MD09G1117300.v1.1

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015269
Physical Location & Seq
Reverse (-)
9009483 .. 9012967
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1117300.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGCAAATTTCTCTGACCATTTCACCCTCCAAAGCCCCTCTAATTTTCCCCTTCCACCAACCCATCTCCCAAATACCAAAGCCCCAGAGAGCTTCACTGCAAACCCATGCCAATTCCGTAAATTCCCTCAAAGGGTCGCCTTTACTTCCCGCATTCGGTCGGGCAATCGAGGACGAAGAGTACCGGCTGGCCCGGTCCCAGGTTCTCCGGAAAGGTATGGACTTGGAGGGCTTGAACATTGAGGGTCTCTCGATTGGCGGCCATGAGACCTGCATTATCATCCCGGAGTTCAAGTGCGCTTTCGACATCGGGAGGTGCCCCTCCAGAGCCATTCATCAGAACAATTTGTTCATCACTCATGCCCATCTTGATCATATTGGTGGGCTGCCGATGTATGTAGCCAGCCGTGGTTTGTACAACTTGAAACCTCCAACGGTATTTGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTAATAGACATTCACAGAACGATGGGTCATGTGGAATTGAATCTTGATTTGGTTGCATTGGATGTTGGTGAAACATATGAATTGCGGAATAACCTTGTTGTCCGGCCATTTAGGACTCAACATGCTATACCTAGCCAGGGTTATGTGATTTACTCGGTTAGAAAAAAGCTGAAGAAGCAGTACATTCATCTAAAAGGGAAGCAGATTGAGAAATTGAAGAAGTCTGGTGTTGAGATTACAGACGTTATATTGTCACCAGAAGTGGCCTTCACAGGGGATACAACGTCAGATTATATGCTGGATCCACGCAATGCTGATGCCTTGAGGGCAAAGATTCTTATAACTGAGGCAACTTTTCTGGATGAAGGGTGCAGCATTGAGCATGCACGACAACATGGTCATACTCATATAGACGAGATCATTGAGAATGCCCAATGGATAAGAAATAAGTCAGTTCTGTTAACCCATTTCTCCTCCCGCTACCATCTAGAGGACATTCGTCAAGCTGTATCAAAGTTGCAGTCCAAGCTGTCAGCAAAAGTGATCCCTCTTACAGAAGGTTTCAAATCTATCCACGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

352

Amino Acids

39.43

Weight (kDa)

