pycom09g04240

Ribonuclease z

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015269
Physical Location & Seq
Reverse (-)
3094174 .. 3097119
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04240.7

Sequence Viewer

Length: 795 bp
ATGAGACCTGCATTATCGTCCCGGAGTTCAAATGCGCTTTCGACATCGGGAGGTGCCCCTCCAGGGCCATTCATCAGAACTTTTTGTTCATCACTCATGCCCATCTTGATCGATATTGGTGGGCTGCCGATGTATGTAGCTAGTCGTGGTTTGTACAACTTGAAACCTCCAACGGTATTTGTGCCTCCTTGTATTAAAGAGGATGTGGAGAAGCTAATAGACATTCACAGAACGATGGGTCATGTGGAATTGAATCTTGATTTGGTTGCATTGGATGTTGGTGAAACATATGAATTGCGGAATAACCTTGTTGTCCGGCCATTTAGGACTCAACATGCTATACCTAGCCAGGGTTATGTGATTTACTCAGTTAGAAAAAAGCTGAAGAAGCAGTACATTCATCTAAAAGGGAAGCAGATTGAGAAATTGAAGAAGTCTGGTGTTGAGATTACAGACGTTATATTGTCACCAGAAGTAGCCTTCACAGGGGATACAACGTCAGATTATATGCTGGATCCACGCAATGCTGATGCCTTGAGGGCAAAGATTCTTATAACTGAGGCAACTTTTCTGGATGAAGGGTGCAGCATTGAGCATGCACGGCAACATGGTCATACACATATAGACGAGATCATTGAGAACGCCCAATGGATAAGAAATAAGTCAGTTCTGTTAACCCATTTTTCCTCCCGCTACCATCTGGAGGACATTCGTCAAGCTGTATCAAGGTTGCAGTCCAAGCTGTCAGCAAAAGTGATCCCTCTTACAGAAGGTTTCAAATCTATCCACGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Lactamase_B_2

Protein Analysis

265

Amino Acids

29.55

Weight (kDa)

