Rh2CG510900

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015269
Physical Location & Seq
Forward (+)
68456688 .. 68459395
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG510900.1

Sequence Viewer

Length: 1059 bp
ATGCAAATCTCTCTGACCATTACACCCTCCAAAGCCCCTCTAATTTTCCCACTCCACCACCCCATTTCCCAAACCCCAAAACCCCACACAACTTCCCTTCAAACCCTCTCCAATCCTCTCAATTCCCTCAAAGCCACCTCGCCTTATCTATCTGCAGTGGGCCGGGCTATCGAGGACGACGAGTACCGGATTTCCCGGAACCAGGTTCTCCGAAAAGGTGTGGACTTGGAGGGCTACTCCATTGAGGGGGTCTCCGTTGGGGGTCATGAGACTTGTATCATTATACCGGAGTTTAAGTGTGCTTTCGATATCGGCAGGTGTCCCTCCAGAGCCATTCATCAGAATTTTCTCTTCATCACTCATGCCCATCTTGACCACATTGGTGGGCTGCCGATGTACATAGCCAGCCGTGGTTTGTACAACTTAAAGCCTCCGACTGTGTTTGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTGATGGACATTCACAGGAGCATGGGACATGTGGAGCTGGAACTTGAATTGGTTGCATTGGATGTGGGTGAAACATATGAGCTGCGGAATAATCTTGTTGTCCGGCCATTTAGGACTCAGCATGCCATACCCAGCCAGGGTTATGTTATTTACTCGGTTAGAAAAAAACTGAAGAAGCAGTACATGCATCTGAAAGGGAAACAGATTGAGAAATTGAAAAAGTCCGGTACTGAGATTACAGACACTATATTGTCACCAGAGGTGGCCTTCACAGGGGATACAACATCGGAGTATATGCTGGATCCACGTAATGCTGATGCCTTGAGGGCAAAGATTCTCATAACTGAGGCAACTTTTCTGGATGAAGGATTCAGTATTGAGGATGCACGACAACATGGTCATACACATATAAATGAGATCATTGAAAATGCTCAGTGGATTAGAAATAAGGCAATTTTGTTGACCCATTTCTCCTCCCGCTACCATTTAGAGGACATTCGTCAAGCTGTATCAAGGTTGCAGTCTAAGGTGTCAGCGAAAGTGGTCCCTCTTACAGAAGGTTTCAAATCCATGCACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

352

Amino Acids

39.69

Weight (kDa)