8.67

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anti-Pycsar_Apyc1 PF23023 114 - 319 8.2e-09 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 116 - 315 1.7e-10 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AasI GACNNNNNNGTC 1 cut(s) 870
Acc36I ACCTGC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 207
AciI CCGC 4 cut(s) 150, 258, 559, 952
AclWI GGATC 3 cut(s) 770, 783, 1012
AcoI YGGCCR 2 cut(s) 259, 578
AcsI RAATTY 2 cut(s) 6, 121
AcuI CTGAAG 1 cut(s) 665
AfaI GTAC 3 cut(s) 182, 416, 656
AfiI CCNNNNNNNGG 6 cut(s) 132, 155, 199, 611, 747, 964
AgsI TTSAA 6 cut(s) 235, 292, 424, 514, 691, 1039
AjnI CCWGG 2 cut(s) 198, 609
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 5 cut(s) 92, 475, 643, 980, 1003
AluI AGCT 5 cut(s) 92, 475, 643, 980, 1003
Alw26I GTCTC 2 cut(s) 251, 260
AlwI GGATC 3 cut(s) 770, 783, 1012
Aor13HI TCCGGA 1 cut(s) 207
AoxI GGCC 4 cut(s) 189, 259, 578, 738
ApeKI GCWGC 2 cut(s) 385, 846
ApoI RAATTY 2 cut(s) 6, 121
ArsI GACNNNNNNTTYG 2 cut(s) 980, 1012
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 190, 195
AsuC2I CCSGG 2 cut(s) 193, 284
AsuHPI GGTGA 3 cut(s) 15, 554, 720
AvaII GGWCC 1 cut(s) 195
BaeGI GKGCMC 1 cut(s) 320
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 315
BarI GAAGNNNNNNTAC 2 cut(s) 638, 670
BbvI GCAGC 2 cut(s) 372, 858
BccI CCATC 4 cut(s) 71, 372, 490, 966
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 2 cut(s) 200, 611
BciVI GTATCC 1 cut(s) 745
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 2 cut(s) 193, 284
BcoDI GTCTC 2 cut(s) 251, 260
BfaI CTAG 2 cut(s) 606, 962
BfuAI ACCTGC 1 cut(s) 278
BfuI GTATCC 1 cut(s) 745
BglI GCCNNNNNGGC 1 cut(s) 800
BisI GCNGC 3 cut(s) 259, 386, 847
BlsI GCNGC 3 cut(s) 260, 387, 848
Bme1390I CCNGG 4 cut(s) 193, 200, 284, 611
Bme18I GGWCC 1 cut(s) 195
BmgT120I GGNCC 2 cut(s) 190, 195
BmiI GGNNCC 3 cut(s) 197, 317, 777
BmrFI CCNGG 4 cut(s) 193, 200, 284, 611
BmsI GCATC 1 cut(s) 781
BoxI GACNNNNGTC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 1 cut(s) 307
BpuEI CTTGAG 1 cut(s) 817
BpuMI CCSGG 2 cut(s) 193, 284
BsaI GGTCTC 2 cut(s) 251, 260
BsaJI CCNNGG 3 cut(s) 198, 406, 610
BsaWI WCCGGW 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 6 cut(s) 132, 155, 199, 611, 747, 964
Bse118I RCCGGY 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 790
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 2 cut(s) 200, 611
BseDI CCNNGG 3 cut(s) 198, 406, 610
BseGI GGATG 4 cut(s) 279, 469, 541, 841
BseLI CCNNNNNNNGG 6 cut(s) 132, 155, 199, 611, 747, 964
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 1 cut(s) 810
BseRI GAGGAG 1 cut(s) 937
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 2 cut(s) 372, 858
BsgI GTGCAG 1 cut(s) 865
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 4 cut(s) 191, 261, 580, 740
BshNI GGYRCC 1 cut(s) 315
BsiEI CGRYCG 1 cut(s) 160
BsiSI CCGG 5 cut(s) 184, 193, 208, 284, 577
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 6 cut(s) 132, 155, 199, 611, 747, 964
BsmAI GTCTC 2 cut(s) 251, 260
BsmFI GGGAC 1 cut(s) 181
BsmI GAATGC 2 cut(s) 152, 907
BsnI GGCC 4 cut(s) 191, 261, 580, 740
Bso31I GGTCTC 2 cut(s) 251, 260
Bsp1286I GDGCHC 1 cut(s) 320
Bsp13I TCCGGA 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 4 cut(s) 370, 775, 891, 1017
BspACI CCGC 4 cut(s) 150, 258, 559, 952
BspANI GGCC 4 cut(s) 191, 261, 580, 740
BspCNI CTCAG 1 cut(s) 811
BspEI TCCGGA 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 197, 317, 777
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 3 cut(s) 770, 783, 1012
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 251, 260
BsrDI GCAATG 1 cut(s) 790
BsrFI RCCGGY 1 cut(s) 183
BsrGI TGTACA 1 cut(s) 414
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 3 cut(s) 198, 406, 610
BssMI GATC 4 cut(s) 370, 775, 891, 1017
Bst2UI CCWGG 2 cut(s) 200, 611
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 189, 403, 858
BstDEI CTNAG 2 cut(s) 819, 1053
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 4 cut(s) 279, 469, 541, 841
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 4 cut(s) 373, 778, 894, 1020
BstMAI GTCTC 2 cut(s) 251, 260
BstMBI GATC 4 cut(s) 370, 775, 891, 1017
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 649, 800, 1000
BstNI CCWGG 2 cut(s) 200, 611
BstNSI RCATGY 2 cut(s) 599, 860
BstPAI GACNNNNGTC 1 cut(s) 972
BstSCI CCNGG 4 cut(s) 191, 198, 282, 609
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 372, 858
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 4 cut(s) 191, 261, 580, 740
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 4 cut(s) 279, 469, 541, 841
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 3 cut(s) 189, 403, 858