9.03

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04530
fragaria_vesca FvH4_6g41771 FvH4_6g41771 FvH4_6g41771 FvH4_6g41771
malus_domestica MD09G1117300.v1.1
prunus_persica Prupe.3G206500_v2.0.a1
pyrus_communis pycom09g04240
rosa_chinensis RchiOBHm_Chr2g0157561
rosa_laevigata RLG00000020998
rosa_roxburghii Rroxscaffold_2G00092120
rosa_rugosa Rorug02G0462100
rosa_samantha Rh2AG526200 Rh2BG540900 Rh2CG510900 Rh2DG549400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 2 cut(s) 298, 691
AclWI GGATC 3 cut(s) 509, 522, 751
AcoI YGGCCR 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 2 cut(s) 155, 395
AfiI CCNNNNNNNGG 4 cut(s) 63, 350, 486, 703
AgsI TTSAA 5 cut(s) 30, 163, 253, 430, 778
AjnI CCWGG 2 cut(s) 61, 348
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 5 cut(s) 140, 214, 382, 719, 742
AluI AGCT 5 cut(s) 140, 214, 382, 719, 742
AlwI GGATC 3 cut(s) 509, 522, 751
AoxI GGCC 2 cut(s) 65, 317
ApeKI GCWGC 2 cut(s) 124, 585
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 65
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 2 cut(s) 293, 459
BaeGI GKGCMC 1 cut(s) 58
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 53
BarI GAAGNNNNNNTAC 2 cut(s) 377, 409
BbvI GCAGC 2 cut(s) 111, 597
BccI CCATC 3 cut(s) 110, 229, 705
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 2 cut(s) 63, 350
BciVI GTATCC 1 cut(s) 484
BcnI CCSGG 1 cut(s) 22
BfaI CTAG 2 cut(s) 141, 345
BfuAI ACCTGC 1 cut(s) 16
BfuI GTATCC 1 cut(s) 484
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 2 cut(s) 125, 586
BlsI GCNGC 2 cut(s) 126, 587
Bme1390I CCNGG 3 cut(s) 22, 63, 350
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 2 cut(s) 55, 516
BmrFI CCNGG 3 cut(s) 22, 63, 350
BmsI GCATC 1 cut(s) 520
BoxI GACNNNNGTC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 45, 722
BpuEI CTTGAG 1 cut(s) 556
BpuMI CCSGG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 62, 349
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 350, 486, 703
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 2 cut(s) 63, 350
BseCI ATCGAT 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 62, 349
BseGI GGATG 3 cut(s) 208, 280, 580
BseLI CCNNNNNNNGG 4 cut(s) 63, 350, 486, 703
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 381, 549
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 2 cut(s) 111, 597
BsgI GTGCAG 1 cut(s) 604
BshFI GGCC 2 cut(s) 67, 319
BshNI GGYRCC 1 cut(s) 53
BshVI ATCGAT 1 cut(s) 111
BsiSI CCGG 2 cut(s) 22, 316
BslFI GGGAC 1 cut(s) 4
BslI CCNNNNNNNGG 4 cut(s) 63, 350, 486, 703
BsmFI GGGAC 1 cut(s) 4
BsnI GGCC 2 cut(s) 67, 319
Bsp1286I GDGCHC 1 cut(s) 58
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 4 cut(s) 108, 514, 630, 756
BspACI CCGC 2 cut(s) 298, 691
BspANI GGCC 2 cut(s) 67, 319
BspCNI CTCAG 2 cut(s) 380, 550
BspDI ATCGAT 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 55, 516
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 3 cut(s) 509, 522, 751
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 529
BsrGI TGTACA 1 cut(s) 153
BssECI CCNNGG 2 cut(s) 62, 349
BssMI GATC 4 cut(s) 108, 514, 630, 756
Bst2UI CCWGG 2 cut(s) 63, 350
Bst4CI ACNGT 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 367, 558, 792
BstF5I GGATG 3 cut(s) 208, 280, 580
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 4 cut(s) 111, 517, 633, 759
BstMBI GATC 4 cut(s) 108, 514, 630, 756
BstMWI GCNNNNNNNGC 4 cut(s) 388, 539, 601, 739
BstNI CCWGG 2 cut(s) 63, 350
BstNSI RCATGY 2 cut(s) 338, 599
BstPAI GACNNNNGTC 1 cut(s) 711
BstSCI CCNGG 3 cut(s) 20, 61, 348
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 111, 597
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
Bsu15I ATCGAT 1 cut(s) 111
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 2 cut(s) 67, 319
BsuTUI ATCGAT 1 cut(s) 111
BtsCI GGATG 3 cut(s) 208, 280, 580
