FvH4_6g41771

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015269
Physical Location & Seq
Forward (+)
32745627 .. 32748984
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41771.t1

Sequence Viewer

Length: 1011 bp
ATGCAAATCTCTCTGACCATTTCACCCTCCAAAGCCCCTCTAATTTTCCCAACCCACCACCCAATTCCCCAAACCCCAAAACCCCAGAGAGCCTCACTCCCAACCCTCAACTCCGTCAAAGCCACGTCGCCTTATCTGTCTGCAGTGGGCCGGGCCATTGAGGACGACGAGTACCGGGCGGCCCGGAACCAGGTTCTCCGGAAAGGTGTGGACTTGGAGGGCTATTCCATTGAAGGGGTCTCTGTTGGGGGTCATGAGACTTGCATTATCATCCCTGAGTTTAAGTGTGCTTTCGACATCGGAAGGTGTCCCTCCAGAGCCATTCATCAGAATTTTGTGTTCATCACTCATGCCCATCTTGACCACATTGGTGGGTTGCCGATGTATGTAGCCAGCCGTGGTTTGTACAACTTAAAGCCGCCAACTGTGTTTGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTGATGGACATTCACAGGAGTATGGGACATGTGGAGCTGGATCTTGAGTTGGTTGCATTGGATGTGGATGAAACATATGAATTGCGGAACAACCTTGTGGTGCGGCCATTTAGGACTCATCATGCCATACCCAGTCAGGGTTATGTATTATACTCAGTTAGAAAGAAGCTGAAGAAGCAGTACATGCATCTGAAAGGGAAACAGATTGAGAAATTAAAGAAGTCTGGTACTGAGATTACAGACACTATACTGTCACCAGAGGTGGCCTTCACAGGGGATACAACATCAGAGTATATGCTGGATCCACGTAATGCTGATGCCCTGAGGGCAAAGATTCTTATAACTGAGGCAACTTTTCTGGATGAAGGATTCACTATTGAGGATGCACAACAACATGGTCATACTCATATAAATGAGATCATTGAAAATGCTCAGTGGATTAGAAATAAGGCTATTTTGTTAACCCATTTCTCCTCCCGCTACCATTTAGAGGTAATCACATATCTGAAAGGATATTCGTCAAGCCGTATCAAGGTTGCAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

337

Amino Acids

37.85

Weight (kDa)

