Rroxscaffold_2G00092120

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015269
Physical Location & Seq
Reverse (-)
13659990 .. 13663008
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092120.1

Sequence Viewer

Length: 1095 bp
ATGCAAATCTCTCTGACCATTACACCCTCCAAAGCCCCTCTAATTTTCCCACTCCACCACCCCATTTCCCAAACCCCAAAACCCCACACAACTTCCCTTCAAACCCTCTCCAATCCTCTCAATTCCCTCAAAGCCACCTCGCCTTATCTATCTGCAGTGGGCCGGGCTATCGAGGACGACGAGTACCGGATAGCCCGGAGCCAGGTTCTCCGAAAAGGTGTGGACTTGGAGGGCTATTCCATTGAGGGGGTCTCCGTTGGGGGTCATGAGACTTGCATTATTATACCTGAGTTTAAGTGTGCTTTCGATATCGGAAGGTGTCCCTCCAGAGCCATTCATCAGAATTTTCTCTTCATCACTCATGCCCATCTTGACCACATTGGTGGGCTGCCGATGTATATAGCCAGCCGTGGTTTGTACAACTTAAAGCCTCCGACTGTGTTTGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTGATGGACATTCACAGGAGCATGGGACATGTGGAGCTGGAACTTGAATTGGTTGCATTGGATGTGGGTGAAACATATGAGCTGCGGAATAACCTTGTTGTCCGGCCATTTAGGACTCAGCATGCCATACCCAGCCAGGGTTATGTTATTTACTCGGTTAGAAAAAAACTGAAGAAGCAGTACATGCATCTGAAAGGGAAACAGATTGAGAAATTGAAAAAGTCTGGTACTGAGACACTATATTGTCACCAGAGGTGGCCTTCACAGGATACAACATCGGAGTATATGCTGGATCCACGTAATGCTGATGCCTTGAGGGCAAAGATTCTTATAACTGAGGCAACTTTTCTGGATGAAGGATTCAGTATTGAGGATGCACGACAACATGGTCATACACATATAAATGAGGCATGTGAACTATTGGTTTTCATGTTCCTTCTGAATACAGTGCATATCATTGAAAATGCTCAGTGGATTAGAAATAAGGCAATTTTGTTGACCCATTTCTCCTCCCGCTACCATTTAGAGGACATTCGTCAAGCTGTATCAAGGTTGCAGTCTAAGGTGTCAGCGAAAGTGGTCCCTCTTACAGAAGGTTTCAAATCCATGCACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

364

Amino Acids

41.33

Weight (kDa)

