cut(s) 337
SsiI CCGC 4 cut(s) 34, 191, 352, 421
SspMI CTAG 1 cut(s) 333
StuI AGGCCT 1 cut(s) 337
StyD4I CCNGG 3 cut(s) 394, 460, 461
TaaI ACNGT 2 cut(s) 213, 430
TaqI TCGA 4 cut(s) 27, 714, 728, 740
TasI AATT 1 cut(s) 481
TatI WGTACW 1 cut(s) 92
TauI GCSGC 2 cut(s) 193, 355
TfiI GAWTC 1 cut(s) 725
TseFI GTSAC 4 cut(s) 575, 631, 658, 784
TseI GCWGC 3 cut(s) 225, 293, 793
Tsp45I GTSAC 4 cut(s) 575, 631, 658, 784
TspDTI ATGAA 1 cut(s) 478
TspGWI ACGGA 1 cut(s) 431
TspMI CCCGGG 1 cut(s) 461
XceI RCATGY 2 cut(s) 632, 659
XhoI CTCGAG 1 cut(s) 739
XmaI CCCGGG 1 cut(s) 461
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.