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 190, 195
Csp6I GTAC 3 cut(s) 181, 415, 655
CviAII CATG 7 cut(s) 107, 263, 359, 503, 596, 857, 869
CviQI GTAC 3 cut(s) 181, 415, 655
DdeI CTNAG 2 cut(s) 819, 1053
DpnI GATC 4 cut(s) 372, 777, 893, 1019
DpnII GATC 4 cut(s) 370, 775, 891, 1017
DrdI GACNNNNNNGTC 1 cut(s) 870
DseDI GACNNNNNNGTC 1 cut(s) 870
EaeI YGGCCR 2 cut(s) 259, 578
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco31I GGTCTC 2 cut(s) 251, 260
Eco47I GGWCC 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 198, 609
FaeI CATG 7 cut(s) 110, 266, 362, 506, 599, 860, 872
FaqI GGGAC 1 cut(s) 181
FatI CATG 7 cut(s) 106, 262, 358, 502, 595, 856, 868
FauI CCCGC 2 cut(s) 157, 959
FauNDI CATATG 1 cut(s) 550
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 259, 386, 847
FokI GGATG 4 cut(s) 266, 476, 548, 848
Fsp4HI GCNGC 3 cut(s) 259, 386, 847
FspBI CTAG 2 cut(s) 606, 962
GlaI GCGC 1 cut(s) 298
GluI GCNGC 3 cut(s) 259, 386, 847
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 4 cut(s) 191, 261, 580, 740
HapII CCGG 5 cut(s) 184, 193, 208, 284, 577
HhaI GCGC 1 cut(s) 299
Hin1II CATG 7 cut(s) 110, 266, 362, 506, 599, 860, 872
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HinfI GANTC 3 cut(s) 514, 589, 808
HpaI GTTAAC 1 cut(s) 936
HpaII CCGG 5 cut(s) 184, 193, 208, 284, 577
HphI GGTGA 3 cut(s) 15, 554, 720
Hpy166II GTNNAC 1 cut(s) 936
Hpy188I TCNGA 3 cut(s) 15, 339, 763
Hpy188III TCNNGA 8 cut(s) 208, 250, 310, 324, 368, 518, 833, 962
Hpy8I GTNNAC 1 cut(s) 936
HpyAV CCTTC 4 cut(s) 61, 751, 833, 1025
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 717, 758
HpyCH4V TGCA 7 cut(s) 4, 100, 273, 530, 846, 860, 994
HpyF10VI GCNNNNNNNGC 3 cut(s) 649, 800, 1000
HpyF3I CTNAG 2 cut(s) 819, 1053
HpySE526I ACGT 2 cut(s) 717, 758
Hsp92II CATG 7 cut(s) 110, 266, 362, 506, 599, 860, 872
HspAI GCGC 1 cut(s) 297
Kpn2I TCCGGA 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 370
KspAI GTTAAC 1 cut(s) 936
Kzo9I GATC 4 cut(s) 370, 775, 891, 1017
Lsp1109I GCAGC 2 cut(s) 372, 858
LweI GCATC 1 cut(s) 781
MaeI CTAG 2 cut(s) 606, 962
MaeII ACGT 2 cut(s) 717, 758
MaeIII GTNAC 1 cut(s) 726
MalI GATC 4 cut(s) 372, 777, 893, 1019
MboI GATC 4 cut(s) 370, 775, 891, 1017
MboII GAAGA 3 cut(s) 188, 658, 703
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 8 cut(s) 6, 42, 112, 121, 343, 509, 554, 686
MlyI GAGTC 1 cut(s) 583
MmeI TCCRAC 1 cut(s) 455
MroI TCCGGA 1 cut(s) 207
MseI TTAA 2 cut(s) 456, 935
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 5 cut(s) 184, 193, 208, 284, 577
MspR9I CCNGG 4 cut(s) 193, 200, 284, 611
Mva1269I GAATGC 2 cut(s) 152, 907
MvaI CCWGG 2 cut(s) 200, 611
MwoI GCNNNNNNNGC 3 cut(s) 649, 800, 1000
NciI CCSGG 2 cut(s) 193, 284
NdeI CATATG 1 cut(s) 550
NdeII GATC 4 cut(s) 370, 775, 891, 1017
NlaIII CATG 7 cut(s) 110, 266, 362, 506, 599, 860, 872
NlaIV GGNNCC 3 cut(s) 197, 317, 777
NmuCI GTSAC 1 cut(s) 726
NspI RCATGY 2 cut(s) 599, 860
PaeI GCATGC 1 cut(s) 860
PctI GAATGC 2 cut(s) 152, 907
PfeI GAWTC 2 cut(s) 514, 808
PfoI TCCNGGA 1 cut(s) 282
PkrI GCNGC 3 cut(s) 260, 387, 848
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
PshAI GACNNNNGTC 1 cut(s) 972
PsiI TTATAA 1 cut(s) 815
Psp6I CCWGG 2 cut(s) 198, 609
PspGI CCWGG 2 cut(s) 198, 609
PspN4I GGNNCC 3 cut(s) 197, 317, 777
PspPI GGNCC 2 cut(s) 190, 195
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 3 cut(s) 182, 416, 656
RsaNI GTAC 3 cut(s) 181, 415, 655
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 2 cut(s) 456, 935
SatI GCNGC 3 cut(s) 259, 386, 847
Sau3AI GATC 4 cut(s) 370, 775, 891, 1017
Sau96I GGNCC 2 cut(s) 190, 195
SchI GAGTC 1 cut(s) 583
ScrFI CCNGG 4 cut(s) 193, 200, 284, 611
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 1 cut(s) 781
SinI GGWCC 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
SphI GCATGC 1 cut(s) 860
Sse9I AATT 8 cut(s) 6, 42, 112, 121, 343, 509, 554, 686
SsiI CCGC 4 cut(s) 150, 258, 559, 952
SspMI CTAG 2 cut(s) 606, 962
StyD4I CCNGG 4 cut(s) 191, 198, 282, 609
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 720, 761
TaqI TCGA 3 cut(s) 168, 251, 303
TaqII GACCGA 1 cut(s) 146
TasI AATT 8 cut(s) 6, 42, 112, 121, 343, 509, 554, 686
TatI WGTACW 2 cut(s) 414, 654
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 514, 808
Tru1I TTAA 2 cut(s) 456, 935
Tru9I TTAA 2 cut(s) 456, 935
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 2 cut(s) 385, 846
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 5 cut(s) 323, 340, 567, 650, 852
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 195
XapI RAATTY 2 cut(s) 6, 121
XbaI TCTAGA 1 cut(s) 961
XceI RCATGY 2 cut(s) 599, 860
XspI CTAG 2 cut(s) 606, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.