8.61

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B_2 PF12706 115 - 210 8.6e-07 Beta-lactamase superfamily domain
Anti-Pycsar_Apyc1 PF23023 116 - 206 1.3e-06 Anti-Pycsar protein Apyc1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
AasI GACNNNNNNGTC 1 cut(s) 864
AciI CCGC 2 cut(s) 562, 991
AclWI GGATC 2 cut(s) 764, 777
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 4 cut(s) 185, 419, 659, 706
AfiI CCNNNNNNNGG 4 cut(s) 202, 246, 614, 1003
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 5 cut(s) 101, 524, 694, 938, 1078
AjnI CCWGG 2 cut(s) 201, 612
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 4 cut(s) 478, 514, 559, 1019
AluI AGCT 4 cut(s) 478, 514, 559, 1019
Alw26I GTCTC 3 cut(s) 256, 263, 704
AlwI GGATC 2 cut(s) 764, 777
AoxI GGCC 3 cut(s) 160, 581, 734
ApeKI GCWGC 2 cut(s) 388, 559
ApoI RAATTY 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 160, 1057
AsuC2I CCSGG 2 cut(s) 164, 196
AsuHPI GGTGA 2 cut(s) 557, 716
AvaII GGWCC 1 cut(s) 1057
BamHI GGATCC 1 cut(s) 769
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbvI GCAGC 2 cut(s) 375, 546
BccI CCATC 2 cut(s) 375, 475
BceAI ACGGC 1 cut(s) 393
BciT130I CCWGG 2 cut(s) 203, 614
BciVI GTATCC 1 cut(s) 739
BcnI CCSGG 2 cut(s) 164, 196
BcoDI GTCTC 3 cut(s) 256, 263, 704
BfmI CTRYAG 1 cut(s) 153
BfuI GTATCC 1 cut(s) 739
BglI GCCNNNNNGGC 1 cut(s) 794
BisI GCNGC 2 cut(s) 389, 560
BlsI GCNGC 2 cut(s) 390, 561
Bme1390I CCNGG 4 cut(s) 164, 196, 203, 614
Bme18I GGWCC 1 cut(s) 1057
BmgT120I GGNCC 2 cut(s) 160, 1057
BmiI GGNNCC 3 cut(s) 200, 771, 1059
BmrFI CCNGG 4 cut(s) 164, 196, 203, 614
BmsI GCATC 3 cut(s) 673, 775, 841
BoxI GACNNNNGTC 1 cut(s) 1011
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 1 cut(s) 310
BpuEI CTTGAG 1 cut(s) 811
BpuMI CCSGG 2 cut(s) 164, 196
BsaAI YACGTR 1 cut(s) 776
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 409, 613
BsaWI WCCGGW 1 cut(s) 186
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 246, 614, 1003
BseBI CCWGG 2 cut(s) 203, 614
BseDI CCNNGG 2 cut(s) 409, 613
BseGI GGATG 4 cut(s) 472, 544, 835, 856
BseLI CCNNNNNNNGG 4 cut(s) 202, 246, 614, 1003
BseMII CTCAG 5 cut(s) 279, 608, 699, 804, 959
BseRI GAGGAG 1 cut(s) 976
BseXI GCAGC 2 cut(s) 375, 546
BseYI CCCAGC 1 cut(s) 608
BshFI GGCC 3 cut(s) 162, 583, 736
BsiSI CCGG 4 cut(s) 163, 187, 196, 580
BslFI GGGAC 3 cut(s) 306, 516, 1043
BslI CCNNNNNNNGG 4 cut(s) 202, 246, 614, 1003
BsmAI GTCTC 3 cut(s) 256, 263, 704
BsmFI GGGAC 3 cut(s) 306, 516, 1043
BsnI GGCC 3 cut(s) 162, 583, 736
Bso31I GGTCTC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 1 cut(s) 769
BspACI CCGC 2 cut(s) 562, 991
BspANI GGCC 3 cut(s) 162, 583, 736
BspCNI CTCAG 5 cut(s) 280, 607, 700, 805, 958
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 200, 771, 1059
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 2 cut(s) 764, 777
BspTNI GGTCTC 1 cut(s) 256
BsrGI TGTACA 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 409, 613
BssMI GATC 1 cut(s) 769
Bst2UI CCWGG 2 cut(s) 203, 614
Bst4CI ACNGT 2 cut(s) 439, 925
Bst6I CTCTTC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 776
BstC8I GCNNGC 2 cut(s) 406, 600
BstDEI CTNAG 7 cut(s) 288, 594, 708, 813, 945, 1038, 1092
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 4 cut(s) 472, 544, 835, 856
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 3 cut(s) 256, 263, 704
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 2 cut(s) 661, 794
BstNI CCWGG 2 cut(s) 203, 614
BstNSI RCATGY 4 cut(s) 509, 602, 664, 891
BstPAI GACNNNNGTC 1 cut(s) 1011
BstSCI CCNGG 4 cut(s) 162, 194, 201, 612
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 375, 546
BstX2I RGATCY 1 cut(s) 769
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 769
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 3 cut(s) 162, 583, 736
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 4 cut(s) 472, 544, 835, 856
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 3 cut(s) 162, 930, 953
Cac8I GCNNGC 2 cut(s) 406, 600
CciI TCATGA 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 160, 1057
Csp6I GTAC 4 cut(s) 184, 418, 658, 705
CviQI GTAC 4 cut(s) 184, 418, 658, 705