8.24

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B_2 PF12706 115 - 316 1.1e-10 Beta-lactamase superfamily domain
Anti-Pycsar_Apyc1 PF23023 116 - 320 1.3e-07 Anti-Pycsar protein Apyc1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 306
AasI GACNNNNNNGTC 1 cut(s) 873
Acc36I ACCTGC 1 cut(s) 306
AciI CCGC 2 cut(s) 562, 955
AclWI GGATC 2 cut(s) 773, 786
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 5 cut(s) 185, 398, 419, 659, 706
AfiI CCNNNNNNNGG 5 cut(s) 202, 246, 614, 750, 967
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 5 cut(s) 101, 524, 694, 902, 1042
AjnI CCWGG 2 cut(s) 201, 612
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 4 cut(s) 478, 514, 559, 983
AluI AGCT 4 cut(s) 478, 514, 559, 983
Alw26I GTCTC 2 cut(s) 256, 263
AlwI GGATC 2 cut(s) 773, 786
AoxI GGCC 3 cut(s) 160, 581, 741
ApeKI GCWGC 2 cut(s) 388, 559
ApoI RAATTY 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 160, 1021
AsuC2I CCSGG 2 cut(s) 164, 196
AsuHPI GGTGA 2 cut(s) 557, 723
AvaII GGWCC 1 cut(s) 1021
BamHI GGATCC 1 cut(s) 778
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbvI GCAGC 2 cut(s) 375, 546
BccI CCATC 2 cut(s) 375, 475
BceAI ACGGC 1 cut(s) 393
BciT130I CCWGG 2 cut(s) 203, 614
BciVI GTATCC 1 cut(s) 748
BcnI CCSGG 2 cut(s) 164, 196
BcoDI GTCTC 2 cut(s) 256, 263
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 306
BfuI GTATCC 1 cut(s) 748
BglI GCCNNNNNGGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 389, 560
BlsI GCNGC 2 cut(s) 390, 561
Bme1390I CCNGG 4 cut(s) 164, 196, 203, 614
Bme18I GGWCC 1 cut(s) 1021
BmgT120I GGNCC 2 cut(s) 160, 1021
BmiI GGNNCC 3 cut(s) 200, 780, 1023
BmrFI CCNGG 4 cut(s) 164, 196, 203, 614
BmsI GCATC 3 cut(s) 673, 784, 850
BoxI GACNNNNGTC 1 cut(s) 975
BplI GAGNNNNNCTC 4 cut(s) 221, 253, 236, 268
BpmI CTGGAG 1 cut(s) 310
BpuEI CTTGAG 1 cut(s) 820
BpuMI CCSGG 2 cut(s) 164, 196
BsaAI YACGTR 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 2 cut(s) 409, 613
BsaWI WCCGGW 3 cut(s) 186, 286, 701
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 246, 614, 750, 967
BseBI CCWGG 2 cut(s) 203, 614
BseDI CCNNGG 2 cut(s) 409, 613
BseGI GGATG 4 cut(s) 472, 544, 844, 865
BseLI CCNNNNNNNGG 5 cut(s) 202, 246, 614, 750, 967
BseMII CTCAG 4 cut(s) 608, 699, 813, 923
BseRI GAGGAG 1 cut(s) 940
BseXI GCAGC 2 cut(s) 375, 546
BseYI CCCAGC 1 cut(s) 608
BshFI GGCC 3 cut(s) 162, 583, 743
BsiSI CCGG 6 cut(s) 163, 187, 196, 287, 580, 702
BslFI GGGAC 3 cut(s) 306, 516, 1007
BslI CCNNNNNNNGG 5 cut(s) 202, 246, 614, 750, 967
BsmAI GTCTC 2 cut(s) 256, 263
BsmFI GGGAC 3 cut(s) 306, 516, 1007
BsnI GGCC 3 cut(s) 162, 583, 743
Bso31I GGTCTC 1 cut(s) 256
Bsp1407I TGTACA 2 cut(s) 396, 417
Bsp143I GATC 2 cut(s) 778, 894
BspACI CCGC 2 cut(s) 562, 955
BspANI GGCC 3 cut(s) 162, 583, 743
BspCNI CTCAG 4 cut(s) 607, 700, 814, 922
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 200, 780, 1023
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 306
BspPI GGATC 2 cut(s) 773, 786
BspTNI GGTCTC 1 cut(s) 256
BsrGI TGTACA 2 cut(s) 396, 417
BssECI CCNNGG 2 cut(s) 409, 613
BssMI GATC 2 cut(s) 778, 894
Bst2UI CCWGG 2 cut(s) 203, 614
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 356
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 2 cut(s) 396, 417
BstBAI YACGTR 1 cut(s) 785
BstC8I GCNNGC 2 cut(s) 406, 600
BstDEI CTNAG 6 cut(s) 594, 708, 822, 909, 1002, 1056
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 4 cut(s) 472, 544, 844, 865
BstKTI GATC 2 cut(s) 781, 897
BstMAI GTCTC 2 cut(s) 256, 263
BstMBI GATC 2 cut(s) 778, 894
BstMWI GCNNNNNNNGC 2 cut(s) 661, 803
BstNI CCWGG 2 cut(s) 203, 614
BstNSI RCATGY 3 cut(s) 509, 602, 664
BstPAI GACNNNNGTC 1 cut(s) 975
BstSCI CCNGG 4 cut(s) 162, 194, 201, 612
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 375, 546
BstX2I RGATCY 1 cut(s) 778
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 778
BsuI GTATCC 1 cut(s) 748
BsuRI GGCC 3 cut(s) 162, 583, 743
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 4 cut(s) 472, 544, 844, 865
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 917
BveI ACCTGC 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 406, 600
CciI TCATGA 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 160, 1021
CsiI ACCWGGT 1 cut(s) 201
Csp6I GTAC 5 cut(s) 184, 397, 418, 658, 705
CviAII CATG 8 cut(s) 266, 362, 499, 506, 599, 661, 872, 1048
CviQI GTAC 5 cut(s) 184, 397, 418, 658, 705