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 597
CfoI GCGC 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 65
ClaI ATCGAT 1 cut(s) 111
Csp6I GTAC 2 cut(s) 154, 394
CviAII CATG 5 cut(s) 97, 242, 335, 596, 608
CviQI GTAC 2 cut(s) 154, 394
DdeI CTNAG 3 cut(s) 367, 558, 792
DpnI GATC 4 cut(s) 110, 516, 632, 758
DpnII GATC 4 cut(s) 108, 514, 630, 756
EaeI YGGCCR 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 61, 348
FaeI CATG 5 cut(s) 100, 245, 338, 599, 611
FaqI GGGAC 1 cut(s) 4
FatI CATG 5 cut(s) 96, 241, 334, 595, 607
FauI CCCGC 1 cut(s) 698
FauNDI CATATG 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 125, 586
FokI GGATG 3 cut(s) 215, 287, 587
Fsp4HI GCNGC 2 cut(s) 125, 586
FspBI CTAG 2 cut(s) 141, 345
GlaI GCGC 1 cut(s) 36
GluI GCNGC 2 cut(s) 125, 586
GsuI CTGGAG 2 cut(s) 45, 722
HaeIII GGCC 2 cut(s) 67, 319
HapII CCGG 2 cut(s) 22, 316
HhaI GCGC 1 cut(s) 37
Hin1II CATG 5 cut(s) 100, 245, 338, 599, 611
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 3 cut(s) 253, 328, 547
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 2 cut(s) 22, 316
HphI GGTGA 2 cut(s) 293, 459
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 2 cut(s) 77, 502
Hpy188III TCNNGA 5 cut(s) 48, 106, 257, 572, 701
Hpy8I GTNNAC 1 cut(s) 675
HpyAV CCTTC 3 cut(s) 490, 572, 764
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 2 cut(s) 456, 497
HpyCH4V TGCA 5 cut(s) 11, 269, 585, 599, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 539, 601, 739
HpyF3I CTNAG 3 cut(s) 367, 558, 792
HpySE526I ACGT 2 cut(s) 456, 497
Hsp92II CATG 5 cut(s) 100, 245, 338, 599, 611
HspAI GCGC 1 cut(s) 35
KspAI GTTAAC 1 cut(s) 675
Kzo9I GATC 4 cut(s) 108, 514, 630, 756
Lsp1109I GCAGC 2 cut(s) 111, 597
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 141, 345
MaeII ACGT 2 cut(s) 456, 497
MaeIII GTNAC 1 cut(s) 465
MalI GATC 4 cut(s) 110, 516, 632, 758
MboI GATC 4 cut(s) 108, 514, 630, 756
MboII GAAGA 2 cut(s) 397, 442
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 58
MluCI AATT 3 cut(s) 248, 293, 425
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 1 cut(s) 194
MseI TTAA 2 cut(s) 195, 674
MspI CCGG 2 cut(s) 22, 316
MspR9I CCNGG 3 cut(s) 22, 63, 350
MvaI CCWGG 2 cut(s) 63, 350
MwoI GCNNNNNNNGC 4 cut(s) 388, 539, 601, 739
NciI CCSGG 1 cut(s) 22
NdeI CATATG 1 cut(s) 289
NdeII GATC 4 cut(s) 108, 514, 630, 756
NlaIII CATG 5 cut(s) 100, 245, 338, 599, 611
NlaIV GGNNCC 2 cut(s) 55, 516
NmuCI GTSAC 1 cut(s) 465
NspI RCATGY 2 cut(s) 338, 599
PaeI GCATGC 1 cut(s) 599
PfeI GAWTC 2 cut(s) 253, 547
PfoI TCCNGGA 1 cut(s) 20
PkrI GCNGC 2 cut(s) 126, 587
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PshAI GACNNNNGTC 1 cut(s) 711
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 61, 348
PspGI CCWGG 2 cut(s) 61, 348
PspN4I GGNNCC 2 cut(s) 55, 516
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 2 cut(s) 155, 395
RsaNI GTAC 2 cut(s) 154, 394
SaqAI TTAA 2 cut(s) 195, 674
SatI GCNGC 2 cut(s) 125, 586
Sau3AI GATC 4 cut(s) 108, 514, 630, 756
Sau96I GGNCC 1 cut(s) 65
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 3 cut(s) 22, 63, 350
SduI GDGCHC 1 cut(s) 58
SfaNI GCATC 1 cut(s) 520
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
SphI GCATGC 1 cut(s) 599
Sse9I AATT 3 cut(s) 248, 293, 425
SsiI CCGC 2 cut(s) 298, 691
SspMI CTAG 2 cut(s) 141, 345
StyD4I CCNGG 3 cut(s) 20, 61, 348
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 2 cut(s) 459, 500
TaqI TCGA 2 cut(s) 41, 111
TasI AATT 3 cut(s) 248, 293, 425
TatI WGTACW 2 cut(s) 153, 393
TfiI GAWTC 2 cut(s) 253, 547
Tru1I TTAA 2 cut(s) 195, 674
Tru9I TTAA 2 cut(s) 195, 674
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 2 cut(s) 124, 585
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 5 cut(s) 61, 78, 306, 389, 591
XceI RCATGY 2 cut(s) 338, 599
XspI CTAG 2 cut(s) 141, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.