7.77

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B_2 PF12706 111 - 312 1.8e-11 Beta-lactamase superfamily domain
Anti-Pycsar_Apyc1 PF23023 112 - 313 1.5e-07 Anti-Pycsar protein Apyc1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AccIII TCCGGA 1 cut(s) 198
AciI CCGC 5 cut(s) 179, 419, 550, 568, 943
AclWI GGATC 3 cut(s) 513, 761, 774
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 4 cut(s) 173, 407, 647, 694
AfiI CCNNNNNNNGG 6 cut(s) 190, 234, 602, 738, 955, 997
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 233, 890
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 1 cut(s) 189
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 3 cut(s) 466, 502, 634
AluI AGCT 3 cut(s) 466, 502, 634
Alw26I GTCTC 2 cut(s) 244, 251
AlwI GGATC 3 cut(s) 513, 761, 774
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 5 cut(s) 148, 153, 180, 569, 729
ApoI RAATTY 1 cut(s) 331
AspS9I GGNCC 3 cut(s) 148, 153, 181
AsuC2I CCSGG 3 cut(s) 152, 176, 184
AsuHPI GGTGA 2 cut(s) 15, 711
AxyI CCTNAGG 1 cut(s) 788
BamHI GGATCC 1 cut(s) 766
BarI GAAGNNNNNNTAC 2 cut(s) 629, 661
BccI CCATC 2 cut(s) 363, 463
BceAI ACGGC 2 cut(s) 381, 975
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 1 cut(s) 736
BcnI CCSGG 3 cut(s) 152, 176, 184
BcoDI GTCTC 2 cut(s) 244, 251
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 736
BglI GCCNNNNNGGC 1 cut(s) 791
BisI GCNGC 3 cut(s) 180, 419, 569
BlsI GCNGC 3 cut(s) 181, 420, 570
Bme1390I CCNGG 4 cut(s) 152, 176, 184, 191
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 3 cut(s) 148, 153, 181
BmiI GGNNCC 2 cut(s) 188, 768
BmrFI CCNGG 4 cut(s) 152, 176, 184, 191
BmrI ACTGGG 1 cut(s) 591
BmsI GCATC 3 cut(s) 661, 772, 838
BmuI ACTGGG 1 cut(s) 591
BplI GAGNNNNNCTC 2 cut(s) 81, 113
BpmI CTGGAG 1 cut(s) 298
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 3 cut(s) 152, 176, 184
BsaAI YACGTR 1 cut(s) 773
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 234, 602, 738, 955, 997
Bse1I ACTGG 1 cut(s) 597
Bse21I CCTNAGG 1 cut(s) 788
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 6 cut(s) 270, 460, 532, 538, 832, 853
BseLI CCNNNNNNNGG 6 cut(s) 190, 234, 602, 738, 955, 997
BseMII CTCAG 6 cut(s) 267, 633, 687, 779, 801, 911
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 928
BshFI GGCC 5 cut(s) 150, 155, 182, 571, 731
BsiSI CCGG 4 cut(s) 151, 175, 184, 199
BslFI GGGAC 2 cut(s) 294, 504
BslI CCNNNNNNNGG 6 cut(s) 190, 234, 602, 738, 955, 997
BsmAI GTCTC 2 cut(s) 244, 251
BsmFI GGGAC 2 cut(s) 294, 504
BsnI GGCC 5 cut(s) 150, 155, 182, 571, 731
Bso31I GGTCTC 1 cut(s) 244
Bsp13I TCCGGA 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 3 cut(s) 505, 766, 882
BspACI CCGC 5 cut(s) 179, 419, 550, 568, 943
BspANI GGCC 5 cut(s) 150, 155, 182, 571, 731
BspCNI CTCAG 6 cut(s) 268, 632, 688, 780, 802, 910
BspEI TCCGGA 1 cut(s) 198
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 188, 768
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 3 cut(s) 513, 761, 774
BspTNI GGTCTC 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 1 cut(s) 597
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 3 cut(s) 505, 766, 882
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 427, 717
BstAPI GCANNNNNTGC 1 cut(s) 649
BstAUI TGTACA 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 394
BstDEI CTNAG 6 cut(s) 276, 619, 696, 788, 810, 897
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 6 cut(s) 270, 460, 532, 538, 832, 853
BstKTI GATC 3 cut(s) 508, 769, 885
BstMAI GTCTC 2 cut(s) 244, 251
BstMBI GATC 3 cut(s) 505, 766, 882
BstMWI GCNNNNNNNGC 3 cut(s) 640, 649, 791
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 2 cut(s) 497, 652
BstSCI CCNGG 4 cut(s) 150, 174, 182, 189
BstSFI CTRYAG 1 cut(s) 141
BstX2I RGATCY 2 cut(s) 505, 766
BstXI CCANNNNNNTGG 1 cut(s) 371
BstYI RGATCY 2 cut(s) 505, 766
Bsu36I CCTNAGG 1 cut(s) 788
BsuI GTATCC 1 cut(s) 736
BsuRI GGCC 5 cut(s) 150, 155, 182, 571, 731
BtgI CCRYGG 1 cut(s) 397
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 6 cut(s) 270, 460, 532, 538, 832, 853
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 905
Cac8I GCNNGC 1 cut(s) 394
CciI TCATGA 1 cut(s) 253
Cfr13I GGNCC 3 cut(s) 148, 153, 181
CsiI ACCWGGT 1 cut(s) 189