DdeI CTNAG 6 cut(s) 594, 708, 822, 909, 1002, 1056
DpnI GATC 2 cut(s) 780, 896
DpnII GATC 2 cut(s) 778, 894
DrdI GACNNNNNNGTC 1 cut(s) 873
DseDI GACNNNNNNGTC 1 cut(s) 873
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 201, 612
EcoRV GATATC 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 666
FaeI CATG 8 cut(s) 269, 365, 502, 509, 602, 664, 875, 1051
FaqI GGGAC 3 cut(s) 306, 516, 1007
FatI CATG 8 cut(s) 265, 361, 498, 505, 598, 660, 871, 1047
FauI CCCGC 1 cut(s) 962
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 389, 560
FokI GGATG 4 cut(s) 479, 551, 851, 872
Fsp4HI GCNGC 2 cut(s) 389, 560
GluI GCNGC 2 cut(s) 389, 560
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 3 cut(s) 162, 583, 743
HapII CCGG 6 cut(s) 163, 187, 196, 287, 580, 702
Hin1II CATG 8 cut(s) 269, 365, 502, 509, 602, 664, 875, 1051
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HinfI GANTC 3 cut(s) 592, 811, 846
HpaII CCGG 6 cut(s) 163, 187, 196, 287, 580, 702
HphI GGTGA 2 cut(s) 557, 723
Hpy166II GTNNAC 2 cut(s) 223, 939
Hpy188I TCNGA 6 cut(s) 15, 212, 342, 435, 669, 766
Hpy188III TCNNGA 4 cut(s) 266, 327, 371, 836
Hpy8I GTNNAC 2 cut(s) 223, 939
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 4 cut(s) 107, 754, 836, 1028
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 7 cut(s) 4, 155, 533, 664, 863, 997, 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 661, 803
HpyF3I CTNAG 6 cut(s) 594, 708, 822, 909, 1002, 1056
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 8 cut(s) 269, 365, 502, 509, 602, 664, 875, 1051
Kzo9I GATC 2 cut(s) 778, 894
LmnI GCTCC 2 cut(s) 495, 511
Lsp1109I GCAGC 2 cut(s) 375, 546
LweI GCATC 3 cut(s) 673, 784, 850
MabI ACCWGGT 1 cut(s) 201
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 1 cut(s) 729
MalI GATC 2 cut(s) 780, 896
MboI GATC 2 cut(s) 778, 894
MboII GAAGA 2 cut(s) 343, 661
MflI RGATCY 1 cut(s) 778
MluCI AATT 6 cut(s) 42, 121, 343, 524, 689, 930
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 458
Mph1103I ATGCAT 1 cut(s) 666
MseI TTAA 3 cut(s) 294, 425, 459
MslI CAYNNNNRTG 2 cut(s) 381, 888
MspI CCGG 6 cut(s) 163, 187, 196, 287, 580, 702
MspR9I CCNGG 4 cut(s) 164, 196, 203, 614
MvaI CCWGG 2 cut(s) 203, 614
MwoI GCNNNNNNNGC 2 cut(s) 661, 803
NciI CCSGG 2 cut(s) 164, 196
NdeI CATATG 1 cut(s) 553
NdeII GATC 2 cut(s) 778, 894
NlaIII CATG 8 cut(s) 269, 365, 502, 509, 602, 664, 875, 1051
NlaIV GGNNCC 3 cut(s) 200, 780, 1023
NmuCI GTSAC 1 cut(s) 729
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 3 cut(s) 509, 602, 664
OliI CACNNNNGTG 1 cut(s) 381
PaeI GCATGC 1 cut(s) 602
PagI TCATGA 1 cut(s) 265
PaqCI CACCTGC 1 cut(s) 306
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 2 cut(s) 811, 846
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 2 cut(s) 390, 561
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Ppu21I YACGTR 1 cut(s) 785
PscI ACATGT 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 201, 612
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 2 cut(s) 201, 612
PspN4I GGNNCC 3 cut(s) 200, 780, 1023
PspPI GGNCC 2 cut(s) 160, 1021
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 5 cut(s) 185, 398, 419, 659, 706
RsaNI GTAC 5 cut(s) 184, 397, 418, 658, 705
RseI CAYNNNNRTG 2 cut(s) 381, 888
SaqAI TTAA 3 cut(s) 294, 425, 459
SatI GCNGC 2 cut(s) 389, 560
Sau3AI GATC 2 cut(s) 778, 894
Sau96I GGNCC 2 cut(s) 160, 1021
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 4 cut(s) 164, 196, 203, 614
SexAI ACCWGGT 1 cut(s) 201
SfaNI GCATC 3 cut(s) 673, 784, 850
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 1021
SmiMI CAYNNNNRTG 2 cut(s) 381, 888
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
SphI GCATGC 1 cut(s) 602
Sse9I AATT 6 cut(s) 42, 121, 343, 524, 689, 930
SsiI CCGC 2 cut(s) 562, 955
StyD4I CCNGG 4 cut(s) 162, 194, 201, 612
TaaI ACNGT 1 cut(s) 439
TaiI ACGT 1 cut(s) 787
TaqI TCGA 2 cut(s) 171, 306
TasI AATT 6 cut(s) 42, 121, 343, 524, 689, 930
TatI WGTACW 3 cut(s) 396, 417, 657
TfiI GAWTC 2 cut(s) 811, 846
Tru1I TTAA 3 cut(s) 294, 425, 459
Tru9I TTAA 3 cut(s) 294, 425, 459
TscAI CASTG 2 cut(s) 162, 917
TseFI GTSAC 1 cut(s) 729
TseI GCWGC 2 cut(s) 388, 559
Tsp45I GTSAC 1 cut(s) 729
TspDTI ATGAA 3 cut(s) 326, 343, 855
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 2 cut(s) 162, 917
VpaK11BI GGWCC 1 cut(s) 1021
XapI RAATTY 1 cut(s) 343
XceI RCATGY 3 cut(s) 509, 602, 664
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.