DdeI CTNAG 7 cut(s) 288, 594, 708, 813, 945, 1038, 1092
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
DrdI GACNNNNNNGTC 1 cut(s) 864
DseDI GACNNNNNNGTC 1 cut(s) 864
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 1057
Eco57I CTGAAG 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 201, 612
EcoRV GATATC 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 666
FaqI GGGAC 3 cut(s) 306, 516, 1043
FauI CCCGC 1 cut(s) 998
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 389, 560
FokI GGATG 4 cut(s) 479, 551, 842, 863
Fsp4HI GCNGC 2 cut(s) 389, 560
GluI GCNGC 2 cut(s) 389, 560
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 3 cut(s) 162, 583, 736
HapII CCGG 4 cut(s) 163, 187, 196, 580
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HinfI GANTC 3 cut(s) 592, 802, 837
HpaII CCGG 4 cut(s) 163, 187, 196, 580
HphI GGTGA 2 cut(s) 557, 716
Hpy166II GTNNAC 3 cut(s) 223, 893, 975
Hpy188I TCNGA 8 cut(s) 15, 212, 314, 342, 435, 669, 757, 918
Hpy188III TCNNGA 4 cut(s) 266, 327, 371, 827
Hpy8I GTNNAC 3 cut(s) 223, 893, 975
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 6 cut(s) 107, 309, 747, 827, 923, 1064
HpyCH4III ACNGT 2 cut(s) 439, 925
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 9 cut(s) 4, 155, 276, 533, 664, 854, 928, 1033, 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 661, 794
HpyF3I CTNAG 7 cut(s) 288, 594, 708, 813, 945, 1038, 1092
HpySE526I ACGT 1 cut(s) 775
Kzo9I GATC 1 cut(s) 769
LmnI GCTCC 3 cut(s) 198, 495, 511
Lsp1109I GCAGC 2 cut(s) 375, 546
LweI GCATC 3 cut(s) 673, 775, 841
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 1 cut(s) 722
MalI GATC 1 cut(s) 771
MboI GATC 1 cut(s) 769
MboII GAAGA 2 cut(s) 343, 661
MflI RGATCY 1 cut(s) 769
MluCI AATT 6 cut(s) 42, 121, 343, 524, 689, 966
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 458
Mph1103I ATGCAT 1 cut(s) 666
MseI TTAA 3 cut(s) 294, 425, 459
MslI CAYNNNNRTG 2 cut(s) 381, 879
MspI CCGG 4 cut(s) 163, 187, 196, 580
MspR9I CCNGG 4 cut(s) 164, 196, 203, 614
MvaI CCWGG 2 cut(s) 203, 614
MwoI GCNNNNNNNGC 2 cut(s) 661, 794
NciI CCSGG 2 cut(s) 164, 196
NdeI CATATG 1 cut(s) 553
NdeII GATC 1 cut(s) 769
NlaIV GGNNCC 3 cut(s) 200, 771, 1059
NmuCI GTSAC 1 cut(s) 722
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 4 cut(s) 509, 602, 664, 891
OliI CACNNNNGTG 1 cut(s) 381
PaeI GCATGC 1 cut(s) 602
PagI TCATGA 1 cut(s) 265
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 2 cut(s) 802, 837
PkrI GCNGC 2 cut(s) 390, 561
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 776
PscI ACATGT 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 1011
PsiI TTATAA 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 201, 612
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 201, 612
PspN4I GGNNCC 3 cut(s) 200, 771, 1059
PspPI GGNCC 2 cut(s) 160, 1057
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 769
RsaI GTAC 4 cut(s) 185, 419, 659, 706
RsaNI GTAC 4 cut(s) 184, 418, 658, 705
RseI CAYNNNNRTG 2 cut(s) 381, 879
SaqAI TTAA 3 cut(s) 294, 425, 459
SatI GCNGC 2 cut(s) 389, 560
Sau3AI GATC 1 cut(s) 769
Sau96I GGNCC 2 cut(s) 160, 1057
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 4 cut(s) 164, 196, 203, 614
SfaNI GCATC 3 cut(s) 673, 775, 841
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 1057
SmiMI CAYNNNNRTG 2 cut(s) 381, 879
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SphI GCATGC 1 cut(s) 602
Sse9I AATT 6 cut(s) 42, 121, 343, 524, 689, 966
SsiI CCGC 2 cut(s) 562, 991
StyD4I CCNGG 4 cut(s) 162, 194, 201, 612
TaaI ACNGT 2 cut(s) 439, 925
TaiI ACGT 1 cut(s) 778
TaqI TCGA 2 cut(s) 171, 306
TasI AATT 6 cut(s) 42, 121, 343, 524, 689, 966
TatI WGTACW 2 cut(s) 417, 657
TfiI GAWTC 2 cut(s) 802, 837
Tru1I TTAA 3 cut(s) 294, 425, 459
Tru9I TTAA 3 cut(s) 294, 425, 459
TscAI CASTG 3 cut(s) 162, 930, 953
TseFI GTSAC 1 cut(s) 722
TseI GCWGC 2 cut(s) 388, 559
Tsp45I GTSAC 1 cut(s) 722
TspDTI ATGAA 4 cut(s) 326, 343, 846, 895
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 3 cut(s) 162, 930, 953
VpaK11BI GGWCC 1 cut(s) 1057
XapI RAATTY 1 cut(s) 343
XceI RCATGY 4 cut(s) 509, 602, 664, 891
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.