Csp6I GTAC 4 cut(s) 172, 406, 646, 693
CviAII CATG 6 cut(s) 254, 350, 494, 587, 649, 860
CviQI GTAC 4 cut(s) 172, 406, 646, 693
DdeI CTNAG 6 cut(s) 276, 619, 696, 788, 810, 897
DpnI GATC 3 cut(s) 507, 768, 884
DpnII GATC 3 cut(s) 505, 766, 882
EaeI YGGCCR 1 cut(s) 569
Eco31I GGTCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 654
FaeI CATG 6 cut(s) 257, 353, 497, 590, 652, 863
FaqI GGGAC 2 cut(s) 294, 504
FatI CATG 6 cut(s) 253, 349, 493, 586, 648, 859
FauI CCCGC 1 cut(s) 950
FauNDI CATATG 1 cut(s) 541
Fnu4HI GCNGC 3 cut(s) 180, 419, 569
FokI GGATG 6 cut(s) 257, 467, 539, 545, 839, 860
Fsp4HI GCNGC 3 cut(s) 180, 419, 569
GluI GCNGC 3 cut(s) 180, 419, 569
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 5 cut(s) 150, 155, 182, 571, 731
HapII CCGG 4 cut(s) 151, 175, 184, 199
Hin1II CATG 6 cut(s) 257, 353, 497, 590, 652, 863
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 3 cut(s) 580, 799, 834
HpaI GTTAAC 1 cut(s) 927
HpaII CCGG 4 cut(s) 151, 175, 184, 199
HphI GGTGA 2 cut(s) 15, 711
Hpy166II GTNNAC 2 cut(s) 211, 927
Hpy188I TCNGA 6 cut(s) 15, 302, 330, 657, 754, 972
Hpy188III TCNNGA 6 cut(s) 199, 254, 315, 359, 509, 824
Hpy8I GTNNAC 2 cut(s) 211, 927
Hpy99I CGWCG 2 cut(s) 130, 170
HpyAV CCTTC 4 cut(s) 227, 297, 742, 824
HpyCH4III ACNGT 2 cut(s) 427, 717
HpyCH4IV ACGT 2 cut(s) 125, 772
HpyCH4V TGCA 7 cut(s) 4, 143, 264, 521, 652, 851, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 640, 649, 791
HpyF3I CTNAG 6 cut(s) 276, 619, 696, 788, 810, 897
HpySE526I ACGT 2 cut(s) 125, 772
Hsp92II CATG 6 cut(s) 257, 353, 497, 590, 652, 863
Kpn2I TCCGGA 1 cut(s) 198
KspAI GTTAAC 1 cut(s) 927
Kzo9I GATC 3 cut(s) 505, 766, 882
LmnI GCTCC 1 cut(s) 499
LweI GCATC 3 cut(s) 661, 772, 838
MabI ACCWGGT 1 cut(s) 189
MaeII ACGT 2 cut(s) 125, 772
MaeIII GTNAC 1 cut(s) 717
MalI GATC 3 cut(s) 507, 768, 884
MboI GATC 3 cut(s) 505, 766, 882
MboII GAAGA 1 cut(s) 649
MflI RGATCY 2 cut(s) 505, 766
MluCI AATT 5 cut(s) 42, 63, 331, 545, 677
MlyI GAGTC 1 cut(s) 574
Mph1103I ATGCAT 1 cut(s) 654
MroI TCCGGA 1 cut(s) 198
MseI TTAA 5 cut(s) 282, 413, 447, 680, 926
MslI CAYNNNNRTG 2 cut(s) 369, 876
MspI CCGG 4 cut(s) 151, 175, 184, 199
MspR9I CCNGG 4 cut(s) 152, 176, 184, 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 640, 649, 791
NciI CCSGG 3 cut(s) 152, 176, 184
NdeI CATATG 1 cut(s) 541
NdeII GATC 3 cut(s) 505, 766, 882
NlaIII CATG 6 cut(s) 257, 353, 497, 590, 652, 863
NlaIV GGNNCC 2 cut(s) 188, 768
NmuCI GTSAC 1 cut(s) 717
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 2 cut(s) 497, 652
OliI CACNNNNGTG 1 cut(s) 369
PagI TCATGA 1 cut(s) 253
PciI ACATGT 1 cut(s) 493
PfeI GAWTC 2 cut(s) 799, 834
PkrI GCNGC 3 cut(s) 181, 420, 570
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 773
PscI ACATGT 1 cut(s) 493
PsiI TTATAA 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 188, 768
PspPI GGNCC 3 cut(s) 148, 153, 181
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 505, 766
RsaI GTAC 4 cut(s) 173, 407, 647, 694
RsaNI GTAC 4 cut(s) 172, 406, 646, 693
RseI CAYNNNNRTG 2 cut(s) 369, 876
SaqAI TTAA 5 cut(s) 282, 413, 447, 680, 926
SatI GCNGC 3 cut(s) 180, 419, 569
Sau3AI GATC 3 cut(s) 505, 766, 882
Sau96I GGNCC 3 cut(s) 148, 153, 181
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 4 cut(s) 152, 176, 184, 191
SexAI ACCWGGT 1 cut(s) 189
SfaNI GCATC 3 cut(s) 661, 772, 838
SfcI CTRYAG 1 cut(s) 141
SmiMI CAYNNNNRTG 2 cut(s) 369, 876
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 5 cut(s) 42, 63, 331, 545, 677
SsiI CCGC 5 cut(s) 179, 419, 550, 568, 943
StyD4I CCNGG 4 cut(s) 150, 174, 182, 189
TaaI ACNGT 2 cut(s) 427, 717
TaiI ACGT 2 cut(s) 128, 775
TaqI TCGA 1 cut(s) 294
TasI AATT 5 cut(s) 42, 63, 331, 545, 677
TatI WGTACW 2 cut(s) 405, 645
TauI GCSGC 3 cut(s) 182, 421, 571
TfiI GAWTC 2 cut(s) 799, 834
Tru1I TTAA 5 cut(s) 282, 413, 447, 680, 926
Tru9I TTAA 5 cut(s) 282, 413, 447, 680, 926
TscAI CASTG 2 cut(s) 150, 905
TseFI GTSAC 1 cut(s) 717
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 5 cut(s) 314, 331, 549, 558, 843
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 2 cut(s) 150, 905
XapI RAATTY 1 cut(s) 331
XceI RCATGY 2 cut(s) 497, 652
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.